text:
Author Archive
Martha Chishala Maintains Order at AGCO's Future Farm in Zambia
Earlier this month, we introduced you to Richard Chapple, Farm Manager at the AGCO Future Farm in Zambia. Today, we'd like to give you another glimpse into daily life at the farm by introducing Martha Chishala, Training Centre Coordinator.
Martha plays an integral part in keeping things running smoothly at the Zambia Future Farm. Her role includes organizing training events and ensuring everything related to training is in order—from updating trainer schedules, to ordering supplies, to coordinating work visas.
She was on hand at the official launch of the Future Farm on May 27. "The official opening event was exciting, as there were so many great presentations and demonstrations of what AGCO plans to do in Africa and change the way of farming and agriculture," she says. "It encouraged us all to embrace mechanised farming."
Martha joined the AGCO Future Farm team in May of 2015, but she has already seen the impact it has had on the community. "The farm has created jobs for the local community and supports projects such as Chikumbuso Women and Orphans project, and it teaches children at a young age about the values of agriculture," she says.
The farm faces many challenges, such as the area's low water table, which can only sustain one winter crop per year. But Martha finds it rewarding to be part of this new age of farming, and she is learning that there is more to agriculture than just putting seed in the ground.
"I see a generation of farmers and changed mindset in regards to agriculture," she says. "Growing up in Zambia we believed farming was for the uneducated, rural based-based older folk—something people do after retirement. I never considered a career prospect, but with empowerment through knowledge, all that is changing."
Jeff Challis Shows You Why You Should Buy Genuine AGCO Parts Filters
A visit to Kenya prompts the production of a video to demonstrate the merits of buying genuine AGCO filters and how to spot counterfeit parts.
In March 2014 Jeff Challis, Business Manager, Parts Sales, Africa and Middle East and Pete Winterbottom, Manager, Aftersales Marketing, EAME visited Kenya to meet with our Massey Ferguson distributor FMD and visit some of their key customers. These customers varied from important municipal customers such as Nairobi Airport to owners of large tea and sugar plantations. The purpose of the visit was to discuss the virtues of genuine parts. Jeff demonstrated this by dismantling a genuine filter and comparing key features with a popular spurious filter. From the outside both filters looked the same but Jeff quickly highlighted that underneath this veneer there were some significant differences. Jeff's demo had such a positive impact that FMD said they wanted to train and equip their own staff to do the same demo when on the road visiting customers. To assist with this, the EAME Aftersales Marketing team produced some practical demo kits including genuine and spurious filters along with flip chart slides. Jeff took the kits back to Kenya and also Sudan in early 2015 to train the local field sales teams to use themselves.
As an aide memoire for the local teams, we filmed Jeff at the Stoneleigh training centre conducting the demo. A group of UK service technicians that were on a training course in an adjacent workshop at time of filming stopped what they were doing because they too were so intrigued to see and hear what Jeff had to say. So much so that we decided to publish the resultant videos on YouTube for all to see.
We've created 2 versions of the demo; a 3 minute and 15 minute version which can be watched on YouTube or downloaded from asset bank. We're now considering the possibility of creating different language versions in order to spread this key message: – When it comes to Parts 'The Genuine Choice' is the only choice!'
To learn more about AGCO Parts please visit: http://www.agcocorp.com/parts-service.html.
Meet Richard Chapple, Farm Manager at AGCO's Future Farm in Zambia
In recent posts, we shared our vision for the AGCO Future Farm concept, and in May we celebrated the official opening of our first Future Farm in Lusaka, Zambia. Today, we'd like to introduce one of the team members making this project successful: Farm Manager Richard Chapple.
Originally from the UK, Richard came to Zambia in November 2008 to visit family, but he was offered a job running a flight charter company and stayed. With a background as an agricultural contractor in the UK and experience sub-contracting combines in Zambia on behalf of a company called African Harvesters, he was a natural fit for AGCO and joined the Future Farm team in 2012.
Although every day on the farm is different, a typical morning for Richard starts at 7:30 a.m., when he has a meeting with his team of 32 workers. They allocate jobs based on what is planned for the day, from spraying programs to planting a variety of crops around the farm.
The Zambia Future Farm includes a state-of-the art facility designed to accommodate both small-scale and large commercial farmers, as well as education and training programs to provide hands-on experience with technology and utilize Africa's agricultural resources more effectively. Chapple says this is reassuring to local farmers. "No matter what tractor you're driving, it's all about the support you're receiving."
Chapple has been involved with the Future Farm project since its inception in 2012, and he said the team experienced a great sense of achievement at the official launch on May 27. "In a small space of time, we've done a huge amount of work," he said. "It's like a jigsaw puzzle and all the pieces came together."
What does Chapple find most rewarding about his job? "For me, it's development, and not just of the farm itself," he said. "When we took over the farm, we also took over the workforce that was here already. It's the personal development of the workforce on the farm, the capacity building, and getting better relationships. I've learned a fair bit, as well."
Zambia has huge potential in terms of resources to be tapped, and Chapple appreciates the opportunity to play a role the development of agriculture in the country. "I'm very excited and happy to be a part of it."
Cultivating Farms of the Future
We have a vision to change the future of farming. In 2013, we planted the seed for a Future Farm and training center to empower the world's farmers with education, training and technology. This month, the first AGCO Future Farm officially opens in Zambia. The state-of-the-art facility includes a mechanized solutions center, a grain and poultry learning center, and training programs focused on products, machines, business management and farming best practices.
Why Zambia?
AGCO has a strong commitment to agriculture in Africa. The Republic of Zambia is landlocked, bordered by eight neighboring countries. Its central location coupled with an economic focus on farming (agriculture accounts for 21.5 percent of the nation's GDP) makes Zambia an ideal place for AGCO's Future Farm prototype.
The Future Farm concept will develop a sustainable food production system that is able to increase farm output by utilizing Africa's agricultural resources more efficiently. The Zambia center will facilitate agricultural support services to enable knowledge and increase food production on other area farms.
And Future Farm Zambia is more than just a training facility—it's also a working farm. In 2015, 70.22 hectares were planted with maize, soybeans, sunflowers, sun hemp and other crops. The farm's expected yields this year include 7.5 tonnes/hectare of maize and 1.4 tonnes/hectare of soybeans.
Better Together
In addition to area farmers, local organizations will benefit from the Future Farm as well. AGCO Zambia has supported the Chikumbuso Women and Orphans Project in Lusaka, which provides free education for children, adult training and community building programs. The Future Farm donates soybeans and maize to help the project's feeding program and kitchen enterprise.
In addition, AGCO Zambia supports the Chalimbana Youth project in partnership with the Africa 4H Council and Future Farm Partners. As part of this project, younger children learn agricultural practices and grow food for their families through an Enterprise Garden, and older participants learn to start and sustain small agro-enterprises through an entrepreneurship group.
The AGCO Future Farm project has the potential to have a significant impact through a range of agricultural solutions, such as crop protection, precision farming, seeding and fertilizing, and strong partnerships will be key to its success. Additional Future Farms partners include AquaCheck, Bayer Crop Science, Harper Adams University, Musika, GCS Water Management, Seedco, University of Zambia, Valley and Yara.
"I believe we have a unique opportunity to develop a new vision for how small, medium, and large-scale farmers can develop not just in Zambia, but throughout the region," said Mark Briggs, director of Precision Decisions Zambia Limited and also Co-Chair for the Future Farm Partners Steering Committee. "The idea of having a group of experts, not just from the manufacturing sector but also service sectors, engaged in developing products and services, offering training to farm workers, managers and, indeed, individuals working in the broader agricultural arena, is very exciting."
What does the future hold for the Future Farm? Stay tuned to our blog or follow @AGCOcorp on Twitter for updates from Zambia and other AGCO initiatives around the world.
AGCO realiza aulas inaugurais do Pescar 2015
As unidades de Canoas, Santa Rosa, Ibirubá e Mogi das Cruzes acolheram as novas turmas do Projeto Pescar 2015 e seus familiares para a aula inaugural. As duas primeiras unidades realizaram o evento em 2/3 e as duas seguintes no dia 9/3. Durante o evento, eles conheceram os voluntários, receberam as boas vindas das lideranças e realizaram uma visita guiada pela área fabril.
Durante o ano, eles participarão de aulas sobre cidadania, ética e solidariedade, bem como desenvolver competências importantes para o crescimento profissional, preparando-os também para contribuir com a sociedade de forma integral. Tudo isso com a participação comprometida dos voluntários.
A AGCO já formou 424 jovens, ao longos dos últimos 11 anos do projeto e se propõe a oportunizar o acesso à iniciação profissional de jovens em situação de vulnerabilidade social.
AGCO performs inaugural class of Pescar 2015
The unit of Canoas, Santa Rosa, Ibirubá and Mogi das Cruzes welcomed the new group of Projeto Pescar 2015 and their families for the inaugural class. The first two units performed the event on March 2nd and the two following on March 9th. In the event, they met the volunteers, were welcomed by the leaders and held a guided tour of the factory area.
During the year, they will participate in lessons of citizenship, ethics and solidarity and to develop important skills for professional growth, preparing them also to contribute to society in full. All of this with the committed participation of volunteers employees.
AGCO has graduated 424 young people over the last 11 years of the project that has the aims to provide the opportunity for access to professional initiation of young people in social vulnerability.


