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Eyeglasses, Sunglasses, and Magnifiers Center - Grand Island, NE
- WebMD Physician Directory of Grand Island Eye Doctors
- Related Eyeglasses, Sunglasses, and Magnifiers Articles
- Grand Island Emergency Contacts
- Nearby Grand Island Hospitals
- Find a Local Pharmacy on RxList - Grand Island
Grand Island Eye Doctors for Eyeglasses, Sunglasses, and Magnifiers
Type of Physician: Eye Doctor
What is a Eye Doctor? A certification by the Board of Ophthalmology; practitioners provide comprehensive eye and vision care. They are trained to diagnose, monitor and medically or surgically treat all eyelid and orbital problems affecting the eye and visual pathways, and to diagnose, monitor and treat all eye and visual disorders. They often prescribe vision services (glasses and contact lenses) as well as serve as a consultant to physicians and other professionals.
Specialty: Ophthalmology
Common Name: Eye Doctor
Eye Doctors in Grand Island * results
- 2
Robert S. Proffitt
Proffitt Eye Center
711 N Custer Ave
Grand Island, NE 68803
(308) 382-1781
- 41 years experience
- 1 office location(s)
- 3
Lori A. Harkins
Harkins Eye Clinic
830 N Alpha St
Grand Island, NE 68803
(308) 384-9148
- 32 years experience
- 1 office location(s)
- 4
Richard E. Diedrichsen
Eye Physicians PC
220 Broadway St
Fullerton, NE 68638
(308) 536-2268
- 1 office location(s)
- 5
Donald P. Sauberan
Eye Physicians PC
220 Broadway St
Fullerton, NE 68638
(308) 536-2268
- 2 office location(s)
- 6
Michelle L. Gleason
Gleason-Janky Eye Physicians
611 N Diers Ave Ste 2
Grand Island, NE 68803
(308) 381-4733
- 1 office location(s)
- 7
Julie P. Janky
Gleason-Janky Eye Physicians
611 N Diers Ave Ste 2
Grand Island, NE 68803
(308) 381-4733
- 1 office location(s)
- 8
Mark D. Emig
Midwest Eye Care
13500 California St
Omaha, NE 68154
(402) 552-2020
- 4 office location(s)
- 9
John C. Welch
Retina Center Of Nebraska
2115 N Kansas Ave Ste 104
Hastings, NE 68901
(402) 461-4611
- 1 office location(s)
- 10
Paul J. Dietze
Hastings Laser & Eye Surgery Center
715 N Kansas Ave Ste 303
Hastings, NE 68901
(402) 462-5888
- 2 office location(s)
- 11
Gerald F. Geiger
Gerald F Geiger Md
715 N Kansas Ave Ste 300
Hastings, NE 68901
(402) 462-9009
- 1 office location(s)
Don't see your city? Use the WebMD Physician Directory to expand your search.
Eyeglasses, Sunglasses, and Magnifying Glasses
Nonprescription eyeglasses
Nonprescription eyeglasses are primarily for people who can no longer read fine print. This condition, called presbyopia or "old vision," usually begins at about the age of 35 and progre...!--*ICCChunk-2250*-->
Read the Eyeglasses, Sunglasses, and Magnifying Glasses article »
Other Related Eyeglasses, Sunglasses, and Magnifiers Articles
Emergency Contact for Grand Island
- In case of Emergency, call 911
Nearby Grand Island Hospitals results
- 1Saint Francis Medical Center
2620 W Faidley Ave
Grand Island, Nebraska 68803
(308) 384-460
- 2VA Medical Center
2201 N Broadwell Ave
Grand Island, Nebraska 68803
(308) 382-366
- 3Memorial Hospital
1423 7th St
Grand Island, Nebraska 68818
(402) 694-317
- 4Howard County Medical Center
1113 Sherman St
Grand Island, Nebraska 68873
(308) 754-442
- 5Litzenberg Memorial County Hosp
1715 26th St
Grand Island, Nebraska 68826
(308) 946-301
- 6Mary Lanning Memorial Hospital
715 N Saint Joseph Ave
Grand Island, Nebraska 68901
(402) 463-452
- 7Hasting Regional Center
4200 W 2nd St
Grand Island, Nebraska 68901
(402) 462-197
- 8Henderson Healthcare Services
1621 Front St
Grand Island, Nebraska 68371
(402) 723-451
* Provider Directory Terms of Use:
The WebMD 'Provider Directory' is provided by WebMD for use by the general public as a quick reference of information about Providers. The Provider Directory is not intended as a tool for verifying the credentials, qualifications, or abilities of any Provider contained therein. Inclusion in the Provider Directory does not imply recommendation or endorsement nor does omission in the Provider Directory imply WebMD disapproval.
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text:
Source: AutoWeek
quote: CAFE standards were applicable to vehicles under 8,500 lbs AND there "light trucks" were graded differently than "cars". So no, this is not the reason. It's what you believe to be the reason.
quote: Why don't you just look at the historical prices of gas at the pump from 2000-2008 and 2008-2012?
quote: If we are drilling oil locally we can tax oil exports and credit domestically sold oil to make it more cost effective to sell oil that is drilled in the US within the US.
quote: The technology developed and implemented in pinnacle vehicles like the Corvette ZR1 and SRT Viper can be applied to all vehicles throughout the model lineup in some way. So while the investment in developing these cars may not be recouped from sales of said vehicles - the knowledge gained allows them to produce better cars overall.
quote: More simple rithmetic I presume? You're incorrect btw.
quote: We can safely conclude that you have never driven anything other than an econo car or maybe a "sporty import", but noting truly fast like an Evo or WRX STI.
quote: The footprint applies to trucks too because there is quite a difference between the Colorado and the 3500. Now Chevy doesn't have to sell three Colorado to every one 3500 to meet the standard just like they don't need to sell 4 Aveo for every Camaro. That is how the footprint method applies. Read up on it.
quote: Why don't you? Then you can see how it was $4 per gallon right before it plummeted to $2, along with the economy. Wow! Now I understand what you mean, the best way to get gas down to $2 is to crash our economy and kill demand, right?
quote: So we can violate the world trade agreement we signed onto and pay fines to oil producing countries? You can't go protective like you're talking about while remaining part of the WTO.
quote: You're excellent idea is not possible with our economy. As I mentioned earlier, it's simple economics with a demand and supply curve dictated by the rise in demand in China and the limiting of supply by OPEC.
quote: The engines are not investments that can be applied to all vehicles because you would be stupid to put a 426hp engine in a Malibu.
quote: You are comparing a V8 super car to an anemic I4. My original example was a 150hp vs a 300hp. So a better comparison would be your V6 Camaro and the I4 Accent. You add 50% more weight for 60% increase in hp and torque. If there were better examples.I would use them but they are a rough selection. Ofcourse a 425hp car is going to beat a 100hp car unless it is 4 times the weight. I never said an econobox with near equal weight and a quarter of the power would be the same. Your example is more like comparing a Veyron to a Prius.
quote: I've driven a Mustang GT, a Corvette coupe, a WRX STI, and Dodge Charger. I know what cars drive like, and I could easily tell the difference between the Charger and the Corvette based primarily because of the weight difference. Now if you drive those vehicles outside of their proper power bands like 2500 rpms vs 4500 rpms, they feel sluggish like driving a normal mid size car within its power band. That is the point I am trying to make. Any car outside its power band will always feel sluggish because you are slinging a car with little torque relative to its weight.

subset: commoncrawl


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Now showing items 1-10 of 364
UV-stråling i Norge Satellittestimater, modellestimater og bakkemålinger
(The University of Bergen, 2005)
100-year waves in the Mozambique Channel
(The University of Bergen, 2010-06-01)
Six-hourly significant wave height (SWH) data covering the period September 1957 to August 2002 is obtained from the ERA-40 reanalysis. This data, generated by ECMWF's own configured global WAM model, stems from the first ...
Sea level variation in the Red Sea based on SODA data
(The University of Bergen, 2012-10-01)
In this study we investigate the sea level variation in the Red Sea focusing on wind stress setup. 50-years dataset from 1957 to 2007 of sea level and wind stress from Simple Ocean Data Assimilation (SODA) data version ...
Seasonal and Interannual Variations of Surface Nutrients and Hydrography in the Norwegian Sea
(The University of Bergen, 2012-10-01)
Seasonal and interannual variations of surface nutrients and hydrography data obtained in period (1997-2010) by the Institute of Marine Research over three transects: Fugløya-Bjørnøya, Gimsøy and Svinøy located in the ...
The Dynamics of the East Madagascar Current System and Its Influence on the Biological Production Associated to the Shelf - An Observational Study
(The University of Bergen, 2011-06-01)
The dynamics of the East Madagascar Current (EMC) system, and its influence on the biological production associated to the shelf, is studied by using cruise-data collected onboard the Norwegian R/V. Dr. Fridtjof Nansen ...
An investigation of the Faroe Shelf Front
(The University of Bergen, 2003)
The water mass on the Faroe Shelf is distinct from the off-shelf water surrounding the shelf. This difference of water masses is reflected in the temperature and salinity distributions. The on-shelf water is colder and ...
Studies of Eddies in the Marginal Ice Zone Along the East Greenland Current Using Spaceborne Synthetic Aperture Radar (SAR)
(The University of Bergen, 2011)
The characteristic ice edge eddies in the Marginal Ice Zone (MIZ) are studied based on satellite information from the Advanced Synthetic Aperture Radar (ASAR) instrument. The eddies frequency of existence during the day, ...
A study on the processes that cause extreme precipitation on the west coast of Norway
(The University of Bergen, 2011-11-20)
The purpose of this thesis was to get a deeper understanding of extreme daily precipitation events on the west coast of Norway. To examine the extreme precipitation on the west coast of Norway, daily precipitation events ...
Measurement and evaluation of OH-airglow spectra over Svalbard
(The University of Bergen, 2001)
Ensemble-based data assimilation and forecasting with an eddy-resolving model of the Gulf of Mexico
(The University of Bergen, 2008-09-12)


