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Medieval Willisau is nestled in the Lucerne landscape at the foot of the Napf Mountain. The city and its cultural offerings delight visitors with their great variety. The main lane, which is divided into sections by three heptagonal fountains, dominates the Old Town. This is also where the town hall is located, which was rebuilt in 1704 after the fourth fire. The Upper Gate was destroyed in the third city fire in 1471 and rebuilt in the 16th century. The Lower Gate was burnt to ashes several times, but was rebuilt true to the original in 1980.
The bailiff's castle, which is located above the Old Town, was built between 1690 and 1695. Remnants of the old city wall are found there. Among the other noteworthy sights are the pilgrimage chapels and the largest church of the Lucerne region.
A vast but varied hiking area offers countless possibilities. From the lowlands of the Wigger valley, the dreamy little valleys and forests to the crests of the Napf area unadulterated nature can be enjoyed. Museums, historical village centres and cultural sites make an excursion worthwhile.
In the cold season Willisau has to offer a lot as well, the seasonal fairs, the atmospheric Christmas market or the crazy carnival days. The cultural offerings in Willisau and in the whole region are especially ample in this season. The possibilities for sports activities are manifold, too, indoors and outdoors.
Calmness and recreation can be found, wandering on the foothills of the Napf, which are often free from fog in autumn and winter as well. Bikers like practising their demanding hobby in these seasons especially. Those who have achieved the ascension onto the car-free Napf, can`t expect a sweeping ski-run descent. But whoever has enjoyed the unique view from this summit on a sunny winter day, will return again and again.

subset: commoncrawl


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Serves 4 |
Prep Time 15 minutes |
Cook Time 15 minutes
1 cup Eden Arame, 1/2 package, rinsedor 1/3 package of Eden Hiziki
1 cup water, for cooking arame
1/4 cup red onion, minced
1/4 cup organic sweet corn, blanched 2 minutes and remove skins
1/2 cup Chinese cabbage, sliced and blanched 1 minute
1/4 cup broccoli florets, blanched 1 1/2 minutes
1/4 cup red radishes, chopped
1/2 pound organic extra firm tofu, drained
1/4 cup water
1 Tbsp lemon juice, freshly squeezed
1 1/2 Tbsp Eden Brown Rice Vinegar
1 tsp Eden Ume Plum Vinegar
1/4 cup fresh chives, finely choppedor scallions
1 tsp dried dillor 1 T. fresh dill, chopped
Place the arame or hiziki in 1 cup water, cover and bring to a boil. Reduce flame to low and simmer 10 minutes. Remove, rinse quickly under cold water and chop arame into 2 inch lengths. If using hiziki there is no need to chop. Discard cooking water. Place in a mixing bowl with the vegetables. Prepare the dressing by placing tofu and all other ingredients into a blender or food processor. Puree until smooth and creamy. Mix the vegetables and arame together and place in a serving bowl. Spoon tofu dressing over each serving.
Eastern, Gluten-Free, Good/High Fiber, Good/High Protein, Japanese Food, Low Fat, Macrobiotic, No Sweetener Added, Salad, Sea Vegetables, Vegan, Vinegars, Wheat-Free


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Friday, August 15, 2014
Theresa Kendall is keenly aware of the challenges presented to parents of special-needs children.
Kendall is the mother of R.J., who has autism and cognitive impairments. He gets around with the help of walking devices and a wheelchair. Simple tasks most take for granted become huge undertakings.
Her experiences while grocery shopping with R.J., who turns 8 later this month, were difficult, to say the least. She would push R.J. in his wheelchair while pulling a cart behind her, all the time making sure he didnt grab items off shelves or reach for other peoples buggies as they passed by.
I was in desperate need to see if there was anything available to be able to grocery shop with my son, she said.
Her desperation prompted her to do some research on the Internet. Thats where she discovered a site called Carolines Cart, created by another special-needs mother, Drew Ann Long, who helped develop and market a specially designed cart that allows caregivers to secure a child or adult while shopping.
I started in January of last year on this mission, Kendall said. I got in touch with Mrs. Long and she helped me get the process going.
With the help of Jim Bongalis, manager of the Goose Creek Publix store on St. James Boulevard, and Publix corporate official Jonathan Stiles, Kendall was able to get one of Carolines Carts at the store.
I approached them about the need for this type of cart, Kendall said. They told me Id have to show a need for it, so I started a campaign. I asked other families to share their own experiences or what they had observed while shopping at Publix.
Those testimonials convinced Publix officials, and the new cart was delivered in July.
This cart means so much to me and other special-needs caregivers, Kendall said. Grocery shopping might seem like a simple trip for most people, but to others its not.
I really want to thank Publix for going above and beyond for their customers needs.
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Thank you for following Powellmade. Hope to uploading more very soon.
Love the wax tutorial! I need to buy a heat gun. Thanks for posting it up.
Kind regards
Love your work. Inspiratiuon.
Thanks for following! I am a big fan of your work!
Thoughts requested :)
Thank you for following me :). You are awesome.
Yeah, I was looking into getting my survival tool design machined (I threw in my concept drawing of it) and realized how expensive it actually is, with the metal price and accounting for sharp corners and all that... but now that I know the coupon code thing, maybe I'll look into getting some fishbones... :)
Oh, yeah, and if I were to sell these would you consider it copyright infringement or whatever? Because I thought it would be cool to have a gear tie incorporated as well as a knife, flint striker, etc.
Have a nice day.


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EAST WATERFORD - Sandra K. "Sandy" (Tyson) Diven, 68, formerly of Anderson and Fountain Inn, S.C., and Middletown and Honey Grove, died Friday, Nov. 2, 2012, at her home, in East Waterford.
Born Aug. 16, 1944, in Lewistown, she was a daughter of the late Reuben and Mildred (Young) Tyson.
She is survived by: her husband of 50 years, Harvey R. Diven, whom she married on Oct. 12, 1962; one daughter, Mindy Diven Keating and husband, Edward, of Bryans Road, Md.; one son, Harvey R. Diven Jr. and wife, Keri, of Franklin, Ky.; three sisters, Linda Hause and husband, Edward, of Tower City, Betty Snyder and Sharon Kuhn, both of Loysville; one brother, Reuben Tyson, of New Cumberland; eight grandchildren, Samantha Keating Schrauder and husband, Austin, of White Plains, Md., Joshua Keating, of Bryans Road, Md., Nicole Diven Lewis and husband, Matthew, Jesse Diven, and Eliza Diven, all of Elizabethtown, Cliff Stiffey, of Hershey, Jade Miller and Meah Rigg, both of Franklin, Ky.; eight great-grandchildren, Jacob Keating, of Bryan's Road, Md., Austin Schrauder, of White Plains, Md., Gabriel and Mikayla Lewis, of Elizabethtown, Aiden Radican, of Franklin, Ky., and Faith, Christian, and Kevin Stiffey, of Hershey.
Sandy had worked in the school cafeteria and at Kauffman's Sub Shop, both in Middletown. She was a member of the Women of the Moose.
She collected angels and loved NASCAR and Penn State. She enjoyed sewing, especially making things for her grandchildren.
Her funeral will be at 8 p.m. Tuesday, Nov. 6, at Guss Funeral Home, Inc., 20 S. Third St., Mifflintown, with the Rev. Dr. Dennis R. Thompson Jr. officiating. There will be a viewing from 6 p.m. until the time of the services Tuesday, at the funeral home.
Since Sandy is being cremated after the service, the family asks that, in lieu of sending flowers, those who desire make memorial contributions to the American Diabetes Association, 3544 N Progress Ave, Suite 103, Harrisburg, PA 17110.
Condolences may be left at www.gussfh.com.


