text:
Terms & Conditions
Entry opens from 7th December to midnight 18th December 2015. For the chance to win the prize that day entry must be made between 12:01am – 11:59pm. Winners chosen at random, each winner will be contacted by email within 5 days. Confirmation will need to be received from the winner within 7 days of email contact or a new winner will be picked. Each prize is stated as is for the specified day, however the prize is liable to change and cannot be exchanged for cash, gift vouchers, or any other item. OnlineGolf's decision is final. Prizes can only be sent to UK or EU addresses. Full address will be required for delivery. Please note if you do not wish to receive emails from OnlineGolf you need to select the option above. Good luck!


text:
- Hi there, welcome to Tribesmart. Log in to get started...
Mint Green 3d Guipure Floral Lace Front T-shirt
Guide price: £18
Last updated on 25th Jun 2014
Tags: clothing accessories womens
Mint Green 3d Guipure Floral Lace Front T-shirt
Whether layered with denim dunagrees or paired with a leather skater skirt, this raised lace panel t-shirt is a wardrobe essential.- Simple short sleeves- Textured finish- Classic crew neck
Login or Register to add this to your wish list or favourites.
Similar to Mint Green 3d Guipure Floral Lace Front T-shirt
Madam Rage Cream Polka Dot Contrast Collar Lace Skater Dress
Madam Rage. Whether worn to work, or for everyday casual wear, this lace skater dress is ideal.- Simple short sleeves- Contrast collar- Model is 58 and wears UK 10/EU 38/US 6
Stick Necklace In Gold
This stick necklace oozes art deco charm, crafted from gold-tone metals with a delicate pendent design.
Minkpink Blue Blooms Bikini Bottoms
These bikini bottoms from Minkpink have been crafted with eye-catching bold floral blooms for an offbeat addition to your holiday wardrobe.THINGS TO KNOW:- Composition: 80% Polyamide, 20% Elastane- Care: Hand wash
Plus Size Patchwork Lace Mini Dress
If you are searching for woman designed dresses within your budget browse: Jollychic. We are a leading online shopping site providing latest stylish woman party dresses, summer dresses, shoes, accessories and many more. Shop our collection of high quality designed clothes that you are sure to adore. Shop this Plus Size Patchwork Lace Mini Dress from Jollychic @ US$ 21.99 For more info visit http://www.jollychic.com/p/plus-size-patchwork-lace-mini-dress-g187203.html

subset: commoncrawl


text:
Congratulations are in order for Uma Thurman. The actress is pregnant, Life & Style reports.
The new addition will be the first child for Thurman and her boyfriend, Arpad Busson, though she has two children, Maya, 13, and Levon, 10, with her ex-husband Ethan Hawke.
"She's excited," the insider told Life & Style. "She's happy and just a few months along right now. She's due in late, late summer."
Though the baby news just came out this week, friends have reportedly speculated that she was expecting after she appeared all covered up while attending New York Fashion Week events earlier this month.
Thurman isn't the only celebrity who's expecting. Check out other pregnant stars below:
Get top stories and blog posts emailed to me each day. Newsletters may offer personalized content or advertisements.Learn more


text:
Sold out…
Free People White Endless Summer Womens Dare To Bare Maxi
See more Free People Dresses.
Get notified when it's back…
We check over 450 retailers daily. Be the first to know when it comes back in stock.
See more Free People Dresses.
We check over 450 retailers daily. Be the first to know when it comes back in stock.
Made from our sheer and gauzy Endless Summer fabric, this statement-making maxi features strappy centre cutouts and a high neckline. Sweeping silhouette and daring open back with braided ties at neck, featuring whimsically beaded ends. Throw on top of a bikini or layer over one of our seamless styles for an effortless look. Endless Summer. Gauzy, sheer silhouettes for effortless layering under the sun. Whether you live the beach lifestyle year-round or dream of making the great escape, explore the Endless Summer collection of: dreamy maxi dresses, tunics, rompers, and more. Measurements for Small: Bust: 33 in = 83 3/4 cm Waist: 36 in = 91 1/2 cm Length: 64 in = 162 1/2 cm. Color: white
Free People items by category | All Free People items | All Women's Dresses


text:
Invaluable cannot guarantee the accuracy of translations through Google Translate and disclaims any responsibility for inaccurate translations.
Palm Beach Modern & Antique Estate Clearance Auction
by MG Neely Auction
13 November 2016, 12:00 EST
Lantana, FL, USALive Auction
Lot 226: (8) Vintage Sterling Silver Money Clips(51 views)
|Starting bid:
|Your max bid:
The Invaluable Live! SecureBid™ feature ensures you never overpay. Without disclosing your limit to anyone, SecureBid™ places your bids automatically, up to one increment higher than the last in-room bid or preset reserve, until we have reached your maximum bid.Close
(Enter more than $40)
Estimate: $80 - $100
Description: (8) Vintage sterling silver money clips: (1) "PRK Sterling" engraved clip. (1) "Admark Sterling" clip (with empty monogram field). (1) Mexican clip with silver horseshoe inlaid in turquoise and a cut-out dollar symbol on reverse side. (1) Mexican clip with silver horseshoe inlaid in mother of pearl and a cut-out dollar symbol on reverse side. (2) Mexico silver clips are adorned with large sterling silver medallions. (1) Mexico silver slip is adorned with an abalone medallion. (1) Mexico silver clip is adorned with a 1963 One Peso coin. Total weight (excluding Peso coin clip) = 4.35 troy oz.
- You have read and agree with the Auctioneer Terms and Conditions below and the Invaluable Terms of Use Agreement
- You agree to pay a buyer's premium of up to 30% and any applicable taxes and shipping.
Shipping, packing & insurance is at the expense of the buyer. We will assist you in your shipping arrangements for large items & handle some packing and specialized boxing in house- Please email email@example.com for assistance.


text:
The pain value is 29.8700. Obtained this value through iMaxPain on iPhone App Store.
Humm - tell you what hotrocks - post some Mar 30 calls on the market for $3.00 today, and I will buy every one of them. If it then goes below $30, you can put it all in your pocket.


text:
Says Thornburg destroys basis for NCAA sanctions
To the editor:
Your tepid editorial of Feb. 13 about the Thornburg response to the Freeh Report faults it for being "partisan." When has a lawyer's argument not been partisan? The point of a lawyer's argument is to be partisan. Thornburg does not claim to be a judge; he is a lawyer, whose duty is to present his client's case.
You also say that Thornburg's report "makes a few good points." It goes further than that; anybody reading it at www.paterno.com should be convinced that it demolishes the central claim of the Freeh Report – that Joe Paterno purposely conspired with others to withhold evidence regarding Sandusky. That claim was presumably the essential rationale for the NCAA sanctions.
Some people will say that this is a closed matter and that it is time to "move on." People who believe in the good faith of the Penn State football program do not believe that; they will make sure that this matter is not closed until the NCAA sanctions are lifted.
John Brittain


text:
- Contact:
- email@example.com
- Practice Team:
- email@example.com
Hugh has a wide-ranging practice in both private and public law. He is a noted specialist in media and information law including defamation, confidence, privacy and data protection. His practice also includes advisory work and litigation in the freedom of information field.
He is joint author of the leading practitioner texts on the law of human rights and on civil actions against the police. Hugh is highly rated by the directories in libel and civil liberties. He is a regular participant in Council of Europe human rights judicial training seminars.
In addition to his media and human rights work, Hugh is regularly instructed in substantial commercial, professional negligence and construction disputes. He acts as an ICC arbitrator and is an accredited mediator.
'Implications for the Private Sector' in P Carey and M Turle eds, Freedom of Information Handbook (2nd Edn, Law Society, 2008)
Civil Actions Against the Police, with R Clayton (Sweet & Maxwell, 3rd Edition, 2004)
The Law of Human Rights, with R Clayton, (Oxford University Press, 2nd Edition, 2009).
The Law of Personal Privacy (Hart Publishing, forthcoming).
Lenders Claim, with T Grant (Sweet & Maxwell, 2010)
Hugh accepts instructions under the Bar Council Standard Contractual Terms, details of which can be found here.
"He is the fount of all knowledge when it comes to information law, and fun to work with."
"He is a heavyweight in the field of information law."
"He is one of the go-to media silks for claimants and is one of the most influential lawyers in this area."
"He is authoritative, yet realistic. He is innovative and at the top of his game."
Chambers & Partners 2016
Click here to email these CVs to a colleague


text:
BROOKLYN - Residents are lining up to buy Powerball tickets before Saturday night's drawing.
The jackpot is currently at $415 million.
A bodega on Rockaway Parkway in Canarsie saw a steady stream of people buying their tickets in hopes of becoming millionaires.
The winning numbers will be announced at 10:59 p.m. and if no one wins, the jackpot will continue to go up.


text:
Display caption
The engraver and publisher Robert Cromek commissioned this painting from Blake's friend Stothard. Cromek put it on display and charged visitors a shilling to see it. He also collected subscriptions for the forthcoming print of the painting.
Blake claimed that Cromek had commissioned a painting of the Canterbury Pilgrims from him first, but Cromek had not liked the design and so took the commission to Stothard. Blake accused Cromek and, through him, Stothard of copying his long, frieze-like composition. It is unlikely that Blake's accusations were well-founded, but the dispute effectively ended Blake and Stothard's friendship.
September 2004