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Box Section Ref ID
December 1914
(Feb. 2, 1914, xvii, No. 3)
Abstracted by R. C. Jamieson, M.D.
treatment of chronic ulcers of the let with frog flesh poultice. Lim Boon Keng, p. 34.
This method has been used by the author with success in treating this condition in coolies and even in the ulcerations of leprosy. The ulcers are cleansed with sterile water, the flesh removed antiseptically from the legs and backs of the frogs, pounded in a clean mortar and applied on muslin. He does not explain its action, but states that it stops bacterial activity and hastens repair.
J Cutan Dis. 1914;32(12):879.
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When it comes to tyres, Continental are known worldwide for being the fastest, sturdiest and most efficient out there.
The Grand Prix 4000S is no exception to that reputation. It can deliver traction and stability using its uniquely developed BlackChili compound coating, whilst delivering race-standard speed every time. The 4000S boasts a new Vectran® anti-puncture layer beneath the surface, making it a reliable endurance tyre. For a tyre that will out-live the competition and see you to the finish line, you have only one choice: the Grand Prix 4000S. - T.H
Click Size Guide To Enlarge
|Tyre Fitment:
Please remember to add postal time (2-3 working days) to obtain a complete estimate of delivery to your door.
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Please allow 10 working days from dispatch of your order before notifying us of any late deliveries.
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Please see our returns policy.
Where reviews refer to foods or cosmetic products, results may vary from person to person. Customer reviews are independent and do not represent the views of The Hut Group.
(51 Reviews)
I bought the 4000 Continental Tire and j, I had a problem with one, it was changed in a very resonable time. thank you syle
Michelin was the brand I used before. Since I switched to Continental Grand Prix 4000S clincher road the last few years I really love this brand. Conti seemed to withstand well all kind of road (ie. Texas Gate in North America, sharp shoulder...) especially wet conditions. I usually clean tyres with wet towels and check after ride.
Soft roll and great handling. Seems very resistant too. I'm very satisfied and I highly recommend this product.
The Continental GP 4000S are by far the most durable tires I've ever run. Having previous ran Michelin Pro 4 Service Course, Vittoria Rubino Pro Slick, Vittoria Zaffiro Pro, and Vredstein Fortezza TriComp these tires have outlasted them all. I'm 4,500km in on my first set and just finally have rotated them due to squaring in the rear. I would have rotated them earlier but I have yet to flat on them. I would put the Michelin Pro 4's ahead of them on cornering and grip on climbing but I had severe issues with cuts and flats with those tires. I typically like to try a different set of tires every time I need to replace them but it's going to be hard to buy anything different with the track record so far. The service from PBK is top-notch and they almost always throw something free in your order. Living in Canada it takes about 9-10 business days to receive. Small orders you typically do not get hit with duty. Probably won't find these tires anywhere cheaper.
Love the Continental GP 4000s, second set I have on my wheels, the first ran for over 5000 km. and the wear dimples were still visible. Very dependable tires. Great service from. Probikekit, their prices cannot be beaten. I live in Canada, and it's a hit and miss with the duty charges, this time it was a hit, and even with the additional charges applied, they came well below the cost if I was to purchase locally.
Great tires for a very good price. Have been using these tires exclusively for the last 10 years.
I've been using these tires for several years now on all my bikes and my son is using them in all his races. They last longer than the competition without lost of performance. Great product!
For the money, one of the best tires I have used. It's given me the confidence to ride long distances without having to worry about getting back home. Long lasting and smooth rolling
Excellent tires. Ran these tires since they first came out and never had any issues. Seem to wear very well.
Amazing price..Amazing tire..Free shipping ..German made..enough said!
Great product 9 riders in our Peloton use these Trye's
as a senior rider I liked some of the lighter race tires but after replacing them a couple of times in a season it gets quite expensive . With my continentals I find the low road friction and durability make this tire a keeper, I can almost keep up with the group now.
Very good product, best price i have ever seen Thanks
I use these for training and club races and find them generally bomb proof. I get about 4 to 5000 km out of a pair easily. They handle great, better than some other softer compound clinchers I have used, which surprised me. Any flats I have experienced are nearly always due to debris ( metal, glass, big road chips). The beat lasting tyre for the money.
I used GP 4000s on my road bike last year for over 5000 km with only one flat. I will be installing them on my son's bike this year as well.
Great service, great price, and great product
Great product and top quality! Always good prices with PBK
these are fine tires. They seldom flat , perform well and long lasting. Where you ride and how to care for them has a lot to do with actual experience. While these are more middle of the road tires I use nothing else. My preference on poorer road surfaces is the 25mm tire. Currently, I am trying 23mm on the front and 25mm on the rear of a set of 23mm Hed wheels.
I have used lots of tires and have settled with the 4000S as I love the way it handles the road. Feels very supple under you and holds the road well also smooth's out rough pavement.
What can I say? I've tried several brands and think that the Continental 4000S is the best that can be bought for all-round biking.
the best tyre I every road. I have two bikes one in Toronto wher e the temp .changes one day to another..and they are the best in all temp. the other bike is in florida and the temp is very hot and they work good to. so the tyers are the best.
Didn't use the tire yet but product came quickly. Purchased many products already the past couple months. Always had great experience. Highly recommend it.
One off the few tire you can ride for 5000 km in any weather.
I switched to the gp4000 last year and immediately liked the feel, smooth, low rolling resistance and good handling. I rode 6000+Kms on them often on rough surfaces. They have held up well, which is why I am a repeat buyer.
Great feeling on the road, I highly recommend them.
Simply the best.
Fast delivery, excellent price!
One word AWESOME. Thanks
As described. Perfect.
Perfect balance of performance and durability.
I bought this tire to replace a Michelin pro race 4 that died after 100 K. I had some kind of a bad luck after a distance of 50 feet on a gravel road. The side of the tire was cut just too much to repair. I decided to go with the GP 4000S and I am fully satisfied. The tire looks stronger and after 200 K looks still as a brand new one. Performance is as good than the Michelin however with more resistance.
Installed these tyres about three weeks ago and have biked about 500 kms. They appear to live up to their reputation and are a huge improvement over the original tyres that came with the bike. I love the fact that they are puncture resistant so I don't have to worry as much about getting a flat when riding with my group. Nobody likes to be held up waiting for a member of the group to change a flat! I'm looking forward to many hours of carefree riding.
Noticed the increased speed immediately from the decreased rolling resistance.
Simply dependable.
Great handling tire also they are very smooth and they seem to role forever on the road. Would recommend them as a good overall tire.
The Conti's you sold me are the best tires on the road in my opinion. They give a cushiony ride, and have great grip around corners and on rough road. They have a great fit and hold pressure longer than other tires. They wear well and last longer than other tires on the market. I've been buying Conti's since I started cycling over 20 years ago.
My first reaction was to give you 5 out of 5 -- I changed it to 4 out of 5 because of the shipping time. Otherwise, you sell great products at a competitive price and have free shipping. You have excellent communication throughout the process, and the product comes in good condition. I appreciate your products and pricing and free shipping. I will tell our friends about your website. Thank you!
I highly recommend these Continental Grand Prix 4000S Clincher Road Tyres or any continental GP 4000 tyres (I used to be on Vittoria).
The Continental Grand Prix 4000S Clincher Road Tires are great, I saw the difference right away. They offer a super smooth feeling. I've had no flats yet. A lot of reviews say the sidewall has problems holding up, but I've done 100 miles on them and had no problems yet. For this price they were a great buy.
The Continental Grand Prix 4000S Clincher Road Tires - 23mm are the best tires I have bought. They are long lasting, hold the road and are always available at a great price. I am in Quebec Canada where the roads are not the greatest. These tires handle a lot of punishment and last.
Bought these to replace a set of Specialized Pros as I couldn't get a decent price on new Pros. I did a fair bit of research and these were very well recommended. I have ridden 100+ miles on them so far and I was immediately impressed by their low rolling resistance and reasonable grip. I've ridden gator skins before and liked the continental line. These are far quicker feeling than the gator skins and feel more grippy to me. It remains to be seen how they stand up compared to the gator skins.
After trying a number of different makes and models several years ago I tried Conti GP4000Ss and have not used anything else since. 1) Great grip, 2) smooth rolling (almost feel soft under the bike - great for chip-n-seal roads), 3) easy to install on the HED Belgium rims I use currently and the Velocity Aeroheads and A23s that I used before, and 3) they LAST: the only time I've had to retire them has been after running over glass once and a nail another time. Even then I've patched the inside with duct tape and used them on the trainer. I have also tried the 25mm version of these and there is a circumference gain over the 23mm, so the 25s don't fit the seat stay clearance at the brake on my race bike but they're great on my cross bike (used for commuting and foul-weather riding). Considering the price that these tend to go for at LBSs around here, PBK has been a godsend for getting these great tires and a great price, and allowing me to shod all my wheels with them.
Love these tires. Excellent handling. Been riding 5% faster (up & downhill). Easy to install. Will be use them again. Will recommend to my cycling buddies.
Great tires! Rolling resistance is very low making for a much faster ride especially downhill! It even made my rides much more comfortable on the saddle as the tires absorb the bumps in the road.
Very good tyre, very perfomant and tougth, almost indestructible. Easy to assemble on wheels. Best purchase on PBK
I've been using these tires about a month now and I love them. I usually ride about 200-300km a week and they have been wonderful. Very smooth rolling and the tires feel like they have me firmly planted on the road. So far they have been flat and puncture free with no wear visible. I highly recommend these tires and will re order them once these wear out!
The Continental Grand Prix 4000S Clincher Road Tyre is an excellent tyre that gives good performance, control and perfect feeling of the road.
So far, so good. I got a pair of these Continental Grand Prix 4000S Clincher Road Tyres to go on my new race wheels. Mounted by hand, with no tools required. They look great and my first impressions on the road have been really good. I haven't had them long enough to form an opinion on wear, puncture resistance or performance in the wet.
Best tires I have ever bought for my bike. Nice grip, smooth ride and control. Will buy more from this site when the time comes.
What a tyre these Continental Grand Prix 4000s are. I changed from the Michelin pro's to these and it made notice able difference. I got a few flat tyres before and got some co 2 cartridges. but when i changed to these tyres i haven't had to use them for over a year now. i am sure i will get a flat tyre some day but they are just amazing. i regularly use my bike and ride about 50km or more every week. i love the handling and the speed they offer. i will not try another after riding on these babies.


