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What are some things we can receive from God? What can we expect and should we expect to receive from God? Is life in
nothing more than salvation or just as we exchanged our life for his can we
expect to receive of his fullness? Have we truly become children in his
kingdom? Is he not our father now and us his children? I'm going to cover just some of the WHATS
that we can expect or that we have already received!
New King James Version (NKJV)
12 But as many as received Him, to them He gave the right to become children of God, to those who believe in His name: 13 who were born, not of blood, nor of the will of the flesh, nor of the will of man, but of God.....
16 And[a] of His fullness we have all received, and grace for grace.17 For the law was given through Moses, but grace and truth came through Jesus Christ.
We have already received him and gained the right to be called "the children of God." Many people say that all of mankind are God's children but the bible teaches that receiving sonship comes through receiving his son,
Christ. Only those who have received him, according to this scripture
have the right to be his children. Jesus himself said, "You must be born again," and
here John says we are those who have
been born not by flesh and blood but by the will of God.
When you are a son, living in the house, the fullness of what the father has is yours so you can expect to receive from the hand of the father all that you need and all that he has. All of his kingdom is available and accessible to you! You need to worry about your needs. You need only expect to receive.
31 "Therefore do not worry, saying, 'What shall we eat?' or 'What shall we drink?' or 'What shall we wear?' 32 For after all these things the Gentiles seek. For your heavenly Father knows that you need all these things. 33 But seek first the kingdom of God and His righteousness, and all these things shall be added to you. 34 Therefore do not worry about tomorrow, for tomorrow will worry about its own things. Sufficient for the day is its own trouble.
We need not worry about receiving from God. The key to receiving is seeking God. As we seek him and his ways, he will provide for all of our needs. He knows what we need. As we seek him and his ways, "all these things" will be added. What things? He's speaking of needs- the need to eat, drink have clothes and other necessities. God will take care of us and we need to be in a state of expecting to receive of his blessings and in a state of casting all of our cares upon him! In this passage we are told to be concerned with each day. As we walk in that state of expectancy we'll see each days needs met.
I posted in an earlier blog concerning sowing and reaping. This is a principle found in the bible. God wants to move through us and provide through us. He wants to establish his Kingdom through our hands. We are vessels and if he knows he can get it through us, he will get it to us. This isn't meant to be a thorough teaching on sowing and reaping or on finances but you can go here to read more:
This is an area of contention in the body of
that shouldn't be. While it is true that the love of money is the root of all
evil, money is not. Almost all churches
believe in offerings and understanding God's teaching about them is something
every child of God should understand. The principle of sowing and reaping
endures forever. I'll just list a couple of passages showing the truth that God
desires to prosper his people!:
2 Beloved, I wish above all things that
thou mayest prosper and be in health, even as thy soul prospereth.
Psalm 35: 27 Let them shout for joy, and be glad, that favour my righteous cause: yea, let them say continually, Let the Lord be magnified, which hath pleasure in the prosperity of his servant.
Looking for something? Don't stop until you find it. The bible teaches that whatever we seek we will find. We have to press in to God. So many ask for an answer to prayer and give up when the answer doesn't come right away. We need to believe what the Word of God says and although we don't have to beg, we also need to continually seek God and let him know in prayer we are believing and continually knocking until we see the answer. Delay is not denial. Sometimes its just a matter of timing. God hears the moment you ask and you also receive the moment you believe. This is where standing in faith comes in. Believe that you have received the answer from God and then wait patiently for the arrival, even unto death. The bible says
stayed in faith believing the things he heard from God's Word. Some of what God
promised him is even today coming to pass!
MATTHEW 21:22 And whatever things you ask in prayer, believing, you will receive."
One sure way to receive from God is to not give up until you receive. Don't ever back down. Don't ever stop believing!
One of the things we receive of his fullness is the promise of eternal life. All Christians believe they will live in heaven forever. The same way we receive eternal life, we receive the other blessings that flow from his fullness so why do we find it so hard to receive?
MATTHEW 19:29 And everyone who has left houses or brothers or sisters or father or mother or wife or children or lands, for My name's sake, shall receive a hundredfold, and inherit eternal life.
When we gave up our life in exchange for his, we did receive the promise to inherit eternal life. What a great exchange! A promise that this life on earth is not all there is but we live a life that goes on forever.
I'm thankful that not all Christians have to turn their backs on family members. Here, in
America we don't realize the
persecution many in other nations go through just to be able to believe in Christ. Some are disowned by their families or threatened
with death just to become believers in Christ. This
scripture promises any person that gives up anything to become Christ's
can expect to receive from God in this life and eternally! This is not only an American gospel. This
gospel is for the world and while we are persecuted in America, some
of us could not imagine what it's like in other nations when you become a
follower of Christ!
13 And He said to them, "Do you not understand this parable? How then will you understand all the parables? 14 The sower sows the word.15 And these are the ones by the wayside where the word is sown. When they hear,
Satan comes
immediately and takes away the word that was sown in their
hearts. 16 These likewise are the ones sown on stony ground who, when
they hear the word, immediately receive it with gladness; 17 and they
have no root in themselves, and so endure only for a time. Afterward, when
tribulation or persecution arises for the word's sake, immediately they
stumble. 18 Now these are the ones sown among thorns; they
are the ones who hear the word, 19 and the cares of this world,
the deceitfulness of riches, and the desires for other things entering in choke
the word, and it becomes unfruitful.20 But these are the ones sown on good
ground, those who hear the word, accept it, and bear fruit: some
thirtyfold, some sixty, and some a hundred."
Some receive the Word by the wayside- they hear, but
Satan takes it away immediately.
Some receive the Word on stony ground- they receive gladly but have no root or endurance through tribulation.
Some receive the Word on thorny ground- they are consumed with cares & the DECEITFULNESS of riches and desire for OTHER THINGS. They pay more attention to the world than the Word and are unable to receive.
Some receive the Word on GOOD GROUND- they hear, accept, bear fruit- 30, 60, 100 fold.
HOW TO RECEIVE INTO GOOD GROUND? Be constant. Receive gladly
AND endure. Don't be distracted.
Grow roots into the kingdom.
This may be the most important thing we receive from God. When we receive the Word into good, attentive, soft and yielding soil in our heart, we can expect to receive the fruit that Word brings. When we tend to our heart as a good farmer does his soil and we place God above all else, and yield to the teachings of his Word, there will be lasting fruit in our lives. You may say, "What fruit?" The fruit produced by believing all things found in the Word of God. The Word of God is a life changer. The Word of God is alive and able to bring needed change to the person who is willing to listen and learn.
HEBREWS 4:12,13 For the word of God is living and powerful, and sharper than any two-edged sword, piercing even to the division of soul and spirit, and of joints and marrow, and is a discerner of the thoughts and intents of the heart.13 And there is no creature hidden from His sight, but all things are naked and open to the eyes of Him to whom we must give account.
Let's commit to allowing the Word to do a living and powerful work in our heart, changing us into vessels for his glory!
Christ, there is healing,
finances, answers to prayer, and many blessings too numerous to mention. Let's follow the most important teaching and
that is to allow his Word to work. Seek it today, find out what it says and
allow it to be planted deeply into the soil of your heart. The blessings the Word will bring are too
numerous to mention! Amen!


text:
2007 – Qatar Airways CEO Akbar al-Baker warns Airbus that he will order Boeing 787 Dreamliners if a better design for the A350 is not forthcoming. A month later, al-Baker drops the threat after Airbus cedes to his demands and eventually orders 80 of the new jets.
2002 – The crew of Ryanair Flight 296, a Boeing 737-800 (EI-CSA), while taxiing to a gate at London Stansted Airport evacuates the plane after fire personnel report smoke and fire billowing from the right engine. All 117 passengers are evacuated in 90 seconds, but flight attendants have difficulty opening the doors without assistance. Without proper guidance, several passengers evacuate over the right wing not knowing that's where the fire is suspected. An investigation reveals there had been no fire, and UK aviation safety regulators recommend several changes to evacuation procedures and training at Ryanair.
1993 – The US Air Force begins dropping supplies over wartorn Bosnia.
1910 – Pioneering Lockheed Skunk Works aerospace engineer Kelly Johnson is born in Ishpeming, Michigan. He would spend four decades at the company, launching Area 51 and developing many of history's most advanced aircraft, including the U-2 and SR-71 Blackbird. In a 2003 Aviation Week list of the top 100 "most important, most interesting, and most influential people" of aviation's first century, Johnson was ranked 8th. He passed away at the age of 80 in 1990.