text:
Category Insight Director
Get similar jobs by email. Register with us now.
|Job Title
|Category Insight Director
|Contract Type
|£50000 - £60000 per annum + Car + Benefits
|Start Date
|Our Reference
|Who to Contact
|Quest Seach Selection - email@example.com
|Job Advertised
|almost 3 years ago
Job Description
A leading market research consultancy is currently recruiting for a Group Insight Director to drive forward key external relationships and ensure clients receive 'best in class' market Insights. The Group Insight Director will be responsible for managing and directing client service activities and increasing client value in order to attain profitable growth and to achieve business goals. The role is sales orientated through the development of strong long term relationship building. The Group Insight Director is responsible for leading contract negotiations with key clients, and the execution of client-specific strategies in line with company policy. The responsibilities for the role also include: * Liaising with central product management, providing feedback to clients, ensuring they maintain their desired revenue, profitability and growth * Promote company externally through client events, conferences and presenting at similar external events * Develop and manage own team, setting clear business objectives and ensuring best-practice techniques are being utilised * Contributing to the company's operational decision making process and maintaining a visible presence throughout all functions To be successful in this role, you shall have excellent project management and strategic planning skills, along with strong business and financial understanding. You will need an analytical mind and experience of working within a Retailer, FMCG company or Research Agency specialising in Retail data and have managed client relationships previously. The ideal candidate shall also have: * Strong communication skills and proven track record of developing senior client relationships * Expert influencing and negotiating skills utilised both externally and internally to ensure your team runs at maximum efficiency and effectiveness. If you feel you have the necessary skills, and want to deliver your Insights to a variety of high profile clients at a company that is held in high regard in the marketplace, then we would welcome your application. We request that candidates send their CV as a Microsoft Word document where possible. Quest Search and Selection is acting as an Employment Agency in relation to this vacancy.
Ref: Q94897 | Published: almost 3 years ago
Similar Jobs
- Digital Insight Manager
£27,000 - £40,000 | Dubai, Dubai
Ref: Q101676 | Published: 26th Oct 2016
I am looking for a Digital Business Executive to work for an international company here in Dubai....
- Digital Insight Manager - Dubai
£35,000 - £40,000 | Dubai, Dubai
Ref: Q101691 | Published: 27th Oct 2016
An exciting opportuntiy exists to join a new company in Dubai that is already established in Euro...
- Regional Commercial Director
£125,000 | London, London
Ref: Q91363 | Published: 25th Oct 2016
Rapidly growing international fashion brand is in the market to appoint a Regional Commercial Dir...
- Brand Marketing Director
£120,000 | London, London
Ref: Q80625 | Published: 12th Oct 2016
This international retail group is looking for a highly motivated Brand Marketing Director to joi...
- Head of Merchandising
£100,000 | United Arab Emirates,
Ref: Q80190 | Published: 04th Oct 2016
This leading global retailer is searching for an ambitious and highly capable head of Merchandisi...


text:
Kindergarten Enrollment Forms
Please print, fill out, and return all required forms. The 2016/2017 enrollment forms will also be available at the Kindergarten Registration on March 1st.
2016/2017 Enrollment Form
Home Language Student Survey (required)
Verification of Chicken Pox (required)
| 2016/2017 Transportation Form (required for 2016/2017)
This required form is for students starting DK or K for the first time in the 2016/2017 school year.
Concussion Awareness Form (required)
Parent Affidavit ˜ Memo of Understanding (required)
Additional Kindergarten Forms
Health Appraisal Form
Parent Input of DK ˜ K Readiness
Click here to return the the Kindergarten Enrollment page.


text:
Moritzburg: scavenger hunt-city tour
Discover Moritzburg a different way with an exciting scavenger hunt and explore in a fun way the city's attractions. Solve puzzles and discover,…
Duration: 4 hours
From $25
per group
DresdenSee all 121 tickets & tours
Want to discover all there is to do in Moritzburg Castle? Click here for a full list.


text:
How to profit from the fall in the ETF for soybeans, Teucrium Soybean (NYSEARCA:SOYB) and when it reverses, the rise in BRF-Brasil Foods (NYSE:BRFS) a Brazilian meat products company.
Cattle farming in central Brazil
As the chart below clearly shows, SOYB has soared as Brasil Foods has soured. That is due to the drought in the Midwest farm belt of the United States. As the share price of SOYB has increased, so has the short position. From June 29, 2012 to July 13, 2012, the short position rose from 12,348 to 24,647, a 99.6% increase.
By contrast, the short position on Brazil Foods is minimal. A short position above 5% is considered troubling for a company. The short position on Brasil Foods is only 0.88%.
SOYB is up 8.33% this month. While recent trading has been bullish, market action reveals technical patterns that portend a share price decline. Up 8.83% for the month and 7.05% for the quarter, Teucrium Soybean is down 4.47% for the last week of trading. Now trading around $26.80, SOYB is very close to its 52-week high of $27.58. As the chart below demonstrates, much of that increase has been since June 1.
At $14.25, Brasil Foods is near its 52-week bottom of $13.61, with recent earnings disappointing the analyst community. Higher prices for soy beans and other feed grains used for livestock cuts into the margins of meat product companies. At present, Brasil Foods' profit margin is just 5.31%. But when soy beans and other grains fall, Brasil Foods' margin will rise.
This will take the share price much higher as sales growth is up by 10.92% on a quarterly basis. For the year, earnings-per-share growth is higher by 71.03%. Next year earnings-per-share growth is expected to rise another 31.03%.
For emerging market traders there are profits waiting from going long Brasil Foods and short Teucrium Soybean. The drought will not last forever and neither will the two stocks respective share price trajectories. The reversal will be lucrative for those positioned accordingly.


text:
Certificate I in Work Education is
specially designed for participants with a disability, or with
significant evidence of a disability or special needs that impact
on their learning. This course's vocational focus gives
participants the basic skills they need to obtain and maintain
employment or undertake further vocational training.
Participants who complete this course
• Develop the key knowledge and skills to
enhance their lifestyle and employment prospects
• Undertake vocational taster activities
and excursions to gain insight into different industries and learn
basic workplace skills
• Undergo career planning and techniques
to search for a job
• Hear from guest speakers from the local
community and industry
• Gain valuable work experience through a
supported work placement
See the Karingal
Training Fee Structure page for more information.
This training is delivered with Victorian and Commonwealth
Government funding. For more information, visit the
Department of Education and Early Childhood
Development website or contact us via the links
Taking a stand against bullying was the talking point at
Karingal Training's Werribee office after a visit from Brodie's Law
founders Damien and Rae Panlock on Tuesday 6 September.
Melbourne's Justin Andrews has put his troubled past behind him
after reconnecting with employment with help from MatchWorks and
Karingal Training.
Karingal Training Werribee student Penny Keomanivong says she's
found the guidance she needed to set her artistic goals and find a
path to success.
Karingal's mental health program, Transcend, will receive a much
needed revamp to their Marshall location this Thursday 25 August
thanks to GMHBA workplace volunteers.
If challenging yourself to six rounds of golf over 15 hours
while raising money for a great cause takes your fancy, then it is
time to sign up for the Karingal Hundred Hole Hike (HHH) 2017.
Karingal will merge with another leading Geelong-based community
service organisation St Laurence following an announcement last
Friday August 5.
The announcement came after a vote by members of both
organisations late last week, with the new entity to be known as
Karingal St Laurence. The two organisations will now spend
time working towards transitioning their businesses into a single
entity. Until this process is completed, Karingal and St
Laurence will continue to operate as separate organisations.
A hands-on learning approach has laid the foundations for a
successful and rewarding partnership between Karingal and Rosebud
Secondary College (RSC).
Students from RSC who are completing their VCAL have selected
Karingal for their student placements over the past decade. They
have gained experience in areas such as horticulture and
hospitality - two quality Karingal programs which have assisted the
students with employability and industry skills. The placements
have also supported students to connect with Karingal clients and
gain a greater understanding of how important community inclusion
is for people with a disability.
Communication - no matter how uncomfortable it is - was a key
learning at a workshop titled 'A Palliative Approach for People
Living with a Disability'.
Karingal partnered with Barwon South Western Region Palliative
Care for the May 31 event, which explored a palliative approach and
Advance Care Planning for people with a disability.
Geelong-based community service organisations Karingal and St
Laurence, confirmed today they are merging following ratification
of special resolutions put to Association Members of both
organisations this week.


text:
Seen a scammer? Been caught out? Let the world know so they don't get burned. Add information if you see one that's already been logged - scammers move quickly and change details often to avoid detection. Details you log here will be in major search engines within an hour or two.Please Like, Share, Tweet etc. to spread the word.
2014-04-29 07:57:31
| You have received a donation of $2m from Mr. Paul White
Wills/Probate Scam
You have received a donation of $2m from Mr. Paul White. Kindly contact Mr. Paul White via email on email@example.com for more details.
|2014-06-03 07:17:01,
Anonymous added:
|Email Address
|firstname.lastname@example.com ⇒
|+44 7031 9699 02 ⇒ Reverse Phone Lookup (by Spokeo


text:
The remake of "Fame" comes out in two days and we are getting our spandex ready. Join us after the jump. … READ MORE »
The remake of "Fame" comes out in two days and we are getting our spandex ready. Join us after the jump. … READ MORE »
Folk legend Mary Travers of Peter, Paul and Mary may have been a revolutionary artist but she always managed to make fighting the Man look pretty damn good, too. She passed away yesterday after an ongoing battle with leukemia, but her particular brand of laid-back cool lives on. … READ MORE »
On this day some 47 years ago, the world lost one hell of a saucy minx. We're talking, of course, about Marilyn Monroe, the buxom blonde icon to end all icons who shagged movie stars, baseball players and presidents alike. (Allegedly, of course.) In an effort to bring out that little bit of Marilyn in… READ MORE »
From "Public Enemies" to the upcoming "Bonnie and Clyde" remake (starring Hillary Duff, blech), one thing is clear: the gun moll look is in. We'll help you get the look without a gun permit. … READ MORE »
We're excited about the scary sweet monsters from Where the Wild Things Are, and surprisingly also inspired by their bold style. Sport a stripey sweater or golden crown, stare down the competition and consider yourself ready for the fall. We weren't the only ones inspired though, check out these pics from a recent British Vogue… READ MORE »
If you aspire to attend an Ivy League school, summer in the Hamptons and wouldn't mind being compared to Blair Waldorf (no, not for one second!), you, my friend, are all about the East Coast prep look. The yacht-hot style has been around for decades, but "Gossip Girl" and the new, utterly repugnant reality show… READ MORE »
Okay so not everyone loves Bruno (or even likes him), but you have to give him props both for his insane newfound mainstream popularity and willingness to make ridiculously bold fashion choices. He's inspired us to live in a world of spandex, fur, sequins, and hot pants … all at once. Despite steadfastly refusing to… READ MORE »
Angelina Jolie may have started the whole blood-wearing trend with that infamous vial of Billy Bob's bodily fluids she used to wear back in the day, but thanks to society's newfound obsession with all things vampire, the trend is now more mainstream, not to mention popular, than ever. People are literally ravenous (ha) for it:… READ MORE »
It's hard to look all darling when you're freaking baking inside a worrisomely hot car and not say, chillaxin' in the breezy Bahamas. Somehow, our style icon-o-the-day, indie fashion maven Zooey Deschanel, makes toasty weather chic look easy and reminds us of cotton candy, photo booths, lounge chairs, and that sweet, sweet patio water mister… READ MORE »


text:
Questions tagged with 'slang'https://www.grammarly.com/answers/questions/tags/slang2016-10-21T00:32:57ZTAKE NOTE x OBSERVATION x P.S. x T/N ?https://www.grammarly.com/answers/questions/77767-take-note-x-observation-x-ps-x-tn/
 A) take note ≠ translator's note

 So, I've seen T/N on some Korean videos and dramas (with English subtitles), it appears suddenly at the top of the screen and explains something that the viewers don't know (it might be a 'pun' in Korean or the range of meanings of some word, maybe the context of the phrase in case etc), however, I was in doubt if it means ''take note'' or ''translator's note''. Actually, translator's note makes more sense here but can I use take note in this situation too?