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Public invited to participate in deliberative theater event at WQED regarding Marcellus Shale
July 18, 2011
George Hazimanolis
Maria Pisano
Public invited to participate in deliberative theater event
at WQED regarding Marcellus Shale
Wednesday, July 27
4:30 – 8:15pm
WQED Studios
4802 Fifth Avenue
Pittsburgh, PA 15213
Media is invited to attend the dress rehearsal on July 27 at 2 pm at WQED. Representatives from the partnering organizations will be available for interviews and the dress rehearsal can be photographed.
PITTSBURGH—Through a unique partnership between WQED Multimedia, Pop City, Pittsburgh's Unseam'd Shakespeare Company, Carnegie Mellon's Program for Deliberative Democracy, and the Coro Center for Civic Leadership, a year-long initiative to focus on Marcellus Shale and its impact on the community will kick-off Wednesday, July 27 at the WQED Studios in Oakland.
The public is invited to attend Managing Marcellus, an evening of deliberative theater and discussion that will engage the community in democratic dialogue from 4:30 – 8:15 p.m. at WQED.
Attendance is by registration at popcitymedia.com and deadline to register is Wednesday, July 20. Seating is limited and participants will be selected at random, not first to sign up.
Participants will participate in a pre-event survey from The Coro Center for Civic Leadership, watch an original performance from the Unseam'd Shakespeare Company dramatizing issues raised by Marcellus Shale, discuss the production with others in the audience including a panel of local experts and government representatives, and take a post-event survey coordinated by the Coro Center and Program for Deliberative Democracy.
The panel of local policy-makers will include:
• Sala Udin, former Pittsburgh City Councilman and current CEO of Coro Center for Civic Leadership will serve as the evening's moderator;
• Bracken Burns, Washington County Commissioner;
• John Hanger, former Secretary of the Pennsylvania Department of Environmental Protection and President and CEO of Citizens for Pennsylvania's Future (PennFuture) who now serves as Special Counsel at Eckert Seamans law firm and runs Hanger Consulting, LLC;
• and Angela M. Zimmerlink, Fayette County Commissioner and Chair of the Fayette County Marcellus Shale Task Force.
Upon completion of the evening the Coro Center and Program for Deliberative Democracy will develop a final report that will be used to determine the content areas WQED will focus on for future elements of the initiative later this year.
"The goal of this project is to engage the audience in thoughtful reflection and dialogue with area leaders currently working to address the goals and concerns of both businesses in the industry and citizens," said Darryl Ford Williams, WQED Vice President of Content.
Managing Marcellus will be recorded and used as part of a future production to air on WQED-TV in the fall of 2011. Throughout the year several other WQED productions regarding the Marcellus Shale drilling are planned along with a website dedicated to related resources regarding the environment, economy and health and social implications of drilling in the region. Included on this page will be three future webinars facilitated by WQED's education department to include conversations with experts in the fields of geology and engineering, mapping and data capture, legal and financial guidance and public safety and archived there for use by citizens, academia and high school educators.
Funding for this year-long initiative was made possible by a grant from the Colcom Foundation.
In the last year, WQED has produced major content on Marcellus Shale. WQED addressed the issue in a series called What's In The Water in June 2010. In November 2010, Marcellus Shale: The Price of Progress aired as part of WQED's Experience documentary series. Also in November, WQED produced and broadcast a town hall meeting on the subject. In March 2011 WQED produced Drilling for Answers: The Marcellus Shale Debate, underwritten by PNC. Host Chris Moore moderated a discussion with a panel of experts representing the gas drilling industry and environmental groups.
To view these and other related programs viewers should go to http://www.wqed.org/tv/watch/ and search "Marcellus Shale."
WQED Pittsburgh, honored with the 2007 and 2006 Mid-Atlantic Emmy® Award for Station Excellence, was founded in 1954 as the nation's first community-supported broadcaster. The people of WQED create, produce and distribute quality programs, products and services to engage, inform, educate and entertain the public within their community and around the world. WQED Pittsburgh is one of the first broadcasters in the country to be fully high-definition (HD) in its studio and field production capabilities. It is the parent company of WQED-TV (PBS); WQED: The Neighborhood Channel; WQED: The Create Channel; Classical WQED-FM 89.3/Pittsburgh; Classical WQEJ-FM 89.7/Johnstown; local and national television and radio productions; WQED Interactive (www.wqed.org); and The WQED Education Department.
Pop City is a free, weekly online magazine about the people and places advancing the Pittsburgh region. With a subscriber base of more than 30,000 Pop City takes on issues that matter to the community. Pop City was launched in March, 2006 with the goal to change the conversation about Pittsburgh and encourage Pittsburghers to get more engaged with their community.
The Program for Deliberative Democracy strives to improve local and regional decision-making through informed citizen deliberations. The Program is housed at Carnegie Mellon University and is a joint venture between CMU's Center for Ethics and Policy and Pittsburgh's Coro Center for Civic Leadership. The Program develops deliberative procedures for democratic dialogue and decision-making.
The Coro Center for Civic Leadership aims to advance ethical and effective leaders who share a commitment to civic engagement. The Coro Center is building a leadership pipeline to foster collaboration within and across Pittsburgh's business, nonprofit and government sectors. More information on Coro Pittsburgh can be found at www.pittsburgh.coro.org.
Unseam'd Shakespeare Company engages a diverse group of Pittsburgh artists from various disciplines to develop and present innovative new visions of classic plays. As an extension of its artistic mission, the Company's productions are often accompanied by activities that engage the community in a conversation about the themes and issues in the plays it presents.
Colcom Foundation aims to foster a sustainable environment to ensure quality of life for all Americans by addressing major causes and consequences of overpopulation and its adverse effects on natural resources.
| Community Engagement | website


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Contact our customer service representatives and get expert advice for your Dremel tool
Dremel is one of the divisions within the Robert Bosch Tool Corporation, which is the North American brand of the largest power tool and accessory manufacturer in the world, Robert Bosch GmbH.
A world leader in power tools, Robert Bosch Tool Corporation brings together some of the most well-known brands in North America, including: Bosch, CST/Berger, David White, Dremel, Gilmour, Nelson, RotoZip, SKIL, Sunterra and Vermont American.
The international nature of our company provides global opportunities for our associates, while our private business ownership structure fosters a long-term perspective on innovation and creativity in the development and engineering of our products.To search Dremel career opportunities or submit your resume online, visit Bosch USA Careers at the link below, select Job Search then scroll down to select Robert Bosch Tool Corporation. Bosch USA Careers


