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The fight over Betsy DeVos' nomination to be education secretary — just barely approved by the Senate with the tiebreaking help of Vice President Mike Pence — has, in the public debate, been mostly a fight over K-12 education policy.
I find the intensity of feeling on both sides about DeVos' views on K-12 a little baffling, given the limited role the federal government plays in K-12 education and the apparently similar results studies have shown are produced by traditional public and charter schools. Contrary to the hopes of her fans and the fears of her detractors, this is not an area where DeVos is likely to have a large effect on education outcomes.
When I worry about the Trump-DeVos Education Department, I worry about higher education, an area where the federal government plays a much larger policy role than in K-12.
By making rules about what higher-education institutions can rely on federal grants and loans to pay their students' tuition, the department plays an important role in ensuring that students and taxpayers get good value for their money spent on higher education. The Obama administration has been aggressive in this area, including forcing the closure of some scammy for-profit colleges.
President Donald Trump, of course, is the ex-proprietor of a scammy for-profit "university." One of his close advisers on education, Jerry Falwell Jr., is the president of a large, low-quality, nonprofit university with a major online component.
Given these associations, I do not have great confidence in Trump's Education Department to focus on ensuring that colleges deliver value in exchange for tuition. Failing to do so would be costly both to students and to the taxpayers, who would be on the hook for grants and defaulted loans to students.
This isn't an area where I think DeVos is likely to have especially nefarious instincts — but her hearing also did not generate confidence that it is an area where she would have especially good instincts either.
I will be watching her Education Department mostly to see whether it allows higher-education scams to run rampant.
This column does not necessarily reflect the opinion of Business Insider.


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File #: File No:

subset: commoncrawl


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Submitted by Bruce on Sun, 10/20/2013 - 21:27
Where other companies choose piping materials that are cheap or easy to produce, we select the material that works best. Where others continue using old and ineffective joining methods, we pioneer new and better ways. Where some might say "That's good enough," we say, "Let's find a way to make it even better." Not only do we provide high performance, long-lasting, and environmentally friendly pipes and fittings, we also have more efficient systems for potable water, radiant heating and cooling, heating and cooling distribution, industrial applications, water conservation, and fire sprinkler systems. We believe that the most sustainable products are also the ones that last the longest and have the lowest impact during operation.
BIM Page:


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TWO top officials were among a 300-strong crowd who watched Town's Academy lose 2-1 to Manchester United at Emley on Saturday.
Former Town manager Mick Buxton, who is now the FA's Academy monitor in this region, and Harry Wilson, who fills a similar role for the Football League, ran the rule over Gerry Murphy's matchday set-up.
All looked to be going well for coach John Dungworth's Under 19 side - featuring only three third-year scholars - when Matthew Young fired them ahead after 51 minutes at a pristine-looking Welfare Ground.
That seemed to inspire United, however, and Eddie Johnson headed them level only three minutes later.
United's win was sealed by Sylan Blake on 68 minutes, with a lovely side-foot finish after a flowing move.
Town, who have sealed a new matchday sponsorship deal with I.T.S. Turbos of Drighlington, just couldn't force an equaliser.
Among the crowd was goalkeeper Andy Jeffrey, who was on the bench 16 times for Town last season before being released from his non-contract terms during the club's financial crisis.
He is currently back at college and without a club.
Town Under 19s host Derby County in the next Academy game on Saturday.
Match Report


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"I'm running away from home," they declared.
"Well, people don't just run away without reason. Otherwise, they'd walk," I replied.
They puckered their lips in irritation. They drew their knees up. "It was boring there… and I didn't quite like the people," they said after a moment's honest thought. "I suppose I only stumbled upon this place by accident and I sort of like it. It's very empty in here–almost peaceful."
"I like to call it The Canvas," I said quietly after a moment of reflection. I took a good look around at the blank space that offered so much untapped potential. They were right. It was empty. But its emptiness was, in a way, its greatest deception. The inner clarity that a crystal sphere possessed was a similar lie. And the vacancy of an old man's cloudy eyes, too, was fools' bate.
I turned to my visitor then and met their eyes. "I could make something," I said.
"Like what?" they wondered aloud with a critical look around.
"Like… I could tell you a story and paint something. Anything. Or take a picture–maybe even share a moving picture with you!"
"Here? But it's peaceful here! Why ruin something already so perfect?"
I gave them a broad smile. "Just wait and see. You might prefer what I have to show you–what others have to offer you. This place… The Canvas... could become like a home to you–to others too. Like a nest for bored and restless souls."
"A nest, huh?" They mulled the notion over for a good minute before shrugging. "Anything's better than home right now… But if things get weird, I'm going back!" they said in firm finality.
I just smiled. "You're always welcome to go home and come back any time… even if things get weird."


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HOK-Designed Eskenazi Health Campus Is Among World's Most Sustainable New Hospital Projects
Eskenazi Health's main campus in Indianapolis, which includes the Sidney & Lois Eskenazi Hospital, has become one of the largest LEED Gold healthcare campuses in the world and the first in Indiana.
Eskenazi Health partnered with a team led by HOK on the design of the campus, which includes six buildings and a parking garage on 37 acres at the western end of the Indiana University-Purdue University Indianapolis (IUPUI) campus.
The LEED Gold certification for the 1.3-million-sq.-ft. campus encompasses several different building types, including an inpatient bed hospital, an ambulatory care center with a sky farm on the roof, an open-air outdoor plaza called "The Commonground," faculty office building, pavilion restaurant, central utility plant and central boiler plant.
"Given the diversity of buildings on the Eskenazi Health campus, we were delighted that the team led by HOK was able to exceed our initial goal of LEED Silver to achieve LEED Gold certification," said Dr. Lisa Harris, CEO of Eskenazi Health. "Our focus at Eskenazi Health is on promoting health in all its dimensions. Our priority remains the overall health of our community in the broadest possible terms. We view this environmentally friendly hospital campus as a statement of our commitment to those we serve now as well as our commitment to generations to come."
"We are honored to have contributed to Eskenazi Health's commitment to creating a healthier, more energy-efficient environment in every aspect of its operations," said Paul Strohm, AIA, ACHA, LEED AP BD+C, director of Healthcare at HOK. "Their dedication to sustainability enabled the team to collaborate to create an innovative, high-performance design with four distinctly different building types: healthcare, office, restaurant, and central utility and central boiler plants."
Sustainable design innovations that helped the Eskenazi Health campus earn LEED Gold certification include:
- An extensive health education program for patients, staff and community members.
- A 35,000-sq.-ft. green roof, including a sky farm that grows 2,000 pounds of produce per year that is served in the hospital and in a campus restaurant.
- An on-site farmer's market.
- Six healing gardens.
- A rainwater harvesting system that supplies 100 percent of the campus' irrigation needs.
- On-site stormwater treatment and infiltration systems that support the adjacent White River ecosystem, decreasing runoff by 40 percent compared to pre-project conditions.
- Low-flow fixtures that reduce potable water use by 40 percent.
- Recycling 80 percent of construction waste, with 48 percent of materials procured from regional suppliers.
- A parking program for low-emitting and fuel-efficient vehicles and for those who choose to carpool to work.
The hospital includes an art program that provides connections with nature. Eskenazi Health also is collaborating with the city of Indianapolis to develop eroded and refuse-filled land into a future public park.
The Eskenazi Health campus, which opened in December 2013, includes a 315-inpatient bed hospital with 19 operating rooms and four interventional labs, 12 labor and delivery rooms, a 100-bed treatment room emergency department with a 16-bed clinical decision unit and more than 200 ambulatory clinical exam rooms. It is one of only two adult Level I trauma centers in the state, and the only adult burn center serving Central and Southern Indiana. The hospital links functionally and operationally to the adjoining Outpatient Care Center, which houses primary and specialty care. The campus also includes a 2,700-car parking garage that serves as the canvas for the "May/September" public art installation.
HOK served as the executive architect and sustainability consultant, with Platinum Earth, Blackburn Architects and Urban Design contributing to the LEED certification management. Syska Hennessey Group, Inc. served as executive engineer while RATIO Architects, Inc. was the architect of record and BSA LifeStructures served as engineer for the Ambulatory Care Building. LAND Collective, Olin Partnership, Context Design and Ninebark Landscaping, Inc. joined HOK on the landscape architecture team.


