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No Paragraph Breaks On The HomePage Only
When I write a new post I just use the WYSIWYG editor so that paragraphs can be separated automatically.
In my most recent blog, I write the post and the paragraphs are there in the preview and in teh single post page, but the homepage doesn't have any breaks between paragraphs.
To make it even more strange, when you "View Source" on teh fomepage, the and the are there. I'm not sure why that is.
I wonder if anyone could tell me where I could go to troubleshoot this. Any suggestions? Thanks.
(PS, the site is http://www.freemacphoto.com )
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Cruz, Manning right back at it for Giants
Credit: The Associated Press
PITTSBURGH — So much for Victor Cruz's holdout harming his chemistry with Eli Manning.
The New York Giants' wide receiver — who missed organized team activities and minicamp while trying to hammer out a new deal — still has some of the fleetest feet in the NFL. Some of the fanciest too.
|The article you requested is no longer available on BostonHerald.com.

subset: commoncrawl


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Miu Miu
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Szohr was joined by <i></i>Kaylee DeFer, although we never actually saw them in each other's orbit.
We'd been a little worried about former Fashion Week mainstay Jessica Szohr, whose ubiquity took a hit once her character was written out of Gossip Girl. But when she arrived at Chris Benz's presentation Monday afternoon, Szohr looked none the worse for the decreased spotlight—in fact, not only was she well turned-out in casual pants, leopard shoes, and a blazer with leather lapels, but her hair was better than we've seen it in ages. From this, we extrapolate that, all along, it was Vanessa From Brooklyn (to quote those awkward SoBe radio spots) who was to blame for those ratty extensions, and perhaps Szohr secretly is relieved her alter-ego fled to Spain so that she no longer has to wear three tons of rope attached to her head.
Szohr was joined at the show by Gossip Girl's Kaylee DeFer, although we never actually saw them in each other's orbit, so we have no idea if it was awkward or not—in fact, for all we know, they hugged like old school chums and traded stories about Blake Lively's hair. DeFer, in fact, has a red dye job now, and we overheard her telling a girl who we think was an event staffer that she'd be attending the party later. "I hope you have drinks," she added, in a sympathetic way that suggested she thinks all PR and organizational types at Fashion Week desperately need to kick back with a vat of gin and a bendy straw. She's probably right.
Our favorite sighting, as ever, was Johnny Weir—a beacon of kooky color in a plaid jacket and red trousers with a thick stripe down the outside that reminded us of the pants the royal guardsmen wear in England while they're standing outside Windsor Castle trying not to smile when tourists goad them. He also had on a floral shirt and green shoes, the latter of which was an improvisation. "Chris picked it all out. I just went to the studio and grabbed everything except for the shoes—I can't walk in the heels," Weir said. What? Scandale! "No, don't worry, I can totally walk in heels. I just can't walk in Chris's heels. They're crazy high," he explained. With his new husband nearby, Weir beamingly discussed his recent marriage and upcoming plans for a celebratory big-bash wedding. "We're gonna blow it out, Johnny Weir-style. I don't do anything half," he said. "It's going to be next fall or winter. I'm thinking a possibly skating-related celebration." Oh, please, put the bridesmaids on ice. Wait, hang on: "They'll be maids of honor. I don't need bridesmaids, I need the 'of honor.'" Fair enough—then we'll hope for Bridesmaids on ice instead. (For more Fug Girls, go here).
Why Everyone Loves the Alpha Girl
Will We Abandon Women's Rights in the Name of Progressive Politics?
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When Women Pursue Sex, Even Men Don't Get It
Steve Bannon Unironically Referred to Megyn Kelly As 'The Devil'
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A Funny Thing Happens When Women Begin to Outearn Their Husbands
Girlboss Will Make You Nostalgic, But Maybe Not in a Good Way
Serena Williams and Alexis Ohanian Are Taking a Well-Deserved Vacation
Here's Chelsea Clinton Responding to an Awkward Joke About Her Mom
To be fair, it was a 13 foot-long dress.You and Rihanna Will Both Want to Invest in Dior's New Bag
It's got something for everyone.Polo Shirts Have Turned Their Back on Ryan Lochte
Along with his other major sponsors.Ryan Lochte Will No Longer Be Paid to Wear Tiny Bathing Suits
Speedo remains committed to transparency.Laura Brown Is the New Editor-in-Chief of InStyle
After 11 years at Harper's Bazaar.Tyra Banks Is Going to Teach a Class on Smizing at Stanford
"If I see somebody not paying attention, I'm gonna call on them."This Floating Pier Is the Most Zen Installation Ever
Walking on water in Italy.Nation Is Appalled by Matt Lauer's Nude Ankles During Ryan Lochte Interview
What's the opposite of "Jeah"?8 People at the Life of Pablo Pop-up Explain Why Kanye West Is a God
"I mean, Kanye West is just Kanye West. There's not more or less you can say about Kanye West. He's just Mr. West!"A T-shirt Is Enough
Simplicity, versatility, and cool. What more could you want?
She took a perfect pencil dive off a 30-foot yacht.American Apparel Is Being Sued by Former Workers
As the company considers putting itself up for sale.A Gendered History of the Tailored Suit
From Marlon Brando to Coco Chanel.How Zendaya Developed Such Great Style at the Young Age of 19
The star's best looks from Disney to now.Proof That If You're Chic Enough, a Little Federal Investigation Doesn't Matter
Is this the best they could do?5,300-Year-Old Mummified Iceman Probably Would've Been a Street-Style Star
He had several different looks and was "pretty picky."J.Crew Has Identified 226 Shades of Pink
Even more than there are shades of gray.Gigi and Bella Hadid Merch Is Now Somehow a Thing That Is Happening
Today in Hadidiana.Gird Your Loins for the Return of Yeezy to New York Fashion Week
The season approaches.This Indie Brand Had a Great Response to Ivanka Trump
When she bought one of their cuffs, they donated the proceeds to the Clinton campaign.


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A Garuda Indonesia 737 landed safely in Pontianak, Indonesia, after its pilot collapsed and died during the flight. The co-pilot took over and returned the plane, which was on its way to Jakarta, back to Pontianak without incident. Well, without further incident, as the pilot dropping dead was an incident.
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Ellumiglow Partners With Lumilor To Distribute EL Paint Across North America
Friday, April 21, 2017 10:47:58 AM America/Los_Angeles
Ellumiglow is excited to announce a new partnership between Lumilor to distribute EL Paint products across the United States and North America. With a specialized mixture that allows you to light up just about any...Read More
Top LED Strip Lights of 2017
Wednesday, November 30, 2016 9:34:19 AM America/Los_Angeles
Our sales staff has been asked a countless number of times, how do you make a perfectly diffused LED Light that is slim, flexible, and a neon replacement. Our solution? The 24V Wavelux LED Diffused Strip Light.Read More
VynEL™ Now Available For Purchase
Wednesday, November 9, 2016 11:16:01 AM America/Los_Angeles
The worldwide release of the much anticipated integrateable light is here. VynEL™ is now available for purchase! Check back to our education center over the next few weeksRead More
Introducing: VynEL - The Most Versatile Light Ever!
Tuesday, July 26, 2016 4:50:41 PM America/Los_Angeles
Coming soon to Ellumiglow, the future of wearable lighting. We are proud to welcome VynEL, a versatile lighting product made specifically for wearables in mind. Flexible, crushable, durable, oh, and machine washable!Read More
Unlocking The Full Potential Of Laser Wire™
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This Week In Lighting - Episode 1
Sunday, December 13, 2015 9:40:23 AM America/Los_Angeles
This Week In Lighting, Justin shares some of the newest lighting technologies in the accent world. White Laser Wire, which is a thin, optical glass that lights up wonderfully is the first thin lighted wire...Read More
White Laser Wire - The Moment Many Have Waited For
Wednesday, September 16, 2015 4:32:20 PM America/Los_Angeles
Long has the world of lighted wires longed for a true white color, and we've finally made it! Brilliant in all its glory, the new WhiteRead More
Introducing Laser Wire
Tuesday, June 23, 2015 4:25:21 PM America/Los_Angeles
We are happy to introduce the worldwide release of our new product, Laser Wire. It's a revolutionary new product that is soon to replace many EL Wire applications as we know it.Read More
Vivid Illuminates Sydney
Wednesday, June 10, 2015 11:11:40 AM America/Los_Angeles
The World's Largest Lighting Festival, Vivid Sydney just finished with an amazing show of light. The 7th annual festival spanned across 10km over the city of Sydney, Australia.Read More
Inflatable Domes Make Superior Pop-up Advertising
Wednesday, May 27, 2015 11:07:08 AM America/Los_Angeles
Inflatable structures are easy to set up, weather proof and mobile, coming in dynamic, eye-catching designs and colors...Read More