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Celebrity Central / Top 25 Celebs
Mila Kunis
- 1991
Hello, Hollywood!
At 7, Mila Kunis emigrates with her family (parents Mark and Elvira and older brother Michael) from their native Ukraine to Los Angeles. Though it's a rough start for the non-English-speaking Kunis, she eventually becomes fluent – and her parents enroll her in a children's acting program. "We thought it would be a great way for Mila to meet some new friends," her mom later tells PEOPLE.
- 1992
What a Doll!
While studying at the Beverly Hills Studio, Kunis, 9, meets Susan Curtis, the woman who manages her career to this day. "My parents told her, 'Listen, we can't afford headshots; we can't afford anything. We can't take her to auditions because we work full-time,'" she says of her early career. "[Curtis] said, 'Don't worry. I'll fix everything,' and she did." Soon the photogenic newcomer appears in ads and TV commercials for Barbie, Lisa Frank products and Payless shoes.
- 1998
Time Travel
At 14, Kunis stars as snobby Jackie Burkhart in That '70s Show, with Ashton Kutcher, Topher Grace, Danny Masterson and Laura Prepon. The series, which becomes the second longest-running sitcom on FOX, makes Kunis, for better or worse, a household name. "Kids come up with magazines and ask me to sign them," she says of her classmates at Fairfax High. "When I'm trying to fit in, that doesn't help."
- 1999
'Meg'-a Star
Relying on just a squeaky voice and comedic timing, Kunis steps in for Lacey Chabert to voice Meg Griffin in the satirical cartoon Family Guy. Creator Seth Macfarlane slightly rewrites the character for Kunis, whom he says lends a natural, authentic vibe to Meg.
- 2002
Mila's Rock Steady
Kunis quietly begins dating Home Alone star Macaulay Culkin. "We grew up together. You find a steady rock in your life and that's all you need," Kunis says of her actor boyfriend in a rare 2009 interview. "We have our ups and downs, but work through them." In early 2011, Kunis's rep confirms that the couple called it quits after eight years together.
- 2008
Aloha, Big Screen!
Kunis steals the show opposite funnymen Jason Segel and Russell Brand in the comedy Forgetting Sarah Marshall, where she glows as sexy hotel concierge Rachel, prompting EW to ask, "When did Mila Kunis grow up so good?" The big screen hit (it opens No. 2 at the box office) allows the actress to challenge herself with roles in Extract and the Denzel Washington vehicle Book of Eli.
- 2010
'Swan' Song
Kunis presents the perfect foil for uptight ballerina Natalie Portman in Darren Aronofsky's macabre thriller Black Swan, earning Kunis her first supporting actress Golden Globe and Screen Actors Guild Award nominations. "Aesthetically, I had to look like a ballerina and hold myself like a ballerina. By the end, I was 95 lbs.," the actress says of her transformation, which required her to train seven days a week for seven months.
- 2011
More Than 'Friends'?
After shooting the comedy, Friends with Benefits, Kunis is "pretty inseparable" with onscreen love Justin Timberlake at a Screen Actors Guild Awards afterparty in Los Angeles, prompting sources to insist that the two are just friends – without benefits.
- 2012
That '70s Connection
Kunis, 28, is spotted spending time with former That '70s Show costar Ashton Kutcher, dining in Los Angeles and escaping on a three-day getaway to the coastal town of Carpinteria, Calif. While a rep for the actress dismisses rumors of a romance, the pair go public with their relationship, attending a wrap party for Kutcher's upcoming biopic Jobs. "They stayed within five feet of each other practically the entire evening," an onlooker tells PEOPLE. "It looked like young love."
Bear Essentials
Reuniting with Family Guy creator Seth MacFarlane, Kunis stars alongside Mark Wahlberg in Ted, a crude comedy about a man's relationship with his magical and foul-mouthed teddy bear. The film takes the top spot at the box office, earning $54.1 million during its opening weekend.
Photo Credits
BIOGRAPHY (top to bottom): Courtesy FOX; John M. Heller/Getty; Chris Polk/FilmMagic; Courtesy Universal Studios; Niko Tavernise; Jose Perez/Splash News Online; X17online; Universal Studios
- Name
- Mila Kunis
- Real Name
- Milena Kunis
- Date of Birth
- August 14, 1983
- Birth Place
- Chernivsti, Ukraine
- Relationships
Ashton Kutcher, fiancé (2012 to present)
Macaulay Culkin, ex-boyfriend (2002 to 2010)
- Children
- Wyatt Isabelle Kutcher, daughter (9/30/14)


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Help Us Inform Your Community
The Advocacy Team has been hard at work with your help passing oral chemotherapy parity legislation and now we are taking it one step further.
We have developed an educational tool that gives an overview of each state law and the impact the law will have on patients as well as information about who to contact if your insurance company isn't complying.
We want to make sure that your clinicians, nurses, and fellow patients are kept up-to-date on the laws in your state, but we need your help to spread the word! Read More


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Several Dallas hospitals get poor marks in new safety report
A new safety study from the health policy wonks at the Leapfrog Group gives poor marks to many of Dallas' biggest and most important hospitals.
Using a letter-grade scoring system, Leapfrog's report gave a C to Parkland Memorial Hospital, UT Southwestern Medical Center's St. Paul and Zale Lipshy hospitals, and Methodist Health System's Methodist Dallas Medical Center in north Oak Cliff and Methodist Charlton Medical.
Medical City Dallas did receive an "A" while1 Texas Health's Presbyterian Hospital and Baylor University Medical Center near downtown each scored a respectable B.
That showing echoes findings from our analysis last year of patient data to measure the frequency of potentially preventable complications at large Texas hospitals. Many North Texas hospitals scored poorly in our statewide analysis — and Parkland, UTSW's St. Paul and Methodist Charlton were among the worst.


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9 Types of Job that Will Destroy Your Soul
#4. The Lie Bot
Also Known As:
The job where you have to lie right to people's faces, to make them buy things.
Some of you immediately said, "What, like lawyers and politicians?" But those are careers. Big, big difference. Those people choose that, and went to school for it.
No, I'm talking about jobs. The type of position you take just to pay the bills until you can start your rap career.
For Example ...
Almost any sales job, for one. Think about the poor bastard at Best Buy getting paid hourly to pretend Monster brand cables aren't a scam. Like working on cars? You'll be shocked to find how many repair shops pressure you into padding the bill with bullshit repairs and worse. Maybe you took a general customer service job at a phone bank, but then they contracted out to some shady software company that has you cold calling people to get them to buy some malware-loaded bullshit that will "speed up their computer."
And while the "sleazy used car salesman" is a stereotype, it's not like they have a sign up front that says, "Now hiring shitheads." Car sales is one of the precious few jobs on planet Earth where you can come in with no experience but still make decent money. Yet, part of doing the job is learning to perform that staged bullshit where you pretend to consult with a manager to get the customer a lower price.
"He said, 'Go fuck yourself.'"
You'll find that lots of jobs that sound great in the classifieds secretly have "being a shithead" in the job description -- that's how they find people willing to do it. MLM sales, door to door sales, telemarketing jobs, all can have you selling shady, shamefully overpriced or downright fraudulent bullshit.
And what makes those jobs even more awesome is that you in no way restrict the lies to gullible rich people who could maybe afford to lose the money you're stealing. The rich people have lawyers -- you stay away from them. No, the job of the Lie Bot is usually to hustle single moms out of next months' diaper money.
Via Singlemomfinancialhelp.com
Though I could probably get a couple hundred bucks for that kid.
#3. The Rat in a Cage
Via Batguys.com
Also Known As:
The manager or supervisor who has no authority to actually manage the employees under him or her, yet is responsible for their performance.
There is this huge, obvious, yet shockingly common flaw in modern businesses. You'll have the employees who actually make the stuff or perform the service. Then right above them is a supervisor (you), who is ultimately responsible for the performance of those employees (or at least, you'll catch hell when they slack off). But you do not have the power to fire them. Or reprimand them or reward them or punish them or give them the smack with the back of your hand that would send them tumbling to the floor that you dream of giving them each and every night.
"Gabe" from The Office is one.
For Example ...
Maybe you're at a family business, but you, the supervisor, are not a member of the family. But the kid working under you, the one who is scanning his balls into the copier, is a member of the family and is thus untouchable. Or, maybe you don't have firing authority because of some complicated union situation.
Or, maybe the workers are working in your department but not for your department. Say you're a supervisor in Sales, and the guy the IT department sent over to work on your computers is a dipshit. So he's cursing while you're on the phone with clients, he's drawing boners on your family photos, he's dicking off and meanwhile, you can't use your computer to do your job. And you can't say anything because while you're a supervisor, you're not his supervisor.
Sure, you can complain to his supervisor, but you can't make him take action -- that guy is at the exact same level as you. Maybe he's friends with the dipshit, or maybe all the IT guys look out for their own, and all the next day you'll hear them chuckling about it when you pass their office.
In other cases, it's just a matter of the person who could actually fire or suspend incompetent workers not working in the same building, or even in the same city, as the dipshits. You send your complaints up the ladder and they disappear into the clouds somewhere. Then, one day you realize the shitty worker you're complaining about has worked there longer than you and that 20 years from now, they'll still be there.
In the end, all you can do is verbally compliment the performers and beg the incompetents to do better. That, and continue to twist your stomach in knots every time they screw up, watching them ruin your career, utterly powerless to stop it.
Your life ... totally under his control.
But in case it sounds like I was being too hard on the IT guy back there, let's hear it for ...
#2. The Assistant Cromulationist
Also Known As:
The highly technical job that is impossible to explain to those both inside and out of the workplace.
If you used to watch Friends, you remember the running joke about how nobody knew what exactly Chandler did for a living. He was always exasperated by this ("I told you, it's statistical analysis and data reconfiguration!").
Like the Laughingstock, the person with this type of job physically cringes at the thought of having to answer the "So what do you do?" question, and eventually invents a fake job title or a ridiculously dumbed down version ("I work on computers") for conversation purposes. And if awkward conversation was the only problem, it wouldn't be that big of a deal. The real problem is when none of their co-workers understand their job either.
"I ... well ... I put stuff in water."
For Example ...
We have a disproportionately computer literate audience, and I know a lot of you aspire to work in the field. Well, some of you are going to wind up as the one-man computer tech support team in an office full of old timers who still regard computers as a suspicious, yet necessary form of black magic. Maybe you'll be the guy who maintains the online orders, in a department where everybody else hits the road and sells the old-fashioned way.
This is any job where the other employees' task is labor intensive or requires "real world" work, and you're just sitting there "playing on your computer." That's the key; because they don't understand what you do, and because you aren't capable of explaining it so that they'd understand, they tend to assume you're just jerking off all day.
Even if they're right.
So, they start treating you like dead weight. When profits are tight and it comes time to cut staff, everyone will point the finger at you. If lovable old Frank in Sales gets the ax instead, everyone will resent you even more ("They fire a hard-working veteran like ol' Frank, but they keep Dave just because he can use the fancy computer machine?!? He don't even wear a tie to work!")
And that's assuming that the people doing the firing also aren't confused about your value to the company. If your job is, for instance, to prevent a problem that the average person isn't even aware of, then good luck explaining that to the guy who has to make layoff decisions based on how much profit you're bringing in. Think of the frustrated employees in Office Space trying to justify what they do to "the Two Bobs" (the two downsizing consultants, who both happened to be named Bob).
Via Cio.com
And most companies employ a couple of them.
Then again, being a Bob isn't exactly a sweet gig ...
#1. The Bob
Also Known As:
The one whose job it is to make everyone else's jobs harder, or to recommend they be fired.
On some level, we all realize there is often a big fucking gulf between what workers enjoy, and what actually makes a company profitable. Some of what the company needs the workers to do is going to piss the workers off, and somebody has to make them do it. And that's fine, as long as that guy is the boss. But that's often not how it works. Often the person cracking the whip is a Bob.
Via Oregonstate.edu
"How do you spell 'Fuck off, Chad'?"
This is an employee who is either on the same level of the rest of the staff, or they're temporarily elevated to some kind of task force (to raise quality or whatever), or they're outside consultants brought in to shape up the operation, like in the Office Space example.
But one way or the other, if you're a Bob, you're a traitor. The employees don't work for you, your name isn't on the door, you don't write the checks, you don't have the ability to pay them a sweet bonus. Yet, you have the power to make their lives miserable.
"I'll be working with you for the next couple of weeks. Where can I put my giant face?"
For Example ...
I'm going to use a term here. Some of you won't recognize it. The rest of you will reflexively feel your genitals crawl up into your body:
ISO 9000 is a certification that businesses can get that declares they have their shit together. Which sounds great, but from the employee point of view, ISO 9000 means a task that used to take two mouse clicks now takes two mouse clicks and three pages of exhaustive forms explaining what they just did. It's endless, hellish record-keeping. Getting certified means ISO 9000 consultants come into the office and hover over every employee, constantly reminding them to log their time and fill out their forms.
Exactly 100 percent of the things the consultant is telling the employees to do involve making their job much, much harder. When they leave, ISO compliance will be handed off to someone within the company. And everyone will want to murder them. They're now a Bob.
"No, he's just hovering around, staring at me like a fucking idiot."
But the key is you could get promoted to a Bob job tomorrow. Maybe you'll get asked to work in Quality Assurance, recording and reporting your fellow workers' errors. Maybe you'll be put on a team to create a report about the department's "efficiency."
Or, maybe you'll have to go review the work some other company is doing for yours on a contract basis, and you'll be the Bob over there. Like the toy company representatives who hang around movie sets and make sure that from the script stage on, the characters will make good action figures. You know that artists and storytellers love to hear that shit. "Sure, the character is endearing and resonates with the audience and has a compelling story arc, but you need to give it hands so we can sell accessories for it to hold."
What are they going to do, complain to their boss? Their boss is the one who paid you to come tell them that. And you're just doing your job.
That's what I guess you have to keep in mind, because a whole lot of the people on this list could be spun off into an article about "People at Work You Want to Murder but Can't Because It's Illegal." Just remember, they're not villains. They're just people trapped in shitty jobs.
Thanks to everyone in this thread for sharing their work horror stories, and toChristina H., Pauli Poisuo, Daedalus, Luna Fortuna, SirMrSir, Something Clever and, uh, "Flamer Shaftglutton" for their examples.


