text:
The authors have no conflicts of interest.
Research Article
Transforming growth factor-alpha induces endothelin receptor A expression in osteoarthritis†
Version of Record online: 7 MAR 2012
DOI: 10.1002/jor.22099
Copyright © 2012 Orthopaedic Research Society
Journal of Orthopaedic Research
Volume 30, Issue 9, pages 1391–1397, September 2012
Additional Information
How to Cite
Usmani, S. E., Appleton, C. T. G. and Beier, F. (2012), Transforming growth factor-alpha induces endothelin receptor A expression in osteoarthritis. J. Orthop. Res., 30: 1391–1397. doi: 10.1002/jor.22099
- †
Publication History
- Issue online: 12 JUL 2012
- Version of Record online: 7 MAR 2012
- Manuscript Accepted: 15 FEB 2012
- Manuscript Received: 24 NOV 2011
- Abstract
- Article
- References
- Cited By
- osteoarthritis;
- articular cartilage;
- transforming growth factor alpha;
- endothelin receptor A;
- type II collagen, MMP-13
Previously, our lab identified transforming growth factor-alpha (TGFα) as a novel factor involved in osteoarthritis (OA) in a surgical model of the disease. In the same study, we also observed increased transcript levels for endothelin receptor A (ET(A)R), a known contributor to cartilage pathology. To investigate the connection between TGFα and endothelin signaling in OA, primary articular chondrocytes and osteochondral explants were isolated from Sprague–Dawley rats and treated with vehicle or TGFα. Expression of ET(A)R protein and its encoding gene Ednra was assessed. Chondrocytes and cartilage explants were also treated with the endothelin receptor A/B antagonist Bosentan, in order to determine whether TGFα effects could be blocked. TGFα induced expression of ET(A)R protein and its encoding gene Ednra. In primary chondrocyte cultures, Bosentan did not block TGFα responses of the anabolic genes Sox9, Agc1, and Col2a1, but reduced the induction of Mmp13 and Ednra transcripts by TGFα. In osteochondral explants, the inhibitor partially blocked TGFα reduction of type II collagen, as well as induction of MMP-13 and type II collagen neoepitopes. TGFα induces ET(A)R expression in articular chondrocytes and receptor antagonism appears to block some TGFα-induced catabolic effects in a three-dimensional organ culture system. Thus, TGFα may be a therapeutic target upstream of ET(A)R in OA. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1391–1397, 2012
Osteoarthritis (OA) is the most prevalent disabling condition in western society and places a serious strain on our healthcare system.1 Clinical symptoms such as joint pain and decreased joint function can severely affect day to day life for patients; however, no cure exists and current treatments only address symptoms without affecting the underlying causes.2 As a result, there is a significant need for further investigation and understanding of OA pathology.
While OA is a multivariable disease affecting the entire joint, one defining feature is the degeneration of articular cartilage.1 To better understand underlying pathological mechanisms, our lab has recently established a genome-wide profile of differentially expressed genes in degrading cartilage in a surgical rodent model of OA.3 Interestingly, transforming growth factor-alpha (TGFα) transcript levels were upregulated in degenerating cartilage when compared to controls.3 Thus TGFα was identified as a novel candidate growth factor involved in cartilage degradation. Further exploration revealed that TGFα treatment had profound effects on the chondrocyte phenotype. For example, treatment of monolayer chondrocyte cell cultures with TGFα resulted in suppressed expression of anabolic genes including aggrecan and type II collagen while matrix metalloproteinase 13 (MMP-13) gene expression increased.4 TGFα treatment also altered chondrocyte morphology and induced stress fiber formation in monolayer cultures.4 Articular cartilage organ culture studies revealed that cartilage treated with TGFα expressed more MMP-13 than controls and also displayed other OA-like characteristics such as cell clusters.4 A subset of human OA patients was also found to have higher than normal TGFα mRNA levels, thus establishing an important correlation between our rodent model and the human disease state.4
TGFα is a member of the epidermal growth factor (EGF) family and contains the characteristic EGF-like domain which binds to the EGF receptor.5 While EGF itself was downregulated in our experimental OA model, EGF receptor phosphorylation was increased, indicating receptor activation.4 Furthermore, a recent study showed that genetically modified mice with enhanced EGFR signaling spontaneously develop OA.6 EGFR signaling is also known to overlap and interact many other signaling systems, including the G-protein-coupled receptor (GPCR) endothelin receptor A (ET(A)R).7 In articular chondrocytes specifically, it has been observed that EGF induces expression of ET(A)R as well as of its ligand, endothelin-1 (ET-1).8, 9 Our interest in interactions between these two signaling systems emerged firstly from the fact that, like TGFα, ET(A)R gene expression was upregulated in our animal model of OA3 and secondly from the body of research implicating both systems in OA disease progression.