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By Fred Green
As the white and gray form – of spruce, fir and juniper – entered the water from Parrott's slip, they saw the lie and the new scall'n oar.
"She sits good in the water!" someone said.
Did anyone see the roughness of hull, a cracked timber, oakum on the inside? What about the strange looking starn knees? Did anyone wonder if t'was a motor boat or maybe a punt? Did some brave soul dare say, "She'll be cranky, b'y?"
Well, she's light in the water and the wood's awful dry from years indoors being shaped to tunes of, "she'll be finished, take your time, what's that part called? And how do you spell it?"
Yes, all these things and more were noticed if not spoken. But few saw the shards in the axe from cuttin' knees close to the rocks below; the strange tracks made in early morning March snow crust as 20-foot plank sticks were dragged by hand out the haulin' path.
What neighbour missed the trees: selected, cut and hauled from a friend's woodlot? Only two of them saw the plank break before the last galvanize was driven: plank that is now a gunnel. See the spoiled timber floor in the corner? 'Tis now the splits for next winter.
Who heard the curator say, "Leave all the chips and shavings on the floor," (inside, 1998). And "Don't throw chips on the ground!" (outside 2003)? But he was usually heeded. Good thing, too; or she wouldn't be launched today.
Is it significant to anyone else that the midship bend, placed at the middle of the keel, was holding down the spot of the builder's desk in grade 10?
You should know that some of her parts moved from Stephenville to Clarksville before ending up in the upper room. Did anyone count how many tourists commented and questioned – some innocently, some with knowledge – as she came under public scrutiny?
Who saw the four generations? One gave the hull its shape; the next guided the builder; then the builder and his two sons aboard at the launch. Anyone see the boyhood dream; the growing respect for the builders before? What about the love and skills shared as dad was there for every day and every stage of a dream come true? Oh, the strange talk between them of breasthook, apron and tawts.
Did his doctor know they put in the shoots – in the ICU?
See the sheer, put on with his eye! "How much should I take off here?"
"Whatever you t'inks. 'Til it looks right."
There's no such mark on the builder's rule. Now the other marks are in the heart, and in the soul: through a glimpse of ways coming not from books. Their mind's eye held many shapes, know-how and the right from wrong. From being around helping forefathers build.
What was private and personal became a public event embraced by a special place once called Scilly Cove. The builder is grateful for the rewards – a boat, yes, but far more. A dream fulfilled and a lasting memory. 'Tis surely true that the trip holds more stores than the goal attained or the place found.


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Little Venice on Regents Canal Regents Canal is the busiest section of canal in London
Cyclists are being asked to adopt Victorian-style manners and make sure they "ting ting" when approaching pedestrians on London's busy towpaths.
British Waterways said complaints from pedestrians and cyclists sharing Regents Canal led it to issue a guide from etiquette authority Debrett's.
The guide asks walkers to give way to cyclists and riders are reminded to "smile" and say "a polite thank you".
A Victorian-themed event marked the launch of the guide at Regents Canal.
The British Waterways, which first launched Two Tings campaign in 2007, said its research showed people consider Victorian age as the most polite era and it decided to prepare a code of conduct and had now decided to call in Debrett's.
'Be considerate'
London has about 100 miles of canals and Regents Canal, which runs from Paddington in the west to Limehouse in the east, is the busiest walkway in the capital.
During peak times about 400 cyclists and an equal number of pedestrians use the route every hour, British Waterways' towpath ranger Joseph Young said.
"We are seeing an increase in the number of speeding cyclists, who seem to forget, or aren't aware, that pedestrians do have right of way," he said.
"Sometimes cyclists can forget how fast and threatening they can be if they are passing you at speed. The Two Tings campaign asking cyclists to slow down, and pedestrians to listen out for bikes," he added.
The etiquette guide tells cyclists and pedestrians to be "considerate to each other" and also asks people "never drop any litter" and urges dog walkers to "clean up after their dog".
Jo Bryant of Debrett's said: "We hope that these top tips will be a gentle reminder to towpath users, and encourage a return to more polite and amicable behaviour along the waterways."
via BBC News – Debrett's cyclist etiquette guide for London canals.


text:
KVC (UK) Ltd design, manufacture and supply a range of metal seated ball valves from 1" up to 48", CL150 – CL2500 and API 5000 & 10000. The KVC metal seated valve is manufactured to the highest standard and quality to ensure a precision engineered product is supplied to our customer.
Metal seated valves are available in various sealing arrangements from o-rings or enerseals to full grafoil construction to suit the required environment and operating conditions, including high temperature and abrasive service. The ball and seat sealing faces are coated with a hard surface material and individually paired and lapped together for efficient sealing.


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HappyMetrix let's you create data dashboards with all your key metrics. In just a few simple steps you can create a stunning dashboard by adding a couple of easy-to-use data widgets. Or create a dashboard instantly by selecting one of our dashboard templates.
You can connect with a number of essential platforms like Google Analytics, Facebook, Mailchimp and others. The number of connected services is an ever growing list. And importing or connecting your own data is just as easy.
HappyMetrix makes it easy to share your data with others. You can share your dashboards with your colleagues over the web, or export your dashboard as a PDF and report back to your clients.
Working on a presentation? Our stunning widgets can also be exported independently to be used as images in all your projects. And if you feel like bragging, you can also share your key metrics on social media.
Want to monitor your key metrics all day? Presentation mode makes all your dashboards pop out on a larger display. Having a live dashboard up on the wall at the office is a great way to stay on top of your business.
In presentation mode your live dashboards refresh automatically, and if you have multiple dashboards you have to option to rotate between them.
Bring your dashboards with you wherever you go. HappyMetrix dashboards look great on tablet and it's a great way to check how your business is performing, even on the go. HappyMetrix for tablets is currently available for iOS and Android.
Away from the office without your devices? No problem. Just log on at cloud.happymetrix.com on any computer with a web browser and all your dashboards will be loaded instantly.


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Your shopping cart is empty!
Craft projects do wonders with toddlers' hand to eye coordination and cognitive development. All Tots Treasures has handpicked a series of art and craft games and supplies for kids that will help with their overall development and allow them to have fun at the same time.


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"Never does Nature say one thing and Wisdom another." – Juvenal
Philosophy means "love of wisdom," but for us it's about loving people and Life first. Our mission is intelligent nourishment: spreading consciousness and nourishing all Life through delicious food designed for human consumption.
We accomplish this through the open-source regenerative business model we have pioneered called "Nourishing the Roots." In this model, a food product company can use a portion of its profits to support or establish an organic farm or orchard that will provide ingredients for the food product company in a mutually beneficial fashion.
The model works like this: the food product company creates demand and starts generating revenue, revenue is used to support or establish an ecological agriculture project that wholesomely grows ingredients for the company, and the cycle is established: land feeding company feeding people feeding land.
Excess revenues can be used to support and establish more regenerative orchards. In this way, we will create self-seeding systems of abundance that nourish Life at every level in every step of the production and consumption processes.
What is a regenerative orchard?
Normally, almond orchards are large monocultures designed to create food for one species (humans) at the expense of all others. Only almond trees are grown; concerted efforts are made to keep out other plant, bacterial, fungal, insect, and animal life.
In the largest human-induced pollination event on the planet, bees are trucked in from all over the country and forced to pollinate crop after crop, while having their honey stolen in exchange for sugar-water or high fructose corn syrup. California grows 85% of the world's almonds in this fashion, which is detrimental to the health of the trees, bees, humans, and ecosystems involved.
That's not the way that nature works. Nature works in relationships, interconnections, biodiversity, and cooperation. Permaculture design is about mimicking nature to create abundant human systems that are intertwined with abundant natural systems. Permaculture establishes polycultures that nourish all of Life, human and non-human included. Unfortunately, we don't know of any examples of nut orchards that are designed according to Permaculture. That's where Almondia comes in.
Almondia: the world's first regenerative almond orchard
Almondia will support the thriving of microorganisms, insects, plants, animals, humans, and ecosystems by going beyond the monoculture mindset towards a paradigm of fractal flourishing. Fractal flourishing is our term for a state where everything from the quantum to the cosmic level flourishes and in so doing enables the flourishing of everything else in symbiotic harmony.
In Almondia, the primary crop will be almonds and walnuts, which will be the main economic engines for the project because they grow well in the climate of the Central Valley of California, but there will also be food and medicine growing between the trees for humans and other animals. Microorganisms, insects (especially bees!), and other plants will also receive the best possible nourishment, being holistically integrated into the landscape.
Efficient Microorganisms (EM) will help the soil thrive and will be sprayed on the trees to deter pests. All of this activity will create fresh air, clean water, and healthy soil in the ecosystem. Humans will cooperatively work with each other and the rest of Life to enable fractal flourishing! If you would like to help us make this happen, please contact us or consider funding us through Credibles.
Nourishing the Roots – an open-source business model
Almondia is an example of using a food product company to create real change in the supply chain of our food system. This could easily be done with the the root agricultural ingredients of any food product. If we nourish the roots of our food products, we nourish the roots of our lifeways. We are offering this model to be used by any other food business to take control of its own supply chain while creating a more vibrant and resilient ecosystem and food system.
This company is testing our hypothesis that consumerism can make the world a better place, rather than the story we're used to telling and hearing: that it destroys the world. We believe that we can harness the power of the market to nourish all Life. We believe that dollars can become seeds, trees, food, health, and Life.
In a similar fashion to an almond tree, in our business we engage in relationships and activities that nourish us and all the things with which we interact. We spread our abundant seed butters while receiving the abundance of everything else.
In the processes of this energetic exchange, we put energy into producing a beautiful and flourishing seed (Almondia), which in turn will nourish the company and help propagate its life-giving model. Almondias will blossom like an almond tree in spring, enabling the thriving of humans and the rest of nature in each location.
Nourishing the Roots regenerative business model by Tim Richards is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License


