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Skip Hire Wickham Heath from YourLocalSkipHire.co.uk
YourLocalSkipHire.co.uk is Wickham Heath's number one skip hire firm. Working all over the UK, from Land's End to John O'Groats, we're able to provide fast, effective skip hire solutions to both businesses and individuals.
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Delhi to get 6 new city forests
New Delhi, May 18 (IANS) The national capital will get six new city forests by March 2019 under the green budget initiative of the Delhi government, officials said on Friday.
The officials of the Forest and Wildlife Department assured Environment Minister Imran Hussain, in a meeting organised to review the targets of the green budget, that the work will be completed by March 2019 after which the forests will be open for the public.
City Forest Management Committees will also be formed for supervision of the work and monitoring the maintenance by December 2018.
The officials also informed that over 20 lakh saplings will be planted across the city in the upcoming monsoon season.
The Delhi government will take the help of Resident Welfare Associations, Market Associations and non-government organisations for the campaign to be launched in June, the officials said.
"There are 14 forest nurseries in Delhi and the agencies will be given seedlings of local indigenous tree species from these nurseries free of cost," the Department said in a statement.
Hussain directed the Forest Department to develop more city forests expeditiously in the other parts of the city as well. He also appealed to the public to plant as many trees as possible for mitigating air pollution.

subset: commoncrawl


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text: The RTH 5.25 model is a simple rotating telescopic handler, but it is a high performing one. It can lift loads to a height of 25 m and is fitted with scissor stabilizers, which guarantee enhanced safety and stability during all operational manoeuvres. Scissor stabilizers are excellent for use in tight spaces. Even when only partially stabilized, the machine is designed to provide the best possible working load diagram on each side. When, instead, the stabilizers are fully extended, they ensure the machine performs to an incredibly high standard around 360°, thus allowing it to work on a very wide stabilized base. It is compatible with a vast range of accessories on account of its RFID system, which enables it to automatically recognise those accessories, creating the related working load diagrams accordingly. This telehandler is the idea solution to a long list of requirements in the construction and manufacturing sectors.


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Do you Ship Internationally ?
Yes we do ship internationally! When an order is placed on our site and you enter your address, you'll see the international shipping options and the site will automatically calculate the shipping costs for you.
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Please use the contact form which can be found on our Contact Us page.
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Should you need to track your order a Fedex or Post Office tracking reference will be made available on request.
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In the event that you no longer require the item you have ordered or you would like to change any particular item within your order, please contact us using the contact form or email email@example.com and we will do or best to change or cancel your order accordingly.
This, of course, may depend upon whether the item has already been shipped or not.
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Please Contact Us using the contact form or by email to email@example.com to arrange the return of a product. A note of your name, address, email address and reason for return should always be included with the product and returns can then be sent to the following address:
Pakatak Ltd, 12 Lincefield, Langdon Hills, Essex, SS16 6HS.
Please be aware, there is no shop, stock or over the counter facility at this location.
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Here at Pakatak Ltd we offer a no quibble 14 day money back guarantee on any purchase from us.
You must notify us that you would like to return your item within 7 days of purchase (via telephone or our contact us form) and you have 14 days from purchase date to return the item for a full refund of the product price (we do not refund the postage charges for any non faulty item returned).
Postage charges incurred returning the item to us will not be refunded under any circumstances.
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This cute and easy DIY Watermelon Soap can be made in just 15 minutes, and it smells delicious! A quick and easy homemade gift idea that's perfect for friends, family, teachers, and more!
This DIY watermelon soap is the cutest! I love these homemade soaps for their sweet summertime fragrance and because they are totally adorable, but what I love the most is the fact that I can whip up an entire batch of these beauties in under 15 minutes! Each layer will take a little longer to firm up, but the actual "work" part is done in less than 15 minutes. Easy peasy!
Supplies Needed for DIY Watermelon Soap
- Goat's Milk Soap Base (can also use Shea Butter Soap Base or Coconut Soap Base)
- Clear Soap Base
- Silicone Loaf Soap Mold
- Pyrex Measuring Cup
- Green Soap Colorant*
- Red Soap Colorant*
- Watermelon Fragrance Oil
- Poppy Seeds
* Note: I have been playing around with different types/brands of soap colorant lately, so for this watermelon soap, I used new-to-me supplies from Bramble Berry (a soap supply store). They worked wonderfully, and I highly recommend them; however, traditional red and green liquid soap colorants (available on Amazon.com or in local craft stores) will work as well!
For the Dark Green Layer: Cut 1/2 lb. of the goat's milk soap into cubes. Place the cubes into a 4-cup Pyrex measuring cup, and melt in the microwave in 30-second increments, stirring well after each. When the soap is completely melted, add 10 drops of watermelon fragrance oil and green soap colorant (as much as needed to achieve the desired color). Pour 3/4 of the green soap into the bottom of the soap mold and allow to firm for 15-20 minutes.
For the Light Green Layer: Take the remaining dark green soap (still in the Pyrex measuring cup) and add 1/4 lb. of white soap cubes. Melt in the microwave, stir until fully mixed (will be a light green color), and pour into the soap mold. Allow to firm for 15-20 minutes.
For the White Layer: Melt 1/4 lb. of white soap cubes in the microwave. Add 5-10 drops of watermelon fragrance oil, and pour into the soap mold. Allow to firm for 15-20 minutes.
For the Red/Pink Layer: Cut 3 lbs of clear soap base into cubes. Microwave until fully melted, then stir in 15-25 drops of watermelon fragrance oil and 1 tbsp. of poppy seeds. Pour into the soap mold, and sprinkle the top with a few more poppy seeds. Allow to firm for 90 minutes or until soap has completely hardened (time will depend on temperature and humidity)
Carefully remove the silicone liner from the wooden mold, and then remove the soap from the silicone.
This loaf mold makes an extra large batch of soap, so you can expect to get around 12-15 soaps from each batch.
Use a sharp knife to slice the soap into bars.
You can leave the rectangular soap as-is, or you can cut the corners as shown to create cute little triangle-shaped watermelon slice soaps!
The watermelon soap looks red upon an opaque background, but if you allow the light to shine through it, you'll see that it's actually a bright magenta pink!
They almost look good enough to eat! The watermelon fragrance is incredible – it smells just like Bubbleicious watermelon gum!
These DIY watermelon soaps are the perfect way to brighten up your summer, and the would make a great gift for friends, family, teachers…or anyone on your gift list!
If you like this DIY Watermelon Soap, don't forget to pin it!
You Might Also Like:


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Mmmm… some funky drumming, some stick manipulation, and perfect timing.
You might see a bit of dancing too…
Thanks to Uber Rob for the link. 🙂
Mmmm… some funky drumming, some stick manipulation, and perfect timing.
You might see a bit of dancing too…
Thanks to Uber Rob for the link. 🙂
Ken French (aka Toast) is the worlds fastest clapper.
The Ministry is pleased to announce a new member to our crew. Moon will be writing for us this!
Our good friend Mr. Om does some unspeakably beautiful contact juggling.
Turntablism session by DJ Kentaro, 2002 World DMC Champ.
Musically, I find it a bit too hecktik, but technically I really admire it.
Are those 3 silver contact balls above his mixer?
Steve Cousins sent us his latest masterpiece. First Neanderthals learn to use sticks, then Mankind lands on the moon and now Man climbs out of a big green balloon – that's progress.
I think this might be off topic…But it is Friday.
The first half of this clip is Rubber legs Al Norman dancing to Paul Whiteman's band. The clip is from The King of Jazz.
I think the Detours video project would have filed that one under "manifesting sh1t at a different time."
Jingluo user on youtube
Jingluo plays multiball in a style very differently to The CJ crowd, definately more on the meditation side of manipulation, than stage performance. His site Educated hands used to have the Chinese therapy ball theatre, I can't tell if it's live at the moment – its passworded.