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Saturday, July 27, 2013
The Moultrie News welcomes Amanda Bush and Cierra Cathey to our multi-media consulting team.
Publisher Vickey Boyd said, "They both bring energy and enthusiasm to their jobs. Amanda has worked with newspapers before in the Summerville market. Cierra comes to us with a marketing background.
"Both have had extensive customer service experience. Both multimedia consultants are great additions to Island Publications."
Bush and Cathey have begun meeting their clients and making new connections.
For information about how our multi-media consulting team can help your business, please contact Amanda at firstname.lastname@example.com or firstname.lastname@example.com.
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Welcome to the new Brookings Public Library website! We have a new look, a new host, and new software. Along with the new layout and look, you'll find a number of new elements and pages. Check out our event calendar in the upper right corner where you can view upcoming Library events. There is also a Goodreads link on the left side where you can view new books for the month. Like the old website, you can search our online catalog (search box to the left) and go to the South Dakota Titles to Go page for e-books and audiobooks.
Also new is the Resources link at the top of the page. This gives you suggested websites to visit for fourteen different topics from Books and Readers Advisory to Languages and Style Sheets.
We'll be adding more features as time goes on. Please let us know what you think. You can do that by emailing one of us listed on the Contacts link (top of page) or by friending the Brookings Public Library on Facebook and telling us there. We're excited about the possibilities and hope you will be too.


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SYCAMORE - One mission accomplished, one more ready to begin.
Mohawk completed an undefeated run through Midland Athletic League play with a 25-5, 25-9, 25-11 victory over North Baltimore.
"We've had some very good teams over the years who didn't accomplish winning the league, so I'm very proud of this group for getting it done," Mohawk coach Eric Hoover said. "Now we can turn our focus to the tournament."
Sarah Parker, Kasey Adelsperger and Aubrie Flood all shared the team-high for Mohawk (19-2, 11-0) with eight kills. Adelsperger also had four blocks, Ashley Parker added six to go with her 22 assists.
Blue Jackets drop Stockaders
OLD FORT - New Riegel dominated Old Fort 25-10, 25-9, 25-18 Thursday in Midland Athletic League play.
Kara Scherger's 12 kills, 17 digs and five aces led the onslaught for the Blue Jackets (21-1, 10-1 MAL).
Cassandra Acree handed out 25 assists, Lauren Ladd lined 14 kills and Taylor Arbogast smacked 11 kills. Morgan Noftz added six assists and Alexis Kieffer drilled four kills.
Kristin Bender's nine kills and seven digs led Old Fort, while Sammy Miller came up with 19 digs.
Krista Ward had nine assists and six digs, Sam Hartsel lined four kills and Shelby Bilger added four digs.
Truckers flatten TC
Norwalk overpowered Columbian 25-20, 25-14, 25-17 Thursday in Northern Ohio League play.
The loss dropped the Tornadoes (5-14) to 2-8 in the league.
Megan Murphy's eight kills and 10 digs led TC, and Sarah Berecz had nine kills and five digs against the Truckers.
Tong's record not enough for Carey
CAREY - Hanna Tong eclipsed the former mark for career kills with a big night, but it wasn't enough for Carey in a 25-10, 22-25, 25-18, 25-21 loss Thursday to Fremont St. Joe.
Tong drilled 27 kills to give her 859 in her career, topping the mark of 837 set by Ashley Funk from 1994-97. She also smacked five blocks for the Blue Devils (13-8, 7-4 Midland Athletic League).
Shelby Kin crushed 11 kills and three blocks, Sydney Wentling had 39 assists and three aces, Logan Johnson added 22 digs, Makenzie Wentling came up with 13 digs and Lauren Wenner lined three blocks.
Senecas bounce back
KANSAS - Calvert apparently doesn't handle losing well. After suffering their first two Midland Atheltic League losses back-to-back, the Senecas opened up their match against Lakota by routing the first two games, then edging out the Raiders in the third for a 25-8, 25-9, 25-22 victory.
With the win Calvert (14-7, 9-2) takes third place in the Midland Athletic League.
Amber Perry led the way for Calvert with 10 kills. Meridith Lucius and Haley Felter each slammed six while Lindsay Bickley and Jordan Gase each chipped in four. Kaleigh Hendrix dished out 22 assists.
For Lakota (8-11, 5-6), Katie Park led with five kills. Stephanie Miller nearly posted a double-double with 10 assists and nine digs. Alaina Bickford led defensively with 20 digs while Khilee Baltz chipped in 11 digs to go with a pair of aces.
Chieftains gang up on SE
BASCOM - Three Hopewell-Loudon players turned in double-doubles and another nearly did so Thursday in a 25-20, 25-22, 25-17 Midland Athletic League win over Seneca East.
Courtney Burns and Shelby Sauber both hammered 11 kills, with Burns adding 22 assists and Sauber sending up 12 for the Chieftains (9-13, 6-5 MAL).
Kassie Shaver (2 aces) chipped in 10 kills and 10 digs, and Logan Frank had 10 digs and eight kills. Burns (2 solo blocks) also registered six aces and five digs.
Kassie Welly came up with 15 digs for H-L and Hannah Williams served three aces.
Mohawk turns away Bettsville
FOSTORIA - St. Wendelin dropped Midland Athletic League foe Bettsville 25-10, 25-10, 25-18 Thursday.
Allie Rutter drilled seven kills and handed out seven assists for the Mohawks (11-11, 4-7 MAL) and Meghan Reiter tossed up another 10 assists.
Colleen Fondessy and Olivia Zimmerman each belted six kills, with Fondessy adding eight digs and three blocks. Georgia Kitchen and Taylor Lucius each had eight digs and Adrienne Emerine chipped in seven.