text:
|Home | About | Journals | Submit | Contact Us | Français
Conceived and designed the experiments: HG LZ KH. Performed the experiments: HG XZ BC YS CL TL. Analyzed the data: HG LZ KH. Wrote the paper: HG LZ KH.
Bisphenol A (BPA) is a chemical compound widely used in manufacturing plastic products. Recent epidemiological studies suggest BPA exposure is positively associated with the incidence of type 2 diabetes mellitus (T2DM), however the mechanisms underlying this link remain unclear. Human islet amyloid polypeptide (hIAPP) is a hormone synthesized and secreted by the pancreatic β-cells. Misfolding of hIAPP into toxic oligomers and mature fibrils can disrupt cell membrane and lead to β-cell death, which is regarded as one of the causative factors of T2DM. To test whether there are any connections between BPA exposure and hIAPP misfolding, we investigated the effects of BPA on hIAPP aggregation using thioflavin-T based fluorescence, transmission electronic microscopy, circular dichroism, dynamic light scattering, size-exclusion chromatography,fluorescence-dye leakage assay in an artificial micelle system and the generation of reactive oxygen species in INS-1 cells. We demonstrated that BPA not only dose-dependently promotes the aggregation of hIAPP and enhances the membrane disruption effects of hIAPP, but also promotes the extent of hIAPP aggregation related oxidative stress. Taken together, our results suggest that BPA exposure increased T2DM risk may involve the exacerbated toxic aggregation of hIAPP.
Diabetes is a panepidemic endocrine disease, with approximately 285 million diagnosed patients worldwide [1]. Non insulin dependent diabetes or type 2 diabetes mellitus (T2DM) accounts for more than 90% of diagnosed diabetes [2]. An important causative factor of T2DM is the misfolding of human islet amyloid polypeptide (hIAPP), which is a 37-residue peptide synthesized and secreted by the pancreatic β-cells (Fig. 1A; [3]). Despite the important physiological functions including glycemic control and regulation of certain hormones [4], hIAPP has a high intrinsic propensity to misfold into toxic oligomers and linear fibrils [5]. During this transition, natively unstructured hIAPP monomers first form β-structure rich oligomers, which further assemble into mature linear fibrils through lateral growth and elongation [6]. The cytotoxicity of hIAPP is generally attributed to the membrane permeabilization ability of hIAPP oligomers and mature fibrils, which cause apoptosis and eventually the onset of diabetes [7]–[10]. Therefore, preventing the formation of toxic hIAPP amyloid has been viewed as a plausible therapeutic approach for T2DM [11].
Bisphenol A (BPA; Fig. 1B) is a compound widely used in polycarbonate, epoxy resins and other polymer materials for manufacturing plastic utensils. The leach of BPA from plastic products is considered an important environmental issue [12]. Humans are exposed to BPA primarily through oral and inhalation routes [13]. BPA exposure is associated with multiple diseases, such as diseases of the reproductive system, nervous system and sexual dysfunction [14]–[16], as well as increased risk of cancer and heart disease [17], [18]. Although the exact molecular mechanisms of BPA toxicity remain unclear, official policies have been enacted or are being considered in many countries to reduce the BPA exposure worldwide [19].
Recent epidemiological evidence suggests that a concentration dependent correlation exists between BPA exposure and the occurrence of diabetes. BPA levels have been found significantly higher in both diagnosed diabetic and borderline diabetic patients than those of non-diabetic subjects [20]. A strong association between high urinary levels of BPA and diabetes has been identified by studying 3400 residents in China that a 37% increase in the incidence of T2DM being observed in subjects with urinary BPA concentration above 1.43 ng/ml compared with the reference concentration (≤0.47 ng/ml) [21]. In a clinic investigation with 1455 adults, the risk of diabetes in the highest BPA concentration group was 2.43 times higher compared with those in the lowest concentration group [22]. Additionally, severe metabolic disorders of glucose homeostasis and insulin resistance, hallmarkers of T2DM that are directly correlated with impaired pancreatic β-cell function, have been also observed in normal mice exposed to BPA [23], [24].
It is well recognized that environmental factors, including multiple metal ions, polyphenols, fatty acids and certain natural products of small molecule size, can affect the toxic misfolding of hIAPP and may cause diabetes [25]–[29]. We thus hypothesize that BPA exposure may associate with diabetes through promoting the toxic aggregation of hIAPP. To test this hypothesis, the effects of BPA on hIAPP aggregation were investigated in this work.
Synthetic hIAPP (1–37) was obtained from Genscript Inc. (Piscataway, NJ, USA). Bisphenol A was obtained from Aladdin-reagent (Shanghai, China). Carboxyfluorescein, thioflavin-T (ThT), 2-Oleoyl-1-palmitoyl-sn-glycerol-3-phospho-rac (1-glycerol) sodium salt (POPG) and hexafluoroisopropanol (HFIP) were purchased from Sigma-Aldrich (St. Louis, USA). INS-1 cells were obtained from the China Center for Type Culture Collection (CCTCC). All other chemicals were of the highest grade available.
For all experiments, hIAPP was freshly dissolved in HFIP and vigorously sonicated for 2 min to homogenize the sample. After a short-spin, the solution was diluted to desired concentration in 25 mM sodium phosphate buffer (pH 7.4) containing 50 mM NaCl, and a final HFIP concentration of 1%. Freshly prepared BPA stock solution was then immediately added to desired concentrations,thoroughly mixed and ready for further analysis. The whole preparation process is strictly limited to 5 min.
CD spectra were obtained with a JASCO-810 spectropolarimeter at 25°C under a constant flow of N2. Freshly dissolved hIAPP was diluted to a final concentration of 15 µM,. Spectra were obtained from 260 to 190 nm with a 2 nm bandwidth, 1 s response time, 50 nm/min scanning speed and a 1 mm pathlength. Each sample was measured at least three times and the spectra were averaged to give the final result. Spectra of PBS buffer containing corresponding concentrations of BPA were measured as the baselines. The final spectra were obtained by subtracting corresponding baseline spectrum from sample spectrum, which were further converted to mean residue ellipticity [θ] and were analyzed with the software CDPro using the CONTINLL algorithm as previously described [30].
Freshly prepared hIAPP solution (15 µM) was incubated at 25°C for amyloid formation in the presence of different molar ratios of BPA. ThT fluorescence assays were preformed on a Hitachi FL-2700 fluorometer to detect the formation of amyloid at designated time points. The final assay solution contains 25 mM PBS (pH 7.4), 50 mM NaCl and 20 µM thioflavin-T [27]. ThT fluorescence was recorded at 482 nm with an excitation wavelength of 450 nm. PBS buffer containing different concentrations of BPA were measured as the controls. All of the experiments were performed at least three times, and the lag times were calculated as we previously described [31].
The TEM was performed as previously described [32]. Briefly, 5 µl of sample was applied onto a 300-mesh Formvar-carbon coated copper grid. Excess solvent was removed carefully and stained by dropwise addition of 1% freshly prepared uranyl formate followed by air drying. Images were observed under a transmission microscope (Hitachi, Tokyo, Japan) operating at an accelerating voltage of 100 kV.
POPG was dissolved in chloroform at a concentration of 10 mg/mL. Chloroform was then removed under a stream of N2, and samples were dried under vacuum to remove residual chloroform. Multilamellar vesicles were made by mixing dry POPG films with 25 mM PBS (pH 7.4) containing 40 mM carboxyfluorescein. PD-10 columns (Sangon, Shanghai, China) were then used to remove nonencapsulated carboxyfluorescein as previously described [33]. POPG vesicles containing carboxyfluorescein were diluted in 25 mM PBS (pH 7.4) for florescence measurements. hIAPP stock solution was added to POPG vesicles at a final concentration of 1 µM immediately before measurement. The samples were excited at a wavelength of 493 nm, and the emission was detected at 518 nm. The fluorescence signal was recorded for 90 s, POPG vesicles alone were tested as the baseline and the signals of POPG vesicles treated with 0.2% (v/v) Triton X-100 (for complete membrane leakage) were used as the positive control. All measurements were repeated at least three times.
The SEC analysis was performed on a Tosoh TSK GW2000 column (Tokyo, Japan). hIAPP was freshly prepared to a final concentration of 30 µM,mixed with different amounts of BPA, and were immediately injected into a Hitachi L-2000 HPLC system, and the column was eluted with a 20% acetonitrile containing 0.003% TFA at a flow rate of 0.3 ml/min as previously described [34].
Dynamic light scattering was performed by using a zeta pals potential analyzer (Brookhaven Instruments, New York, USA). 30 µM hIAPP was measured in a 200 µl cuvette incubated at 37°C with a scattering angle of 90°. The starting time for the very first sample scan was marked as time zero. All of the samples were scanned for three times (4 min/scan) and the mean particle size was recorded and analyzed by the multimodal size distribution (MSD) software.
Pancreatic INS-1 cells were cultured in 1640 medium containing 10% FBS, 1% sodiumpyruvat, 1% penicillin-streptomycin solution and 50 µM β-mecaptoethanol. And cells were plated in 96-well plates at a density of 5×104 cells/well and incubated at 37°C in 5% CO2 atmosphere for 24 h. The medium was then replaced with fresh medium containing hIAPP (5 µM) and varied amounts of BPA for 24 h further incubation. Cells treated with BPA or PBS were used as the controls. For MTT assay, cells were co-incubated with 10 µl MTT (5 mg/ml) per well for 4 h. 100 µl formazan buffer was then added to each well and the absorbance was measured at 570 nm [35]. Synergistic effects were analyzed by calculating coefficient of drug interaction (CDI) [36].
INS-1 cells were seeded into 6-well plates and treated with or without 10 µM hIAPP and different ratios of BPA for 12 h. The harvested cells were washed by PBS and incubated in 1640 medium containing 10 µM carboxy-H2DCFDA (Beyotime, Shanghai, China) for 20 min at 37°C. The cells were then washed twice with PBS and the levels of ROS were detected by a flow cytometer (Beckman, USA) with an excitation wavelength of 485 nm and an emission wavelength of 530 nm [37].
The Kruskal-Wallis test and the Mann-Whitney test were used to evaluate statistical significance. All results were expressed as the mean ± SD. Difference was considered statistically significant at P<0.05.
The transition from random coil structure to predominant β-structure is characteristic of hIAPP amyloid formation [26]. To investigate the effects of BPA on the secondary structure transition of hIAPP during incubation, far-UV CD spectroscopy was applied. At the beginning of incubation, the CD spectrum of hIAPP was characteristic of predominant random coil structure (Fig. 2A), which agreed with previous reports [38], [39]. An intensity increase of a negative band between 210~220 nm was observed after 6 h of incubation, which represents a conversion from random coil to β-sheet-rich structures (Fig. 2A). After incubating for 12 h, a negative peak near 220 nm was observed, which is a typical sign of β-structures (Fig. 2A). The molar ratio of BPA to hIAPP (11 and 15) tested were determined according to a pilot fluorescence study (data not shown). BPA at molar ratios of 11 and 15 showed no immediate effects on the secondary structure of hIAPP, however, the rate of structural transition was much faster than that of hIAPP alone (Fig. 2B & 2C). In the presence of equimolar amounts of BPA, a notable structural change to the β-sheet conformation was observed at 2 h, which was 4 h earlier than that of hIAPP alone (Fig. 2B). When the molar ratio of BPA was further increased to 5-fold, the broad negative peak at 2 h was similar to the spectrum of hIAPP alone recorded at 12 h with the structural change completion time accelerated to less than 2 h (Fig. 2C). These data suggested BPA dose-dependently promotes the secondary structural transition of hIAPP to the β-sheet-rich structures.
Further CDPro deconvolution analysis confirmed the formation of β-structures (Fig. S2). CD data of hIAPP with or without BPA all showed a significant reduction in random coil structure with an accompanied increase of β-structures (β-sheet and β-turn) after the 24 h incubation (Table S1). For all sample groups, the final percentages of β-structures were greater than 46%. However, the percentage of β-structures at 2 h increased to 35.5% and 43.1% in the presence of 1-fold and 5-fold BPA, respectively, which was significantly higher than that of hIAPP alone (26.5%).
The existence of β-sheet-rich structure is characteristic of amyloid fibrils [40]. Thioflavin-T (ThT) is a fluorescence dye that binds specifically to β-sheet structures and is widely used in probing the emerging β-structures during amyloid formation [41]. hIAPP with different molar ratios of BPA were incubated at 25°C for 24 h. ThT fluorescence was checked every 2 h to monitor the kinetics of fibril formation. hIAPP alone gave a strong ThT emission with a lag time of 5.87±0.77 h (Fig. 3A). The addition of BPA not only dose-dependently shortened the lag time, but also significantly increased the intensity of the maximum fluorescence (Fig. 3B & Table S2). The addition of 0.5-fold BPA shortened the lag time from 5.87±0.77 h to 4.05±0.09 h (P<0.05); when the ratio of BPA/hIAPP was further increased to 1, 2 and 5, the rates of hIAPP misfolding were notably accelerated, with the lag time reduced to 2.98±0.08 h (P<0.05), 2.52±0.23 h (P<0.05) and 1.92±0.19 h (P<0.05), respectively (Table S2). In the presence of 10-fold BPA, the lag time was even reduced to 0.96±0.23 h (P<0.05). The maximum fluorescence intensity also increased notably with the increasing ratios of BPA, with the greatest increment being more than 3 times that of hIAPP alone (Fig. 3B). Control fluorescence spectra recorded with BPA alone indicated BPA by itself does not contribute to this fluorescence intensity increment (Fig. S1).