 B) What's the difference between TAKING NOTE and MAKING NOTE? In which situation I should use each one?

 ↪ written down on Post-it Notes/Sticky notes = taking notes?

 C) MY MAIN POINT: I wrote a comment in a video and, in the end, I wanted to add one last thing, but it isn't entirely connected to the comment

 e.g. ''the interview was funny to watch

 note*: (another famous person) would like to watch it'' -- because he's a fan of his.

 *I wrote ''note'', but I want so replace with another word - OBSERVATION, TAKE NOTE or P.S., which use is more common? (based on this situation)

 ➀ Observation - I wanted to shorten to OBS, but I saw that OBS could mean obviously (internet slang), so I was concerned that people might misunderstand if I use obs

 ➁ Take note could be used here and in the end of a letter? + at the beggining too?

 ➂ P.S. stands for Post Script, I should use with dots, right?

 I know this post is quite long, I'd be grateful if you could answer everything. Correct me if I made any grammar mistake.

 Thanks in advance

asked Jan 29 at 16:38 by Lily , Contributor

1 answer

 1. Translator's note is commonly abbreviated as T.N. (see this and this).

 2. Take note, in its Latin form nota bene, is commonly abbreviated as N.B., N.b., or n.b. (see here).

 3. To take note means "to observe with care or pay close attention to." Example: Take note of this chemical reaction.

 4. To make note is not really idiomatic. You probably had in mind the expression to make a note of, meaning "to write something down as a reminder," or, more figuratively, "to make a mental note of something."

 5. For observation, you can abbreviate it in many ways. How about "obsv." or "observ."?

 6. Yes: P.S., the abbreviation for Latin post scriptum (meaning "written after"), is written with periods. P.S. usually appears at the end of the full text, so it would be approriate in your case.

 7. note*: (another famous person) would like to watch it actually sounds fine to me. P.S. would work, as would take note, but observation probably less so, because I would exepct what follows observation to refer to something that is actually in the text, on which you wish to comment (for our purposes, your video is a "text"). But you are adding extra information, not directly related to the content of what the viewer has just seen. But this is a judgement call.

 8. Adding comments to videos is a very specific enterprise, and it might have its own standards of what is common and what isn't. Indeed, commenting on Korean videos might have different standards from commenting on, say, Hindi videos---for all I know. But in truth, I don't know.

answered Jan 29 at 22:46 by linguisticturn, Expert
 Is it laziness, or simply incorrect?https://www.grammarly.com/answers/questions/72481-is-it-laziness-or-simply-incorrect/
 I'm located in south central Pennsylvania. I've noticed young people in the area speaking in a presumably lazy, and disturbing manner.

 "You want mash with that?" (mashed potatoes)

 "It's all?" (gone)

 "You want to come with?" (me to _____ )

 Please, provide me with some insight so that I can counter this butchery.

asked Oct 22 '15 at 00:02 by Vlad Mikijanic, New member
 Is the word "don't" appropriate to use or is it slang?https://www.grammarly.com/answers/questions/71029-is-the-word-dont-appropriate-to-use-or-is-it-slang/
This morning my s/o and I had a huge disagreement over the use of the word "don't". The statement made was as follows, "we don't want your help". My boyfriend feels the use of the word "don't" is improper and "do not" should be used in its place. I feel he is wrong, as usual. Some help with this one please and thank you.

asked Sep 03 '15 at 20:10 by Colleen, New member

2 answers

 "Don't" is simply a contraction and can absolutely be used in speaking and writing. I was taught (and I am old…) never to use a contraction in formal or academic writing, but many style guides are no longer completely prohibiting contractions. Contractions are acceptable now when they aid the flow of reading or sound less awkward than the long version. I still don't use them often in formal writing, and I would probably advise anyone to avoid their overuse.

answered Sep 04 '15 at 02:36 by Erika McCleese, Expert
 Grammar Correct Written-Slanghttps://www.grammarly.com/answers/questions/13247-grammar-correct-written-slang/
 The common abbreviation/written-slang for 'download' is 'DL' or 'dl'. If one wishes to say 'downloading' or 'downloaded' whilst still using the abbreviation, how would you say it should be written? (This would also apply to other similar abbreviated/written-slang words.)

 A) dling/dled / DLing/DLed

 B) dl'ing/dl'ed / DL'ing/DL'ed

 C) dl-ing/dl-ed / DL-ing/DL-ed

 D) option not listed

asked Mar 12 '13 at 18:30 by TheMadKatter13, New member

2 answers


text:
HCA, Hospital Corporation of America Microbiology Laboratory Asst II PRN in San Jose, California
We're hiring top talent today to join an outstanding Clinical Laboratory Team that strive for excellence in servicing our patients at San Jose Regional Medical Center. Interested in joining this team? Apply online now.
Schedule/Shift: PRN - Per Diem (as needed)
Internal Posting Period: 8/8/16 - 8/15/16
_POSITION SUMMARY_: Handles and prepares multiple specimen types for culture setup. Performs routine maintenance duties such as monitoring equipment, preparing reagents, reviewing specimen logs, answering calls for specimen collection and results.
_LICENSE/CERTIFICATION REQUIREMENTS_: Minimum: None Preferred:
_EXPERIENCE REQUIREMENTS_: Minimum: Ability to multi-task and strong attention to detail. Excellent interpersonal & Customer Service phone skills, tact with understanding with patients, physicians, public and medical center personnel. Excellent communication skills, Pay close intention of detail. Ability to handle multiple tasks. Exercise good judgment & common sense Preferred:
_EDUCATION REQUIREMENTS_: Minimum: High School Diploma or Equivalent. Some college or vocational level science (Chemistry and/or Biology) and/or laboratory classes Preferred:
_KNOWLEDGE/SKILLS_: Excellent verbal and written communication skills, problem solving, critical thinking ability, Able to read doctor's orders & patient info on specimens. Able to write neatly in order to label specimens, document issues/problems and to maintain logs of specimens received Able to perform non-complex arithmetic calculations in order to mix laboratory solutions. Basic chemistry/biology knowledge. . Familiarity with basic laboratory terminology.
Title: Microbiology Laboratory Asst II PRN
Location: California-San Jose-Regional Medical Center of San Jose
Requisition ID: 08385-16352


text:
Corri, uomo, corri (Run, Man, Run) (1968)
Critic Consensus: No consensus yet.
Corri, uomo, corri (Run, Man, Run) Videos
Corri, uomo, corri (Run, Man, Run) Photos
Movie Info
Watch it now
as Santillana
as Dolores
as Manuel 'Cuchillo' Sanchez
as Michel
as Penny Bannington
as Nathaniel Cassidy
as Riza
as The Mayor
as Ramirez
Critic Reviews for Corri, uomo, corri (Run, Man, Run)
All Critics (1) | Fresh (0) | Rotten (1) | DVD (1)
Even if Van Cleef is a one-dimensional actor, he nevertheless has a tremendous screen presence.
Audience Reviews for Corri, uomo, corri (Run, Man, Run)
In my opinion, this sequel to The Big Gundown is a lot better than its predecessor. Not only does this feature more of the Italian superstar known as Tomas Milian, it has a better thought out plot and set of characters. It also has no problem being completely trashy; such as the mudfight between two bombshells. It has a lot more heart than it needs to, but also delivers on the violence and gore expected of a Spaghetti Western. A really like the whole Mexican Revolution involvement in this, it sort've just plays in the background as a frame of reference.
Super Reviewer
I wasn't sure what to expect. A second or even third tier suckfest, really. I couldn't have been more surprised. Yeah, it suffers from some bad acting, and the immemorable lead characters are disappointing, but it was entertaining and worth watching for anybody who enjoys westerns, especially the spaghetti kind.
not quite in the pantheon on spaghetti classics, but only a slight notch down. its in the realm of the great silence and stuff like that. definitely a must see for spaghetti western fans.
Corri, uomo, corri (Run, Man, Run) Quotes
There are no approved quotes yet for this movie.


text: Summer is booking up quickly. Make sure you have the best photography for your wedding of your event of any type. For new/prospective clients, my Facebook page has even more photos of people livin', lovin' and laughin'. Stop by and be sure to take a look at the reviews. https://www.facebook.com/JohnKingPhotography/ While you're there go ahead and click that 'like' button.