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Top Definition
Of Yiddish and New Zealand origin, meaning a large amount of hype or fuss for very little, or diminishing, returns.
1. Despite all the schvizzel about the party beforehand, it turned out to be a dud.
2. There was much schvizzel about nothing.
2. There was much schvizzel about nothing.
από gurdyp 11 Ιανουάριος 2010
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No signature canes - suggested makers include Clichy or St Louis.
***I was watching this battered little Silesian / Thuringian weight on eBay, and was astounded at the price it sold for. Did the buyer believe it was Clichy?
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8 Bad Drivers Nobody Complains About (Part 2)
#4. People Who Leave Farcically Generous Gaps In Front
Now I realize it is a safety thing to leave big gaps in front of your car when you are moving and we have all read the scary DMV brochures about how it takes almost 300 feet to stop at max speed or whatever, but when you are stopped, do you know how long it takes to stop and avoid running into the car in front of you? Zero seconds. Because you are stopped. You can go ahead and get close.
Despite what some people think, it is safe to get within 30 feet of a stopped car, maybe even 10 if you are feeling like a daredevil and put some banana slices in your oatmeal this morning for a change of pace. Sure, someone could go into reverse randomly and back into you I guess, but those are crazy person odds, same as your chances of getting stabbed by a co-worker who suddenly started hearing Joan Rivers' voice telling him to purify the earth.
And who wouldn't do it? Just to make the voice stop.
This kind of behavior is annoying everywhere, but especially if the gap-leaving car is blocking you from getting somewhere you could easily go if he moved up to a reasonable distance behind the next car. Like into a left-turn lane.
And there are two types here -- there's the person who genuinely thinks they should be 40 feet behind the car in front of them because that's safest, and then there's the person who stopped at a reasonable distance and then stopped paying attention while the cars in front of them moved up quite a bit. The second person's not a problem if they look up and move, but what's baffling is people who don't seem to be doing anything but just sitting there.
Maybe they're gas misers who don't want a single stop-and-start more than necessary, or maybe they're daydreaming about sex. They always say men think about sex every 7 seconds, so I guess it's plausible some guy is sitting there imagining boning someone while traffic moves up around him.
#3. People Who Take Stop Signs Too Literally
On the one hand, this person is at least halfway there and understands that you ought to stop at a stop sign, putting them far ahead of many cars and bicyclists who just see the signs as colorful street decorations.
On the other hand, stopping at a stop sign is just step 1. After that, you have to assess who has the right of way, and go only when it's your turn. Many people seem to miss that second step, and after coming to a stop, just go, regardless of whether there are any other cars present and what they might be doing.
At a four-way stop this usually just leads to anger and confusion (which I believe leads to the Dark Side) since the other cars are starting from a stop and are usually going slow enough to avoid accidents. At a two-way stop, the literalist driver can end up plunging ahead into rapidly-flowing traffic and will be lucky if they don't cause an accident.
A friend of mine actually ran into one of these people who pulled out unexpectedly in front of her, the other driver being completely bewildered afterward as to what she did wrong. "But I stopped!" she said, having apparently had complete faith that anything that happened after the stop would be somehow handled by the omnipresent Traffic God.
I don't know if there is a way of getting through to someone like that, but if you know any of those people, explain that stopping at a stop sign is not a religious ritual designed to appease the gods, so that they might have their blessing to enter the intersection, but is a practical step to let you see if any other cars are coming and figure out the proper time to go in.
Or just tell them the gods desire them to look and let the non-stopping traffic go first, whatever.
#2. Inchers
I'm betting these are the same people who fidget incessantly in person. They can't stay still when sitting in a chair, so why would they when sitting in a car? When stopped at a red light or stop sign or other delay, they scoot forward inch-by-inch impatiently even though it's completely outside their control when they'll be allowed to move forward next (when the light turns, when traffic moves forward).
Maybe they're control freaks who hate not being able to affect their fate. Maybe they're just fidgeters who do it out of habit. Either way they suck and they should stop doing it. Not only does it not help them go any faster, in some cases it might make the wait take longer, as in the diagram above.
That dipshit is waiting for a car to pass so he can turn right. He can't keep his damn foot still, so he scoots forward even as the car is coming, even though he knows damn well he can't do anything until the car is past. This startles the oncoming car because he doesn't know if this is one of those stop sign idiots that just drives right out into traffic, so he hits the brakes. End result? Our impatient dipshit has to wait even longer for the car to pass.
If he'd just sat quietly, the car would have continued past at full speed. And that's just one car. Startling and slowing down a car in busy traffic could mean he could actually lose the rare gap he was planning on turning into.
Most of these drivers think of themselves as fast, smart drivers ready to jump into any opportunity, and will probably think it's other drivers' fault for startling and thinking that they would be dumb enough to pull in front of traffic. That kind of tells you how wrapped up in themselves they are because drivers can't read minds and nobody puts their fucking ID on their hood. Your smart, narcissistic opportunist and your slow, unaware bad driver both look exactly the same on the road -- like a car surging forward toward fast cross-traffic.
People who think their smartness and great driving skills should be obvious to someone in that circumstance must have their heads pretty far up their own asses. Chances are a lot of them are also ...
#1. People Who Think It's Their Job To Teach People "Lessons"
I'll put this here as a caveat for anyone who read any of the other bad driver types I mentioned and are motivated to "teach them a lesson" on the road -- and that's not an uncommon attitude. These lesson masters wouldn't dare think of themselves as one of those "road rage" people, and separate themselves from road ragers in their mind because they seem themselves as an above-it-all authority who is using some clever method to "help" other people learn and "make them think," as opposed to being out for petty revenge, like a road rager.
Here's a mild example of that thinking in action:
You know why that person did not seem to notice? Because they were trying to call 911 on their cell phone. "I'M OUT IN THE MIDDLE OF NOWHERE ALONE WITH THIS DRIVER AND HE'S TRYING TO DO SOMETHING TO ME!" By the time they start moving in front of you and behind you and in front of you, you stop guessing harmless little causes like the blinker and start looking to see if they have a hook for a hand.
Or if you can see a lot of rope and duct tape in their car.
At least this only involved passing, though. Some people try more elaborate lessons that could very well lead to an accident, taking them beyond the realm of being a stupid snob and into real jackassery. One example: Some drivers wait until the last minute to merge, bypassing a long and slow line. This annoys me as much as the next person, but some people feel the urge to show that driver how much he has inconvenienced people by tailgating him after, or even during, the merge, and continuing to ride his tail a good long time after, to make sure he notices.
Others like to "teach" a tailgater how dangerous tailgating can be, by suddenly hitting the brakes. If they learn the lesson too well, they could both end up discussing it further on the side of the road while waiting for the tow truck.
Again, a lot of these people like to separate what they're doing from road rage by pretending they're teaching people something, but first of all, they're clearly pissed and lying to themselves, and secondly, both ways indicate a person who is way too high on himself, whether you think you deserve personal revenge, or whether you think you're such an authority that you should judge and teach other people like you are Freeway Jesus.
Either way, you are angling to get shot. If you meet any of these annoying drivers on the road, don't be a goddamned road vigilante, just come here and complain about them on the Internet.
For more from Christina, check out 6 Ways Cities Are Getting Into the Attention-Whore Game and 6 Groups Who Don't Work as Movie Bad Guys Anymore.