Morphology of hIAPP aggregates was observed by TEM. After incubating for 2 h, TEM images of hIAPP with or without 0.5-fold BPA showed no sign of aggregation; scattered short fibrils or protofibrils were found only after 4 h incubation (Fig. 4). Classic long linear hIAPP amyloid fibrils were identified after incubating for 8 h (data not shown). In contrast, typical fibrils appeared much earlier in BPA treated groups (Fig. 4), for example, in the 10-fold BPA group, extensive long linear amyloid fibrils were observed at 2 h, which further grew and formed a fibril mesh at 4 h (Fig. 4). Control TEM images of BPA alone was also obtained and no obvious particle background was found (Fig. S1). These observations are consistent with the results obtained from the ThT fluorescence and CD assays.
The oligomerization status of hIAPP was analyzed using size-exclusion chromatography (SEC). Freshly dissolved hIAPP showed a monomer peak eluting at 25 min with a calculated MW of 3.9 kD (Fig. 5). The sample with 5-fold BPA showed a leading peak at 24 min (calculated MW of 7.9 kD), suggesting the formation of an hIAPP dimer. In the presence of 10-fold BPA, the dimer peak almost disappeared with a new peak eluting at 22.5 min (calculated MW of 12 kD), indicating the formation of a trimer. These data suggested BPA dose-dependently accelerates the oligomerization of hIAPP within a short time span.
Dynamic light scattering (DLS) measurements were then performed to monitor particle size distributions during the aggregation of hIAPP. Gradually increased particle sizes were observed during incubation. The average diameter of native hIAPP monomers was 0.1 nm (Fig. 6A), the hydrodynamic diameter increased to 62 nm after incubating for 12 min, suggesting the formation of large size oligomers (Fig. 6B). 36 min later, protofibrils were observed with an average particle size of 525 nm, which further assembled into mature fibrils with an average particle size of 2890 nm at 72 min (Fig. 6C & 6D). This process was significantly accelerated in the presence of 10-fold BPA, the average particle size measured from the first scan was 205 nm (Fig. 6E), significantly higher compared to the companying BPA-free hIAPP sample (0.1 nm), suggested a rapid oligomerization; protofibrils with an average size of 541 nm were observed at 12 min (Fig. 6F) and fibrils with sizes greater than 5000 nm appeared after only 24 min (data not shown). The average particle size reached 10000 nm at 36 min and no more significant change was observed with further incubation (Fig. 6G & 6H). Control studies (containing just BPA as the blank) suggested no interference (signals) from BPA.
The cytotoxic process of hIAPP fibrillation has been shown to decrease the viability of pancreatic INS-1 cells [42]. We found INS-1 cells co-incubated with 5 µM hIAPP for 24 h resulted in a reduced cell viability of 95.9±0.8% (P<0.05) compared with the untreated control cells. And the cytotoxicity was dose-dependently enhanced in the presence of BPA (Fig. 7). Compared with cells treated with hIAPP alone, the cell viability dropped to 85.3±3.2% (P<0.01) at 2 fold molar amount of BPA in combination with hIAPP; whereas at ratios of 5 and 10, it further dropped to 79.2±4.8% (P<0.01) and 62.1±5.3% (P<0.001). No obvious effect was found at BPA ratio of 11 (Fig. 7) or lower (data not shown). Since BPA by itself also showed strong cytotoxicity,CDI was calculated to analyze whether BPA and hIAPP have synergic action on the cytotoxicity. Except the equimolar group, CDIs of the three BPA groups at higher ratio are all below 1, suggesting the existence of a synergistic effect (Table S3). These data indicate BPA may have promotional effect on hIAPP cytotoxicity in certain concentration range.
hIAPP oligomers and mature fibrils have been reported to disrupt β-cell membranes and release intracellular contents, which results in β-cell apoptosis and eventually diabetes [9]. We performed dye leakage assays that use artificial micelles containing fluorescence dye to probe the membrane disruption capacity of hIAPP. The control study suggested BPA alone did not disrupt membranes in the range of 0.5–5 µM, and slight membrane disruption (<6%) was observed in the presence of 10 µM BPA. In contrast, 1 µM hIAPP disrupted membrane by 56.5±0.1% within 90 s (Fig. 8A), which is consistent with a previous report [43]. The presence of BPA in ratios from 1 to 10 all substantially increased the membrane disruption of hIAPP compared to that of the hIAPP alone. In the presence of 2-fold and 5-fold BPA, the membrane disruption was increased to 64.7±0.2% (P<0.05) and 68.9±0.1% (P<0.05), respectively. The 10-fold BPA showed the highest penetration ability of 86.3±0.1% (P<0.001; Fig. 8A).
It has been well documented that amyloid formation is also associated with the generation of toxic ROS [44]. To test whether BPA exposure has any effect on the ROS generation, which is associated with amyloid formation of hIAPP, the ROS levels were measured. Since it has been shown that high concentrations of BPA may directly promote the production of ROS of cells [45], we first treated INS-1 cells with increased concentrations of BPA. Only slightly increased ROS levels was observed at BPA concentrations below 50 µM (data not shown), so 20 µM and 50 µM BPA were used in the following experiments. Compared with untreated INS-1 cells, 10 µM hIAPP caused a 46.2% increase in the ROS level (P<0.05), which agreed with a previous report [46]. For INS-1 cells treated with both hIAPP (10 µM) and BPA (20 µM or 50 µM), the ROS levels raised another 15.6% and 21.1% compared to the cells treated only with hIAPP (P<0.05; Fig. 8B).
hIAPP has a strong tendency to form toxic oligomers and fibrils that lead to pancreatic β-cell apoptosis and eventually the onset of T2DM [47]. It is clear from our results that BPA promotes hIAPP aggregation in a dose-dependent manner, which is supported by the significantly accelerated aggregation lag time as well as the enhanced fluorescence intensity that reflects the orderly β-structures formed (Fig. 3B). CD data further confirms the accelerated transition of hIAPP from unordered structure to β-structure in the presence of BPA (Fig. S2). The helical intermediates are thought to play a role in hIAPP aggregation [48], [49], the transition from helix to β-sheet structure may cause the reduction of helical structures as what we observed (Fig. S2).
It is well established that the toxic hIAPP oligomers can disrupt the islet β-cell membrane and lead to permeabilization. In MTT study, we observed the cytotoxicity of hIAPP on INS-1 cells rose sharply with the addition of BPA (Fig. 7). The observed strong cytotoxicity by BPA alone also agree with previous studies which suggested BPA itself also disrupt the cell function through stimulating the estrogen-receptor and several other apoptosis-related pathways [50]–[53]. It is interesting to note that the increased toxic effect of hIAPP in combination with BPA was only observed at high BPA to hIAPP ratios but not at the lower ratios. The CDI was calculated to explore the potential BPA and hIAPP interaction, and it was found that the two compounds showed a synergistic exacerbation of cototoxicity especially at high molar ratios of BPA.
Further analysis was conducted to distinguish the direct molecular toxic effects of BPA on live cells from its interaction with hIAPP. Dye leakage assays were performed to monitor exclusively the membrane disruption property of hIAPP in the presence of BPA. The hIAPP oligomers have been proven to bind and penetrate membranes more efficiently than monomers and are regarded as an important causative factor to β-cell death [47]. Serious dye leakage was observed in the hIAPP group and was dose-dependently enhanced in the presence of BPA (Fig. 8). It is interesting to note that no evident hIAPP aggregation was identified within a short incubation time at a low concentration (1 µM) as suggested by the ThT and CD results (data not shown), whereas hIAPP at this concentration immediately caused significant membrane disruption in dye leakage assays. This may be explained by a recent report that hIAPP form oligomers much faster in the presence of membrane structures [54]. These data suggested that BPA significantly increases the ability of hIAPP to disrupt membranes.
Oxidative stress induced cytotoxicity was another mechanism underlying amyloid-related β-cell apoptosis besides direct membrane disruption. Amyloid formation has been reported to associate with ROS generation [46]. hIAPP oligomers may form pores on membrane and lead to permeabilization of lipid bilayers [55]. The generalized increase in membrane permeability results in intracellular calcium elevation which disrupts mitochondrial function and finally increases ROS generation [56], [57]. In this study, ROS accumulation is also observed in INS-1 cells treated with hIAPP. The ROS levels rose significantly in the presence of BPA and hIAPP, while BPA by itself has little effect on ROS levels (Fig. 8B), suggesting BPA has a synergistic effect on the ROS production related to hIAPP amyloid formation. A summary of possible molecular scheme is provided for the toxic effects of BPA on the formation of hIAPP amyloid which further result in cell damage (Fig. 9).
The influence on human health of BPA exposure is regarded as an accumulative process because of its widespread penetration in daily life [58]. The BPA tolerable intake has been set as 50 µg/kg/day [58], but adverse effects at lower BPA concentrations in animal studies have been demonstrated, which may lead to the requirement of a new risk assessment for BPA [59]. BPA concentrations in human blood (serum and plasma) are in the range of 0.3–4.4 ng/ml (1.3–19.4 nM) in developed countries [13]. In contrast, physiological circulating concentrations of hIAPP are below 10 pM in fasted non-diabetic people and rise up to over 20 pM after a meal [60], suggesting the physiological ratio of BPA to hIAPP may actually be much higher than those used in the present study. Moreover, since BPA exposure is a continuous and accumulative process, it is logical to expect long-term BPA exposure may be accompanied with accelerated hIAPP amyloid formation and β-cell apoptosis, and eventually a higher risk of T2DM. In a recent report, BPA at near physiological concentration also showed direct toxicity [61]. Due to the detection sensitivity limitation of existing biophysical technologies, in the present study, we tested the interaction between hIAPP and BPA at much higher concentrations in vitro. Therefore the system we used may be considered as a model that simulates physiological interactions at accelerating rates. Future biophysical study with novel experimental methods which can tackle hIAPP and BPA interaction at physiological conditions will be important.
In summary, our data provide the evidence that BPA exposure concentration-dependently accelerates the toxic amyloid formation, exacerbates the toxic membrane disruption of hIAPP and promotes the levels of toxic ROS generated by hIAPP in vitro. Our study suggest that in addition to direct biological effect, long-term BPA exposure may also have adverse effects on hIAPP amyloid formation that eventually contribute to the onset of T2DM. The results may provide a new angle on how BPA exposure influences the risk of diabetes from hIAPP aggregation related pathogenesis. Moreover, since BPA also possess other important biological effects including estrogen-like function, it will be interesting to explore the effect of BPA exposure on physiological functions of hIAPP, for example, hIAPP secretion and hIAPP related insulin resistance. In addition to hIAPP, a great variety of amyloidogenic proteins such as amyloid β peptide and α-synuclein, are known to form extracellular amyloid deposits that induce human diseases including Alzheimer's disease and Parkinson's disease [62]. It will be of future interest to study how BPA exposure may affect the misfolding of those amyloidogenic proteins.
Secondary structure compositions (in %) of hIAPP incubated with different ratios of BPA. The spectra were calculated with the algorithm CONTINLL provided by the CDPro package using SDP42 as the reference set.
Amyloidogenic properties of hIAPP incubated with different ratios of BPA.
Synergistic effects of BPA and hIAPP.
Aggregation analysis of hIAPP co-incubated with BPA and corresponding BPA control. (A) ThT-fluorescence profile over 24 h incubation. hIAPP concentration was 15 µM. (B) Size-exclusion gel filtration profile. (C) TEM images of samples incubated for 4 h.
Percentages of secondary structure contents of hIAPP in the presence of different molar ratios of BPA as calculated by the CONTINLL algorithm. (A) hIAPP; (B) hIAPP with equal BPA; (C) hIAPP with 5-fold molar BPA.
The authors are grateful to the Huazhong University of Science and Technology Analytical and Testing Center for support. The authors wish to thank Dr. Mitchell Sullivan (University of Queensland) and Mr. Justus Grave (Univeristy of Marburg) for help with the editing. The authors thank two anonymous reviewers for their insightful scientific suggestions.
This work was supported by the National Basic Research Program of China (2009BC918304 & 2012CB524901 to LZ), the Natural Science Foundation of China (Nos. 81222043, 30970607, 81172971 to KH; and Nos. 81100687 & 31271370 to LZ), the Program for New Century Excellent Talents in University (NECT10-0623 to LZ & NECT11-0170 to KH). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.