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This is regarded to be a humane method.
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Cape Henlopen Senior Center Website Project
Project Overview
Cape Henlopen Senior Center wanted a basic website that was easy for the target audience to read and navigate. We used colors chosen by the client, and their existing logo to create a template website using the Joomla! Content Management System.
We used the GCalendar component to create calendars for several different categories of events offered at the Cape Henlopen Senior Center. Events are clickable, and open pop up window featuring complete description and details of each event. The client can add and update events simply by entering in into a Google Calendar.
The Contact Page features an interactive Google map with driving directions to the Senior Center to and from any address entered. Fine Line also created a simple contact form, allowing the online audience to contact the Senior Center easily and conveniently. Contact forms are tested to ensure they are working properly.


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Product Description
This Flow Series Halo Kit is for our customers that really like to show off! Featuring full color functionality like our Colorwerkz kits, along with a solid white output like our RGBW kits, these ALSO feature over 200+ Sequential/Chase Modes + Functions, you'll have the ability to transform your headlights with the click of a button, the available color combinations are virtually endless, you'll definitely make your competition think twice before pulling up next to you!
Order Includes:
- (2) Polaris RZR Pre-Built Flow Series Headlights
Vehicle Compatibility:
This kit fits the following vehicles:
- Polaris RZR 900S
- Polaris RZR 1000S
- Polaris RZR 1000XP
We ship worldwide! We also offer expedited shipping if you're in a rush! If you need a custom shipping quote please contact us at firstname.lastname@example.com (Attn: Shipping).
Install Help:
For help with installation please reach out to us through our contact page,
any other questions or concerns please direct to (248) 974-5725 | Mon-Fri 9am-5pm.
This particular product is covered under a (1) Year Warranty.
Customer Reviews
Pre-Built Polaris RZR Flow Series Headlights


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Saturday, September 15, 2007
Library Limbo
Study-time cycles in Uris Library with 1920's style animation. A student project made at Cornell University in Lynn Tomlinson's Film 325 summer course
Posted by Nancy Dowd at 10:04 PM
Looking to find the best videos about libraries on the web? They are here. Submit your favorite or rate ours!


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Healing ourselves we change the world.
Mother Hulda, the presence of the wise woman in the Elder tree who,
through her gifts of her flower blossoms, fruits, bark, leaves and roots
provides support for our renewed health..
We might also find healing by giving up our worries to her protection,
binding our difficulties to her branches or burying
that is no longer useful alongside her roots.
In that spirit I want to offer you a welcoming and supportive healing space.
One where to heal your story and to fully let go of adversity you can allow youself to flourish and prosper.
You might have questions like:
Who am I?
What do I want and how can I change my life?
Who can I find out what needs healing
How do I find the willingness do let go?
How can I embrace my strengths, my dreams, my unique calling?


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Dignus 510
Stone sinks Dignus 510
Dignus 510 is quite minimalistic in its form. It is round with straight sidewalls lightly angled. It is very deep (22cm) and that way very comfortable to use. Diameter equals 40cm. It is always made of one piece of stone. We never use glue, nor putty, nor paint. Thanks to that sink never absorb watter and never leak.It is handmade by our most qualified craftsmen in Indonesia. Manually drained and polished it is smooth and shiny. There are variety of available stones. We use cream, grey and yellow marble, green sandstone, black andesit and yellow honey onyx. Each single sink differ a bit from others thanks to mineral veins that often appear. Each stone sink is unique and unrepeatable.
Dignus 510 stone washbasin is often to be seen in modern designs bathrooms e.g. luxury restaurants and hotels.
Technical specification:
|Manufactured by
|Marble, Andesite
|40x40x22 cm
|about 30 kg
|Marble sinks, Onyx sinks & andesite sinks
Available Stones:
Yellow Onyx
Cream Marble
Black Marble
Yellow Marble
Grey Marble
Green Sandstone


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Liberal nut job Tweets Sheriff Clarke's home address calling for left-wing attacks against him; reported by Freedom Daily.
These left-wing extremist nutjobs would have gone stark raving MAD if the militias has done anything near what they have done in recent weeks, yet they are the ones calling conservatives extremists? Remember when former DHS Secretary Janet Reno told us the greatest danger we have to national security was "right wing extremists?" We so-called "right wing extremists" didn't go around shooting people who we didn't agree with. So who are the real BIGOTS? That's a no-brainer. Left-wing Marxist extremists, aka; the Democrat Party are the greatest threat to national security.
So what do you do? We say fight fire with fire. It doesn't take a huge budget to be able to find out anything you want about ANYONE you want these days thanks to the Worldwide Web.
We propose that anytime a left-wing loon decides to take it upon themselves to "dox" a public official and call out their Libtard extremists to inflict violence upon them to return the favor. Find out the home address of the offending party and post it on all of your conservative Twitter accounts and Facebook groups and Pages.
For those of you who aren't up on the term "dox," here is what it means:
"Searching for and publishing private or identifying information about (a particular individual) on the Internet, typically with malicious intent." "hackers and online vigilantes routinely dox both public and private figures"
How does the old saying go? "Turnabout is fair play?" (Every dog has his day.)
Liberals better get used to the idea, because the militias are watching them, and they really don't want to screw with the militias.
Militias Forming – USA Preparing Anti Trump Election Riots
Please SHARE this post to all of your social networking websites using the links below. We need to put the left-wing extremists on notice that we are THROUGH putting up with their childish antics!
Login below on our DISQUS comments using your Facebook profile or sign-up with a DISQUS profile to leave a comment or join the conversation!


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Find financing options with your business needs in mind.
We're business people too. We'll help you find the right options and the right loans to help your business succeed.
Business Credit Card
Business Visa® rewards credit card is a convenient way to pay for everyday business expenses.
Business Lending
Let us help your company achieve its goals.
Business Lending Team
Our business relationship officers are commercial lending experts ready to assist you every step of the way.


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Welcome to the Lincoln Air Force Junior ROTC website. Each day, we are updating our website, so if you don't see the information you are looking for, please check back or contact us directly.
Below you will find a flyer explaining our program as well as a basic information sheet with additional information:
Basic Information Sheet for Lincoln Air Force Junior ROTC
Lt Col (Ret.) Terence Plumb (email@example.com)
MSgt (Ret.) Trent Woodruff (email@example.com)
"The information and opinions contained in this internet site do not reflect the official policy or position of the United States Government, United States Air Force, or Jeanne M. Holm Center of Officer Accessions and Citizen Development."