Chinese Panda Bear Baoding Balls PT. 3– Palmspinning the formation [6.2]in one hand that's 8 balls in 1 hand.
Related Posts:
chinese therapy ball factory
china baoding balls stack and tai chi
80 ball Palmspinning – Liu Zhi


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Commuters beware: BART has 2-year plan to strengthen Transbay Tube
BART directors, hoping to prevent the flooding of the Transbay Tube during a catastrophic earthquake, voted Thursday to spend more than a quarter of a billion dollars to strengthen the tunnels beneath the bay.
But the seismic retrofitting, the second in five years, could affect BART service through the tube for more than two years. Trains would start weekday service at 5 a.m. — an hour later than now — and would share a single track through the tube from 9:30 p.m. until midnight, when trains would run less often.
The 3.6-mile tube, opened in 1974, is the heart of the BART system. It carries as many as 60,000 passengers an hour during the morning and evening weekday commutes. Built in 1969, the concrete-and-steel tube, connected on both ends by seismic joints, was considered virtually earthquake-proof. It survived the Loma Prieta earthquake in 1989, but engineers decided it could be made stronger.
BART bolstered the tube in 2013, as part of its systemwide seismic retrofitting program, and next plans to install interior steel liners that would prevent leaks or cracks caused by the worst imaginable quake from flooding the tube before people could evacuate.
"The Transbay Tube is structurally sound," said Alicia Trost, a BART spokeswoman. "It's been retrofitted to withstand a major earthquake."
But a devastating quake, the kind that happens once in a thousand years, could cause the tube's outer lining to crack and water to pour in more quickly than existing pumps could push it out. In addition to the installation of interior steel linings that would keep water out, the retrofit plan calls for contractors to install more powerful pumps.
The project would not come without pain for commuters, however. To give construction crews an additional hour to work on the project, BART wants to delay start of service in the mornings. Instead of starting at 4 a.m. BART service would begin at 5 a.m., inconveniencing about 2,600 riders.
Thousands more would see service cut at night when BART would restrict trains to a single track for the last three hours of service. Train frequency would also be reduced during that period, from every 20 minutes to every 24 minutes.
To make up for the morning shutdown, which surveys show will disparately affect low-income and minority passengers, BART is considering running buses, shuttle trains or a combination.
Since the work is not scheduled to start until June 2018, BART doesn't need to make a decision. But directors agreed that they should consider the replacement service during the coming summer.
Directors awarded the $267 million contract to a joint venture of Shimmick and CEC contractors. Money to pay for the project comes from earthquake safety bonds that voters approved in 2004.
Michael Cabanatuan is a San Francisco Chronicle staff writer. Email: firstname.lastname@example.com Twitter: @ctuan


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We are UK memorial suppliers and have a vast experience of catering to memorial industry for over 17 years. We supply tombstones in a vast range of colours,textures,shapes,finishes and sizes.
We have a strong support of manufacturing memorials by our indian company who also have a consistent supply of premium indian jet black material.We are built on strong values and can be trusted on quality supplies and good service.Our stock yard in Gainsborough, Lincolnshire has a good collection of memorials for quick delivery. We welcome you to our stock yard to see the memorials.


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Thinking of removing AnthroKai pages...
I wanna do a semi-remastered of my comic Anthro Kai.
Reason for this. I haven't update in forever and people might think is discontinue ,dead.
If re-release them again it might attract new viewers
like if it were anew comic. If i do this it will give me the time to fix the pages, I wont be re-drawing the pages. I'll just fix, text grammar, little story plot, remove bad dialogue and add better , fix the art in the pages if needed and remove unwanted pages. migth even rename the comic.
Honestly the comic i was making it as i go. So if i do this i can finally have a concrete plot and don't have to be so inconsistent. The pages wont be conflicted by this. Because i bet non of my viewers have a clue what the comic is about <____>
I'm thinking of this. But i wanna know what you fans think? Should i do it? Yes or no?
And this is not a April Fool joke.
- Posted by Raxki Yamato on April 1st, 2015, 12:19 am - 3 comments
Reader Comments:
April 1st, 2015, 4:53 am
go for it
Posted by GabrielsThoughts
April 1st, 2015, 6:29 am
I think only one artist went out of their way to actually remaster their comic after it had been published. And, that was the genius behind Tintin... although I've also heard the artist behind charby the vampirate has taken a stab at it in recent years, and I've seen older versions of a british comic that had less life in the reboot than in the perfunctory original. So, either way, It's up to you.
Do it.
Posted by Meleeman
June 5th, 2015, 8:13 pm
I think that it would certainly help if you did. I'm planning a relaunch myself so. just make sure you continue with the story bro.
if you're recreating the story then remove teh old pages. if you're adding on to it/editing it. then keep the old pages. save it for later and finish the story.


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Story highlights
- Courageous people are scared to the core, but they just make themselves go forward anyway
- The longer you analyze it, the harder it gets because then your mind gets involve
- Write down a list of times when you acted braver than you thought you could
1. Don't Turn an Excuse into an Identity
We need be careful of how we label ourselves, for example, saying "I'm an fearful person" or "I'm a weak person" or "I'm not a strong person." Usually that label comes from your current or past behavior, but once the label becomes a part of you, it starts to control you.
So what I try to get people to understand is: Hey, you're not broken. You don't need to be fixed. You don't need a label. All you need to do is say, "I have to decide how I want to be now going forward."
2. Develop a habit
Courage is not the absence of fear. That idea is the biggest b.s. in the world. Fear is impossible to eradicate. If you were completely fearless, you'd be dead.
People who are courageous are scared to the core -- they just make themselves go forward anyway; they make themselves take some kind of action. Taking action, even though you're afraid, is how you become courageous -- because courage, like fear, is a habit.
The more you do it, the more you do it, and this habit -- of stepping up, of taking action -- more than anything else, will move you in a different direction.
3. Let your body lead the way
Taking that action for the first time can be pretty rough. When it comes time to give the speech to the committee or snowboard down the mountain, don't hesitate. Don't start to analyze it. The longer you stand there, the harder it gets, because then your mind gets involved.
If your challenge is mental, use your body. If it's in the body, use your body more aggressively. At 17 years old, I was on my own, sleeping in a laundry room. I had no idea what to do. I was so depressed. Fear is physically debilitating.
I had to defeat it. So I made myself run until I thought I was going to spit up blood. I got stronger in my body, which, in turn gave me mental strength.
4. Write your list
Everyone has stages in their life when they have been courageous. It could be in a relationship or in a job. It could be as simple as negotiating with a car salesman.
Write down a list of these moments, times when you acted braver than you thought you could. Some of these you may take for granted because you didn't recognize them as courage at the time; you were merely doing what had to be done. Others may surprise you.
But once you look at them all together, there's always a pattern. You got obsessed with something you really wanted. Or you were concerned for another person. Or you knew you had the skills. You might have been scared to death, but you got up and did it.
And here's the secret: Once you see the common denominator, you start to realize, "I know what motivates me. I can do this again, in different situations." Your use of courage ripples out. You start applying it in more and more areas of your life.
5. Remember to stretch
If you want to live a life that's courageous, you've got to stretch, and to stretch means: When I can't, I must. Every time you say, "I can't do it," you're going to immediately say, "I must do it." This is a simple idea.
I heard it first at age 16 from a close family friend named Art Williams. At the time, I asked, "Does that mean if I can't jump off the cliff, I must go ahead and jump?"
He said, "You're not a stupid person, Tony. Don't be stupid. It means if you find yourself saying I can't do something, but you know it in your heart of hearts that if you do it you're going to grow, you're going to be a better person, it's going to contribute to your family or to your kids or to something that matters, and you keep saying I can't do it, there is no question -- you must do it. You don't discuss it anymore. You just take immediate action. You make the phone call. You step up in front of the room. You raise your hand. You do what's necessary." And I said, "That's not a very safe life." And he said, "If you want safety, go to prison. If you want a fulfilled life, you've got to step up."


