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The FT doesn't usually publish total nonsense, but there's a story today which made me choke on my cup of tea.
According to a new study by the British Council, China is supposed to have more English speakers than India.
这篇文章的作者大卫·葛拉多尔(David Graddol)认为印度懂英语的人数占总人口5%,而中国每年新增2000万懂英语的人。
David Graddol, the author, puts the number of English speakers in India at five per cent, or 55 million, and says China is turning out 20 million new English speakers each year.
The FT says if this is true, India has lost one of its "clear economic advantages" over China and will find it harder to compete, given China has superior infrastructure and a more flexible supply of labour.
Anyone who has been to both countries, however, can see that this survey is bogus. Barely anyone in China speaks English.
By contrast, almost every Indian has a basic English vocabulary. And the Indian middle class, the politicians, businessmen and intellectuals, are all fluent.
Students in China are forced to rote-learn English in school, sometimes for years, but it is a rare person who can actually speak the language. Even the foreign sales managers of export businesses, who depend on business from American and English companies, are often oddly tongue-tied.
It is easier to rub by in Beijing and Shanghai, which are both fairly touristy, but simple things, like taking a taxi, remain nearly impossible without a bit of Mandarin. And I always worry about the tourists who backpack around China. If they cannot speak Chinese, I imagine they have an intensely lonely, difficult and unpleasant time.
China, because of its size and its centuries of isolation, rivals the US and the UK as a nation of monoglots.
Unlike the US and the UK, however, China does have a grand ambition to transform itself, and mastering English is part of the plan. There is a great piece here, from the New Yorker, about the drills in English before the Olympics last year.
同时,我有一个中国人学习英语的精彩故事,故事源于张墀言(Leslie Chang)的书《工厂女工》。在中国东莞的"装配线学习机器"学校,学生每月交600元钱,坐在一椭圆的机器前。盯着机器看,机器上会闪过一系列英语单词。
Meanwhile, my favourite story about learning English comes from Factory Girls, by Leslie Chang. At the "Assembly Line Learning Machines" school in Dongguan, one of China's biggest factory cities, students paid 600 yuan (£52) a month to sit in front of oval metal machines. As the students look at the machines, they rotate a series of words past their eyes.
Mrs Chang writes: "A column of words floated past me. CLEAN, RUDE, PIZZA, CREEP."
She explains: "The guiding principle was that treating people like machines was the key to mastering English. After learning the alphabet and the phonetic sounds of the language, a student sat at a machine while columns of English words rotated past. The student read aloud each word and wrote it down without knowing what it meant, week after week, until he attained the highest speed. He then proceeded to another machine that showed Chinese definitions of words; next he advanced to short sentences. When a student achieved the top speed – able to write six hundred English sentences in one hour – he graduated to basic grammar. Only then did he learn the meaning of the words, phrases and sentences he had been repeating for months."
She goes on to speak to the inventor, Mr Wu. "'On the assembly line, people can sit and work for eight or ten hours without rest,' he said. 'If only we could learn that way, how good it would be!'". Needless to say, Mr Wu didn't prosper.
2009-11-22 20:14 编辑:kuaileyingyu
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Combtail Betta Fish – Interesting Facts You Might Not Know
There are many types of betta splendens out there and the amazing thing about them is they are all very interesting. Their tails and colors all look beautiful, which is why they are a huge favorite among fish tank enthusiasts. The Combtail betta fish is no exception. This is probably one of the most difficult types of fish to identify since it could easily be confused with a Crowntail.
How To Identify The Combtail Betta
Here are some interesting traits that could help identify a Combtail betta fish:
- First and foremost, the main trait that helps to identify it is that the edge of the fins looks almost like a comb.
- Its tail spreads as wide as that as a Halfmoon but with a lot less smoother edges. There are some Halfmoons, however, that have fringes, but if it looks like fur rather than a more pronounced comb, then it is not a Combtail.
- It looks like a Crowntail but with less extended spikes, fringes, or rays.
- The splits between each ray should be drastic enough to be seen from afar. The splits should be at least a third from where it begins all the way to the tip, whereas a Crowntail's splits are so drastic that it covers the outer half of its fins.
Other pictures that might help you identify it:
Interesting facts about the Combtail Betta Fish
- The Combtail is also referred to as the Half Sun betta.
- It is quite questionable as to how the Combtail came to be. It is believed to have been bred from a Crowntail and another type of Betta, hence, the more subtle tail edge. Others say it is indeed a bred type but that the Crowntail was actually an improved version of it. Of course there are some who think that the rays that extend past the edges of its fins are naturally occurring.
- The rays of the Combtail's fins also come in a variety of shapes. There are some that seem to split at the tip, like a fork. There are also the more common shapes like pointed and rounded.
Combtail Betta Fish In Action
Before I forget, here's a short video giving you a better idea on what the combtail betta fish looks like:
Do you know anything else useful or fun about the combtail betta fish that you think I should mention? Or other fun betta fish facts? If so, please comment on my post!
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Forex: AUD/USD: Aussie strength highlights 0.8810 - FXTechstrategy
FXstreet.com (Barcelona) - The Australian Dollar remains consolidating above 0.8700 after having broken through 0.8675 to hit a fresh 12 month high at 0.8730, and according to Mohammed Isah, technical analyst at FXTechstrategy, targeting 0.8810.
Isah sees the Aussie strong enough to continue uptrend towards Aug 08 high at 0.8810: More strength continues to be seen as AUDUSD was seen breaking above its brief consolidation high at 0.8674 in early trading today targeting the 0.8810 level, its Aug 17'08 high. Its broader medium term uptrend triggered off the 0.6246 level in Feb'09 remains on course as a break of the latter level will put the pair on the path to further upside gains towards the 0.8950 level, its Aug 10'08 high.
On the downside Isah sees initial support at 0.8674 level, the Sept 11'09 high: Downside objectives are located at the 0.8674 level, the Sept 11'09 high and then the 0.8543 level, its Sept 14'09 low. Others are located at the 0.8476 level, its Aug 14'09 high and its Jun'09 high at 0.8262.


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Do Grandparents Have Visitation Rights in New York?
Learn what judges consider when making decisions about grandparent visitation in New York.
Are you concerned about how a divorce, separation, or parent's death will interfere with your relationship with your grandchildren? These life-altering changes in the family can impact the grandparent-grandchild relationship, especially when a surviving or custodial parent tries to limit your time together. This article explains when and how grandparents can request court-ordered visitation with their grandchildren in New York.
Do I have a legal right to grandparent visitation?
In New York, grandparents have a legal right to request court-ordered visitation when:
- one or both parents die
- they have a substantial existing relationship with their grandchildren, or
- the child's parents have interfered with their efforts to establish or maintain a relationship.
This right applies only to biological or adoptive grandparents and does not extend to great-grandparents or other relatives.
What must I prove in court?
The grandparent asking for visitation has the "burden of proof" (the duty to provide sufficient evidence) to show a legal right to court-ordered time and that the proposed visits are in the child's best interests.
First, you must establish legal grounds for your request. If one or both parents die, this determination is automatic. If one or both parents are living, you must prove an existing relationship with your grandchild or demonstrate that the child's parent(s) have prevented you from establishing one.
Judges must give great weight to parental preferences regarding who gets to visit their children, balancing the parents' objections with other factors, such as the current family structure and the extent of any existing relationship. Evidence of animosity between a grandparent and parent(s) is relevant but not enough, alone, to deny a request for court-ordered time.
Once you have established legal grounds to request visits, you must show that spending time with you is in your grandchild's best interest, considering:
- the child's age
- the child's wishes, if he or she is mature enough to express a preference
- the distance between the child's home and where your proposed visits will occur
- the mental and physical health of everyone involved, including the child, his or her parents, and anyone else that may have filed for visitation or custody
- your past and current relationship with the child and parent(s), and/or
- your nurturing skills and attitude towards the parent(s).
The court will assign an attorney to represent the child's interests who is specially trained to present his or her wishes or speak in his or her best interests.
How do I start the process?
You must file a "petition," (formal written request) with the court in the county where your grandchild lives. In your petition, you will describe your proposed schedule for court-ordered time. Once you've filed your request, you must give notice to everyone involved, including the child's parents and anyone else that may have filed for custody.
If you already have a visitation order, but you want more time or the child's parent is preventing you from visiting, you can ask the court to "modify" (change) it, or enforce the order.
Do I have rights if my grandchild is adopted?
In New York, adoption does not cut off a biological grandparent's rights to request visitation. The primary consideration is the child's best interest, considering health, safety, and welfare, and whether your visitation request is made before, during, or after an adoption.
How do I get custody or guardianship of my grandchild?
New York courts will only consider awarding custody to a non-parent if the natural parent abandons or severely neglects the child, and the non-parent proves it would be in the child's best interests.
If you have questions about your right to spend time with your grandchild, you should speak to a local, experienced family law attorney, who can help you navigate this process.