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Senate Backs Pullout Proposal
Wednesday, March 28, 2007
Senate Democrats scored a surprise victory yesterday in their bid to force President Bush to end the Iraq war, turning back a Republican amendment that would have struck a troop withdrawal plan from emergency military funding legislation.
The defection of a prominent Republican war critic, Sen. Chuck Hagel of Nebraska, sealed the Democrats' win. Hagel, who opposed identical withdrawal language two weeks ago, walked onto the Senate floor an hour before the late-afternoon vote and announced that he would "not support sustaining a flawed and failing policy," adding: "It's now time for the Congress to step forward and establish responsible boundaries and conditions for our continued military involvement in Iraq."
Democratic leaders think the 50 to 48 victory greatly strengthens their negotiating position as they prepare to face down a White House that yesterday reiterated its threat of a presidential veto. The Senate vote was also the first time since Democrats took control of Congress in January that a majority of lawmakers have supported binding legislation to bring U.S. troops home.
The Senate withdrawal provision, which sets a March 31, 2008, target for ending U.S. combat operations, is tucked into a $122 billion package to fund operations in Iraq and Afghanistan, a must-pass bill that Democrats view as their best shot at forcing Bush to change direction. The withdrawal language was nearly identical to that of a Senate resolution rejected 50 to 48 two weeks ago.
Top Democrats in the House and the Senate had been uncertain about the outcome of the vote when they convened for a joint leadership meeting yesterday morning. They were convinced that defeat of the Senate's proposed timeline would force negotiators to soften the House language, which sets a firm deadline of Aug. 31, 2008, for the removal of U.S. combat forces. But they concluded that a Democratic victory would give them no reason to compromise, according to House Democratic leadership aides.
Speaking to reporters, House Majority Leader Steny H. Hoyer (D-Md.) was conciliatory, but only to a point: "We ought to reach out to the president and say, 'Mr. President, this is not a unilateral government. It is a separation of powers, and the Congress of the United States . . . has taken some action. You obviously disagree with that. Where are the areas of compromise?' "
Senate Majority Leader Harry M. Reid (Nev.) said he was skeptical about proceeding too quickly. "Of course, we should reach out to the White House, and I'm happy to do that," he said. But, he added: "They have been very uncooperative to this point. Hopefully, they will cooperate with us." Referring to the president, he said, "I would like to have a bill that he wouldn't veto."
Senate GOP leaders remain confident that Bush will ultimately prevail. "I expect the president to get the money for the troops, to get this bill in large measure like he wants it," predicted Senate Minority Leader Mitch McConnell (Ky.). "It may take two tries to get there, but I think that's very likely going to be the final outcome."
But Democrats are just as convinced that they have the momentum on the issue. "This is not one battle. It's a long-term campaign," said Sen. Charles E. Schumer (N.Y.). "Every time we have a vote like this, it ratchets up the pressure on the president and on many of those of his party."
Under the Senate bill, which is slated for a vote on final passage as early as today, certain U.S. troops would remain in Iraq after the March 31, 2008, target date in order to conduct counterterrorism training and security operations. But troop withdrawals would begin within four months of enactment.
The White House has strongly protested both the House and Senate bills, issuing a series of veto threats. "This bill assumes and forces the failure of the new strategy even before American commanders in the field are able to fully implement their plans," the administration said in a statement yesterday, referring to the Senate measure.
Democrats and Republicans largely remained united in the Senate vote, with only Hagel and Sen. Gordon Smith (Ore.) on the GOP side voting to preserve the withdrawal provision, and Sen. Mark Pryor (Ark.) the only Democrat to break ranks. Yet, on both sides, several senators remained undecided until the roll was called, and Vice President Cheney was on hand to break a tie in the case of a deadlock.
Sen. Olympia J. Snowe (R-Maine) said she was torn between her desire to send a strong message to the president that a change of course is needed and her uneasiness about wading into war policymaking. "Clearly it's frustrating," she said of the grim conditions in Iraq. "On the other hand, you don't want to telegraph to the enemy a moment in time" for leaving. Snowe wound up voting with her party.
Because troop funding is at stake, Republicans have decided to forgo maneuvers that could draw out the Senate debate or block final passage, tactics the GOP had used successfully in previous Iraq war showdowns. Some GOP senators even floated the idea of introducing the Iraq war legislation of Sen. Hillary Rodham Clinton (D-N.Y.) as an amendment to the spending bill, in a bid to make political mischief. The Clinton proposal would cap troop levels, start a phased withdrawal and cut off Iraqi security funding under some circumstances, but so far has attracted no co-sponsors.
Reid said final negotiations between the House and the Senate will take place after the spring recess. Despite signs that Democrats are slowly building support for their position, they are still nowhere close to achieving the two-thirds House and Senate majorities that would be necessary to override a Bush veto.
Staff writer Jonathan Weisman contributed to this report.


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King Philip's War: The Praying Indians of Marlborough and the Summer of 1675On June 26, 2012, Paul
Brodeur, a Marlborough Historical Society Trustee and lifelong resident, presented the first part of a planned three-part talk. He gave the second in the series on February 25, 2013. The slides from the first two are below. When the third in the series is given--not yet scheduled, but likely next year--those slides will be added, too. (Paul's other presentations include Abolition, the War, the Bell and Artemas Ward Park.)
As Paul described Part I, "In the summer of 1675 King Philip was on the warpath and the colonials were getting very anxious. In the frontier town of Marlborough a series of events would set the tone for the war and shape policies toward the Indians throughout the country."
And in Part II, "As the combined colonial forces attempt to root out the Indian army of King Philip from central Massachusetts to the Connecticut River Valley, Marlborough becomes an important colonial army outpost on the frontier. It also becomes a target for attack, culminating in its destruction and abandonment in the spring of 1676."
The slides from the both presentations are below. To view the slides full screen, click on the icon at the right end of the grey border below each presentation.


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Head to the beach or pool in style with this customizable bag.
Bag features full lining, zippered top, inner pocket with zipper, and outside pocket with zipper. Bag measures 22″ x 8″ x 12″.
Embroidery options include: no embroidery, single initial, monogram, and full name. Select "no monogram" when ordering if you choose the "no embroidery", "single initial", or "full name" option. Provide name or letters in "personalization" section.
Monogram format as follows: firstinitial|LASTINITIAL|middleinitial
Due to the unique, custom nature of this item, all sales are final. Proof acknowledgment shows you approve all details sent for embroidery.
Production time is currently 3-4 weeks.


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Watch Emma Roberts, Dave Franco, and Casey Neistat Prank the Press
Source: www.youtube.com / Via: www.youtube.com
Remember Jennifer Lawrence pranking Smosh? Emma Roberts and Dave Franco are getting in on the press junket prankster racket, too — and the results are fantastically funny.
Roberts and Franco agreed to let YouTube genius Casey Neistat (who, last time we checked, was wakeboarding in a tux through Amsterdam) and his merry band of pranksters put an earpiece in each of their ears and feed them instructions. What do Neistat and co. make Roberts and Franco do? You'll have to watch the video to find out, but it involves the words "Mommy needs a drink."
While this isn't the only prank of its kind — there's a similar recurring bit on The Ellen Show that later got turned into ABC's Repeat After Me — it's one of the funniest examples of it I've seen. The interviewers, Clevver TV's own Erin Robinson and Joslyn Davis, are especially good sports about all the weirdness thrown their way, too.
Of course, Roberts and Franco aren't subjecting themselves to public humiliation just for fun. They're promoting the upcoming Lionsgate thriller movie Nerve, in which they both star. (Casey Neistat makes a cameo appearance, too!) Peep the trailer below, and check out Nerve in theaters July 27th.
Source: www.youtube.com / Via: www.youtube.com
What do you think? Were the interviewers great sports or what? Let us know in the comments below or @WhatsTrending on Twitter!


