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About Us
For just over four decades, Ted Weiss and his organization have been able to successfully build and establish premier office buildings across Long Island. The strongest attribute of T. Weiss Realty Corp. is its full service characteristic, which includes financing, development, construction, marketing and management, all under one roof. This remarkable level of service is made possible by a strong management team.
Ted Weiss is the Chief Executive Officer of T. Weiss Realty Corporation ("TWRC"), a Long Island based real estate development company. Ted founded the company in 1969, and has since concentrated on the conversion of light industrial properties into Class A office space across the island. Over the years, Ted has overseen the development, leasing and management of over one million square feet of commercial property throughout Long Island. Since the company's inception, Ted has focused on providing TWRC tenants with not only high quality office space, but also numerous value-added services that support the success and growth of their businesses. He is the visionary behind the progressive amenities and style of service that can be found in all TWRC properties.
Consistent with TWRC's pledge to fulfill its tenants' every need, Ted established TOTUS Office Solutions and Conference and Meeting Centers of Long Island; both of which he still serves as President. The two companies have achieved a significant presence in the Melville business district.
Ted is committed to the Long Island community. He is a member of the Long Island Association (LIA), Melville Chamber of Commerce and the Commercial Industrial Brokers Society (CIBS). Ted also serves on the executive board of the Long Island Real Estate Group (LIREG) and is a Trustee at Northwell Health's Huntington Hospital. He has worked hand in hand with community officials, specifically in the Town of Huntington, to achieve a Master Plan for the betterment of the Melville Route 110 corridor.
Ted's energies extend beyond TWRC's professional associations as well. He has worked with, and contributed to, a multitude of charities including Toys of Hope, the Make-A-Wish-Foundation, and The Nature Conservancy. He has previously sat on the boards of the Family Service League and North Shore Holiday House.
Ted has been honored by various organizations for his philanthropic work, entrepreneurial spirit, and real estate acumen.
- March of Dimes honored Ted at their 2nd Annual Real Estate Awards Gala in 1999
- Family Service League honored Ted at their 11th annual Great Chefs of Long Island Gala in 2002
- Ernst & Young awarded Ted as a finalist for their Entrepreneur of the Year award in 2002
- The Nature Conservancy – LI Chapter honored Ted at their annual Earth and Ocean Gala in 2006
- Gurwin Healthcare Foundation honored Ted at their Leonard Ugelow Memorial Gurwin Golf Classic in 2008
- The LI Hispanic Chamber of Commerce honored Ted at their 22nd Annual Awards Gala in 2010
- Northwell Health's Huntington Hospital honored Ted at their Annual Gala in 2015
Ted is a graduate of Southern Connecticut State University where he was a four year letter winner in Men's Swimming and earned a Bachelor of Science degree. He is a New York State licensed Real Estate Broker and also a U.S. Coast Guard licensed U.S. Merchant Marine Officer – Passenger Vessel Captain.
Craig Weiss is the President of T. Weiss Realty Corp. ("TWRC"). He oversees and directly negotiates the leasing activity throughout the portfolio, and prepares and analyzes financial models for future T. Weiss ventures. He is also significantly involved in TWRC's operational direction, and is integral in the coordination between the TWRC leasing team and the TWRC construction department.
Prior to joining the family business, Mr. Weiss spent three years in finance - two years in Morgan Stanley's Equity Client Service Group, where he held Series 7 and 63 NASD certifications, and one year as a trader at the hedge fund Scout Capital Management.
Craig is an associate member of the Commercial and Industrial Broker Society of LI (CIBS) and an active member of the Long Island Real Estate Group (LIREG).
He is actively involved with various charitable causes and professional organizations. Specifically, Craig served on The Nature Conservancy's Manhattan based Young Professionals Group's Board of Directors from 2005-2009. Craig was the Co-Chair of their annual gala in 2009. He has also been involved with various fundraising benefits for the Boys Club of New York and the North Shore Land Alliance. He currently serves as a member of the Cold Spring Harbor Laboratory Corporate Advisory Board and, with his father, was the honoree for Northwell Health Huntington Hospital's Annual Gala in 2015.
Craig grew up on Long Island and attended Cold Spring Harbor High School before graduating cum laude from the University of Richmond with a Bachelor of Science degree in Business Administration, concentrating in Finance. Craig is also a member of The Energeia Partnership at Molloy College, Class of 2015 – a regional stewardship program dedicated to identifying and addressing the serious, complex and multi-dimensional issues challenging the Long Island region.
Marci E. Skeahan, Chief Operating Officer at T. Weiss Realty Corp. ("TWRC"), oversees the operations of T. Weiss' office properties on Long Island with responsibility for office operations, the entire company staff, maintenance, facilities, construction and finance. She also serves as the sales and leasing representative for all three T. Weiss companies, and is an expert in lease negotiations and legal proceedings. Mrs. Skeahan has attained a position of prominence and leadership in the traditionally male-dominated field of commercial real estate. Over the past twenty years with TWRC, she has risen through the company ranks, and mentored many women throughout the course of her career. In addition, she plays a leading role in each stage of development as T. Weiss Realty grows and evolves.
Marci Skeahan joined T. Weiss Realty in 1991, and successfully served as Office Manager and later General Manager before being named Vice President of Operations in April 2000. In July 2005, Marci was named Chief Operating Officer. Prior to joining the firm, she managed the Queens office of Charles H. Greenthal, a residential property management firm.
As a board member of the International Facilities Managers Association (IFMA), Mrs. Skeahan has served as Treasurer, and before that, was President for two terms. She served on the Board of Directors of the Long Island Chapter of the Building Owners Management Association (BOMA), the Board of Directors of the Long Island Chapter of CoreNet Global and as member of the Commercial Industrial Brokers Society of Long Island (CIBS).
In December of 2006, Mrs. Skeahan was honored for outstanding accomplishments in business by Women on the Job (WOJ), a not-for-profit advocacy program to advance women in the workplace. She also recently served as Journal Chair for the Nature Conservancy's annual Gala, and has been an active fundraiser for the past nine years for the CoreNet Global golf outing whose proceeds benefit the Make-A-Wish Foundation. In 2003, Marci was honored as one of New York Real Estate's Top 40 Under 40. That same year, Millennium 3 Partners also selected her as a "Long Island Business Diva".
A native Long Islander, Skeahan (neé Glaser) grew up in Setauket and attended the New York Institute of Technology and Hofstra University. She currently lives in Commack with her husband, Cornelius Skeahan. Together they have volunteered with Birch Family Camp, an organization that provides weekly camp programs for inner-city families of those infected with HIV and AIDS.
Lauren Candela is responsible for the firm's marketing initiatives including email campaigns, direct mail, website creation and maintenance, social media, newsletters, advertising, promotional events and public relations.
Prior to joining T. Weiss, Lauren served as a Marketing Assistant at Bed Bath & Beyond where she worked in public relations, social media and in-store promotions.
Lauren currently participates in the Melville Chamber of Commerce, Long Island Real Estate Group, and the Commercial Industrial Brokers Society. She also volunteers as a Wish Granter for the Make-A-Wish Foundation of Metro NY in addition to lending her time to Girls Inc. of Long Island.
Lauren attended Adelphi University where she graduated summa cum laude with a Bachelor of Business Administration degree. While attending Adelphi, Lauren participated as a member of the volleyball team, and earned several athletic and academic honors during her tenure. Lauren also holds a masters degree in Business Administration, with a marketing specialization, from SUNY Stony Brook.
Adam Burke, BBRC's Project Manager, is responsible for the planning, coordination, supervision and control of all projects. With over 25 years of experience in the construction and architectural fields, Adam joined the firm in 2017.
After working in his family's construction business for 15 years, Adam decided to venture into the field of architecture, where he received his degree and began designing luxury homes. Throughout his career, Adam has worked with some of the most influential designers and builders, most recently at a hedge fund managing multi-year, high-profile projects in Manhattan, the Hamptons and across the Northeast.
In his spare time, Adam enjoys spending time with his family.
Meredith Atkinson, T. Weiss Realty's Construction Coordinator, is responsible for the organization, coordination and management of all major construction projects and tenant improvements performed by T. Weiss and BBRC. Prior to joining the firm in 2008, Meredith served as the Service Manager and Logistics Coordinator for On Site Energy Corp. in Hicksville, New York for two years.
Meredith is also a notary public and participates in the MTA's annual toy drive to benefit children in New York.
As a native Long Islander, Meredith attended Kings Park High School and later went on to pursue a Bachelor of Business Administration degree, with a Marketing specialization, at SUNY New Paltz. While attending New Paltz, Meredith was also an active member in an off-campus sorority.
Helene Feldman joined T. Weiss Realty in 2003 as the company's bookkeeper. Eventually, she became the firm's assistant controller and ultimately the controller for T. Weiss. Today, Helene is responsible for all of T. Weiss Realty's accounting functions including accounts payable, accounts receivable and payroll.
Before joining T. Weiss Realty, Helene worked as a Full Charge Bookkeeper for a number of companies in Manhattan including Ann Klein and London Fog.
As a Queens, New York native, Helene attended Queens College and graduated with a BA in Elementary Education and a minor in Mathematics. After getting married, Helene moved to Bethpage, and now lives in Holbrook. She enjoys visiting with her two sons, Jason and Daniel, as well as vacationing on cruises.
Daniella Gonzalez joined our team in 2017 as an Administrative Assistant. Daniella is responsible for general office support, fielding tenant requests as well as assisting the sales team. Prior to joining T. Weiss, Daniella served as Logistics Manager for CCP Solutions in Farmingdale, New York.
Daniella moved to Long Island from Toronto, Ontario, Canada in early 2015 after getting married. Prior to moving, she received a Bachelor's Degree with honors in Kinesiology from McMaster University, a diploma in Massage Therapy and later received a certificate in Business Management from George Brown College.
When she isn't at work, Daniella loves to travel and plays in a women's soccer league on Long Island.