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StarCraft II: Heart of the Swarm will introduce a few new units to game's tried and tested roster. Normally you'd have to wait for the actual game/expansion for this, but why wait when there are mods?
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text: What is the meaning of a final class and a final method? PHP 0 avneesh 303 Rep. January 31, 2017 What is the meaning of a final class and a final method? Vidhi answered January 31, 2017 1 answer ActiveVotedNewestOldest 0 Vidhi 321 Rep. January 31, 2017 'final' is introduced in PHP5. Final class means that this class cannot be extended and a final method cannot be overrided. Vidhi answered January 31, 2017 Please Login or Sign up. × Login Username or Email Password Remember Me Follow 1 Question stats ActiveJanuary 31, 2017Views198 timesAnswers1 answerFollowers1 follower Share this post: on Twitter on Facebook on Google+


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You state that claret is "tough when young" - yet most of the wines rejected in the Which? test are less than four years old. Of course they will not seem attractive when compared with early maturing styles from the New World.
Similarly, Anthony Rose is wrong to assert that the "New World beats Bordeaux at its own game". It is playing a very different game indeed. No claret drinker would regard "intense fruit flavours" as a priority; rather, one looks for the subtlety and complexity that the climate and terroir of Bordeaux often provide.
There is plenty of good value claret available from competent petits chateaux. At the same time, there is plenty of poor, additive-rich wine emerging from Australia.
Enfield, MiddlesexReuse content


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These silky velvet lounge shorts are opulent and romantic, and featuring a stretchy smocked waistband, silk drawstring ribbons, scalloped lace trim, and side overlay for a subtle peek-a-boo effect.
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Sound Living of the greater Chicago area is a true example of a premier home automation and home audio integrator. New to MSE Audio last year, Sound Living has made quite an impression on our sales team, making a number of high dollar sales from the Phase Technology line, including some Atmos dARTS systems – very complicated, truly customizable sound systems. We have been impressed by Sound Living's can-do attitude when it comes to these high-end and rather complicated installations. If you live near Chicago, stop by the Sound Living studio in Highland Park, IL., where several Phase Technology speakers are on display and can be demoed.
1550 Berkeley Road
Highland Park, IL 60035


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Reviewed By Chase B (Bentonville, AR) Reviewed for a 2016 GMC Canyon —4/13/2017 9:05 PM
Took me about an hour to put on while talking to a neighbor. Most difficult part of assembly is how to install the saddlebag, as model-specific instructions were missing in mine. That being said, after a quick adjustment and 2 weeks of constant rain I have had no moisture to mention in the bed. Very pleased with it overall.


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Walz Scale provides a large choice of industrial bench scales suitable for your particular needs and environment. With available capacities from 1lb up to 600lbs and our bench scales' rugged construction, Walz Scale can supply you with a bench scale system that meets your application. All Walz Scale bench scale products offer maximum operator convenience with outstanding displays and innovative weighing functions for high efficiency in daily work as well as ingress protection up to IP69k for heavy wash down applications.
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In addition offering one widest selections of truck scales for sale, we have one of the largest global service and support organizations for on-board scales, portable truck scales and mining scale applications. Whether you need truck scales for rent or are looking to invest in a fixed weighbridge truck scale for long-term use, we provide individually-tailored solutions based on your unique needs.
We focus on the development of new advanced weighing technologies that provide exceptional value to our customers. Our on-board truck scales are amongst the broadest in the world, with thousands of systems supplied globally. From wheel loader scales to portable mining scales and rail scales, Walz Scale is a trusted supplier of a number of industrial weighing solutions.
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Whether for agricultural and farming to resource aggregation and waste management, our load (or harvest) management solutions meet the needs of many industry leaders. Our load management technology and software platforms are often integrated with various wheel weighers and truck scales to offer a highly-optimized solution for maximum throughput.


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Mr. Peabody & Sherman Junior Novelization (Mr. Peabody & Sherman)
Erica David & Random House
This book is available for download with iBooks on your Mac or iOS device, and with iTunes on your computer. Books can be read with iBooks on your Mac or iOS device.
DreamWorks Animation brings Jay Ward's classic cartoon Mr. Peabody & Sherman to the big screen in an all-new comedy adventure for the whole family. Mr. Peabody is the world's smartest person who happens to be a dog. When his "pet" boy, Sherman, uses their time-traveling WABAC machine without permission, the events in history spiral out of control to disastrous and comical results! It's up to this most unexpected of father-son teams to put things back on track. Mr. Peabody & Sherman: The Junior Novelization retells the whole exciting story for boys and girls 7–10 and features eight pages of full-color images from the movie!
- $5.99
- Category: Fiction
- Published: Jan 21, 2014
- Publisher: Random House Children's Books
- Seller: Penguin Random House LLC
- Print Length: 128 Pages
- Language: English


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31310 Alvarado Niles Rd, Union City, CA 94587
(510) 489-4359
31310 Alvarado Niles Rd, Union City, CA 94587
(510) 489-4359
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08/05/2009Provided by Citysearch -
iFly is an amazing experince! You must try it!
I was so excited to try iFly since it opened a while back, and finally got the chance. I had read reviews about it before and wasn't sure what to expect.
The staff was super friendly and made us feel really comfortable with flying. The 1 minutes flys are exhilarating to say the least, lol! I was able to fly without the instructor holding onto me on my first try!
The prices are fair and the photos afterward are hilarious lol. I will be doing this again.
07/08/2009Provided by Citysearch -
I am hooked. This is really a blast.
My whole family went for the first time 2 weeks ago (Mom, Dad, and 7 yr. old son). We've been back three times since and will return at least four more times over the next month or so.
The staff are very helpful and really work hard. Without exception, they really try to please. The check in process is very smooth and their explanation of what to expect is very good.
On my second visit I was so proud to have improved to the point that I could fly where ever I wanted and even flew to the exit under my own power. All due to the great instructors.
In my view, the only downside is that their packages are pretty inflexible. I would love to go a lot more often and am willing to shell out a bunch of money up front to buy hours in advance... but they lack any practical plan that meets that need, Bummer, because if they had such a plan, I'd bring 6-8 new friends in every week.
That said, the amount of freefall time you get for you $ is a lot more compared to the amount you get on a skydive.
It is a great way to increase your freefall skills in a safer environment than in the sky, which is a great confidence builder.
I went to i FLY SF Bay after my second day off AFF. I got really unstable and lost both of my instructors. My instability caused severe line twist in my parachute. I had to cut away my main and deploy my reserve.
After that, I went with one of my instructors to i FLY. The staff there worked with me to teach me how to remain stable on my belly, even while pretending to check my altitude and deploying my parachute. I gained enough confidence to return to the sky the next day, and have a great skydive!
I went back again within the month to practice turning, so that I'd feel even more confident with my abilities. I finished AFF within a few days from that. Now, I have my A Lic! Now that I'm flying well on my belly, I would love to return to i FLY to learn how to back fly, sit fly, and fly head down!
Thanks to the staff for all your help!
I'm an outdoor skydiver with 18 years and 3,500 jumps under my belt. While there is no experience quite like jumping out a plane, iFly SF Bay provides a totally authentic re-creation of the freefall experience. So authentic in fact that outdoor skydivers use facilities like this to train their flying skills for competition. You aren't competitive anymore unless you cross train indoors, it's that good. Any of the flying acrobatics you've seen can be safely learned and practiced indoors in a totally controlled environment. It's incredibly fun, totally spectator friendly, and accessible by people of all ages from three and up!
iFly is a great way to decide wether or not you would want to do real skydiving, because it shows you just what it's like! The employees are totally helpful and show you all the tricks and it's so much fun!
I have over 1300 skydives and I truly love iFlySFBay. I just can't get enough. I've taken many non-skydivers and they to have loved it -- from 3 yrs old to 60+, they all had a great time.
Just book in advance because the weekends are busy. And my recommendation for the first flight is to book for 4 mins of flying. 4 mins of tunnel time is about the same as 6 student skydives of airtime.
Hope to see you there.
My daughter and I went for the first time yesterday. It was a BLAST! We will go back and take a few more family members with us real soon!!! Thanks to our instructor Tom is was freakin AWSOM!!!!
03/06/2009Provided by Citysearch -
I have been skydiving a few times before and loved it, when I heard about this indoor skydiving I had to try it! It is so much fun, totally similar to the real thing. I was able to improve my flying, with the help of one of the instructors. I can't wait to go back