text:
South Kihei (Kamaole I & II Beaches Area)
- Kamaole Beach Club
- Kamaole Beach Royale
- Kamaole Nalu
- Kamaole One
- Other So Kihei (Kamaole I&II Area) Properties
This property allows you to book online with a credit card. After submitting a reservation request, the owner or manager has 24 hours to accept, at which time your card will be charged and you will receive an email confirmation
Recent South Kihei (Kamaole I & II Beaches Area) reviews
"This condo was perfect for our vacation needs. It was fully stocked with everything you could possibly need - beach chairs with handy carry strap, coolers, boogie boards, laundry and dish detergent, and a smattering of cooking spices. The kitchen was very well stocked, and helped for a budget friendly stay (and also when we were tired of eating out!). The location was our favorite on the island, and we stayed in Hana, Kaanapail and Napili also on this trip. My 11 year old son rated this place the highest over our fancy hotel in Kaanapali, because he said there was so much variety of things to do here. He liked the park across the way, the beach across the way for boogie boarding, and being able to walk to so many things. Kihei is great because it's centrally located, and you can get to everywhere from here. The beach out front was perfect for trying out paddle boarding and for surf lessons. Roll out of bed and walk on over! We have not been in Kihei for a while and were surprised to see all of the new restaurants (plus old favorites). There are a ton of inexpensive (for Hawaii) options nearby that were really good and did not break the budget. This is a wonderful place to stay if you want to get away from all of the artificial "Hawaii" in Kaanapali and enjoy the real thing."Guest: Andrea D. Date of Stay: July 2016 Review Submitted: July 30, 2016View listing
"Very clean condo with all the comforts of home. Conveniently located near the airport, nice beaches, restaurants and shopping. When the wind comes up in the late morning/early afternoon at the beach, the pool area(s) are a great place to retreat."Guest: Bethany R. Date of Stay: October 2016 Review Submitted: October 17, 2016View listing
"We had a wonderful stay at Klassy Kalama. The property is clean. It is close to everything in Kihei. Jerry and Meredith were very welcoming, responsive and patience with all of my queries. The booking process from start to finish was a breeze and we were provided with clear instructions and directions. The condo has everything you need. We have travelled to other Hawaiian islands and always stayed at places that have full kitchen because we loved getting local produce and seafood from the local markets to fix our meal. So, the first thing that I did was checked out the kitchen. I must say Klassy Kalama's kitchen is exceptional! The kitchen appliances works great, the utensils and dishes were clean. There were various sizes of pots and non stick pans, as well as various kinds of knives. All were good quality. The cabinets were well stocked with all kinds of condiments, herbs and spices, etc, and everything was well organized. And...I'm a fan of glass cookware :) As soon as I saw them in the kitchen cabinets, I was ecstatic! I knew then that I would enjoy cooking in this kitchen, just like at home! The condo also has everything you need for beach day. We used the beach chairs, beach mats, umbrella, cooler, beach towels, snorkel sets and boogie boards. We really like Kihei. In my opinion it has more of a campy beach town feel to it compared to West Maui and you are around MANY more locals, which is perfect for us! Great location as starting point to drive around the island. We spent a lot of time on the beach and definitely appreciate that this condo is walking distance to many beautiful beaches in South Maui. Our favorites are Charley Young and Keawakapu Beach. They are family friendly beaches. Sunsets are beautiful at any of these beaches. Charley Young is 5 mins walk from the condo and is a turtle highway!! Some of them swam right up to us when we were swimming. The waves were great for boogie boarding. Our son said, "I hope Jerry will keep this boogie board forever so that it will always be here when we come back!" The complex is well maintained. They were installing a gazebo in the BBQ area the day we left. Jerry and Meredith were easily contacted when we were at the condo and readily to assist. We certainly appreciate their professionalism. From the Haleakala's peak to underwater Molokini -- MAHALO, Jerry and Meredith! Klassy Kalama was our home away from home. We hope you are able to receive us again soon! Maybe Molokai next :)"Guest: Y B. Date of Stay: August 2016 Review Submitted: August 31, 2016View listing
"Kihei Akahi unit C618 - October 2016 We are back in Canada after having a great stay in Maui. We really appreciated the nice touches found around the unit – the reno'd bath and kitchen, great art work everywhere, comfortable furniture, great king bed, even the pineapple desk lamps were a nice touch. Location is great being in the middle of Kihei, walking distance to most things. Everything is in the unit that you might need for a stay - beach umbrella, towels and chairs, snorkel gear, boogie boards, and a raft of other items. We had an issue with a loud A/C unit. As soon as we mentioned it to Kathy, it was replaced the following day! That's great service. Being up on the 6th floor (yes, there is an elevator), there was usually a breeze that was able to keep the unit pleasant. In the summer, the A/C probably wouldn't be able to keep the unit and especially the bedroom from being quite warm. For us in October, it was pleasant to warm. There are extra fans that can be used to keep cooler. Wifi, was limited in the unit, however this was also fixed fast when we were supplied with another router to connect to. Kathy is obviously a great host and kept us happy. Communication was great and she even allowed us to check out late at no charge."Guest: Jeff T. Date of Stay: October 2016 Review Submitted: October 23, 2016View listing


text:
*** Free delivery ***
You have no items in your shopping basket.
New to Steffans? Sign up.
Create an account for future convenience, fast checkout and create a wishlist.
You don't yet have any items in your wishlist.
Please login or register for an account.
ChloBo Double Wing Charm
Interchangeable 925 Sterling Silver Double Wing Necklace Charm.
Angel Wings are often worn to symbolise aspirations to reach greater heights.
Product Reference: PEMWINGS
© Copyright 2016 Steffans Jewellers.


text:
According to our records, Robert J. Bach is possibly single.
Help us build our profile of Robert J. Bach!
to add information, pictures and relationships, join in discussions and get credit for your contributions.
Robert J. Bach (born December 31, 1961), commonly known as Robbie Bach, was the President of Entertainment & Devices Division at Microsoft. He led the division that is responsible for the Xbox, Xbox 360, Zune, Windows Games, Windows Mobile and the Microsoft TV platform. After 22 years at Microsoft, Robert announced his retirement from Microsoft effective in the fall of 2010. Robert now speaks to corporate, academic and civic groups across the country and in 2015 completed his first book, Xbox Revisited: A Game Plan for Corporate and Civic Renewal.
Help keep Robert J. Bach profile up to date.
Connect any celebrity with Robert J. Bach to see how closely they are linked... romantically!


text:
SS&C Bolsters CRM Capabilities With Acquisition of Salentica
Salentica's expertise lies in wealth management CRM implementations for such clients as investment advisers, broker-dealers, institutional wealth managers and trust companies.
12 Pioneering IoT And Virtual Reality Startups Getting Investments From Intel Capital
Intel's global investment organization has recently invested $38 million in a dozen Internet of Things and virtual reality startups. Here are the companies with new funding.
Head-To-Head: New MacBook Pro Vs. MacBook Pro 2015
The CRN Test Center lays out how Apple's new MacBook Pro measures up against the previous generation.
First Look At Apple's Three New MacBook Pro Versions
Apple Thursday debuted the biggest overhaul to the MacBook Pro in years. Here are the key things to know about the updated versions.
Coverage From BoB Conference 2016
The Best of Breed Conference 2016 gathers industry executives from CRN's Solution Provider 500, Tech Elite 250, Next-Gen 250, Fast Growth 150 and Managed Service Provider 500 communities to examine the future of the channel.


text:
Rory Gallagher: Belfast statue of rock legend gets approval - BBC News
by BBC Entertainment
3 days ago
Read Full Post
by BBC Entertainment
3 days ago
23 minutes ago
Kittens that are weaned too early may develop physical and behavioral issues later in life.
36 minutes ago
Strictly Come Dancing bosses have denied reports that a mystery bug has struck down six of its professional dancers.
1 hour ago
FIFI Box is best known in Australia as a radio host. But that might be about to change, as she embarks on a new phase of her career — as star of Neighbours.
1 hour ago
THE fact that it's still remarkable for a woman to get a big, meaty acting role shows there are still too few on offer, Deborra-Lee Furness believes.
1 hour ago
'Real American Places: Edward Weston and Leaves of Grass' features black-and-white photographs taken during the artist's eight-month journey for a deluxe printing of Walt Whitman's poetry.
1 hour ago
Wanted: a Google map that pinpoints the locations of lyrics across Southern California. Thee Midniters celebrated Whittier Boulevard in their song of the same name. Tom Waits switched up the intersection of Hollywood and Vine to create his great ode to t
3 hours ago
Mexican graffiti artists have painted a stretch of the US-Mexico border fence to promote peace.
3 hours ago
ARIEL Winter relied on her Modern Family to embrace her curves.
4 hours ago
As the release date of 'Ae Dil Hai Mushkil' is inching closer, more and more Bollywood insiders are coming out in the open to support the Karan Johar film that is stuck amidst major controversy.
4 hours ago
Critics Rating: 2.5 stars, click to give your rating/review,My Father Iqbal can pass of as a story of any regular person caught in tumultuous times but the J&K


text:
3 Reviews
A nice side for our family barbecue. Making 24 hours ahead allows the flavors to mellow. Made almost as written the only change I made was not measuring the yellow squash or zucchini just used one whole one of each which was more like a cup of each. Thanks so much for the post.
I really enjoyed this dish!
Very good but different salad which we enjoyed with broiled fish. Thanks for sharing!