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Ksenia Anske
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"My mama became a catfish when I was two, on the day I stopped talking."
Neglected since birth by her mother, Irina Myshko hasn't spoken a word for most of her short Soviet life. Outcast as a mute idiot and abused by her mother's boyfriends, she escapes into an alternate reality where true natures show and people are revealed as the beasts they are. Pregnant, homeless, and penniless, Irina has to make a choice — learn to live in this splintered world or descend into madness.
Print and signed copies available from the author's website.
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County is first in state to implement faster emergency response technology.
PITTSBURGH, July 21, 2016 – Delaware County, Ohio is the latest municipality to go live with Automated Secure Alarm Protocol (ASAP), a technology that automates communication between alarm monitoring central stations and 911 centers, resulting in improved accuracy and speed of emergency response.
Transmitting data electronically speeds up alarm notification delivery and reduces the number of phone calls and processing time. It also eliminates human error or miscommunication between operators; all pertinent data goes directly to first responders within seconds.
In cities where it is used, ASAP delivers alarm notification information from central stations directly to public safety answering points (PSAPs) via computer rather than by phone, eliminating the need for communication between monitoring center operators and 911 operators.
"We are proud to be the first agency in Ohio to implement ASAP," said Patrick Brandt, Director of Delaware County Emergency Communications. "Our County recognizes the benefits ASAP will bring in terms of better protecting our communities and looks forward to seeing successes early on in this rollout."
ASAP was created in partnership with the Central Station Alarm Association (CSAA) and the Association of Public Safety Communications Officials (APCO), with Vector Security President and CEO Pam Petrow co-chairing the steering committee. Vector Security was the first alarm company to use ASAP in the City of Richmond, VA in 2012. Vector Security helped bring Delaware County online with extensive testing during the conversion.
"There are a number of Vector Security customers in Delaware County who will benefit from this implementation," said Anita Ostrowski, Vice President of Central Station Services, Vector Security. "We hope the positive impact that ASAP will bring to Delaware County will encourage other agencies to adopt ASAP."
"As security provider, we seek ways to promote ASAP's value to municipalities and public agencies, and help other alarm companies implement it for the greater good of the industry and our communities," she added.
"The ASAP implementation in Delaware County follows on the heels of Kernersville and Durham, NC going live with the technology last month," said Bill Hobgood, an ASAP implementation consultant. "We hope the momentum continues as ASAP allows first responders to answer emergencies faster due to improved speed and accuracy of information transmitted."
Other municipalities that have implemented ASAP include Houston, TX; Washington D.C.; James City County, York County and Henrico County, VA; Tempe and Chandler, AZ; Boca Raton, FL; Cary and Guilford County, NC; Kernersville and Durham County, NC; Denton County and Grand Prairie, TX; and Morgan County, AL.
For more information on the benefits and features of ASAP, visit www.vectorsecurity.com/asap.
About Vector Security
For more than 40 years, Vector Security, Inc. (www.vectorsecurity.com) has been a premier provider of intelligent security solutions tailored to the needs of the customer. Headquartered in Pittsburgh, the company offers a full suite of electronic security services for residential, business and national account customers across North America and the Caribbean through a network of branches and authorized dealers. Through its subsidiary, Industry Retail Group (IRG), the company also offers customized managed broadband services that lay the foundation for unsurpassed business intelligence. Vector Security is a sister company of the Philadelphia Contributionship, a mutual insurance company founded in 1752, and currently provides cost-effective, technology-based security solutions to over 300,000 homes and businesses.
Download a pdf of this press release.


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Ballyclare Comrades manager, David Dorrian was in buoyant mood following the surprise win in Bangor.
"What a difference a week makes," he enthused. "Last week's home defeat by Lurgan Celtic cast gloom over Dixon Park and spoiled the weekend for players, fans and officials, but this win has lifted our spirits, and we are now looking forward to our next outing against Donegal Celtic in West Belfast.
"Unfortunately we have no game this weekend, so I hope that the week off won't spoil the momentum gained from the win in Bangor," he cautioned.
Asked about the goal that secured the points, he said that it was a "real cracker". "Adam McCart drilled the ball in to the roof of the net with a volley from the edge of the box, showing technique that you don't expect from a central defender.
"We had as many chances as they had, and William Wharry was unfortunate when his header rebounded off the post, while Jonah Nicholl, who was deputising for Gregg Shannon who has a groin problem, pulled off a great save in the closing minutes to prevent Bangor from salvaging a point."
Commenting on the Chris Trussell – Stuart King partnership up top, he explained: "Stuart played in behind Chris as a number ten, and this worked very well, but after Chris had run himself in to the ground I had to replace him with Randal Reid, who is recovering from a knock picked up against Celtic.
"Eddie Hill also came off the bench, but he developed a hamstring problem, and might be doubtful for our next game against DC."
Asked about the Michael McQuitty injury, he offered: "He had to come off at the start of the second half with a recurring hamstring problem, but he recovers quickly and should be OK (for the next match). He is an important utility player who can play anywhere across the back four and in midfield. Last season he played in central midfield for a Ballymena junior side and scored upwards of thirty goals, so he is a good addition to the squad."
Summing up, Dorrian said: "We definitely deserved to win. We played the better football on the 3G surface, matched them physically, and had the majority of possession, despite them having a lot of experienced players."