Previous studies have indicated that ET(A)R and its ligand play important roles in chondrocyte physiology and pathology. Studies using rodent articular chondrocytes showed that ET-1 production and ET(A)R density increased with age.9, 10 This trend is significant as age is the number one risk factor associated with OA.2 Prolonged exposure to ET-1 has also been shown to inhibit proteoglycan and collagen synthesis in rat articular chondrocytes.11 These data are complemented by reports which indicate that ET-1 increases expression of MMP-1 and MMP-13 in human osteoarthritic chondrocytes.12, 13 Human osteoarthritic chondrocytes also respond to ET-1 with increased expression of inducible nitric oxide synthase (iNOS), as well as elevated nitric oxide (NO) production.13 Elevated levels of iNOS are also characteristic in OA cartilage and studies have shown that inhibitors of iNOS can reduce the progression of experimental OA.14–16 Recently, Kaufman et al.,17 elegantly demonstrated that antagonism of ET(A)R prevents articular cartilage degradation in a surgical rat model of OA. In this study, we hypothesized that TGFα signals upstream of ET(A)R and that inhibiting ET(A)R might block some of TGFα's OA-like changes in articular cartilage.
Surgical Model of Osteoarthritis
Tissues from our original surgical OA model were analyzed in this study. Weight controlled adult male Sprague–Dawley rats underwent anterior cruciate ligament transection (ACL-T) and partial medial meniscectomy (PM) of the right knee joint or sham surgery as described.18, 19 All animals were exercised on a rotating cylinder for 30 min three times a week in order to allow full flexion and extension of their knee joints.18
Histology and Immunohistochemistry
Organ culture explants were fixed overnight in 4% paraformaldehyde and then decalcified in an EDTA solution (0.4 M EDTA, 0.3 N NaOH, 1.5% glycerol, and pH 7.3). Decalcification was determined by a physical end-point test. Tissues were processed, embedded in paraffin wax, and sectioned (6 µm thick) by the Molecular Pathology Laboratory at Robarts Research Institute (London, ON, Canada). Immunohistochemistry (IHC) was performed by first dewaxing sections in xylene and then rehydrating through a series of graded ethanols ending in water. Antigen retrieval was performed in sodium citrate pH 6.0 for cellular proteins or Proteinase K for matrix-specific proteins. Tissues were then blocked in 5% serum and incubated with primary antibody overnight at 4°C. Incubation with horseradish peroxidise-conjugated secondary antibody was followed by colorimetric detection with the substrate diaminobenzidine (DAB). Primary antibodies used include anti-ET(A)R, -ET(B)R, and -iNOS (Abcam, Cambridge, MA), anti-type II collagen (R&D Systems, Minneapolis, MN), anti-MMP-13 (Cedarland, Hornby, ON, Canada), and anti-type II collagen neoepitope.20, 21
Cell Culture and Organ Culture Studies
All cell and organ culture reagents were purchased from Invitrogen (Burlington, Ont., Canada) and Sigma (Oakville, Ont., Canada) while sterile plates were purchased from BD Falcon (Mississauga, ON, Canada). All cells and explants were maintained in a 37°C humidified incubator at 5% CO2 and medium was changed daily.