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NSCLC is "Major Battlefield" as Merck,...
LC-MS Gains Favor Due To the...
Wall Street Decline Takes Its Toll...
Expanding Use of NGS Has Largely...
Five presentations on protein purification at last month's "Bioprocess International Europe Conference" in Prague provided clear and detailed insights on the latest thinking on how to best handle therapeutic biomolecules.
Günther Jagschies, Ph.D., strategic customer relations leader at GE Healthcare, reviewed the pluses and minuses of affinity chromatography for both monoclonal antibodies and "novel applications." These include purification of kappa and lambda Fab fragments, coagulation factors VII and VIII, the nonpathogenic adeno-associated virus, and alfa-1 antitrypsin. Competition has heated up for protein A resins, with several small players introducing new mAb capture resins with binding capacities approaching 60 g/L with low residence times and high caustic stability.
Most experts agree, and actual practice validates, that affinity chromatography is the capture step of choice for mAbs due to its high selectivity, volume reduction, suitability for platform separations, and reduction of subsequent purification steps.
"But cost becomes an issue when the capacity and lifetime features of affinity resins are not fully utilized, such as during clinical manufacturing or for products requiring a limited number of batches per year," said Dr. Jagschies.
Correctly sizing a capture column, particularly for legacy mAbs, is the most straightforward way to minimize cost issues, he added.
Ligand leakage and resin lifetime were considered issues in the past, but are rarely problems with today's well-designed and -supported resins. Subsequent purification steps almost always clear ligands, which in any case do not lead to significant capacity losses. Lifetime has reached the low hundreds of cycles for hydroxide-stable ligands and the fifties for resins that are not as stable to CIP.
Although costs dominate discussions of affinity resin negatives, according to Dr. Jagschies, interest in protein A and other affinity-based separations has never been higher. This is somewhat of a contradiction since costs dominate, especially at lower scale. "I do not believe that cost will prevent manufacturers from using affinity capture as it is too powerful a method to miss out on," he explained.
Xuemei He, Ph.D., a process chromatography expert at Bio-Rad Laboratories, discussed a new three-column workflow for purifying a monoclonal IgM antibody. Because the protein was unstable below pH 5 and prone to aggregation, cation exchange and hydrophobic interaction chromatography was problematic.
By taking advantage of the novel mixed mode medium's salt tolerance, Dr. He was able to first remove DNA and host cell proteins by flow through chromatography over AG1-X8 resin followed directly by binding the antibody onto the novel mixed mode medium at high salt and close to neutral pH. The third step, polishing with a ceramic hydroxyapatite (CHT), gave active protein free of aggregates.
Purifying IgMs is challenging due to the molecules' large size and instability. Several capture modes have been tried, including ion exchange, HIC, size exclusion, and affinity chromatography. IgMs often denature or precipitate under these conditions.
Although size-exclusion chromatography is gentle it does not exhibit production-scale volumetric productivity. Affinity chromatography media for IgM purification are currently available, but the molecules frequently do not survive the harsh elution conditions, resulting in low recovery.
IgMs tend to be more stable in high-salt buffers, which makes them unsuitable for conventional ion-exchange purification. Dilution to reduce feedstream conductivity for IgM binding on an ion-exchange column may lead to target molecule precipitation, while the higher process volumes are problems in their own right.
Dr. He's approach works around IgM's large molecular mass and heavy glycosylation, which lead to slow diffusion through conventional media. Her development-stage resin is based on rigid macroporous polymeric base matrices that allow efficient mass transfer. The precise chemistry involved is still under wraps. Dr. He did disclose that it contains "structural elements that interact with target molecules via one or more modes of interaction that include one or more electrostatic or hydrophobic interactions and hydrogen bonding.
"Under optimized conditions, this mixed-mode media offers unique selectivity unmatched by single-mode chromatography."
Bio-Rad is currently working with collaborators to develop an intuitive approach to method development using this medium.
CHT is the ceramic form of the ages-old, naturally occurring mixed-mode chromatography material, hydroxyapatite or (Ca5(PO4)3OH)2. Electrostatic interactions between calcium and phosphate groups and the surface charges on biomolecules are what make CHT the "polishing workhorse" for removing process- and product-related impurities such as host cell proteins/DNAs, viruses, endotoxins, leached ligand from affinity chromatography, and antibody aggregates/fragments.
"Our method has general applicability to a potentially large variety of proteins, particularly where affinity capture is not a viable option," explained Dr. He. But depending on the specific process and proteins the sequence of chromatographic steps and purification conditions may need to be adjusted to achieve the best separation.
"The final process based on this medium is simple, with minimal conditioning steps, yet offers products with high purity and biological activity," said Dr. He.
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Novum Pharma recently raised the price of an acne cream by over 3,900% in less than a year-and-a-half and Mylan increased price of EpiPen from $100 to $608 . Do you think pharmaceutical companies need to be subjected to price controls?
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As Immunotherapy Revolutionizes the Cancer Treatment...
Expanding Use of NGS Has Largely...
LC-MS Gains Favor Due To the...
NSCLC is "Major Battlefield" as Merck,...
Array-based comparative genomic hybridization (aCGH) has become a standard method for detecting copy-number variations (CNVs) and other genetic defects associated with genetic diseases. Once primarily the domain of postnatal testing for developmental disorders, the technique is branching into prenatal (and preconception) testing and cancer.
aCGH involves labeling test and reference genomes with differently colored dyes, mixing them, then hybridizing the samples to a microarray of probes covering a genomic area of interest. The scope may range from a few hundred genes to the entire genome.
aCGH differs from standard CGH in its resolution. Because conventional CGH hybridizes samples directly to chromosomes, resolution is limited to five megabases or so. Arrays employ not whole chromosomes but probes consisting of 50 to 75 bases. The densest commercially available arrays today consist of between two and three million probes. More common are 180,000-spot slides, which provide whole-genome analysis at a resolution of 5–10 kilobases.
"The advantage of microarrays is that they allow viewing of the entire genome as well as regions of special interest," said James Clough, vp for clinical and genomic solutions at Oxford Gene Technology (OGT).
Founded by Edwin Southern, Ph.D., the Oxford professor of Southern blot fame who also invented microarrays, OGT began to provide microarray services about seven years ago. "It soon became clear that array CGH had a role to play in the investigation of constitutional disorders," added Clough.
At the time karyotyping was king, and oligonucleotide arrays were thought to be too noisy. But eventually, researchers found clinical relevance beneath signals originally ascribed to noise. But what eventually won over researchers was the clinical yield: just 5% to 8% for karyotyping, up to 17% on BAC arrays (an early embodiment of oligo arrays), and up to 22% for aCGH.
The most pronounced limitation of aCGH is the inability of the native technique to detect balanced translocations in which precisely the same quantity of genetic material switches from one chromosome to another, and vice versa. Probes can only detect the relative amount of material corresponding to a genetic region, but not its actual location. Balanced translocations are associated with many solid and hematopoietic cancers.
Another limitation is that aCGH cannot distinguish triploidy, tetraploidy, or any other abnormality where every chromosome in the cell is detected in multiple copies, generally termed polyploidy.
"These rearrangements can only be detected using conventional chromosome testing techniques like karyotyping," said Gary Harton, head of molecular research at Reprogenetics. "However, aCGH can be used to detect the inheritance of unbalanced translocations in embryos from couples that carry these chromosome rearrangements."
At the "Association of Genetic Technologies" annual meeting, PerkinElmer described a work-around for balanced translocations, thereby opening up new areas of investigation for aCGH.
PerkinElmer's approach uses gene amplification of known translocations followed by aCGH analysis. It involves polymerase and primers for the affected chromosomes.
For example, the Philadelphia translocation in chronic myeloid leukemia involves chromosomes 9 and 22. As the primer for chromosome 9 and polymerase amplify the gene, they encounter the sequence from chromosome 22 and amplify that as well. Since only the primer for chromosome 9 is present, one would not expect an amplified signal for chromosome 22. Separately, the primer for the breakpoint on chromosome 22 amplifies the genetic material from chromosome 9.
"Now, with that amplified pool in hand, when we look at the array we see chromosome 22 has a signal where you wouldn't expect one," said Christopher Williams, global market segment leader for oncology.
Further analysis would reveal where the DNA broke apart, which holds significance for both prognosis and treatment.
PerkinElmer is offering a service based on this approach and a new product, OncoChip, which targets more than 1,800 loci associated with hematologic cancers. Onco- Chip provides baseline coverage of one oligo probe every 35 kb, and one probe every 500–5,000 bases in targeted regions.
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A masturbation position, primarily used in the shower to keep from slipping. One hand on the wall, the other hand.. well you know.
Dude, I was jacking off in the shower, and I had to do the Heisman Trophy because the tub was so slippery.
লিখেছেন- Bobbybagpipes 10 de জুলাই de 2008
Trending Now
Can you define these popular missing words?
Top Definition
An award given to the best all-around division 1A college football player every season.
The recent Heisman Trophy winners.
2006- Reggie Bush
2005- Matt Leinart
2004- Carson Palmer
2006- Reggie Bush
2005- Matt Leinart
2004- Carson Palmer
লিখেছেন- Snick and Fsquirrel 30 de মে de 2006
A trophy awarded to the best player in college football.
Jay Bergwanger won the first Heisman Trophy.
লিখেছেন- Fifi McFeef 1 de জানুয়ারি de 2008