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When you need pest control in Brampton and the GTA, ON, call on Star Pest Control Inc., who has been serving the community since 2009. For many years we have been offering expert residential pest control including Pest Control, Bug Removal, Bed Bug Removal and much more. We strive to keep our clients satisfaction as our top priority. When you have bugs, roaches or wasps at your home or business in Brampton, ON, call on Star Pest Control Inc today.
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Subject: I have found a DVD that I think you would enjoy
|Twilight in the Sierras
Actors: Roy Rogers, Trigger, Dale Evans, Estelita Rodriguez, Pat Brady
Director: William Witney
Genres: Westerns
NR 2003 1hr 7min
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Movie Details
Similar Movies
Movie Reviews
"Roy Rogers B-Western Series ... Twilight in the Sierras (19
J. Lovins | Missouri-USA | 01/08/2008
(5 out of 5 stars)
"Republic Pictures presents "TWILIGHT IN THE SIERRAS" (22 March 1950) (64 mins/B&W/Color) (Fully Restored/Dolby Digitally Remastered) --- Roy Rogers (born Leonard Slye) moved to California in 1930, at the age of 18 --- played in such musical groups as The Hollywood Hillbillies, Rocky Mountaineers, Texas Outlaws and his own group, the International Cowboys --- In 1934 he formed a group with Bob Nolan and Tim Spencer called "Sons of the Pioneers" --- While in that group he was known as Leonard Slye, then Dick Weston and finally Roy Rogers --- Their songs included "Cool Water" and "Tumbling Tumbleweeds" --- They first appeared in the western "Rhythm on the Range" (1936), starring Bing Crosby --- In 1937 Roy went solo and made his first starring film in 1938, "Under Western Stars" (1938) --- He made almost 100 films --- then came television, "The Roy Rogers Show" (1951) ran on CBS from October 1951 through September 1964.
Under William Witney (Director), Edward J. White (Producer), Sloan Nibley (Screenwriter), John MacBurnie (Cinematographer), Sid Robin (Songwriter), Foy Willing (Songwriter), Stanley Wilson (Composer (Music Score), Tony Martinelli (Editor), Frank Hotaling (Art Director), John McCarthy (Set Designer), James S. Redd (Set Designer), Adele Palmer (Costume Designer), Howard Lydecker (Special Effects), Theodore Lydecker (Special Effects) - - - - Our story line and plot, In a gangster plot set in the West, Roy, as a marshal investigates a gang of counterfeiters who have kidnapped an ex-convict with the aim of making him engrave the plates --- Screenwriter Sloan Nibley spices up the plot with a side story about a mountain lion hunt --- At one point Trigger is wounded and has to be nursed back to health by Pat, who is somewhat of a doctor for both animals and humans --- The Sons of the Pioneers are not around but a similar group called the Riders of the Purple Sage do a good job filling in for them --- And Roy gets to sing a song or two. So "Twilight in the Sierras" should delight Roy and Dale's many fans --- Must recommend the Happy Trails DVD's to you --- Each one has new information about Roy Rogers and Dale Evans and each actor or director in the film --- If you are a Roy Rogers and Dale Evans fan like me you will totally enjoy all the extra footage showing Roy and Dale going to parades and visiting children in hospitals, the story of Roy proposing marriage to Dale, and more inside info also about the family --- Each film starts out with Roy and Dale seated on a small stage back around 1987 talking about the sagebrush films they are showing --- some wonderful tunes, ROOTIN TOOTIN COWBOY FROM THE WEST -- PANCHO'S RANCHO -- IT'S ONE WONDERFUL DAY -- TWLIGHT IN THE SIERRAS.
the cast includes
Roy Rogers ... Roy Rogers
Trigger ... "Smartest Horse in the Movies"
Dale Evans ... Pat Callahan
Estelita Rodriguez ... Lola Chavez
Pat Brady ... Dr. Sparrow Biffle DVM
Russ Vincent ... Ricardo Chavez
George Meeker ... Matt Brunner
Fred Kohler Jr. ... Mason (Brunner henchman)
Edward Keane ... Judge Wiggins
House Peters Jr. ... Jim Williams
Pierce Lyden ... Henchman Blake
Don Frost ... Bartender
Joseph A. Garro ... Sy (henchman)
William F. Leicester ... Paul Clifford (as Wm. Lester)
Foy Willing ... Foy (Leader, Riders of the Purple Sage)
Riders of the Purple Sage ... Singing Ranch Hands
Bob Burns ... Townsman
Cactus Mack ... Townsman
Johnny Paul ... Cowhand (Riders of Purple Sage)
Jack Perrin ... Townsman
John Pickard ... Henchman
Darol Rice ... Riders of the Purple Sage Band Member
Al Sloey ... Riders of the Purple Sage Band Member
Harry Strang ... Sheriff Callahan
Robert J. Wilke ... Bus driver
1. Roy Rogers (aka: Leonard Franklin Slye)
Date of Birth: 5 November 1911 - Cincinnati, Ohio
Date of Death: 6 July 1998 - Apple Valley, California
2. Dale Evans (aka: Frances Octavia Smith)
Date of Birth: 31 October 1912, Uvalde, Texas
Date of Death: 7 February 2001, Apple Valley, California
3. William Witney (Director)
Date of Birth: 15 May 1915 - Lawton, Oklahoma
Date of Death: 17 March 2002 - Jackson, California
Check out a new book from Empire Publishing - "THE ROY ROGERS BOOK: A REFERENCE TRIVIA SCRAPBOOK" (Paperback) --- reference trivia scrapbook of Roy Rogers written by Western film historian David Rothel whose accounts of thrilling adventures of B-Western heroes during the Saturday matinees of yesteryear takes us back to our childhood, family and friends --- this is a wish come true, reliving those wonderful years from the past through the pen of David Rothel --- Roy was a top box office draw for Republic Pictures when you went to see him on the big screen, you got exactly what the marquee said --- plenty of thrills, action and hard riding with a song or two thrown in for good measure --- Elected to the Country Music Hall of Fame in 1980 as a member of the "Sons of the Pioneers" and elected again in 1988 as Roy Rogers "King of the Cowboys" --- Roy got his horse "Trigger" in 1938 and rode him in every one of his films and TV shows after that --- "Trigger" died in 1965 age of thirty-three --- Roy's dog's name was "Bullet" and appeared in almost as many of his films as "Trigger" did --- Roy's theme song, "Happy Trails", was written by Queen of the West and his wife Dale Evans --- inducted (with his wife Dale Evans) into the "Hall of Great Western Performers of the National Cowboy and Western Heritage Museum" in 1976 --- inducted as a member of the "Sons of the Pioneers into the "Hall of Great Western Performers of the National Cowboy and Western Heritage Museum" in 1995 just three years before his death --- Don't miss this one --- now appearing on Amazon and Empire Publishing --- Don't hesitate - rush out and pick up your copy today --- Great reading in the days and weeks to come --- I guarantee it!
Hats off and thanks to Les Adams (collector/guideslines for character identification), Chuck Anderson (Webmaster: The Old Corral/B-Westerns.Com), Boyd Magers (Western Clippings), Bobby J. Copeland (author of "Trail Talk"), Rhonda Lemons (Empire Publishing Inc) and Bob Nareau (author of "The Real Bob Steele") as they have rekindled my interest once again for Film Noir, B-Westerns and Serials --- looking forward to more high quality releases from the vintage serial era of the '20s, '30s & '40s and B-Westerns ... order your copy now from Amazon where there are plenty of copies available on DVD --- stay tuned once again for top notch action mixed with musical adventure --- if you enjoyed this title, why not check out Amazon where they are experts in releasing B-Westerns --- all my heroes have been cowboys!