subset: commoncrawl


text:
CITIC Ltd (0267.HK)
28 Sep 2016
Thu, Aug 25 2016
Fitch: China Overseas Land Turns Net Cash Positive; Poised for CITIC Acquisition
(The following statement was released by the rating agency) HONG KONG, August 24 (Fitch) China Overseas Land & Investment Limited's (COLI; A-/Stable) net cash position at 1H16 - its first in more than 10 years - is likely to revert to a net debt position by end-2016 after it completes the acquisition of the property portfolio from CITIC Limited, Fitch Ratings says. Nevertheless, COLI's strong operating cash generation, which helped the homebuilder to turn to net cash position of CNY8.7bn a
BRIEF-CITIC Ltd's unit buys more H-shares in China Citic Bank
* Says CITIC Ltd's unit has bought 149.3 million H-shares in the co between July 7-August 1, taking holdings to 65.5 percent after transaction
China conglomerate CITIC sees H1 net profit down by 40-50 pct
SINGAPORE, July 22 China's conglomerate CITIC Ltd expects its net profit to roughly halve in the first half of the year due to lower property sales, a sluggish stock market and a depreciation of the yuan currency.
BRIEF-CITIC adds holdings in China CITIC Bank
* Says chairman Chang Zhenming resigns due to change in job role
BRIEF-CITIC issues 5 bln yuan worth super short-term debentures
* Says it issued 5 billion yuan worth of super short-term debentures, with a term of 89 days and coupon rate of 2.91 percent
BRIEF-Citic Ltd bought more H-shares in China Citic Bank taking holdings to 64.6 pct
* Says Citic Ltd has bought 137.3 million H-shares in the company between April 11 to May 18, taking holdings to 64.6 percent


text:
Aimee Teegarden
Jump to
Actress Aimee Teegarden is best known as Julie Taylor on the popular NBC drama "Friday Night Lights. " She made her television debut in 2003 with a small role on the police procedural"Cold Case" and would go on to snare roles on the fantasy series "Legend of the Seeker," the family comedy "Hannah Montana," and the teen drama "90210." She's also developed a steady modeling career, and has appeared in campaigns for Hollister, Nike, K-Swiss, Old Navy, and Alltel. In 2006, she was cast as good girl Julie on the drama about high school football in a Texas town, "Friday Night Lights." Teegarden has built her good girl reputation in her personal life as well, being an admitted member of Job's Daughters International, a Masonic-sponsored religious youth group. In 2008, she was dubbed an Honorary Sweetheart of DeMolay International, a similar group for boys and young men. In 2011, Teegarden made progress in her big screen career, playing a supporting part in the latest installment in Wes Craven's horror franchise, "Scream 4," and taking the lead in "Prom," a Disney drama in which she plays an overachieving class president and head planner for the big dance who falls for the school rebel.


text:
Latest Updates
August 29, 2014
One of the two Papuan students tortured or otherwise ill-treated by police has been
released. The other is still in detention and has been charged for his peaceful political
activism in...
July 22, 2014
Indonesia's new President Joko Widodo must deliver on campaign promises to improve Indonesia's dire human rights situation, Amnesty International said.
April 29, 2014
Indonesia's next leader must urgently tackle ongoing violations and repeal repressive and discriminatory laws, Amnesty International said today in a human rights agenda for Indonesia's presidential ca...
February 13, 2014
Millions of domestic workers in Indonesia are at risk of economic exploitation and routine abuse while living in legal limbo without protection, Amnesty International said as the country marks Nationa...


text:
For information on writing about other kinds of. Essay-Writing Services You Can Count On. Custom essay writers with years of experience in academic writing who will analyze your project needs Custom essay writing services uk and conduct in-depth research for your paper able to. It is printed with squares, typically essay on paper essay on paper 200 or 400 per sheet, each
BUY ESSAYS - 100% TOP QUALITY Custom esssays, custom papers, custom essay writing, written essays, buy research term papers custom research paper, essay on paper custom …. 24/7 assistance Buy essays online from the smartest writing company! Congratulations! Your long and winding road to the place to buy essay online has ended happily. Writing about Film . Essayontime. You can always rely on our service and see how easily we cope with any academic. English Composition 1 The Proper Format for Essays. One definition is a "prose composition with a focused subject of discussion" or a "long, systematic discourse". Name essay writing involves essay on paper tireless essay on paper research. Looking for essays or homework help? Need inspiration for your research paper? Anti Essays offers essay examples to help students with their essay writing Professional Custom Essay Writing Service. Find Term Papers and College Essay Examples. These results are sorted by most relevant first (ranked search). Concept papers are summaries of projects or issues that reflect the interests, experience and expertise of the. We write papers to make our customers pleased
Essay on paper
184 990 ESSAYS, term …. it is likely that your procrastination is choose to procrastinate. Division Division, or analysis, breaks a thing down into parts so that (typically in a later essay like the argument/position paper), they can be restructured to form. Any paper topics and subjects for you! Professional Custom Essay Writing Service. This handout describes some steps for planning and writing papers about fiction Jane eyre essay texts. The first step is deciding on a topic. Martin's Press: New York). A classification paper says. Do you find it hard to find the time to write your essays? Do you lack confidence in your essay-writing skills? Essaysexperts. Essays: Organization. The best Short Essay Writing Help will provide you with Short Essay Examples of Short Essay Topics and Short Essay Sample! essay on paper Is this your first time to write a reflection paper? You may have little or no idea how a reflection paper should be like. Name essay is an essay on the subject of a name What this handout is about. Mainly essays homework writting in Do my homework australia English, but also a essay on paper few in other languages. We offer. StudyMode offers book notes, free essays, course notes, flashcards and other tools. I have been grading student essays for many years essay on paper


text:
I have done a search on here and couldn't see anything...
I was curious to know why LHR is considered the "host airport" for London 2012 and the airport where all Olympic traffic is trying to be funnelled through?
Is is simply a case they are using Heathrow as it is considered the "premium" gateway for London and also the main hub for British Airways who happen to be a sponsor?...
The reason why I ask this question is surely airports like STN and possibly LCY are better airports to funnel through Olympic traffic. Heathrow is completely the wrong side and got to navigate around Central London to get to the Olympic site.
While STN on a map is further distance to the Olympic site, it is by far the most convenient and quickest airport to access, with the M11 motorway that runs from the airport straight past the stadium. Also the Stansted Express trains could actually stop at Stratford, as at present they run straight through the station.
From a infrastructure perspective STN also has all the facilities to cope, with a 3000+ meter runway to cope with all aircraft type, VIP facilities the opposite side of the airport from the terminal and with the airports passenger numbers declined by 6 million over the last few years, means there is plenty of runway and terminal capacity.
LCY is the closest airport to the site, but limited by capacity and aircraft sizes.
Even though LHR boasts far more destinations than any other UK airport, surely the majority of Athletes and VIP will be coming in on chartered flights, due to the shear numbers flying in from any single nation.
I initially I thought all the major London airports were official Olympic airports, but a friend who works at LTN has told me they are not allowed to refer to the airport as an Olympic gateway as this title is only reserved for LHR and no other London airport is allowed to refer to themselves as this. While LTN will still have London 2012 help point desk to assist any travellers flying through the airport.