subset: commoncrawl


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Dear All,
The pace of events in Iran has slowed down. The mood is not jubilant. But the "green movement" is not defeated either. An eye witness talking to people picnicking in a park in Tehran, described the calm as a layer of ash covering burning charcoals. Last week, before the major crackdown, Tehran witnessed a massive silent rally. The majority of Iranians grow up with poetry. They are familiar with the poetic power of silence. Many of the banners carried messages that showed this awareness. I picked one image from a silent marcher who carried a sign with a beautiful line of poetry (see image and translation to the right).
As the government works to push the struggle for freedom from the streets to jails, a new (but powerful) kind of silence needs to be recognized and given voice. Many poets, musicians and artists have already started to do that.
"Iran, the Land of Hope" Maestro Shajarian Sings a New Song
* Here is a song gifted to the new Iran emerging from the 2009 election by the one of the greatest musicians of present day Iran, Mohamad Reza Shajarian. And he rightly calls it "Iran, the land of hope." Enjoy:
Makhmalbaf's New Message to Mr. Mousavi
* Mr. Mousavi's Facebook has posted a new video today with a message addressed to Mr. Mousavi delivered by the renowned Iranian film maker, Mohsen Makhmalbaf, on behalf of Iranians who have contacted the latter. The main thrust of this message is that Mr. Mousavi should not wait for obtaining a permit from the government to revive public gatherings and rallies.
Internal Economic Strategies of Isolating the Government
* Letters are circulating in Iran with recommendations for economic measures to weaken the grip of Mr. Ahmadinejad's government on power. The recommendations include
1. Refusing to give ads to the state run TV, and newspapers serving as the voice of the government.
2. Pulling out money invested in banks and financial institutions run by the government or by the revolutionary guards.
3. Refraining from contributing to the charity boxes scattered throughout towns all across the country.
Short Clip of a Pick up Truck Driving into Crowds on June 23
* Mr. Mousavi's Facebook has posted a very short video clip showing a pick up truck driving into a large crowd of peaceful demonstrators:
Two More Grand Ayatollahs Speak up Against the Regime
* So far, at least three Grand Ayatollahs (all with substantial followings) had spoken against the behavior of the government towards those objecting to the conduct of the election: Ayatollahs Montazeri, Sanei, and Safi Golpaigani. Two more strong voices have now been added:
a. Grand Ayatollah Bayat-Zanjani has declared that, according to the constitution, people have a right to public assembly. The burden is on the government to address peoples' legitimate demands. If the government does not do so, it will have committed malfeasance (khalaaf) and is responsible. (Quick and abbreviated translation from Farsi). Full article here: http://bayatzanjani.net/fa/faq/question-112.html.
b. Ayatollah Mousavi-Ardebili has said that "we should not silence people by using brute force." Link here: http://news.gooya.com/politics/archives/2009/06/089997.php
Irandokht Celebrates Iranian Women's Participation in the Move toward Social Change
* Before closing this window, visit the latest Irandokht news site: http://www.irandokht.com/. It celebrates the lively participation of Iranian women in the leap toward social change.
Have a great Sunday. Good Night.
Fatemeh Keshavarz, Professor and Chair
Dept. of Asian and Near Eastern Languages and Literatures
Washington University in St. Louis
Honorary Co-Chair, Iranians For Peace
Tel: (314) 935-5156
Fax: (314) 935-4399


text:
December 3, 2015 - 12:00 pm
December 3, 2015 - 1:00 pm
AddressView map
Rare Earth Nitrides: The New Kids On The Block For Semiconductor Spintronics
The rare earth nitrides (RENs) are almost unique materials, displaying the properties of both semiconductors and magnets, even in their intrinsic state. They offer opportunities across a broad range of electronic-optical technologies and "spintronics" applications, an emerging energy-saving branch of electronics which utilises both the charge and the spin of electrons. Non-volatile random access memory, reduced energy consumption, increased data processing and storage speed are only three examples of a very long list. To date the research on the RENs has focussed on the fundamental properties, both from experimental and theoretical point of views. One particular aspect in this field that has been largely overlooked so far is the question of the epitaxial growth. Here, I will take stock of where our progress has occurred within recent years in both the epitaxial growth and experimental field in view of developing proof-of concept spintronic device based on RENs. Maybe even more interesting is the RENs are epitaxy-compatible materials with group III-nitrides, the technologically important nonmagnetic semiconductor family for the fabrication of white and blue light emitting diodes and transistors. I will present some of the results.
Victoria University of Wellington, RB901
University of Canterbury, Psychology 164


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Ralph Schrader began his career in 1980 as a lighting designer in school. He later founded RS Rockservice GmbH & Co. KG with offices in Hong Kong and Shanghai. Ralph's career started with rigging and lighting with bands such as Pink Floyd, Paul McCartney, and David Bowie. Later he transitioned into exhibitions in Europe with Tchibo, John Deere, HP, Caterpillar, Schwarzkopf, Siemens.
In the early 1990s Ralph began working globally with major automotive brands such as BMW and VW. He engineered the rigging and did lighting design in cities such as Moscow, San Paulo, Frankfurt, Geneva, Beijing, Shanghai, Tokyo, Los Angeles, and Detroit. Ralph's strengths are design, engineering, and to find solutions for his clients.


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Florida State Seminoles head coach Jimbo Fisher has hit out at college football legends Bo Jackson and Herschel Walker after they criticized his quarterback Jameis Winston following his latest run in with the authorities, this time for potentially being paid to sign memorabilia.
Heisman Winners Hating
On Tuesday, former Auburn star Bo Jackson -- the 1985 Heisman winner -- said in an interview on the Jim Rome Show that Winston "needs to make some changes in his life" following a string of off-field incidents including an ongoing school investigation for a 2012 sexual assault allegation. Jackson, who like Winston is from Bessemer, said he was "washing his hands" of Winston after the 2013 Heisman Trophy winner ignored his advice.
Related Articles:
- Gurley to miss more games
- Bo Jackson gives up on Winston
- Hundley to sign with Jay Z
On Thursday, Herschel Walker -- who won the Heisman at Georgia in 1982 -- told the SEC Network's Paul Finebaum that Winston did not get his Heisman vote last year and wasn't likely to this year, either. (Former Heisman winners are included in the award's annual voting pool for life).
Fisher's Retort
Fisher was a guest on Mike & Mike in the Morning on ESPN Radio Friday, and was asked for his thoughts on both Jackson and Walker's comments. He dismissed both sarcastically.
"Bo [lived] in Bessemer with Jameis for 18 years and never spoke to him, so why would he speak to him now?," said Fisher, a former Auburn offensive coordinator. "He never knew Bo. Bo had lived in the same neighborhood. Bo can wash his hands. That's fine.
"... For Herschel ... that's wonderful. What Herschel and Bo think ... we're moving on. That has no effect on us."
Honest Winston? Ha!
The best bit of the interview however, was undoubtedly when Fisher said that Winston had "always been truthful and honest with me." Yep that's the same Jameis Winston that was suspended for a game mere weeks ago by FSU officials for lying about an incident. Genius.
Winston has garnered quite the impressive rap sheet in his three years at Florida State. On top of the alleged sexual assault that was bungled by the authorities, Winston has been handcuffed at gunpoint for carrying a pellet gun on campus and accused of breaking windows in an apartment complex later that same day, accused of stealing soda from a restaurant fountain and cited for shoplifting crab legs from a grocery store.