Total Time: 64 mins on VHS/DVD ~ Republic Pictures ~ (6/24/2003)"


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- Research
- Open Access
Evidence for regulatory diversity and auto-regulation at the TAC1 locus in sensory neurones
© Shanley et al; licensee BioMed Central Ltd. 2011
- Received: 16 November 2010
- Accepted: 4 February 2011
- Published: 4 February 2011
The neuropeptide substance-P (SP) is expressed from the TAC1 gene in sensory neurones where it acts as a key modulator of neurogenic inflammation. The promoter of TAC1 (TAC1prom) plays a central role in the regulation of the TAC1 gene but requires the presence of a second regulatory element; ECR2, to support TAC1 expression in sensory neurones and to respond appropriately to signalling pathways such as MAPkinases and noxious induction by capsaicin. We examined whether the effect of capsaicin on ECR2-TAC1prom activity in larger diameter neurones was cell autonomous or non- cell autonomous. We demonstrate that TRPV1 is not expressed in all the same cells as SP following capsaicin induction suggesting the presence of a non-cell autonomous mechanism for TAC1 up-regulation following capsaicin induction. In addition, we demonstrate that induction of SP and ECR1-TAC1prom activity in these larger diameter neurones can be induced by potassium depolarisation suggesting that, in addition to capsaicin induction, transgene activity may be modulated by voltage gated calcium channels. Furthermore, we show that NK1 is expressed in all SP- expressing cells after capsaicin induction and that an agonist of NK1 can activate both SP and the transgene in larger diameter neurones. These observations suggest the presence of an autocrine loop that controls the expression of the TAC1 promoter in sensory neurones. In contrast, induction of the TAC1 promoter by LPS was not dependent on ECR2 and did not occur in large diameter neurones. These studies demonstrate the diversity of mechanisms modulating the activity of the TAC1 promoter and provide novel directions for the development of new anti-inflammatory therapies.
- Dorsal Root Ganglion
- Capsaicin
- Sensory Neurone
- Dorsal Root Ganglion Neurone
- Autocrine Loop
The neuropeptide substance P (SP), encoded by the Tachykinin 1 (TAC1) gene, acts as a key regulator of neurogenic inflammation [1, 2]. Consistent with this role SP is usually expressed in nociceptive C-fibre sensory neurones within the dorsal root ganglia (DRG) [2]. In addition, it has been established that the expression of SP is up-regulated in larger diameter A and B fibre sensory neurones following noxious stimulation, an expression pattern also associated with the development of inflammatory pain and hyperalgesia [3, 4]. Therefore, gaining a better understanding of the mechanisms involved in controlling the expression of the TAC1 gene in sensory neurones will be critical to understanding the mechanisms responsible for exacerbating the distressing symptoms of many forms of chronic inflammatory disease.
TAC1 gene expression, in common with the inflammatory pain response, is modulated by a number of different stimuli such as bacterial infection and noxious stimulation that often results in depolarisation of sensory neurones. Accepted paradigms for each of these processes include bacterial wall lipopolysaccharide (LPS) [5, 6] capsaicin [7–10] and potassium-induced cell depolarisation that have all been shown to modulate TAC1 promoter (TAC1prom) activity [11, 12]. Because of the critical role that TAC1 plays in neurogenic inflammation we explored whether there was any commonality in the mechanisms that influence the activity of TAC1prom in different populations of sensory neurones following stimulation by capsaicin, LPS or potassium.
In addition to noxious stimulation capsaicin treatment has been widely used as a model system in the study of hyperalgesia [13, 14]. Capsaicin activates the transient receptor potential cation channel, subfamily V, member 1 (TRPV1) a major integrator of noxious stimuli that is expressed on a subset of SP expressing DRG neurones [7–10]. Capsaicin has also been shown to induce expression of TAC1 in vivo [9, 10] and gene deletions of the SP-receptor (NK1) significantly reduces the hyperalgesic affects of capsaicin [15]. We have previously shown that capsaicin could induce the activity of TAC1prom but only in the presence of the evolutionary conserved region 2 (ECR2) enhancer [16]. This synergy could also be induced by activation of mitogen activated protein kinase (MAPkinase) pathways and the effects of capsaicin on ECR2-TAC1prom interaction could be blocked by antagonism of mitogen-activated protein kinase kinase (MEK kinase) [16]. We also revealed that stimulation of sensory neurones by capsaicin triggers ECR2- TAC1prom synergy in larger diameter sensory neurones; an expression of SP associated with hyperalgesia [16]. However, we were unable to determine whether the effects of capsaicin were cell autonomous or cell non- autonomous i.e. were the mechanisms controlling the up-regulation of TAC1prom only found within TRPV1 expressing cells or did these mechanisms involve communication between cells. Furthermore, if the effects of capsaicin are non-cell autonomous it is important to determine the communication pathways that induce ECR2-TAC1prom activity in large diameter neurones. In addition, because TRPV1 activation depolarises sensory neurones we also asked how specific the TRPV1-ECR2-TAC1prom relationship was and whether other methods of cell depolarisation such as potassium induced depolarisation, could induce ECR2-TAC1prom synergy [17, 18].
Bacterial LPS has also been shown to upregulate the expression of TAC1 [5, 6] and deletion of the TAC1 gene in mice reduces the effects of bacterial sepsis and LPS induced organ damage [19]. LPS is a potent activator of both the inflammatory response and the immune response through its receptor, Toll Like Receptor 4 (TLR4) [20]. It has been shown previously that activation of the TLR4 receptor triggers MAPkinase or NFκB dependent pathways [21]. However, it is not known whether TLR4 is expressed on sensory neurones, whether the MAPkinase of NFκB pathways modulate the effects of LPS on TAC1 expression, whether the effects of LPS is cell autonomous or whether the ECR2 enhancer is required for TAC1prom activation by LPS.
Using primary cell culture and transgenic explant culture we examined the mechanisms through which capsaicin, potassium mediated cellular depolarisation and LPS modulate TAC1prom activity within sensory neurones. Our findings demonstrate surprising diversity in the mechanisms modulating the activity of the TAC1 gene in sensory neurones. Identifying this diversity, as well as identifying of the components of the different mechanisms involved, will have an important baring on the future development of more targeted inflammatory pain therapies and will represent an important step in understanding the processes that regulate gene expression during the inflammatory response.
Primary cell culture and transfection
Neonatal rat dorsal root ganglia (DRGs) were cultured as described previously [22] except for the following modifications. Following dissociation, DRG's neurons were re-suspended in Amaxa transfection solution. 100 μl of the cell suspension was added to a microcentrifuge tube containing 2 μg of plasmid DNA before being placed in an Amaxa cuvette and transfected using a nucleofector device set at program G-013. 100 ul of culture media (consisting of Hank's F12 meida (Gibco), putrescine, transferrin, BSA, progesterone, sodium selenite, FGF (20 μg/ml), insulin (100 μg/ml) and antibiotics) was added to the transfected cell suspension before being transferred into a culture dish containing 0.3 ml of culture media. Cells were left in the incubator and allowed to adhere to poly-L-lysine/laminin coated glass cover slips. Immediately after Amaxa transfection with DNA DRG neurons were cultured for 24 hours in a solution containing respective agonists or antagonists (See below). After culture, cells were fixed with 4% paraformaldahyde and expression of the LacZ gene (βgal) was visualised by staining with X-gal stain (5 mM K3Fe(CN)6; 5 mM K4Fe(CN)6; 2 mM MgCl2; 1 mg/ml X-gal) for two hours. The number of blue DRG neurons as a percentage of the total number of neurons was assessed by cell counting on an inverted DIC microscope. Proportions are adjusted relative to a control plasmid containing the CMV promoter that was transfected at the same time to normalise transfection efficiencies between different batches of rat neonates.