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With his red ragtop Jeep weighted down by chain saws and other tools, Altoona chain saw artist Tim Klock was not surprised when border officers at Niagara stopped him in September to search his vehicle as he was crossing into Canada.
"I would have been surprised if they didn't search me," he said, adding that he'd traveled to Canada several times before without problems.
During the search, Klock said he was told to enter the border patrol building for a few interviews, and that he could retrieve his passport at the end. He told them he was there to visit some friends, perhaps conduct a carving seminar and enjoy a few days among fellow artists. He then asked the last officer inside where his passport was.
Article Photos
Mirror photo by J.D. Cavrich
Tim Klock, a local chain saw artist, was detained by Canadian officials for 13 days in September after a misunderstanding at the Canadian/U.S. border near Niagara.
"I don't have it. ... It's in your vehicle," said the officer, according to Klock.
He went back to his Jeep, found his passport and drove off to a friend's house.
Within 24 hours, Klock found himself behind bars.
Arrest and charges
"I don't think [Canadian Border Services Agency officers] wanted him to go into Canada," said Richard Kurland, a national immigration attorney in Vancouver. "His car was full of chain saws."
Kurland said Klock probably assumed he was allowed to enter the country because officers didn't hold onto his passport. He said no explicit statement from officers - and especially no "Welcome to Canada" greeting - means no entry for a visitor.
However, Kurland said there are procedures border officers have to follow, and leaving Klock's passport in the vehicle was wrong. He said it should have remained with the officer in charge of Klock's case and returned only once they told him he was OK to enter or had to turn back.
"I don't think it's sufficient to leave a passport on the car seat with no decision either way," he said. "I see problems on both sides of this thing."
Klock's attorney, who did not wish to be identified by name, confirmed there weren't any drugs, weapons or other contraband in the car when Klock entered Canada.
He said the main point of contention is that Klock believed he entered legally, while border services agents said he was not granted entry, but was supposed to return to the border building in Niagara for another round of questioning.
Kurland said that of the 200 million people who visit Canada each year, more pass through Niagara than anywhere else, meaning officers there deal with more illegal immigrants, drug smugglers and weapons dealers.
Tossed in jail
Klock said he was unaware of any problem until the following morning, when he and some friends gathered to discuss carving.
Before they could finish breakfast, five armed men appeared outside the house looking for Klock. He said they told him he was under arrest for running the border, leaving the port without permission and disobeying an officer.
They drove him back to Niagara to St. Catharines Jail. He said they told him he was never granted entry to Canada, but despite his protests, he was told charges had already been filed.
"I thought, 'What am I doing here?'" he said, but then theorized that it would be over soon. "I'm an American. They're our allies. There's no guns, no drugs ... "
In the courtroom
Before he entered the courtroom on day two, Klock said he was told not to speak before the tribunal. He also said his court-appointed duty counsel, who was to represent him, "couldn't have been familiar with my case. He had never talked to me."
Kurland said at that point, Klock's counsel should have discussed the other options available to him, including asking to leave the country.
"There's wiggle room," he said.
If Klock had asked to leave the country and explained his circumstances, Kurland said, the tribunal or immigration officials may have decided that it was in everyone's best interests to grant his request.
Klock said he was able to speak with a U.S. consulate official before the end of the week after his Tuesday arrest, but since no one could help him with the criminal charges, he told them not to waste resources on what he thought would be a swift resolution and end to his plight.
'Everything's steel'
Klock said that first weekend in F block, he was in a 50-by-30-foot room that was supposed to house 26 prisoners but actually housed 36. The extra men slept on 1-inch mats on the floor.
"Everything's steel and bolted to the floor," he said, including two picnic tables, two toilets, a urinal and a sink the men had to share.
Despite the grimy conditions and being punched in the face by another inmate, the worst part was being locked in a box, he said.
"I live outside. I drive a ragtop Jeep. I'm not made for this," he said.
Klock called the consulate again on the eighth day and was transferred to a two-person cell, where he was given clean clothes, blankets and towels. But with new cases given preference, his was bumped to the end of the docket.
By this point his longtime girlfriend, Tracy Reilly, and a friend had hired an attorney and arranged the $5,000 bail. But Klock still had to wait until the end of the second week to get "his day in court."
He said he was cross-examined by the prosecuting attorney and was told that because of the steps he took to secure bail and a lawyer, they believed he would work to have the case resolved.
Reilly said she found out about Klock's arrest through a Facebook message the day after.
She said she was unable to contact him, so she called the consulate and various other organizations that assist Americans who are jailed in other countries. Because Klock wasn't being mistreated, they all told her they couldn't help.
"I lost 10 pounds in 13 days," she said. "I had no control. There was nothing I could do."
Twelve days after his arrest, Klock was released and allowed to drive home. He said he was eager to fire up his chain saw to make up the money he lost from missing two lucrative fair appearances while in jail.
His friends and fellow carvers also stepped up to help.
As Klock sat in his sawdust-littered studio last week, tears welled up in his eyes. He pointed to carvings in a corner that his friends have bought, some of them at double their value; others have made sizable down payments in advance for pieces they want him to create.
"Thank God for my friends," he said. "Contributions came from all over the ... world."
So far, Klock has spent more than $8,000 and, should the case move to a full trial, he said he can expect to pay between $8,000 to $10,000 more.
Canadian Border Services Agency officials declined to comment on Klock's case because it is still open. The next hearing date is scheduled for Thursday.
Klock doesn't have to be present, but will have to return to Canada should the prosecutor decide to move to a full trial, which his attorney said is possible.
Klock said he would like to travel back to Canada some time for happier reasons but can't imagine what would happen if he is found guilty and has to return to prison.
"I'm not built for that stuff. I'm not a criminal. ... I'm just looking forward to it being over."


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These schedules are effective 4/31/14.
Metro Transit provides fixed-route public transit service to the Kalamazoo urbanized area, consisting of the cities of Kalamazoo, Portage and Parchment and the townships of Comstock, Cooper, Kalamazoo, Texas and Oshtemo.
Metro Transit routes have frequencies of 15-minutes, 30-minutes, 45-minutes, and 60 minutes, depending on the time of day and the route. Listed below are the routes and their respective departures from downtown during peak hours (Monday - Friday 10 am - 3 pm and after 7:15 pm), non-peak hours, and Saturdays.
Metro Transit operates Monday - Saturday from 6 am to 10:15 pm. No Sunday service is provided.
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Serving Hardings on Howard, Meijer on Westnedge, and Crossroads Mall
Serving Milwood Elementary, Heritage Hills Apartments, Milwood Apartments, Washington Elementary, Washington Square Apartments, and Devonshire Apartments
Serving Western Michigan University's main campus at the Rood Hall Parking Lot, Lot #61, The Arboretum, Concord Apartments, Hardings on Drake, Westland Meadows, Village Apartments, West Main Mall, and Maple Hill Mall
Serving Parkview Hills Apartments, Evergreen South, Rosewood Complex, Parkview Hills, and Springmont and Madison
Serving East Main Street and Eastwood Plaza
Serving the City of Parchment, Barclay Hills, Parchment High School and Walmart on Gull and G Ave.
Serving the Metro Transit offices, Fox Ridge Apartments, Northwind Apartments, Hillside Middle School, Maple Hill Mall, and Kalamazoo Central High School
Serving Cork & Fulford, Wings Stadium, Milwood Junior High, MRC, Washington Square Apartments, and the Industrial Park on Covington
Serving Borgess Medical Center, New Horizon Village, Nazareth Center Complex, Meijer and Walmart on Gull Road
Serving Mayors' Riverfront Park, Kalamazoo County Fairgrounds, Kalamazoo County Sheriff's Department, Kalamazoo County Jail, Comstock Adult Ed, and Meijer on Gull Road.
Serving Kalamazoo Valley Community College - Oshtemo Township Campus, Western Michigan University, and Evergreen South Apartments
Serving the YMCA, South Middle School, St. Monica's School, Hackett High School, Meijer on Westnedge, Candlewyck Apartments, Milham Park, Loy Norrix High School, and the Kalamazoo-Battle Creek International Airport
Serving Bronson Hospital, Sky Rise Apartments, Pathways, Michigan Works, Hilltop Apartments, Candlewyck Apartments, Meijer on Westnedge, and Goodwill Industries
Serving Maple Hill Mall, Meijer on West Main, Friendship Village, and Evergreen North Apartments
Serving the Metro Transit office, Douglas Community Center, Interfaith Homes, Lincoln School, North Christian School, and the Northside Prep School
Serving Kalamazoo City hall, Oak and Lovell, 58 West Apartments, and Western Michigan University's main campus at the Rood Hall Parking Lot, Lot #61
Route #21 - Solon Kendall Lafayette
Serving Western Michigan University's main campus at the Rood Hall Parking Lot, Lot #61, Kendall Manor, Whitehall Apartments, and the Landing Apartments, Fraternity Village, Greenwood, and Knollwood Apartments when WMU is in session during Fall/Spring Semesters.
Serving the Rosewood Complex, Anna's Vineyard, Milham Meadows, Crossroads Mall, Romence and Oakland, and Borgess at Woodbridge
Serving Crossroads Mall, Portage & Romence, Portage Industrial Park, Milham & Meredith, Portage City Hall, Walmart, and Meijer on Shaver