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Guidepost #3: Cultivate a Resilient Spirit - Part Two
Last Spring I wrote a 10-part series based on one of my very favorite books, "The Gifts of Imperfection" by Brene Brown. Each week for 10 weeks I shared one guidepost from the book, a few thoughts, plus a journal prompt. For an overview of each guidepost visit here.
Guidepost #3: Cultivate a Resilient Spirit – Let go of numbing and powerlessness
I have a feeling I'm going to say, "This is my favorite guidepost!" for the next 10 weeks but this is a really good one! When I write articles like how to lean into discomfort and about the cause of anxiety what I aim for are more ways to teach you (and me) how to cultivate resilience.
Next month's book selection, Taking the Leap by Pema Chodron, is about this too. Basically we have zero tolerance for uncertainty. Uncertainty comes in many forms and is different for each of us. Uncertainty is anything that makes you feel like the ground beneath you is being taken away or just gone. Like financial worries, heated conversations, intimacy, grief, even your relationship with food or your body. Uncertainty is the inability to predict a safe future.
No wonder it's hard to sit with! Resilience is being with the things that aren't certain and not running away or numbing from them. We all have our versions of numbing. Bad TV, Facebook, wine...numbing takes the edge off of the things that make us uncomfortable.
Numbing has become quite acceptable. Anything you do to not touch the immediacy of your life is deemed 'normal'. By cultivating resilience, slowly, you let go of your need to escape into the things that keep discomfort at bay. Resilience is the ability to feel fully and embrace it all.
To help you cultivate guidepost #3 I have a journal prompt for you:
If I embraced my fears...
Join me next Friday as we explore Guidepost #4: Cultivate Gratitude and Joy – Let go of scarcity and fear of the dark.
In the meantime bring this guidepost into practice by trying the journal prompt this week.
In light and love,


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Guided Math for 5th Grade Teachers
Your hands on math lessons planned out for you so you don't have to do the work!
5th Grade Guided Math by A Teacher's Wonderland
What is Guided Math? Guided Math is a method of teaching that allows the students to develop skills independently and through math centers along with teacher directed tasks. You will experience a difference in your math lessons when you use Guided Math. Your lessons will run smoothly, you will be able to differentiate your groups, allow students to successfully work independently and collaboratively with classmates to work on classwork or math games.
Each unit contains
- A lesson page with:
- Standards
- I can statement
- Materials
- Mini-Lesson
- Support for lowest students
- Support for low-medium/high-medium students
- Challenges for high students
- 2 Classwork pages
- An exit slip for each lesson (have to get your grades somehow!)
- 2 Homework Pages
- A unit assessment
- "The amount of detail in each lesson was amazing! This made my life a million times easier!"- Monica
- Words cannot express how amazing these units are! Thank you so much for sharing! The units are broken down beautifully with meaningful activities, and the assessments are great! These are worth the money! -Mrs. Wilkins
- Our book does not do a good job providing adequate practice for students and we have had to supplement a lot of our own stuff. This is exactly what we have been looking for! Thank you!! – Samantha
Who is this for?
Any 5th grade teacher that wants to step away from the text book and use hands on learning to engage their students on a daily basis.
Are you looking to get started with Guided Math but you don't know where to start? Check out our
How to Implement Guided Math in Ten Days Freebie!
*Click the picture to get your Freebie!*
Q. Will there be other 5th Grade Guided Math Units?
A. Unless other teachers need additional series, there will only be 11 units.
Q. Will there be other grades with your Guided Math series?
A. Yes, I am starting from the top and working my way down. I just started working on 4th grade Guided Math Units.
Q. Are you selling to schools?
A. Yes, in fact, I have sold to several schools to help supplement and support their curriculum.
Q. Does this really work?
A. Yes, with time and patience you can have a successful Guided Math Classroom.
My Story
Guided Math has been huge in my teaching career. When I switched schools, I looked very hard for 5th grade Guided Math Curriculum. I never found it. I decided to start creating centers and rotations to help myself throughout the school. Then one day I realized that if I was struggling finding 5th grade curriculum, other teachers were as well. Instead of just making stuff for myself, I decided to help other fifth grade teachers across America!


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Montana Landscaping Maintenance LLC
Season Of Clean Up & Many More
Residential Lawn Maintenance
Commercial Landscaping Maintenance
As the leading home improvement company for landscaping in Portland, OR, Montana Landscaping Maintenance LLC specializes...
Residential Lawn Maintenance
If you want to bring beauty to your landscape, trust Montana Landscaping Maintenance LLC to maintain your yard. We...
Welcome to Montana Landscaping Maintenance LLC
Montana Landscaping Maintenance LLC is a company specializing in residential and commercial lawn care services in the Portland, OR area. It is our mission to provide every customer with the highest level of service, quality, and value, along with distinctive products and designs. We provide a "Season Of Clean Up & Many More."
As outdoor living and landscaping experts, we create and maintain your outdoor environment, transforming your yard into your outdoor home. We believe that family and friends connect in extraordinary ways when their outdoor space is warm, inviting, and fun. Our crew pays close attention to details. We ask a lot of questions to get a solid idea of your needs and desires for your landscaping. In turn, our landscapers provide numerous options, and we do what we say we are going to do.
We know that some of the best memories for homeowners are of spending quality time with family and friends in the yard. So, we want to help you continue to make wonderful memories. We continue to ensure the longevity of our company by not only meeting expectations, but exceeding them as well. Our services include:
• Lawn Care
• Gardening
• Turf Removal
• Tree Maintenance
• Landscape Design
We have been in business for five years, and as the leading landscaping contractor, we make sure to complete your project on time and on budget. Take advantage of our free estimates! If you want to beautify your outdoor space, ask Montana Landscaping Maintenance LLC.
Montana Landscaping Maintenance LLC
8996 N Westanna Ave
Portland, OR 97203
(503) 490-5837
(971) 331-0276
(360) 843-9790


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Go Healthy Propolis 500mg is a broad spectrum natural remedy that supports the maintenance of strong immunity.
Propolis is collected from the buds of trees and flowers by bees and is used to seal and protect their hives from outside bugs. Propolis contains flavonoids which are thought to be responsible for its antimicrobial effects.
Good Health Colostrum Capsules uses the bovine colostrum of healthy pasture-fed New Zealand dairy cows. The colostrum is a rich and completely natural source of nutrients including protein, vitamins, minerals, and immune enhancing factors. It is especially high in immune factors including the valuable lgG antibodies to help strengthen the immune system and high in repair and growth factors to help speed up recovery times and help with the growth of cells, muscles, tissue, bone and cartilage. It is also highly nourishing for the body, helps with general wellbeing and even slows down the signs of aging.


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Nursing home safety: a review of the literature
Document Type
Peer Reviewed
Publication Date
NLM Title Abbreviation
Annu Rev Nurs Res
Journal/Book/Conference Title
Annual Review of Nursing Research
PubMed ID
DOI of Published Version
Start Page
End Page
The number of older persons in the United States is rapidly growing and, based on this growth projection, the number of consumers needing nursing home (NH) care will likely triple in the next 10 years. Although NHs have been bombarded and scrutinized about the care that they provide, the concept of safety (specifically, error prevention) remains at the margin of most quality improvement efforts. The purpose of this review is to explore what has recently been written (2000-2005) about the evolution of the NH as an organization focused on safety and the most critical clinical processes that must be closely monitored for a safe NH environment to occur. After a thorough review of both organizational and clinical NH literature, 30 organizational studies and 39 clinically based studies were reviewed. The review revealed that, organizationally, teamwork, communication, and leadership all were critical in resident and staff outcomes and clinically, assessment was an important missing process at critical points in the residents' care for prevention and timely treatment of potentially dangerous conditions. The value of the registered nurse (RN) in this setting was clear in the many assessment issues noted and the lack of RN guidance for adherence to recognized practice guidelines. To explicate the role of the RN, first, better outcome measures must be developed that are nurse sensitive. A second clear agenda for NH research is the explication of the role of leadership, particularly nursing leadership, to create an environment where open and accurate communication can be accomplished among all of the diverse NH roles. This will help all members of the team to identify care improvement opportunities. Finally, a new frontier for the NH setting is the use of technology and the need to harness the information that has set in the NH system for years. Information mastery for staff and leadership is a necessary aspect of the organization that must be developed to provide sound information for strategic and focused change to occur.
Aged, Communication, Forecasting, Geriatric Assessment, Health Facility Environment/organization & administration, Health Services Needs and Demand, Humans, Interprofessional Relations, Leadership, Medical Errors/nursing/prevention & control/statistics & numerical data, Nurse's Role, Nursing Assessment, Nursing Evaluation Research/organization & administration, Nursing Homes/organization & administration, Nursing Staff/organization & administration, Organizational Culture, Patient Care Team/organization & administration, Personnel Staffing and Scheduling/organization & administration, Population Growth, Practice Guidelines as Topic, Quality Indicators, Health Care/organization & administration, Safety Management/organization & administration, Total Quality Management/organization & administration, United States
Published Article/Book Citation
Annual Review of Nursing Research, 24: (2006) pp.179-215.