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Bluff City Pendant, Small
Design Within Reach
Born in Tokyo and raised in New England, Jonah Takagi earned his bachelors degree from the Rhode Island School of Design in 2002. Pursuing his two passions, he has spent the intervening years designing furniture and playing bass guitar for several indie rock bands. Unfortunately, being on the road gave him little time to showcase his furniture, leaving him with a house full of products no one had seen. To showcase his growing body of work, he founded Atelier Takagi in 2005. With a nod toward the Memphis movement, Takagi borrowed that American citys nickname for his Bluff City Pendant (2009). A mix of industrial and refined aesthetics, this pendant features a bulb enclosed in a metal cage on the top for ambient lighting and a solid metal shade on the bottom for concentrated downward lighting. Bluff City was nominated for a Dwell magazine Modern World Award in 2011. Bulb (not included): G40 incandescent 60W/120V/E26 medium base. UL Listed. Made in China.
Product Specifications
- Lead Time
- 0-3 Weeks (Quick Ship)


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EPSRC Centre for Doctoral Training in Metamaterials
View All CDTs
The University of Exeter invites applications for 4-year fully-funded PhD studentships starting in September 2018 as part of its EPSRC Centre for Doctoral Training in Metamaterials. This new centre-of-excellence in metamaterials, also incorporating topics from graphene science, magnetics, transformation optics, nanocomposites and quantum systems, acts as a hub of international and industrial collaborations, training scientists and engineers with the relevant skills, knowledge and professional attributes to transform the exploitation of material science across industry and academia.
We invite applications from independently-minded undergraduates that have demonstrated excellence at the undergraduate level in a relevant subject. Applicants should have an interest in electromagnetics, acoustics, nanotechnology and/or materials science, and are expected to demonstrate creativity and be open-minded to collaborative work and innovation.
Our Centre consists currently of over 60 postgraduate researchers from around the UK, Europe and the globe. Our UK and EU students receive a stipend to cover living expenses and fees, and we are normally able to offer small number also to International students. Industry-orientated studentships may also include a top-up to the stipend.
Further information can be obtained by e-mail to [email protected] or by visiting our website.
Metamaterials have properties beyond those found in nature, and have found application across the electromagnetic and acoustic domains. In 2018, we will run projects ranging from fundamental explorations of physics through to device design. Our students will research a wide range of metastructures spanning 10 orders of scale, from quantum considerations on the atomic scale through to plasmonics and biomimetics and the manufacture of novel composites at the nano-scale. From the control of electron spin (spintronics) and magnonics, to the centimetre and millimetre scale in both acoustics and the RF and microwave regimes.
XM² is interdisciplinary in nature, with projects taking place across Physics and Engineering.
Our core themes are
The first year of the studentship includes a 6-month-project, and a substantial programme of training. Students will choose from a wide range of taught modules, and participate in academic and personal development skills-based workshops, together with creativity events and conference-style meetings. Students will also be expected to disseminate their results to the international community via high-impact publications and international conferences. They will spend time working with our partners at international universities, research institutes, and industry.
The University of Exeter is a top 10 UK university and a member of the elite Russell Group of institutions. The University has committed itself to a substantial expansion in its science base and within Physics and Engineering, over £10m has been spent on infrastructure since 2008 and the fields of electromagnetic materials, nanomaals and graphene have seen 25 new academic staff appointed.


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Atlantis, We Have a Problem
Thanks to Rochelle W. for reminding us that women in space have come a long way.
A Cake Wreck is any cake that is unintentionally sad, silly, creepy, inappropriate - you name it. A Wreck is not necessarily a poorly-made cake; it's simply one I find funny, for any of a number of reasons. Anyone who has ever smeared frosting on a baked good has made a Wreck at one time or another, so I'm not here to vilify decorators: Cake Wrecks is just about finding the funny in unexpected, sugar-filled places.
Now, don't you have a photo you want to send me? ;)
Thanks to Rochelle W. for reminding us that women in space have come a long way.
Cake Wrecks has made me realize just how phallic the space shuttle really is. I never thought about it until Women in Space.
Well, that's the most interesting castle-dong-shuttle I've ever seen. I can honestly say that.
Unicorn romance:
New crest for NASA doubles
as office thank-you.
What says "great job" more
than horny beasts, rock-hard fort?
Blue-on-white action.
wv: otlim
I otlim be going now.
It's diseased. And so much fail...
I really wish I knew what the wreckerator thought when they stepped back and looked at this one.
If I were Rochelle, I wouldn't go near that thing until it had a shower and a 10-day course of antibiotics.
Is anyone else having flash-backs to Disney's "The Little Mermaid" VHS cover?
(yes, i did just date myself :P )
Now THAT'S a rocket! Zing!
(Is that '70s-era funked-out porn guitar I hear in the distance? Wakka-wow, Wakka-wow-wow...)
The bakers of today really need to take their minds out of the gutter. Every other cake resembles poop or unmentionable body parts.
When I saw this cake the song "Rocketman" started playing in my head. (Yes, my brain runs a constant soundtrack.) Then I noticed the add to the left of the wreck was for an upcoming Elton John concert. Weird coincidence. (Yes, I said "upcoming". Get your mind out of the gutter!)
Hmmm..astro...NOT! Though this may explain the inspiration for Jerry Lee Lewis's hit "Great Balls of Fire."
wv-panjufa: A small uninhabited island off the coast of East Africa.
Note to castle thingy:
The Enrichment Center promises to always provide a safe testing environment.
In dangerous testing environments, the Enrichment Center promises to always provide useful advice.
For instance, the floor here will kill you. Try to avoid it."
Castles are pretty phallic, too. All those, erm, spires.
I could make a comment here about that being why girls love them, but that would be kind of ... eeeewwwwwww.
One has to wonder what kind of job that baker thinks Rochelle is doing, that she gets not one but THREE giant phallic symbols on her cake! And a well... splattered?!... full set of equipment on top. Unless... someone is very happy to see her each morning. o.O
But at least today's cake wasn't flesh-coloured, unlike the last women-in-space one. (Oh, I had forgotten that gem)
I have to agree with Fluffy Cow... really, what did they think???
All the wonderful discoveries, and that is how they remember it-sad :(
The black fuzzy (furry?) stuff adds a nice accent to the work.
At least the chocolate chip cookie looks yummy....
The image on that cookie "cake" looks like the setting for the inevitable "Harry Potter" porno spoof... O_o
Wow, I just happened to be wearing my NASA t-shirt today, perfect for the giant dong rocket you're featuring. NASA = Not Another Sexual Astronaut (?)
Sky rockets in flight. Afternoon delight...
Rochelle - On your achievement, a Dong Mahal.
(OMG, I just realized it says Great Job. :x)
Is it just me but the brown blob on the left of the dong/rocket looks like a mini godzilla?
wv: preogi
That's the prefect preogi cake.
Oh, so many possibilities, humor synapses in overload -- ... countdown ... 10 years of??? ... "we have liftoff" ... "KY, or, Frosting? mmmm" ... "to the MOON, sweetheart" ...
Rev W
w.v.comon: speling errirs ar comon on cake wrekcs
You should do an encore posting of the shuttle cake celebrating the women of NASA
hmmm...brings an entirely new definition to the word 'shuttlecock'
I can just imagine the look on the recipient's face... LOL
I love the witty, sly, verbal puns refering to penii on Cakewrecks; but sometimes, I really like it when you just call 'em like you see 'em, too. Thanks for the chortle--I nearly spit my ice cappaccino all over my keyboard!
I'll admit it, my comment today has absolutely nothing to do with the creepy cake today. It is a protest. I will admit it, I am a fan of Wilton on Facebook. Sadly their featured cake/technique today was a baby butt covered with a blanket. They said that it is a trend among baby shower cakes. This "trend" MUST be stopped!!!
Thank you.
Lol....they just showed the shuttle landing again, "the Space Program - stimulating the imagination of a nation like no other." There you go.
at first i was like, why is there a wang on a castle and then i looked at the title and thought, ooohhhhh, space shuttle program.......
@Sharyn- woooooo hooooooooo!!!
-Barbara Anne
wv: (I swear, I couldn't make this up) penogilg
I love it. Simple, short, and HILARIOUS.
(captcha: cundsms... oh dear...)
Lmao that just made me want to scream out loud with laughter but alas it would scare my hubby who is playing Halo: Reach at the moment lol. Oh man I didn't realize how phallic that shuttle looks..scary.
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Yes, @"*#)@*#! You see a flaw in a piece of my patterned Slinky fabric. I'm making Simplicity 4074, the wrap dress. This was *supposed* to be a good dress. It's okay, I'll need a black slip. But still. When I bought this fabric, I thought that snag was a crease in the fabric. It's on several pieces of the dress in different places. I created princess seams on the back and it's on the lower parts of two of the pattern pieces. Now it's on a front side panel.
So what gives? It hasn't been the best week, certainly not the worst, but happy that it's almost over. First that crappy plaid (of which I purchased more last night to cut the front pieces--blech!), then getting rear ended (thank you, everyone, for your kind words), then taking it easy the day of the accident and making this dress, only to find there's that "lovely" snag. It shouldn't be that noticeable with a slip, but still. It has jaded my view on this project from what should have been a pretty decent wrap dress.
So...the positive points:
The good thing is, it it will still be wearable.
Healthwise, other than just a very mildly sore front/side neck muscle which only is a little achy a couple times during the day, my health is absolutely fine. The only time it slightly bothered me yesterday at the gym was doing situps on a steep incline on the incline bench. And when I lifted my head to rest it on my pillow while lying on my side. Other than a few moments here and there, I feel absolutely fine.
The lounge pants? They'll get made. And so what if the stripes are off--they pants are not for me LOL.
In the grand scheme of things, life is good. Don't sweat the small stuff.