text:
Automotive Los Angeles
Best automotive service in your area
The Automotive Locksmith Los Angeles helps in offering one of the best locksmith services to the citizens of Los Angeles. Our services are carefully designed and altered which helps our customers who are in need of help or are stuck in any kind of emergency. We have the best and trained professionals who provide the services to the people who are in need of help and that too at highly reasonable rates. We have the best technicians who are highly qualified and have experience in the stream of
Requirement of locksmith
Many a times a situation arises in which you have accidently left your car keys in the car and are stuck out. This is the time when the requirement of emergency automotive locksmith Los Angeles comes in. We have the best and most trained technicians who provide 24*7 Emergency serviceto our client who are in need of assistance or help. We provide all our services at highly affordable costs so spending money on the duplication of the keys, getting the car opened or any other related situations does not harms the pocket of our clients.
We have allotted such professional technicians in our company who are completely experts in giving the best of the locksmith services to those who are in need of one. Our trained technicians are so receptive that they reply to the clients query in 15 minute quick response time and serve them with the best solution to their problems. We also train our employees on regular basis to give any sort of emergency services in need so as to build up a good reputation in the clients mind. We have the best technicians in Locksmith Los AngelesCompany which helps in satisfying the needs and wants of those people who are in need of emergencies.


text:
2868 S 26Th Ave,Minneapolis, MN 55406 | Map
The Sprint Network is faster, more reliable with better coverage than ever. Sprint's LTE Plus Net... Read More
10 W Lake St,Minneapolis, MN 55408 | Map
Please visit your local store at 10 W Lake Street in Minneapolis for more details about holiday h... Read More
3. CPR Cell Phone Repair Plymouth
16605 County Road 24,Minneapolis, MN 55447 | Map
Cell Phone Repair Plymouth offers comprehensive repair services ranging from smartphone repair to... Read More
2105 W 80 1/2 St,Minneapolis, MN 55431 | Map
At Cell Phone Repair Bloomington, we're committed to giving our customers the most reliable repai... Read More
8360 10th Ave N,Minneapolis, MN 55427 | Map
Welcome to Mid-Co A/V, an audio/visual company serving clients throughout the Minneapolis, Minnes... Read More
2000 NE Court,Minneapolis, MN 55425 | Map
Please visit your local store at 2000 NE Court in Bloomington for more details about values and d... Read More
7401 Lyndale Ave S,Minneapolis, MN 55423 | Map
Welcome to Audio Perfection!
Audio Perfection proudly serves Minneapolis and St. Paul, MN.... Read More
200 American Blvd W,Minneapolis, MN 55420 | Map
Visit your Bloomington Sam's Club. Members enjoy exceptional warehouse club values on superior pr... Read More
700 Mendelssohn Ave N,Minneapolis, MN 55427 | Map
10359 Zinran Cir S,Minneapolis, MN 55438 | Map
No Box TVs offers top tier, brand name LCD, LED and PLASMA televisions at outstanding prices. Buy... Read More
825 Glenwood Ave,Minneapolis, MN 55405 | Map
Home audio and theater systems designed and installed. Featuring product lines that represent the... Read More
400 Cedar Lake Rd S,Minneapolis, MN 55405 | Map
3009 Nicollet Ave,Minneapolis, MN 55408 | Map
The Sprint Network is faster, more reliable with better coverage than ever. Sprint's LTE Plus Net... Read More
3009 Nicollet Avenue South,Minneapolis, MN 55408 | Map
The new RadioShack is your neighborhood consumer electronics convenience store. Our unique small ... Read More
3018 Hennepin Avenue,Minneapolis, MN 55408 | Map
Visit the Apple Retail Store to shop for Mac, iPhone, iPad, iPod, and more. Sign up for free work... Read More
2868 26th Avenue South,Minneapolis, MN 55406 | Map
The new RadioShack is your neighborhood consumer electronics convenience store. Our unique small ... Read More
2640 Hennepin Ave,Minneapolis, MN 55408 | Map
1701 W 94th St,Minneapolis, MN 55431 | Map
2208 E Lake St,Minneapolis, MN 55407 | Map
Home Choice offers you a whole new way to purchase brand-name home furnishings today! We offer f... Read More
7601 Penn Ave S,Minneapolis, MN 55423 | Map
We make technology work for you with a full selection of HDTVs, computers, cameras, gaming consol... Read More
6667 W Old Shakopee Rd,Minneapolis, MN 55438 | Map
Marine electronics and accessories for the sport fisherman, including color flashers, LCD graphs,... Read More
8450 University Ave NE,Minneapolis, MN 55432 | Map
Visit your newest Walmart pharmacy today. Walmart pharmacies accept major Rx insurance plans, suc... Read More
7316 Aspen Ln N,Minneapolis, MN 55428 | Map
DSG's Electrical Group provides products and services out of 14 branches throughout North Dakota,... Read More
3820 E Lake St,Minneapolis, MN 55406 | Map
6020 Olson Memorial Hwy,Minneapolis, MN 55422 | Map
1 Hour Photo Mart,Minneapolis, MN 55428 | Map
315 7 Ave N,Minneapolis, MN 55401 | Map
ABC Electronics located in Minneapolis, MN, is an electronic part store. We sell and buy electron... Read More
8150 University Ave NE,Minneapolis, MN 55432 | Map
Visit your Fridley Sam's Club. Members enjoy exceptional warehouse club values on superior produc... Read More
609 W 74th St,Minneapolis, MN 55423 | Map
2526 Central Ave NE,Minneapolis, MN 55418 | Map


text:
سكس موفي سكس جا مد So why does an engine with huge torque and low maximum RPM get a low horsepower rating? If you have also read the article entitled How Horsepower Works, then you know that one horsepower is also equal to 33,000 foot-pounds of work per minute. سكس كاميرون دياز Gently breathe out of your mouth on the cheek/neck, and listen to them giggle and groan with delight! Asian music encompasses numerous different musical styles originating from a large number of Asian cultures. As such, they would not also re-air Miller�s various HBO specials. سكس موفي By this measure, one horse can raise 33 pounds 1,000 vertical feet in a minute, or 330 pounds 100 feet in a minute, or 3,300 pounds 10 feet in a minute, and so on. سكس كاميرون دياز Your 11 liter diesel engine has a long stroke. Angel Kiss - A sweet, comforting kiss.
Over time, the width of the hem grew wider and the term `flare-leg` was favored in marketing over the term `bell-bottom`. The hip-huggers of the 60s and 70s can be distinguished from those of the 90s by the tightness of the knee.8-second, almost 11 seconds quicker than it takes a production Porsche 911 Turbo to reach the same speed? � that an NHRA Top Fuel dragster leaves the starting line with a force nearly five times that of gravity, the same force of the space shuttle when it leaves the launching pad at Cape Canaveral? � that an NHRA Funny Car is slowed by a reverse force more than seven times that of gravity when both parachutes deploy simultaneously?Breakout: Used only in handicap racing, `breakout` refers to a race car running quicker than the driver has predicted. In most classes, a burnout precedes every run down the dragstrip.
سكس نساء مع حصان that should not make him stop begging. These tend to be the shows that are original stories, but the vast majority are pitched by the sponsors, which could be the manga publisher, who`s out to use the anime to promote merchandise or the original manga, or a media company like Media Factory or Geneon Japan or Bandai Visual, who are interested in using the TV broadcast to promote the eventual DVD release.


text: Cory K. wrote: > *Terminator* - https://launchpad.net/terminator > > -Terminator, multiple terminals in one window. This is a little project > to produce an efficient way of filling a large area of screen space with > terminals. > > "The robot future of terminals." > > > This is a project started by our very own Canonical sysadmin > extraordinar Chris Jones. (Ng on Freenode) > > He's looking for a custom terminal icon so he can stop using the GNOME > one. :) > > Ideas can be anything. Wide-open. Work in the multi-window aspect > somehow I would say. > > The final icon will make the Hardy release of Terminator. Not 1 response in here? :) -Cory \m/


text:
Showing available accommodation2 guests USD Change dates
Sorry, we couldn't find any available accommodation that matches your search criteria.
Try removing some filters.
Beau Vallon and Bel Ombre offer the widest range of accommodation in north Mahé, with a good mix of luxury resorts and self-catering villas set on or just back from the lovely beach.
Try removing some filters.


text:
Top Definition
When A Man Is Half-Erect.
Look At Tommy, He Is Half-Staff. Will It Do The Trick For His Girlfriend?
de Kevin 26 janvier 2004
Trending Now
Can you define these popular missing words?
To have a half boner or chubby
When you think a hawt girl is looking at you u might be Half Staff
de J Stroke 11 octobre 2009
A pejorative term for a bisexual
"Uh oh, Tony's acting the half-staff again. Crypto-dyke."
de Wangbacca 5 février 2009