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Background Sufferers with familial adenomatous polyposis (FAP) are recognized to have an increased risk for gastric adenomas. and in the torso not connected with FGP (one individual). Adenoma size was 3-40 mm and the real variety of adenomas per individual ranged in one to 20. Adenomas in the antrum had been flat and simple whereas those in the gastric body or fundus had been polypoid and tough to differentiate in the cystic FGPs observed in sufferers with FAP. The executing endoscopists reported problems with determining adenomas and six sufferers acquired at least one EGD within the prior 3 years where gastric adenomas weren't reported. Adenomas had been categorized as tubular in eight sufferers and tubulovillous in a single individual. High quality dysplasia was observed in one individual. After a median follow-up of 63 a few months (interquartile range: 20-149 a few months) no individual in our whole cohort (with or without gastric adenomas) created gastric cancers. The sufferers in whom gastric adenoma made in comparison to those without gastric adenoma had been more likely to become youthful [36 ± 12 vs. 48 ± 15 years p = 0.02] possess concomitant chronic gastritis [22% vs. 0% p = 0.008] and also have desmoid tumors [5 (56%) vs. 19 (22%) p = 0.04]. AS 602801 Conclusions Gastric adenomas aren't uncommon in sufferers with FAP and so are often difficult to recognize endoscopically. Endoscopists must have a high amount of suspicion for gastric adenomas in these sufferers and a minimal threshold to biopsy. Provided the benign scientific course recommended preliminary management is conventional with endoscopic therapy and regular security. gene mutation examining. The scholarly study was approved by the Mayo Medical clinic Institutional AS 602801 Review Plank. For each individual data was gathered including age group gender genealogy of gastric cancers the current presence of cystic fundic gland polyps (FGP) gastric adenomas and duodenal adenomas with intensity staged using the improved Spigelman classification [7] the current presence of desmoid tumors cigarette smoking history and usage of nonsteroidal anti-inflammatory medications (NSAID) and/or proton pump inhibitors (PPI). In sufferers with gastric adenomas we retrieved data on endoscopic and histological features kind of directed therapy procedure-related problems development of cancers and amount of follow-up. Statistical analyses The Pearson chi-square check or Fisher specific check was utilized to measure the univariate organizations AS 602801 between a categorical adjustable and the current presence of gastric adenoma. The training student t test was employed for age. A two-tailed p-value was found in all of the analyses. A p-value?0.05 was considered significant statistically. All data were analyzed using SPSS Statistics software (version 16; IBM Corporation USA). Results Nine of 97 individuals with FAP (9.2%) had biopsy-proven gastric adenomas. Mean age at the time of diagnosis having a gastric adenoma was 36 years (range 18-52 years). The median follow-up for the entire cohort was 63 weeks (interquartile range: AS 602801 20-149 weeks). High-grade dysplasia (HGD) was found in one patient (Patient 7) at their initial analysis of gastric adenoma (Table? 1 Table 1 Clinical characteristics of familial adenomatous polyposis individuals with gastric adenomas Of the nine individuals with gastric adenomas four experienced adenomas located in the gastric antrum three experienced adenomatous polyps associated with FGP one experienced adenomas in the gastric body without underlying FGP and one patient experienced adenomas in the gastric antrum and the gastric cardia. Gastric adenomas ranged in size from 3-40 mm and the number of adenomas ranged from one to 20 per individual. Adenomas in the antrum were smooth sessile and delicate having a villiform reddish appearance whereas those in gastric body or fundus were polypoid lobular with pale yellow surface and hard to differentiate from your benign FGP seen in individuals with FAP (Numbers? 1 and ?and2).2). A majority of carrying MGC3199 out endoscopists reported the adenomas were difficult to identify. Six individuals experienced at least one EGD within the previous three years without reported endoscopic results recommending adenomas. Histology was tubular in eight sufferers and tubulovillous in a single individual. None from the nine sufferers with gastric adenoma acquired Helicobacter pylori. Amount 1 Individual 5. (A) Many little sessile tubular adenomas in the gastric antrum of the 39-year-old male individual with AS 602801 familial adenomatous polyposis (arrows). The adenomas possess a flat-topped villiform appearance. (B) A photomicrograph of the gastric adenoma from … Amount 2 Individual 2. (A) A.
Matrix Metalloproteinase (MMP)
Activation of EphB receptors by efnB ligands on neuronal cell surface
Activation of EphB receptors by efnB ligands on neuronal cell surface regulates important features including neurite outgrowth axonal assistance and synaptic plasticity. capability of brain-derived neurotrophic aspect (BDNF) to safeguard Daphnetin neuronal civilizations from glutamate-induced cell loss of life depends upon PS1. Neuroprotective functions of both efnB and BDNF were indie of γ-secretase activity however. Lack of PS1 reduces cell surface appearance of neuronal TrkB and EphB2 without impacting total cellular degrees of the receptors. Furthermore PS1 knockout neurons present defective ligand-dependent internalization and decreased ligand-induced degradation of Eph and TrkB receptors. Our data present that PS1 Octreotide mediates the neuroprotective actions of efnB and BDNF against excitotoxicity and regulates surface area appearance and ligand-induced fat burning capacity of their cognate receptors. Together our observations show that Daphnetin PS1 promotes neuronal survival by regulating neuroprotective functions of ligand-receptor systems. were performed against the value of untreated basal condition (* p < 0.05; ** p < 0.01; ***p < 0.005). 3 Results 3.1 EfnB1 protects neurons from excitotoxic cell death EphB receptors are activated by efnB ligands and interact with PS1 a protein involved in neurodegeneration (Litterst et al. 2007 To examine whether the efnB/EphB system affects neuronal survival we used glutamate-treated mature rat cortical neuronal cultures of 8-13 DIV and efnB1-Fc recombinant constructs. These contain the extracellular domain name of mouse efnB1 fused to Fc and are used as ligands able to bind and activate EphB receptors mimicking the iligand effects (Davis et Daphnetin al. 1994 Litterst et al. 2007 Physique 1A shows that treatment of our cultures with efnB1-Fc rescues neurons from glutamate-induced cell death measured by counting healthy nuclei stained with Hoechst stain (observe Methods). Fc alone or N-cadherin-Fc made up of the extracellular domain name of N-cadherin fused to Fc experienced no effect on neuronal survival (data not shown). The neuroprotective function of efnB1 against excitotoxicity Daphnetin was manifested in all mature cortical neuronal cultures 8 to 13 DIV. EfnB1-Fc rescues neurons from glutamate toxicity when it is added to cultures before or at the same time as glutamate but it is normally inactive if added afterwards than thirty minutes pursuing glutamate treatment (Fig. 1B). Dose-response tests demonstrated that neuroprotection depends upon the focus of efnB1-Fc using a fifty percent maximum aftereffect of about 0.5μg/ml (Fig. 1C). This neuroprotective impact is also discovered by the power of cells to lessen 3-(4 5 5 bromide (MTT) to formazan (Morgan 1998 Xu et al. 2011 an assay of mitochondrial activity (Amount 1D) and by calculating released lactate dehydrogenase a trusted assay of cytotoxicity (Amount 1E). The efnB1 neuroprotection against glutamate was noticeable by calculating neuronal procedures using microtubule linked proteins (MAP2) immunostaining. Glutamate decreases the amount of neuritic extensions per cell an final result partly reversed by efnB1-Fc aswell as by neurotrophin BDNF (Figs. 2A and 2B). This aftereffect of efnB1 in post-mitotic cortical neurons is normally noteworthy since it shows that efnB preserves the integrity of neuronal procedures and may have got Daphnetin neurotrophic features. Cell staining with transfected green fluorescence proteins (GFP) enables morphological id of making it through neurons (Amount 2C). These tests showed which the kinetics of neuronal success of efnB-treated neurons is related to the kinetics of BDNF-dependent success (Amount 2D). Usage of Daphnetin efnB2-Fc constructs filled with the extracellular series of efnB2 another person in the efnB category of ligands that binds EphB receptors and it is extremely homologous to efnB1 provided results identical to people attained with efnB1-Fc (data not really shown) Amount 1 EfnB1 protects neurons from excitotoxic insults Amount 2 Neuroprotective activity of efnB1 by MAP2 staining and time-dependent morphological adjustments 3.2 EphB2 receptor mediates the efnB1-reliant neuroprotection EfnB1 ligands exert their neuronal features by binding neuronal EphB receptors including EphB1 EphB2 and EphB3 (Lackmann and Boyd 2008 To examine if the neuroprotective ramifications of efnB are mediated by.