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PROGRESS Chapter 48: Bang The Drum
PROGRESS Chapter 48: Bang The Drum
By GARZA
From May 14, 2017
Jim Smallman Intro
- IRS, Hogan, British Bulldog, Stone Cold, and Sting are all on attendance today. Not really. DON'T BE A DICK!
SSS16 Qualifier Match
James Drake vs "Flash" Morgan Webster - 7/10
Getting payback from the sneak attack last week, Webster starts the brawl early during Drake's entrance. Drake was working Webster's jaw all match, the man has a jawbreaker forearm and he wants another victim tonight. Webster's hope spots were short and not many, but they were all big enough that it felt like it was balancing out Drake to him. Finish saw Drake try to use the tights to pin Webster, but ref saw it, while Drake argues with the ref, Webster connects with the knee and Slice Bread for the win. This is James Drake's first loss.
Jinny vs Session Moth Martina - 6.5/10
Jinny tonight has the chance to get some momentum for the Natural Progression Series IV finals. Martina obviously with her super over entrance, if only her wrestling was at par, different song tonight though, so that kinda sucks. Martina instead of streamer treatment, got a glowstick treatment. I can't imagine a better feud other than Moth vs Jinny. Match started with a series of pin attempts while Martina had a beer, no drop was wasted.
Match was mostly Jinny dominating, with Martina having some great comebacks here and there, her style is really fun to watch sometimes, but at the end, Jinny decimated Martina. At one point Jinny choked Martina with a plastic bag, which was a great visual and heat magnet. This ended up being a fun match after all.
London Riots vs Sweet Jesus - 6.5/10
A bit of a boring match personally, everything with Mambo felt like a drag, his comedy is stupid, and his wrestling subpar, a bunch of his moves look terrible. Eaver's parts however were all great, we haven't seen much of him since the Sebastian battle, so fans were hyped about them. Match got good towards the end, but finish was a bit lackluster and it seems like they'll build Sweet Jesus towards a title shot. Davis took off after match without Lynch, but I'm not gonna read too much into that.
Travis Banks vs Trent Seven - 8.5/10
It's just not the same hearing that song without TK Cooper coming out and making out with a picture of Dahlia Black, but hey, it's time for the Kiwi Dragon to breakout! These guys went through a long brawl outside the ring that once they made it to the ring, I had totally forgotten the match hadn't officially started. Banks schooled Seven on what it means to be known as Buzzsaw, and Seven finally lived up to his stable's name, simply said, these guys went in to beat each other out, and oh god, was it amazing! Unfortunately, the finish sucked bad, I have to believe they rematch at SSS16, potentially at the semis.
SSS16 Qualifier Match
"The Professional" Nathan Cruz vs "Rockstar" Spud - 7.5/10
You think we can get another PROGRESS logo in Spud's attire? Or maybe some other company to sponsor his entrance song, because this new one kinda sucks. This is a Spud match, with the exception of his wars with Swoggle, there is only one formula for Spud matches, that is getting his ass kicked for most of the match, some flurries of hope spread around, and one final babyface fire dance that may or may not get him a win. In this particular case, that was a nay, Cruz killed Spud with the reverse showstolen , but to Spud's defense, he was battling injured ribs that he injured, also worth mentioning that Spud kicked out of several showstolens before finally failing to kick out again.
Match was actually great, formula or not, Spud is a great babyface underdog.
Tyler Bate vs "King of Bros" Matt Riddle - 9/10
Double champ vs double champ tonight, and none of the titles are on the line. WWE UK title got booed at the mention of it, then they did a yay vs boo between Atlas and WWE UK titles. Match started with Riddle dominating Bate like a kid until Bate hit a tope suicida after dodging Riddle, but after that small heat segment, it was all back and forward between them. I don't know what they were trying to achieve here, Tyler Bate, the heel, kicked out of all the babyface's signature moves, to the point where he had that 'He can't believe it' look in his face, the one you usually leave for the heels. I question the planning of this match, but that aside, the wrestling was amazing. Finished sucked because Trent Seven ran in for the DQ and we never saw Riddle kick out of any of Bate's moves. Expect Chosen Bros vs British Strong Style soon.
Tyler Bate is a great little cocky heel, but a lot of his wrestling stuff is really babyface-ish, specially considering his size, he's starting to reach Young Bucks heat with half of the fans cheering for you, and the other half hoping you die.
PROGRESS Championship 3-way Match
Mark Haskins vs Mark Andrews vs Pete Dunne (C) - 9.5/10
Talking about song changes that suck but I'll end up getting accustomed to, Mark Haskins song change sucked. So we have two guys that want to kill Dunne and take his title, but the rivalry between them to see who should get a chance to do it, has risen so much that the intensity between then has reached Dunne hate levels.
Match started at 100 mph. It started mostly with one on one pairings, but later on transitioned into a full out 3-way dance inside the ring. Match saw many many 2-on-1 spots, 1-on-2 spots, dives, and submissions. For a second time in a row, finish saw BSS come out to interfere, this time they distracted the ref enough for Haskins to drop the double submission he had on, making an opening for Dunne to low blow him and make enough time for him to hit the Pumphandle Slam on Andrews for the win. Tons of heat.
- BSS celebrate in the ring when Matt Riddle comes out to fight them all, but is easily defeated. Dunne says that everything belongs to him and there is no one left that can take on BSS, then suddenly...CC Fucking K! Mondai Lykos and Chris Brookes AKA CCK come out and take out BSS making them run away. Huge Pop!
It was a good show overall, the bad matches were at least entertaining, and the good matches were amazing. Something I do have to say is that the booking of BSS tonight was really annoying, sure it's heat and all, but three big matches were BSS screwed the top babyfaces of PROGRESS, it really wears you down. Banks was coming in tonight for the first time as his own man, this really needed to be a night where he stood tall, but they went for the screw finish. It better be opposite momentum and he better win SSS16.
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Timeline: History of the WWF 1963-1969 with Bruno Sammartino


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Knowledge into useful tech
Find out more about our laboratory, from our research and projects to our facilities.
The Nano-Micro Fabrication and Reliability Laboratory was established to address the increasing need to transfer knowledge of micro-systems and devices into useful technology.
In recent years there have been dramatic developments in the scientific understanding of the materials, manufacturing and operation of devices at the micro and nano scales. MEMS and other micro and nano devices are finding increased applications in a variety of industrial and medical fields. Some examples are accelerometers for vehicle airbags, micro heat exchangers for cooling of electronic circuits, reactors for separating biological cells, blood analysers, ink-jet printing, diode lasers and high-frequency fluidic control devices.
The potential worldwide market for such micro-systems and devices is estimated to be USD 30 billion. Rapid advances are also being made in the understanding and fabrication of materials and devices at the nano scale with potential applications in many fields of industry and medicine.
Find out more about our laboratory, from our research and projects to our facilities.
We focus on the fabrication, characterisation and application of high-k dielectrics and graphene materials amongst other research.
Staff in the laboratory have conducted a number of academic research projects.
There are more than 100 items of major equipment in the Department of Electrical and Electronic Engineering's research lab, which is accessible to our staff.