text:
coumadin toxicity icd 9
You are responsible for what you say
coumadin online purchase
coumadin dose adjustment algorithm
It's also important to remember that any drug that has a measurable effect, even if inhaled from a traditionally prepared essential oil, may also have a measurable side-effect
chest guidelines warfarin initiation
warfarin dosing nomogram 2012
Audrey's organs began to shut down and she was put into a medically induced coma, the doctors telling Marc they didn't know if she would survive.
order warfarin
coumadin diet list in spanish
"Racial stereotypes lead some to believe that the Chinese are taking all of the good jobs and earning more money than Malays themselves," she says
warfarin dosing for pulmonary embolism
warfarin drug interactions with alcohol
"Plaintiffs have failed to allege that Apple's logic boards were unfit for their ordinary purposes or lacked a minimal level of quality," Alsup wrote
how does warfarin interact with food
list of foods not to eat when taking coumadin
We need everyone talk about organ donation with their families and agree to donate if ever they are asked.
drug interaction bactrim and warfarin
coumadin diet patient handout
buy coumadin canada
Today's announcement adds to the investment and programmes we have put in place.
diet restrictions for patients on coumadin
A Scottish Widows spokeswoman said: "The Which? analysis is inaccurate and significantly misrepresents our charges
vitamin k and coumadin food list
coumadin and plant based diet
In an Ask Me Anything thread on Reddit, an anonymous McDonald's manager has confirmed the existence of the fast food chain's so-called secret menu
coumadin toxicity treatment guidelines
A key risk factor will be how much a bank relies on short-term funding markets to borrow from other banks
warfarin pt inr range
Zweig coined the term "aces" for the three new hypothesised building blocks, but it was Gell-Mann's name "quark" that stuck.
list of foods not to eat while taking warfarin
coumadin interactions with other medications
coumadin interactions
warfarin inr guidelines uk
coumadin inr levels normal
coumadin warfarin buy online
It's probably the day that will decide the Tour.
coumadin side effects after stopping
how does warfarin interacts with other drugs
This involved almost 4,000 children and their mothers from 34 different studies.
interaction between warfarin and alcohol
By then aircraft might be landing like helicopters or spaceships or something we can't even imagine
warfarin dosage for pe
dietary restrictions for patients on warfarin
aha coumadin dosing guidelines
The reality is that the diminishing size of the regular military, a result of relentless defence cuts, means that the recruiting pool for the SAS and SBS has in turn diminished
coumadin and alcohol use
inr values on coumadin
list of foods you should not eat while on coumadin
Research carried out for the campaign found that less than 10% of young women take the vitamin, while one in 10 believe they can get enough of it from food
list of foods not to eat when taking warfarin
drug interaction between warfarin and bactrim
viagra and coumadin interaction
coumadin diet teaching sheet
diet plan while on coumadin
coumadin toxicity definition
It can't be more than a few days after the leak that someone builds a tool allowing you to see which of your Facebook friends have signed up.
therapeutic inr levels warfarin
coumadin levels and exercise
heart disease coumadin and alcohol consumption
His muscles had contracted so much that he struggled to get the length needed to drive through the water
coumadin toxicity symptoms
Gatter:[laughs] Exactly I wouldn't know how to drive a Lamborghini
order coumadin booklet
normal inr for coumadin therapy
coumadin dosing chart
cheap coumadin
icd 9 cm code for coumadin toxicity
coumadin without a prescription
Many have suggested that he'd be the ideal man to fix Barclays
can i mix coumadin and alcohol
list of foods you can eat on warfarin
coumadin and alcohol mayo clinic
coumadin meters for sale
coumadin dosing based on inr
normal inr range without warfarin
Its forests and savannas are ravaged by wildfires because fire season changed dramatically in the last 35 years.
warfarin and alcohol side effects
drug interactions--coumadin and viagra
coumadin diet handout pdf
normal inr lab values on coumadin
warfarin initial dose calculator
That, too, he skewed, sending it in a wild loop over the heads of the watching crowd
damiana e coumadin
generic coumadin names
"It would require companies to approach HMRC about any uncertainties, and HMRC would then decide what Parliament would have intended under the law
does coumadin interact with vitamin c
coumadin normal levels inr
She was mildly duplicitous, encouraging a doomed project perhaps without telling its makers it was doomed, gossipy about salaries and billing
inr range when on coumadin
That fanbase really got F'ed when Colorado became a powerhouse right when they left
coumadin inr
buy cheap warfarin online
what happens if coumadin level is too high
coumadin anticoagulation guidelines
Johnson was not expected to be a key contributor for the Seminoles in 2015, but Cook is considered one of the top running backs in America
coumadin levels inr range
coumadin and alcohol
coumadin diet handout patients
So there might be something else going on that doesn't just relate to their penis."
coumadin warfarin buy
chest guidelines coumadin dosing
"With the soccer players it may be slightly different since few of Cuba's soccer players are big name prospects who will demand high signing bonuses from pro teams.
buy cheap warfarin
Longridge News provides news, events and sport features from the Longridge area
coumadin inr levels
buy coumadin tester
dr. gourmet coumadin diet pdf
Entering Sunday, he had been 12-1 in 20 starts since 2014 when getting three runs or more of support.
coumadin interactions with food and drugs
coumadin levels normal range
A cliché, but a powerful one – because it's true
warfarin anticoagulation reversal guidelines
dietary recommendations for patients on coumadin
warfarin pt/inr levels
inr levels on coumadin
can i take warfarin and ibuprofen together
coumadin lab levels
order warfarin online
purchase coumadin online
coumadin and alcohol consumption symptoms
warfarin dose for pulmonary embolism
Some in Greece were sceptical that the move to reopen banks would change much
inr levels coumadin normal range
vitamin k rich foods list and coumadin
While performing at Manchester Arena on Sunday, the girls put on an energetic performance in a range of stylish grungy outfits.
coumadin initiation dosing algorithm
pt inr coumadin values


text:
Reviewed By Jeffrey M (Durham, NC) Reviewed for a 2011 Ford F-150 —6/20/2012 9:35 AM
The product could be a little better quality, but it works as advertised.
Reviewed By Robert G (Staunton, VA) Reviewed for a 2017 Chrysler Pacifica —8/20/2016 2:37 AM
Bought this to fill in the area between carpeted mats when the seats are stowed in my new 2017 Chrysler pacifica. Had to trim but no problem. Wish it was thicker but it really does just what I wanted.


text: Posted July 5, 2011 The Royal Ballet has a school and so does the New York City Ballet. American Ballet Theater has just started a school but it is too new and the students are too young to create a new generation of stars right now. The other oddity about the Royal Ballet for several decades now is that most of the star dancers are foreigners who trained elsewhere. Acosta (Cuba), Cojocaru (Romania/Kiev), Marianela Nunez (Argentina), Rojo (Spain) and all those Italians, Russians, Brazilians, etc. Former principal dancers Sylvie Guillem (poached from POB) and Alexandra Ansanelli (poached from NYCB). There are very few English dancers perpetuating the Ashton/de Valois English school of ballet who are products of the RB school. To my eyes, Darcey Bussell is the last and she was sort of an atypical English ballerina - more American in her physical attack and boldness. Where is the younger than 35 or 40 year old English ballerina with that kind of star power? Royal Ballet School needs to redress this. However, compare the NYCB roster with ABT's - most of the principals are young, fresh and trained at SAB home-grown product. As for Chase Finlay - he ain't going nowhere, he is in the right place at the right time. As for comparisons with the ABT and NYCB roster - this has changed a lot in 20 years. I actually find that NYCB is crazy strong in superb ballerinas right now (Mearns, Scheller, Reichlen, Bouder, Fairchild, Peck and on and on) but the male contingent is functional but not brilliant. I like Gonzalo Garcia and De Luz. To my eyes neither Jonathan Stafford or either Angle brother can compare with the best dancers circa 1991 when I started attending ballet in NY. When I started going to NYCB in the early 90's they had a better male roster than ABT. ABT had the young phenom Julio Bocca just coming into his own as an artist. The rest were functional (Wes Chapman, Ross Stretton), short-lived (Ricardo Bustamante) or veterans finishing up their dancing careers (Kevin McKenzie, Fernando Bujones, Johan Renvall, Danilo Radojevic). Guest stars were coming in, especially under Jane Herrmann, such as Andris Liepa and Faroukh Ruzimatov (the Soviet bloc was opening up thanks to Perestroika). Guillem guested and so did veteran Russians like Maximova and Vasiliev and the lovely Lyudmila Semenyaka. However, compared with the company 10 years before when young Bujones, Baryshnikov et al. ruled the stage, small potatoes. In comparison, NYCB had the young Peter Boal, Damian Woetzel, Nicolae Hubbe (a few years later), Albert Evans, Jock Soto along with still excellent veterans like Adam Luders and up and comers like Philip Neal. From 1990 to 1995, NYCB had the best male principal roster, much better than ABT. Then Kevin McKenzie went on a spending spree and bought and brought in José Manuel Carreno, Vladimir Malakhov, Maxim Belotserkovsky, Angel Corella, Ethan Stiefel, Joaquin de Luz and added them to the still going strong Julio Bocca. Suddenly there was a killer superstar male contingent there. The female roster was not as quickly refreshed. Veteran prima ballerinas Susan Jaffe and Amanda McKerrow were revitalized and inspired by their partnerships with Carreno and Malakhov respectively. But Gillian Murphy was the big ballerina discovery from the ranks. Herrera was already on the rise and artistically plateaued. Ananiashvili was acquired as an established superstar. But McKenzie never had the eye for ballerinas that he did for male dancers and he has coasted on his "Born to Be Wild" contingent for too many years. Hallberg and Gomes are holding up the company as their successors but where are their successors? That male principal star explosion was 16 years ago and time has taken its toll. Some like Malakhov were pushed out when they had too many outside commitments - Malakhov one season was only offered a matinee "Swan Lake" and that was it. Corella clearly has been receiving the same treatment from ABT management. Most have simply aged out and retired. Or aged out and didn't retire... De Luz moved to NYCB due to personal stresses and repercussions from his divorce from Carmen Corella. Now there seems to be a lack of brilliant new male principals in both companies. Antonio Carmena and Chase Finlay are rising up and comers. Craig Hall is a very fine underutilized dancer. But Martins has a new scintillating crop of young ballerinas who can thrill in the entire repertory. Finding men who match their level of brilliance is difficult. So it looks like superstar quality men are in short supply worldwide and there is more demand than supply. Also, major international companies have always, always lured star dancers from the competition, it has been standard practice for decades. Even the Bolshoi and Kirov-Mariinsky have been known to snatch up good men from Kiev, Perm or wherever when their own conservatory product doesn't live up to expectations or proves inadequate. Share this post Link to post


text:
In-Sink-Erator FHC1100BIS
F-HC1100BIS Series 1100 Instant Hot & Cool Water Dispenser in Biscuit
Available Usually Ships within 24-48 Hours[Only 5 available]
Quick Specs:
- Width: 5 1/2"
- Height: 8 3/8"
Learn more »
Indulge Contemporary is our finest line of instant hot water dispensers for use with our Stainless Steel Tank for filtered near-boiling 200 F water and cool drinking water all in one system. The contemporary design, in an array of beautiful designer finishes, will complement any kitchen. Available at AppliancesConnection
InSinkErator's Instant Hot Water Dispenser saves time and adds elegance to the kitchen. Near-boiling water at your fingertips -- perfect for making gravy, melting chocolate or quickly preparing pasta. Also double-functions to remove candle wax from holders and quickly clean dirty pots and pans.
Consider how often you heat water. When you do, you'll realize just how much you would use an instant hot water dispenser. It's truly unrivaled in the kitchen when it comes to the number of tasks that are easily and quickly performed. And that's exactly why millions of people have installed them.
Hot Valve: Instant, self-closing
Cold Valve: Manual return
Shipping Weight: Approximately 4.0 lbs.
Required Hole Diameter: 1 3/8" - 1 1/2" (standard)
- Brand:In-Sink-Erator
- Stock Id:34690
- Color:Bisque
- Width:5 1/2"
- Height:8 3/8"
- Approximate Shipping Weight:Approximately 4.0 lbs.
- Hot Valve:Instant, self-closing
- Required Hole Diameter:1 3/8" - 1 1/2" (standard)
- Cold Valve:Manual return
Technical Details
Shipping Methods & Details
- Please enter your shipping ZIP Code to get available shipping methods and cost:


text:
Already a member? Log in.
Help support your Meetup
Hi I am starting this group for people who would like to enjoy outdoor activites and looking to make new friends along the way. If you like to go camping, fishing, crabbing, dancing, movies, dinner, concerts, beaches, traveling then this would be the group for you to join.
Discount on moving services home/office. Mention CJFG receive discount
2,299 Friends
3,988 Members
2,253 Trail shrinks
4,139 Doers
1,036 Dancers and Socializers
2,164 Dancers and Socializers
Meetup members, Log in
By clicking "Sign up" or "Sign up using Facebook", you confirm that you accept our Terms of Service & Privacy Policy


text:
Pure linen quilt cover set - deep sea
Pure linen quilt cover set vintage washed for ultimate softness & luxury. Breathable & naturally hypoallergenic. Designed locally in Australia. Available in standard bed sizes, please make your selection via the dropdown menu.
Buying this product = 289 Reward points for Members. Your cart will total 289 points = voucher of $14.45. Not a Member? Join at checkout to claim these points.
Customer ratings and reviews


text:
Cookie Salute
Additional Information
Step 1
Prepare and roll out cookie dough. Cut with flag cutter. Bake and cool cookies.
Step 2
Pipe on icing using tip 8; smooth with spatula. Use blue gel to fill in upper corner; sprinkle with white nonpareils.
Step 3
Pipe red gel wavy stripes. Pipe tip 6 line for pole and dot on top.
In Stock (ships in 1-2 business days)
Golden Yellow Icing Color
Red Sparkle Gel
Blue Sparkle Gel
In Stock (ships in 1-2 business days)
In Stock (ships in 1-2 business days)
Cookie Sheet
Cooling Grid
Item(s) added to the shopping cart!View Shopping Cart Continue Shopping
Item(s) added to your Favorites!View Favorites Continue Shopping
You Might Also Like
Show your true colors! Create a flag-waving cake topped with a bottle-rocket display of sparkling cookies shaped by Stars Nesting Metal Cutters.
ROTC and military-academy graduates are sure to salute these playfully patriotic petit fours. Bake six at a time in our Mini Star Pan, cool, and cover with melted Candy Melts. Finish with Patriotic Nonpareil Sprinkle Decorations and piped candy details.
It´s red, white and blue through and through! Cake batter is tinted before baking and the colorful layers sandwich a sheet of luscious ice cream. The coolest summer cake ever. Watch our online video.


text:
Dodgers? Giants? Don't overlook the Diamondbacks in the NL West.
The Diamondbacks have trimmed their deficit in the NL East from 9 games to 6 1/2 in just the last few days. But they've been playing well for awhile now, winning 11 of 16 and 20 of 33. Lately, their offense has been throwing a lot of runs on the board–46 in the five games before today's contest against the Cubs. They're leading the Majors in runs since June 5. In addition, their .998 fielding percentage if baseball's best.
Manager Kirk Gibson pushed his team hard on fundamentals the last two springs. One byproduct was back-to-back lousy spring training records. Another is that the Diamondbacks are as fundamentally sound as almost any team in baseball. Gibson still has his players on the field early some days to work on the basics. Hard to argue with the results.
Gibson just used the same lineup for consecutive games for the first time this season. One of his most productive arrangements has been at first base, where Paul Goldschmidt and Lyle Overbay have combined to hit .310–only the Reds have a higher batting average at first–with 40 extra base hits (second) and a .389 OBP (third).
The Diamondbacks have gotten solid work from their bullpen. It held opponents scoreless in 11 of 18 games this month, with a 3-1 record, three saves and a 3.47 ERA.
His remaining headache is in the rotation, where Daniel Hudson has a 6.60 ERA and Ian Kennedy had a tough start . Short starts have left a good bullpen taxed. Hudson was part of Arizona's rise last season, going 16-12 with a 3.49 ERA and 222 innings.With Joe Saunders on the disabled list, fans are clamoring for 2011 No. 1 pick Trevor Bauer to get a shot, but he has allowed 60 base runners in 42 innings at Class AAA. His broader numbers are outstanding: 2.79 ERA with 51 strikeouts in 42 innings. He might also benefit from better competition because he surely feels he's ready for the Major Leagues.
Regardless, after a very slow start (just like last season), the Diamondbacks look like a team that'll be competitive for the long haul.


text:
From Airbnb to Couchsurfing, Carpooling to Kickstarter – the sharing economy is booming and startups from all areas are emerging to offer peer-to-peer services. Newly-launched Android app CrowdRoaming is one of them. It wants to solve a very common (and frustrating) problem experienced by travellers: huge mobile surfing costs when overseas.
Scooped by
jean lievens
onto Peer2Politics


text:
26090 Euclid Ave
Euclid, OH 44132
25890 Lakeland Blvd
Euclid, OH 44132
We have been taking our cars to Millennium for over two years now. We have always received nothing but the best service from this...
I had to take my car into this auto repair shop back a few weeks ago, and they did a great job. The customer service is wonderful...
©2016 Cox Search, Inc. All rights reserved. Data provided in part by Localeze and Valpak.


text:
Login Screen/Tables/validation for POS
This project received 2 bids from talented freelancers with an average bid price of ₹9500 INR.Get free quotes for a project like this
Skills Required
Project Budget₹1500 - ₹12500 INR
Total Bids2
Project Description
Development of POS system in [url removed, login to view]+MS sql Express; Initially we need Masters - User, Company and validate user as per company. We need coding as per attached details. Job includes creation table; screen design for user input; validate the user ; Page Title is from Company Name after Login.
Looking to make some money?
- Set your budget and the timeframe
- Outline your proposal
- Get paid for your work
Hire Freelancers who also bid on this project
Looking for work?
Work on projects like this and make money from home!Sign Up Now
- Forbes
- The New York Times
- Time
- Wall Street Journal
- Times Online


text:
TOTAL PRO™ Exterior Acrylic Flat
TOTAL PRO Exterior Acrylic Flat is a professional quality, exterior, 100% acrylic flat paint. Its matte flat finish maximizes uniformity over rough, irregular surfaces. It is used for all types of new or previously painted commercial, institutional and residential exterior wood and masonry surfaces such as siding, trim, eaves, facias, shutters, fences, properly prepared brick, block, stucco and concrete surfaces, vinyl, primed metals and metal siding, vinyl siding and trim.
Fields denoted with an asterisk (*) are required.
By providing your personal information above, you agree to the collection, use and disclosure of the personal information you provided as described in our privacy policy.
Thanks for signing up to receive updates, offers and promotions from PPG Paints™. We'll be in touch!


text:
1. Before Will and Amy…
Now divorced, that means no more artistic photographs in dope white jackets…
2. …And Megan and Nick…
Karen Walker and Ron Swanson I CAN DIG IT.
3. There was a comedy power couple that overshadowed them all: Gene and Gilda.
4. Take the original Willy Wonka from Willy Wonka & the Chocolate Factory…
Yeah, the guy from that popular meme.
5. Pair him with one of the original cast members of "Saturday Night Live"…
6. And you get COMEDY GOLD.
7. She won an Emmy for playing characters like Roseanne Roseannadanna.
8. He was neurotic Leo Bloom in The Producers.
He's the one with the blanket…
9. They met on the set of Hanky Panky and in her book Gilda said it was "love at first sight."
On the set of another film, "Haunted Honeymoon."
10. Now let's all take a minute to admire their adorableness.
12. They were Instagram BEFORE it even existed.
13. Jim in Blazing Saddles + Emily Litella.
15. Baba Wawa + Dr. Frankenstein.
17. Sadly, Gilda Radner passed away in 1989 at the age of 42 from ovarian cancer.
18. Since then, her book "It's Always Something" has inspired many, as does her excellent work in comedy and theater.
19. Gene Wilder has since remarried and has shifted his focus to writing novels.
20. But in our hearts and memories, they'll always be the ultimate comedy power couple.
This post was created by a member of BuzzFeed Community, where anyone can post awesome lists and creations. Learn more or post your buzz!
- Donald Trump's home near Washington, DC has security cameras inside and out, monitored from New York, insiders say.
- The hair vitamins promoted by the Kardashians make "largely inaccurate" nutrient claims, according to lab tests.
- Donald Trump accused former Miss Universe Alicia Machado of being in a sex tape, calling her "disgusting." He's appeared in a softcore porn.


text:
Berlin business is hit with fine
BERLIN The owner of a tire recycling business on the Barre-Montpelier Road has been slapped with a $17,000 fine for operating a solid waste facility for the past eight years without a permit.
Fred Budzyn, owner of Budzyn Removal & Recycling in Berlin, was fined $17,301 after Environmental Law Judge Thomas Durkin concluded the discarded tires Budzyn was paid to recycle, reuse or dispose of constituted solid waste.
Durkin arrived at that conclusion after a one-day trial that capped the state Agency of Natural Resources repeated but unsuccessful attempts to persuade Budzyn to resolve the violation by obtaining the certification officials said he needed.
According to agency officials, Budzyn has since applied for a solid waste certification and it is expected one will soon be issued now that a financial responsibility instrument designed to ensure cleanup of the facility is in place.MORE IN This Just InMORRISVILLE Lily Metzler (three goals) and Jamie Barrett (two goals) and Sarah Davis (two... Full StorySOUTH BURLINGTON Police are investigating the discovery of a body in a wooded area in South... Full StoryBen & Jerrys
plan expansion
ST. Full Story
- Most Popular
- Most Emailed