The voltage-gated sodium channel (Nav) 1. of Nav1.8 reduced their surface
The voltage-gated sodium channel (Nav) 1. of Nav1.8 reduced their surface expression. Alanine-scanning analysis revealed acidic amino acids as critical factors in the unusual transmembrane sections. Furthermore co-immunoprecipitation tests demonstrated that calnexin interacted with acidic amino acid-containing sequences through its transmembrane portion. Overexpression of calnexin led to increased degradation of these protein through the ER-associated degradation pathway whereas down-regulation of calnexin reversed the phenotype. Hence our benefits reveal a crucial mechanism and function of transmembrane segments in surface expression and degradation of Nav1.8. check. Co-immunoprecipitation HEK293 cells had been lysed in recovery buffer (1% Triton X-100 50 mm Tris-HCl pH 7.5 and 150 mm NaCl) as well as the lysate examples were incubated overnight at 4 °C with each antibody as described in the figure legends accompanied by incubation with proteins G-Sepharose beads (Amersham Biosciences) for 2 h at 4 °C. The immunoprecipitates were washed with A 922500 recovery buffer and analyzed by Western blotting efficiently. The test was repeated at least 3 x. Immunocytochemistry The transfected COS-7 cells expanded in the cup coverslips had been set with 4% paraformaldehyde at 4 °C for 15 min. For co-localization staining of FLAG-TFR1(TMIVS3)-Myc using the ER marker calnexin COS-7 cells had been incubated right away with mouse antibody against Myc (1:500) and rabbit antibody against calnexin (1:1000) at 4 °C. The cells A 922500 had been incubated Rabbit Polyclonal to PARP4. with an assortment of donkey antibody against mouse conjugated with FITC (1:100; Jackson ImmunoResearch Western world Grove PA) and donkey antibody against rabbit conjugated with Cy3 (1:100; Jackson ImmunoResearch). For non-permeabilized staining of surface area protein live COS-7 cells transfected with FLAG-TFR1-Myc chimeric protein or Myc-CD8α chimeric protein had been first tagged with mouse antibody against Myc (1:100) diluted in Ca2+/Mg2+ PBS for 1 h at 4 °C. Then your donkey antibody against mouse conjugated with FITC was incubated for 30 min at 4 °C. For permeabilized staining of total protein the cells had been set and incubated with rabbit antibody against Myc (1:500 in 0.3% Triton X-100; Sigma) right away at 4 °C. The donkey antibody against rabbit conjugated with Cy3 was incubated for 45 min at 37 °C. Finally the cells had been examined utilizing a ×63 essential oil zoom lens (numerical aperture 1.32) using a Leica DMRE microscope and pictures were captured using the SP2 laser beam scanning confocal program in ~20 °C (Leica Germany). The immunofluorescence intensities of surface area and total proteins had been quantified by Image-Pro Plus software program as well as the statistical outcomes had been examined using Sigma Story 10.0 predicated on at least 45 cells from three individual tests. All data had been shown as suggest ± S.E. and examined by matched Student's test. Outcomes The Transmembrane Sections of Nav1.8 Prevent Surface Appearance of Chimeric Protein Nav1.8 includes four homologous domains termed I-IV. Within each area you can find six transmembrane sections known as S1-S6 (Fig. 1and ?and and and22and and and and … To investigate the impact of ER-localization proteins in the transmembrane sections on the top appearance of full-length Nav1.8 we performed alanine substitution for every ER-localization amino motifs or acids to get five mutants including Nav1.8IIIS3-D1223A-GFP Nav1.8IVS1-D1480A-GFP Nav1.8IVS3-D1544A-GFP Nav1.8IIS1-D663A/P644A/E667A-GFP and Nav1.8IIS3-N729A/I730A/D732A-GFP. We portrayed Nav1.8-GFP or its mutants to HEK293 cells due to the bigger transfection efficiency within this cell line compared to the COS-7 cell line. Two times after transfection cell-surface biotinylation and immunoblotting demonstrated the fact that mutated channels triggered a 3-4-flip increase in surface area expression compared to the wild-type Nav1.8 (Fig. 5 and and and and F). Our data claim that the transmembrane portion of calnexin interacts using the acidic amino acid-containing transmembrane portion of Nav1.8. To research A 922500 the function of calnexin in the degradation of protein containing acidic proteins in the transmembrane A 922500 portion we overexpressed calnexin in HEK293 cells stably expressing FLAG-TFR1(TMIVS3)-Myc or Nav1.8-GFP. We noticed the fact that steady-state degrees of both protein had A 922500 been reduced by calnexin overexpression however not calnexin(ΔC) (Fig. 7G) a.
To investigate whether the addition of antibiotic/antimycotic during individual granulosa-lutein cells
To investigate whether the addition of antibiotic/antimycotic during individual granulosa-lutein cells (GLCs) isolation and cell-plating techniques prevents microbial contaminants after 144?h of lifestyle and in addition evaluate the ramifications of contaminants on GLCs steroid and ultrastructure secretion. isolated with just PBS. Infections disrupted the adherence from the GLCs towards the lifestyle dish interfering with monolayer development affecting the development design of GLCs. Several mobile debris and bacteria were observed and no organelles were found in the cytoplasm of infected Irbesartan (Avapro) cells. While bacterial contamination decreased estradiol press levels it improved progesterone as compared with noncontaminated group. Taken collectively our data showed the addition of a high dose of antibiotic/antimycotic during the isolation and cell-plating methods prevents microbial contamination of long-term GLCs tradition as its effects on cells growth and function maturation (IVM) like a potential strategy to safeguard fertility in malignancy individuals [1 2 offers increased the attention of the reproductive biology researches in controlling long-term ovarian cells tradition. Studies using human being granulosa-lutein cells (GLCs) aspirated during oocyte retrieval for fertilization (IVF) process may ultimately provide data to further development of adequate milieu for oocyte growth and maturation by coculture of these cells with immature oocytes or preantral follicles. Furthermore physical and metabolic integrity of oocyte and surrounding granulosa cells connection is essential for the normal course of the folliculogenesis process [3-5] which makes the managing of the varieties Irbesartan (Avapro) which frequently reside in the vagina [9] and even by additional normal vaginal flora like lactobacillus [10]. As bacterial endotoxins dramatically disrupt granulosa cells growth and function in different varieties [11-13] a technique for the isolation and plating of human being GLCs that prevent microbial contamination would provide a powerful tool to ensure the long-term administration of useful cells check. < 0.05 was considered significant statistically. 3 Outcomes 3.1 Morphology of Individual Granulosa-Lutein Cells Is Preserved after Long-Term Lifestyle Human GLCs gathered with PBS containing antibiotic/antimycotic had been clear of bacterial contaminations after 144?h of lifestyle. Noninfected GLCs demonstrated their usual elongated morphology of adherent cell that increases in monolayer (Amount 1). The ultrastructural analyses demonstrated nucleoplasm included finely dispersed chromatin and peripheral areas of heterochromatin (Amount 1(a)) being a thick cytoplasm contained many organelles and apparent connections between cells (Amount 1(b)). Individual GLCs collected just with PBS demonstrated bacterial contaminations after 144?h in lifestyle (Amount 2). Ultrastructural analyses demonstrated disrupted GLCs cytoplasm filled with innumerous vacuoles and bacterias (Statistics 2(a) and 2(b)) as undefined nucleus (Statistics 2(c) and 2(d)). Many bacteria had been observed in the cytoplasm and in the extracellular mass media Ntf3 next towards the cells or the cell particles (Amount 2(c)). Before plating the cell viability was evaluated by trypan blue dye exclusion and it had been 90% (data not really shown). Amount 1 Transmitting electron micrographs of individual Irbesartan (Avapro) granulosa-lutein cells (GLCs) gathered with PBS filled with antibiotic/antimycotic cultured for 144?hs in TCM-199 moderate. (a) Usual elongated wellness granulosa cell displaying cytoplasm with organelles … Amount 2 Transmitting electron micrographs of individual granulosa-lutein cells (GLCs) collected only with PBS cultured for 144?hs in TCM-199 medium. (a) Disrupted GLCs cytoplasm comprising innumerous vacuoles and several bacteria. (b) Details of bacteria cytoplasm … 3.2 Effect of Bacterial Contamination on Human being Granulosa-Lutein Cells Steroid Production Estradiol production by GLCs that were collected with PBS Irbesartan (Avapro) supplemented with antibiotic/antimycotic was higher than that produced by cells collected only with PBS (Number 3(a)). In contrast the progesterone production by GLCs cells collected only with PBS was higher than that produced by cells that were previously washed in PBS supplemented with antibiotic/antimycotic (Number 3(b)). Number 3 Effect of bacterial contamination on human being granulosa-lutein cells (GLCs) steroid secretion. Concentrations of press estradiol (a) and progesterone (b) after 144 hours of tradition. GLCs collected with PBS comprising antibiotic/antimycotic (noninfected group) … 4 Conversation In this statement we explained an Irbesartan (Avapro) optimized protocol for isolation of human being granulosa-lutein cells where the cells were rinsed in PBS comprising antibiotic/antimycotic prior to the tradition. Although antibiotics are regularly added to press for.
Purpose of review This review summarizes recent metabolomics studies of renal
Purpose of review This review summarizes recent metabolomics studies of renal disease outlining some of the limitations of the literature to date. in diabetic nephropathy and a preference for aerobic glucose metabolism in PKD; in each case these studies have outlined novel therapeutic opportunities. Finally as a complement to the longstanding interest in renal metabolite clearance the microbiome has been increasingly recognized as the source of many plasma metabolites including some with potential functional relevance to CKD and its complications. Summary The high-throughput high-resolution phenotyping enabled by metabolomics technologies has begun to provide insight on renal disease in clinical physiologic and experimental contexts. analyses of ~100s of metabolites of established identity. By contrast methods that measure ~1000s of metabolite peaks (only a subset Rabbit Polyclonal to Collagen I. of which have assigned identities) generally utilize time-of-flight and ion trap mass spectrometers. Instead of monitoring pre-specified precursor ion-product ion pairs these instruments leverage their superior mass accuracy relative to triple quadrupole instruments to facilitate metabolite identification with current instruments providing m/z resolution to the fourth decimal place. Whereas the majority of renal metabolomics studies to date have applied NMR or MS-based methods increasing interest is being directed towards MS-based approaches in parallel with efforts to assign unambigious identities to many of the resulting unknown analyte peaks. Figure 1 Overview of Metabolomics Technologies CLINICAL STUDIES Given long-standing interest in small molecules as uremic toxins initial applications of metabolomics in nephrology research examined plasma or dialysate from individuals with ESRD [12-15]. Although these studies generated a broad view of the metabolite alterations that accompany Bleomycin ESRD they were unable to identify the alterations of greatest biologic or clinical significance. First because of the advanced and widespread physiologic derangements in ESRD these studies could not disentangle the relative Bleomycin contributions of decreased urinary clearance hemodialysis underlying comorbidities impaired nutrition changes in the microbiome etc. on the metabolome. Second the cross-sectional nature of these studies did not permit association of select metabolite alterations with longitudinal outcomes of interest. Recent studies have begun to address some of these limitations. Cross-sectional studies Metabolomic surveys of earlier stages of CKD have provided insight on how metabolite alterations vary across levels of kidney function [16-19]. Bleomycin Duranton used a commercial LC-MS metabolomics vendor to measure amino acids and amino acid derivatives in plasma and urine from 52 individuals across different stages of CKD and plasma only from 25 individuals on dialysis [20]. By examining paired plasma and urine they were able to determine that uremic elevations in plasma ADMA are attributable to decreased urinary clearance whereas elevations in plasma citrulline are due to overproduction. Posada-Ayala used NMR based discovery and LC-MS based validation to demonstrate that a panel of seven urinary metabolites could distinguish 31 individuals with CKD from 30 individuals without CKD [21]. Although plasma samples were not examined in this study the finding of elevated urinary levels of trimethylamine-N-oxide (TMAO) guanidoacetate and phenylacetylglutamine in CKD subjects suggests that these established uremic retention solutes are overproduced in CKD. Longitudinal studies Because early markers may provide more clinical and biologic insight than changes that occur in later stages of disease recent studies have examined whether baseline metabolite profiles are associated with future CKD or CKD progression. Yu used a commercial LC-MS/GC-MS based platform to measure 204 metabolites in plasma from 1921 African-American participants of the Atherosclerosis Risk in Communities study [22]. The authors found that lower levels Bleomycin of 5-oxoproline and 1 5 were associated with new onset CKD as defined by an eGFR<60 mL/min per 1.73 m2 and <75% of baseline or a CKD-related hospitalization or death. The authors speculate that higher levels of 5-oxoproline may report on increased glutathione.