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This Karlie Kloss editorial for Interview magazine (simply titled 'Karlie's Couture') features the model in eclectic fashion looks styled by Elin Svahn and photographed by Gregory Harris. The most bizarre of the ensembles is a glittery gem-stone face mask paired with elbow length black gloves, an embroidered bodice gown over jeans and topped off with socks and sandals.
For the tamer looks, Karlie took the skate park, photographed in a glittery black dress and tube socks combo while hanging out with some skateboarders, strutting her stuff in a fire-engine red, parachute-inspired pant, or casually hanging out in a multi-tiered taffeta ball gown. The extravagance of these fashions creates a stunning juxtaposition with the urban environment of the skate park, and Kloss offers a full range of facial expression -- from a unapologetic yawn to an inquisitive stare -- that helps her pull off the eccentric styles.
Offbeat Urban Couture Editorials
More Stats +/-
Exaggerated Urban Editorials
Parisian Tomboy Editorials
Multicolored Urban Editorials
Animated Streetside Captures
Daring Detox Center Editorials
Karlie Kloss's Editorial Features High-End Fashion
- By: Vasiliki MarapasOct 15, 2013
References: thecitizensoffashion & interviewmagazine
References: thecitizensoffashion & interviewmagazine


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We are accepting applications from artists who would like to show their work in our gallery. Contact us!
Hanging June 2 through 30, 2017:
Young Artists' Expo
C4A is pleased to be hosting a show of local emerging young artists' works.
Our show will open with an artists' reception on Friday, June 2 from 6:00 to 9:00 pm, featuring live music as a part of Urbana's First Friday festival.
Young artists showing at this exhibit include:


text: Prelutsky's poem, which originally appeared in The Random House Book of Poetry for Children (1983), propels French artist Davenier's cheerful watercolors. The poem's quartet of four-line stanzas riffs on the theme of personal uniqueness: "There is no other ME I AM / who thinks the thoughts I do; / the world contains one ME I AM, / there is no room for two." The poem repeats three times, with Davenier successively supplying three active children whose rooms brim with the trappings of their intense interests. A girl who loves raucous pretend play—abetted by a cape, pirate wear and a goldfish sidekick—leads off. A boy who loves nature and tending its creatures is next, followed by a brown-skinned girly girl who dances before a row of stuffed toys. The artist uses appealing spot illustrations to reveal each child's disparate imagination at work. On the last few spreads, the trio converges, joining a phalanx of milling children celebrating both the concept, and the giant, yellow-lettered word: "ME!" Slight, but not without its charms. (Picture book/poetry. 3-6)


text: Hard to believe commencement is in less than a month. That means these are the final few shows, including this week's episode, with two guests. One was NBA veteran Scot Pollard, which included a question on his famous "hey kids, do drugs" moment. The other was Rich Grisham of OOTP Developments, who discussed the company's recent releases: Out of the Park Baseball 18 and MLB Manager 2017. Listen below and subscribe to Big Red Sports Talk podcasts on iTunes, Stitcher, and Google Play!


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Ms. Patricia Nickler - Principal
Mrs. Tina Haky - Administrative Assistant
Mrs. Alison Testa - Guidance Counselor
Mrs. Tiffany Bell-Zylka - Business Manager
Mr. Alan J Dines, Athletic Director, Social Studies and P.E.
Mr. Martin Pierce - Head of Maintenance
Advisory Council
Mike Delmar
J.D. Ewing
Father Willie Lechnar
Lori Omatick
Ed Plisko
Mary Jane Speeney
Mr. Nick Bell - Music and Performing Arts Department
Mr. Sal Brusco - Math Department
Mrs. Jamie Brooks - Math Department and P.E.
Mrs. Marian Cadwallader - French Department
Mr. Chris Dangelo - Social Studies Department
Sra. Sandra Kantor - Spanish Department
Mr. Jeffrey Schrecengost - Science Department


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Home > Continuing Legal Education > Audio/Video Products > Writing the Winning Brief Tips from the Bench and Bar
Product Code: 2285
Presented on: Tuesday, June 27, 2017
1.5 CLE credits: Skills
Writing the Winning Brief is presented by:
Hon. Lawrence J. Vilardo, U.S. District Court, WDNY
Hon. Victoria A. Graffeo, Harris Beach PLLC, Ret. Judge, N.Y. Court of Appeals
Hon. Eugene F. Pigott, Jr., NYS Supreme Court, Ret. Judge, N.Y. Court of Appeals
Erin M. McCampbell, Esq., Lipsitz, Green, Scime Cambria LLP
Timothy P. Murphy, Esq., Chair, Appellate Practice Committee
Available on CD, DVD or OnDemand
CD/DVD: $40 Member / $50 Non-Member
Shipping: $5
ON DEMAND: $45 Member (PROMO CODE: member2017), $60 Non-Member
Make Selection Below to Order CD/DVD Online:
Please indicate shipping or pick-up:


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AEA hopes that each session is welcoming, inclusive, fair, respectful and most importantly, an opportunity for professional learning and networking. Towards that end, the AEA Conference Policy Committee has developed the following guidelines for serving as a chair, discussant, or presenter at Evaluation 2007:
Guidelines for serving as a Session Chair
The quality and tenor of the session depends greatly on the active and early participation of the session chair. The chair can build a network among the presenters, set the tone for the session, make connections among the people and topics, ensure fair allocation of the time available, and foster collegial and professional exchange. Should challenges arise, the chair will be looked to for guidance. Throughout the session, the chair will be looked to for leadership. The role of the chair ideally includes both pre-conference preparation and during-conference facilitation. We offer these guidelines to you as the chair of a valuable conference session.
Pre-conference: At least one month before the conference, please connect with all of your presenters, including discussants, co-chairs, etc., as a group, in order that each may learn from the other and the session may go as smoothly as possible. Once the program is posted, in July, you will be able to see the list of everyone in your session, with their email addresses, by going to the online program. Pre-conference, as a chair you should:
Use technology to connect: Begin by emailing everyone as a group, then consider setting up a blog or connecting via phone. Example letter
Help to build professional networks: Ask each presenter to introduce him or herself and the focus of their presentation. Encourage discourse.
Set the ground rules for the session: Lay out the format for the session, and discuss as needed, noting the time to be allocated for each portion. If this is a paper session, each paper will be limited to fifteen minutes, including questions, until everyone has had an opportunity to present. If there is a discussant (or discussants) work with them to identify the length of their contribution. Be sure to reserve time for audience questions at the end.
Exchange materials electronically: Ask that the presenters share any materials available in advance of the session including slides, papers, or resource links. Encourage presenters to build upon one another's work and identify linkages in their presentations.
At the session: The time has come - seize the opportunity to build a learning community in your session room! At the session, as a chair you should:
Arrive early and greet those in attendance: Locate and welcome your speakers and attendees and make everyone feel at ease.
Start on time and end on time: Maximize the time allocated. Encourage people to sit and focus as the session start time approaches. Be courteous to the next group and allow time for transition between obligations by ending on time. Get the presenters in place as early as possible.
Introduce each presentation: Transition between presentations by thanking the previous presenter and introducing the next, noting the name and affiliation of the individual or group and the general topic. Example transition language
Facilitate discussion: During discussion periods, keep notes as to who has raised hands and call on people in turn. Set an expectation for professional, courteous, discourse. Ask that questions be short and targeted and encourage attendees to follow-up after the session as well. If there are many people with questions, avoid letting one person monopolize the discussion.
Repeat back questions from the audience: to be sure that everyone heard the question clearly. The audience member is likely facing front and those behind her or him may find it difficult to hear the question posed.
Wrap-up and end: Provide a clear conclusion to the session by thanking the presenters and the audience and stating that the session has concluded. At the end of the session, if there is a session following, ask that everyone leave the room and continue the discussion in the foyers so that the next session will have time to set up.
Troubleshoot: As the chair, people will turn to you should problems arise and expect you to manage the session and respond to challenges.
Setting and room: Should the overhead projector not work, the room be too
hot or too cold, or something happen to the room itself (a spill, noise
intrusion, etc.), contact the hotel or the AEA staff using either an
in-house phone or by sending someone to the registration desk (do not go
Long-winded presenters: Should someone go over the allotted time, and not
heed your cards noting that time is up, you may try initiating applause
during the next pause, or simply walk to the front and thank the presenter
for the presentation but note that the session must move on to the next
presenter. This will be far easier if you have laid the ground rules in
advance of the session via email and at the start of the session in your
introduction. Example language
Pre-conference - example letter: "Evaluation 2008 Colleagues, I am the chair for the multipaper session entitled 'Surveys as a Data Collection Tool.' This year, each chair has been asked to connect with the presenters in his or her session in advance of the conference so that we may know of each other's work, improve the flow of the session, and set some timing guidelines. I work at the University of West Florida's Data Center and am looking forward to meeting you all in person. Would you each be kind enough to a) introduce yourself and your topic, and b) share any materials - notes, slides, papers, links - as they become available? I also want to take this opportunity to set the basic ground rules for our session (and for all multipaper sessions). We'll open with a very brief introduction from me to be followed by each of you in turn, in the order that you appear in the conference program. Each presentation is allocated fifteen minutes during which you are welcome to take questions, or not, at your discretion. However, after the fifteen minutes have elapsed, we will need to move on to the next presentation in order to be fair to everyone. After the presentations, I will facilitate a question-and-answer period from the audience. To help keep us all on track, we'll be using timing cards to warn you when you have three minutes left, one minute left, and when it is time to stop. Please 'reply to all' so that we may all learn, one form the other. I look forward to our exchange and to seeing you in Denver!" Return
Set the stage - example language: "Welcome everyone to multipaper session 307 focusing on using surveys as a data collection tool. Thank you for joining us on this lovely Friday morning. My name is Shara Ibaru. I work at the Data Center at the University of West Florida, and I will be serving as the session chair. We have ninety minutes for today's session and four presentations. Each presentation will be allocated 15 minutes during which time the presenters may take questions at their discretion. However, once the time elapses for each presentation, we will move on to the next and ask that further questions be held until after all of the presenters have had the opportunity to present. I will stop presenters who run over their allocated time out of fairness to all. Today we'll be hearing about innovations in surveying using new technologies, useful ways to access difficult to survey populations, and how a targeted survey provided actionable information to a multifaceted nonprofit. Together, today's presentations offer both process and product lessons for improving our own surveying practices. Let's begin." Return
Introduce each presentation - example transition language: "Cori and Anu, thank you for sharing your work. Now we will turn to our colleagues from Think and Do Consulting so that we may learn about the strategies they used to access and survey homeless men and women in Detroit. Welcome!" Return
Long-winded presenters - example language: "Manuel, thank you for your time.
I realize that you have more to share, but we must move on to be fair to all
of the presenters. I encourage everyone to reconnect after the session, or
after the conference via email, to learn more."
Guidelines for serving as a Discussant
While not all sessions have discussants, when one is present, that person serves a pivotal role in tying the multiple components of the session together. Discussants are expected to respond from their own base of knowledge rather than to conduct further research on a topic. However, discussants should participate in pre-conference discussions and exchange of information, and be prepared and thoughtful in their answers. As a discussant, you should strive to foster the exchange and development of ideas and the professional growth of the presenters and the audience.
Pre-conference: We are asking all session chairs to connect with the presenters in their sessions, including any discussants, as a group via email, before the conference. Pre-conference, as a discussant you should:
Participate in pre-conference discussion: The pre-conference exchange will give you the opportunity to learn about the session content and prepare remarks.
Review materials: We are encouraging, but not requiring, the exchange of materials (notes, slides, resources, papers) among presenters pre-conference. Take the time to review the materials to familiarize yourself with the session content.
Be a mentor: Often, discussants are more senior or more experienced. Provide guidance, where appropriate, regarding questions that might be raised by attendees, resources that might strengthen a presentation, and what to expect at the conference and session.
Air concerns: If you have concerns about a presentation (methodology, theoretical base, etc.), share them with the presenter before the conference. Be a critical friend, striving to coach and support colleagues. Encourage the presenter to have a strong response to any concerns that you intend to share at the session.
Prepare notes: Looking across the presentations, try to find the commonalities that weave them together.
At the session: This is your time to listen intently to each presentation, take notes, and provide your thoughtful response. At the session, as a discussant you should:
Arrive early: Arrive at the session early and connect with the other presenters and session chair so that the session may start on time.
Respond thoughtfully: Especially in paper presentations, you are the linchpin that ties together the many, sometimes disparate, presentations. A few dos and don'ts
Succinctly identify common themes among the presentations
Tie the presentations to a broader content
Critique rather than deride, taking on the role of a 'critical friend'
Emphasize application and ways to learn more about the topic
Restate what each presenter said - the presenter's have already done that
Walk through each presentation in turn with a detailed response
Give an additional presentation on the topic - you are responding to what you heard
Stay on time: Stick to the time agreed upon during the pre-conference
exchange in order to be respectful of the presenter's and audience's desire
to interact.
Guidelines for serving as a Presenter
Presenters share their knowledge and expertise through the 500+ presentations at the event. As such, you are the backbone of the conference. Presenters need to prepare in advance, deliver content articulately and concisely, and follow-up to build knowledge networks. Please note that the guidelines below have intentionally be left broad so that they apply to all session types with the exception of posters and roundtables which are unique among AEA offerings. If you are presenting a poster, click here for poster guidelines, and if you are presenting a roundtable, click here for roundtable guidelines.
Pre-conference: We are asking all session chairs to connect with the presenters in their sessions, as a group via email, before the conference. Pre-conference, as a presenter you should:
Participate in pre-conference discussion: The pre-conference exchange will give you the opportunity to learn about colleague's presentations as well as the session's timing and pace. It will allow you to coordinate with others in your session to limit overlap and respond to one-another's work.
Share materials: We are encouraging, but not requiring, the exchange of materials (notes, slides, resources, papers) among presenters, chairs, and discussants, pre-conference. Share your materials as part of the pre-conference email exchange.
Prepare handouts: At a minimum, you should have 50-100 copies, depending on room size, of an abstract for your presentation that includes your name and contact information, including email, for follow-up to learn more and access longer documents, including full papers for paper presentations.
Prepare your presentation: Consider the time available, the audience's likely desire to ask questions, and the multiple learning styles of attendees (auditory, visual, etc.), when preparing to create a memorable and valuable presentation.
Identify the time allocated: As part of the ongoing pre-conference discussion, clarify the time to be allocated to your presentation. If you are presenting a paper, you will have 15 minutes. However, if you are part of a panel, demonstration, think tank, etc., then you will need to determine among the presenters how much time is to be devoted to what content.
Determine when questions and discussion will take place: Again, as part of the pre-conference discussion, identify the time to be devoted to questions and discussion and whether you will take questions during your presentation or only afterwards. If you are presenting a paper, you may choose to take questions during your 15 minute presentation time; however, you must cede the floor at the end of 15 minutes, and there will be an open question time at the end of the session.
Plan your presentation: Create an outline for yourself of the key points to be conveyed and then develop notes regarding what you wish to share relating to each key point. Develop visual aids (see below) to illustrate your key points and serve as an outline to the session.
Prepare visual aids: The vast majority of presenters use
overhead transparencies, PowerPoint slides, or other visual aides as part of their presentation. Each room is equipped
with a traditional transparency projector for plastic transparencies, and
LCD projector, a computer and a screen.
Type: Use at least 24 point type so that it may easily be read from across the room. Avoid italics and ALL CAPS for more than a few words as they are difficult to read.
Bullets: Limit yourself to at most 6 bullets per slide and 10 or so words per bullet. Describe details verbally and use the bullet points to provide an outline of key concepts.
Number: A rough rule of thumb is to prepare no more than one slide for every two minutes you will be presenting. This is an upper-limit. The slides are an aide, not the presentation itself.
Avoid acronyms, jargon, and abbreviations: Past evaluations have clearly indicated that one frustration, in particular for new and international attendees, is the use of 'insider' language, acronyms, and abbreviations that make it difficult to comprehend readily a presentation.
Contact information slide: Prepare one slide that you can put up at the beginning and end of the presentation with your presentation title, name, and contact information. In case you do not have enough handouts, encourage attendees to write down this information for follow-up.
Proofread and spell-check. Spell-check and proofread. Please.
Practice: Practice repeatedly, alone and then in front of a colleague, to ensure that your presentation highlights key point, your delivery is clear, and you can finish within the time allocated.
At the session: This is your time to listen intently to other's presentations and to share your own knowledge and expertise. At the session, as a presenter you should:
Arrive early: Arrive at the session early and connect with the other presenters and session chair so that the session may start on time.
Identify who will be holding the timing cards: Timing cards in each room identify "3 minutes", "1 minute" and "Stop" to prompt presenters. If these will be used for your session, identify who will be holding them so that you may watch them during your presentation.
Give your presentation: You have already practiced and prepared - you are ready! Speak clearly, maintain eye contact with the audience, and relax. Stick to the pre-agreed upon time for your portion of the session to ensure that everyone has the opportunity to present and interact with the audience.
Respond to questions: Whether you take questions during your presentation or at the end, you should be prepared to respond to inquiries. Be aware of the limited time in the session and offer concise responses, noting - when appropriate - that you may be able to follow-up post-session or post-conference to continue the conversation.
Depart on time: At the end of the session, if there is a session following, leave the room and continue the discussion in the foyers so that the next session will have time to set up.
Post-conference: After the event is over, you likely will receive emails or
calls from those who heard, or heard of, your presentation. If you were
making a paper presentation, you should be prepared to email the completed
paper. This is an opportunity to build your professional network and sustain
the field through collegial exchange. Where appropriate, you may want to ask
those inquiring about their work to see how it might mesh with your own in
ways that could be advantageous to you both.