Transgenic DRG explant analysis and immunocytochemistry
Whole DRG explants were dissected from ECR2-TAC1prom-LacZ transgenic mouse neonates and placed in the same culture conditions as described above. These explants were then treated with agonists or antagonists for 24 hours (see below), fixed in 4% paraformaldehyde and incubated with 30% sucrose in optimal cutting temperature (OCT) media overnight. Sections were cut with a cryostat to 10 μm thickness, permeabilised with 0.1% SDS for 5 mins then incubated in 10% foetal calf serum in tris-buffered saline with 1% triton (TBST) for 10 mins. Sections were washed 3 times for 5 mins in TBST and treated sequentially in primary antibodies overnight (rabbit-anti-βGal, 1:200; rat-anti-Substance P, 1:200 (AbCam) Goat-anti Toll-Like-Receptor 4 (TLR4) or rat anti-NK1 (1:200, Santa Cruz Biotechnology). Antibodies were visualised by incubation with the appropriate secondary antibody (diluted to 1:250) for 40 mins at room temp (goat-anti-rat Texas red, donkey-anti-Rabbit ALEXA 488 or Donkey-anti-goat ALEXA 488, all from Molecular Probes).
Agonists and antagonists
Lipopolysaccharide, purchased from Sigma-Aldrich, was dissolved in PBS and used at a concentration of 10 μg/ml [23]). Capsaicin, purchased from Sigma-Aldrich, was dissolved in EtOH and used at a concentration of 10 μM [24]. Forskolin, obtained from Sigma, was dissolved in DMSO and used at a concentration of 10 μM [25]. [Sar9, Met(O2)11]-Substance P was obtained from Tocris, dissolved in H2O and used at a concentration of 100 nM as recommended by Tocris. KCl was obtained from Tocris, dissolved in H2O and used at a concentration of 30 mM [26].
Details of the production of the constructs used in the current study have been described previously [16].
Image capture and analysis
Images were captured on Qicam monochrome camera mounted on a Nicon Eclipse 400 fluorescence microscope and processed using Improvision Velocity software v4.1. Figures were assembled on Adobe Photoshop CS3 software.
Statistical analysis
All experiments were repeated a minimum of three times on separate dates using separate groups of animals (n ≥ 3). Two tailed student t-tests were used to test the significance of data derived from primary cell cultures. Unpaired t-tests were used on data derived from transgenic explants studies to test the significance of the changes in the proportion of expressing cells within a specific cell diameter range. Statistical analysis was done using Microsoft Excel.
Induction of TAC1 by capsaicin does not occur in all TRPV1 expressing cells
The ECR2-TAC1prom transgene can be activated by cell depolarization
We have previously used luciferase assays to determine how TAC1prom responds to various signalling antagonists and agonists and its functional relationship with other regulatory elements [16]. However, although luciferase assays are very sensitive in showing changes in the expression of genes from large population of cells they are limited in their ability to give information on either the identity of these cells in mixed populations or on the numbers of cells expressing the reporter construct. This is an important consideration as previous studies have shown that, in addition to increasing levels of gene expression [7–10] capsaicin also increases the expression of the TAC1 gene in larger diameter neurones [3, 4, 27–31]. In order to address this problem we have previously combined luciferase assays with cell counting assays using the LacZ gene as the reporter. For example, using luciferase assays in heterogeneous primary DRG culture we show that upregulation of the activity of TAC1prom by capsaicin or the MAPkinase agonist angiotensin only occurs in the presence of the remote ECR2 enhancer [16]. These quantitative observations were supported by parallel experiments carried out with a LacZ marker which showed that in the presence of ECR2, TAC1prom responded to angiotensin and capsaicin by driving the expression of the LacZ marker in significantly greater numbers of cells [16].
We also recovered intact DRG explants from neonate mice transgenic for the ECR2-TAC1prom-LacZ construct and cultured these cells in the presence and absence of 30 mM KCl [26]. We detected a significant increase in the number of cells with a diameter greater that 15 microns expressing the transgene mirroring the effects previously observed using capsaicin and angiotensin [16] (Figure 2C).
Capsaicin can induce the expression of the NK1 receptor in DRG neurones that also express SP
Activation of the NK1 receptor induces expression of the ECR2-Tac1prom-LacZ transgene
Induction of TAC1prom by LPS is independent of ECR2
Neurogenic inflammation has been shown to play a critical role in the progression of diseases such as asthma, inflammatory bowel disease and arthritis [43]. Thus, gaining a better understanding of the mechanisms controlling the activity of TAC1prom has been a continuing priority since the discovery of the involvement of the TAC1 gene in neurogenic inflammation. We have previously demonstrated the novel requirement by TAC1prom for the presence of the ECR2 enhancer to respond appropriately to MEKKinase activation and noxious induction by capsaicin [16]. In the current study, we have used a combination of transgenic analysis, DRG explant culture and primary cell line transformation to further examine the effects of well characterised inflammatory mediators such as capsaicin and LPS on TAC1 promoter activity. We first demonstrated that the expression of TRPV1 did not change significantly in response to capsaicin and that many of the larger diameter neurones, that expressed substance-P in response to capsaicin, did not express TRPV1. These observations suggest that the induction of TAC1 promoter activity in larger diameter neurones by capsaicin is not cell autonomous. Furthermore, we demonstrate that depolarising DRG neurones with potassium was also able to induce expression of the transgene in large diameter neurones providing further evidence that the previously observed relationship between TRPV1 and the transgene depends on cell depolarisation. However, we also suggest that the induction of ECR2-TAC1prom synergism by potassium induced depolarisation might also activate voltage gated calcium channels (VGCC) that are also known to play a role in pain modulation in sensory neurones [44]. These observations support the hypothesis that TRPV1 is only one of many pathways that can stimulate synergy between ECR2 and the TAC1prom.
Because a number of previous studies have reported the expression of NK1 in these neurones, and also report on the possibility of an NK1-TAC1 autocrine loop, we next explored the possibility that the NK1 receptor may be involved in the induction of TAC1prom in DRG neurones [34, 35, 37, 38]. In support of these observations we were able to show a parallel induction of NK1 and SP in larger diameter DRG neurones following capsaicin treatment. Significantly, we were also able to observe that treatment of ECR2- TAC1prom-LacZ transgenic DRG explants with an NK1 agonist triggered the expression of the transgene in large diameter neurones in a similar way to capsaicin. These observations are consistent with the proposition that stimulation of large diameter neurones with SP can induce the expression of SP within these neurones and induce an autocrine loop. The presence of an TAC1 gene driven autocrine loop in these large diameter neurones suggests a possible mechanisms for the induction and maintainance of hyperalgesia which is associated with the expression of SP in large diameter neurones[3, 4]. However, it must be recognised that [Sar9, Met(O2)11]-SP can also bind a number of other receptors, including NK2 and NK3, although with greatly reduced affinity [45]. Therefore, whilst there is a strong case for the involvement of NK1 in the maintenance of TAC1prom activity in large diameter neurones following capsaicin treatment we are also aware that NK1 may not be the only receptor responsible and that redundancy with other receptors, such as NK3, is a possibility. In addition, although we present evidence for the presence of an NK1-ECR2-TAC1prom driven autocrine loop in the perpetuation of SP expression in large diameter neurones there is the possibility that another pathway, which does not involve NK1 signalling, is involved in the initial up-regulation of the NK1 gene expression in large diameter neurones
Following stimulation with KCL or capsaicin it was observed that the expression of the ECR2-TAC1prom-LacZ transgene or the NK1 receptor respectively were observed in significantly lower proportions of small diameter neurones. However, we have little evidence that the numbers of small diameter neurones expressing these markers decrease significantly after treatment. Therefore, these observations do not constitute a significant change in the numbers of cells expressing the respective marker but instead reflect a reduction in the overall proportion of small diameter cells expressing the marker as a result of the increased proportion of expressing larger diameter neurones.