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Velocity Micro® Wins 17th PC Magazine Editors' Choice Award
Home/Office focused Vector™ Z25 lauded for its value, performance, and design
Richmond, VA — April 18, 2013 — Velocity Micro®, the premier builder of award winning enthusiast desktops, laptops, peripherals, and Cruz™ tablets has won another PC Magazine Editors' Choice award, their 17th from the highly regarded publication alone. The newly decorated desktop, the Vector™ Z25, is designed for home and office use but also handles enthusiast tasks such as video editing and gaming dutifully, according to the magazine. The Vector™ Z25 is available today on VelocityMicro.com starting at $749, with systems matching the reviewed configuration available for $999.
"Every award that we win is special, representing enormous amounts of hard work by everyone involved, from our product development team, to our engineers, down to our hardware and software partners," said Randy Copeland, President and CEO of Velocity Micro. "But PC Magazine's Editors' Choice award is one of the most prestigious in our industry and seventeen of these awards is a major accomplishment. This is truly a team effort, and one that I'm very proud to have been a part of."
The editors at PC Magazine praised the Vector™ Z25 for its robust performance, ample expansion space, and incredible value. Priced at just $999, the award-winning system featured the following configuration:
- Intel® Core® i5-3470 processor
- Intel® BOXDH77EB mATX motherboard
- 8GB of DDR3-1600 Patriot® Memory
- NVIDIA® GeForce® GTX 650 Graphics
- 1TB 7200 RPM Hard Drive
- 32GB Intel 525 Series mSATA drive for Intel Rapid Start and Smart Response Technology
- Windows® 8 (Windows 7 also available)
All Velocity Micro desktops are hand built and supported in the USA. To custom configure the award winning Vector™ Z25 or any other home office, workstation, enthusiast, or gaming desktop, notebook, or All-in-one, visit VelocityMicro.com.
About Velocity Micro, Inc.
Velocity Micro, Inc. is the premier high-performance personal computer provider in North America. Founded in 1992, Richmond, Va.-based Velocity Micro custom builds award winning gaming, mobile, multimedia, small business, workstation, visual supercomputers and peripherals. Velocity Micro products are currently available at most consumer electronics retailers nationwide. For more information, please call (800) 303-7866 or visit www.VelocityMicro.com
Velocity Micro has earned over 60 industry awards, including 17 PC Magazine Editor's Choice awards. CNET, Maximum PC, Mobile PC, PC World, Computer Gaming World, Computer Shopper, and PC Gamer editors have all chosen Velocity Micro systems as some of the industry's best-performing, highest-quality, and most reliable PCs.
Media Contact:
Josh Covington
804.897.6166 x211


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The charge that part of Iraq's weapons of mass destruction programme went to Syria before the war came from the ex-head of the US weapons inspection team.
David Kay said former Iraqi officials had given evidence that materials had been moved across the border.
However Syrian Information Minister Ahmad al-Hassan said the allegations were "baseless deception and lies".
The minister told reporters in Damascus that the US was seeking to cover up its failure to find any weapons stockpiles in Iraq, despite using them as a pretext for last year's war.
The accusations by Mr Kay came in an interview published in Britain's Sunday Telegraph newspaper.
"We are not talking about a large stockpile of weapons," he told the paper.
"But we know from some of the interrogations of former Iraqi
officials that a lot of material went to Syria before the war,
including some components of Saddam's WMD programme.
"Precisely what went to Syria, and what has happened to it, is a major issue that needs to be resolved."
Mr Kay resigned on Friday as leader of the Iraq Survey Group and has been replaced by Charles Duelfer.
Ten months after the US-led war in Iraq that toppled Saddam Hussein, the team in charge of the search for banned weapons has yet to find them.
On Saturday, US Secretary of State Colin Powell cast doubt on the existence of weapons stockpiles in Iraq, saying it was an "open question" whether they existed.