text:
Source: CNN
quote: confusing the marketplace between what's an ultrabook and what is an ultrathin notebook.
quote: Your smartphone and tablets do not have them either, noob.
quote: About a month ago, Intel cut the outlook for its Q3 earnings by $1.1 billion. The reduction in earnings indicated that perhaps Intel's ultrabooks weren't selling as well as the company wanted.


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Wall Street Decline Takes Its Toll...
Expanding Use of NGS Has Largely...
LC-MS Gains Favor Due To the...
As Immunotherapy Revolutionizes the Cancer Treatment...
The determination of complete genome sequences for a wide variety of experimental organisms established a first step in the search for an in-depth understanding of the complex genetic functions that define living entities. Extensive genetic, biochemical, cytological, and physiological analyses are now required to correlate genome sequence with the next level of understanding of genetic function.
As such, technologies that allow researchers to routinely and efficiently edit virtually any genome—by directing mutations in a truly targeted manner—will greatly enhance the understanding of basic biology and lead to novel ways of treating human disease.
Early approaches to genetic manipulation of mammalian cells used ionizing radiation or chemical-induced mutagenesis to make alterations to the genome. But the random nature of these nutations represented a major limitation to studying gene function. More recent methods have enabled precisely targeted genomic editing—driven by homologous recombination (HR) between an exogenously supplied donor DNA and the specified target locus. The low efficiency of this approach, however, has necessitated sophisticated positive and negative selection strategies in order to isolate the rare targeted events.
In the early 1990s, the discovery and manipulation of zinc finger protein domains started a field of research that enabled an alternative targeted approach to genetic manipulation in higher-level eukaryotes that was over 1,000 times more efficient than the prevailing HR-based gene-targeting methodologies.
Zinc fingers are structures of about 30 amino acids held together by a zinc ion, and they determine the DNA binding specificity of the most abundant class of transcription factors found in a wide range of species. Each zinc finger—which binds to a triplet of base pairs in the target DNA—can be engineered to exhibit selectively for a specific triplet. Multiple zinc fingers can be linked to generate a larger zinc finger protein (ZFP) that recognizes a DNA sequence that is a composite of the individual finger target triplets. For instance, four zinc fingers can be assembled into a ZFP that selectively recognizes a specific 12 base pair target.
Also of critical importance was the discovery that a zinc finger DNA-binding domain can be linked to various catalytic functional domains and so target those functions to a specified endogenous locus. Thus, by linking to a ZFP to the catalytic domain of the endonuclease FokI it is possible to create a targeted zinc finger nuclease (ZFN).
But the endonuclease subunit of FokI must dimerize in order to cleave DNA. Therefore, ZFNs are designed as a pair, to bind to adjacent sequences in the target DNA with precise sequence specificity and spacing—in essence creating a pair of very specific genomic scissors (Figure 1). Delivery to the cell of plasmids or mRNA encoding the ZFNs leads to transient expression of the nucleases and subsequent generation of a double-strand DNA break (DSB).
The power of a ZFN-induced DSB comes from the fact that a DSB triggers either of two DNA repair processes— non-homologous end joining (NHEJ) or homology-dependent repair (HDR)—that can be harnessed to evoke desired genomic alterations. The error-prone repair mechanism of NHEJ often results in localized mutations due to deletion and/or insertion of short sequences at the DSB site. The occurence of such mutations within a coding sequence typically results in disruption of functional gene expression. Thus, this pathway has proven most useful for rapidly achieving targeted gene knockout.
Alternatively, in the event that the desired outcome is insertion of a transgene into a user-specified site in the genome, a donor DNA construct can be codelivered with the ZFNs. The donor contains the transgene of interest flanked by sequences homologous to either side of the ZFN cleavage site. In the presence of this donor, the DSB is repaired via the HDR pathway, by which the transgene is effectively copied into the endogenous target site (Figure 2).
ZFN-mediated gene targeting is a powerful and versatile tool for targeted genome editing of a wide range of organisms and cells. The process of ZFN-mediated gene modification is as simple as a single transfection experiment and edits occur in as little as three days. With as much as 20% of the cells undergoing targeted genomic editing, selection markers are most often unnecessary. Once high-quality ZFNs have been constructed, isogenic stable cell lines containing the modifications can be generated in as little as a month through simple dilution cloning of ZFN-treated cell pools.
Gene knockout—the complete disruption of gene function—is the most powerful tool for determining gene function or for permanently modifying the phenotype of a cell. In addition, gene knockouts can be created in multiple cell lines in order to identify and/or validate a potential drug target and provide a way to completely delete the functions of genes that are not amenable to technologies such as RNAi.
ZFNs offer a rapid single-step approach to targeted gene knockout in mammalian cells. As an example, ZFNs have been used to target the dihydrofolate reductase (DHFR) gene in a CHO cell line diploid for a functional DHFR gene. In this particular study, gene disruption at a frequency of >1% was obtained via the NHEJ pathway without the use of selection markers, and studies were done to confirm that deletion of the gene at the DNA level did indeed translate into a phenotype expected of a DHFR-null cell line.
ZFN-mediated gene integration allows placement of exogenous genetic sequences precisely into user-specified sites in the genome with high-efficiency. This newfound ability will not only lead to a new generation of functional genomics studies, but it will have great impact in the drug discovery world as it will allow the creation of new cell lines containing fusion tags or reporters that can be used for more efficient cell-based screening.
In order to gain acceptance as a robust methodology, any means of targeted gene integration should occur at a frequency high enough to obviate the need for time-consuming selection of cells carrying the desired gene.
Indeed, using the ZFN methodology, targeted gene-integration frequencies of 15% and 6% have been observed, respectively, for insertion of a 12 bp tag and an 8 kb antibody expression cassette at a specific location in human cells, without using selection for desired recombinants.
In 2007, Sigma-Aldrich formed a partnership with Sangamo Biosciences to make ZFN technology available to research scientists through its CompoZr™ line of products and services.
The CompoZr custom ZFN offering, launched recently, supplies partners with zinc finger nucleases that have been validated to target and edit customer-defined genes of interest.
Future CompoZr products based on ZFN technology, available in 2009, will include ZFNs targeting popular genes, gene families, and pathways.
The ability to routinely edit genes in cell lines, animals, and plants has long been a goal sought after by researchers seeking an in-depth understanding of complex genetic functions and applying that knowledge to develop new pharmaceuticals, improve agricultural productivity, and even cure human disease through gene therapy.