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Posted 6/9/16 at 3:33 PM | Mark Ellis
By Mark Ellis
When the wind and the waves threatened to swamp a boat filled with refugees fleeing the Middle East, Jesus made a dramatic appearance to them and calmed the waters, saving their lives.
The truly amazing account is from Erick Schenkel, executive director of the Jesus Film Project.
"A group of refugees fleeing the fighting in the Middle East were jammed into several pontoon boats. They were trying to make it across the Aegean Sea to Greece," Schenkel recounts.
The seas were extremely rough and dangerous and some of the boats in this small armada capsized.
The report is reminiscent of a similar storm on the Sea of Galilee described in the Gospel accounts, when "A furious squall came up, and the waves broke over the boat, so that it was nearly swamped." (Matthew 4:37)
The refugees were afraid, just as Jesus' fellow travelers were frightened. FULL POST
Posted 6/8/16 at 12:24 PM | Karen Woodall
This week's news has been dotted with stories covering the life and career of former Heavy Weight Boxing Champion Muhammad Ali who passed away last Friday from protracted health issues. Arguably the first of a vanguard of flamboyant 'trash talking' athletes, Ali was known for taunting his opponents openly both inside and outside the ring, boldly declaring himself to be "the Greatest of All Time." And, you know, he was right… for a while.
But that was a long time ago, and as he learned, (and the rest of us will learn if we live long enough,) time has a way of stealing away things in which we once took great pride and satisfaction. Oh, these things don't have to be limited to athletics. They can be beauty, intellect, dexterity, imagination, creativity, or a host of other things. Even for those who are skilled and fortunate enough to somehow against all odds reach the pinnacle of their career, and to ride the fading wave of fame and fortune while garnering the admiration of millions, they all soon find out that being "the greatest" just isn't that great anymore. FULL POST
Posted 6/5/16 at 8:04 PM | Brittney Moses
I'd like to think I have a pretty wholesome attitude and state of mind in general. However, I am in no way immune to breaking down.
I'm not perfect (surprise).
I get stuck in my fears and my doubts.
I've felt hopeless.
I've hit my ultimate low on many days.
I've been trapped by the expectation to set an example.
I've depended far too much on myself to get this thing right.
I have handled my flaws legalistically rather than with grace.
(And trust me, my flaws are many.)
I learned to be resilient and strong in the Lord, but I had lost the beauty of being broken.
We tell people to come to Christ as they are but the image we present doesn't match our words. In perfectly groomed clothes, photo shopped images and the lack of sharing our present day struggles, we subconsciously make ourselves the standard. We praise the good but when the bad and the ugly actually come out we're quick to shame and judge another's walk with Christ. Since when did imperfection and human flaws become such a shocker among the body? Everyone is going through something behind closed doors, including those we admire the most. FULL POST
Posted 6/2/16 at 7:42 PM | Mark Ellis
By Mark Ellis
John Robertson McQuilkin, the college president who stepped down to care for his wife stricken with Alzheimer's, passed into the arms of Jesus on June 2nd. He was 88.
McQuilkin had served as president of Columbia Bible College and Seminary for 22 years (now Columbia International University) when he resigned in 1990 to care fulltime for his wife, Muriel, who suffered from Alzheimer's Disease.
He met Muriel at the school when they were students. When they first met, he discovered she was "delightful, smart, and gifted, and just a great lover of people and more fun than you can imagine."
He proposed on Valentine's Day in 1948 and over the next 30 years, they raised six children and served God in many ways, including 12 years as missionaries in Japan.
In 1968 they returned to the U.S. and Robertson became president of the college. Muriel also taught there, spoke at women's conferences, and on TV and radio programs. FULL POST
Posted 6/2/16 at 8:18 AM | Chris Fedorcek
Life has a funny way of throwing things at us when we least expect it. Maybe it's a blown engine, the loss of a job, a death of someone close to us or a diagnosis that wasn't expected. For many, especially men, our first response is, "What do I need to do to fix it?" There's an obvious problem so therefore there must be a solution, something that I can do, to overcome whatever has been put in front of our path.
Too often we try to lean on our own strength or head knowledge to overcome something or provide a need that only a loving Father in heaven can provide. I love 2 Chronicles 20:15 when the Lord tells Jehoshaphat, "Do not be afraid or discouraged because of this vast multitude, for the battle is not yours, but God's." He continues in verse 17 to say, "You do not have to fight this battle. Position yourselves, stand still, and see the salvation of the Lord. He is with you, Judah and Jerusalem. Do not be afraid or discouraged. Tomorrow, go out and face them, for the Lord is with you."
You see, regardless of the fight that is ahead of you, the posture of our heart must remain focused on and confident in the Lord that his provision will come through and that it will sustain us. One of the struggle points to remember is that His answer, His provision, may not always look like what we imagine in our mind, but His response is the perfect response based upon His will, not ours. FULL POST
Posted 5/19/16 at 3:49 PM | Karen Kramer
I love beach walks; the salt air clears my mind. It's also my go-to place when I'm thinking about those who are struggling. I have a friend whose journey is filled with anguish. Her future could get better, but she can't seem to move forward. I can relate.
Some people get frozen in bitterness, others are weighed down with regrets. For me it was self-loathing. For her, it's debilitating sadness.
For some, moving forward into the unknown seems worse than staying someplace unhealthy. But for things to get better, change has to happen, even when it's hard and a bit scary.
They say that the things that break us can make us better people. But who wants to be broken?
Well, at the beach we can take a lesson from the crab.
As a crab grows it must periodically shed its hard shell. Keeping the old one isn't an option. Shed it or die. FULL POST
Posted 5/16/16 at 9:59 PM | Chris Fedorcek
"Therefore, submit to God. But resist the Devil, and he will flee from you." (James 4:7 HCSB)
Let's be honest and take down the facade for just a few moments today...some days are hard! There are days that I don't feel like putting my best foot forward and days I wonder why God called me in to ministry. There are days I sincerely question if I am accurately reflecting the peace, love, grace and joy of Christ within me. I often feel as though for many followers of Christ we tend to run our "me and Jesus" relationship at about a 60/40 ratio, with the majority being a self-centered, convenient form of what we justify as the Christian life.I would say, for many, that on a good day we run about 90/10, which in theory sounds good right? I mean, that 90%, that's passing...that's an A!
Here's the thing, I don't recall reading anywhere in scripture that we are called to submit part of our life to Christ. Christ didn't give bits and pieces of Himself for us when it was convenient for Him. Christ fully submitted all of Himself to the Father so that we may walk in freedom and be covered by His grace. Just as Christ was called to be all in, we are called to be all in, one-hundred percent, one-hundred percent of the time. Living a life for Christ isn't a 50/50 relationship that involves mutual submission. Living for Christ is submitting all of you for all of Him. It's giving up complete control; our thoughts, our actions and our words and allowing the Spirit of God to reflect out of our hearts to the atmosphere and people around us. FULL POST
Posted 5/3/16 at 8:17 PM | Greg Holt
Lord Lead Us
Where does your heart live – do you live for now, or the future? Or are you caught up in yesterday? Where we live is not so important as how we live – where our hearts reside.
It is so easy in this fast paced world to get caught up the rush of life losing what the reality of our lives should be.
Lord lead us back to You.
The day starts with good intentions and becomes over-whelmed with busy. Work intrudes its ugly head with that 'gotta get it done' problem. The car broke down – our child is in trouble – the furnace just died.
Lord lead us back to You.
We mean to read the Bible but just don't get to it. We mean to pray – pray for that one person that really needs it – for our country, but prayer gets forgotten in the 'to do list'.
Lord lead us back to You.
Let us resolve to make today different – today let us take that time – time to read the Word of God. Let us take the time to pray, not only for others, as we should – but to thank the Lord for His goodness towards us. FULL POST
Posted 4/29/16 at 4:11 PM | Mark Ellis
By Mark Ellis
A four-year-old Golden Labrador Retriever that helped save earthquake victims trapped under rubble died from heatstroke while performing his duties.
Dayko, a valuable member of the fire department's K9 unit, was part of a search-and-rescue team searching for survivors following the 7.8-magnitude earthquake that hit coastal Ecuador, about 100 miles from Quito, according to the Washington Post.
Dayko was invaluable crawling through the debris and rubble, sniffing out survivors. Amazingly, he was able to save seven people before his own death.
The Ibarra fire department said Friday that Dayko died from heatstroke after local veterinarians desperately tried to save the animal, according to the Washington Post.
The department said an autopsy showed he died after suffering a massive heart attack and acute respiratory failure. FULL POST
Posted 4/28/16 at 4:06 PM | Karen Kramer
Mount Storm King in the Olympics is a quick but intense climb since it's 1200 feet of elevation per mile. But the last 500 feet truly test your limits.
It was Christmas Day, and as a gift to myself, I wanted to hike to the top.
The Very Top.
Beyond the end-of-trail warning sign, ropes are securely (?) tied to outcroppings enabling the fearless to climb to the summit.
From there one can see the entire glacier-fed Lake Crescent and beyond to the Strait of Juan de Fuca, the watery dividing line between the United States and Canada.
A bonus on this Christmas Day climb, was fresh snow on the rugged summit. So, Tommy, my son and official Storm King guide, and I laid fresh boot tracks through a couple feet of snow. FULL POST