Primary Articular Chondrocyte Cell Culture
Primary articular chondrocytes were isolated from the distal femoral condyles of neonatal Sprague–Dawley rats as previously described and plated at a density of 5.0 × 104 cells/cm2.22 A 10% fetal bovine serum culture medium was prepared from a 2:3 ratio of DMEM:F12 supplemented with 50 µg/ml ascorbic acid, 0.25% L-glutamine, and 0.25% penicillin/streptomycin. Chondrocytes were serum starved for 24 h prior to treatment and serum-free medium was used throughout the remainder of the time course. Chondrocyte culture medium was supplemented with TGFα (final concentration 10 ng/ml) or an equal volume of vehicle (0.1% bovine serum albumin in PBS) for up to 72 h. Culture medium was changed daily. For our endothelin inhibitor studies, cells were treated with vehicle, TGFα (10 ng/ml), the endothelin receptor inhibitor Bosentan (10 µM; provided by Actelion Pharmaceuticals Ltd, Allschwil, Switzerland) or a combination of both for 48 h. Bosentan is a dual endothelin receptor A/B antagonist which is clinically used for the treatment of pulmonary arterial hypertension.23
E15.5 Tibia Oragan Culture
Time-mated CD1 mice were purchased from Charles River Laboratory (St. Constant, Quebec, Canada) and embryonic E15.5 tibia were dissected as previously described.24, 25 Tibiae were cultured in media containing α-MEM supplemented with ascorbic acid, beta-glycerophosphate, bovine serum albumin, PENSTREP®, and L-glutamine. All tissues were maintained in a 37°C humidified incubator at 5% CO2 and treated with a range of concentrations of recombinant human TGFα (0–1,000 ng/ml) for 6 days. Culture medium was changed every other day.
RNA Isolation and Real-Time PCR
RNA was isolated from rat primary chondrocytes using the Qiagen RNeasy Mini Kit and the manufacturer's animal cell protocol (Qiagen, Mississauga, ON, Canada). Mouse cartilage was dissected from the ends of tibia organ cultures with the aid of a Zeiss Stemi DV4 Stereo microscope as previously described,26 samples were placed in QIAzol solution (Qiagen), and RNA was isolated following the manufacturer's protocol. Real-time PCR was performed for the genes Ednra (endothelin receptor A), Il1b (interleukin-1 beta), Cxcr4 (chemokine (C-X-C motif) receptor 4), Tgfa (TGFα), Agc1 (aggrecan), Col2a1 (type II collagen), Sox9 (Sox9), and Mmp13 (MMP-13). Analysis was performed using the Applied Biosystems 7900HT Real-Time PCR system, the TaqMan® One-step Mastermix Kit, and commercially available probes (Applied Biosystems, Foster City, CA). All samples were normalized to the housekeeping gene Gapdh and day 0 or vehicle-treated controls using the delta-delta cycle threshold (ΔΔCt) method.
Articular Cartilage Organ Culture
Osteochondral explants were isolated from the distal femoral condyles and proximal tibial plateaus of 4- to 5-month-old adult male Sprague–Dawley rats. Explants were then placed in 12-well tissue culture plates and submerged in a 2% bovine serum albumin culture medium made from alpha-minimal essential medium supplemented with 50 µg/ml ascorbic acid, 0.25% L-glutamine, and 0.25% penicillin/streptomycin for 24 h prior to treatment. The same treatment groups described for primary chondrocyte experiments were repeated for organ culture studies. Explants were treated daily for up to 5 days before tissues were prepared for histology.
Rhodamine Phalloidin Staining
Primary chondrocyte cell cultures were isolated as described above and plated on micro cover glasses (VWR, Mississauga, ON, Canada) in 24-well plates at a density of 1.0 × 104 cells/cm2. Cells were treated with 1 of 4 treatments: vehicle, TGFα (10 ng/ml), Bosentan (10 µM), or both for 48 h. Cells were then fixed, permeabilized, and stained with rhodamine phalloidin (Cytoskeleton Inc., Denver, CO) to visualize F-actin and overall cell morphology.
All statistical analysis was performed using GraphPad Prism software Version 4.0. Real-time data were analyzed using either a one-way analysis of variance (ANOVA) with a Tukey's post-test or a two-way ANOVA with a Bonferroni's post-test. All graphs show mean values + standard error of the mean (SEM). A minimum of three independent groups was used in all experiments.
ET(A)R Expression Increases in a Surgical Model of Osteoarthritis
Our previous studies have shown that ET(A)R gene expression is upregulated in OA-operated animals compared to controls, as demonstrated by microarrays and validated by real-time PCR.3 We next wanted to examine ET(A)R protein expression in histological samples from OA- and sham-operated animals. Immunohistochemical analysis revealed more ET(A)R signal throughout articular surfaces in OA animals compared to controls (Fig. 1).