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Also found in: Dictionary, Wikipedia.
Chiclayo(chēklä`yō), city (1993 pop. 240,050), capital of Lambayeque dept., NW Peru. On the coastal desert between the Andes and the Pacific, Chiclayo may go years at a time with no rainfall. However, by utilizing short Andean streams for irrigation, Chiclayo raises considerable sugarcane, some cotton, and a major part of the country's rice. A major commercial center of the Peruvian north, it is located on the Pan-American Highway and has road and rail connections to Eten and Pimentel.
a city in northeastern Peru; the capital of Lambayeque Department. Located on the Pan-American Highway. Population, 189,700 (1972).
Chiclayo is connected by railroad with the ports of Pimentel and Puerto Eten. The city's food-processing industries manufacture dry and condensed milk, sugar, and processed rice. The city also has leather and textile industries and enterprises for the production of building materials. It is the center of an agricultural region that produces rice and sugarcane. A university is located in Chiclayo.
a city in NW Peru. Pop.: 434 000 (2005 est.)


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Green Cleaning
What Makes Green Cleaning Services in San Diego?
You may notice that some professional cleaning companies state that they are "green friendly" or green technology based. What does this mean? Green cleaning services in San Diego are companies that use green-friendly (or environmentally-friendly) products. This usually means that all cleaning solutions used are 100% natural. This does not merely imply non-toxic materials, but organic ones. This is the true meaning of "green", nothing harmful and usually natural.
What does a green-friendly cleaning solution really mean?
Most of the cleaning solutions used by professional green cleaning services in San Diego are water-based or at least free from man-made chemicals, which are, of course, the number one cause of pollution. Most companies buy their solutions from other manufacturers. For example, Sierra Natural Cleaners is a popular brand producer for natural cleaners.
Breathe better and live longer…
Oh, how to express the benefits of a green-friendly business. If only all professional companies used green practices, we could have confidence that our grandchildren would have a safe and clean environment to grow up in. We would breathe better, feel healthier and live longer. All green technologies and products are for the welfare of our planet.
Green is not just a product but a way of life
All worldwide issues aside, locally and individually speaking, using green cleaning services in San Diego can actually protect people from allergy attacks and general sickness and flulike symptoms. Besides natural products, green business also means using recyclable parts and tools, and even toiletries and paper towels. Natural products and techniques work just as well—if not better—than chemical-based degreasers and soaps. It is a win-win situation for everyone involved and the best part is, the cost difference is not that much.
Help preserve natural resources and promote better health in your own life with green cleaning services in San Diego!


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01 December 2006
LaSalle Investment Management's UK ventures fund has bought Reading Bridge House in Reading for £30m from Exemplar Properties.
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The new feature film documentary The Beatles in India, diretced by two time Emmy award winning producer-director Paul Saltzman, is set to be released worldwide in Autumn 2018.
In 1968, with the eyes of the world upon them, The Beatles travelled to Rishikesh, India, to study Transcendental Meditation with Maharishi Mahesh Yogi in a remote ashram on the banks of the sacred river Ganges. Those few short weeks became one of the most prolific and creative periods of their lives.
Also present at the ashram was a young Canadian filmmaker named Paul Saltzman, there to heal a recently broken heart while seeking his own path to understanding and enlightenment. In the informal and relaxed atmosphere, he captured some of the most famous and intimate photographic portraits of The Beatles ever taken.
Source: Gary Collinson/flickeringmyth.com


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Clatsop County, Oregon District Attorney since 1994.
Active Democrat. Served as a delegate to the 1996 Democratic National Convention. Work to retain thoughtful Democrats to local, statewide and national offices.
A frequent speaker at universities and professional meetings, and a frequent guest on national and local radio and television programs, discussing the criminal justice system and the nature of the relationship between popular culture, the media and the law.
Educated in Oregon except for high school in Claremont, California. Graduate of the University of Oregon's Honors College and Law School. Worked in the Lane County (Eugene) DA's office during law school.
Returned to Claremont for four years, working first as a newspaper reporter in Los Angeles, then as a speechwriter to California's Attorney General John Van de Kamp.
Returned to Lincoln County (Newport), Oregon in 1985. Chief Deputy District Attorney 1988-89.
Making money seemed like a good idea. Moved to Eugene and worked 18 months as a criminal defense lawyer, representing three defendants in capital murder cases. It was horrible.
Was hired as chief deputy DA in Mike Dugan's office in Deschutes County (Bend), in 1990, and became Chief Deputy in 1992.
In 1994, was appointed by Governor Barbara Roberts to succeed Clatsop County DA Julie Leonhardt after she was recalled, convicted and disbarred. Was elected by the voters later that year and have lived in an old house on the north slope of Astoria ever since.
An active Rotarian (and Paul Harris Fellow) since 1994. Recently returned to a bi-monthly slot as an Evening Jazz DJ on Astoria's community radio station, KMUN. (First and third Mondays, 6-8pm.) Continuing this summer a 20th year of occasional appearances as the Cowardly Sheriff in the Astor Street Opry Company's play "Shanghaied in Astoria."
Married since 1996 to Cindy Price, editor extraordinaire, who when asked what she does says, "I make things," including this website.
Married since 1996 to Cindy Price, editor extraordinaire, who when asked what she does says, "I make things," including this website.


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Open letter to London city council
Mr Fontana, and the citizens elected to City council. Thank you for the opportunity to speak to this forum. I Intentionally do not address you by your titles but as members of this community as a reminder of who you represent. First I would like to complement you on your ability last budget to maintain… Read More Open letter to London city council


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Saurer soutache system with application heads
Saurer's innovative SoutacheHead enables fully flexible embroidering of unique designs while maintaining a perfect view of all embroidering positions.
Intelligence on each embroidering position
The secret behind our unique precision lies in the innovative
application heads. Each embroidering position has its own motor which is individually positioned and controlled. The intelligent and motor-driven head movement guarantees synchronous motion and consistent Saurer embroidering quality from the first to the last needle.
- Production speed of up to 500 rpm
- Individual monitoring of each head guarantees precision over the entire embroidery length
- Free choice of repeat (12/4 and up)
- Easy and very fast changeover between repeats as well as easy and fast exchange of application heads
- Processes cords up to 4 mm and ribbons from 6 to 8 mm
- Integrated mending function
- In normal embroidering mode, the full embroidery height is available to you
- Working at embroidering positions without any difficulties, thanks to the generous space provided


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Shipping cost
|china post
|free shipping,will arrived at 10-15 working days,can be tracked
Refund policy
|If you are not satisfied when you receipt our item,Please contact us in 2 week,we are can discuss instead item or refund. If Item is unworn and don\'t breakage. you can get wholly refund (not include the return shipping fee).
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|We are deal with in processing of the jewelry and selling trade in China,we have abundant jewelry experience,we can offer different style and different types of jewelry give you,if you can offer design of the jewelry to me,we can customize the jewelry design suitable for you to give to you.If you order our items, we will contact you in 24 hours.If you order many items,we would like to combine shipping costs to save you money. We offer excellent packaging to ensure those items you order can arrive you safely.When the payment is received,we will send off the items to you in two work days.Please note the shipping duration,if the package has not been received on time,please feel free to let us know.We\'ll track the package from our post office,and let you know the package number to track it from your post office.If the package lost,we\'ll refund you.