LPS was also shown to increase the numbers of primary neurones in which TAC1prom was active. However, in contrast to angiotensin and capsaicin [16] induction of TAC1prom by LPS did not depend on the presence of ECR2. In addition, we were unable to detect any evidence for the upregulation of the TAC1prom transgene in neurones greater that 15 microns in diameter in DRG explants exposed to LPS. Our data suggests that activation of TAC1prom by LPS is via an unidentified cell autonomous mechanisms that does not require the presence of ECR2 (step 11). We have previously shown that angiotensin II is unable to activate TAC1prom in the absence of ECR2 [16] Although LPS binding to TLR4 has been shown to activate MAPkinase pathways the lack of requirement by LPS for ECR2 in the induction of TAC1prom, in combination with the inability of MAPkinase to induce TAC1prom in the absence of ECR2, suggests that LPS does not control TAC1prom through MAPkinases. Thus, by deduction, LPS is more likely to modulate TAC1prom activity through NFκβ than MAPkinase pathways [21]. In keeping with this hypothesis there have been previous reports of highly conserved NFκβ binding in TAC1 exon 1 [48] and we have used bioinformatics to detect the presence of NFκβ binding sites close to the human TAC1 transcriptional start site (data not shown).
Despite evidence showing that LPS and capsaicin can activate TAC1 expression and inflammation the current study suggests a distinction between the signal transduction pathways that control either capsaicin or LPS activation of TAC1prom. In hindsight, the basis for these differences make sense as, despite both being able to activate the promoter, they are both known to operate through different signalling pathways [13, 21]. Further analyses of the influence of NFκβ on activation of TAC1prom in sensory neurones exposed to LPS will be very exciting.
The current study sheds light on the fascinating diversity of regulatory mechanisms that control the activity of TAC1prom during challenges by inflammatory mediators such as LPS and capsaicin and provides further evidence for the existence of an auto-regulatory loop that controls the expression of the TAC1 gene [34, 35, 37, 38]. Gaining a better understanding of the diverse mechanisms that control the expression of TAC1prom during the inflammatory response will take us a step nearer to the development of more specific and novel therapies for the treatment of conditions such as hyperalgesia and inflammatory pain.
This project was funded by The Tenovus Trust, Scotland, the BBSRC (BB/D004659/1) and the Wellcome Trust (080980/Z/06/Z). SD was funded by a BBSRC strategic studentship (BBS/S/2005/12001).
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Job: Science Presenter for National
Physics of Skateboarding Assembly Tour
National Travel, Based in Philadelphia, PA and Oradell, NJ
We want YOU for Science/Engineering Education Outreach. Travel the country presenting exciting school assemblies where audiences truly LEARN. Crash into the physics of safety by lying across a bed of nails. Find the physics of balance by levitating students. Make pigs fly to explore how big-air tricks and jumps work. Bring the science of the Ollie to life!
Based in Philadelphia, Wondergy uses science to educate, change attitudes, and affect behavior. The Wondergy team brings knowledge, experience, and personal excitement about science to create exciting, inviting interactions that empower people to learn about the phenomena that matter to them. Through promoting learning, we build a stronger workforce, smarter consumers, and more informed stewardship of our world.
For academic year 2016-17, we are proud to launch Sk8Sci: The Physics of Skateboarding nationally. This is a full time job, requiring a lot of car travel. We provide all of the equipment for travel and demonstration, plus training and general support.
- Present 7-11 45-minute assembly programs each week for 100-500 students per show
- Guide inquiry-driven science exploration; encourage questioning and discovery
- Share your excitement about science and engineering with diverse communities
- Pack, setup, re-pack science demo and sound equipment
- Communicate with schools to coordinate arrival and setup
- Drive from school to school – this tour will involve substantial driving around the country
- National Tour begins Late September and ends Late May or Early June
- Occasional training and occasional shows starting spring 2016 – scheduled by your availability and ours
- Full time tour – Fall in the eastern US, Spring in the west, with substantial time on the west coast.
- Breaks for Thanksgiving and Christmas/New Year's. Some flexibility in tour schedule and breaks are possible.
- Email lesson plans and science information to each school you visit
- No sales duties. You'll be sent a schedule each week.
- Possible local and international travel when not on national tour
Qualifications of an Ideal Candidate
- Science or Engineering background strongly preferred
- Comfortable with electronics and sound production technology
- Charismatic and passionate, with strong communication skills.
- Creative and able to improvise; you should welcome audience questions, ideas, and jokes!
- Comfortable in front of large audiences – performance, teaching, or public speaking experience preferred
- Able to articulate complex concepts in a simple yet accurate manner
- Comfortable with basic physical science concepts relating to static physics, gravity, momentum, and projectile motion
- Clean, valid drivers' license and background check
- Able to lift and carry over 50lbs
How to Apply
Email us your resume and cover letter, and a short video telling us why you'd be awesome for this (links are fine). We will have a phone interview, which may be followed by an in-person "audition".
Check out http://wondergy.com/tour for more information on our current tours.
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Friday, 15 February 2008
what I gave my Valentine...
Nope, not oysters.
No, not even a slap-up meal of prime steak.
None of those.
Give up?
OK, then - it was...
... crabs.
NO! Not those crabs. (How could you even begin to think that?)
Sadly, I can't claim to have prepared the crabs myself, but I did at least put them on the plate and make the parsley and lemon dressing to go with them.
And yes, they were very much appreciated. ;)
18 comments:
- Manggy said...
Haha! That is naughty. I wasn't thinking it till you brought it up!
Frankly, I'd rather have crabs than steak any day.. :)
- 16 February 2008 at 10:49
- Pixie said...
LOL :) They look delicious and I'm sure they were very much appreciated!
- 16 February 2008 at 12:40
- Bellini Valli said...
Seafood would be the ultimate on Valentine's Day. I'm sure that they were appreciated :D
- 16 February 2008 at 15:10
- lorraine@italianfoodies said...
hahaha!! They look great, nice presentation!
- 17 February 2008 at 14:51
- Rosie said...
What a great dish for Valentines Day! I bet every bite was savoured too :)
Rosie x
- 17 February 2008 at 15:02
- eatme_delicious said...
Beautiful photos!
- 17 February 2008 at 17:47
- Nina's Kitchen (Nina Timm) said...
You definitively know the way to a Valentine's heart - lovely pictures too. My first visit and I will be back soon.
- 17 February 2008 at 19:45
- Alexx said...
Wow - they look delicious! And I love the photography on your blog.
- 17 February 2008 at 20:24
- Toni said...
How beautiful are these? Besides, I think shellfish counts as an aphrodesiac!
- 17 February 2008 at 22:52
- Dhanggit said...
omg the photos look absolutely delicious..i love crabs but in france its kinda hard to find really fresh than what i grew up with..most of the time they are frozen and im not really satisfied with the smell and taste..but your photos really gave me so much envy to eat some today :-)
btw, i tagged you on sharing about 5 random facts on yourself, thanks
- 18 February 2008 at 10:19
- Sylvie said...
Even if you didn't dress the crab yourself, you definitely presented them beautifully! Hope you had a good Valentine's!
- 18 February 2008 at 13:52
- Heather said...
I love that presentation! Crab shells are so pretty, I think.
- 18 February 2008 at 16:12
- Kate / Kajal said...
lol .. ! well these "crabs" look adorable.
- 19 February 2008 at 12:08
- Sophie said...
Very artistically presented!
We ended up having the pancakes we didn't get round to on Shrove Tuesday
- 20 February 2008 at 20:46
- Antonia said...