Clinical pain conditions may remain attentive to opiate analgesics for prolonged
Clinical pain conditions may remain attentive to opiate analgesics for prolonged periods AM679 but such continual acute agony can undergo a transition for an opiate-resistant persistent pain declare that becomes a much more severe clinical problem. μ-opioid agonist DAMGO to produce tolerance for its inhibition of prostaglandin E2 (PGE2) hyperalgesia simultaneously produced hyperalgesic priming. Conversely injection of an inflammogen carrageenan used to produce priming produced DAMGO tolerance. Both effects were prevented by inhibition of protein kinase Cε (PKCε). Carrageenan also induced opioid dependence manifest as μ-opioid receptor antagonist (CTOP)-induced hyperalgesia that like priming was PKCε- and Gi-dependent. These findings suggest that the transition from acute to chronic pain and development of μ-opioid receptor tolerance and dependence may be linked by common cellular mechanisms in the primary afferent. All AM679 screening was carried out between 10:00 am and 4:00 pm. Experimental protocols approved by the University or college of California San AM679 Francisco Committee on Animal Research conformed to National Institutes of Health Guidelines for the Care and Use of Laboratory Animals. Nociceptive screening The nociceptive flexion reflex was quantified with a Basile Analgesymeter (Stoelting Chicago IL) which applies a linearly increasing mechanical pressure to the dorsum of a rat's hind paw. Nociceptive threshold Rabbit polyclonal to IKK-gamma.Familial incontinentia pigmenti (IP) is a genodermatosis that segregates as an X-linked dominant disorder and is usually lethal prenatally in males (The International Incontinentia Pigmenti Consortium, 2000 [PubMed 10839543]).In affected females it cause. defined as the pressure in grams at which the rat AM679 withdraws its paw is the mean of 3 readings taken at 5-min intervals. For nociceptive screening rats were placed in cylindrical transparent restrainers designed to provide adequate comfort and ease and ventilation allow extension of the hind lower leg from your cylinder and minimize stress. All rats were acclimatized to the screening process. Each paw was treated as an independent measure and each experiment performed on a separate group of rats. The results are expressed as percentage change from baseline mechanical nociceptive threshold decided before administration of test agent. Drugs and their administration Drugs employed in this study were prostaglandin E2 (PGE2; a hyperalgesic agent that directly sensitizes nociceptors) γ carrageenan (CARR inflammogen) and pertussis toxin (PTX a selective inhibitor of Gi-proteins) from Sigma (St. Louis MO); [D-Ala2 N-MePhe4 Gly-ol]-enkephalin (DAMGO) AM679 (a μ-opioid receptor agonist) from Research Biochemicals (Natick MA) pseudo receptor octapeptide for activated PKCε (ψεRACK; a specific agonist of PKCε) from SynPep Corp. (Dublin CA) D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr-NH2 (CTOP) a potent and highly selective μ-opioid receptor antagonist (Tocris Bioscience Ellisville MO) and EAVSLKPT (PKCεV1-2 a selective PKCε translocation inhibitor peptide EMD Bioscience La Jolla CA). The selection of the drug doses used in this study was based on dose-response curves decided in previously published studies (Aley and Levine 1997 Aley et al. 2000 Liu and Anand 2001 Joseph and Levine 2004 Joseph et al. 2004 Joseph et al. 2008 The stock answer of PGE2 (10 μg/μl) was prepared in ethanol and further dilutions manufactured in saline yielding your final ethanol focus of significantly less than 1%. All the drugs had been dissolved in saline. All medications administered intradermally had been in a level of 5 μl utilizing a 30-measure hypodermic needle mounted on a 10-μl Hamilton syringe except carrageenan which due to its high viscosity was injected utilizing a 27-measure needle. When an antagonist was included it had been injected either 30 min before the agonist or co-injected using the agonist. When medication combinations had been co-injected these were administered in the same syringe so the medication listed initial reached the intradermal site initial. Antisense and mismatch oligodeoxynucleotide Oligodeoxynucleotide (ODN) antisense and mismatch to PKCε had been prepared as defined previously (Parada et al. 2003 Dina et al. 2006 The antisense ODN 5 AGC TCG ATC TTG CGC CC-3′ was aimed against a distinctive series of rat PKCε. The matching GenBank (Country wide Institute of Wellness Bethesda MD) accession amount and ODN placement inside the cDNA series are XM345631 and 226-245 respectively. We've previously proven that vertebral intrathecal administration of antisense ODN with this series decreases PKCe proteins in dorsal main ganglia (Parada et al. 2003 The series from the mismatch ODN 5 AGC GCG ATC TTT CGC CC-3′ corresponds towards the PKCε antisense series with 2 bases mismatched (in daring typeface). Control animals received injections of mismatch ODN. Prior to use lyophilized ODN was.
Metazoans react to various forms of environmental stress by inducing the
Metazoans react to various forms of environmental stress by inducing the phosphorylation of the α subunit of the SF1126 translation initiation factor eIF2 at serine 51 (eIF2αP) a modification that leads to a global inhibition of mRNA translation. in mouse mammary gland tumors as well as in cells exposed to ER stress SF1126 or oxidative stress leading to the induction of cell survival or death respectively. In unstressed cells the PERK-eIF2αP pathway guards survival and facilitates adaptation to the deleterious effects of PI3K or Akt inactivation. As such inactivation of the PERK-eIF2αP arm increases the susceptibility of tumor cells to death by pharmacological inhibitors of PI3K or Akt. Thus in addition to mTOR the PERK-eIF2αP pathway provides a link between Akt signaling and translational control with implications in tumor formation and treatment. embryo Kc167 cells after treatment with LY294002 (fig. S2A). The efficacy of LY294002 and GDC-0941 treatments in all cells was documented by the reduction of Akt phosphorylation at S473 as well as inhibition of GSK3β phosphorylation at S9 (Figs. 1A-B fig. S1A S1B). To identify the eIF2α kinase implicated in this process we employed Kc167 cells to knockdown either dPERK or dGCN2 the two eIF2α kinases expressed in cells by siRNA. We noticed that siRNA-targeting of either dPERK (fig. S2B) or dGCN2 (fig. S2C) prevented the induction of deIF2αP by LY294002. Owing to the unavailability of antibodies for dPERK or dGCN2 we verified siRNA-mediated silencing by the lack of an induction of deIF2αP in Kc167 cells after treatment with inducers of each kinase such as thapsigargin (TG) (fig. S2B) and ultraviolet-C (UV-C) light (fig. S2C) which activate PERK (14) and GCN2 (15) respectively. These data implicated both dPERK and dGCN2 in eIF2αP in response to PI3K Rabbit polyclonal to PDCL3. inhibition. To substantiate these observations in mammalian cells we examined eIF2αP in MEFs lacking PERK and GCN2 (double knockout; DKO). We observed that unlike the wild-type (WT) MEFs induction of eIF2αP was not possible in DKO MEFs after treatment with LY294002 (Fig. 1C). Additional experiments with MEFs lacking either PKR (16) or HRI (17) indicated that neither SF1126 kinase is usually involved in eIF2αP by PI3K inhibition (fig. S3). That is additional backed by our latest function demonstrating that PKR mediates eIF2αP downstream of PTEN separately of PI3K signaling inhibition (10). Up coming we viewed the phosphorylation of Benefit at threonine (T) 980 an autophosphorylation site in the activation loop from the kinase that's needed for eIF2αP(14). We discovered that LY294002 treatment of WT MEFs resulted in a considerable induction of Benefit phosphorylation at T980 that was followed by a rise in eIF2αP (Fig. 1D). Benefit activation had not been due to an induction of ER stress because LY294002 treatment did not impact the splicing of X-box binding protein 1 (XBP-1) mRNA (fig. S4) which is as a reliable marker of UPR (18). Physique 1 Induction of eIF2αP by PI3K inhibition requires PERK and GCN2 Inactivation of Akt prospects to the induction of eIF2αP To determine the mechanism of PERK activation we employed Kc167 cells to knock-down dAkt by siRNA. We found that dAkt downregulation increased the basal levels of deIF2αP which however were not further increased after LY294002 treatment (fig. S5A). When WT MEFs and MEFs lacking Akt 1 and 2 (Akt DKO) (19) were used we observed that Akt1 2 increased the basal levels of eIF2αP compared to WT MEFs (Fig. 2A lanes 1 4 which was further increased after removal of the SF1126 remainder Akt3 by siRNA (lane 7). We also observed that treatment with LY294002 induced eIF2αP at a higher level in WT MEFs than in Akt1 2 DKO MEFs treated with either scramble siRNA (control) or siRNA for Akt3 (Fig. 2A). This data indicated that Akt downregulation increases eIF2αP which cannot be further increased by PI3K inhibition in Akt-deficient cells to the same extent as in Akt-proficient cells. To further support this data we employed pharmacological inhibitors of Akt such as inhibitor VIII and XI both of which target the pleckstrin homology (PH) domain name of Akt (20) or inhibitor IX which directly inhibits Akt activity(20). We observed that all inhibitors caused a substantial induction of eIF2αP in both mouse fibroblasts (Figs. 2B-D) and human tumor cells (figs. S5B-D). The efficiency of the treatments was verified by the impaired phosphorylation of Akt at S473 and ribosomal S6 protein at S235/236 (Figs. 2B-D; figs. S5B-D). Collectively these data supported the notion that Akt has a unfavorable effect on eIF2αP. Physique 2 Akt inactivation causes the induction of eIF2αP Akt inactivates PERK by phosphorylation at threonine 799 Mouse PERK contains seven serine.