text:
Order Cheap Lexapro OnlineFind discount insect repellant patch online find discount insect repellant patch online, order no rx rumalaya find cheap french red wine online order discount toradol online energy patch in canada find himplasia, ultimate viagra pack (viagra + viagra soft tabs + viagra oral jelly).
Order cheap lexapro online find discount cha de bugre cheap neurontin online order prozac online buy himplasia online biklin.
Zoton find cheap lexapro online simvador) find dermavitol online metformin in canada cozaar overnight delivery find cla online find womens intimacy enhancer cod, order discount tramadol online buy cephalexin cod.
Find discount tramaden online.
Penis enhancer.
Deluxe whitening system with plasma lamp in us.
Zithromac order discount weight loss online depo-medrol in canada order cheap chromonexin, find cheap doxycycline online elocom.
Order cheap orlistat online find discount styplon online.
Find discount daily best chews dogs online find cheap penis growth oil alphapril order discount creatine-1200 buy discount beconase aq, travatan order cheap male potency patch, allopurinol in us find ophthacare online.
Order virility gum cod, free noroxin order no rx pain relief patch.
Find male enhancement oil no rx required, order discount metoclopramide, dulcolax overnight delivery lumigan no rx hcg pregnancy midstream test order cheap lexapro online.
Female sexual oil in canada no rx claritin.
Order cheap pletal.
Buy insect repellant patch in canada order cheap plavix online, bisacodyl.
Decadron overnight delivery buy flovent.
Cheap ephedraxin online torsemide in us, find imdur.
Buy discount elavil online myambutol free delivery inflammatory bowel disease order no rx clozaril buy mycelex-g in us lipotrexate in canada find pyruvitol online.
Buy cheap celadrin online, order no rx ampicillin gasex overnight delivery.
Buy cheap melatonix sleep aid, buy omega 3-1 no rx required, order discount ophthacare online sempera buy no rx zestoretic capoten free delivery, find arimidex, order cheap lexapro online, cidomycin, find discount ayurslim online, buy ponstel.
Phosphatidylserine online review order no rx hyzaar buy cheap hydrea online order corticyn trimplex cod order cheap ethionamide find cheap singulair find discount zofran online mircette free delivery buy no rx glyset order chloromint cod hypoten, shuddha guggulu, buy cheap phenergan online free digestive support cats, order soma no rx required.
Order neurontin no rx required optimycin osteoporosis prevention, order discount glucotrol xl online buy discount flonase topgraf xenical find prozac cod shallaki overnight delivery, order septilin glibenclamid order cheap triphala online free synaral acai berry extract, order detrol online zovirax chronic stable angina tritace, find discount 3 panel drug test (thc/coc/met) buy capoten in canada order no rx dermavitol buy discount colostrum-800 online, buy no rx parlodel.
Find no rx echinacea buy cheap coumadin.
Buy discount trazodone.
Bipolar disorder.
Bodybuilding, metabo925 free sample gilex, free albendazole testo-rex free sample, free clavamox buy discount synthroid online find erexor online buy discount tagamet, order discount noxide online equinorm find discount vasotec buy vytorin in us roxin.
Atosil buy amino mass online.
Find panmycin cod no rx ginseng, sleeping aid buy digestive support cats no rx required buy geriforte cod find cheap copegus online.
Gastrosil find cheap mexitil, order no rx didronel order discount himplasia cheap metabo extreme online find no rx motrin skin & coat support dogs free sample find discount l-taurine find cheap extendaquin online order no rx voltaren.
Order keppra, vytorin free delivery.
Free seroquel buy cholestoplex in us hoodia weight loss gum in us apcalis sx (cialis), find cheap cephalexin gluta-pep free delivery, order aleve online buy tetracycline online.
Buy bactrim in canada.
Protein conditioner (softness & shine) calming chews cats online review.
Buy discount infusium topical.
Buy early pregnancy strip test celexa overnight delivery buy methotrexate, order cheap melatonin online buy benemid online cheap nutridrine online, ambroxol miglitol, find premature ejaculation cure order depakote online order discount hytrin online


text:
Hi all.
I am about to take a Chinese domestic trip lasting about three hour, and would like to have your opinions on the two different airlines I have to chose from. If the price is the same, would you chose China Southern or Air China ? Why ? Any previous experiences perhaps you could tell me about ? Thanks !


text:
Brighten a country-style kitchen with this industrial pendant light bathed in a stunning chrome finish.
Add a bold new look to your home decor with this chrome pendant light from the Possini Euro Design lighting collection. Industrial and modern style pair with the best of the past for a stunning look. Great for entryways, dining areas, and more.
Shop all Possini Euro Design
Free Shipping on Most Orders
Our associates are standing by to assist you.


text:
Rutherford College Jobs
Looking for jobs in Rutherford College? Snagajob has great opportunities with the area's leading companies. And applying for Rutherford College jobs has never been easier. Look at the job listings below, click on a job title that interests you and hit the "apply now" button. Part-time jobs, teen jobs, student jobs and summer jobs are now just a click away. Start your job search now.
Rutherford College, NC Job Openings
CRST ExpeditedRutherford College, NC, 28671
Rutherford College, NC
Cities within 20 miles of Rutherford College, NC
- Hickory, NC (339 jobs)
- Morganton, NC (109 jobs)
- Lenoir, NC (66 jobs)
- Conover, NC (34 jobs)
- Hudson, NC (32 jobs)
- Newton, NC (27 jobs)
- Granite Falls, NC (21 jobs)
- Valdese, NC (10 jobs)
- Hildebran, NC (8 jobs)
- Icard, NC (7 jobs)
- Connelly Springs, NC (3 jobs)


text:
Deathtrap, London
Simon Russell Beale has clearly decided to have some fun this year: he's been larking around in London Assurance with Fiona Shaw at the National, camping it up at the Sondheim Proms, and filming for Spooks. Now the actor who's acclaimed for his many classical theatre roles is starring in Ira Levin's comedy thriller Deathtrap (made into a 1982 film with Michael Caine and Christopher Reeve). There's a strong team for an admittedly lightweight piece in director Matthew Warchus, who disinterred the 1960s farce Boeing-Boeing to great acclaim; Estelle Parsons, an Oscar-winner for Bonnie And Clyde, although best known as Roseanne's mum in the TV sitcom; Glee star Jonathan Groff, who starred in the Broadway production of Spring Awakening; and Claire Skinner of TV sitcom Outnumbered.
Noël Coward Theatre, WC2, Sat to 22 Jan
Mark Cook
Spring Awakening, Edinburgh
After a sell-out season at the Lyric Hammersmith, Steven Slater and Duncan Sheik's musical version of the 19th-century tale about a group of German teenagers who discover that ignorance kills, didn't hack it in the West End. A pity, but I reckon that this production by One Academy Productions, featuring graduating students from the Royal Scottish Academy of Music And Drama and a contemporary indie rock soundtrack, will be a massive Edinburgh hit. With an eager young cast and a budget that probably wouldn't have bought a round of drinks for the West End production team, this Scottish premiere should pare back the show and, in the process, up the impact of this tale of adolescent passion and sexual awakening.
Pleasance Courtyard, Sat to 30 Aug
Lyn Gardner
Vieux Carré, Edinburgh
Tennessee Williams's 1977 play took a real pasting on its premiere, being dismissed as autobiographical self indulgence and like an undergraduate parody of his own earlier plays. Like many of Williams's stories, Vieux Carré is full of ghosts, in this instance the inhabitants of a rundown New Orleans boarding house. They include a young man who goes under the name of "the writer", who is clearly a thinly disguised self-portrait of Williams himself. But this little-known play has come to the attention of the Wooster Group, those New York avant gardists who have reimagined the literary visions of Anton Chekhov, Eugene O'Neill and Gertrude Stein to good effect. They now look to create a new mode of expression for Williams's lyrical voice, which draws upon the movies of Paul Morrissey, best known for his links to Andy Warhol, and the media work of American artist Ryan Trecartin.
Royal Lyceum, Sat to Tue
Lyn Gardner
Clybourne Park, London
American writer Bruce Norris garnered a selection of fine reviews for his dark suburban comedy about phoney US liberalism, The Pain And The Itch, in 2007. Now he returns to the Royal Court with the European premiere of Clybourne Park, a piece about race and comedy that opened in New York earlier this year. It's a satirical play of two halves, the first set in Chicago in 1959; the second in 2009. In the earlier period a couple sell their home cheaply, enabling the first black family to move into the cosy white neighbourhood of Clybourne Park. Fifty years on, the same home is being bought by a couple who want to knock it down and start again, raising the hackles of the neighbours in the same way as half a century before. The play is a response to Lorraine Hansberry's seminal A Raisin In The Sun. The cast, who play different parts in each act, includes Sophie Thompson and Martin Freeman, recently seen in TV's terrific Sherlock as John Watson. Dominic Cooke directs.
Jerwood Theatre Downstairs At The Royal Court, SW1, Thu to 2 Oct
Mark Cook
Caledonia, Edinburgh
In 1698 the Scottish speculator William Paterson had a dream of founding a Scottish enclave in Darien in Central America with the aim of turning the poor nation of Scotland into a rich colonial power. He invited people to invest in the scheme and they did in their thousands. But the dream very quickly turned into a nightmare. What began as a heroic voyage to the new world ended in disaster, with a loss of 2,000 lives and a quarter of the nation's wealth. Just nine years later the Treaty of Union was signed with England, and Scotland was no longer an independent nation. The suggestion that the collapse of the Darien scheme played a part in the signing of the Treaty is explored in this latest play written by Alistair Beaton, directed by Anthony Neilson and co-produced by the National Theatre Of Scotland and the Edinburgh International Festival.
King's Theatre, Sat to Thu
Lyn Gardner
The Gospel At Colonus, Edinburgh
Those who have followed Jonathan Mills's reign as artistic director of the Edinburgh International Festival will know he's is a big fan of US director Lee Breuer. This latest show sounds like a real rabble rouser as it transfers Sophocles's tragedy, Oedipus At Colonus, to modern America with a little help from a Pentecostal preacher, the Blind Boys Of Alabama, the Legendary Soul Stirrers, and an Abyssinian baptist choir. It arrives on these shores trailing clouds of critical glory.
Edinburgh Playhouse, Sat to Mon
Lyn Gardner
Our Day Out, Liverpool
It's a long run for Willy Russell's comedy musical about a school trip to Alton Towers undertaken by Mrs Kay's "Progress Class", but that's because this revival was a big hit at this address last year. Originally written as a TV play in 1976, it first made the transition to the stage in a 1983 production by Bob Eaton, former artistic director of the Liverpool Everyman. He's back at the helm for this helter skelter ride. So all aboard: just don't eat your sandwiches before the interval.
Royal Court, Fri to 9 Oct
Lyn Gardner
The King Of Prussia, nr Truro
In Cornwall's Prussia Cove in the late-18th century, brandy smuggling is a way of life. Particularly for John Carter, the self-styled "king of Prussia" who believes that if you break the law, at the very least you've got to be honest. But Carter's approach to tax evasion as a kind of community service that benefits everyone is threatened when the upper-class wife of a local botanist gets involved in Carter's schemes and tries to turn a profit. It's a sign of how the brilliant Kneehigh is not just a theatre company but a real theatrical family that they've managed to reassemble most of the cast of the sell-out 1996 Donmar run of Nick Darke's hugely enjoyable play to perform it in their new nomadic tent venue.
The Asylum, Tywarnhayle Farm, Sat to Thu
Lyn Gardner