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- Research
- Open Access
Functional identification in Lactobacillus reuteri of a PocR-like transcription factor regulating glycerol utilization and vitamin B12 synthesis
- Filipe Santos†1, 2, 6,
- Jennifer K Spinler†3, 4,
- Delphine MA Saulnier†3, 4, 7,
- Douwe Molenaar1, 2,
- Bas Teusink1, 2,
- Willem M de Vos5,
- James Versalovic3, 4 and
- Jeroen Hugenholtz2, 6Email author
© Santos et al; licensee BioMed Central Ltd. 2011
- Received: 3 February 2011
- Accepted: 21 July 2011
- Published: 21 July 2011
Lactobacillus reuteri harbors the genes responsible for glycerol utilization and vitamin B12 synthesis within a genetic island phylogenetically related to gamma-Proteobacteria. Within this island, resides a gene (lreu_1750) that based on its genomic context has been suggested to encode the regulatory protein PocR and presumably control the expression of the neighboring loci. However, this functional assignment is not fully supported by sequence homology, and hitherto, completely lacks experimental confirmation.
In this contribution, we have overexpressed and inactivated the gene encoding the putative PocR in L. reuteri. The comparison of these strains provided metabolic and transcriptional evidence that this regulatory protein controls the expression of the operons encoding glycerol utilization and vitamin B12 synthesis.
We provide clear experimental evidence for assigning Lreu_1750 as PocR in Lactobacillus reuteri. Our genome-wide transcriptional analysis further identifies the loci contained in the PocR regulon. The findings reported here could be used to improve the production-yield of vitamin B12, 1,3-propanediol and reuterin, all industrially relevant compounds.
- Empty Plasmid
- Functional Assignment
- Chemically Define Medium
- Lactobacillus Reuteri
- Glycerol Utilization
Lactobacillus reuteri is a heterofermentative lactic acid bacterium colonizing the gastrointestinal tract (GI tract) of various mammals, including humans [1]. It is able to convert glycerol to 1,3-propanediol in a two-step enzymatic conversion, yielding NAD+[2]. In the first reaction, glycerol dehydratase (EC 184.108.40.206), converts glycerol to 3-hydroxypropionaldehyde requiring the presence of vitamin B12 as a coenzyme [3]. Reuterin, a mixture of 3-hydroxypropionaldehyde isomers [4], is a potent antimicrobial, bestowing L. reuteri with an important growth advantage over other residents of the GI tract, such as Gram-negative enteric bacteria [5, 6].
We have shown previously that L. reuteri CRL1098 encodes the complete machinery necessary for de novo synthesis of vitamin B12 in a single chromosomal gene cluster [7]. This cluster was shown to be very similar to that present in various representatives of γ-Proteobacteria, standing out against canonical phylogeny. Complete genome sequence analysis of the type strain of L. reuteri revealed that the region immediately upstream of the vitamin B12 biosynthesis cluster maintains a gene order similar to that of Salmonella[8]. The functionality of this upstream region was demonstrated to also match Salmonella where the pdu gene cluster is located. The latter encodes the assembly machinery of metabolosomes and the several subunits of a large diol dehydratase that can metabolize both glycerol and 1,2-propanediol [9].
Also within this cluster resides a gene (lreu_1750) predicted to encode a 359 amino acid long putative transcription factor of the AraC type family, containing a typical helix-turn-helix domain. Based strictly on its conserved genomic context, this gene has been suggested to encode PocR, a regulatory protein that modulates propanediol utilization (pdu) and vitamin B12 biosynthesis in enteric bacteria [8–10]. This functional annotation, however, does not seem to be fully supported by sequence homology. And more importantly, to the best of our knowledge, it completely lacks experimental confirmation.
Here we provide the first experimental evidence to support the functional assignment of Lreu_1750. This was achieved by overexpression and inactivation of lreu_1750, assessing its impact on central carbon and energy metabolism, and on reuterin and vitamin B12 synthesis. In addition, we characterized the genome-wide transcriptional response of both constructs in comparison to their parent strains leading to the identification of the genes comprised in the PocR regulon of Lactobacillus reuteri.
Results and Discussion
Phylogenetic analysis of Lreu_1750
Physiological effects
The physiological effects observed for the overexpression and inactivation of Lreu_1750 are all in agreement with its functional assignment as the regulatory protein PocR.
Transcriptomic response
In order to probe the global regulatory role of the putative PocR of L. reuteri, we compared the transcriptomes of the deficient and overexpressing strains relative to their parent strains. Considering that (i) glycerol has been shown to induce the expression of lreu_1750[10], masking the effect of its overexpression; (ii) consequently the differentiating phenotype of the PocR deficient strain can be best observed under conditions in which its growth kinetics are hampered (such as in the presence of glycerol - Figure 2); and (iii) there is a large redundancy between the different transcriptome analyses carried out; most emphasis in this report has been put on the data related to the lreu_1750 overexpression in the absence of glycerol. The complete list of differentially regulated genes under all conditions assayed is available in Additional file 1: Transcriptome analysis data.
Relative expression levels of loci associated to PocR and not within its flanking region a.
M b.
p c.
Accession number
Transcriptional regulator, LacI family
3-hydroxybutyryl-CoA dehydrogenase (EC 126.96.36.199)
Putative cobalt-transporting ATPase d.
Putative cobalt-transporting ATPase d.
Arabinose-proton symporter
Pyruvate dehydrogenase alpha subunit (EC 22.214.171.124)
Pyruvate dehydrogenase beta subunit (EC 188.8.131.52)
Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 126.96.36.199)
Alpha-galactosidase (EC 184.108.40.206)
Transcription regulator, Crp family
Fumarate hydratase (EC 126.96.36.199)
Lactose permease
Histidine decarboxylase (EC 22.214.171.124)
The transcriptome studies carried out for the PocR insertion mutant were consistent with the results obtained through the overexpression of lreu_1750. We mainly observed in the PocR mutant compared to the wild-type strain, a down-regulation of the genes located within the genetic island that comprises the pdu and vitamin B12 operons (Additional file 1, Table S3). Again due to the rarity and fragility of these transcripts [7, 14] only 30 out of 58 loci are differentially expressed significantly (p-value ≤ 0.05) even though the whole region, excluding the transposase, appears co-regulated.
There is strong phylogenetic evidence supporting that the pdu and vitamin B12 synthesis gene clusters have been acquired by L. reuteri through distant horizontal gene transfer [7, 8]. The confinement of the PocR regulon to mostly one continuous stretch of the chromosome (Figure 6), with exception of the putative cobalt transporter, further substantiates this hypothesis.
In this study, we have provided experimental evidence that lreu_1750 encodes a PocR-like regulatory protein, despite its lack of sequence homology to PocR from enteric bacteria. This was achieved by overexpression and inactivation of lreu_1750, and assessment of its impact on central carbon and energy metabolism, and on reuterin, 1,3-propanediol and vitamin B12 biosynthesis. In addition, we characterized the genome-wide transcriptional response of both constructs in comparison to the wild-type leading to the identification of the genes encompassed in the PocR-like regulon of L. reuteri. The latter were found to be similar to the ones present in some representatives of γ-Proteobacteria. Ultimately, the demonstrated stimulatory effects of PocR on vitamin B12, 1,3-propanediol and reuterin synthesis could be applied to improving the production yield of these industrially relevant compounds.
Phylogenetic analysis of Lreu_1750
The sequence of Lreu_1750 (GI:148544956) was entered as a string to search for closely related homologs within available microbial genomes using the protein-protein BLAST algorithm [19]. Relevant sequences were retrieved and aligned using ClustalW with default settings [20] and visualized in CLC Sequence Viewer 6.5.
Strains, plasmids, primers and cultivation conditions
Strains, plasmids and primers used in this study
Relevant features
Source or reference
L. reuteri
Type strain, synonymous to ATCC 23272, DSM 20016 and F275. Human isolate.
Japanese Collection of Microorganisms (Riken, Japan)
L. reuteri
Synonymous to MM4-1A. Finnish mother's milk isolate.
Biogaia AB (Stockholm, Sweden)
EmR, pocR insertion mutant derivative of L. reuteri ATCC PTA 6475
This study
Lc. lactis
MG1363 pepN:nisRK, cloning host.
NIZO culture collection (Ede, The Netherlands)
L. delbrueckii
L. delbrueckii subsp. lactis ATCC 7830. Vitamin B12 assay indicator strain.
NIZO culture collection (Ede, The Netherlands)
Used in routine cloning and to construct pJKS100
Invitrogen (Carlsbad, CA)
L. reuteri replication origin used to construct pJKS100
CmR, pNZ8148 derivative with the nisin promoter replaced by the pepN promoter
CmR, pNZ7021 derivative harboring lreu_1750 downstream of the pepN promoter.
This study
CmR, repA-positive temperature-sensitive derivative of pWV01
EmR, repA-negative derivative of pWV01
EmR, pORI28 derivative harboring internal fragment of gene encoding putative PocR
This study
CmR, E. coli-L. reuteri shuttle vector
This study
CmR, pJKS100 derivative expressing 6475 pocR gene under control of its natural promoter
This study
5' - 3'
Amplification of lreu_1750 and addition of Kpn I site
Amplification of lreu_1750
Control of pNZ7748
Amplification of internal fragment of putative pocR, addition of Bam HI site and translational stop codon
Amplification of internal fragment of putative pocR and addition of Eco RI site
Amplification of wild-type pocR gene and natural promoter
Amplification of wild-type pocR gene and natural promoter
Construction of putative pocR overexpression and deletion mutants
Gene lreu_1750, encoding the putative PocR in JCM1112T, was overexpressed constitutively under control of the pepN promoter in a similar fashion as previously described [22]. A fragment containing lreu_1750 was amplified from chromosomal DNA of L. reuteri using Herculase II DNA polymerase (Stratagene, La Jolla, USA), and primers P180 and P181 (Table 2). After digestion with Kpn I, the modified amplicon was purified and cloned in pNZ7021 making use of the Kpn I and Pml I restriction sites directly downstream of the pepN promoter. The resulting plasmid, termed pNZ7748, was used directly from the ligation reactions to transform Lactococcus lactis NZ9000 by electroporation [23]. Subsequently, pNZ7748 was purified from Lc. lactis as previously described [24] and, after confirming the sequence of the insert using both P181 and P182, it was used to transform L. reuteri also by electroporation [25].