Successful retrieval of a meeting includes a short phase where the
Successful retrieval of a meeting includes a short phase where the information is certainly accessed and a following phase where a person expands about event details. a graphic through the scholarly research phase. Retrieval was split into an initial memory space search and a following five-second elaboration stage. The current research identified neural variations between your search and elaboration stages with search becoming connected with wide-spread bilateral activations over the whole cortex and elaboration mainly being connected with improved activity in the medial prefrontal cortex. The emotionality from the retrieval focus on was more important during search in accordance with elaboration. Nevertheless valence affected when the result of feelings was biggest with search interesting many more areas for positive occasions than negative types but elaboration interesting the dorsomedial prefrontal cortex even more for negative occasions than positive occasions. 1 Introduction Effective retrieval of a SL-327 meeting includes two specific stages: a short stage in which info is seen and a following stage in which extra event information are retrieved. Typically functional neuroimaging research examining episodic memory retrieval either have not distinguished these two aspects of retrieval or have focused on regions recruited during the initial search process identifying a largely bilateral memory network that includes prefrontal (PFC) medial-temporal (MTL) medial-parieto-occipital lateral parietal anterior SL-327 cingulate occipital and cerebellar regions (see Cabeza and Nyberg 2000 and Spaniol et al. 2009 for reviews). Although the processes supporting elaboration of material presented in an earlier laboratory session have rarely been examined a number of recent studies have examined the neural correlates of search and elaboration phases during autobiographical retrieval (e.g. SL-327 Addis et al. 2007 Daselaar et al. 2008 Holland et al. 2011 finding a number of regions that are differentially recruited during these two phases. Compared to the elaboration phase autobiographical memory search has been associated with increased activity in the hippocampus (Daselaar et al. 2008 right dorsolateral and medial SL-327 PFC regions (Daselaar et al. 2008 and bilateral occipital gyrus (Addis et al. 2007 Elaboration has been associated with PRKM3 greater SL-327 activity in the left PFC ( Addis et al. 2007 Daselaar et al. 2008 right ventral PFC (Addis et al. 2007 left precuneus (Addis et al. 2007 Daselaar et al. 2008 and bilateral visual cortex (Daselaar et al. 2008 Despite some remaining ambiguity in the processes that distinguish search from elaboration the extant data suggest a dissociation between these phases within autobiographical memory. It really is currently unclear if the elaboration and search stages through the retrieval of laboratory-learned details may also dissociate. Previous research provides demonstrated significant distinctions in the neural and cognitive procedures supporting the seek out autobiographical in comparison to various other episodic event details. The original search stage of the voluntary autobiographical storage is a complicated iterative procedure that depends on storage search and managed retrieval procedures involving still left lateral PFC user-friendly monitoring procedures backed by ventromedial PFC and self-referential procedures backed by medial PFC (discover Cabeza & St. Jacques 2007 for review). Episodic seek out laboratory-learned details by contrast will involve a far more deliberate monitoring backed by dorsolateral PFC (Gilboa 2004 Discover McDermott Szpunar & Christ 2009 to get a meta-analysis) and it is less inclined to recruit the medial PFC for either user-friendly monitoring or self-referential digesting (Cabeza et al. 2004 Because of the significant distinctions between episodic and autobiographical storage search chances are the fact that distinctions between search and elaboration which exist for autobiographical storage may SL-327 not expand to other styles of episodic retrieval. As the majority of analysis evaluating the neuropsychology and cognitive neuroscience of storage has assessed storage for stimuli shown in the lab it is advisable to understand enough time span of the processes that support memory for these stimuli. It is also likely that characteristics of the remembered stimuli such as emotional valence may differentially influence the search and elaboration phases of episodic memory retrieval. Identifying the specific timing of emotion's.
Complicated community focused healthcare intervention and prevention partnerships fail or just
Complicated community focused healthcare intervention and prevention partnerships fail or just partially succeed at alarming prices. a ��near real-time�� feedback program (medical diagnosis and involvement) predicated on organizational theory social network theory and logic model metrics directed at partnership dynamics combined with logic model metrics. Keywords: Partnership failure Partnership success Logic model style Organizational theory Relationship best practices Wellness disparities evaluation Cancers plan evaluation Community involved evaluation Organizational lifestyle evaluation Social networking types of partnerships Baicalin 1 Launch Partnerships or organised collaborations across institutions are increasingly marketed as a technique to attain both synergy and performance. Organic research and educational partnerships have tremendous prospect of innovation failure and productivity at high cost. The organizational analysis books demonstrates that voluntary partnerships fail between 70 and 90 percent Baicalin of that time period particularly when the partnerships consist of some mix of business sector academics neighborhoods and federal government. The traditional deficit model on relationship failure is normally well noted (Arino & de la Torre 1998 Duysters Kok & Vaandrager 1999 Gulati Khanna & Nohria 1994 Meschi 1997 Nahavandi & Malekzadeh 1988 and you can find increasingly sophisticated guidelines models open to help overcome the deficits (Briody & Trotter 2008 Leonard 2011 Mendel Damberg Sorbero Varda & Farley 2009 Walsh 2006 Pursuing these cautions and versions we are determining our relationship (the Relationship for Local American Cancer Avoidance – NACP) being a voluntary association of people embedded within a formal cross-institutional collaborative plan (U-54). NACP is targeted with an over-arching group of goals goals and concepts for accomplishing analysis on cancer wellness disparities in Local American neighborhoods. The partnership provides both formal and casual elements (goals goals processes behaviors) which are accomplished through both formal and informal activities and associations. In spite of the grim statistics on partnership failure the Baicalin federal government is constantly funding Baicalin large multisite multi-institutional projects like MGC126582 NACP that are designed to produce breakthrough ��translation technology�� for health and medical care improvements. These advancement partnerships are progressively complicated and often made up of ��silos�� seeking to become integrated systems. Examples of ��silos�� abound in academic and study businesses and exist in areas Baicalin along with other partnerships also. A silo can be an organizational framework that is centered on the effective completion of a comparatively narrow but essential area of the organizational objective so when a consequence is normally resistant to intrusions (or extrusions) that problem the prevailing paradigm. Also within one educational or analysis institutions educational departments frequently have practically impermeable limitations that decrease the opportunity for combination- multi- and inter-disciplinary imagination cooperation and efficiency. This matter Baicalin becomes a lot more problematic when multiple institutions making use of their own silos are participating each. The overall objective of NACP would be to connect research workers across disciplines also to integrate the occasionally disparate missions of outreach to neighborhoods and reviews from neighborhoods (especially ones which are considerably culturally not the same as the colleges and research workers) while helping the differing missions of schooling and analysis. Such connection and integration must intentionally span natural and artificial boundaries (i.e. the boundary or ��siloing�� mechanisms natural to people who effectively live in a different social milieus). One result of the need to reduce ��siloing�� is to create an evaluation model that shows the integration of organizational devices across boundaries as well as showing the impact on diversity of viewpoint on productivity of the devices within those boundaries. A recent review (Uzzi Mukherjee Stringer & Jones 2013 found that medical publications bringing or citing novel perspectives into an normally ��standard�� approach were likely to have higher than normal citation rates (interpreted as having the highest effect overall). Such papers were more often produced by teams of collaborating authors than by solitary authors. Thus the success of teams in melding variations of perspective could be vital to reaching the.