text:
Let's log you in.
Don't have a StudySoup account? Create one here!
Create a StudySoup account
Be part of our community, it's free to join!
Already have a StudySoup account? Login here
Unit 4
Unit 4 BIO 204
MiraCosta College
Popular in Metabolic Biochemistry
Popular in Biology
This 12 page Study Guide was uploaded by David Edwards on Monday May 16, 2016. The Study Guide belongs to BIO 204 at MiraCosta College taught by S. Bailey in Spring 2016. Since its upload, it has received 83 views. For similar materials see Metabolic Biochemistry in Biology at MiraCosta College.
Similar to BIO 204 at MiraCosta College
Popular in Biology
Reviews for Unit 4
Report this Material
What is Karma?
Karma is the currency of StudySoup.
You can buy or earn more Karma at anytime and redeem it for class notes, study guides, flashcards, and more!
Date Created: 05/16/16
Replication laying down new nucleotides causing an incorrect pairing The process through which complimentary to the template that could lead to point the genetic information of a strand on both the leading and mutations due to DNA's lagging strand after the RNA semiconservative replication cell is copied through a series of enzymatic reactions to pass primer has been established properties identical DNA to daughter o alphasubunit: Enzymatic Telomere cells. polymerization of DNA in Repetitive DNA added to the 5' to 3' Polarity 3' ends of chromosomes early the 5' 3' direction refers to the carbon numbers o epsilonsubunit: in development of the sugars ribose and Exonuclease that proofreads End replication problem deoxyribose which contain in a 3'5' direction occurs because lagging hydroxyl groups to which strands would be staggered o betasubunit: holds the leaving them uncopied incoming nucleotides are whole complex onto the attached during DNA strand Telomeres prevent the polymerization, with addition o tausubunit: Tethers the two staggered ends from specifically to the 3' hydroxyl alpha subunits of DNA activating the cells systems group, resulting in increases for monitoring damaged in length at the 3' end, in polymerase III to each other DNA. DNA Polymerase I polymerization in the 5' to 3' Replaces the RNA primer Also acts as a buffer that direction with DNA after RNase provides protection against Antiparallel the organism's genes The configuration of a DNA degrades the RNA primer. shortening. They do get Polymerizes in the 5'3' molecule that is made up of direction shorter after every round of two strands held together by DNA Ligase replication eventually leading complimentary base pairing. to somatic cell apoptosis or This results in one strand An enzyme that connects the senescence= no more cell reading 5'3' one direction fragments of lagging strand division. DNA and 5'3' in the opposite Okazaki Fragment Telomerase direction. The enzyme that adds RNA Primase Lagging strand segments that telomeres to DNA strands. An enzyme that lays down contain the RNA primer and Operon nucleotides laid down by complimentary RNA DNA Polymerase III Set of genes that are related nucleotides to the template Leading Strand and coordinate functionally strand of DNA known as for a protein RNA primers. DNA synthesized Includes the switch segment Necessary before DNA continuously 5'3' following the replication fork (helicase) of DNA called an operator, Polymerase III can lay down Lagging Strand the promoter sequence and the new DNA genes they control. Helicase DNA synthesized in segments Polycistronic The enzyme that "unzips" the away from the replication fork also in the 5'3' direction Describing a type of original DNA strand to start Exonuclease messenger RNA that can replication and create encode more than one replication forks and two An enzyme that works by polypeptide separately within template strands cleaving nucleotides one at a the same RNA molecule. Single strand binding protein time from the end (exo) of a polynucleotide chain. Monocistronic These proteins bind to the Endonuclease An RNA molecule that unpaired, separated DNA encodes for only one protein strands and keep them from An enzyme that works by Promoter repairing. cleaving nucleotides one at a time from the The DNA sequence where Topoisomerase Tautomers RNA polymerase attaches and The enzyme that stays ahead initiates transcription. of the replication fork Rare forms of nucleotides that RNA Polymerase relieving the strain on the yet can "confuse" DNA to be separated DNA because polymerase when the enzyme An enzyme that pries the two selects nucleotides to pair up strands of DNA apart and the untwisting of the double joins together RNA helix causes strain. Nucleotides have a floating nucleotides complementary to DNA Polymerase III proton that can give the the DNA template strand. The enzyme that begins molecule a different structure Assemble a polynucleotide in the 5'3' direction. for translation. Small nuclear RNA that, TFIID/TBP Poly A Tail together with proteins, make up spliceosomes TATA binding protein that At the 3' end, Poly A recognizes the promoter polymerase adds about 200 miRNA sequence and recruits general more adenine nucleotide Micro RNA that regulates the transcription factors to bind Used to facilitate the export of expression of genes by and assemble the mRNA from the nucleus, interacting directly with DNA TFIIH used to protect the mRNA or w mRNA copy A helicase enzyme that from degradation by Capable of binding to unwinds parent DNA and exonucleases, and they help complementary sequences in phosphorylates RNA ribosomes identify and attach mRNA molecules. to the 5' end of the mRNA. polymerase II at the C Allows complex to bind to terminal domain 5' Cap any mRNA molecule with at Phosphates become binding A modified form of guanine least 7 or 8 nucleotides of sites for "hitchhiker" proteins nucleotide added to the 5' end complementary sequence. such as Capping Enzymes and after transcription miRNA and protein complex Cleavage factors Done for same reasons as either degrades the target Also used as a promoter PolyA tail. (Export of mRNA or blocks its clearance nucleus, prevent degradation, translation TATA Box point of attachment for Ribozyme A nucleotide sequence ribosomes) RNA molecules that function containing TATA, about 25 Exon as enzymes nucleotides upstream from the Regions of mRNA that are found in the ribosome where transcriptional start point. expressed by being translated they join amino acids together Template Strand into AAs. to form protein chains The strand of DNA that is Intron 5'/3' UTRs used to make a new strand of Regions of mRNA that are Parts of the mRNA that will RNA during transcription by noncoding segments that are not be translated into protein, matching complimentary usually between coding but they have other functions, nucleotides to it. regions and are cut out by such as ribosome binding. Nontemplate Strand large complex of proteins and Start codon The other strand of DNA not snRNA called splicesomes. Codon AUG that signals the being used to create RNA snRNP proteinsynthesizing during transcription A complex of snRNA and machinery to begin translating Cterminal Domain proteins that bind to introns to the mRNA at that location. The carboxyl end of an amino splice them out of mRNA Stop codon acid where more amino acids Spliceosome Termination codon that are attached to create the The formal name of the signals the end of a growing polypeptide chain complex made up of snRNA translation. during translation and binding proteins that Aminoacyl tRNA Synthetase General Transcription Factor cleave out introns from (AARS) class of protein transcription mRNA Correct matching up of tRNA factors that bind to specific mRNA and amino acid is carrier out sites (promoter) on DNA to Molecule of nucleotides that by these enzymes. activate transcription of is transcribed from DNA and Active site of each AARS fits genetic information from translated into AAs via only a specific combo of AA DNA to mRNA. ribosomes. and tRNA making there be 20 Poly A Signal tRNA different synthases for each Polyadenylation signal Transfer RNA used to transfer AA. sequence in DNA (TTATTT) amino acids from the Catalyzes the covalent that specifies a signal cytoplasmic pool to a growing attachment of the AA to sequence in mRNA polypeptide in a ribosome. tRNA driven by hydrolysis of (AAUAAA). rRNA ATP Once this stretch of RNA molecules that, together Ribosomal Subunit nucleotides appears, it is with proteins, make up Small subunit immediately bound by ribosomes; the most abundant o Binds to both mRNA and a cleavage factors to release the type of RNA mRNA to head to ribosomes specific initiator tRNA, snRNA which carriers the AA polymerase to the promoter Activate or inhibit gene methionine. Binds the preventing transcription of the expression by recruiting mRNA at a specific RNA genes. chromatin modifying enzymes sequence, just upstream Corepressor to genes. from the start codon, AUG Small molecule that Combinatorial Code o Binds to the 5' cap of the cooperates with a repressor Only a dozen or so nucleotide mRNA and then moves, or protein to switch an operon sequences appear in control scans, downstream along off elements for different genes. the mRNA until it reaches Inducer Each enhancer is composed of the start codon Small molecule that about ten control elements Large subunit inactivates the repressor if the each of which can bind only o Completes the initiation one or two specific tx factors. operon is always active and complex. requires a repressor to turn it The particular combination of o Hydrolysis of GTP off. control elements in an provides the energy for the Catabolite Activator Protein enhancer associated with a assembly. Initiator tRNA agene, rather than the is in the P site; the A site is (CAP) A protein (activator) that presence of a single unique available to the tRNA with binds to DNA and stimulates control element that is the next AA transcription of a gene. important in regulating transcription. "senses" the transport of Histone Acetyltransferase (HAT) another sugar and binds to site Initiation Factors near promoter and interacts Modify chromatin so its more Proteins that are required to with RNA polymerase, open and RNA bring all the translation stabilizes it on a weak polymerase/transcription factors can bind easier and components together. promoter. Elongation Factors o Promoters have specific transcribe a gene. Several proteins that are sequences of nucleotides Histone Deacetylase (HDAC) required to add AAs one by and if sequence doesn't Remove functional group match ideal sequence of from histone tail causing a one to the previous amino acid at the Cterminus of the promoter, it will not recruit highly condensed chromatin growing polypeptide chain. RNA polymerase well state. lac operon cAMP Methyltransferase A protein that accumulates histonemodifying enzymes Set of genes that are related and coordinate functionally when glucose is scarce that that catalyze the transfer of for lactose allosterically regulates by methyl groups to histone o Lac Z: encodes beta binding to CAP protein. proteins Enhancer gene is brought into a closed galactosidase which cleaves and begin Distal control element which chromatin state so it may not metabolism of lactose maybe thousands of be expressed such as silencing o Lac Y: encodes lactose nucleotides upstream or pancreatic cell from downstream of a gene or even expressing the sequence for permease which is a hemoglobin production. membrane transport within an intron. protein Activator proteins bind to the Heterochromatin o Lac A: encodes enhancer area which has three also referred to as closed galactosidase binding sites. DNA bends via chromatin or gene poor bending proteins that bring because it is inaccessible to transacetylase which is still not understood activators closer to the the machinery of the cell Operator promoter. General responsible for transcribing Positioned within the transcription factors, mediator the genetic information coded proteins, and RNA in the DNA promoter, or between polymerase II are nearby. promoter and enzymecoding Euchromatin genes. Activators bind to certain true chromatin is loosely Controls the access of RNA mediator proteins and general packed making its DNA tx factors helping them form accessible to machinery so the polymerase to the genes. an active transcription genes present in euchromatin Repressor A protein that binds to the initiation complex on the can be transcribed. operator and blocks promoter. Maternal Effect Gene Regulatory Transcription Factor these genes specify polarity of attachment of RNA a developing cell into anterior, tx factors with conserved nurse cell to oocyte and posterior, dorsal, and ventral. DNA binding domains localized to opposite end to Expressed by mother during (homeodomains) become posterior of organism oogenesis (egg development) Similar sequence amongst Bicoid and Nanos mRNA are many organism and they localized at opposite poles of control pattern formation in the oocyte. the late embryo, larva, and adult Segmentation Gene Established by the maternal Bicoid effect gene Gene that is expressed and Determine the position and mRNA is transported from nurse cell to oocyte and polarity of each segment of a developing organism created specifically localized to the by morphogen concentrations region in contact with the that encode for transcription nurse cells to become the factors anterior of the organism Homeotic (HOX) Gene Nanos Homeobox genes encode for Gene that is expressed and mRNA is transported from 1. Where does the energy for the synthesis of nucleic acid molecules come from? How does this necessarily constrain the direction of synthesis of new nucleic acid strands? The linkage of nucleic acid molecules is a dehydration synthesis reaction. Adjacent nucleotides are joined by a phosphodiester linkage consisting of a phosphate group that links the sugars of two nucleotides. The bond results in a repeating patter of sugarphosphate units called the backbone. The two free ends of the polymer are different. One has a phosphate attached to a 5' carbon and tother end has a hydroxyl group on the 3' carbon. This restricts a nucleic acid polymer to only be able to add more sugarphosphate molecules to the 3' end of the previous sugarphosphate structure because during hydrolysis, a water molecule is added and the Oxygen molecule leaves the hydroxyl on the 3' carbon the oxygen on the phosphate group can bind to the carbon 2. How did the MeselsenStahl experiment provide support for the semiconservative model of DNA replication? How would the results have been different if the mode of DNA replication were conservative? Their experiment eliminated the conservative model for DNA because the first replication produced a band of hybrid DNA. The second replication produced both light and hybrid DNA, a result that refuted the dispersive model and supported the semiconservative model. This proves that unlike a conservative model, daughter cells will contain one old strand from the parent molecule and a newly made strand. A conservative model would have the two parental strands somehow come back together after the process and be seen in its entirety in the daughter cells. 3. What are the specific functions of E. coli DNA Polymerases I and III in DNA Replication? DNA Polymerase III adds DNA nucleotides to an RNA primer using the parental strand as a template to add complimentary nucleotides. DNA Polymerase I replaces the RNA nucleotides of the primer after RNase degrades the primer. 