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Hello All!
Here is a beautiful couple name Kim and Steve! They got married at the Madison in Covington and had just the greatest wedding. The groom was a hoot, including his groomsmen,( HI SCOOTER!), and we couldnt have have a more beautiful and photogenic bride! Congrats to the two of you and wish you many happy years and a wonderful life together!
Sherri and Christin


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The timing chain slipped on my 98 cavalier. Can I replace it, and if so, how much will it cost me?
My dad is saying that this is the end of the car, and I'm just going to have to junk this
one and get a new one. Is he right or can I fix my car?
2 Answers
It depends on how much damage was done to the valvetrain. If the timing is just off with no collisions between valves and pistons then simply replacing the timing belt and gears and re-aligning the timing marks will solve your problem. If the valves collided with the pistons there could be varying degrees of damage, from bent valve stems and/or pushrods to holes in pistons, smashed valves, and/or broken camshaft(s) or more. I would have the engine taken apart and inspected before deciding whether or not to junk the car.
depends on the motor in the cavalier if u have the 2.2 then the motor should b fine and replacing the timing chain is an excellent idea but if u have the 2.4 quad 4 motor then u more than likely sent the valves thru the pistons b/c it is an intrusive motor
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SilkScape Print Media Suitable for Latex Printing
Tuesday, July 23, 2013
Press release from the issuing company
Richmond, VA - Drytac® has confirmed that SilkScapeTM, the latest product available in its digital print media line, is compatible with latex printers. A profile for the HP Designjet L26500 with Caldera rip software is available upon request. The banner material can also be used with solvent, eco-solvent and UV printers, making SilkScapeTM an excellent low cost solution for print providers.
SilkScapeTM is an 8 mil, 99% opaque white polypropylene film with a satin matte finish and single-sided print receptive coating. It provides outstanding image quality no matter which print technology is used. In addition, the material's edges are curl-resistant and do not fray, making it an ideal choice for use with the Drytac® line of retractable banner stands.
"SilkScapeTM has fantastic print quality and hangs nicely in the stand. It is a great option for customers who are looking for a cost-effective solution that acts like a higher-end product," said Nate Goodman, Drytac® Product Manager.
Available in 36-inch wide rolls, SilkScapeTM is in stock and ready for immediate shipment. To learn more, visit http://www.drytac.com/silkscapetm.html.
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$30 pickup at REIShop Now
Hydro Flask 64oz Wide-Mouth Insulated Bottle
Last verified
REI Garage offers the Hydro Flask 64-oz. Wide-Mouth Insulated Stainless Steel Bottle in Stainless or Black for $29.73. Opt for in-store pickup to dodge the $5.99 shipping charge. That's tied with last week's mention, the lowest price we could find today by $35, and a great deal for an insulated stainless steel bottle of this size. It features double-wall vacuum insulation that keeps liquids cold for up to 24 hours and hot for up to 12 hours.


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Need a book? Engineering books recommendations...
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RE: Wood Beam Torsion[Subject Prev][Subject Next][Thread Prev][Thread Next]
- To: <seaint(--nospam--at)seaint.org>
- Subject: RE: Wood Beam Torsion
- From: "Dennis Wish" <dennis.wish(--nospam--at)verizon.net>
- Date: Wed, 8 Oct 2003 11:26:17 -0700
I actually resolved this. The model code used required only a calculation for a 200 pound lateral load applied at the top of the railing 42 inches above the deck. In this case it resolved into a reaction in tension of close to 1100 pounds depending on the applied direction of the 200 pounds (in or outward). The torsion issue is small as the aluminum track for the glass rail is bolted through the rim beam at 12-inches on center and the code also says to check for shear based on the application of 50-plf laterally at the top rail (whichever is worse). The assumption is that the glass comes in 48" sections and this is the worst case load when considering the bending arm. The shear is low – 50 pounds between the wood beam and bolted connection.
In short – the beam has a tendency to pull away from the face of the TJI joists when a lateral load is applied at the top of the rail. Therefore, I used a Simpson LTT19 at the top and bottom and spaced it 48 inches on center to resist the tensile reaction. The strap is bolted through the 4x beam and the washer and nut are recessed so that lath and stucco can be used to conceal the connections.
Thanks again for your help – I think I have this one back on track (pun intended)
-----Original Message-----
I would like to help but I'm not sure I understand how the glass rail is supported laterally and attached to the wood rim beam. Is the 600# load the result of the 30' rail span x 20# / ft.? Is the 24# / sq. ft. a wind or seismic load? Normally the top rail spans to line posts at 6' +/- that cantilever off from a rim joist or beam. Is the aluminum track a structural member? Do we finally have structural glass? :>}
Thanks for your help with my wood beam repair.
Ray Shreenan
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Oktoberfest draws thousands of visitors to Munich every year at the end of September for the world's biggest folk festival. And these days, women are expected to 'put on their finest!' But it takes more than just a fetching dirndl – you definitely need the right hairstyle to make the best impression at Oktoberfest.
Hair artist Tom Kroboth reveals how to transform the dirndl and hairstyle into a complete work of art with a hair look for the upcoming Oktoberfest season that is both glamorous and fun. He gives us a completely new and modern interpretation of the traditional, classic Oktoberfest style, the side-parted, plaited ponytail:
"This year, Oktoberfest will be colourful, very feminine yet unconventional. Soft pastel tones and expressive neon colours define the look. This has also inspired me to enhance our plait with gentle shades of peach and rosé, which liven up the golden blonde a lot while giving it a sophisticated, modern look. The hair has a touch of the 90s and the more courageous women will dare to wear it with a white-blonde undercut and a pinned-on plait. The layered haircut is perfect for many styling variations and is easy to blow dry yourself.
To give hair some hold while blow drying, I worked in some c:ehko styling mousse at the roots, since it will need stamina and endurance at Oktoberfest.
A medium round brush is used to give hair bounce and volume. The hair at the top of the head is lightly teased with a large brush and styled with the fingers. My tip for finishing is to set with hairspray and put a bit of c:ehko Gel Wax on your fingertips beforehand – this makes styling easier and gives an attractive shine. Then tie the hair first on the top of your head and then at the neck with a hair elastic into a side ponytail and divide into two. Now take a thin strand of hair from underneath and place it over the other side, alternating from one side to the other. This is how you make a fishtail plait, which you then fasten with a clear hair elastic. Loosen the fishtail plait a little with your fingers to make it look natural. Then loosely entwine the end of the upper ponytail with the lower one and fasten with hairpins. Finally, you can put the lower part of the fishtail plait up or tuck it in and secure with hairpins.
My secret tip for those who don't want to colour their hair for Oktoberfest: attach two extensions in different yet harmonising shades to the lower ponytail – this gives you nearly the same result!"
© Photo Credit: PR Sugar GmbH


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Skin care used to be just a matter of vanity. But it can also be a matter of health. Your skin can reflect your health. For example, if you don't take care to protect your skin from the sun, you can be facing skin damage at the least and skin cancer at the worst. This article can help you to know how to care for your skin.
Don't be afraid to shop around for the right skin care products. All skin is different, and there are hundreds of products out there to choose from. There is no need to immediately shop for the pricier products. Start with something cheap from your local drug store. If it works, great! If not, keep experimenting.
To achieve a beautiful face, you should exfoliate a minimum of three times each week. Try a scrub that is made especially for the face. If you have sensitive skin, try to find moisturizing exfoliants. Among the many benefits of exfoliation are clear pores and sloughing of dead skin. By exfoliating regularly, your skin will glow.
Age spots are a form of sun damage that can become more visible as we age. To avoid getting these freckle-like spots, wear a good sunscreen every time you go outdoors. Once you have them, some people get good results with a skin-lightening cream, but you must be consistent with its use, and be patient, as results may take several weeks.
To protect yourself from the sun more efficiently, get some pomegranate pills at any health store. Pomegranate pills make the skin more resilient to the sun. They're natural and completely safe. They will make your skin healthy and looking great.
As the above article warned, skin care can be a matter of beauty and a matter of health. Taking care of your skin promotes beauty and even good health. By heeding the advice in the article and caring for your skin, you will find that your skin will reflect this and you will have beautiful skin for a lifetime.