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|Swayback is a Scrabble word. Scrabble point value for swayback: 22 points.
|Swayback is a Words with Friends word. Words with Friends point value for swayback: 22 points.
Above are the results of unscrambling swayback. Using the word generator and word unscrambler for the letters S W A Y B A C K, we unscrambled the letters to create a list of all the words found in Scrabble, Words with Friends, and Text Twist. We found a total of 71 words by unscrambling the letters in swayback. Click these words to find out how many points they are worth, their definitions, and all the other words that can be made by unscrambling the letters from these words. If one or more words can be unscrambled with all the letters entered plus one new letter, then they will also be displayed.
1. having abnormal sagging of the spine (especially in horses)
|Words that start with swayback
|Words that end with swayback
|Words that contain swayback


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Specialty Hotel Concierge Helps Guests Discover The Paris Art Scene
Visitors to this luxury hotel can now ask an expert on art-related topics.
Le Royal Monceau is a luxury hotel situated in Paris, France. Recently, the hotel hired an art expert to help guests learn more about Parisian art. Domoina de Brantes is the Art Concierge and can answer any questions guests might have relating to art, design, and culture. Guest can ask an array of questions, including purchasing art, opening dates of new exhibitions, and even where to spot good street art.
Brantes will also give complementary guided tours upon requests where guests can enjoy a stroll around the city and discover its art scene. Brantes' private art tours are becoming highly popular, so interested guests need to book in advance.


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While regular carpet cleaning by professionals is necessary to ensure your carpet leads a long and healthy life, basic maintenance on your part can help maintain cleanliness and duration between major services. Proper care and maintenance of your carpet will extend its useful life and preserve its appearance longer. Failure to maintain proper care will result in premature wear. Additionally, clean carpets contribute to a sanitary, healthy, and fresh scented indoor environment.
- Vacuum your carpets three times a week using a vacuum cleaner with a HEPA (high-efficiency particulate) filter.
- Remove stains and spills as promptly as possible – This is best achieved with Best Coast Carpet Solutions Professional Strength Spot Remover. Remove as much excess as possible, then apply and blot with a clean white cloth. DO NOT rub the spot as this might grind the stain in further.
- Have your carpets cleaned and protected at least twice a year by Best Coast Carpet Solutions, or more often depending on soil level from traffic, children, or pets.
Best Coast Carpet Solutions also offers useful carpet care tips and hardwood care tips.


text:
China Pulls The Rug From Under Europe, Halts French Bank Transactions, Makes Good On Trade War Ultimatum
by Tyler Durden
A flurry of headlines out of China suggest global macro-economic volatility may be ready to take it to the next level. We discussed last week how China's oh-so-generous offer of help to Europe was merely a veiled threat playing US against Europe in a game of who-gets-the-funding. Well, tonight, it seems, they are making good on some of those threats. Aggravated by EU's lack of market economy recognition, they pull trading lines with French banks, express concern at the EUR's safety (preferring US Treasuries), and indicate a clear preference for bonds over stocks – all the while warning of growing trade tensions – consider the sabre-rattled.
Initial comments from Commerce Minister Shen via Bloomberg:
The Rest…HERE


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They may not be the flashiest aspect of your bathroom, but there's no denying that toilet seats are absolute essentials. In fact, your toilet seat can really tie the room together thematically. That's why at The Whirlpool Bath Shop we offer a wide range of toilet seats with a wide range of designs. Whether you want something rustic with a delightful wood finish, or you're after a classic white gloss look for your toilet seats, you'll find it here. Manufactured by brands like Moods, these toilet seats aren't just practical - they're easy on the eye too. You'll even find high-tech toilet seats with LED lights and the sought-after soft-close toilet seats. Have a question? Just ask!
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Just as with any other small aspect of your bathroom, toilet seats require considerable consideration before you make a choice. Do you go modern or traditional? Contemporary or wood grain? We're sure you have plenty of questions bout which toilet seats are the right ones for you. If so, please do get in touch with our friendly team at The Whirlpool Bath Shop. We'd be more than happy to talk you through installation of your toilet seats and much more.


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I can't believe Friday's here already. This week seems to have gone in a flash. Things have been pretty busy, which is good but also requires a certain knack for controlling the tendency to get stressed when attempting to do several things at once. That can be tricky at the best of times but I came across this on Miss Moss's blog and thought it would be good to share it since it contains some wise words:
It also reminded me of this song, which always makes me feel better and puts things into perspective:
Most of you will probably be familiar with it since it was a worldwide hit in 1999 but no harm in listening to it again, I say. Interestingly, I just found out that it was inspired by "Advice, like youth, probably just wasted on the young", an article written by Mary Schmich published in the Chicago Tribune in 1997. That's a fact kids - you learn something new with me every day ;).
I hope you all have a lovely weekend and make sure to relax/dance/eat cake/sleep well/get lots of fresh air and sunshine (delete as appropriate) and of course take plenty deep breaths should you encounter any obstacles to fun times!


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FENDINA is a trend jewellery chief that equip with a set of unique design, high-quality manufacturing and gross sales. We deal with the distinctive model of jewellery design, strictly management the standard of merchandise and maintaining with trend tendencies from 1993. Being the main target with the FENDINA within the marriage ceremony,engagement,cocktail,commencement, promenade or different events, FENDINA trend jewellery, present you a superb items concept on Valentine's Day, Mother's Day, Christmas or jewellery items for her. Exquisite look with the can¡¯t beat worth, select FENDINA engagement rings marriage ceremony band, classic crystal bracelets,CZ earrings fairly pendant necklace and jewellery units. FENDINA, More than you count on!AAA+ Gorgeous Cubic Zirconia
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Amazon and the Amazon brand are emblems of Amazon.com, Inc. or its associates.


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Our client is a well established and respected international freight forwarding and logistics solution provider with offices in in major ports around the country. . They are in the process of recruiting an experienced Accounting professional to join their finance team based at their head office in Kelana Jaya, PJ.
The opening position is full time permanent position with the objective to manage efficient accounting functions within the warehousing activities. In this regard, Chrisjac working closely with the management of our client to search for the right candidate who is qualified for the role.
Salary for the successful candidate will between RM 3500-4500.
About the job
This is a permanent senior executive level position where the incumbent will report to the Accounts Manager of the group. The successful candidate will be responsible for managing full spectrum of accounting functions independently. You will focus on managing month end activity accounts preparation of the warehousing activities, inter-company invoicing and consolidation.
The candidate must have exposed to full end-to-end cycle of the payable and accounts receivable and full set of accounts. You will be involved in month end responsibilities including accruals, prepayments and reconciliation invoices, balance sheet reconciliation, statutory reporting and GST returns.
The candidate
We are looking for individuals with at least 5 years of relevant experience to undertake this role. We are looking for female candidate only with knowledge and ability to manage and prepare full set of accounts.
Candidate who will succeed to take up this role must possess minimum Diploma qualification in Accountancy or at best Bachelor degree graduate in Accounting or ACCA/CIMA graduate. Candidates who aspire to hold this position must be proficient in English plus conversant in BM and Chinese/Mandarin. In addition, candidates who wish to apply must ensure she will be able to work in Kelana Jaya area.
To apply online, send your resumé to firstname.lastname@example.com, or click the "Apply Now" button below.