There is some evidence that college student drinkers may continue drinking
There is some evidence that college student drinkers may continue drinking in the face of adverse consequences. of odds ratio = 1.17 to 1 1.45 across semesters < .01). Such potent effects may predict subsequent drinking for a number of possible reasons that may be examined and addressed as they would pertain to specific protective behavioral strategy-related and cognitive interventions. Furthermore effects were accumulated over multiple semesters by notable proportions of students. For example 13.8% of students reported blacking out 5 time-points or more-describing a full half or more of their college careers. Experimental studies which aim to change students' perceptions of norms associated with these effects may aid in developing interventions to reduce the burden of harm to students. In the broader context and given the prevalence of students' accumulation of effects future study might aim to determine how and in what ways these findings KN-92 phosphate describe either pathological or normative processes. of such effects is likely quite prevalent given the findings on students' going through of repeated outcomes in their 1st year of university only (Mallett Marzell et al. 2011 It ought to be mentioned that in medical contexts the build up of outcomes is considered a significant system of behavior modification (Matzger Kaskutas & Weisner 2005 which may also keep for general scholar populations (Go through Wardell & Bachrach 2013 whereby KN-92 phosphate outcome accumulation takes on a normative part in people' eventually "maturing out" of weighty consuming (Arnett 2006 Schulenberg & Maggs 2002 Schulenberg O'Malley Bachman Wadsworth & Johnston 1996 Both for scholars who strategy the build up of outcomes as a report of pathology and for individuals who treat it as a report of normative advancement it's important to record the full degree of such build up during an entire university career. Therefore we aimed to reproduce and additional explore results that college students KN-92 phosphate drink despite outcomes extending observation to add a 4-season course of the faculty experience. That's commensurate with the idea how the trend might be noticed generally through the entire entirety of university we hypothesized that people would observe some outcomes that would regularly predict subsequent taking in throughout university. Identification of the strongest or time-invariant outcomes would (a) offer further support because of this trend of college students' consuming despite outcomes being one which may be descriptive of university generally (and for that reason placing the stage for long term explanatory hypotheses CSMF that focus on general social cultural learning-related and norms procedures because they KN-92 phosphate pertain to the entire university encounter; Astin 1993 and (b) inform book intervention efforts targeted at college students' perceptions decisions or behavioral strategies around these specific of outcomes (discover Martens Martin Littlefield Murphy & Cimini 2011 Piasecki Sher Slutske & Jackson 2005 Not merely did we be prepared to observe particular of outcomes that could generally predict following drinking throughout university but we also hypothesized that folks would these outcomes over multiple semesters over university in a way that a significant proportion of several college students' university experience will be referred to by repeated or gathered outcomes of particular types. Once again this book second little bit of information can be utilized by scholars to either formulate even more particular hypotheses about systems of modification in clinical college student populations or even to formulate even more particular hypotheses about normative systems of development. Technique Participants Following authorization from the college or university Institutional Review Panel an example of 3 720 first-time undergraduate college students (88% from the getting into course) was asked to full a paper-and-pencil study the summer ahead of university admittance in 2002. More than another 4 years individuals had been asked to full an paid survey at each successive semester. The baseline test was 53.6% female 90.3% White/non-Hispanic and averaged 17.96 (= .37) years. The test size dropped to = 2 250 by the ultimate time-point; though remember that 90% of college students participated in several evaluation waves and 82% participated in three or even more waves. Retention biases.


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Need spring? Photos of Boston's Flower & Garden Show
The 146th Boston Flower and Garden Show
The 146th Boston Flower and Garden Show is taking place at the Seaport World Trade Center through March 16, with the theme "Romance in the Garden."
"People think it's very bold for us to put a bed in the garden," said Michael Papandrea, an exhibitor for a garden landscaping company. "You know the theme is about romance."PHOTO: Kiva Kuan Liu
A mini garden at the show.PHOTO: Kiva Kuan Liu
A landscape example on display.PHOTO: Kiva Kuan Liu
From landscaping examples to gardening tools, from real plants to garden decoration, there are 265 booths at the show.PHOTO: Kiva Kuan Liu
The small artificial plants at the show.PHOTO: Kiva Kuan Liu
A garden decoration.PHOTO: Kiva Kuan Liu
Real cakes with flower decorations.PHOTO: Kiva Kuan Liu
Stan Jackson (left) and Mary Lisa came to the Flower and Garden Show five years ago when they just met. The now-married couple visits the show every year to get a sense of the decoration of their home. "It's like the best date for us," said Lisa. "I enter a different world."
PHOTO: Kiva Kuan Liu
Visitors learn to arrange flowers together. There are other lectures like "Sustainable Lawn Care" and "Master Food Preserver" available.PHOTO: Kiva Kuan Liu
Pussy Willow is one of the most popular plants in the show.PHOTO: Kiva Kuan Liu
"Based on the winter we have, this is a great celebration of the warm weather to come," said Alexandria Pudney, the Show Producer. "People have forgot what spring looks like, so here we are."PHOTO: Kiva Kuan Liu
A vendor is selling bamboo pillows. The show producers rent booths to vendors who sell different products including jewelry and shoes, to keep the diversity in the show.
A boy visits a garden at the Flower and Garden show.PHOTO: Kiva Kuan Liu
Eleanor Pupa (right), 92, comes to this show almost every year. She dressed up fo Saint Patrick's Day because she remembers the show is always on the weekend of Saint Patrick's DayPHOTO: Kiva Kuan Liu


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Zesty Herbed Mushroom-tomato Sauce
32 ounces canned tomatoes -- cut up
2 cups fresh mushrooms
6 ounces tomato paste
1/2 cup onion -- chopped
2 cloves garlic -- minced
2 tablespoons parmesan cheese -- grated
2 teaspoons dried oregano -- crushed
2 teaspoons brown sugar
1 1/2 teaspoons dried basil -- crushed
1/2 teaspoon salt
1/2 teaspoon fennel seed
1/4 teaspoon crushed red pepper
1 bay leaf
12 ounces spaghetti -- cooked and drained
6 tablespoons parmesan cheese -- grated
Place all ingredients in slow cooker and combine. Cover and cook on low for 8 to 10 hours or on high for 4 to 5 hours. Disgard bay leaf. Serve over the cooked pasta. Sprinkle with cheese.
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Biblically Committed, Historically Reformed
Welcome to Reformation Presbyterian Church! As a new church in Virginia Beach, VA, we want to be true to what the Bible teaches and what Christians have historically believed.
We are a mission work of the Orthodox Presbyterian Church (OPC). We believe that the living and true God, our triune Creator, Father, Son, and Holy Spirit, has called on people everywhere to worship Him in spirit and in truth. Come hear the good news from the Bible that reminds us of the Lord Jesus Christ, the substitute for sinners, and the new life that those who believe in Him have in His name.
Lord's Day Services
11:00 am Morning Worship
6:00 pm Evening Worship
Both services on the Lord's Day now meet at the Indian Lakes Community Center. Get directions
Sermon Series
The Book of Mark (AM)
Psalm 23 (PM)


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REUTERS/Jacky NaegelenThe INSIDER Summary:
• Sometimes when you run into Sephora just to buy a lipstick you end up leaving with much more.
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Oil on wood panel
10 x 8
Ten Stone Tea was a bit of an adventure for me. I had been feeling artistically weighed down with a seemingly endless parade of freelance jobs not to mention my full time, nine to five job. After I finally got a little bit of time for myself I decided that to keep myself fresh and to recharge my batteries I would do a painting just for me. This is the result.
To tell you the God's honest truth I wasn't really sure of what to paint when I started and frankly most of the elements in this painting are stuff I put in on the fly. In the end however everything came together quite nicely and now I have a much stronger appreciation of my subconscious.
I would also like to note I was so enamored with the method and the style of this painting I decided this would only be the very first in a long series of paintings that I will at some future date collect into a book and publish.
This is another of my latest, "Painting by the seat of my pants" (Or panting by the seat of my paints…) I'm not sure if it really fits the mood of the previous piece or if it'll ultimately end up in the "book" but for right now I quite enjoy the feeling of this image. Admittedly the background is a little plain but as fellow artist Jim Goodliff pointed out, it kind of suits the piece.
Furthermore I would like to point out that the pattern on the wall is the brainchild of my lovely wife Halima. I had originally planned on putting a row of Trilobite fossils embedded into the wall but after describing the piece to her she suggested using the wishbones instead. Just goes to show that first, one need not be an artist to be creative and second, I'm anything but infallible.
Digital- Painter 8
6 x 8
I've always enjoyed self portraits. Not necessarily doing them but looking at what other artists do. I think a self portrait is really an unabashed view of how an artist sees himself, or more to the point how they think the world percieves them. I was recently asked to do an interview for a webzine and as part of the bit I was asked to include a headshot or a self portrait. Because every single photograph ever taken of me makes me look A) retarded B) ugly or C) both, I opted for the self portrait. This is what came out.
I guess I think the world sees me as some sort of floaty-head macabre, ghost-pirate that's into carving numbers on their forehead.
Maybe I need to rethink things


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Our physical therapists have extensive experience and education on the evaluation and treatment of sports related injuries. Whether you are a weekend warrior or an elite athlete, we have the skills to get you back to sports and competition.
The co-owner of Denver Back Pain Specialists, Dr. Amy Valenta PT, DPT, OCS, has travelled with the USA Rugby team for the last 10 years both domestically and internationally and understands what it takes to get an athlete back on the field at a competitive level as quickly as possible. Our therapists specialize in treatment techniques not only to decrease pain associated with an acute injury or surgery but also to decrease the aches and pains associated with daily life. They provide one-to-one treatment, which includes manual therapy techniques, therapeutic exercise, neurology re-education, modalities, and specific exercise instruction in order to establish a program specific to your goals and individual needs.
Our physical therapists are specialized in manual therapy techniques and the treatment of orthopedic injuries.
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Sector: Law | Size: 5,500 sq ft | Location: London
A bright and modern office to reflect Michelmores high profile.
Interaction were delighted of the completion of works at Michelmores new Chancery Lane offices. A modern & inspirational office design has been implemented to reflect the companies standing as a leading law firm in the UK.
The reception area is light, spacious & welcoming ensuring a memorable great first impression for all visitors. Brand image has been used throughout the offices to create an engaging workplace. Informal meeting areas have been used to create a variety of areas for employees to work together.
A light and inspiring office space has been created that enables collaboration but also privacy when needed. Meeting rooms are flexible, spacious & comfortable ensuring the space can be used for a variety of functions.
The kitchen is a place where employees can meet informally or relax in break periods. Strong confident colours have been used to create a great flexible space.
Michelmores are delighted with the outcome:
"We love it. Really love it!"


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Title: Here I Come
Personal Fanfic Community: dfasgbaf (Fics post here first)
Personal Fanfic Group: DFAS Giles Bloody Awful Fanfic (Forget to post here a lot)
Fandom: BONES
Characters: Special Agent Seeley Booth and Dr. Gordon Wyatt
Rating: FIC-PG
Genre: ...Character Development?
Word Count: 522 *weak smile*
Spoilers: Up to and including the episode "Bodies in the Book." Read no further in these headers if you do not want to be spoiled for that show.