ZFN technology promises to realize this goal by providing the tools to precisely and efficiently induce genetic changes in systems ranging from human stem cell lines to fruit flies, plants, and a host of other organisms.
Greg Davis, Ph.D., is R&D scientist, Trevor Collingwood, Ph.D., is strategic development manager, and Phil Simmons ([email protected]
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Nice to meet you. We're poppin. For those who care about these kinds of things, our full corporate name is poppin inc., and we are a delaware corporation.
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This is getting long! We know. Our lawyers made us do it.
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Please send any questions or comments (including all inquiries unrelated to copyright infringement) regarding this Site to:
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Developers of DTC Genetic Testing Are...
Expanding Use of NGS Has Largely...
NSCLC is "Major Battlefield" as Merck,...
LC-MS Gains Favor Due To the...
Single-cell analysis is becoming increasingly important for applications in cancer, stem cell development, and cytotoxicity. Localizing individual cells at defined locations is a critical step in the single-cell analysis process. While it is now possible to fabricate well-defined single-cell patterns using arrays of cell-adhesion proteins, the understanding and control of surface properties, pattern geometry, and assay conditions are crucial for optimal single-cell array construction. Here are seven tips for successfully patterning single cells on cell-adhesive protein arrays.
1. Choose an appropriate surface and the best surface chemistry for attaching cell-adhesive proteins. A functional surface (such as epoxy, aldehyde, or Succinimide) chemically couples the adhesive protein to the substrate, which is critical to withstanding subsequent washing steps and potential surface protein delamination.
2. Use the ideal cell-adhesive protein for the cell to be arrayed. Most cell types are capable of binding to certain specific proteins, but not all cells attach to the various extracellular matrix (ECM) proteins with similar efficiencies. As an example, fibroblasts bind well to fibronectin.
3. Tailor the size and shape of the cell-adhesive protein pattern to the size and shape of the cell being attached to it. Large cells will have difficulty binding to very small protein patterns, while multiple small cells (and not the desired individual cell) will potentially attach to larger patterns; neither outcome is desirable. Depositing cell-adhesive protein features in pattern shapes that are conducive to cell growth will also contribute to successful single-cell arrays. For example, when arraying neural-like cells, long lines of cell-adhesive protein features are most suitable. When creating cardiac myocyte single-cell arrays, rectangular box patterns of cell-adhesive proteins will enable cells to bind in the rod-like shape they take on in vivo.
4. For the most robust single-cell cultures, work with optimal cell numbers and feature sizes. Using too few cells for attachment to arrayed cell-adhesive proteins will result in insufficient pattern coverage. Large protein features can accommodate lower cell densities (number of cells per unit area) to achieve single-cell array conditions, but too low of a cell density often results in poor coverage of the patterned features. To most efficiently cover protein patterns with predominantly single cells, small features of cell-adhesive proteins and high cell densities should be used.
5. Select the blocking agent that best minimizes nonspecific binding for the specific study being undertaken. As an example, experiments that involve cell patterning followed by motility assessment will work best with a blocking agent such as bovine serum albumin that facilitates specific cell deposition and cellular migration away from patterned areas. Alternatively, in studies where placing the cells and preventing cell motility is important, engineered polymers with up-facing polyethylene glycol groups might be the best blocking agents. In general, hydrophobic blocking agents are not conducive to cell attachment, cell spreading, or cell motility, while hydrophilic blocking agents promote more cell attachment and cell movement over time.
6. Employ appropriate incubation conditions, especially time and media. An experiment's particular cell-adhesive protein binding dynamics and blocking conditions must be taken into account to determine optimal cell plating time. If cells bind quickly to a specific cell-adhesive protein, incubation time can be short; if cells bind slowly, longer incubation times are necessary. A balance is needed; allow ample time for cells to bind to cell adhesive proteins but not enough time to permit cells to bind in nonspecific locations. Plating media will have a big impact on cell binding speed. Although serum in the media generally contributes to faster attachment of cells to arrayed proteins, serum can coat the nonpatterned surface and lead to high nonspecific binding. In general, serum-free and low-serum plating media generate the highest success rates when plating adherent cells. After cell patterning, for longer term studies, complete media can be used successfully.
7. Use proper washing procedures. After the initial binding of cells to the arrayed cell-adhesive proteins, adequate washing is critical to the creation of desired single-cell patterns. Wash conditions will be dependent on the binding strength between the cells and the patterned adhesive proteins. Use standard manual pipettes (capable of delivering volumes compatible with the size of the substrate) and customary wash reagents; 1 mL volumes of warm PBS typically work well. Employ a moderate wash flow rate. Strong washing (using a vacuum aspirator or flowing wash solution directly over the top of the patterned cells) will likely remove nonspecifically bound cells but may also separate some cells from their cell-adhesive binding proteins. A less-intensive washing technique (i.e., adding wash solution to the culture dish wall) may leave many cells that can subsequently bind nonspecifically. In general, multiple, gentle washings will allow for optimal coverage of protein patterns without separation of cells from patterned protein.
John Collins, Ph.D., is senior applications scientist at NanoInk.
John Collins, Ph.D., is senior applications scientist at NanoInk.
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I hope I don't get a lot of negs. I was not calling it a bad signing, or that Helm is a bum and not worth it or that Holland should be fired. I was just hoping for less of a cap hit in order to make sure we have the cash to bid high of the players we need to change this team. I am glad Helm is going to be part of that and hope that he signs again in 4 years to a lifetime contract...I just hope that this trend doesn't continue with the overpayment (around the league) because like I said, it's going to ruin the NHL when the Colton Orr's are making $4M soon...
That being said, we still have $24M left to spend of Abby, Parise, Suter, Harding & Schultz & Dekeyser & Welsh...so far I haven't lost out on anyone from my wish list! (yet!)
Every little bit of cap space is going to count. You over pay a bit here n there, and you go from having a solid back up goalie to being stuck w a conklin again.
I do think Helm is worth every penny though. It is hard to find players that play as hard as he does every game. Having those types of players makes everyone (except Franzen i guess) want to play that much harder.
Its a good start Kenny. Keep up the good signings.