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- Review
- Open Access
Zinc finger proteins in cancer progression
- Jayu Jen1 and
- Yi-Ching Wang1, 2Email author
© The Author(s). 2016
- Received: 12 May 2016
- Accepted: 1 July 2016
- Published: 13 July 2016
Zinc finger proteins are the largest transcription factor family in human genome. The diverse combinations and functions of zinc finger motifs make zinc finger proteins versatile in biological processes, including development, differentiation, metabolism and autophagy. Over the last few decades, increasing evidence reveals the potential roles of zinc finger proteins in cancer progression. However, the underlying mechanisms of zinc finger proteins in cancer progression vary in different cancer types and even in the same cancer type under different types of stress. Here, we discuss general mechanisms of zinc finger proteins in transcription regulation and summarize recent studies on zinc finger proteins in cancer progression. In this review, we also emphasize the importance of further investigations in elucidating the underlying mechanisms of zinc finger proteins in cancer progression.
- Zinc finger protein
- Transcription factor
- Cancer progression
Transcription factors play a central role in regulating gene expression, and therefore coordinate a plethora of biological processes, including differentiation, development, metabolism, apoptosis, autophagy and stemness maintenance [1–5]. Based on different DNA binding motifs, transcription factors can be majorly categorized into classical zinc fingers [6], homeodomains [7], and basic helix-loop-helix [8]. Among these, classical zinc finger containing proteins (ZNFs) form the largest family of sequence-specific DNA binding protein, which are encoded by 2 % of human genes [9, 10]. To date, 8 different classes of zinc finger motifs have been reported, including Cys2His2 (C2H2) like, Gag knuckle, Treble clef, Zinc ribbon, Zn2/Cys6, TAZ2 domain like, Zinc binding loops and Metallothionein [11]. Different types of zinc finger motifs show great diversity of biological functions. Notably, in addition to DNA binding, studies have recently revealed the RNA, protein and lipids interacting abilities of zinc finger motifs [12–15]. Therefore, with different combinations of multiple zinc finger motifs, ZNFs can greatly expand their diverse role in gene regulations under different cell contexts or stimuli. The general mechanism of gene regulation by ZNFs and their great variety of roles in cancer progression will be discussed in this review.
The transcription regulation of ZNFs
C2H2-type zinc finger motif is the largest group of all zinc finger motif classes. According to the InterPro database (updated on April 14th 2016), there are 5,926 members in the C2H2-type ZNF family. C2H2-type zinc finger motif is composed of CX2CX3FX5LX2HX3H, and its two cysteine and two histidine residues fold into a finger-like structure of a two-stranded antiparallel β-sheet and an α-helix after interacting with zinc ions [16, 17]. Studies have demonstrated that two to three successive C2H2-type zinc finger motifs are the most suitable unit for DNA binding [6]. In addition, GC-rich or GT-rich sequences serve as C2H2-type ZNF cis-regulatory elements. For example, CTGGCAGCGC has been revealed as SP1 consensus binding element to transcriptionally activate BRK1 expression, while (T/A)(G/A)CAGAA(T/G/C) is the consensus element for ZNF217 to suppress E-cadherin expression [18, 19].
In addition to tandem zinc finger motifs, C2H2-type ZNF also contains other functional domains, such as BTB (Broad-Complex, Tramtrack, and Bric-a-brac)/POZ (poxvirus and zinc finger), the Krüppel-associated box (KRAB), and SCAN (SRE-ZBP, CTfin51, AW-1 and Number 18 cDNA) domain. These functional domains may control subcellular localization, DNA binding and gene expression by regulating selective binding of the transcription factors with each other or with other cellular component. For instance, zinc finger protein GATA-1 has been reported to interact with different partners, including Fli-1, Sp1, EKLF and PU.1 [20–22].
ZNF proteins can carry out different functions with different partners and even elicit opposing actions on different partners. For example, physical interaction between GATA-1 and Fli-1, a member of Ets family of transcriptional activator, cooperatively activate the expression of megakaryocyte-specific genes, including GPIX and GPIbalpha, at transcriptional level [20]. In contrast, interacting with PU.1, another Ets family member, blocks GATA-1 DNA binding ability and therefore inhibits erythroid differentiation [23]. Recent study also shows that ZEB1, a transcription repressor of differentiation-associated genes, turns its function into a transcriptional co-activator of a common ZEB1/YAP target genes through interacting with YAP and therefore leading to aggressive cancer phenotype [24].
Studies have demonstrated that ZNF proteins show diverse regulation mechanisms on a wide variety of downstream genes through recruiting different chromatin modifiers. Some ZNF proteins work as transcriptional repressors by recruiting co-repressors [25–27]. For example, ZNF217 has been found to suppress downstream gene expression by interacting with co-repressors, including CoREST, lysine demethylase 1, histone deacetylase 2 and C-terminal binding protein [25]. Some ZNF proteins, on the other hand, work as transcriptional activators by interacting with co-activators, including CBP/p300 and C/EBP [28, 29]. These studies clearly indicate that trans-acting proteins play important roles in determining ZNFs as transcription activators or repressors.
Post-translational modifications on ZNFs
The post-translational modifications (PTMs) of ZNFs, especially acetylation and phosphorylation, add another layer of regulation for ZNFs in which transcription may be activated or repressed. GATA1, a transcription factor that contains 2 highly conserved zinc finger motifs, is found acetylated at the lysine residues adjacent to the C terminal zinc finger by acetyltransferase CBP and p300. Acetylation of GATA1 shows stable association with chromatin probably by facilitating protein interactions, such as bromodomain-containing protein Brd3 [30–32]. Erythroid Krüppel-like factor, also known as EKLF, is acetylated at lysine residues 288 and 302 near its zinc finger motif mediated by CBP and p300 [33]. The acetylated EKLF at lysine residue 288 can transactivate β-globin expression through recruiting the large erythroid complex (ERC-1) that contains SWI/SNF chromatin-remodeling proteins and histone 3.3 [33, 34]. Another C2H2 zinc finger protein, YY1, is acetylated by p300/CBP associated factor (PCAF) at its zinc finger motif and inhibits its DNA binding capacity. Acetylation mediated by p300 and PCAF at the central glycine-lysine rich domain of YY1, however, does not affect DNA binding affinity but fully suppresses target gene transcription [35].
Phosphorylation on serine or threonine residues of the ZNFs linker peptide has been reported [36]. ZNFs, including Ikaros, Sp1 and YY1, are found to be highly phosphorylated on threonine/serine residues of their linker peptide during mitosis and therefore abrogated their DNA binding ability [37, 38]. Rizkallah et al. generated an antibody raised against phosphorylated linker peptide TGEKP to show that about 50 % of all linkers in 80 % of C2H2-type ZNFs are phosphorylated, indicating that phosphorylation is a highly coordinated mechanism to keep ZNFs away from DNA during mitosis [39].
The oncogenic ZNFs in cancer progression
Summary of differential roles of ZNF proteins in cancer progression
Cancer models
Target genes
Mechanism in tumorigenesis
Colorectal cancer
Integrin β4 ↑, VEGF ↑
Promotes cancer cell growth, migration, angiogenesis, proteolysis
Multiple myeloma
Cyclin D2 ↑
Enhances cell proliferation
Prostate cancer
Promotes cell migration
Cervical, colon, ovarian cancer, neuroblastoma
Suppresses autophagy and lysosome biogenesis
Lung cancer
Chromosome locus 6p22.1 is amplified
ADD1 ↑, CCND1 ↑, p53 ↓
Promotes cell growth, migration and invasion
p14ARF ↓, p15INK4B ↓, p16INK4A ↓
Suppresses tumor suppressor genes through recruiting a co-repressor complex, including DNMT1
Ovarian cancer
Integrin β1 ↑
Activates Src/focal adhesion kinase and paxillin and therefore prevents anoikis
Gastric cancer
Serves an independent prognostic factor for gastric cancer patients