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Joico Creme Wax Texture & Shine 50ml
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Joico Crème Wax Texture & Shine is a professional styling aid which gives flexible hold to both textured and smooth styles.
Extremely versatile, Crème Wax Texture & Shine combines the hydrating benefits and shine finish of a cream with the fibrous hold of a wax. Ideal for coarse or heavy hair textures, this innovative styling pomade is enriched with Natural Microcrystalline Wax which delivers a moderate, pliable hold whilst Propylene Glycol adds nourishment and shine to the hair.
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Joico Í The art of healthy hair. The mission of award-winning Haircare range Joico has always been to develop innovative and top performing hair products that you'll love. To help you find your perfect Joico hair routine, simply browse the products using the buttons on the left. We love the Joico Body Luxe Volumizing Shampoo for adding volume and the JoiWhip Firm Hold Designing Foam for long-lasting style creation. Joico also offer the famous K-Pak which is intended to strengthen, rebuild and protect over-damaged hair.
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At Globys, we develop a cloud-based B2B billing portal that allows customers to automate their payment workflows while analyzing and optimizing their utilization of a service. Our billing portal is used by many of the world's largest telecom providers to make their billing a valuable element of the customer experience. We are now expanding into other sectors such as public cloud infrastructure, IoT, logistics, and other industries.
We are seeking a Sr. UI/UX Full Stack Engineer to lead the effort of building the interface for our next generation cloud analytic platform.
As Sr. Engineer at Globys, you will be responsible for:
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At Globys, we bring together energetic entrepreneurially-minded individuals to build amazing products that people all over the world use. As a team, we're really excited about what we're accomplishing. Globys is a profitable, globally admired, engineering-oriented company that is thriving in the very hot telecommunications and mobile analytics space. We're giving our customers what they need the most – deep insight into their data and software to better tailor their services to meet their customer needs. And, we think that's really cool! We're passionate about creating an environment where people love what they do and who they are doing it with. A place where:


text:
Over time some alerts (such as low disk) occur multiple times. It would be helpful to see a track record of repeating alerts. If, for example, a continual low disk situation is being fixed on the same computer/devise then having a history of those alerts will help to justify the expense of upgrading or replacing the device. I can't find a log file that records alert history.
1 Reply
· · ·
Aug 28, 2008 at 1:39 UTC
they may remain in log files, but if you clear an alert in SW, it is gone. This is to the best of my knowledge.


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GARY Keedy has revealed he would have played in the C&G Trophy final, despite the arrival of Kartik Murali.
The Lancashire spinner was ruled out the day before the showdown with Sussex at Lord's with a knee injury.
But all the talk among supporters in the week before the game was that the 30-year-old would be overlooked in favour of Indian left-armer Kartik, who arrived in the country just four days before the final.
But 30-year-old Keedy says he was set to play in the final, only for his knee to give way in the final practice session at Lord's.
"There is no doubt that I would have played," said Yorkshire-born Keedy. "It was crystal cut. I know it looks suspect from the outside because the club have brought in a left-arm spinner from India the week before the C&G Trophy final, but I was going to play.
"I set off to bowl in the nets at Lord's the day before the final and my knee almost gave way. I may have been able to get through a 50-over match, but it was too big a risk for me to play. If it had gone in my first over, then we would have been in trouble.
"I haven't played too much one-day cricket in my career, but I made an impression in the group games and if the final had been played at the end of the group stages, there wouldn't have been a problem.
"But anything can happen in two months, and unfortunately I picked up an injury.
"I had an operation on my knee three years ago, and I was getting similar symptoms to then.
"I remember landing heavily on it in the Championship match at Sussex, and I was complaining about it during the one-day game there.
"In the end, I went for an MRI scan which showed I had a bruised bone which was causing my knee to swell up and I was getting muscle discomfort. Although I am getting similar symptoms, it doesn't need surgery.
"I haven't played any cricket this week and it is coming along nicely."
The injury to Keedy's left knee kept him out of last week's Championship match with Warwickshire and will sideline him for tomorrow's Pro40 floodlit clash with leaders Essex at Chelmsford and Saturday's match with Warwickshire.
But Keedy is hoping to be fit for next week's crucial Championship game with Durham at Old Trafford, where he could be lining up alongside Kartik after the Indian was given the green light yesterday to stay until the end of the season as Brad Hodge's replacement.
And although they are both left-arm spinners, Keedy, who has taken over 450 first-class wickets for Lancashire and was named player of the year in 2004, does not feel threatened by Kartik's arrival.
He said: "The club has made the decision to bring in Kartik, but my stats over the last four years for Lancashire speak for themselves."
Keedy believes the C&G Trophy final defeat by Sussex will have no bearing on the title race.
"The final defeat won't make us hungrier because the hunger has been there all year," he said.
Stuart Law has been rested for tomorrow's trip to Chelmsford, with Steven Croft and Simon Marshall included in the 12-man squad.


text:
Need a book? Engineering books recommendations...
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RE: Core Hole Through PT Slab[Subject Prev][Subject Next][Thread Prev][Thread Next]
- To: <seaint(--nospam--at)seaint.org>
- Subject: RE: Core Hole Through PT Slab
- From: "Paul Feather" <PFeather(--nospam--at)se-solutions.net>
- Date: Thu, 27 Sep 2007 16:17:54 -0700
We typically will let them get within an inch on the regular reinforcing, but hold 3 inches clear of the PT just for safety. If you have to relax the three inch you can, but that's the rule we use. Even with x-ray and GPR there is room for error.
Paul Feather PE, SE
FSRahbar(--nospam--at)aol.com [mailto:FSRahbar(--nospam--at)aol.com]
At one of our projects, the contractor needs to core some holes through the structural slab. The slab has both PT cables and regular reinforcing. Cores are from 2" to 4" in diameter and are 16" to 20" apart in both directions. We are not allowing them to cut any of the reinforcing. However, I am trying to give them a guideline as to the distance from the edge of the cores to the edge of the reinforcing. I remember a 1" dimension had been discusses in the past. Is that sufficient? Any thoughts?
Thank you,
See what's new at AOL.com and Make AOL Your Homepage.
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text: News KIYC: Rahway toxic site cleanup company, Soil Safe, investigated You need the latest version of the Adobe Flash Player to view the video related to this article. Download Now. A federal lawsuit filed in South Jersey raises questions about Soil Safe's previous New Jersey remediation efforts. (3/11/14) Like Tweet Share Pin Email 0 Comments Updated March 11, 2014 8:47 PM CARTERET - Does the company tasked with a controversial environmental cleanup along the Rahway River have a toxic track record? A federal lawsuit filed in South Jersey raises questions about Soil Safe's previous New Jersey remediation efforts, a Kane In Your Corner investigation finds, and some environmentalists say it proves the company cannot be trusted with a project as sensitive as the Carteret cleanup. The former American Cyanamid property in Carteret contains 15 acres of cyanide-tainted sludge, making it one of New Jersey's most toxic sites. The New Jersey Department of Environmental Protection recently issued a permit for remediation to begin on the site. The plan calls for Soil Safe to cap the property with tons of petroleum-contaminated soil and cement. The NJDEP took the action despite repeated warnings from some of its own staff, who said the weight of the soil could have disastrous results, pushing cyanide into the river. The new lawsuit, filed against Soil Safe over its handling of a remediation project in Logan Township, raises even more concerns. Records show Soil Safe was approved to build a 5-foot cap on the Logan property, but the Delaware Riverkeeper Network accuses the company of ignoring that limit. "We believe that they are taking in too much material and they are not properly treating the material to safe standards," Riverkeeper Maya van Rossum says. Bob Spiegel, of the Edison Wetlands Association, says the allegations in Logan prove Soil Safe cannot be trusted with the Carteret project, where exceeding the permitted cap size would increase the odds of an environmental disaster. He accuses the company of "leaving behind a toxic track record." Neither Soil Safe nor the NJDEP, which has inspected the Logan site several times a year for the past decade, would tell Kane In Your Corner how high the cap there is. But in its recent reports, the company has taken to referring to a "minimum 5 foot cap," implying it can dump as much tainted soil as it wants to on the property. "That's a violation of their permit and a violation of the law," van Rossum says. The Riverkeeper also blames the NJDEP for failing to take enforcement action against Soil Safe, which she calls "a violation of their obligation to protect the public." Soil Safe did not respond to specific questions from News 12 New Jersey about either the Logan or Carteret projects. Instead, the company issued a general statement saying it obeys the law and has no outstanding violations. Share on Facebook Tweet on Twitter advertisement | advertise on News 12 Trending Video 2:31 1 600-year-old tree to be removed in Bernards 0:26 2 Police seek person who crashed stolen car into Newark church 1:04 3 Morris County officer faces sexual assault charges 0:25 4 Firefighters clear warehouse fire, road closes 2:32 5 Crews begin removal of 600-year-old tree in Bernards advertisement | advertise on News 12