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text: I am wondering if i am alone out here. As anyone else with Rheumatoid Arthritis I have a laundry list of ailments and medications all managed by my fabulous Rheumatologist...*insert thank you note here** I have gone through some phases so far of this disease: Denial (which was silly and quickly had 3 Dr.s all agreeing that I need to start RA meds), Confusion, resolved with Education, Depression (caused by the Education), now I am nearing Acceptance. Anyone else out there? I am only 27.. just doesn't seem fair. I know neither is life, however, why is it that no one understands my constant pain? Why do people think i am in a bad mood when i forget to wear my mask? its not that i promise it's only the 10 new nodules on my hands, the redness, the fevers, sleepless nights, the swelling, the embarrassment of not being able to move my hands or feet or shoulder or neck! Cant they see I tried to put on my mask today, its just that my hands stopped working half way to the finish line! Please if I am not alone here I'd love to hear your story of your mask that you put on everyday.


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Expand hobby horizons with aquatic possibilities in miniature. Fluval SPEC Desktop Aquarium Kit is an elegant nano aquarium that's big on style and high-end features. Advanced LED (light emitting diode) lighting brilliantly illuminates aquarium setup with minimum energy use and heat output. Integrated 3-stage filtration is discretely concealed behind the back wall for attractive presentation. Stylish aquarium design, powerful filtration and brilliant illumination set the stage for creative stocking and aquascape options.
2 gallon Fluval SPEC Desktop Aquarium Kit boasts quiet operation thanks to its built-in filtration system. A 40 gph circulation pump with adjustable flow rate is at the heart of this unique 3-stage filter. A large Foam Filter Block traps particles and debris for exceptional mechanical filtration. Innovative media niches secure Activated Carbon Insert and BIOMAX Insert in the Foam Filter Block for space-saving, layered chemical and biological filtration. This over-sized filtration system streamlines maintenance and creates a clean, clear and healthy environment for aquarium fish.
Aquarium decoration and aquarium substrate, sold separately.
Shop all Fish Aquariums.
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Scott Moirs uncle Paul skates to the rescue of bladeless Belarussians
By Ryan Pyette, The London Free Press
Thursday, March 14, 2013 10:41:37 EDT PM
After a nightmare trip, Belarusian ice dancers Viktoria Kavaliova and Yurii Bieliaiev finally arrived at their first senior worlds in London on Wednesday morning.
Their skates didnt.
Enter Ildertons Paul Moir, worlds volunteer, skate sharpener extraordinaire, and uncle of Olympic ice dance champion Scott. ...
Bieliaiev is 6-foot-5. He takes a size 14. We had a skater whos doing on-ice security volunteering for us this week (Chatham native and former pairs skater Adam Johnson) and he took off his skates for the boy to try on, Moir said. Theyre not dance blades but they fit him. They just had to be sharpened up.
Big Bieliaiev hasnt used that type of skates since he was five or six.
Still, it was a stroke of luck. Finding skates for Kavaliova, who takes size six-and-a-half, required a little more work. ...
Sheri, another one of Moirs daughters and a former senior-level ice dancer, offered up the skates she used when she was part of the Nexxice synchronized team powerhouse.


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Top Definition
1. Harry Redknapp. 2. Daniel Levy. 3. One who advances one's own interests by canny, aggressive, or unscrupulous behavior.
Did you notice that selfish hypocrite Wheeler Dealer tap up that poor player and denying it in the same sentence?!
TruthhhHurts tərəfindən 02 Sentyabr 2010
Trending Now
Can you define these popular missing words?
A person who like to pole dance in their moving car whilst window shopping for thongs. Enjoys eating 20 McChickin nuggets and karate chopping several asses.
- Wow did you see that machine move?
- Yeah it was such a wheeler dealer!
- Yeah it was such a wheeler dealer!
claireisaledgend tərəfindən 26 May 2009
A shrewd operator especially in business.
Many years ago my Grandmother told me while we were talking investing and money: " You will do well- You are a Wheeler Dealer". I do believe that what she meant by that is:
ohyaitsallgood tərəfindən 03 Dekabr 2015
A paraplegic drug dealer
I'm gonna go get some weed from my wheeler dealer
NotRoseMcGowan tərəfindən 23 Aprel 2015


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on December 3, 2012 - 8:54 PM
LOCKPORT – William G. Andrakulic Jr., who has been arrested 11 times for alcohol-related driving charges, on Monday blamed his use of the sleeping pill Ambien for his recent arrests.
Niagara County Judge Sara Sheldon Farkas said she didn't think that was why Andrakulic, who said he didn't drink between 1984 and 2001, was arrested in 2001, 2005, 2007 and Jan. 30 of this year.
She sentenced Andrakulic to six months in jail to start a five-year probation stint and added fines and fees totaling $5,520.
Andrakulic, 58, of Elderberry Drive, Niagara Falls, was arrested on a felony DWI charge after refusing the breath test following a collision on Pine Avenue in that city.


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LORAIN - The Beavers got a late Christmas present on Saturday.
Beaver?Local earned a first-place finish in the two-day Lorain Nationwide Holiday Classic.
The Beavers started the day in second place, but overtook Norwalk St. Paul to claim the title.
Article Photos
Beaver Local's Josh Henderson earned his 100th career victory on Saturday in Lorain en route to a 113-pound title. (Submitted photo)
Beaver Local was first with 243.5 points, followed by Norwalk St. Paul (225.5) and Westlake in the 15-team field.
The Beavers crowned three champions.
Josh Henderson won the 113-pound title with a pin of Norwalk St. Paul's Joe Livengood in 1:35. Henderson, now 18-0, also picked up his 100th career win.
At 132 pounds, Ron Foster (17-1) took first place with an 8-5 decision over Clearview's Michael Hollingsworth.
In the heavyweight division, Cody Martsolf was tops after defeating Copley's Chris Burke, 8-4.
Morley Marsden came one win away from a 160-pound title, falling by pin to Twinsburg's Alex Keller in 5:44.
Also placing for Beaver Local was:?Jack Dawson (5th, 106), Dustin Martsolf (4th, 120), Clayton Davis (6th, 126), Daniel Hasson (4th, 138), Josh Barber (5th, 145), Dan Lattanzio (4th, 170) and Cody Green (5th, 220).
The Beavers will be back in action on Jan. 3 at East Liverpool.
Team Standings
Beaver Local 243.5
St. Paul, Norwalk 225.5
Westlake 186.5
Bay 169.0
Lorain 164.5
Worthington Kilbourn 149.5
Jefferson Area 133.0
Copley 128.0
Twinsburg 124.0
Clearview 88.0
Kirtland 85.0
Midview 75.0
Holy Name 58.0
Avon Lake 51.0
Lakewood 39.0