Survivin ↑, x-IAP ↑, Bcl2 ↑, Caspase-3 ↓, Bax ↓
Promotes cell proliferation and inhibits apoptosis
Promotes cell migration and invasion
MDR-1/P-gp ↑, MRP1 ↑, Bcl-2 ↑, Bax ↓
Contributes to multi-drug resistance
Hepatocellular carcinoma
Nanog ↑, SOX2 ↑
Confers self-renewal properties and chemoresistance
Nasopharyngeal carcinoma
E-cadherin ↓
May be involved in EMT
Glioma, lung, oral, breast cancer
Promotes cell proliferation and survival
Gastric cancer
Promotes cell growth through up-regulating ERK-MAPK pathway
Gallbladder cancer
Promotes proliferation, migration and invasion potentially through activation of PI3K/AKT pathway
c-Myc ↑
Promotes glioma stem cell maintenance
Breast cancer
Promotes TGF-β-induced EMT
SHP-2 up-regulates ZEB1 expression to mediate EMT, invasion and growth
Cervix, breast cancer, osteosarcoma, adrenal carcinoma
E-cadherin ↓
Down-regulates E-cadherin and cell polarity factors by recruiting co-repressor CtBP or BRG1
Cervix, colorectal cancer
Activates genes involved in TGF-β/BMP signaling by recruiting p300 and P/CAF
Lung cancer
E-cadhein ↑, ST14 ↑, Vimentin ↑
Confers EMT-related acquired resistance to EGFR-TKI
Breast cancer
Promotes angiogenesis
Nasopharyngeal, esophageal, lung, gastric, colon, breast cancer
Induces cell apoptosis by repressing ribosome biogenesis and NF-kB and AP-1 signaling
Gastric cancer
Inhibits cell growth, migration and invasion
Gastrointestinal, esophageal cancer
Promoter hypermethylation is found in various cancer types
ZNF191, Kox17
Breast cancer
Inhibits angiogenesis
Gastric cancer
miR940 promotes cancer migration and invasion by targeting ZNF24
Breast cancer
Suppresses cell proliferation by promoting MDM2 autoubiquitination and therefore p53 stabilization
Involved in DNA repair by regulating chromatin relaxation and recruiting repair proteins to DNA lesions
Gastric cancer
miR-199a-3p promotes cell proliferation and suppresses apoptosis by targeting to ZHX1
CCND1 ↓, CCNE ↓, Bcl2 ↓, Bax ↑, cleaved Caspase-3 ↑
Induce G1/S arrest and apoptosis
Ewing's sarcoma, osteosarcoma, renal cell carcinoma
Overexpressed in various cancers
Induced under hypoxia stress
Skin and cervix cancer, glioblastoma
Supports inflammation and cancer progression
Liver cancer
miR-525-3p promotes cell migration and invasion by targeting ZNF395
Breast and colon cancer
Suppresses cell proliferation
Breast cancer
Vimentin ↑, Slug ↑, ZEB1 ↑
Involved in TGF-β-mediated metastasis
Prostate cancer
miR-31 ↓
Promotes cell migration and invasion
Breast and colorectal cancer
HIF-1α ↓
ZNF322A, also known as ZNF388 or ZNF489, consists of 11 tandem repeats of C2H2 zinc finger motif. ZNF322A was first identified as oncogene by Lo et al., showing that ZNF322A residing region is amplified in both Asian and Caucasian lung cancer patients [44]. Further study reveals that ZNF322A promotes cell proliferation, migration and invasion through transcriptionally activating cyclin D1 and alpha-adducin but suppressing p53 in lung cancers [45]. Multivariate Cox regression analysis indicates ZNF322A is an independent risk factor of poor outcome in lung cancer patients [45]. Notably, ZNF322A mouse ortholog, Zfp322a, is reported as a novel essential component of the transcription network, which maintains the self-renewal and pluripotency of mouse embryonic stem (mES) cells [46]. Zfp322a promotes OKSM (Oct4, Klf4, Sox2, c-Myc)-induced mouse embryonic fibroblast reprogramming to mES cells by transcriptionally activating Oct4 and Nanog expression [46]. The study on Zfp322a implies a potential role of human ZNF322A in maintaining the pluripotency of embryonic stem cells or cancer stem cells.
ZNF304, which contains a KRAB domain and 13 C2H2 zinc finger motifs, was first identified by AU-motif directed display and RACE in 2002 [47]. ZNF304 plays a pivotal role in silencing tumor suppressors, including p14ARF, p15INK4B and p16INK4A, through recruiting a co-repressor complex that includes DNA methyltransferase DNMT1 [48]. In addition, an integrative bioinformatic analysis of The Cancer Genome Atlas ovarian cancer dataset and experimental validation reveals the association between ZNF304 and ovarian cancer metastasis [49]. The authors show that ZNF304 transcriptionally activates integrin β1 expression, which subsequently activates Src/focal adhesion kinase and paxillin and eventually prevents anoikis [49]. Using delivery of ZNF304 siRNA by a dual assembly nanoparticle, these authors successfully conducted a sustained ZNF304 silencing which increased anoikis and reduced ovarian tumor growth in orthotopic mouse models [49].
ZNF139 is significantly overexpressed in gastric cancer patients. Cox survival analysis reveals ZNF139 overexpression as an independent prognostic factor for gastric cancer patients [50]. ZNF139 has been reported to promote proliferation and inhibit apoptosis through up-regulating the expression of Survivin, x-IAP and Bcl-2, and down-regulating Caspase-3 and Bax [51]. In addition, ZNF139 promotes cancer migration and invasion in gastric cancer by increasing the expression of MMP-2, MMP-9 and ICAM-1, and decreasing the expression of TIMP-1 [52]. ZNF139 also contributes to multi-drug resistance by enhancing the expression of MDR-1/P-gp, MRP1, Bcl-2 while inhibiting Bax expression [53].
Overexpression of zinc finger protein, X-linked (ZFX) has been shown to promote cell growth and metastasis in laryngeal squamous cell carcinoma, glioma, non-small cell lung cancer, gastric cancer, oral squamous cell carcinoma, gallbladder cancer and breast cancer [5, 54–60]. In addition, ZFX is found to confer self-renewal properties and chemoresistance in hepatocellular carcinoma through transcriptional activation of Nanog and SOX2 expression [5]. Fang et al. also showed that ZFX transcriptionally up-regulates c-Myc expression leading to glioma stem cell maintenance [61]. Inhibition of ZFX using siRNA oligo or drug treatment suppresses cancer progression, indicating the potential of oncogenic ZNFs as therapeutic targets [62, 63].
Zinc finger E-box-binding homeobox, ZEB1, is a well-studied transcription factor involved in Epithelial-Mesenchymal Transition (EMT) in several cancer types, including breast cancer, lung cancer, pancreatic cancer and prostate cancer [64–67]. ZEB1 expression in cancer cells is elevated upon signaling induction, including TGF-β and platelet-driven growth factor receptor-α signaling [68, 69]. As an activator of EMT, increased ZEB1 binds to E-boxes containing downstream targets, including E-cadherin and cell polarity factors, and represses their transcription by recruiting co-repressors CtBP or SWI/SNF chromatin-remodeling protein BRG1 [70, 71]. Notably, studies also reveal that ZEB1 can transcriptionally activate genes involved in TGF-β/BMP signaling through recruiting co-activators, p300 and P/CAF [72, 73]. In addition to its role in EMT, ZEB1 overexpression further contributes to EMT-related acquired resistance to epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKI) in non-small cell lung cancer through transcriptionally up-regulating E-cadherin, ST14 and vimentin [74]. Moreover, Yoshida et al., show that silencing ZEB1 expression restores sensitivity to EGFR-TKI, suggesting targeting ZEB1 could be a potential therapy to resensitize TKI-resistant tumors [74]. A recent study also reveals a novel role of ZEB1 in promoting angiogenesis in breast cancer [75]. The authors show that ZEB1 overexpression in breast cancer cells recruits Sp1 to VEGFA promoter region and activates VEGFA expression and secretion, therefore promoting angiogenesis in vitro and in vivo [75].
The tumor suppressor ZNFs in cancer progression
In addition to cancer promotion, several ZNFs have been found to function as tumor suppressors. For example, ZNF545, which is down-regulated in cancer cells as a consequence of promoter methylation, acts as a tumor suppressor by inducing cell apoptosis, repressing ribosome biogenesis and suppressing NF-kB and AP-1 signaling in nasopharyngeal, esophageal, lung, gastric, colon and breast cancer [76]. Notably, methylated degrees of five CpG sites (-232, -214, -176, -144 and -116) discriminate gastric cancer patients' survival outcome with higher CpG methylation predicting poorer overall survival [77]. Another ZNF known to be inactivated by promoter hypermethylation is ZNF331, also known as ZNF361 or ZNF463 [78–80]. Overexpression of ZNF331 inhibits cell growth by down-regulating genes, including DSTN, EIF5A, GARS, DDX5, STAM, UQCRFS1 and SET, and inhibits cell migration/invasion by down-regulating genes, including DSTN and ACTR3 [78].