text:
Football Renovated Locker Room - Photos and Video
Ball State's football program moved into a renovated locker room this summer and this short video offers fans an inside look at the facility.
The renovated locker room includes new lockers, new flooring and a new sectioned off area for halftimes. In addition, new flat screen televisions and plaques of former players numbers who once roamed the field at Scheumann Stadium are featured.
In addition to the renovated locker room, the university broke ground on the new Ron and Joan Venderly Football Team Complex, which is expected to be completed prior to the start of the 2015 season.
The Cardinals report to Fall Camp July 29 and open the season Sat., Aug. 30 in a home game vs. Colgate at Scheumann Stadium.
Under the direction of head coach Pete Lembo, Ball State managed a 10-3 record in 2013 and participated in the GoDaddy Bowl.


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text:
Shutdown scuttles inspections of hospitals, nursing homes
The federal government shutdown hasn't derailed the nation's new health-insurance program. But it has suspended another crucial layer of protection for patients — routine safety inspections of hospitals and nursing homes.
And Texas is among the states hardest hit. Its nearly $1 million monthly appropriation — the second largest next to California's — to pay for such reviews remains frozen until Congress passes a new funding bill.
States conduct investigations of healthcare facilities on behalf of the U.S. Centers for Medicare & Medicaid Services, the nation's primary overseer of federally funded care facilities. In recent days, states have been under a directive from the agency to limit their inspections largely to cases posing "immediate jeopardy" to patients, and those where a hospital's funding is at risk.
"Complaints that are triaged as credible allegations of immediate jeopardy or harm to an individual should continue to be assessed and investigated," according to the CMS memo.
But several forms of inspections designed to flag patient dangers or systemic problems can't proceed. They include "standard surveys," which are the routine inspections of nursing homes and hospitals, and "initial surveys" to gauge whether a care facility being proposed can receive Medicare funding.
States also are prevented from investigating complaints alleging violations of federal regulations that don't rise to the level of immediate danger.
A furloughed federal official said even those complaints can be windows into "bigger issues." Tips about failures of healthcare facilities to properly staff infection control units or investigate medical mistakes can ultimately expose patient harm, the official said.
So far, Texas has had to delay extensive inspections of as many as five hospitals, according to a spokeswoman with the Texas Department of State Health Services, the state agency regulating those facilities.
"We were just beginning to plan our federal workload when the shutdown occurred," said Christine Mann of the state health department.
Texas regulatory officials said some of the oversight gaps created by the shutdown can be covered by other state oversight processes. Many of the state's licensing rules for hospitals, for example, overlap with federal regulations. And Texas inspectors often perform state licensing surveys at the same time they conduct inspections on behalf of CMS.
Follow @milesmoffeit and @DMNInvestigates on Twitter. Like the DMN Investigates page on Facebook.


text:
on February 15, 2013 - 6:04 PM
LOCKPORT – Ashley E. Bivins of Niagara Falls pleaded guilty Friday in connection with the Sept. 15 holdup of the Gulf gas station at Walnut and Washburn streets, Lockport.
Bivins, 22, of 21st Street, admitted to attempted third-degree robbery and was scheduled for sentencing April 17 by State Supreme Court Justice Richard C. Kloch Sr.
She was the fourth person to plead guilty in the case. Last month, Kloch sent James McClendon, 50, of 21st Street, to prison for 1½ to three years.
Lockport City Judge William J. Watson will sentence the other two defendants, both from Niagara Falls. Chelsee Williams, 18, is to be sentenced March 13 for third-degree assault, and Jessica A. Burgess, 28, March 27 on her plea to attempted fourth-degree conspiracy.


text:
It's been one year since Superstorm Sandy pummeled the East Coast. Take a look back at the storm by the numbers.
Superstorm Sandy by the numbersUPDATED 6:47 PM EDT Oct 31, 2013
Slideshow Central
CommentsThe views expressed are not those of this site, this station or its affiliated companies. By posting your comments you agree to accept our terms of use.blog comments powered by Disqus


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Customized items can be personalized with options such as your name, your favorite number, and/or designs. Some options may be limited by league rules.


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This Luxury Lodge Safari Collection offers Kruger Park vacation packages that promise remote peace and tranquility, a taste of history, a memorable luxury tent experience or shameless opulence in an exclusive safari lodge. With an unrivaled abundance of game viewing opportunities, these travel packages promise a Kruger experience you will never forget.
3 day - Luxury Self-drive Safari Perched on a cliff in the middle of nowhere, with views over the river as far as you can see, this ultra-chic retreat is yours for the duration of this 3 day Kruger Park vacation package. Ideal for honeymooners because of its seclusion it is also perfect for birding aficionados due to its location in the northern reaches of the park.
3 day - Luxury Fly-in Safari Lazing in your own jacuzzi on your private viewing deck is a good excuse to do nothing on this 3 day Big Five safari package in Kruger National Park, which is especially recommended for those seeking serenity in Utopia. Take in the stillness of the South African bush from your beautifully appointed suite or enjoy the wilderness on game activities.
3 day - Premier Southern Kruger Safari A Big Five safari experience with 5 star luxury accommodation thrown in for good measure makes this 3 day Kruger Park vacation package a definite 'must do' if you want to really experience the southern section of Kruger Park in style. Participate in an interpretive bush walk with your expert guide who will show you how to 'read the bush'.
3 day - Premier Kruger Safari Reminiscent of Ernest Hemingway's early days in Africa, this luxury tented safari experience is a 3 day Kruger Park vacation package to write home about. Enjoy the enchanting romance, style and great river views from your private deck. Here you will be tempted to take time out after an exciting Big Five safari adventure to just laze and relax.
3 day - Deluxe Fly-in Safari Accommodation is in one of the earliest safari lodges in Kruger Park. On this 3 day package, not only will you encounter the Big Five, but almost feel what life was like in the pioneering days of the gold rush, to be on the old wagon route between the mines and Delagoa Bay on the Indian Ocean. Read the story about Jock of the Bushveld after an exciting safari.
3 day - Premier Tented Safari Spoil yourself on this 3 day luxury tented camp safari experience in a private concession in Kruger National Park. Accommodation is in elegant en-suite safari tents. Your safari will include morning and evening game drives, during which you will enjoy excellent wildlife sightings.


text:
For traditional recipes from the Mysore region and food from all over, with ramblings about life and kids thrown in the mix.
Wednesday, February 22, 2012
Hurali Idli - Horsegram Idlies
2 cups idli rice
3/4 cup horsegram
1/2 tsp methi seeds
a handful of poha
water for soaking and grinding
salt to taste
Soak the rice/methi and horsegram in water separately for 5-6 hours. Soak the poha for about 15-20 mins, before grinding.
Grind the horsegram first to a smooth batter, adding a little water. Then grind the rice, methi and poha and add to the horsegram batter.
Add salt and mix them all together.
Let it ferment overnight (or 8-10 hours).
Grease idli moulds and steam for 10-12 minutes.
Serve with chutney or sambar.
9 comments:
- Anonymous said...
Diid not know idlis could be made with horsegram too... I must try this weekend! DId the batter rise as the usual rice - urad batter? Any precautions to get the fermentation right? thanks. Rajani.
- February 23, 2012 at 9:30:00 AM EST
- Prathibha said...
ur horse gram idlis reminds me that ,I hv a pack of horse gram sitting in freezer which is to b used..nice idlis,I also do this
- February 23, 2012 at 10:18:00 AM EST
- Prathibha said...
ur horse gram idlis reminds me that ,I hv a pack of horse gram sitting in freezer which is to b used..nice idlis,I also do this
- February 23, 2012 at 10:18:00 AM EST
- Manasi said...
These look fantastic and what surprised me was, no urad daal!
I am so bookmarking this and will hop over tot he Indian store to get kulith. Thank u:)
- February 23, 2012 at 10:48:00 AM EST
- Sumi said...
Super healthy Idli..unique recipe.Thanks for sharing
- February 23, 2012 at 6:32:00 PM EST
- Cham said...
We make rasam with horsegram and few other items weekly once, but idlis are super cool one never even thought :) Excellent!
- February 23, 2012 at 8:25:00 PM EST
- Vani said...
Rajani - yes, the battier does rise but not a whole lot. The trick is to grind the horsegram really smooth and the rice mildly coarse. The poha also helps in the softness. Do let me know how they turned out.
Prathibha - oh the stuff one discovers in the back of the freezers! Glad my post reminded you of yours :)
Manasi - yup, no urad dal and still soft :) Tastes different though, with horsegram. But in a nice way.
Sumi- thanks :)
Cham - and I make dosas and adais with them too. Comes out good.
- February 23, 2012 at 10:20:00 PM EST
- Jayashree said...
I like the earthy taste of horsegram. Haven't tried using it in idli.
- February 27, 2012 at 7:14:00 AM EST
- Pramod said...
Vani Madam,
Your post on Hurali Idly was truly inspiring, I made it today. It was so nice that the wife and kids refused to believe that it was horse gram and no urad dhal was used. I must tell you, a very nice companion to this is the Nati Koli Saaru ( prepared previous night). Recipe for the same is found here..(http://food.sulekha.com/mysore-style-chicken-curry-id4022-33711-recipe.htm)
- August 5, 2012 at 12:48:00 AM EDT