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Some writers have so exaggerated their treatments of the good-ole-boy political and economic network that a caricature of it has supplanted reality in the public mind.
The same holds for the newsroom.
Jon Buchan, a lawyer and former journalist who has followed these arenas for more than 30 years while also defending the media in court, determined that the characters in his first novel Code of the Forest (Joggling Board Press, $24.99) would be grounded in the real world.
Set in 1995 in Georgetown, the novel channels decades of the authors experiences and observations. Buchan harbored the germ of an idea of a book for some time, but dismissed the notion of a memoir or nonfiction treatment.
Instead, he opted to take a page from what Tom Wolfe once called the New Journalism, albeit it in reverse: using the reporters eye for setting, ear for dialogue and a nose for significant detail to inform a novel.
I wanted to capture a world with imagination, showing the kinds of characters that really are out there, says the Mullins native, long a Charlotte resident. Fiction enables you to capture the inner thought processes and emotions that reveal who characters really are and chart how they develop.
Quid pro quoFor years, Buchan watched as those in political power granted or asked for favors.
Its everywhere, not just in politics, and most of the time thats pretty innocent, he says. But on another level that back scratching progresses to something less legitimate. Sometimes that culture of favors reaches out too far and becomes cronyism and the feathering of nests.
The book is about the bad old culture and the bad old South.
And it has not gone unnoticed by others fighting for the publics right to know.
Jon Buchan knows the law. He knows journalism. He knows politics, says John Drescher, executive editor of the Raleigh News and Observer. If you want to know how the South works, this novel is a good place to start.
Once bittenDuring summers between coursework at Princeton University, Buchan returned to the Carolinas to intern at The Raleigh Times. The bug bit, hard.
After college, he started his own newspaper in Columbia an alternative weekly called The Osceola with a specific intent in mind.
Its whole reason for being was to cover South Carolina politics, to discover who had power and how it was used, Buchan recalls. We started it in the early part of 1973 and I think we were making a difference.
We would get grudging praise from an editor at The (Columbia) State, whod congratulate us on a story then say they will follow up on it themselves. They liked the kind of writing Id been doing, not just straight news but offering an historical perspective and human color.
His three years in journalism half at the helm of the Osceola were about as much fun as I ever had, Buchan says. To be that young and to write about what happens over there in the Legislature was exciting.
Sea changeBut talking to legislators who suggested he was too green to grasp the details prompted a change of course.
I had been thinking about law school for some time, recalls Buchan, who earned an undergraduate degree in American history. And I was getting a little tired of hearing that because I was not a lawyer, I could not understand this legislation. So I entered Duke University Law School and earned a law degree.
Buchan assumed hed return to his first love, journalism, with his new degree another weapon in his arsenal. But serendipity took a hand when he was offered a post with a Charlotte law firm that, among other things, represented The Charlotte Observer. Buchan never looked back.
Im a big believer in the critical role of newspapers in a democracy, and at least 50 percent of what Ive done over the years is represent newspapers in all manner of disputes.
Not long after The New York Times v. Sullivan case changed libel law and gave papers more First Amendment protection, I got to handle some really interesting defamation cases.
Between the Supreme Court ruling in 1980 that the public had a First Amendment right of access to courts and 1990, Buchan says good case law was made on the importance of courtrooms being open.
It was also a great era for reporters who were being subpoenaed. We fought those subpoenas and also made some really good case law in that regard.
The playersBuchan, who has witnessed many a prickly relationship between journalists and lawyers, was keen to create characters that were believable for Code of Forest.
Wade McNabb, publisher of the (fictional) Georgetown Pilot, is bent on exposing high-stakes political corruption. And he finds an ally in Kate Stewart, a young lawyer who wants to champion David after years in corporate law defending Goliath.
Opposed is powerful Sen. Buck Ravenel, to some extent the villain of the piece. But in Buchans hands hes by no means a conventional corrupt politician, but a man of shadings whose motives are varied.
The novel employs broad brushstrokes in depicting the good-ole-boy networks and political shenanigans, but does not preclude humor or more basic interpersonal drama, says Buchan.
It is also a story of two people who suffered losses early in life and who toughen up as time goes on. Its a story with layers.
Reach Bill Thompson at 937-5707.


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MARTINSBURG - A judge's ruling allowing the Jefferson County Sheriff's Department to seize a woman's home because she sold less than five grams of crack cocaine to a confidential informant has been reversed by the State Supreme Court.
The majority opinion filed Friday reverses an order filed by West Virginia 23rd Judicial Circuit Judge David Sanders granting a summary judgment against Tricia Dean, of Kearneysville, and remands the cause back to circuit court.
Justice Margaret L. Workman delivered the opinion of the court while Chief Justice Menis E. Ketchum filed a concurring opinion. The court's majority opinion raised concerns over whether Dean's rights were violated under the constitution's Eight Amendment barring excessive fines.
"The absence of crucial evidence including the amount of the forfeiture sought by the State necessitates a remand of the case to the circuit court so that an evidentiary hearing can be conducted and a determination made regarding whether the forfeiture of the Petitioner's property is grossly disproportionate to the gravity of a defendant's offense," Workman's opinion reads.
The case came before the court after an appeal was filed in January on Dean's behalf by Shepherdstown attorney Ruth A. McQuade. Dean appealed an order signed by Sanders granting a state's motion for summary judgment to have Dean's home, located on White Tail Lane, be forfeited to the Jefferson County Sheriff's Department.
On Feb. 25, 2010, a confidential informant working with the Jefferson County Sheriff's Department made a monitored phone call to Dean and arranged to purchase $700 worth of crack cocaine. Dean told the informant that she had to wait until her drug supplier arrived at her residence before she could complete the deal.
Later that same day, the confidential informant went to Dean's home and purchased $500 worth of crack cocaine from Dean's supplier. Following the drug deal, Dean received $100 from the informant for setting up the drug deal.
As a result of the criminal investigation, Dean and others were arrested at her home. The record indicates that a criminal complaint was filed against Dean in state court on Feb. 26, 2010. That case was later dismissed due to the prosecution of Dean in federal court.
On May 19, 2010, a federal grand jury returned a five-count drug indictment against Dean. She subsequently entered into a plea agreement with the U.S. government, pleading guilty in July 2010 to one count of her federal indictment that charged her with aiding and abetting the distribution of 4.8 grams of crack cocaine in exchange for $600.
On Jan. 13, 2011, Sanders granted a state's motion for summary judgment against Dean in forfeiture proceedings. On Jan. 25, 2011, Dean filed a motion for reconsideration of the summary judgment or, alternatively, a motion to modify the order. That motion was denied by the circuit court. According to court records, the state failed to introduce any evidence before the circuit court to establish any connection between the single drug transaction Dean pleaded guilty to and her property.
"The circuit court's findings regarding the drug transaction occurring at the Petitioner's residence are not supported by any evidence introduced either as part of the State's summary judgment motion or at the hearing before the circuit court," Workman's opinion reads.
The Supreme Court found that the lower court judge erred in granting summary judgment in favor of the state. Another issue brought up on appeal before the Supreme Court was whether the circuit court erred in determining the forfeiture of Dean's property didn't violate the excessive fines clause of both the West Virginia and U.S. constitutions.
The U.S. Supreme Court has determined that some portions of federal asset forfeiture legislation are partially punitive and therefore subject to Eighth Amendment restrictions. Moreover, in Dean's case, the state conceded that the forfeiture action against her property was intended to punish and was not remedial.
"The circuit court erred in its determination that the forfeiture did not violate the Excessive Fines Clause because that determination was based solely on the culpability of the Petitioner," Workman's opinion reads.
The maximum fine Dean faced for the underlying offense in state court was $25,000. The state, however, maintained that under the federal criminal statute, Dean faced a possible maximum fine of $1 million for the federal charge she pleaded guilty to.
"We disagree with the State's position. Because the forfeiture action was initiated under state law, the authorized penalty by which the amount of the forfeiture must be compared with is the penalty to which the Petitioner is subject to under West Virginia law, not federal law," Workman's opinion reads.
There is no evidence in the court's record that supports any value of Dean's home. Likewise, the record is devoid of any evidence demonstrating any criminal history of Dean.
"There was also no evidence that the relevant property was being used for repeated drug activities or drug buys as the sole basis for the forfeiture was the single sale of 4.8 grams of crack cocaine for a total of $600. It is the lack of evidence submitted below that necessitates a remand to the circuit court," Workman's opinion reads.
- Staff writer Edward Marshall can be reached at 304-263-8931, ext. 182, or email@example.com.