4. Define the functions of the alpha, epsilon, beta and tau subunits of E. coli DNA Polymerase III during replication. Alpha: the enzymatic polymerization of DNA in the 5'3' direction Epsilon: exonuclease 3'5' that acts as a proofreader to ensure nucleotides are complimentarily paired Beta: The subunit that clamps polymerase to the DNA strands Tau: Tethers two parental strands and the polymerase to one another. 5. What is an exonuclease? How do 5' to 3' and 3' to 5' exonucleases differ from one another functionally? How does an endonuclease differ from an exonuclease? An exonuclease is an enzyme that degrades nucleotides from the one end of the molecule which is different than an endonuclease which degrades nucleotide molecules from with in the molecule. The difference is on which strand the exonuclease is acting on. DNA polymerase III, for example, proofreads the template strand in the 3'5' direction but lays new nucleotides in the 5'3' direction. RNase acts as an exonuclease, deleting RNA primer in the 5'3' direction on the leading/lagging strands. 6. Define the roles of each of the following in DNA replication: Helicase, DNA Polymerase I, DNA Polymerase III, Primase, Single Strand Binding Protein, Topoisomerase, DNA Ligase, RNAse. Helicase: unwinds parental double helix at the replication forks DNA Polymerase I: replaces the RNA nucleotides of the RNA primer with DNA nucleotides. DNA Polymerase III: adds new DNA nucleotides to the RNA primer that must first be laid down Single Strand Binding Proteins: Binds to and stabilizes singlestranded DNA until it is used as a template to prevent the just unwound helix from coming back together Topoisomerase: Relieves overwinding strain ahead of replication forks by breaking, swiveling, and rejoining DNA strands DNA Ligase: Joins okazaki fragments of lagging strand once the RNA primer is replaced with DNA nucleotides. On the leading strand, it joins the 3' end of DNA that replaces primer to the rest o the leading strand of DNA RNase: an exonuclease that degrades the RNA primer before DNA polymerase I can replace the nucleotides 7. What's the difference between the leading strand and the lagging strand? Why is it necessarily true that every replication bubble will have two leading strands and two lagging strands? The difference between the leading strand and the lagging strand is that the leading strand is continuously formed once the primer is laid down, DNA polymerase III moves towards the replication fork adding nucleotides. The lagging strand must wait as the double helix is unzipped because nucleotides can only be added on the 3' end causing the DNA polymerase III to add nucleotides segment by segment in the opposite direction of the replication fork. There are two of each strand because replication occurs in a bidirectional format from the origin of replication creating two replication forks. 8. Although both catalyze new covalent bond synthesis in DNA, DNA Polymerase requires no ATP for energy, whereas DNA ligase does require the energy of ATP. Explain why this is so. This is because hydrolysis can drive the connection of new nucleotides to a growing strand by DNA polymerase but to connect a sugarphosphate backbone of a 3' carbon to a 5' carbon the the strand "in front" of it requires ATP to create the new bond. 9. Define the end replication problem in eukaryotes, and explain how eukaryotic cells deal with the problem. Replication machinery cannot complete the 5' ends of daughter DNA strands because a DNA polymerase can add nucleotides only to a 3' end of a preexisting polynucleotide. Once a primer is removed from the last fragment there is no 3' end for new nucleotides to be added so repeated rounds of replication produce shorter and shorter DNA molecules with staggered ends. Eukaryotic chromosomal DNA molecules have special sequences called telomeres at the end that do not contain genes but multiple repetitions of one short nucleotide sequence. Telomeres prevent the cell from activating mechanisms to handle damaged DNA such as cell cycle arrest or apoptosis. Also the telomeric DNA acts as a buffer so the DNA does not shorten too fast. An enzyme called telomerase catalyzes the lengthening of telomeres in eukaryotic germ cells, thus restoring their original length and compensating for the shortening that occurs during DNA replication. 10. What is the Hayflick limit? How is it related to telomeres, cellular aging and senescence? The Hayflick limit is the number of times a normal human cell population will divide until cell division stops This is related to telomeres, cellular ageing, and senescence in that telomeric DNA tends to be shorter in dividing somatic cells of older individuals and in cultured cells that have divided many times. It has been proposed that shortening of telomeres is somehow connected to the ageing process of certain tissues and even to aging of the organism as a whole. Once a telomere is too short the cell will be signaled for apoptosis or senescence which is no more cell division leading to aging tissues/aging organism. 11. What is meant by the terms deamination and depurination? How does the cell "deal with" each of these problems? Deamination is the removal of an amine group from a molecule. Enzymes that catalyze this reaction are called deaminases. In DNA, deamination of cytosine is corrected by the removal of uracil. This site is then recognized by endonucleases that break a phosphodiester bond in the DNA, permitting the repair of the resulting lesion by replacement with another cytosine. A DNA Polymerase may perform this replacement by its 5'>3' exonuclease activity, followed by a fillin reaction by its polymerase activity. DNA ligase then forms a phosphodiester bond to seal the resulting nicked duplex product, which now includes a new, correct cytosine. This is done is similar fashion for all nucleotides Depurination occurs when a purine (A or G) is released from a sequence. The cell can repair this with an endonuclease that cuts a segment of DNA and then DNA polymerase rewrites the sequence to insert the missing base pair. 12. What is an intercalating agent? How do intercalating agents lead to mutation and DNA damage? Such as ethidium bromide and proflavine, are molecules that may insert between bases in DNA, causing frameshift mutation during replication. Frameshift mutation can lead to cancer cell growth or cause the cell to create proteins in incorrect order once transcribed to RNA and translated by ribosomes 13. How does exposure to ultraviolet radiation lead to DNA damage and potentially to mutations? UV is a physical mutagen known as mutagenic radiation which can cause disruptive thymine dimers in DNA. This new structure no longer hydrogenbonds to adenine, and creates a noticeable kink that will signal a base mismatch. If left to its own devices, DNA polymerase will "idle" at this site. The other side can be replicated, leaving a gap at the dimer. This would be lethal in the next round of replication, because it would produce a doublestranded break. 14. Define the four essential steps that excision repair pathways in both prokaryotic and eukaryotic cells have in common. Teams of enzymes detect and repair damaged DNA, such as a thyamine dimer (caused by UV radiation) which can distort a DNA molecule. A nuclease enzyme cuts the damaged DNA strand at two points, and the damaged section is removed. Repair, synthesis by a DNA polymerase fills in the missing nucleotides. DNA ligase seals the free end of the new DNA to the old DNA, making the strand complete. 15. Why is cancer the logical consequence of damage to DNA repair mechanisms like the UV Repair pathway? Skin cancer can be caused by an inherited defect in a nucleotide excision repair enzyme because this can cause mutations to go unnoticed by the cell. The formation of thyamine dimers like those in UV damage caused DNA to buckle and interfere with DNA replication and if these go uncorrected, skin cancer can result. 16. How do some chemotherapeutic strategies exploit defective DNA repair mechanisms in cancer cells? Some chemotherapeutic strategies target the DNA repair and replication mechanisms in cancerous cells to prevent them from repairing DNA that has mutations and continuing to replicate it once repaired. Many cancerous cells do not have a replication limit and they will continue to divide and move mutated DNA to new cells spreading cancer. 17. Compare the enzyme responsible for transcription to the enzyme responsible for initiating each new nucleic acid strand in replication and the enzyme that does the bulk of DNA replication. How are the enzymatic activities similar? How are they distinct? RNA Polymerase II and DNA Polymerase III are similar in that they both read template DNA in the 5'3' direction. The enzymes are different in that RNA Pol can initiate new nucleic acids whereas DNA Pol must have an RNA primer laid down first. RNA Pol also requires general transcription factors to initiate the transcription of mRNA. 18. What is the relationship between the sequence of the transcribed RNA molecule and the template and nontemplate strand of the gene? The mRNA molecule that is transcribed comes from an RNA polymerase II enzyme reading the template strand and producing a complimentary strand. The nontemplate strand is inside the RNA polymerase enzyme, however, it is not read to create an mRNA strand. 19. During transcription, where does the energy for the synthesis of the RNA molecule come from? How does this necessarily constrain the direction of synthesis of the transcribed RNA? How is this related to the synthesis of DNA during replication? This is the same as the construction of a new DNA molecule during replication in that nucleotides are added to the 3' end of the RNA molecule via hydrolysis reactions. Only sugarphosphate backbone molecules can be attached at the 3' carbon of the last molecule. 20. What is meant by coding versus noncoding RNA? What classes of RNA fall into each category and what are their major functions? Coding RNA refers to triplet nucleotide sequences that will code for a protein. Noncoding RNA consist of nucleotide sequences that will not be translated into proteins but have other important functions. mRNA: (coding) strand that is read by ribosome known as "messenger" RNA. Molecule of nucleotides that is transcribed from DNA and translated into AAs via ribosomes. tRNA (noncoding) Transfer RNA used to transfer amino acids from the cytoplasmic pool to a growing polypeptide in a ribosome. rRNA (noncoding) RNA molecules that, together with proteins, make up ribosomes; the most abundant type of RNA snRNA (noncoding) Small nuclear RNA that, together with proteins, make up spliceosomes miRNA (noncoding) Micro RNA that regulates the expression of genes by interacting directly with DNA or w mRNA copy. Capable of binding to complementary sequences in mRNA molecules. Allows complex to bind to any mRNA molecule with at least 7 or 8 nucleotides of complementary sequence. miRNA and protein complex either degrades the target mRNA or blocks its translation 21. What is meant by the term ribozyme? What classes of RNA are categorized as such and what are their major functions? Ribozymes are RNA molecules that function as enzymes. Intron RNA functions as a ribozyme and catalyzes its own excision. (rRNA) RNA is singlestranded, a region of an RNA molecule may basepair, in an antiparallel arrangement, with a complementary region elsewhere in the same molecule; this gives the molecule a particular 3D structure. Specific structure is essential to the catalytic function of ribozymes, just as it is for enzymatic proteins. Some of the bases in RNA contain functional groups that can participate in catalysis. Acid molecules add specificity to its catalytic activity 22. What distinguishes eukaryotic RNA Polymerase II from Pol I, III, IV and V? RNA polymerase I synthesizes a prerRNA which matures and will form the major RNA sections of the ribosome. RNA polymerase II synthesizes precursors premRNA. It controls transcription for the most part and a range of transcription factors are required for its binding to promoters. RNA polymerase III synthesizes tRNAs, rRNA and small RNAs found in the nucleus and cytosol. RNA polymerase IV synthesizes siRNA in plants. RNA polymerase V synthesizes RNAs involved in siRNAdirected heterochromatin formation in plants. 23. Why are general transcription factors required for transcription by Pol II? What are the major functions of the two general transcription factors we discussedTFIID(TBP) and TFIIH? Transcription factors mediate the binding of RNA polymerase and the initiation of transcription. Only after tx factors are attached to the promoter does RNA polymerase II bind to it. TFIID/TBP: TATA binding protein that recognizes the promoter sequence and recruits general transcription factors to bind and assemble TFIIH: A helicase enzyme that unwinds parent DNA and phosphorylates RNA polymerase II at the Cterminal domain. Phosphates become binding sites for "hitchhiker" proteins such as Capping Enzymes and Cleavage factors. Also used as a promoter clearance 24. In general terms, how do the functions of general transcription factors like TFIID and regulatory transcription factors differ? General tx factors are essential for transcription of all proteincoding genes. A few general tx factors bind to a DNA sequence such as the TATA box with in the promoter but most bind to proteins, including other tx factors and RNA pol II. Regulatory tx factors determine the rate of gene expression. Tx factors bind to activation domains that bind other regulatory proteins and tx machinery, facilitating a series of proteinprotein interactions that result in enhanced transcription off a given gene. Some tx factors act as repressors and can inhibit gene expression in several ways (directly binding to control element of DNA, blocking activator binding or interfering with the activator itself so it can't bind to the DNA) 25. What is a promoter? What is a TATA box and why is it called that? A promoter is the DNA sequence where RNA Pol II binds to begin transcription. The TATA box is a crucial sequence of DNA that is part of the promoter sequence where RNA polymerase binds. It is called this because the sequence is just that TATA. 26. What is the polyA signal sequence? What role does this sequence play in terminating transcription of a eukaryotic gene? What is its relationship to the polyA tail of eukaryotic mRNAs? The polyA signal sequence refers to the sequence TTATTT on DNA that is seen at the 5' end of the template strand that is transcribed in the 3'5' direction. When transcribed into mRNA it reads AAUAAA and is the sequence recognized by cleavage facts to end the transcription to create the premRNA strand. The polyA tail is added to the 3' end of the newly transcribed mRNA by polyA polymerase to increase efficiency of translation and to slow degradation 27. What is the 5' cap on eukaryotic mRNA? What is its functional significance? The 5' cap on mRNA is a methylated Guanine nucleotide that is added immediately after tx begins. The cap protects the mRNA from exonucleases and is the initiation of translation for the ribosome to bind. 28. What are exons and introns? What molecular machine is responsible for recognizing these sequences and controlling their processing in eukaryotic mRNAs? Exons are regions of mRNA that are expressed by being translated into AAs and Introns are regions of mRNA that are noncoding segments that are usually between coding regions and are cut out by large complex of proteins and snRNA called spliceosomes. Transcription factors that bind to a DNA sequence, other transcription factors, and RNA polymerase II facilitate the correct positioning of the DNAenzymetx factor complex on the promoter sequence of DNA and initiate transcription (RNA synthesis). The interactions of enhancers also affect the rate of gene expression by binding to activators or repressor proteins that in turn interact with the transcription initiation complex. It is the combinatorial code of all these things that regulate transcription. 