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|Sesame Street
|The gang goes camping
|Air date
|January 13, 1977
|Season 8 (1976-1977)
This episode guide has been compiled from materials at the CTW Archives. ⓘ
- The campers construct a tent.
- Susan explains how to build a campfire.
- After the campfire is built, the gang tells a campfire story full of B words.
- While trying to sleep, Gordon and Susan have an argument.
- It starts raining, so all the campers take shelter in Gordon and Susan's tent.
- Grover the Elevator Operator: Only five people are allowed on the elevator at one time, so Grover counts out five passengers. But then he's not allowed on the elevator himself; he would make six. (First: Episode 0462)
- The Mad Painter paints a number 2 on a sailboat, enraging the owner. (EKA: Episode 0353)
- Cartoon: A man tries to figure out where a squeaky sound is coming from; it turns out to be a giant's squeaky shoes. "Surprise!" (EKA: Episode 0810)
- Film: Footage of a city neighborhood; includes shots of a sno-cone vendor. (EKA: Episode 0695)
- Cartoon: A boxer explains the letter "B" and the purpose of boxing gloves. (First: Episode 0006)
- Film: Footage of a road being constructed intercuts with clips of kids digging a road in the sand on the beach. (EKA: Episode 0310)
- Cartoon: Four people cooperate building a table. (EKA: Episode 0772)
- Film: "There Once Was a Hand" — Skip Hinnant narrates the story of an unhappy hand that wishes he could make a noise. (First: Episode 0007)
- Cartoon: A red PELI and an explosive GRO form the word PELIGRO. (EKA: Episode 0811)
- Film: What do birds eat? (EKA: Episode 0514)
- Maria and the Kids put together a letter B that's broken in several parts. (EKA: Episode 0930)
- Film: Lizard! (EKA: Episode 0820)
- This episode was later repeated in Season 10 as Episode 1236.
|Previous episode:
|Next episode:
|Episode 0958
|Episode 0960


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flying lessons are drawing to a close…
wait a minute… are they??
or are the lessons just beginning??
we are all in different stages of this flight
called the creative life….
some were already soaring pretty high
a little boost was all they needed
to say good-bye to gravity
burst the bounds of the atmosphere
now gliding effortlessly with no end in sight
others are moving up to join them
but their flight is taxing
there's a lot of flapping going on
they soar up
then dip back down
it's an ardous journey
still others are having trouble leaving the nest
they've taken a few cautious test flights
they're hopeful….
but flying is a scary prospect after all
and then there are those
who haven't yet left the ground
they look up and what do they see?
everyone else is way ahead
gliding, rising, soaring
and never seeming to touch the ground
but look…..
some of the highest flyers
are dipping back down
they're coming all the way back
and they're helping those
who haven't yet mastered the art of flight
they're guiding them
and showing them how it's done
....little by little...
everyone eventually makes it off the ground
some higher than others
but it's ok
everyone finds their own flight plan
it's an ongoing work in progress
but it's a beautiful sight!!


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Last weekend, I was bestowed with the hands of death – everything I touched was maimed or destroyed. At times like these, it's best to turn to the professionals. Or in my case, return to the professionals, so a handful of my prints are back at Digital Silver Imaging for mounting and reprinting. DSI is one of the best companies I've had the pleasure to work with, and I just had to give them a shout out. Their work is phenomenal and they've come through for me in a pinch more than once.
But on to other things!
Back in June, which apparently was only a month ago, I had the pleasure of working with another great company in the area, Nara. They just launched a public beta version of their website, which BostInno described as a combination of Yelp and Pandora. Users input their three favorite restaurants, and Nara will provide suggestions of restaurants that match the users preferences. It's a really fun site to use, and I recommend getting in on the ground floor with the website – looks like Nara is also planning to expand to travel and leisure as well.
My work was far more simple than building a search engine though – I was commissioned to shoot headshots of Tom Copeman, the CEO, and Nathan Wilson, the CTO. They were an absolute pleasure to work with and I look forward to seeing their company grow! Check out a few of the shots below:


text: HomeTulip darwin hibrid world FavTulip darwin hibrid world Fav Emaknya Benjamin br. Silaen October 15, 2015 No Comments Ad Blocker DetectedOur website is made possible by displaying online advertisements to our visitors. Please consider supporting us by disabling your ad blocker.Refresh Tulip darwin hibrid world Fav Advertisements Prev ArticleAbout The Author Emaknya Benjamin br. SilaenHi! I'm Nella a Bataknese. I currently live in Germany. However, I'm originally from Jakarta, Indonesia. I'm a full-time-stay-at-home-mom of two. I have a lot of passions for gardening. Leave a Reply Cancel reply This site uses Akismet to reduce spam. Learn how your comment data is processed.