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Why I vote. This is an attempt to achieve the ultimate in i distillation, condensing a 6,000-word speech to sixth-form students at Twickenham's Orleans Park yesterday (pity them) into a 314-word column – part of the election series by Robert Peston's charity Speaker for Schools...
I don't believe in a single party, I don't support a party, I've never been a party member and probably never will be. I'm not totally convinced by any of them. But I don't need to be, to vote. My argument boils down to this: if you don't vote, you don't matter.
There are five reasons I vote.
1. Look at other countries. We can vote for whoever we want in free elections. Many other people on Earth cannot. Take North Korea: a cult of personality makes the people poorer, sicker and more terrified. Or China, where no form of organised disagreement is tolerated, bloggers are jailed, academics and religious leaders harassed, protests banned, the web restricted. Or Turkmenistan, where President Turkmenbashi blocked the internet, renamed April after his mother, banned dogs, lip synching and beards, suggested people chew on bones to strengthen their teeth and told those living in the desert to learn to ice-skate.
2. British history has been written in the blood of those who died for our democratic freedoms. Fighting Nazi-ism... Mill workers protesting at the Peterloo Massacre... The Suffragettes.
3. If I don't vote, I lose the right to complain with integrity.
4. Voting makes me feel good. It's easy. Voting is a positive choice.
5. Voting does carry power. Politicians aren't all as bad as each other – some of them are worse. Many MPs are superb, dedicated to the public they serve. Voting is a way to reward the virtuous and to punish the greedy and stupid. Things never have to stay the way they are. If we give up, change will never happen. [Phew.]Reuse content