What gorgeous presentation! Just beautiful.
- 21 February 2008 at 18:44
- Browners said...
What a brilliant photo. Looks delicious.
- 26 February 2008 at 09:43
Many jewelers have personnel links jewellery on staff that is able to create a unique piece of jewelry to your specifications, links of london bracelet sale and if there are no personnel that can accomplish this task, links of london charms sale they can order it specially made for you. Not only do you have links of london womens watches the option of making this piece exactly the way you want it links of london rings sale to look, but no one will have one exactly like it. links of london necklaces sale Unique diamond jewelry does not have to be gaudy, but many people think gaudy is gorgeous.
- 16 August 2010 at 04:08
- wholesale clothing said...
You definitively know the way to a Valentine's heart - lovely pictures too. My first visit and I will be back soon. 2945abc45 0215
- 15 February 2011 at 08:28


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Carseat Canopy is one of the many creations made by a Pleasant Grove, Utah at-home mother named Jenny Pierce. It delivers baby products under the Mothers Lounge brand.
It's about a decade old already; a project that Pierce started along with her Milk Bands breastfeeding bracelet innovation, which was sold globally in thousands of stores. The Carseat Canopy brand and the carseat canopy coupon offerings they come along with to make them more affordable mostly deal with infant accessories inside the car, which includes blankets, seat covers, baby seats, and (of course) canopies.
The Different Types of Carseat Canopy Coupons
- Carseat Canopy Discount Coupons: This online incentive is quite handy because it allows you to get a wealth of quality canopies with a certain amount discounted from the item's total cost. For example, you might get a $10 Carseat Canopy discount coupon for a $19.99 purchase. That means you could acquire a Carseat Canopy brand canopy for $9.99 or more than half its price when you apply that coupon for that product. Keep in mind though that there are certain restrictions when it comes to these coupons that you need to observe, like the fact that if you're buying a car seat cover or blanket worth $10, you won't be able to use this coupon on it.
Ditto when it comes to certain deals accompanying your discount coupon, like you getting the $10 discount if and only if you could spend more than $25 of Carseat Canopy product, so instead of simply buying a canopy for $19.99, you need to add the $10 blanket in order to get the discount, or else you can't use the coupon. Even with that restriction, you're getting a great deal, because it's either you're getting half the price of a canopy or you're practically getting the blanket for free. At any rate, this is the most straightforward type of Carseat Canopy coupon you could get, if not the most common of them all.
- Carseat Canopy BOGO Coupon: If you've ever heard of "Buy One, Take One" deals, then this is the online coupon equivalent of it. BOGO in this instance stands for "Buy One, Get One" or "Buy One, Get One Free", which is essentially the same thing. It's another straightforward coupon type that should allow you to get good savings on various Carseat Canopy products but with understandable restrictions on what you're supposed to buy so that the merchant doesn't troubled by customers who are trying to pull one over them. For example, there's a limit to what sort of canopy and baby cover items from Carseat Canopy you could buy or get for free.
These restrictions are installed so that you won't end up buying a $10 blanket in order to get a $59.95 canopy for free. Of course, even with these limitations in place, there are many ways you can use these coupons to your advantage. For mothers who want to maximize their savings when using this coupon, the wise thing to do is is not use them for items you won't bother buying in the first place. Instead, concentrate on getting free items that you would've bought even if they're not for free. There are loads of motherly and babyish products you could choose from Carseat Canopy for purchase, particularly Udder Covers Seven baby slings or the Carseat Canopy Nursing Pillow.
- Carseat Canopy "Buy One, Get One Half-Off" Coupon: It works the same way as a BOGO coupon, but only this time your online coupon code gets you half off of certain items when you buy certain items. This is the type of deal Carseat Canopy would probably allow so that you can get that $59.95 canopy you want after just buying a $10 blanket or some sort of similarly priced nursing set; instead of getting the $60 canopy for free, maybe you could acquire it for half-price at $30 or $29.98. There's not as much savings with this coupon type compared to BOGO, but there's still savings present when you get a BOGO Half-Off instead of a BOGO Free.
What's more, you're likelier to get this special type of online discount coupon for the really expensive items that the store won't simply give away as a promotional incentive, like Udder Cover or Carseat Canopy sets. Again, the wise shopper will only use this coupon for important purchases that they want from the start. It's after all foolish to buy something you don't really need for the sake of savings. You should instead pay for something at half-price if you're willing to pay for it for full price anyway in order to truly get worthwhile savings off of the item, whether it's buying a single Carseat Canopy or a Whole Caboodle set.
- Carseat Canopy "Buy Several, Get One Free" Coupon: Carseat Canopy also offers this BSGO Free coupon deal wherein you can buy a certain set of items in a package deal of sorts, and the company will throw in a free item to increase your possible savings. This BOGO online coupon variant is a good deal if you're purchasing a staple item that you're going to use regularly or for the foreseeable future (like a free sling included in a whole carseat canopy set with nursing covering). Just watch out for delivery payments and whatnot that could undo whatever savings you've gotten from the set or package deal. Make sure to buy from the official site or a third-party site with free deliveries offered.
You could also check out if there are free deliveries done in your area. Remember, if you're buying something in bulk and you're getting a little something that's worthwhile as an extra, then this is a good deal. If the freebie is being used so that you can buy a set of supplies you wouldn't really need, then reconsider buying it or get a better online discount coupon that would truly give you worthwhile savings when you use it. Otherwise, you're simply wasting your money on the guise that you're saving money instead. With that said, Carseat Canopy does offer a lot of worthwhile bulk packages that most mothers approve of, more often than not.
- Carseat Canopy Coupons for Members: If you have a Carseat Canopy membership that you've availed of from the official site or if you've joined the site's mailing list, you're eligible to get privileges, incentives, deals, and coupons that aren't available a Google search away like all the other coupons in this list. This "members only" type of coupon allows you access to exclusives that non-members couldn't get, usually sent directly to your email in the form of a newsletter post and the like. You can even get certain other perks like loyalty rewards and even bigger discounts from coupons that aren't accessible to the rest of the public. Just be wary of impulse purchases or signing up for memberships that have fees.
This can result in huge savings if you play your cards right.Instead of getting small-scale freebies with no free delivery option included so you end up paying upwards of $12 for services that should cost $2 to $3 maximum, you'll get to avail of sneak peeks on samples of new products and designs, experimental fabrics, bigger percentages in discounts and vouchers (especially if you've been a member for a long, long time), and other coupon types you won't normally find from even the most dedicated of coupon repositories on the Worldwide Web. You could even get vouchers and gift codes every day if you're a loyal customer with regular purchases, like someone handling daycare for preschoolers and whatnot.
- Carseat Canopy "Get an Item Free with Each Purchase" Coupon: Not to be confused with BOGO coupons, this type of coupon offers you free items that aren't necessarily for sale when you make purchases online or at certain brick-and-mortar stores (as opposed to ecommerce ones). There are times whenever you see a coupon that offers a free item when you buy it over at a specific store or at a certain amount. In essence, you're getting something extra as long as you fulfill the stipulations of the coupon. For example, you might get a free sling, bib, or pacifier by spending $25 or more on Carseat Canopies at a local brick-and-mortar store or by Amazon.com order.
This is a great deal of a coupon as long as you can meet its conditions, which usually involves buying at a specific store or site (so it can be considered a store coupon, in a sense) and spending a set amount of money (or buying a set number of products as well as patronizing a package deal) on Carseat Canopy items, from canopies to blankets and baby seat covers. Just make sure that the free item you're getting is a worthwhile one that your baby can use. Otherwise, if you already have your fill of bibs and pacifiers, then don't bother buying a whole set of canopies just to get the advertised free item of something you don't need.