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- Short Report
- Open Access
Effect of the vitamin B12-binding protein haptocorrin present in human milk on a panel of commensal and pathogenic bacteria
- Henrik R Jensen†1,
- Martin F Laursen†1,
- Dorte L Lildballe2,
- Jens B Andersen1,
- Ebba Nexø2 and
- Tine R Licht1Email author
© Licht et al; licensee BioMed Central Ltd. 2011
- Received: 10 March 2011
- Accepted: 21 June 2011
- Published: 21 June 2011
Haptocorrin is a vitamin B12-binding protein present in high amounts in different body fluids including human milk. Haptocorrin has previously been shown to inhibit the growth of specific E. coli strains, and the aim of the present study was to elucidate whether the antibacterial properties of this protein may exert a general defense against pathogens and/or affect the composition of the developing microbiota in the gastrointestinal tracts of breastfed infants.
The present work was the first systematic study of the effect of haptocorrin on bacterial growth, and included 34 commensal and pathogenic bacteria to which infants are likely to be exposed. Well-diffusion assays addressing antibacterial effects were performed with human milk, haptocorrin-free human milk, porcine holo-haptocorrin (saturated with B-12) and human apo-haptocorrin (unsaturated). Human milk inhibited the growth of S. thermophilus and the pathogenic strains L. monocytogenes LO28, L. monocytogenes 4446 and L. monocytogenes 7291, but the inhibition could not be ascribed to haptocorrin. Human apo-haptocorrin inhibited the growth of only a single bacterial strain (Bifidobacterium breve), while porcine holo-haptocorrin did not show any inhibitory effect.
Our results suggest that haptocorrin does not have a general antibacterial activity, and thereby contradict the existing hypothesis implicating such an effect. The study contributes to the knowledge on the potential impact of breastfeeding on the establishment of a healthy microbiota in infants.
- Lactobacillus
- Human Milk
- Brain Heart Infusion
- Bacteriocin Activity
- Breastfed Infant
Background and hypothesis
Haptocorrin (previously named transcobalamin I and III, and R-binder) is a glycoprotein, which is present in human milk in relatively large amounts [1, 2]. It is characterized by its ability to bind vitamin B12 and other corrinoids [3], and exists in two forms in human milk; apo-haptocorrin, which is unsaturated with vitamin B12, and holo-haptocorrin, which is saturated with vitamin B12 [4]. Human milk contains a much higher concentration of apo-haptocorrin than of holo-haptocorrin [1, 2], but the role for the high amount of apo-haptocorrin in human milk is unknown. Furthermore, the function of haptocorrin in different body fluids, i.e. secreted from exocrine glands or released by erythrocytes and leucocytes, remains unknown. However, the current concept is that haptocorrin in adults is responsible for transporting dietary vitamin B12 through the stomach to the intestine. Haptocorrin is believed to be degraded by the intestinal enzymes and the vitamin B12 released from the protein is subsequently bound to intrinsic factor and absorbed in the distal part of ileum [5]. It has been proposed that haptocorrin contributes to the transport and absorption of vitamin B12 in infants while the gastric secretion of IF is not sufficiently developed [6]. On the other hand, the high amount of apo-haptocorrin in human milk has lead to suggestions that haptocorrin, by withholding vitamin B12, exhibits antimicrobial effects in the intestine and thereby potentially protects the infant against pathogenic bacteria [1, 7]. Additionally, we speculate that an antibacterial (protective) function of haptocorrin could explain its presence in various body fluids.
The possible effect of haptocorrin on bacteria has been discussed since the late 1970's [1, 7–9]. However, only a very limited number of studies addressing such an effect have been performed, and these studies have focused on the effect of haptocorrin on E. coli[8–10]. Samson et al demonstrated that apo-haptocorrin, derived from human milk, inhibits the growth of the vitamin B12-dependent E. coli N.C.I.B. 8134 [8]. This effect was shown to be diminished by pretreatment with trypsin [9]. The gastrointestinal tract of infants differs from the adult gut in terms of higher pH and lower trypsin activity [11–14], thus haptocorrin may still be functional in the upper part of the intestine during infancy. Indeed, it has been demonstrated that porcine haptocorrin is not degraded in vitro when subjected to an environment simulating the gastrointestinal tract of infants [10].
Previous studies have shown that both apo-and holo-haptocorrin inhibits the growth of a single enteropathogenic E. coli O127 strain (EPEC) [10], thereby suggesting that the haptocorrin moity rather than its ability to bind cobalamin inhibits the growth of pathogens [15]. However, to date no study has systematically investigated the effect of haptocorrin (saturated or unsaturated) on a panel of bacteria.
Recently, the importance of the first colonizing bacteria in infants for the development of a healthy immune system has been highlighted [16, 17], and it is known that the microbiota of breastfed infants differ from that of formula fed infants [18]. We hypothesized that haptocorrin from human milk, by inhibiting the growth of certain bacteria and thereby favoring the growth of other bacteria, may influence the initial colonization in breastfed infants. We therefore performed the first systematical study of the effect of haptocorrin on a panel of 34 commensal and pathogenic bacteria to which infants may be exposed, and conclude that human unsaturated haptocorrin had no general antibacterial effect, but was seen to inhibit the growth of Bifidobacterium breve, which is commonly found in the intestinal tract of neonates.
Materials and methods
Biochemical methods
The concentration of vitamin B12, in BHI and MRS media, was analyzed employing the Cobas 6000 E immunoassay system and the analytical kit supplied by the manufacturer (Roche). Unsaturated vitamin B12 binding capacity (UB12BC) in BHI and MRS was measured as described previously [19]. The vitamin B12 content and the UB12BC of Clostridium difficile media were not measured due to lack of the liquid form of the specific agar media used in the well-diffusion assay.
Human milk
Human donor milk, collected at Randers Hospital, Denmark was centrifuged at 10,000 g, 4°C, 15 min. It contained 70 nM haptocorrin and 0.85 nM cobalamin (holo-haptocorrin).
Haptocorrin-free human milk was prepared by affinity purification using polyclonal antibodies against human haptocorrin [3] covalently linked to protein A/G agarose according to instructions of the manufacturer (Santa Cruz). > 99.9% of the haptocorrin was removed, as controlled by ELISA and by measurement of UB12BC. Milk with and without haptocorrin was used without further dilution. The samples were stored at -20°C until use.
Porcine holo-haptocorrin (saturated with vitamin B12) purified from gastric extracts [20] was employed. The protein was diluted in 5 mM phosphate buffer pH 7 to a final concentration of 250 nM. Human haptocorrin unsaturated with vitamin B12 was purified from human donor milk essentially as described previously [21]. The milk originated from a complete lactation from one donor. In brief, 100 mL milk was absorbed to 12 mL vitamin B12-sepharose at room temperature with gentle agitation over night. Subsequently, the B12-sepharose was applied to a glass column. After washing with 500 mL 50 mM Tris-HCl pH 7, 150 mL 50 mM TrisHCl with 0.5 M NaCl pH 7, and 100 mL H2O, the absorbed haptocorrin was eluted with 3 × 20 mL 5 M guanidine-HCl each eluted during 20-30 min. The eluates were pooled and dialysed against excess demineralised H2O for > 48 h at 4°C. After lyophilization, the protein was redissolved in 5 mM phosphate buffer pH 7 to a final haptocorrin concentration of 250 nM as determined by ELISA, and as judged by UB12BC analysis, > 97% occurred as unsaturated haptocorrin. All haptocorrin samples were filter sterilized (0.22 μm) and stored at -20°C prior to use.
Bacterial strains and growth media
Effect of human milk, haptocorrin free human milk, apo-haptocorrin and holo-haptocorrin on the growth of selected bacterial strains
Strain and source [reference] a, b, c, d
Human milk
Haptocorrin free human milkg
Bifidobacterium breve
DSM no 20091
Bifidobacterium infantis
DSM no 20088
Bifidobacterium bifidum
DSM no 20215
Lactobacillus acidophilus
DSM no 20079
Lactobacillus johnsonii
DSM no 10533
Clostridium difficile
DSM no 1296
Staphylococcus epidermidis
DSM no 20044
Staphylococcus aureus
Enterococcus faecalis
Enterococcus faecium
Streptococcus thermophilus
E2; Tosi et alf
Streptococcus agalactiae
Escherichia coli
Escherichia coli
O157, wild typee
Escherichia coli
O103:H2, wild typee
Escherichia coli
O26:H11, wild typee
Escherichia coli
O145:H-, wild typee
Escherichia coli
O26:H-, wild typee
Escherichia coli
O126:H20, wild typee
Escherichia coli
O128ab:H-, wild typee
Escherichia coli
O128ab:H2 (1), wild typee
Escherichia coli
O128ab:H2 (2), wild typee
Escherichia coli
O111:H-, wild typee
Escherichia coli
O127a:H-; DSM no 8702
Listeria monocytogenes
Listeria monocytogenes
4446; Larsen et al, 2002f
Listeria monocytogenes
7291; Larsen et al, 2002f
Salmonella Infantis
(1), wild typee
Salmonella Infantis
(2), wild typee
(1), wild typee
(2), wild typee
Salmonella Typhimurium
Salmonella Typhimurium
Salmonella Typhimurium
Well-diffusion assays
The applied well-diffusion assay, which previously has proven useful to screen for bacteriocin activity [27], is suitable when only small amounts of samples are available, and sufficiently sensitive to reveal effects of biological relevance. The assay was a modification of the procedure described by Fuente-Salcido et al.
BHI, MRS or specific Clostridium difficile media with agar (2%) was melted and tempered to 50°C. One single cell colony was suspended in 1 mL of the corresponding bouillon (MRS, BHI or a specific Clostridium difficile liquid media (CM81)) and mixed with 400 mL agar media, which was plated in 20 agar plates and allowed to gelatinize. A well, 7 mm in diameter, was made in each agar plate and 20 μL of test sample (human milk, haptocorrin-free human milk, porcine holo-haptocorrin and human apo-haptocorrin), 20 μg mL-1 erythromycin (positive control for inhibition) or 5 mM phosphate buffer pH 7 (negative control for inhibition) was added into the well. Only strains inhibited by human milk were subsequently tested with haptocorrin-free human milk. Plates were incubated as described above observing possible inhibition zones. An inhibition zone of minimally 5 mm was defined as a positive inhibitory effect. Assays were performed in triplicates.
Media characteristics
BHI media contained 0.13 nM UB12BC and 0.05 nM vitamin B12, whereas the MRS media contained 0.12 nM UB12BC and 0.44 nM vitamin B12, which is concentrations well below the concentration of added haptocorrin (250 nM). Therefore, it seems highly unlikely that the vitamin B12 content and the UB12BC of the media could influence our results, respectively by saturating apo-haptocorrin or taking over haptocorrin's role in vitamin B12 binding.
Effect of human milk with and without haptocorrin
The positive control (erythromycin) and the negative control (phosphate buffer) gave the expected results for all 34 strains tested; the positive control caused an inhibition zone around the wells, while the negative control did not.
Human milk inhibited growth of S. thermophilus and the pathogenic strains L. monocytogenes L028, L. monocytogenes 4446 and L. monocytogenes 7291 (Table 1). However, this inhibition was not caused by haptocorrin, since haptocorrin free human milk gave the same result.