29. What advantage does alternative splicing confer to eukaryotic cells? This is one of several opportunities for regulating gene expression. Different mRNA molecules are produced form the same primary transcript depending on which RNA segments are treated as exons and which as introns. Regulatory proteins specific to a cell type control intronexon choice by binding to regulatory sequences within the primary transcript. This expands the repertoire of a eukaryotic genome. The extent of alternative splicing greatly multiplies the number of possible human proteins, which may be better correlated with the complexity of the organism. 30. What is the relationship between the exon and a "functional domain" on a protein? How is this related to the concept of protein evolution over time? Different exons code for the different domains of a protein including the functional domains of the active sites and cell membrane binding sites. The presence of introns in a gene may facilitate the evolution of new and potentially beneficial proteins as a result of a process known as exon shuffling. Introns increase the probability of crossing over between the exons of alleles of a gene simply by providing more terrain for crossover without interrupting coding sequences. This might result in new combos of exons and proteins with altered structure and function. The occasional mixing and matching of exons between different genes could occur and this may also lead to new proteins with novel combinations of functions. 31. What are the four fundamental properties of the genetic code? Genetic code is written in triplets: 3 nucleotides in mRNA known as codons specify 1 amino acid. Codons are redundant so that each amino acid (except Met and Trp) encoded by more than 1 codon. Codons are unambiguous; each codon specifies only 1 amino acid "Universal" coding for all organism that use essentially the same codes for amino acids 32. What are the two "business ends" of a tRNA molecule? What is their functional significance in translation? The two business ends include the Amino acid attachment site which uses the AARS to attach an amino acid to the tRNA and the Anticodon end which has the complimentary nucleotides to the mRNA being translated. 33. How is it that the process of linking tRNAs to their cognate amino acids is a relatively high fidelity process, given the similarity in structure between some amino acids, for example leucine and isoleucine? The active site of each type of AARS fits only a specific combination of amino acid and tRNA. There are 20 different synthetases, one for each amino acid. Each synthetase is able to bind to all the different tRNAs that code for its particular amino acid. The synthetase catalyzes the covalent attachment of the amino acid to its tRNA in a process driven by the hydrolysis of ATP. 34. Describe the structure of the eukaryotic (80S) ribosome, what role is played by the large and small subunits of this molecular complex? Small subunit Binds to both mRNA and a specific initiator tRNA, which carriers the AA methionine. Binds the mRNA at a specific RNA sequence, just upstream from the start codon, AUG. It binds to the 5' cap of the mRNA and then moves, or scans, downstream along the mRNA until it reaches the start codon Large subunit Completes the initiation complex. Hydrolysis of GTP provides the energy for the assembly. Initiator tRNA is in the P site; the A site is available to the tRNA with the next AA 35. What do the "E", "P" and "A" stand for in describing the three tRNA binding sites within the large ribosomal subunit? Describe the cycling of tRNAs through these three sites during the process of translation. What is happening to the mRNA molecule as this is occurring? The P site (peptidyltRNA binding site) holds the tRNA carrying the growing polypeptide chain while the A site (aminoacyltRNA binding site) holds the tRNA carrying the next amino acid to be added onto the chain. Discharged tRNAs leave the ribosome from the E site (exit site). The positions of the ribosome hold the tRNA and mRNA in a close proximity and positions the new amino acid so that it can be added to the carboxyl end of the growing polypeptide. It then catalyzes the formation of the peptide bond. 36. What is the general role of initiation factors in translation? What roles are played by elongation factors? What role is played by release factors? Initiation factors are required to bring all the components of translation together. The cell also expends energy obtained by hydrolysis of a GTP molecule to form the initiation complex. Elongation factors are required by the addition of amino acids to the growing polypeptide chain. The mRNA is moved through the ribosome in one direction (same as saying ribosome moves 5'3' down RNA). Codon recognition requires hydrolysis of one molecule of GTP, which increases the accuracy and efficiency. One more GTP is hydrolyzed to provide energy for the translocation step. Release factors bind directly to the stop codon in the A site. The release factor causes the addition of a water molecule instead of an amino acid to the polypeptide chain. This reaction breaks the bond between the completed polypeptide and the tRNA in the P site releasing the chain through the exit tunnel of the ribosomes large subunit. 37. How does a given mRNA direct protein synthesis by either free ribosomes in the cytoplasm, or bound ribosomes at the rough ER? Polypeptide synthesis begins on a free ribosome in the cytosol. A signalrecognition particle binds to the signal peptide which targets the protein to the ER. The SRP binds to a receptor protein complex that forms a pore and has a signalcleaving enzyme. The SRP leaves and polypeptide synthesis resumes, with simultaneous translocation across the membrane. The signal cleaving enzyme cuts off the signal peptide. The rest of the completed polypeptide leaves the ribosome and folds into tits final conformation. 38. What is meant by the terms silent, missense, nonsense and frameshift mutation? How can a base change in a protein coding gene that causes a single amino acid substitution have either no effect, minimal effect or devastating effect on the protein function? Point mutations are changes in a single nucleotide causing the following mutations: o Silent mutation is a change in a nucleotide pair that may transform one codon into another that is translated into the same amino acid and there is no observable effect on the phenotype. o Missense mutations are substitutions that change one amino acid to another. This mutation may have little effect on the protein because the new amino acid has similar properties as the one it replaced or it may be in a region of the protein where the exact sequence of amino acids is not essential to the proteins function. This may be detrimental if the new amino acid is substantially different than the one it replaced or if the region in the protein complex is sensitive to changes. o Nonsense mutations occur when a point mutation to a nucleotide changes an amino acid to a stop codon and it causes translation to be terminated prematurely; the resulting polypeptide will be shorter than the polypeptide encoded by the normal gene. These usually lead to nonfunctional proteins. Insertion and deletions are additions or losses of nucleotide pairs in a gene. These have devastating effects on the resulting proteins and the organism as a whole. o Frameshift mutations are the alterations of the reading frame of the genetic message, the triplet grouping of nucleotides on the mRNA that is read during translation. All nucleotides downstream of the deletion or insertion will be improperly grouped into codons and will result in extensive missense usually ending sooner or later in nonsense and premature termination. 39. How could a mutation within an intron lead to disruption of protein function? What percentage of human diseases are thought to be the result of this type of mutation? A mutation in an intron may disrupt protein function because a mutation in an intron would cause a different site for RNA splicing. This leads to different outcomes of exons which ultimately serve as the coding regions of mRNA. 40. What is the molecular explanation for the diauxic growth curve of a culture of E. coli grown in the presence of glucose and an alternate sugar like lactose? The biphasic bacterial growth in the presence of glucose and alternate sugars like lactose is due to the preferred sugar by E. coli. A shift then occurs in the expression in genes to metabolize the next sugar. Bacteria utilize the glucose first because more energy from the monosaccharide than having to isomerize and split the disaccharide lactose. 41. What are the functions of the three structural genes of the lac operon of E. coli? Which, if any, of the three genes are indispensable for normal lactose metabolism? Lac Z: encodes the lactose betagalactosidase which cleaves and begins metabolism of lactose. Lac Y: encodes for lactose permease which is a membrane transport protein Lac A: (dispensible) encodes galactosidase transacetylase enzyme which is not understood completely 42. Distinguish between the promoter, operator and CAP binding sites of the lac operon. Where are they located relative to the structural genes and to one another? Which of these are considered "regulatory elements"? What are they regulated by? Why is the I gene expressed independently of the lac operon structural genes? Promoter directs RNA polymerase to bind to gene and begin transcription located upstream next to operator and upstream from the +1 location. Operator is the binding site for the regulatory protein that negatively regulates transcription known as a repressor. It is located next to +1 and the lac Z gene. CAP Site is where the regulatory protein CAP binds which is located next to and upstream from the promoter. CAP protein interacts with RNA polymerase II to stabilize promoter because the promoter is "weak" without it. The CAP site is considered regulatory because when cAMP binds to the CAP protein it is because glucose is not present. This occurs because glucose will block adenylyl cyclase from catalyzing cAMP. The I gene is expressed independently because it encodes for a repressor regulatory protein. This repressor will bind to the operator site when no lactose is present because there is no allolactose to allosterically inhibit the repressor from binding. 43. Why is the lactose operon referred to as an inducible operon? What is the molecular mechanism of induction? How does this mechanism effectively allow the E. coli genome to "sense" when lactose is present? The lac operon is referred to as inducible because it is usually off but can be stimulated when a specific small molecule interacts with a regulatory protein. The regulatory protein is the, usually inactive, repressor protein that gets activated in the presence of allolactose (lactose). 44. What is the molecular mechanism that leads to higher levels of lac operon transcription when glucose is absent? How does this mechanism effectively allow the E. coli genome to "sense" when glucose is present? When glucose is not present cAMP concentrations can increase because its catalysis is not inhibited by the presence of glucose. The cAMP allosterically activates CAP which can bind to the CAP site on the operon. When CAP binds it increases the transcription rate of RNA polymerase II. It acts as a sensor for the presence of glucose because if glucose where present, concentrations of cAMP would be lower and CAP would be less likely to be activated. 45. Generally speaking, what "types" of eukaryotic genes are regulated and what types would you expect to be expressed constitutively over the life of the cell? Why? Constitutive genes are not regulated and may code for proteins that are necessary for normal cell function such as cell membrane proteins, organelles, etc. Facultative genes are regulated as needed such as genes signaling rapid cell replication as seen during embryonic development. 46. What functional distinction can be made between general transcription factors like TFIID and gene specific transcriptional activators and repressors? General transcription factors are used by both eukaryotes and prokaryotes; regulatory transcription factors are used by eukaryotes. General tx factors like TFIID are required for transcription
Buy Material
Are you sure you want to buy this material for
50 Karma
BOOM! Enjoy Your Free Notes!
We've added these Notes to your profile, click here to view them now.
You're already Subscribed!
Looks like you've already subscribed to StudySoup, you won't need to purchase another subscription to get this material. To access this material simply click 'View Full Document'
Why people love StudySoup
"There's no way I would have passed my Organic Chemistry class this semester without the notes and study guides I got from StudySoup."
"Selling my MCAT study guides and notes has been a great source of side revenue while I'm in school. Some months I'm making over $500! Plus, it makes me happy knowing that I'm helping future med students with their MCAT."
"Knowing I can count on the Elite Notetaker in my class allows me to focus on what the professor is saying instead of just scribbling notes the whole time and falling behind."
"It's a great way for students to improve their educational experience and it seemed like a product that everybody wants, so all the people participating are winning."
Refund Policy
All subscriptions to StudySoup are paid in full at the time of subscribing. To change your credit card information or to cancel your subscription, go to "Edit Settings". All credit card information will be available there. If you should decide to cancel your subscription, it will continue to be valid until the next payment period, as all payments for the current period were made in advance. For special circumstances, please email firstname.lastname@example.com
StudySoup has more than 1 million course-specific study resources to help students study smarter. If you're having trouble finding what you're looking for, our customer support team can help you find what you need! Feel free to contact them here: firstname.lastname@example.com
Recurring Subscriptions: If you have canceled your recurring subscription on the day of renewal and have not downloaded any documents, you may request a refund by submitting an email to email@example.com
Satisfaction Guarantee: If you're not satisfied with your subscription, you can contact us for further help. Contact must be made within 3 business days of your subscription purchase and your refund request will be subject for review.
Please Note: Refunds can never be provided more than 30 days after the initial purchase date regardless of your activity on the site.