ZNF24, also known as ZNF191 or Kox17, contains 4 Krüppel-like C2H2 zinc finger domains on C-terminus that function as DNA binding domains [81]. ZNF24 suppresses VEGF expression by binding to the proximal VEGF promoter, and negatively regulates tumor growth by inhibiting angiogenesis in breast cancer [82, 83]. Using transgenic zebra fish model, Jia et al., demonstrate that expression of human ZNF24 induces vascular defects, which can be recovered by VEGF overexpression [83]. Clinical studies of human breast cancer confirm the inverse correlation between ZNF24 and VEGF, indicating the tumor suppressor role of ZNF24 in breast cancer tumorigenesis by inhibiting angiogenesis [83]. Interestingly, a recent study shows that miR940 is up-regulated in gastric cancer and promotes gastric cancer migration and invasion by targeting tumor suppressor ZNF24 [84].
ZNF668 is a member of Krüppel C2H2 zinc finger protein family, which possesses 16 C2H2-type zinc fingers. ZNF668 facilitates p53 stabilization and activity by disrupting MDM2-mediated ubiquitination and degradation in breast cancer [85]. In addition, ZNF668 interacts with Tip60 to enhance H2AX hyperacetylation in response to ionizing radiation and promote RPA phosphorylation and recruitment to DNA damage foci upon UV damage, therefore leading to chromatin relaxation and loading of DNA repair proteins [86].
Zinc-fingers and homeoboxes-1 (ZHX1), which contains two C2H2 zinc finger motifs and five homeodomains, has been reported to be down-regulated in hepatocellular carcinoma and gastric cancer [87–89]. ZHX1 induces G1/S arrest through down-regulating cyclin D1 and cyclin E expression, and enhances apoptosis through down-regulating Bcl2 and up-regulating Bax and cleaved Caspase-3 [89]. Of note, Wang et al., demonstrate that miRNA, miR-199a-3p, targets ZHX1 for RNA degradation to promote cell proliferation and suppresses apoptosis in gastric cancer. Reconstitution of ZHX1 expression abrogates gastric cancer oncogenicity [88].
ZNFs: double-edged sword in tumorigenesis
Some ZNFs have been shown to play different roles in different cancer types and stimuli. For example, ZNF395 is overexpressed in various cancers, including Ewing sarcomas, osteosarcomas and renal cells carcinomas [90–92]. Moreover, ZNF395 expression is induced under hypoxic stress in glioblastoma, neuroblastoma and skin cancer [90, 93, 94]. Hypoxia-induced ZNF395 can transcriptionally up-regulate cancer-related genes and interferon-stimulated genes, such as IFIT1/ISG56, IFI44 and IFI16, in an IKK signaling-dependent manner [94]. These results implicate ZNF395 as a novel transcription factor which supports inflammation and cancer progression. However, a recent study reveals the tumor suppressor role of ZNF395 in liver cancer. The authors show that miR-525-3p, which is overexpressed in liver cancer, promotes liver cancer cell migration and invasion by targeting and down-regulating ZNF395 expression [95]. The clinical analysis indeed confirms the inverse correlation of miR-525-3p and ZNF395 in liver cancer [95]. These studies collectively show that ZNF395 may play different roles in different cancer types.
Kaiso, also known as ZNF348 or ZBTB33, belongs to the BTB/POZ subfamily of ZNFs. Kaiso can bind to sequence-specific or methyl-CpG DNA using its zinc finger motifs, while its N-terminus POZ domain helps homodimerization or heterodimerization with chromatin co-repressors, including nuclear receptor co-repressor I [96–98]. By recruiting chromatin co-repressors, Kaiso transcriptionally suppresses downstream gene expression. Kaiso was first identified as a tumor suppressor that transcriptionally suppressed oncogenic genes in sequence- or methyl-CpG-specific manner. For example, Kaiso represses cyclin D1 expression by binding to CCND1 promoter in a sequence- and methyl-CpG-specific manner in breast and colon cancer [99]. Since then, more and more studies have demonstrated the oncogene role of Kaiso in various cancers. For example, Kaiso is found highly expressed in triple negative breast cancers and involved in TGF-β-mediated metastasis by up-regulating several EMT genes, including Vimentin, Slug and ZEB1 [100]. High expression of Kaiso in prostate cancer promotes cell migration and invasion through transcriptional suppression of miR-31 expression in methyl CpG-specific manner [101]. In addition, Pierre et al., show that Kaiso transcriptionally suppresses HIF-1α expression by targeting to methylated HIF1A promoter in breast and colorectal cancer [102]. Kaiso is a versatile ZNF, which exerts different functions in different cell types in respond to different stimuli.
BTB, Broad-Complex, Tramtrack, and Bric-a-brac; C2H2, Cys2His2; EGFR-TKI, epidermal growth factor receptor-tyrosine kinase inhibitors; EMT, Epithelial-Mesenchymal Transition; ERC-1, erythroid complex; KRAB, Krüppel-associated box; mES, mouse embryonic stem; PCAF, p300/CBP associated factor; POZ, poxvirus and zinc finger; PTMs, post-translational modifications; SCAN, SRE-ZBP, CTfin51, AW-1 and Number 18 cDNA; TGF-β, transforming growth factor-β; ZFX, zinc finger protein, X-linked; ZHX1, Zinc-fingers and homeoboxes-1; ZNF, Zinc finger
This work was supported by Taiwan Ministry of Science grant 104-2627-B-006-001, the Aim for the Top University Project grant D105-35A07, and Taiwan Ministry of Health and Welfare grant 105-TDU-B-211-124-003.
This work was supported by Taiwan Ministry of Science grant 104-2627-B-006-001, the Aim for the Top University Project grant D105-35A07, and Taiwan Ministry of Health and Welfare grant 105-TDU-B-211-124-003 to YCW.
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Data and materials related to this work are available upon request.
Authors' contribution
JJ and YCW wrote the review. Both authors read and approved the final manuscript.
Competing interests
The authors declare that they have no competing interests.
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I'm torn a bit in even posting this. It's such a nightmare for the player and I can't really imagine going through something like this. But we'll show this as a lesson for others: know where your keeper is before you head the ball back to them.
Riley was trying to do the right thing. He wanted to make sure it got to Cannon - just put too much on it and Cannon was coming out at the same time.
Yeah, and I know the season just started, but were going to go ahead and hand the trophy to James Riley of the San Jose Earthquakes as the recipient of the own goal of the year. A miscommunication and a bit too much mustard on this header and he's got the own goal of the year in the bag.
In terms of importance though, he's got nothing on Liverpool's Riise.
San Jose is going to have a tough time getting out from under two tough losses in a row, and they play Houston next week in a sort of divorced family of a game.
His replacement in this game, Shea Salinas, just might have the biggest miss of the year too. That was in their home opener against Chicago.
However, what I like about Yallop is he stands by his players and they might just unite as the year goes on and come through and make the playoffs. I'm all for the underdog and with a couple wins this team could build up some confidence. That's all most teams need - it's all about putting a few wins together and suddenly your a good team again in the MLS.
Riley's just has to move on now. It's easier said than done, but the common refrain is that the best judge of character is when something goes wrong, how do those players respond. Or, what doesn't kill you makes you stronger. I don't think that Riley will have a problem overcoming this mistake. He seems like a good professional. It's a tough go for the player though, as this goal comes against his former team.
Riley is a hard worker and gets down the line well for an outside defender - let's hope he has a chance to redeem himself next week against Houston. And he can take heart; it happens to the best of them, just ask Riise how he feels, as his own goal essentially put Liverpool out of the Champions League final.
Quote of the Month:
"There is always someone out there getting better than you by training harder than you."
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