In some cases, a visible increased density of the bacteria appeared around the wells, when compared to the growth around the wells only containing buffer. This is indicated as 'increased growth' in Table 1, and was observed around the wells after addition of human milk for Bifidobacterium breve, B. bifidum, Lactobacillus acidophilus, L. johnsonii, Staphylococcus epidermidis, E. coli O103:H2, E. coli O145:H - , E. coli O128ab:H - , E. coli 128ab:H2(1), E. coli 128ab:H2(2), E. coli O111:H - , E. coli O127a:H - , Salmonella Enteritidis (1) and S. Enteritidis (2).
Effect of saturated and unsaturated haptocorrin
Due to limited amounts of human milk derived haptocorrin, available porcine haptocorrin was employed for studies on the effect of the saturated form of the protein. Addition of porcine holo-haptocorrin in the well-diffusion assay showed no inhibition of any of the bacteria. Unsaturated human haptocorrin caused an inhibition for B. breve, but not for any of the other bacteria.
In the well-diffusion assay originally developed for assessment of bacteriocin activity, [27], human milk inhibited growth of S. thermophilus and the pathogenic strains L. monocytogenes LO28, L. monocytogenes 4446 and L. monocytogenes 7291. For the latter three, this is consistent with the findings of Lopez-Exposito and coworkers, who ascribed the inhibitory effect to the lysozyme in human milk, possibly in combination with lactoferrin/immunoglobulin A [28]. We were surprised to see an inhibitory effect of human milk on S. thermophilus, as this species is widely used in the dairy industry [29], but also this is likely to be attributed to lysozyme/lactoferrin/immunoglobulin A or other antibacterial proteins in human milk [30]. Our experiments with haptocorrin-free human milk revealed that the observed inhibitory effect of human milk on the mentioned bacteria was not caused by haptocorrin, because similar inhibition zones were observed for haptocorrin-free human milk as for human milk with haptocorrin. In addition, no inhibitory effect was observed with either purified porcine holo-haptocorrin or with human apo-haptocorrin on the given strains.
Human milk caused a higher density of certain bifidobacteria, lactobacilli, staphylococci, E. coli and Salmonella strains around the wells. It is well known that lactic acid bacteria and E. coli strains can ferment lactose present in human milk [31]. More surprisingly, increased growth was observed for two (non-lactose fermenting) Salmonella Enteritidis strains, which may be ascribed to the oligosaccharide content in human milk.
No observable inhibitory effect of porcine holo-haptocorrin on any of the tested bacterial strains was observed. A study by Adkins & Lönnerdal has shown inhibitory effect of porcine apo-and holo-haptocorrin on the growth of a specific enteropathogenic E. coli (EPEC) strain [10]. However, our results suggest that such an inhibitory effect is not general towards all EPEC strains. We tested 11 EPEC strains with porcine holo-haptocorrin and did not observe inhibition of the proliferation of any of these bacteria.
It has previously been suggested that the inhibitory effect of human haptocorrin on bacterial growth depends on saturation with vitamin B12, and that only apo-haptocorrin exhibits inhibitory effects [8]. The inhibitory effect of haptocorrin on bacteria may therefore be a matter of absence of vitamin B12 dependence of the strains.
Unsaturated haptocorrin purified from human milk inhibited the growth of a single strain (B. breve) of the 34 included strains. This was highly surprising, since B. breve is known to be one of the most common bacteria in the gut of breastfed infants [32]. The inhibitory effect was not observed with saturated porcine haptocorrin. This implies that the degree of saturation affects the inhibitory activity of haptocorrin on bacteria, as suggested by Samson et al[8]. Alternatively, the origin of protein isolation (human or porcine) may influence the effect of haptocorrin since the amino acid sequence and the degree of glycosylation varies depending on isolation species [33], but further studies are required to elucidate this.
In spite of the observed effect on B. breve, our results suggest that haptocorrin does not have a general antibacterial effect, and thereby contradict the existing hypothesis implicating such an effect [1, 7, 10, 15]. Therefore, we find it unlikely that haptocorrin present in human milk through inhibitory properties alter the composition of the first colonizing bacteria in the intestine of breastfed infants.
Acknowledgements and Funding
The work was supported by grants from the Danish Medical Research Council and the Lundbeck Foundation. The technical help from Anna Lisa Christensen and Bodil Madsen is warmly acknowledged.
Authors' Affiliations
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This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Exploring Social Care Policy and Provision
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Exploring Social Care Policy and Provision Defining poverty is not easy and there are several reasons for it and again in different countries levels of poverty differ depending on culture. Dallos, R and McLaughlin, E describes one reason for poverty being 'the welfare state, and calls for fundamental changes in its operation'. I will be exploring how poverty and the welfare state are linked how it has emerged and what has been done to help eradicate this for those immersed in this lifestyle. One of the ways people can be helped and poverty can be targeted is though through welfare rights Bateman, N (2005). I will look at the role of the welfare rights unit where I had my first practice placement and will look at their role in the continuous struggle against poverty. 'Welfare rights practice is one way of empowering and protecting people living in poverty' Bateman, N cited in Blackwells (2002). The placement I was on was the welfare rights unit mainly offers service to those already accessing social services, and consists of the adult population. The clients are supported through Care Management. The aims of the welfare (welfare rights has many aims) rights unit is to assist on the take up of benefits as per their policies which are driven through the National Health Service, (NHS). ...read more.
When Labour government first came into power in 1997 one of the key issues targeted by Tony Blair was the welfare state and the creation of a new modern welfare state and to reform the system as it is due to the size of expenditure which at that point in 1995 was about 95 billion pounds 'labour first raised the idea of a more personalised benefit system when in opposition. The gateway idea will reinforce the government's philosophy there should be work for those who can and security for those who cannot work' www.news.bbc.uk/1/hi/uk_politics. Labour wanted the reform bill to look at all agencies working together to help those who can to claim their benefits and exact entitlement and to support those into getting work or for those who are disabled and on incapacity to support in getting work and via choices and training. Today the government has managed to provide some support to people but it just does not seem enough. Labours last few years has according to labour 'created low inflation, steady growth and high unemployment' www.news.bbc.uk/1/hi/uk_politics. Labour's new economic to move more people from welfare to work, the savings on unemployment benefits go towards investing more in education but is this pledge enough can it be sustained . Labour has several pledges but does not tackle those who are really in poverty how can thay afford to buy their own home as this is one of the targets when they can barely live. ...read more.
Reference Acheson, D (1998) 'Independent Inquiry into Inequalities in Health Report' The Stationery Office Banks, S (2002) 'Ethics and Values in Social Work' 2nd Ed PALGRAVE Hampshire Bateman, N (2000) 'Advocacy Skills for Health and Social Care Professionals' Jessica Kingsley Publishers Ltd London Beveridge, J (1954) 'Beveridge and His Plan' for London Hodder and Stoughton by T and A Constable Ltd Publishers London Coulshed, V and Orme, J (1998) 'Social Work Practice an introduction' 3rd Ed published by PALGRAVE Hampshire Dallos, R and McLaughlin, E (ed.) (1999) 'Social Problems and the Family' SAGE publications London Davies, M (ed.) (2002) 'The Blackwell Companion to Social Work' 2nd Ed Blackwell publishing Franklin, J (1998) 'Social Policy and Social Justice' Blackwell Publishers General Social Care Council (2002) 'Codes Of Practice' published by General Social Care Council London Kallen, E (2004) 'Social Inequality and Social Injustice - A Human Rights Perspective' published by PALGRAVE MACMILLAN Hampshire Langan, M (ed.) (1998) 'Welfare; needs rights and risks' published by Routledge London Lishman, J (1994) 'Communication in Social Work' published by PALGRAVE Hampshire McLeod, E and Bywaters, P (2000) 'Social work, Health and Inequality' published by Routledge London Pierson, J and Thomas, N (ed.)(2002) 'Collins dictionary of Social work' HarperCollins publisher Glasgow [Online] Available at: http://www.NHS Care in the Community Act 1990 (accessed on 06 April 2005) [Online] Available at: http: www.labour.org. ...read more.
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Friday, 1 August 2014
Love The Little Things #3
7 comments:
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I decided to submit another lesson before lights out! In this long tutorial, you'll be learning "how to draw a realistic rose", step by step. I've probably got tons of roses on this site, it's kinda confusing! There's a cartoon rose featured in the DragoArt's Top 50, so if you're interested, check it out! This tut is basically long, realistic, and a bit more difficult for those novice artists out there. I've included some helpful tips that will aid on drawing roses a bit more realistically. Practicing in key, so is knowledge and resources. Always be on the lookout for researching on what you're drawing. To draw a rose in it's realistic form, you'll have to observe photographs of roses and how they're built. This drawing on the other hand, was drawn a bit more simpler for the artists that will have a bit of a hassle tackling this on. Anywho, I'm very tired and have to flee on! Thanks for viewing and enjoy learning to "draw realistic roses". Peace out everyone!
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One of my favorite films by one of my favorite filmmakers, is getting the Criterion Collection treatment this summer. Akira Kurosawa's epic crime story, High and Low (1963), will be released in July with a typically impressive list of extras, including:
– New, restored high-definition digital transfer, with newly restored original four-track surround sound
– New audio commentary by Akira Kurosawa scholar Stephen Prince
– A 37-minute documentary on the making of High and Low, created as part of the Toho Masterworks series Akira Kurosawa: It Is Wonderful to Create
– Rare archival interview with Toshiro Mifune
– New video interview with actor Tsutomu Yamazaki, who plays the kidnapper
– Theatrical trailers from Japan and the U.S.
– New and improved English subtitle translation
– PLUS: A booklet featuring a new essay by critic Geoffrey O'Brien and a reprinted essay by Japanese film scholar Donald Richie
If you've never seen this film, you're missing out on one of the great cops-and-kidnappers achievements ever put to celluloid. It's a police procedural at its core, but also an artful look at class in 1960s Japan. So, you can skip the minor DVD editions out now, and get ready for great things this summer.
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