text:
Preview & discussion of the film "We Still Live Here As Nutayunean."
The Wampanoag saved the Pilgrims from starvation, and lived to regret it. Spurred on by their celebrated linguist Jane Little Doe Baird, the Wampanoag of Cape Cod and Martha's Vineyard are reviving their language more than a century after the last native speaker died.
Visit the Rutland Free Library website for more information.
Community Cinema is presented locally as a partnership between VPT, Brattleboro Museum and Art Center, Brattleboro Community TV, Catamount Arts, the Flynn Center for the Performing Arts, Osher Lifelong Learning Institute (OLLI) at UVM and the Rutland Free Library.
Vermont PBS educates, informs, entertains and inspires Vermonters to be lifelong learners and engaged in their community.
Recorded live at the 2016 Burlington Discover Jazz Festival, we're back with Season 2 of Discover Jazz
Family-friendly Halloween party for kids grade 5 and under!
Join our Vermont PBS Kids' Club!
PBS Kids - many videos and games for children!
Educator Resources - for teachers and parents.
PBS Parents - your child's first, best teacher.
Learn about our second HD broadcast service offering alternative programming and more content from local and regional producers.
Our weekly email newsletter highlighting upcoming shows and important station news. Sign up today!


text:
Hotels in Anse Marcel, St. Martin
Search & Compare Anse Marcel Hotels
Get Secret Prices on select hotels
These prices aren't available to everyone.
Trending now in Anse Marcel
Hotels in Anse Marcel
- Most bookedRiu Palace St Martin All Inclusive
78 people looked at this hotel in the last hour
Most recent review
"Very pleasant stay. Very professional and accommodating staff"
A Traveller, Oct 2016, US
49 people looked at this hotel in the last hour
Most recent review
"The Marquis is in a beautiful location on Saint Martin and is the perfect escape for vacationers looking for a nice place to stay. Located on a hill overlooking the entire cove, the fantastic view and cool breeze are the perfect thing to relax to after a long day on the beach. The hotel is walkin..."
A Traveller, Jun 2016, US
30 people looked at this hotel in the last hour
Most recent review
"My wife and I loved this place. Its located on one of the best beaches in St Martin. However you do need to be prepared for a challenging drive over the mountain/hill to get there, its very steep & a lot of curves but I think the drive just adds adventure. The view from the beach to the ocean is..."
A Traveller, Apr 2016, US
24 people looked at this hotel in the last hour
Most recent review
"Our 8 nights stay at Privilege couldn't be more perfect! Privilege is on top of the hill and we loved the view you get of Anse Marcel right when you wake up! We enjoyed our quaint breakfast - basket of croissant and bread, French ham and Swiss cheese, honey & marmalade, very good coffee or tea or..."
O, Mar 2016, US
9 people looked at this hotel in the last hour
Hotels within 5 miles of Anse Marcel
123 people looked at this hotel in the last hour
Most recent review
"Small, intimate beach club. Rooms are updated with great water views. Beach is lovely as is new pool. Friendly staff. Went during hurricane season so not crowded and weather was perfect during our stay. There is no night life if you are looking for that. Restaurants in town are superb. You can wa..."
A Traveller, Sep 2016, US
58 people looked at this hotel in the last hour
Most recent review
"Free gifts. Friends the drinking. A++++++++++++++++++++++ Parking Friendly staff"
A Traveller, Oct 2016, US
34 people looked at this hotel in the last hour
Most recent review
"The location was wonderful and the staff were helpful and very nice. The hotel is getting rundown, but that was also reflected in a slightly cheaper price. "
A Traveller, Sep 2016, US
31 people looked at this hotel in the last hour
Most recent review
"The hotel is just a short walk from the beach. Its nicely maintained with a friendly staff and clean comfortable rooms."
A Traveller, Apr 2016, US
18 people looked at this hotel in the last hour
Most recent review
"Could not have asked for a better experience. Staff was very helpful. Pastries served fresh and warm every morning. The pictures that you see on the hotels' site are exactly what you see...nothing but beautiful turquoise waters. The locals are exceptionally friendly. RENT A CAR, the island has be..."
B, Sep 2016, US
Best hotels in Anse Marcel
You can compare all of our Anse Marcel hotel rooms including the major Anse Marcel hotel chains using our genuine Hotels.com guest reviews as well as TripAdvisor Anse Marcel hotel reviews to help find the perfect hotel room when booking your accommodation in Anse Marcel. We also feature Expedia Anse Marcel hotels and reviews.
When you book your Anse Marcel stay with Hotels.com you may also earn free nights on participating hotels by joining the Hotels.com Welcome Rewards program. It's free to join and only takes 2 minutes to sign up and when you stay 10 nights you receive 1 night free*. So even a short weekend break in Anse Marcel can get you on your way to a free night.
Why use Hotels.com to book your Anse Marcel lodging & accommodation?
We can help you to find the perfect Anse Marcel hotel room for almost any occaision. With our fast and secure hotel search you may compare different Anse Marcel hotels by:
- - Price
- - Location within Anse Marcel
- - Neighborhoods in Anse Marcel
- - Nearby Anse Marcel attractions
- - Hotels in different areas of St. Martin
- - Genuine Hotels.com guest reviews for Anse Marcel
- - Star rating
- - Amenities
- - Hotel name & chain
- - Theme
For every hotel in Anse Marcel St. Martin we provide:
- - Up to the minute hotel rates & availability
- - In depth hotel information and photos
- - Detailed Anse Marcel maps showing the locations of your hotel in Anse Marcel and the nearest transport and landmarks
- - TripAdvisor Anse Marcel hotel review score
And when you make a hotel reservation in Anse Marcel with Hotels.com we'll send you email and text confirmations with the reservation details of your Anse Marcel hotel booking along with contact details, directions, information on nearby Anse Marcel attractions, restaurants and even the weather - and if you get stuck, we're only a phone call away.
To make the most of our service, download our mobile & tablet apps and sign up to our newsletter or follow us on Facebook, Google+ or Twitter to receive the latest deals and Hotels.com Anse Marcel discount codes.