Summary: After his reaction to seeing Sully and Temperance kiss, despite his hand in it, Booth finally breaks down and goes to Dr. Wyatt to discuss a topic that has nothing to do with work--well, not professionally, anyway.
Disclaimer: BONES is from Josephson Entertainment and Far Field Productions in association with 20th Century Fox Television. Barry Josephson and Hart Hanson are executive producers, and the characters Seeley Booth and Dr. Gordon Wyatt belong to them. No one receives profit from this. I write it only for guilty pleasure and to further the fan base of a great show.
Feedback: Constructive criticism, both negative and positive, will be accepted with grace and gratitude. Destructive criticism (insults without examples from my work and suggestions for improvement to fortify the insults) will be deleted and will earn a block from journal, community, inbox, and/or group. Praise is always accepted, of course!
Author's note: Thank you, catvampcrazines, for adding Bones to the list of fandoms in btvsats_love! This fic was written in response to the prompts "scary" or "creepy."
Distribution: Archive sites, etc., of which I am a member. Anyone else, please ask here. If you're willing to share the archive info with me, I will probably say yes. Thanks for your interest!
"So," Dr. Wyatt said, settling into the patio chair across from Booth with his freshly brewed cup of tea, "tell me why you've come to visit me, Agent."
Booth shifted in his seat, the wood legs of the chair creaking under his movements. "It's...my partner."
Doc blinked once, twice, wide-eyed. "Oh, yes? What about the good doctor?"
Booth shifted again. "Well, you know...I told you...they put her...with someone else...while I was out because I was busy with you."
Doc leaned back, cradling his chin with his hand and tapping it with his forefinger. "Ah, yes. I do recall you mentioning that. Go on."
"Well, it turns out...they kinda...liked each other-- " he winced-- "so, as soon as I get back, he comes to me asking for advice--"
"Which you naturally gave--"
"What?" Booth sat straight up, eyes wide. "Of course, I didn't! Why would I give someone advice on how to get my partner into bed?"
"Oh, come, now, Agent," Doc said with a conspiratorial grin, "you're Agent Sullivan's friend, so you know he's not a predatory monster...and your partner's quite the head turner. Why not--how do you Americans say it?--hook him up?"
More fidgeting. "Because!" Booth said with passionately angry heat in his eyes.
"Because you love her?"
"Yes!" Caught by his own reflexive answer, Booth jerked, then quickly followed with, "No!" On Doc's knowing smile, he continued, "Okay, yes, I love her. She's my partner. You know partners share a special bond. Look at Sully. He's mostly been a desk jockey since he lost--"
Doc clucked his tongue. "You know very well that's not what I meant."
A puff of air fumed out of Booth's nose as he gazed at the fists clenching each other on his knee. "I know."
"Well? What are you going to do about it?"
"What can I do about it?" Booth looked at him with wide, sad eyes. "I waited too long because I was afraid she'd never want a relationship with someone at work. Now, every time I turn around, she and Sully are acting like a couple of kids by their lockers in high school, making eyes, sneaking kisses....It's creepy."
Doc tucked his chin into his chest. "Creepy?"
"Yeah. To see her letting a guy that close. Before now, I was the closest, and I never...." He sighed.
Doc nodded. "But you were beginning to hope you might."
Booth looked down. "Yeah. I just--I wasn't ready to admit it."
"The nudges we need can, at times, come from unfortunate places, Agent Booth." Patting his legs, Doc pushed to a standing position, taking hold of his teacup. "I suggest you consider all of your options and their possible consequences very carefully over the next few days, Mr. G-man, as well as the old adage, 'You never know until you try.'" With that, he went inside his house and closed the door behind him.
Booth just sat staring at the table. "You never know until you--" He smiled brightly. "Oh...what the hell?" Standing up, he straightened his tie and lapels. "Look out, Sul. Here comes the competition."


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I am a lecturer at the AUT Law School, specializing in constitutional law. I have completed my studies for a J.S.D. at the NYU School of Law, and should get the degree in a not-too-distant future. I also have an LL.M. in Legal Theory from NYU; clerked for Justice Tremblay-Lamer at the Federal Court of Canada; and have a B.C.L./LL.B. (Hons.) from McGill. A list of my publications is below.
The purpose of this blog is to offer some comments on Canadian constitutional law, legal and political philosophy, and other (law- and political philosophy-related) topics that interest me.
My introductory post, explaining why I decided to do it, is here.
You can contact me at leonid.sirota at aut.ac.nz.
My publications:
- (With Maxime St-Hilaire) "Canadian Voter Identification Requirements in a Comparative Perspective", in Gregory Tardi (ed.), The Informed Citizens' Guide to Elections: Electioneering Based on the Rule of Law 517 (2015)
- "'Third Parties' and Democracy 2.0", 60(2) McGill Law Journal 253 (2015)
- "True Allegiance: The Citizenship Oath and the Charter", 33(2) National Journal of Constitutional Law 137 (2014)
- (with Fabien Gélinas) "Constitutional Conventions and Senate Reform", 5 Revue Québécoise de Droit Constitutionnel 107 (2013)
- "Storm and Havoc: Religious Exemptions and the Rule of Law", 47(2) Revue Juridique Thémis de l'Université de Montréal 247 (2013)
- "Towards a Jurisprudence of Constitutional Conventions", 11(1) Oxford University Commonwealth Law Journal 29 (2011)
In a professional journal
- "Rants and Freedoms: Can Universities Control Students' Online Speech?", 13(2) Internet and E-Commerce Law in Canada 13 (2012)
In the general media:
- "There Will Be No Harper Revolution", Policy Options, October 2015
- "The expat vote: What we're getting wrong, and why it still matters", OpenCanada.org, July 29, 2015
- "The Supreme Court versus Economics", The National Post, February 19, 2015 at A19
- "Le mirage de la neutralité de l'État", La Presse.ca, August 16, 2012
- "Élections: les étudiants bâillonés?", La Presse.ca, April 14, 2012
I also contribute, regularly or occasionally, to other blogs: the CBA National Magazine's Blog; that of Policy Options; and I-CONnect.
In addition, almost all of my posts commenting on recent judicial decisions are cross-posted (sometimes with some modifications) on CanLII Connects.


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João Frederico Ludovice
From Wikipedia, the free encyclopedia
(Redirected from Ludovice)
Johann Friedrich Ludwig (1673–1752), known in Portugal as João Frederico Ludovice, was a German born architect and goldsmith.
Ludovice was born in Hohenhart. In 1698 he went to Italy, where he married and converted to Catholicism, changing his name to Ludovici. He worked in the altars of the Church of Saint Ignatius of Jesus and received the influences of masters as Carlo Fontana and Andrea Pozzo. The Jesuits admired his work and talent, inviting him to work in Portugal. There he became famous by the project for the Mafra National Palace (1717–1731) for the Portuguese king João V, among many other monuments, including churches and the tower of the University of Coimbra.
He later adopted Portuguese citizenship. He died in 1752.
|This article about a Portuguese architect is a stub. You can help Wikipedia by expanding it.
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1925 $1 Brilliant Uncirculated NGC. NGC Census: (20/43796). PCGS Population (59/38221). Mintage: 10,198,000. Numismedia Wsl...Click the image to load the highest resolution version.
1925 $1 Brilliant Uncirculated NGC. NGC Census: (20/43796). PCGS Population (59/38221). Mintage: 10,198,000. Numismedia Wsl. Price for problem free NGC/PCGS coin in MS60: $34.(Registry values: N69) (NGC ID# 257L, PCGS# 7365)
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This is a summary of all the Logbooks (Travelogs) related to ホースシューベント that we have available for the United States area. Please go ahead and click on them if you want to read their contents. Does reading about ホースシューベント in United States make you want to go there too? In that case let's start planning your next trip!
2015-02-14 - 2015-02-15


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A life forged in tragedy, but defined by triumph.
One of the most inspirational books you will ever read, Radical Survivor is ultimately an uplifting tribute to the human capacity for healing and resilience.
With the added richness of letters Nancy received before and after the crash, as well as her sons' journal entries, Radical Survivor traverses the full spectrum of human emotions, offering a gift of hope for anyone faced with healing after loss of any kind.
©2012 Nancy Saltzman (P)2014 Nancy Saltzman
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BP-TNK, Russia Oil and Gas
Lukoil considers selling a third of its petrol stations in Russia.Edited by Rhod Mackenzie / 2017-03-28 11:09:58
LUKOIL is considering the possibility of selling up to 30% of its petrol stations in Russia due to a drop in the profitability of the business.
Rosneft to transfer all TNK petrol stations to its brand.Edited by Rhod Mackenzie / 2017-03-14 10:27:57
Rebranding can cost from 1.5 billion to 4 billion roubles.
BP not to participate in the privatization of "Rosneft".Edited by Rhod Mackenzie / 2016-06-16 10:00:47
Company is satisfied with its current status of a major minority shareholder of the state-owned company.
"We are satisfied with interests in "Rosneft" I think we'll keep it at the current level", - CEO BP Robert Dudley stated, commenting on the possibility of buying additional package. The company would neither increase nor reduce the share of "Rosneft", the representative of BP added. Available package (19.75%) corresponded to the strategic interests of BP to work in Russia, he stated, refusing to comment further.
The RF Ministry Finance expects to sell 19.5% of Rosneft in H2 2016.Edited by Rhod Mackenzie / 2016-04-19 01:53:12
In H2 2016 Russian authorities were going to have time to sell 19.5% of Rosneft, the largest state-owned oil company of Russia, and expected to receive about 650 billion roubles, Prime stated with reference to the statement of the RF Minister of Finance Anton Siluanov in an interview with the CNBC.
At present the capitalization of the largest oil companies of the country was 3.27 trillion roubles, so Anton Siluanov announced market price for the government stake.