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The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: LP CvV SD KFS. Performed the experiments: LP CvV AK NQ VD DH CH KFS. Analyzed the data: LP CvV AK NQ VD DH SD KFS. Contributed reagents/materials/analysis tools: LP CvV AK NQ DH CH KFS. Wrote the paper: LP SD KFS.
The authors have declared that no competing interests exist.
Centromeres are differentiated chromatin domains, present once per chromosome, that direct segregation of the genome in mitosis and meiosis by specifying assembly of the kinetochore. They are distinct genetic loci in that their identity in most organisms is determined not by the DNA sequences they are associated with, but through specific chromatin composition and context. The core nucleosomal protein CENP-A/cenH3 plays a primary role in centromere determination in all species and directs assembly of a large complex of associated proteins in vertebrates. While CENP-A itself is stably transmitted from one generation to the next, the nature of the template for centromere replication and its relationship to kinetochore function are as yet poorly understood. Here, we investigate the assembly and inheritance of a histone fold complex of the centromere, the CENP-T/W complex, which is integrated with centromeric chromatin in association with canonical histone H3 nucleosomes. We have investigated the cell cycle regulation, timing of assembly, generational persistence, and requirement for function of CENPs -T and -W in the cell cycle in human cells. The CENP-T/W complex assembles through a dynamic exchange mechanism in late S-phase and G2, is required for mitosis in each cell cycle and does not persist across cell generations, properties reciprocal to those measured for CENP-A. We propose that the CENP-A and H3-CENP-T/W nucleosome components of the centromere are specialized for centromeric and kinetochore activities, respectively. Segregation of the assembly mechanisms for the two allows the cell to switch between chromatin configurations that reciprocally support the replication of the centromere and its conversion to a mitotic state on postreplicative chromatin.
The centromere is a strange locus that derives its identity from the proteins that shape it rather than the DNA sequences it contains. It also functions in a remarkably singular way, providing a motor and command control center for the chromosome in conjunction with the kinetochore. Key to centromere identity is the chromatin that comprises it, which has a unique nucleosomal "bead on a string" including a special centromeric histone H3, called CENP-A. Found in alternating clusters of nucleosomes with "regular" histone H3, CENP-A is crucial for propagating centromere identity as well as for regulating kinetochore function. In this study, we have analysed the cell cycle dynamics of CENP-T and CENP-W, another two components of the constitutive centromere associated network. We show that, unlike CENP-A, CENP-T/W are not inherited stringently by daughter cells. Instead, these complexes - which are bound to the interstitial "regular" H3 nucleosome domains - assemble after DNA replication and are required for kinetochore formation. Thus, we propose that a stable CENP-A nucleosome population plays a role in centromere locus inheritance to daughter cells, while dynamic CENP-T/W and H3 nucleosomes provide a cycling function that triggers kinetochore assembly as cells enter mitosis in each new cell cycle.
The centromere is the genetic locus present in a single copy on each eukaryotic chromosome that provides the transmission function of the genome across mitotic and meiotic generations
The composition and molecular organization of CENP-A nucleosomes and their mechanistic contribution to centromere determination in several organisms has been a subject of intensive investigation and debate
A large group of additional proteins assemble on centromeric chromatin in pathways that are dependent on CENP-A and feed back to influence its assembly (interphase centromere complex [ICEN]
The CENP-A nucleosome is capable of multigenerational inheritance
The CENP-T/W complex plays a critical role in mitosis
To investigate whether the CENP-T/W complex plays a templating role in propagating centromere identity, we first examined their regulation during the HeLa cell cycle. The relative abundance of CENP-T (NP_079358.3) and -W (NP_001012525.1) transcripts and protein were examined in synchronized populations of HeLa cells (
(A) HeLa cells were fractionated across the cell cycle using a double thymidine protocol. The relative abundance of CENP-T (red) and -W (blue) transcripts were measured by qPCR. CENP-A (dashed grey) is shown as a reference. No significant periodic RNA accumulation was observed. (B) Protein obtained from cell cycle fractions was examined by Western blot for CENP-T and CENP-W relative to tubulin (loading control), cyclin B, and phospho-histone H3 (Ser10). (C) The relative abundance of centromere-associated CENP-W was estimated using a cell line constitutively expressing a CLIP-tagged fusion protein. CENP-W-CLIP was labelled with CLIP-505 at steady state and fluorescence intensity quantified. Cells were counterstained for CENP-A to define centromeres and for PCNA and phospho-histone H3 to resolve the cell cycle stage of individual cells (see
Cells depleted of CENP-A exhibit a distinct phenotypic response, maintaining normal kinetochore function for two to three cell cycles until a critical threshold of about 10% of normal levels are reached and mitosis fails
(A) CENPs -T and -W were depleted by RNAi in HeLa cells, resulting in defects in spindle assembly assayed by immunofluorescence for tubulin (green) and centromeres (red) with DNA stained with DAPI (blue). CENP-W depleted cells exhibit a high frequency of multipolar spindles. Depletion of CENP-T was less severe resulting in characteristic fusiform spindle structures. (B) CENP-W is required for robust mitosis in each cell cycle. Mitotic kinetics were assayed using histone H2B-GFP HeLa cells treated with CENP-W siRNA for 48 (blue circles) or 24 h (red triangles), scoring time between NEB (defined as onset of deformations in nuclear chromatin) and anaphase onset and are plotted by rank order of individual cells. Mean time for scrambled siRNA control (dark X) and untreated cells (light X) is shown as a green line, with +1 standard deviation (SD) marked with a red line. Inset graph reports the percentage of cells with NEB-anaphase times in excess of +1 SD and the average delay in anaphase onset. (C) Experimental schematic describes the pulse chase approach to assay the heritability of CLIP tagged CENPs -T and -W. (D) CENPs -T and -W do not persist at centromeres. CLIP signal intensity coincident with centromeres was quantified at the time of pulse and 24 h later. While CENP-A-SNAP signal was depleted by approximately 50%, as expected, CENP-T-CLIP and CENP-W-CLIP signal was reduced to background levels after 24 h.
A different perspective on the role of the CENP-T/W complex in locus maintenance was gained by examining multigenerational stability. A pulse-chase experiment was performed using cells expressing CLIP-tagged CENPs -T or -W in conjunction with SNAP-tagged CENP-A, synchronizing cells with a double thymidine block and labelling with a fluorescent ligand at the time of release. Cells were harvested at 24-h intervals and the abundance of protein at centromeres was determined by quantitative fluorescence microscopy (
In order to examine the time at which the CENP-T/W complex assembles in the cell cycle, a conditional labelling strategy was employed, similar to that used to pinpoint CENP-A assembly timing in HeLa cells (
(A) Schematic description of the CLIP quench-chase-pulse-chase experiment used to assay the timing of assembly of CLIP tagged CENPs -T and -W at centromeres using synchronized HeLa cells. (B) CLIP-tagged CENPs -T and -W are localized at centromeres prior to the onset of anaphase, indicating that newly synthesized CENP-T and CENP-W assemble at the centromere in the proximal cell cycle. (C) Progressive assembly of pulsed CENP-T and CENP-W in S-phase and G2. Cells were labelled with PCNA and phospho-histone H3 antibodies to document position in the cell cycle. Cells judged to be in earlier stages of S-phase have no detectable CLIP signal at centromeres, while cells later in S-phase and G2 have robust centromere-associated CLIP signal. (D) Centromere-associated CENP-T-CLIP and CENP-W-CLIP fluorescence were quantified relative to progression through the cell cycle, showing an increased signal intensity at centromeres coinciding with progression through S-phase and G2.
To resolve when CENP-T and CENP-W are assembled within the proximal cell cycle, the CLIP-quench-chase-pulse experiment was repeated and the cells were counterstained with centromere (CENP-A) and cell cycle markers (PCNA and phospho-histone H3;
To exclude the possibility that the observed assembly was an artefact of initiating the pulse during S-phase, a comparable pulse-labelling paradigm was employed using asynchronous cells and processed for cell cycle analysis as described above (
(A) Asynchronous HeLa cells expressing CENP-T and CENP-W CLIP were used to assay timing of assembly in an unperturbed cell population. Cells were cell cycle staged by counterstaining with PCNA and phospho-histone H3. (B) Cells were classified on the basis of CLIP signal at centromeres and cell cycle stage. (C) FRAP of GFP derivatives of CENP-T only occurs during late S-phase and G2 indicating loading takes place during this period. (D) Centromere-associated GFP-CENP-T was photobleached and allowed to recover to approximately 40%. Following a second bleach event, recovery continued reaching approximately 40%, indicating an exchange-based dynamic loading process during this time period. (E) Double FRAP of GFP-CENP-W, as for CENP-T. (F) Fluorescence loss after photoactivation. Photoactivatable GFP-CENP-W was activated in G2 cells. Following a chase period of 3 h, the majority of the fluorescent signal had dissociated from the centromere.
An independent method was employed to observe CENP-T and -W assembly dynamics in living cells using fluorescence recovery after photobleaching (FRAP). Centromere labelling with GFP fusions of CENP-T or -W was induced by transient transfection into HeLa and HEp-2 cells and mCherry-PCNA was cotransfected as a marker to allow estimation of the cell cycle stage of individual cells
While the precise mechanism that accommodates replicative dilution of CENP-A is not known, it most likely involves nucleosome assembly. How do CENP-T and -W interact with this assembling compartment? One possibility is that the CENP-T/W complex assembles to a fixed stoichiometry along with new histone H3 nucleosomes. CENPs -A and -I exhibit a stable binding pattern such as this, during their assembly in G1 and S-phase, respectively
Investigation of the assembly and inheritance of CENPs -T and -W reveals a dynamic pathway for assembly late in the cell cycle that is associated with rapid exchange of the proteins, such that they do not exhibit multigenerational persistence. As a group, centromere protein assembly occurs through several mechanistically distinct processes distributed throughout the cell cycle. The CENP-A assembly pathway itself is distributed from mid S-phase, when replication of CENP-A associated DNA occurs, through late G1 of the subsequent cell cycle, when RSF-dependent and MgcRacGap-dependent mechanisms stabilizes newly deposited CENP-A
The CENP-T/W complex has been shown to interact stably with histone H3 nucleosomes
Although the precise molecular organization of the histone H3-CENP-T/W nucleosome population is not known, it is reasonable to assume that they are interspersed closely with CENP-A nucleosomes, on the basis of known protein-protein interactions
The immediate requirement for CENP-W for successful execution of mitosis is consistent with the CENP-T/W complex playing an active role in kinetochore assembly in G2 and contrasts starkly with the ability of cells to accommodate loss of CENP-A over multiple generations without defect
The broadly used definitions of the centromere as a genetic locus, e.g. DNA, and the kinetochore as the facultative, proteinaceous structure on the primary constriction that executes mitotic function have lost distinction with the demonstration that a chromatin protein complex carries the genetic function of the locus. The different behaviour of the CENP-A and CENP-T/W chromatin compartments suggest a degree of segregation of genetic (centromere) function and mitotic (kinetochore) function within biochemically specialized chromatin microdomains of the centromere. Investigation of the roles of individual CCAN components in the maintenance of centromere identity, establishment of kinetochore function, and the integration of these two processes may provide important paradigms for understanding epigenetic chromatin-based inheritance.
Cells were cultured as previously described
pCLIPm (NEB) was adapted for use with the Gateway system (Invitrogen) by linearising the plasmid in the MCS by restriction digest with EcoRV. Gateway adaptation reading frame cassette RFB (Invitrogen) was ligated into the plasmid to generate a pCLIPm for C-terminal CLIP tagging (GW-pCLIPm). AttB flanked PCR products were generated from cDNA for CENP-T and CENP-W and recombined with pDonr-Zeo (Invitrogen) to generate entry vectors, which were recombined with GW-pCLIPm to generate the CENP-T-CLIP and CENP-W-CLIP constructs.
Cells stably expressing the CENP-A-SNAP fusion protein were a gift from L.E. Jansen. The CENP-A-SNAP cell line was transfected with the CENP-T-CLIP and CENP-W-CLIP constructs. Cells stably expressing the fusion proteins were selected by G418 (600 µg/ml; Calbiochem). The resulting monoclonal lines were expanded and examined by fluorescence microscopy after CLIP-505 labelling. CENP-T-CLIP 11 and CENP-W-CLIP 4 were used for all experiments in this paper. These lines exhibited population doubling times and cell cycle distributions indistinguishable from the parental line.
CLIP tag activity in cells was quenched by addition of 20 µM O6-BC (BC-block; NEB) in complete growth medium for 30 min at 37°C. SNAP or CLIP-tag proteins were pulse labelled with 2 µM CLIP-505/CLIP-Tmr Star/SNAP-Tmr Star (NEB) in complete growth medium supplemented with 1% BSA for 20 min at 37°C. After quenching or pulse labelling, cells were washed twice with prewarmed PBS and once with complete DMEM. Following washes cells were reincubated in complete medium to allow excess labelling compound to diffuse from cells. After 30 min, cells were washed again twice in PBS followed either by reincubation in complete DMEM, or fixation.
HeLa cells were treated with 5 mM thymidine in complete DMEM for 16 h, washed twice in PBS, once in complete DMEM, and released in complete DMEM 9 h followed by addition of thymidine to a final concentration of 5 mM for 16 h, after which cells were released again into complete DMEM.
Cells were grown and CLIP labelled on glass coverslips followed by fixation in MeOH or 4% PFA and processed for immunofluorescence. ACA (1∶500), Anti-CENP-A (a gift from K. Yoda, Nagoya University, Nagoya, Japan) was used at a dilution of 1∶200, human anti-PCNA serum was used at 1∶100, anti-α-tubulin (Sigma) was used at 1∶500, and anti-phospho histone H3 (Abcam) was used at 1∶300.
Donkey fluorescently conjugated secondary antibodies (anti-mouse-FITC (1∶50) anti-mouse Cy5 (1∶200), anti-rabbit TRITC (1∶100), and anti-human AMCA (1∶100) were obtained from Jackson Immunoresearch Laboratories. Samples were mounted in SloFADE (Invitrogen).
Images were captured using a DeltaVision Core system (Applied Precision) controlling an interline charge-coupled device camera (Coolsnap HQ2; Roper) mounted on an inverted microscope (IX-71; Olympus). For each sample, images were collected at 2× binning using a 100× oil objective at 0.2 µm z sections. All images were deconvolved and maximum intensity projected using SoftWoRx (Applied Precision). For quantification, unscaled DeltaVision images were used. Centromere signal intensity was determined using ImagePro 6.3. A mask was created using CENP-A or ACA signal to define centromeres. The "centromere" mask was applied to the CLIP-505 channel and the mean fluorescence intensity measured. A background mask was created from three regions within the nucleus not containing centromeres and applied to the CLIP-505 images for measurement. Background values were then subtracted from mean signal.
Whole cell extracts equivalent to 50,000 cells were separated by SDS-PAGE, transferred to PVDF membranes (Millipore), and processed for immunodetection as described previously
The authors thank Lars Jansen for the generous gift of HeLa SNAP-CENP-A cells and Kinya Yoda for CENP-A monoclonal antibody. KFS is a Research Professor of Science Foundation Ireland.
constitutive centromere associated network
fluorescence recovery after photobleaching
green fluorescent protein