text:
Top Definition
Acronym for Designated Awesome Wickedly Godly Guy
Dude, Dawgg just completely owned that kid
af Joe Soutter 25. maj 2008
Trending Now
Can you define these popular missing words?
Usually used in "The Ghetto" or "Harlem". Slang word for close friend or accomplice. Found in ebonics, this word is sometimes used as code to confuse white policemen.
Yo dawgg, I was just riding my 'hogg' when i thought of a 50 cent song about sockggz...
af Johnsonforaface 25. februar 2004


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Click the link for daily surf reports presented by Nichols Surf Shop, 411 Flagler Ave., New Smyrna Beach
NSBNEWS.net video by Sera Frederick.
The video was shot by NSBNEWS.net during the 2009 inaugural Christmas on Canal, which featured a towering and beautiful artificial Christmas tree, complete with lights. The courtesy photo shows Hedda the Heron, the mascot for Historic Canal Street Merchants Association, with calendars for sale with the mascot's likeness to help pay for local activities. The tree lighting starts at 6 p.m. and NSBNEWS.net will have full coverage of all of the festivities.
Editor's note: The following story was submitted by contributing writer Dale Smith:
NEW SMYRNA BEACH – The holiday season kicks off in earnest Friday as Canal Street transforms itself into the second annual Christmas on Canal Street extravaganza: Five days of activities the whole family will enjoy have been designed to bring people downtown for entertainment, shopping and dining. Event organizers say they've combined Friday's Christmas caroling with Spark the Spirit night in hopes of attracting more people downtown.
Please click attachment to read NSB police patrols report:
Miguel Angel Frank departed this life on earth from Halifax Health Hospice of Volusia/Flagler, Edgewater Care Center, Edgewater, on October 4, 2011, and ascended into Heaven to join his beloved Cuban Family.


text:
VALENCIA, Pa. - The pro shop at the Ice Connection is unusually crowded for a Tuesday morning. The cause is easily explained - East Palestine native and New York Rangers forward J.T. Miller is signing autographs for a throng of young boys who - if their hard work plays off - may end up playing in the NNL one day. About halfway through the session, however, a young man, much larger than anyone else in line, bursts out of the pro shop with a huge grin on his face.
"I've finally gotten his autograph!" Matt Miller shouts to nobody in particular, waving a signed picture of his older brother in the air.
Despite frequently colliding on the ice, the Millers have kept their sense of humor off it.
With dozens of future NHL hopefuls watching, J.T. and Matt Miller held a workout in Valencia, a small borough about 35 miles north of Pittsburgh. While the drills were intense and often wowed the spectators, the Millers keep the mood light with jokes and cheers whenever somebody scored a goal.
"Thursday is the battle day," J.T. says. "Today was more about skills. When we're doing battles, it gets a lot more competitive. It all becomes pure ego at that point."
J.T. Miller, as the Ice Connection advertising flyers will inform you, is the 2011 first-round draft choice of the Rangers. At just 20 years old, J.T. has already seen action in 26 NHL games, scoring two goals and two assists. He has become an icon for the local youth hockey scene, a testament of how anyone can reach the big stage no matter where they're from.
"We're the first to play hockey from East Palestine, I think," J.T. says of he and his brother. "My dad saw the Pens win the cup in '92, before I was born. He wanted me to play, so I played. The closest rink to us was in Pittsburgh so we travelled all around. They sacrificed so much for us."
While it's difficult to match a blossoming NHL career, Matt has taken it all in stride. At 17, Matt is playing with the Youngstown Phantoms of the United States Hockey League this season, just as his older brother once did with the USA Developmental Team in Ann Arbor, Mich. While their brotherly rivalry is often amicable, the competition between the two on the ice seems to aid the younger brother greatly.
"There's definitely a higher tempo," Matt said. "It helps me get ready to come into the lower level games. I mean, he plays in the NHL so obviously he can teach me a few things."
There's not likely to be a better mentor than J.T., who has been watching Matt skate as long as he can remember. On Tuesday, J.T. was the leader for drills, often manuevering past orange cones and blasting shots toward fellow Pittsburgh resident and Michigan State goalie Jake Hildebrand. When Matt's development became a topic of discussion, J.T. offered high praise.
"He's a late bloomer," J.T. said. "When he was younger he didn't really have the tools or the size. Now it's just a matter of time."
The two brothers already have a bond that most professional athletes rarely attain - it's not often that siblings train on such a high level together. By practicing on the same ice for years, the Millers have adopted an unusually close working relationship that benefits both of them.
"We never got to play on the same team," J.T. says, "but we always know what to expect. The teammate chemistry is a bit different than this. It's become a hockey sense."
It helps that J.T. has been in Matt's shoes before. Unlike in Canada or parts of the Northeast, ice rinks can be difficult to find in the Midwest, specifically in the summer. The Phantoms have allowed both brothers to realize the amount of dedication and effort it takes just to get a shot at the NHL.
"I've been doing online school," Matt says. "I lived up in Michigan last year and it was easier with my schedule. With the Phantoms I get to work on fundamentals and conditioning. We get to travel and meet new people and it becomes part of a family."
While the journey to the NHL is certainly a difficult one, many young athletes have decided to lace up and skate toward that goal. Evidenced by the hordes of young players who lined up for autographs with J.T., hockey is as popular as ever in the Ohio Valley, something that both Millers have noticed and encourage.
"My draft year was really huge," J.T. says. "We had a lot of guys who went off to play everywhere. I think all the attention really helps. I think seeing the Pens win the cup really helps players push themselves even more. All these kids want to do is keep getting better and better."


text: 12 Christmas Duets by Kenneth Baird is a delightful collection of intermediate to advanced level duets based on traditional Christmas carols. They are challenging at times (not for beginners) without being overwhelming; at times joyful, at times uplifting, at times serene. In addition to being a great resource for teachers to play at lessons with their students, these duets are suitable for informal Christmas performances and many are appropriate for church services. Included are: Away in a Manger; Deck the Halls; Hark! The Herald Angels Sing; Joy to the World; O Come All Ye Faithful; O Come, O Come Emmanuel; O Holy Night; O Little Town of Bethlehem; Silent Night; The Wassail Song; We Wish You a Merry Christmas and What Child is This?


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quote SmurfNah, cause about 4/5 of those people would be like "um, wat?"I think it would be better with him just walking up and saying; You lost the game.
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Kenneth W. Leighty, the Altoona man who remains a suspect in the disappearance of his daughter-in-law 14 years ago, has asked that his bail on other charges be lowered so he can get out of jail.
Leighty, 65, was placed in the Blair County Prison on April 20 when investigators came to his home at 1225 19th Ave. to question him about a statement he made to his son - the husband of the missing Sherry Leighty - saying she was buried on a large plot of land he owns in Huntingdon County.
He allegedly resisted police and was charged with aggravated assault, simple assault and harassment. His bail is $250,000 cash.
Meanwhile, state police have been scouring the land in Huntingdon County, attempting to find evidence of the young mother.
Just Tuesday night it was revealed that bones and clothing were recovered from the 150-acre Huntingdon County site, but so far Huntingdon District Attorney George Zanic is not commenting.
Blair County Assistant Public Defender Edward J. Ferguson now has filed a petition in the Office of the Prothonotary and Clerk of Courts of Blair County asking that a judge order Leighty's bail reduced to "unsecured," meaning he will not have to put up any money to gain his release pending trial on the assault charges.
Judge Elizabeth Doyle of Blair County scheduled a hearing on the bail question for May 17. The hearing will be before President Judge Jolene G. Kopriva.
Ferguson said bail in the Leighty case was set in such a manner as to penalize him and to give Huntingdon County authorities time to investigate the alleged homicide.
Kenneth Leighty, Ferguson said, does not have the financial resources to post $250,000 cash and is not a flight risk. Leighty has no criminal record, and Ferguson said Leighty intended to appear at all court proceedings and to comply with whatever bail conditions the court imposes.
Mirror Staff Writer Phil Ray is at 946-7468.