text: Most campuses provide opportunities to duplicate Renaissance spaces for performance uses. The resemblance of the Berkeley campus to French Renaissance building results from its design originating from Emile Bénard of Paris with an elaborate plan in the formal Beaux Arts neoclassical style. Photograph © 2003 by Alan Nyiri. No restriction is applied to the use of this photograph in University of California publications or Web sites.


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Top Definition
When you're at your straight friend's house pounding back a few beers and your friend gets up to go to the bathroom. You take your friend's nonempty beer bottle and forcibly insert it into your anus. Using your now gaping asshole, you rub your anus all over his couch cushions, pranking your friend.
Lance got up to use the toilet so i bottleanus'd his couch
作者 Alec J Biello 2011年8月26日
Trending Now
Can you define these popular missing words?


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HOLLIDAYSBURG - A Blair County woman has become the third person to enter a guilty plea to neglect charges filed in connection with the care of an 80-year-old resident at an Altoona personal care home.
Diana Frye, 62, 1104 14th Ave., a former aide at Warner's Home for the Aged, entered the plea Friday at the Blair County Courthouse and is scheduled to be sentenced June 7 if a presentence investigation is finished.
The state Attorney General's Office, which is prosecuting the case, said Frye faces a maximum of five years in prison and a $10,000 fine.
"This case, as every abuse case upon our older citizens, is appalling," state Attorney General Kathleen G. Kane said Friday in a statement issued by her office. "The victim trusted Ms. Frye to fulfill her obligations as an aide and a human being. She failed him."
Frye's attorney, Assistant Public Defender Joseph Hartye, did not return a phone call seeking comment.
In September 2012, Frye was one of three charged with neglecting care of a dependent person after a state grand jury investigation into Kenneth G. McGuire's condition.
McGuire died Nov. 17, 2011, 12 days after being admitted to Altoona Regional with multiple bedsores, a smell of feces and urine and other hygiene-related medical issues, court documents state.
The others charged were Sherry Jo Warner, 65, 2030 Riggles Gap Road, who owned and operated the personal care home until its operating license was revoked by the state in December 2011, and Marjory J. Koch, 44, 211 Seventh Ave., who also worked at the home as an aide.
Online court records indicate that Warner is scheduled for sentencing on April 19 and Koch is scheduled for sentencing on April 25.
In the grand jury's presentment, Frye is accused of failing to tend to McGuire's care while working the overnight shift. She told the grand jury that during the early morning hours of Nov. 5, she looked into McGuire's room during her work shift, but she did not physically check his condition or the condition of his bed. That was the morning when McGuire was found on the floor between his soiled bed and dresser, partially on top of his oxygen tank, with a cut on his elbow. An ambulance was summoned to take him to the hospital for care.
Kane, in the statement issued by her office, pledged pursuit of such cases.
"We will continue to seek justice for any care-dependent person who is abused and neglected," she said.


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"We're doing okay," Spelling, 41, told PEOPLE Tuesday at The MOMS Denise Albert and Melissa Gerstein's screening of her new show, Mystery Girls. "We're working through it. It's something that's going to take time to get through."
In December, it was revealed that McDermott, 47, had been caught cheating. In the ensuing months, he entered rehab, where he was treated for an addiction to sex, and began couple's therapy with his wife, a process that was documented for their TV series, True Tori, this spring.
But all along, Spelling held out hope that they'd be able to work through their issues, sources told PEOPLE.
"Tori doesn't want to raise four kids by herself or for them to grow up with divorced parents," a friend of Spelling's told PEOPLE earlier this year. "She wants to be married to Dean, and she wants him to never have cheated on her. But of course, that's not the case."
Although it may take time before Spelling and McDermott's relationship is back to what it was, it's clear Spelling is not giving up on her husband and is willing to work through their issues and rebuild their family.
"Life is always a work in progress and our relationship is one as well," Spelling said on Good Morning America Wednesday morning. "We're moving in the right direction."


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New Scooby-Doo cereal is terrible.
Just tried the new Scooby-Doo cereal and it is terrible. It has absolutely no flavor whatsoever. It really needs something added to give it a better taste. It kind of just tastes like cardboard, to be honest. Needless to say, I will not be buying it again.
This topic is no longer open for comments or replies.
EMPLOYEE0Jessica (Official Rep) January 07, 2013 13:27Jason- Thank you for your feedback, we encourage it to best understand our consumers wants. The benefit of this cereal is that it's WIC eligible and boosts several vitamins and minerals, is low in fat, is a good source of fiber and vitamin D and an excellent source of iron. Nevertheless, we will direct your feedback to the appropriate team. Thank you again for participating in our Kellogg's® online community.
Scooby doo cereal is so bad my grandson took one bites and said throw it in the trash,a waste of 3 dollars. I agree
I disagree with what they are saying. We just bought this cereal and i tried it and for a cereal high in vitamins and minerals, and not sugary crap like lots of other cereal, it was actually quite good!
Mmmm, cardboard.
Worst cereal ever. It will not be carried by my grocery store or any I can tell about this product. Healthy does not mean taste is horrible! Kellogg's used to mean quality.
see the change log
I finally remembered to say the exact same thing, THIS GARBAGE HAS NO TASTE WHATSOEVER!!!!!!!!
I looked all over the place for this crud and it has NO TASTE WHATSOEVER!!!
In fact I'd like a coupon for some other cereal like that Chocolate Special K, you know, something with some TASTE TO IT!@
- view 2 more comments
I review a lot of cereals for my site. I had bought the Scooby Doo cereal along with a few others, and kept putting it off since it didn't sound that good, but I wound up being surprised that I liked it..
I almost get why people say it doesn't have a strong taste, since it's not an especially sweet cereal. But it does have flavor. I was very reminded of Rice Krispies Treats cereal, just much less sweet. It has that same vanilla / marshmallow flavor, but it's more of a hint of it.
Steve (Official Rep) March 11, 2013 13:23Thanks for the review, Robb! While the flavor may be more subtle than some might have expected, the cereal's profile is strong. As Jessica noted above, the cereal is low in fat, a good source of fiber, a good source of vitamin D and an excellent source of iron.
Thanks again for the feedback – we'll look forward to more of your reviews in the future.