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Top Ten Tips: Confident Counselling
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Counselling the 'missing step' in preventing suicide
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How to Support a Partner Struggling with Depression
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How to Deal With Unsupportive Friends and Family
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... am going more than 5 times to the bathroom. Thanks
Can you tell me the ingredients of RX Digex Nf Cap Pron? I had a prescription yesterday because I?
- Asked
- 7 Aug 2010 by garciah
- Active
- 23 Aug 2010
- Topics
- digex, neurofibromatosis
Responses (1)
The active ingredients in Digex NF are Hyoscyamine and Phenyltoloxamine.
It works by decreasing the motion of muscles in the stomach, intestines, and bladder. It also decreases the production of stomach acid.
It is used to relieve the symptoms of indigestion (eg, feeling of fullness, gas, bloating)and also to treat certain stomach and intestinal conditions, including spasms and cramps.
Read more: https://www.drugs.com/cdi/digex-nf.html#ixzz0xOJ9yWoC
It can cause urinary retention. Stop the medicine and if you have any problems see your doctor.
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Does anyone know if it is safe to split open a Digex NF capsule and take only half of it?
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I have a prescription of diphenhydramine cap 25mg that expired on 2/11, is it still usable?
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Mira lax dose from cap - white section of cap has line midway up to fuscia color?
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Optimal Search Strategies for Retrieving Scientifically Strong Studies of Treatment From Medline: Analytical Survey
Haynes, R. Brian
McKibbon, K. Ann
Wilczynski, Nancy L.
Walter, Stephen D.
Werre, Stephen R.
Bibliographic Citation
BMJ: British Medical Journal 2005 May 21; 330(7501): 1179-1182
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Optimal Search Strategies for Retrieving Scientifically Strong Studies of Diagnosis From Medline: Analytical Survey Haynes, R. Brian; Wilczynski, Nancy L. (2004-05-01)
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Author: TempleLaboratories
Norwescon schedule: panels, reading, teaching a workshop!
Lovers! If you're attending Norwescon this weekend, come check me out. I'll be there mainly Friday and Saturday, with kickoff party appearance Thursday night and a panel on Sunday. {click post title above for the rest}
Short notice gig: Reading from The PrEP Diaries, February 1, 2017
Hear ye, tricks. Chad Goller-Sojourner had to drop from the next Contagious Exchanges at Hugo House, Seattle, so I'll be filling in for him. Come hear never-before-read-in-public excerpts from The PrEP Diaries! Reading, Wednesday, Feb 1, 7PM: Contagious Exchanges: Queer Writers in Conversation. Reading excerpt of The PrEP Diaries with Julene Tripp Weaver and John… Continue Reading Short notice gig: Reading from The PrEP Diaries, February 1, 2017
Pirate Games
first published in At Second Glance: Gay City 4
Five Films Reviewed by Frankenstein's Creature
first published in Weird Tales # 359, edited by Ann VanderMeer
A Different Seed
first published in Glitterwolf Halloween 2014
Rejuvenate, Resurrect, Repopulate
Dear Loves, I've hand-waved away the old blog posts. The site is now undergoing a redesign in preparation to launch the new Evan J. Peterson: same great provocateur, now more focused and precise. Abundant thanks to the Clarion West writers workshop for accepting me and polishing me last year. My mentors, classmates, and admins turned… Continue Reading Rejuvenate, Resurrect, Repopulate
The Monster from Beyond Our World (of Literary Merit)
**Friends, here's a short essay that was previously published on the Richard Hugo House website. It was a promotional item for my RHH class, Writing the Monster. I'm reposting here now that it's unavailable elsewhere.**


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Why so much hate for Supebot?
They didn't announced on a event, and you guys are jumping to conclusion that they sucj, they are bad when b4 the event you were loving them for their hard work. Go back to past and start doing that again because all this hate ain't going to help. Just be calm and try to contact them and no just argue about their bad work at the event. Maybe they will release something online, how much time is there left on the event?
Re: Why so much hate for Supebot?
The only reason why we are dissapointed is beacause Omar Kendall himself said that there would be reveals, if not then we wouldve been fine, but ppl dont like to get lied on
Re: Why so much hate for Supebot?
Re: Why so much hate for Supebot?
Re: Why so much hate for Supebot?
Yeah I pretty much lost all my respect for Superbot. they don't understand the fan support for this game. If they don't reveal at Eurogamer, which as of right now they do not have a panel. then I may have lost interest in this game.


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Zayn Malik 's mum has spoken out to reassure fans her son is "fine" after he announced he'd be pulling out of the One Direction tour due to stress.
The singer pulled out of a commercial recording in Hong Kong today so he could fly back to the UK to be with his family.
A spokesperson for the band said: "Zayn has been signed off with stress and is flying back to the UK to recuperate. The band wish him well and will continue with their performances in Manila and Jakarta."
Now Zayn's worried mum Trisha says she has been in constant contact with her son by phone.
Speaking at her detached home in Bradford, West Yorkshire, she said: "I have spoken to him and he's fine. We have been in contact.
"I cant say where he is going to go."
This comes after the 22-year-old hit out at "jealous f***s" after pictures emerged of him with a mystery girl.
Last night he wrote on Twitter: "I'm 22 years old... I love a girl named Perrie Edwards. And there's a lot of jealous f***s in this world I'm sorry for what it looks like x."
The rumour mill went into overdrive after pictures were shared of the engaged star holding hands with a blonde girl named Lauren outside Seduction nightclub in Phuket, while they were recently there for a gig.
They were later followed by another shot of the singer appearing to hug the mystery woman, with his arms wrapped around her shoulders.
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- Watch Louis Tomlinson on America's Got Talent0:47


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SVP of A&R, RCA RECORDS
Katie Welle is SVP of A&R for RCA Records, heading up the labels West Coast A&R department. She oversees a number of artist projects through signing, recording and release planning - liaising with artists, their managers, attorneys, songwriters and producers. RCA Records is one of the worlds most successful record labels, home to Justin Timberlake, the Foo Fighters, Usher, Miley Cyrus, Kelly Clarkson and A$AP Rocky to name a few.
Welle came to RCA after 7 years at Sony/ATV Publishing where she served most recently as VP of Creative. There she was responsible to a number of successful artist and songwriter campaigns such as Jeff Bhasker, Mikky Ekko, the Cataracs and Odd Future. She is still aligned with Sony/ATV as a consultant. Prior to that Welle worked at Epic Records in A&R.
Tuesday, March 14, 2017 at 12:34PM | Elysa
Last night, CBS Los Angeles came out to Venice Arts for a story on how the proposed cuts to the federal budgetincluding the elimination of ...
Wednesday, February 15, 2017 at 1:45PM | Elysa
We are thrilled to announce that Voices of the Forest: Thailand will be featured at the Earth Optimism Summit at the Smithsonian in Washingt ...
Monday, February 06, 2017 at 11:07AM | Elysa
On Friday, some of our talented Advanced Filmmaking students had the opportunity to take a most extraordinary site visit to Adaptive Studios ...
Tuesday, January 17, 2017 at 12:45PM | Elysa
For the fourth year in a row, Venice Arts will participate in the International Los Angeles Photographic Art Exposition photo l.a., with an ...
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Good investment potential offered by this 4 unit in the heart of Sellersville. Strong rental history. Tenants pay heat and electric. Landlord pays water/sewer & trash. Potential for higher rents if the units are remodeled. Minimum 24 hour notice needed for all showings.