- Carseat Canopy "Get an Item Free" Coupon: As for this coupon, it's a rare type of coupon wherein you can get an item for free when you use it, no strings attached. No conditions, no stipulations, and no "get this item only when you buy this amount of products or if you buy several items in one go" deals. If you're wondering where the catch is with this incentive, then the catch is actually you. If a product is available for free, more often than not, it's the buyer that's "for sale" or the consumer that's the "product". This marketing ploy is quite effective in spreading the word about upcoming Carseat Canopy products that require testing and feedback from consumers.
You're not really getting something for nothing as though a charitable organization handed the Carseat Canopy freebie to you, although the company will typically present this as a loyalty reward or member coupon exclusive to Carseat Canopy mailing list members. One thing you should be wary about with freebies from this company or in general is getting an item you have no use for. They're also betting that once you get to the store or the sight in order to retrieve your "prize", you might decide to impulsively buy something while you're there, which is where their agents and upsell salespersons come in. In order to keep the clutter down in your home and get canopies you absolutely require, use this freebie coupon wisely.
- Carseat Canopy Free Shipping Coupon: As many bargain hunters would suggest, this is the coupon type you should stock up on, but make sure you have the date of their expiration before making a free shipping inventory of sorts for them so that you won't end up with expired and worthless coupon codes for Carseat Canopy products down the line. Usually, unless otherwise specified by the Carseat Canopy website or third-party ecommerce resellers like Amazon.com, you have to pay for some sort of delivery fee before receiving your baby canopy with various designs that include Angelina, Chevy, Hayden, Hunter, Jagger, Jayden, Kendra, Kennedy, Mikayla, Noa, Solomon, Tyler, Aiden, Belle, Eastwood, Hawkslee, Knott, Lovely, Maddox, Reagan, Sprinkled, and so forth.
That's just a given. It's therefore convenient to be able to have a way to get free shipping even by sites that don't do free shipping with the help of these coupons, especially if you live far away or even overseas yet want to avail of these items with shipping and handling already paid for by coupon deal. Next to discount coupons, free shipping coupons for Carseat Canopy and other products in general are the second most common coupon types you'll see available everywhere online. This ensures that you won't have to pay extra for shipping. You don't have to purchase a certain number of products in order for this free shipping coupon to work, at that.
- Carseat Canopy Rebate Coupons: A rebate, as opposed to a discount, provides you a good amount of savings but requires you to send the receipt and the coupon together to get the savings. Essentially, you pay for your products full price, get a rebate coupon, then present proof of purchase and the coupon to get part of your investment back. These Carseat Canopy coupons are particularly handy when it comes to purchases you've made that you can't use a coupon on since they've already been paid for. It's like a retroactive type of discount wherein you get cash in the end in the form of refunds. As you can imagine, there are loads of conditions to make this possible.
There are limits to what sort of products can be rebated, how recently they were purchased or else the rebate will be considered inapplicable, and so on and so forth. These coupons are especially and specifically beneficial when you're buying big ticket items like consumer electronics, but they have their own uses for low ticket items like baby car seat covers and canopies to protect your infant and keep him or her warm. They also only work if you're diligent in sending them as soon as you make your purchase because there's a deadline on how long ago the item was purchased before its rebate value "expires".
- Carseat Canopy Printable Coupons:Carseat Canopy isn't only available online through mail order with its official site and several ecommerce resellers. There are a number of baby supply stores that also sell this product. It therefore makes sense that it has printable coupons where there's a percentage off of a given product or total purchase cost at checkout. These discount coupons or any other type of coupon available on the list (like the BOGO coupon variants) can be printed out using your printer or through a printing shop then cut out so that they could be physically presented in a brick-and-mortar store. This is convenient for people who don't want to wait for mail order shipment to arrive.
That's definitely possible since more and more Carseat Canopy products, baby supplies, and design variants are available in stores nationwide. If you're the kind of person who wants to get your baby covers, blankets, and canopies immediately fresh off the box as soon as you purchase it, then you can get these products by offering printed out coupons to participating baby supplies shops across the country. You can get individual discounts on items, discounts on the final cost, a free item for every purchase, a free item after a collection of purchases, and almost everything except free shipping (since you're already at the store and getting free shipping for an item that's already there is redundant).
The many different Carseat Canopies for infants and mothers as well as the varied coupons you can use to get huge savings for them showcases Jenny Pierce's continued efforts to make every mom's life easier in everything that she does.
Jenny always wants to make a difference in mothers' lives everywhere, which is why all her sought-after baby products can be made more affordable through discount or rebate coupons as well as BOGO deals. Just pick and choose which online or offline coupon is most useful for you given your circumstances and you're good to go.


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Subscribe To Will Lex Luthor Be Back For Justice League Part One? Here's What Jesse Eisenberg Has To Say Updates
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After his debut as the villainous Lex Luthor in last weekend's Batman v Superman: Dawn of Justice, Jesse Eisenberg seems well set up as one of the DC Cinematic Universe's big bads. Though don't get too excited about the possibility of seeing Lex Luthor anytime soon, as his fate is apparently not as set in stone as we'd thought.
During an interview with Business Insider, Eisenberg was asked about whether the rather ominous ending of Batman v Superman: Dawn of Justice was hinting at his own continued involvement in the DC Comics film canon. His reply was the following, slightly surprising statement:
I don't know if they are so settled on where my character goes. I only hope I have another opportunity to work with these wonderful people and play this interesting character.
In a world where multi-film contracts are the norm, and multiple appearances are seeded throughout the entirety of a cinematic universe once a character is in play, the fact that Jesse Eisenberg's Lex Luthor isn't set for Justice League: Part One could be a slight cause for concern. While becoming a box office behemoth after this weekend's exemplary performance of Batman v Superman: Dawn of Justice, the Zack Snyder film has earned its fair share of detractors. Not only that, but some of those detractors have demanded that Snyder be removed from the director's chair for the first of two Justice League films planned for the current phase of DC Comics' film slate. Should Warner Bros. somehow decide to bow to the pressure, this could leave the door open to recast any roles that the new director may feel need to be reworked for the long run.
It's this possibility, coupled with Jesse Eisenberg's statement that Lex Luthor's future is up in the air, that has us wondering if any hypothetical new helmer for Justice League: Part One would take the opportunity to re-cast Metropolis' criminal mastermind with another actor. Though judging by the attention to continuity that the DC Cinematic Universe is trying to implement, as well as the character traits for this version of Lex Luthor being deeply rooted in Jesse Eisenberg's portrayal, it does not seem like a likely possibility. While the next appearance of Eisenberg's Luthor may not be set in stone, it doesn't seem like he'll be joining the ranks of Katie Holmes or Terrance Howard any time soon.
The DC Cinematic Universe is at an interesting crossroads at the moment, with Batman v Superman: Dawn of Justice managing to both break records and illicit the most varied audience response of any superhero movie in quite some time. Obviously any decisions that are to be made about Justice League: Part One will have to be weighed carefully, so as not to veer too closely to either the comic fans or movie fans following this franchise. You can take and compare notes with your fellow movie patrons as Batman v Superman: Dawn of Justice is in theaters now.


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Pope Francis admits that Hell exists
We have been lucky enough to be granted an interview with Pope Francis (yes, that Pope Francis). In fact we never actually met him, or spoke to him, but this is not considered necessary these days, and we have decided to make the whole thing up. Take it away, Francis!
"Yes, I know I allowed Scalfari to interview me - a 93-year-old lunatic who switches off his hearing aid when he interviews, doesn't take notes, and makes up his own account of what happened. And I know I've been fooled by him 489 times before. But we were told to forgive people 70 times 7 times, and this is the last," explained the Holy Father.Thank God for laughter. It keeps us sane in an insane world.
"And yes, Eccles, I do believe in Hell. Look, can you imagine something nastier than this?" [You'll have to go to the source to see the photos and the rest of the article.]


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