The disruption of the putative pocR gene was carried out in L. reuteri ATCC PTA 6475, which shares an identical sequence with the type strain (JCM1112) for this region of the chromosome [1]. This was achieved by site-specific integration of plasmid pORIpocR as described previously [26] using the temperature-sensitive plasmid pVE6007 [27] as the helper plasmid. The internal fragment of the target gene was amplified by PCR using primers LR0062F-BHI and LR0062R-ERI (Table 2), and inserted into pORI28 by directional cloning using standard techniques [24]. The resulting insertion mutant was designated 6475::pocR.
Complementation of L. reuteri 6475::pocR
An E. coli-L. reuteri shuttle vector (pJKS100) was constructed by combining an L. reuteri replicon from pLEM5 [28], the chloramphenicol resistance gene (CmR) from pVE6007 [27], the L. lactis promoter (P23) [29], and the pUC origin and multiple cloning site (MCS) from pCR®2.1 (Invitrogen, Carlsbad, CA). Each fragment was PCR amplified from their respective template, restriction enzyme digested and subsequently ligated to generate the final shuttle-vector, pJKS100. To create the complementation vector for 6475::pocR, the L. reuteri 6475 pocR gene with its natural promoter was PCR-amplified from genomic DNA using LR0062 FL F and LR0062 FL R primers and cloned into pJKS100 using standard techniques [24]. Both constructs, pJKS100 and pJKS101, were electroporated seperately into L. reuteri 6475::pocR as previously described [25].
Fermentation conditions and substrate and product analysis
The physiological effects of the overexpression of lreu_1750 were studied in pH-controlled batch cultivations of L. reuteri pNZ7748 (lreu_1750 overexpression) and L. reuteri pNZ7021 (empty plasmid) in CDM in the presence or absence of glycerol carried out as described previously [14]. At different time points, samples were taken for transcriptome, supernatant and vitamin B12 analysis (Figure 2). We determined the extracellular concentration of main fermentation substrates and products by HPLC, as described elsewhere [6, 30].
The comparison between the insertion mutant, 6475::pocR, and its parent strain was established in batch fermentations of LDMIIIG or MRS carried out in an anaerobic chamber (80% N2, 10% H2, and 10% CO2; Microbiology International). Transcriptome comparisons were carried out at the end of the fermentation when biomass concentration became stable.
Vitamin B12 and reuterin determination
Vitamin B12 levels were determined as described in the Official methods of analysis of AOAC International [31], using a bioassay with L. delbrueckii subsp. lactis ATCC 7830 as the indicator strain. Reuterin production was measured with a bioassay and carried out as previously described [5].
Transcriptome analyses
- Transcriptional analysis of the putative PocR overexpression mutant
The transcriptome of cells transformed with pNZ7748 (lreu_1750 overexpression) and pNZ7021 (empty plasmid) were compared using cDNA microarrays as previously detailed [14] using a hybridization scheme comprising 17 arrays in a loop-design. The following samples were hybridized per array labeled with cyanine3 and cyanine5, respectively: sta-F6 and sta-F5, sta-F7 and sta-F8, sta-F5 and sta-F7, sta-F8 and sta-F6, sta-F3 and sta-F4, sta-F1 and sta-F3, sta-F2 and sta-F1, sta-F4 and sta-F2, exp-F3 and exp-F4, exp-F1 and exp-F3, exp-F2 and exp-F1, exp-F4 and exp-F2, exp-F4 and sta-F4, sta-F3 and exp-F3, sta-F2 and sta-F8, sta-F4 and sta-F6, exp-F2 and sta-F2. Here, F1 and F5 represent completely independent biological duplicates of L. reuteri pNZ7021 cultured in the absence of glycerol; F2 and F6 represent completely independent biological duplicates of L. reuteri pNZ7748 cultured in the absence of glycerol; F3 and F7 represent completely independent biological duplicates of L. reuteri pNZ7021 cultured in the presence of glycerol; and F4 and F8 represent completely independent biological duplicates of L. reuteri pNZ7748 cultured in the presence of glycerol. The prefix exp- and sta- stand for cells harvested at mid-logarithmic and early-stationary growth phases, respectively. The custom probe design of the Agilent 11 K microarray platform (Agilent Technologies, Santa Clara, CA, USA) used is available at the Gene Expression Omnibus http://www.ncbi.nlm.nih.gov/geo under accession number GPL6856, and the data obtained were deposited in the same repository under accession number GSE13289.
- Transcriptional analysis of the putative PocR insertion mutant
The transcriptome of the insertion mutant, 6475::pocR, and its parent strain were compared using two-color microarrays as previously detailed [32]. Briefly, oligonucleotides (60-mers) were designed and synthesized for 1,966 open reading frames from a draft genome sequence of L. reuteri ATCC PTA 6475 [1]. For expression analyses, three biological replicates of the insertion mutant and parent strain were compared. Moreover, dye-swap hybridization was performed for each comparison. Following mRNA isolation [32], cDNA synthesis, labeling, and hybridization were performed as previously described [32]. Information regarding the microarray platform and data obtained is deposited at NCBI Gene Expression Omnibus (GEO; http://www.ncbi.nlm.nih.gov/geo) under GPL7541 and GSE22926, respectively.
GenePix Pro 4.0.12 software was utilized for image analysis of the 6475 microarrays. Normalization within arrays and between arrays was performed by applying the Loess algorithm [33] using the Limma package [34] in R http://www.r-project.org. Normalized intensities were used for further analysis. The average signal intensities of three biological replicates were calculated in order to compare the relative gene expression of mutant and wild type strains. The statistical significance of differences was calculated based on variation in biological duplicates, using the eBayes function in Limma (cross-probe variance estimation) and false discovery rate (FDR) adjustment of the p-values. Only genes that were differentially expressed by least 1.5-fold with FDR-adjusted p-values lower than 0.05 were considered significant.
This work was supported by the Department of Defense through the Defense Advanced Research Projects Agency (DARPA), National Institutes of Health, National Institute of Diabetes, Digestive and Kidney Diseases (R01 DK065075), and National Center for Complementary and Alternative Medecine (R01 AT004326). We thank Eammon Connoly for providing the L. reuteri 6475 strain, P. Hemarajata and M. Balderas for their technical efforts, and TA Misttetta for assistance in the statistical analyses of the 6475 microarray data.
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Saturday, September 16, 2017
Warrior Princess
This is my best friend, Evryn, who was gracious enough to model for me a bit during my visit a few weeks ago, as well as be the photographer for my last two Sinful Sunday entries. She is also the model for the header image featured in Star Talker: Part 12: Conditions. She had recently dyed her hair white, and I wanted to take advantage of her elfin aesthetic. It also helps that she and her husband have some interesting weapons such as the sabotage spear pictured above and a kickass black gladius, both labeled for "home defense." I really like the way this turned out.
13 comments:
Evryn is gorgeous. I love this pose, her breasts and the way she is holding the weapon, almost in admiration, love, respect, or is she just thinking 'I can really do some damage with this fucker'? So sexy, eyes half closed and mouth part open, almost as if she is having an orgasm while stroking the shaft of the spear.ReplyDelete
I thought your art work on #12 was outstanding and fun to see your model Her breast with the chains is exquisite and did turn me on as does this image. I think one of your best.
This is such an awesome shot, I love the way her hair is flowing out and falling across her chest. Warrior Princess is the perfect title for this shotReplyDelete
Wow - Such an awesome edit <3 Really highlights all the little details xReplyDelete
Great picture and edits. Its always nice to have a willing and wonderful model to work with.ReplyDelete
I agree with Molly, the hair relay adds to the image.ReplyDelete
This is beautiful, love it xxReplyDelete
It really is a gorgeous image!ReplyDelete
Rebel xox
I always adore your unique photographs! This is a fantastic image and such a beautiful model!ReplyDelete
Aurora x
Love the power in this image!!!ReplyDelete
Xxx - K
This is Evryn, first of all thank you so much for the feature, Simina! This was incredibly fun to do. The spear in the photo is actually a ritual tool of mine that demands respect. I'd love to do this again in the future. Thank you all for your wonderful comments, I'm flattered!ReplyDelete
Excellently posed and takenReplyDelete
What a great edit, I love how it picks out the details.ReplyDelete
Great picture!ReplyDelete
Unearthly colours! Dramatic and sexy!


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It's a lonely place to be a Bengals fan, but some in Reliant Stadium couldn't help but show their colors.
Matthew Brewer, 24, and his father Bryan Brewer, 53, wore Bengals jerseys for quarterback Andy Dalton in a crowd of Texans fans. Dalton is from Katy.
"That's why I bought them, so I wouldn't get yelled at," said Matthew Brewer, of Cincinnati.
Bryan Brewer, of Cypress, said he was conflicted even though he wore a Dalton jersey to the game. He had a Texans and Bengals towel with him.
"That's how conflicted I am," he said.
Billy Smith, 49, was sitting behind the pair in Bengals jerseys, the two lone Cincinnati fans in a sea of blue and
"He's schizophrenic," Smith said, as other nearby fans began heckling the fans before kickoff.
Photo: Johnny Hanson / Houston Chronicle


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In my 30 plus years in the commercial building industry, as a superintendent, I have overseen construction projects from $500 thousand to $10 million with extreme satisfaction from the customer and employer. Throughout my years in the trade, I built both of my family homes and several presale homes. The next logical step and challenge in my career was to form our own construction company. Mink Construction, LLC was established in 2004. We value our reputation and take great pride in what we do. This is evident in every project we do from start to finish.
~ Paul Minkler & Family