Rosneft may receive 60 billion rubles from budget next monthEdited by Rhod Mackenzie / 2016-02-16 09:10:19
Rosneft has been very close to the receiving of 60 billion rubles from the budget. The company is seeking the return of overpaid customs duties on oil on its asset Verkhnechonskneftegaz through court and the Energy Ministry hasn't managed to convince the Supreme Court to suspend the decisions of previous courts that took the side of Rosneft. Now the company, if it applies for the receipt of money, will be able to get them for a month. But the Ministry of Energy still has a chance to convince the Supreme Court to review the case.
The Supreme Court refused the Ministry of Energy to suspend the decisions of the appellate and cassation courts in the dispute with Rosneft of overpaid customs duties on oil from the Verkhnechonskoye field in the Irkutsk region. The Ministry of Energy filed the relevant complaint on February 10. Now the Ministry is obliged to implement the decision of the courts and to issue Rosneft a certificate of confirmation of "special physical and chemical characteristics" of oil from this field. In turn, this will give Rosneft the right to claim a refund of the customs duties in the amount of 60 billion rubles.
"Rosneft" stands for reference.Edited by Rhod Mackenzie / 2015-10-12 09:11:07
The RF Ministry of Energy is in no hurry to recognize VChNG extraction.
At the request of the RF Ministry of Energy cassation court froze the execution of the decision the Court of Appeal, which supported the requirement of "Rosneft" on the recognition of backdating its eligibility on oil privileges at Verhnechonsk field. Now, before the completion of the lawsuit the company can not get the proper reference from the RF Ministry of Energy and begin the process of recovery of about 60 billion roubles of the overpaid export duty. Lawyers believe that the decision of the appeal court can tell us about the next turn of the case not in favor of "Rosneft".
Rosneft's deal on VNHK may require coordination – FAS.Edited by Rhod Mackenzie / 2015-09-09 09:28:24
The deal of Rosneft, the largest state-owned oil company of Russia, and China National Chemical Corporation ChemChina for the purchase of shares of "Eastern Petrochemical Company" (VNHK) may require prior approval of the law on foreign investment, the website of the agency informs referring to the statement of Armen Han, the head of the control of foreign investments FAS RF.
Last week Rosneft and ChemChina signed a memorandum of understanding on cooperation on the VNHK project. The memorandum concerns the proposed acquisition of a controlling stake in VNHK by ChemChina and identifies key stages of investment.
"Rosneft" to require debt restructuring.Edited by Rhod Mackenzie / 2015-09-02 09:11:59
The company disclosed the debt repayment schedule.
By the end of the year "Rosneft", the total debt of which amounts to $ 54.3 billion, expects to return nearly $ 11 billion to creditors, and in 2016 - even more than $ 15 billion, instead of the previously planned $ 13.3 billion. Despite this, the company plans to increase capital investments by 30 % compared to 2014, although it admits that may reduce investment. Experts believe that the implementation of plans for the next year will require the oil company to maintain free cash flow and restructure around half of the amount of duty payable.
Leontiev: "Rosneft" becomes a victim of bad press campaign.Edited by Rhod Mackenzie / 2015-08-26 10:43:05
An obvious bad press company was being held against "Rosneft" in a dispute over a license to the East Taimyr section, the vice-president Mikhail Leontyev stated. "Custom campaign that is now in the press is quite obvious: it begins, as always, with internet garbage dumps. We will not spend money on the black PR and the purchase of some anonymous writers. We can openly explain everything and openly present our arguments, and most importantly - dispel such nonsense, misinformation and those insinuations that afford our opponents on this particular dispute addressing "Rosneft", which, in our view undermines the company's image and harms our business reputation and even in general terms are unacceptable within the country", - Leontyev stated.
Rosneft proposes Siberian deposits to the United Arab EmiratesEdited by Rhod Mackenzie / 2015-08-25 09:18:46
Rosneft tries to attract UAE's Mubadala Petroleum in projects of the Srednebotuobinskoye and Verkhnechonskoye fields development in Eastern Siberia. For the first of them Rosneft has already found a partner - British BP, but the share in Verkhnechonskneftegaz as an asset for sale is mentioned for the first time. These are key new projects of Rosneft, and, according to analysts, given the fall in oil prices and high debt load, the company can't do without serious partners.
Rosneft is in talks with the United Arab Emirates' investment fund Mubadala Petroleum (a part of the Mubadala group) on participation in two projects in East Siberia, the Kremlin's press centre reported on the eve of Vladimir Putin's meeting with the Crown Prince of Abu Dhabi Mohammed Al-Nahyan. We are talking about the development of the Srednebotuobinskoye and Verkhnechonskoye fields. Rosneft declined to comment, but the company has reached an agreement with Mubadala Petroleum on strategic cooperation in projects in Eastern Siberia, East Asia and south-east Africa during the St. Petersburg Economic Forum in May 2014.


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Art of A Cappella Workshop
Interested in learning more about the worldwide phenomenon that is a cappella (as seen in Pitch Perfect and Pentatonix)? Want to improve your musicianship and performance skills? In this series of introductory workshops, you'll learn the basics of beatboxing, vocal arranging, healthy singing, and ensemble skills in the a cappella style. No audition required!
Workshops are open to the entire Hunter College community, including undergraduates, graduates, faculty, and staff. Come join us if you love to sing!
Dates: Wednesdays (May 3, 10, 17)
Time: 3:00 pm - 5:00 pm
Place: Room 406, North Building
For more information, please contact the director of the A Cappella workshops, Kari Francis, at firstname.lastname@example.com.


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This centrally located facility has large conference rooms, small meeting rooms, outdoor balcony areas, barbeque facilities, and fantastic views over Wellington Harbour.
Centrally located with a fantastic view over Wellington Harbour, the Wellington Conference Centre features three large conference rooms, video conferencing facilities, five smaller meeting rooms and three outdoor balcony areas complete with barbecue facilities. All rooms have natural lighting and have been architecturally designed with a contemporary feel. Accommodating up to 110 people, the centre is ideal for a variety of corporate events, from small meetings to all day workshops, seminars and after work cocktail functions.
The large conference rooms are fitted with a combination of interactive whiteboards, drop down high resolution data projectors and drop down projection screens, while the smaller meeting rooms feature interactive whiteboards and high resolution 42 inch commercial plasma screens. There is also have a dedicated, purpose-built video conference facility with twin 50 inch commercial plasma screens, enabling meetings to include multiple remote sites and audio participants.
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Staff Support
The Staffing Council serves as the state's recruitment advisory and coordinating group. The council works collaboratively with OFM State Human Resources, DES Workforce Support and Development, and agency HR offices to provide recommendations on strategic staffing efforts across state government.
The Staffing Council meets on the second Tuesday of each month from 9:00 a.m. - 11:00 a.m. at OFM State Human Resources


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Ano Hi Mita Hana no Namae wo Bokutachi wa Mada Shiranai. Movie
Latest Episode
Episode Movie
The TV series is retold through the different main characters' points of view with new additional material from their childhood, during the original series, and one year after the conclusion of the series.
- Japanese: 劇場版 あの日見た花の名前を僕達はまだ知らない。
- Type: Movie
- Episodes: 1
- Status: Completed
- Aired: Aug 31, 2013
- Producers: Aniplex, Aniplex of America
- Genres: Drama, Slice of Life, Supernatural
- Duration: 1 hr. 39 min.
- Score: 8.21
- Fansub: Crunchyroll / Funimation
Ano Hi Mita Hana no Namae wo Bokutachi wa Mada Shiranai. Movie Episodes List
- Episode Title Date Added Watch
- Episode Movie 7 months ago Start Watching!


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The Delta Mix-Up Cups come as a set of 10 graduated mixing cups, that are made of shatterproof polyethylene, and are clearly marked on the sides in both ounces and milliliters.
The set comes with a 30 ml size up to a 600 ml cup.


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Residents of Altus and surrounding Jackson County are celebrating Rock N Rumble, Cruisin' with Main Street for an 18th season.
The Altus Live group is set to kick off events 6 p.m. Friday on the downtown square with the band Bryce Dicus and the Mercenaries. Vendors are scheduled to be set up on the Jackson County Courthouse parking lot.
"We are excited to celebrate the 18th year of Roll N Rumble and we encourage everyone to visit the main street office at 100 North Main St. to purchase a T-shirt for $15, which helps to fund many other Main Street Altus events," Main Street Altus Director Amy Jo Cobb said.
Cobb said four Altus car dealers take turns sponsoring the official Rock N Rumble T-shirt and this year sponsor is Putnam Toyota.
There are other activities in conjunction with the Rock N Rumble, such as the Disabled American Veterans' Pancake Breakfast at the Towers from 7 a.m. to noon; the Motorcycle Poker Run, Show and Cruise sponsored by Altus/Lawton Motorsports; the Burn Out Contest sponsored by the Altus Firefighters Combat Challenge Team; the Hot Wheels Car Show sponsored by the How To Palace; the Stereo Sound off Competition sponsored by Smiley's Tires, Tunes and Tints; the Boy Scout Pinewood Derby sponsored by Century 21 Altus Prestige Realty and the free outdoor concert featuring Roy Lee Scott "The Flying Cowboy."
Tailgate parties will be positioned along the cruise route. The Saturday night cruise is from 7-9 p.m. and is open to everyone with a cruise pass.
The Main Street Altus office will be open all week from 9 a.m. to 5 p.m., except Friday when it closes at 4 p.m.
On Saturday, Main Street Altus will have a tent set up on the square, near downtown on Hudson Street at 7 a.m. This will be the last opportunity to register a vehicle or motorcycle, or buy a T-shirt. For more information call 580-482-2277.
Reach Mary O. Esparza at 582-482-1221, ext. 2077.