TGFα Treatment Induces Ednra Gene Expression
To examine whether TGFα can induce Ednra expression, primary rat chondrocytes in monolayer culture were treated with vehicle or TGFα (10 ng/ml) for 1–3 days, followed by isolation of RNA and real-time PCR analyses. Ednra mRNA expression increased after 2 and 3 days of TGFα treatment (Fig. 2A). Day 2 showed about a ninefold increase while day 3 showed about a sixfold increase (Fig. 2A). In contrast, transcript levels of several other genes known to be involved in OA or identified in our microarray, including Tgfa itself, Il1b, and Cxcr4, did not change significantly after treatment (Fig. 2B–D). We next wanted to determine whether TGFα-induction of Ednra was species specific. We therefore employed the mouse E15.5 tibia organ culture system. RNA isolated from the cartilage ends of TGFα-treated tibiae also showed an induction of Ednra transcripts, although a higher dose of TGFα was required to reach significance (Fig. 2E).
TGFα Treatment Induces ET(A)R Protein Expression
To study TGFα's effects on ET(A)R expression in a more physiological three-dimensional context, articular organ culture explants were isolated from male Sprague–Dawley rats and treated with or without TGFα. Tissues were immunostained for ET(A)R and ET(B)R and representative images from days 1 to 5 are shown (Fig. 3A). Figure 3 reveals an increase in ET(A)R staining in TGFα-treated explants compared to controls. No evident change in ET(B)R expression was observed between treatments and controls (Fig. 3A).
iNOS and NF-κB Expression Increase with TGFα Treatment
Endothelin signaling is known to activate iNOS expression.13 Tissue sections from articular cartilage organ cultures treated with vehicle or TGFa were stained with antibodies against iNOS and the p65 subunit of NF-κB (a transcription factor regulating iNOS expression27). The TGFα-treated tissues appear to have more positively stained cells for iNOS than controls after 5 days of treatment (Fig. 3B). In particular, staining extended deeper into the cartilage in TGFα-treated samples. We saw a similar trend for NF-κB staining (Fig. 3B).
Bosentan Treatment Does Not Block TGFα-Induced Gene Changes
To address whether inhibition of endothelin receptors can block effects of TGFα on gene expression, primary chondrocytes were treated with vehicle, TGFα, the endothelin receptor A/B inhibitor Bosentan, or both. Bosentan treatment did not block TGFα-suppression of Agc1, Col2a1, or Sox9 mRNA levels (Fig. 4A–C); however, Bosentan slightly decreased the induction of Ednra (Fig. 4D) and Mmp13 (Fig. 4E). In the absence of TGFα, Bosentan did not induce any significant changes in chondrocyte gene expression.
Bosentan Treatment Does Not Block TGFα Effects on Cell Morphology
TGFα has been shown to alter cell shape and induce stress fiber formation in monolayer articular chondrocyte cultures,4 in agreement with loss of a chondrocyte phenotype.28 As in our earlier studies, TGFα induced stress fiber formation and a more elongated cell shape in primary chondrocytes (Fig. 5). By itself, Bosentan did not appear to have any effects on cell morphology or actin organization (Fig. 5). Moreover, when Bosentan treatment was given in combination with TGFα, no major change was observed when compared to TGFα treatment alone (Fig. 5).
Bosentan Treatment Blocks TGFα Effects on Cartilage ECM Turnover
After finding limited effects of endothelin receptor A/B inhibition in our monolayer articular chondrocyte model, we decided to perform inhibitor studies in the more physiologically relevant organ culture model. Explants were cultured with vehicle, TGFα, Bosentan, or both. Tissue sections were then immunostained for type II collagen, MMP-13, and type II collagen neoepitopes that become detectable when type II collagen is cleaved by MMP-13. TGFα-treated tissues showed a decrease in overall type II collagen staining in the extracellular matrix (ECM; Fig. 6A) and an increase in MMP-13 staining (Fig. 6B). Co-treatment with Bosentan appeared to reverse these catabolic effects (Fig. 6A and B). Furthermore, TGFα-treatment appeared to generate more type II collagen neoepitopes in the ECM when compared to controls, but the combination with Bosentan appeared to block neoepitope induction (Fig. 6C).
We have previously identified TGFα as a growth factor capable of inducing OA-like phenotypic changes in articular chondrocytes. In this study, we demonstrate that TGFα induces ET(A)R expression in articular chondrocytes at both the gene and protein level. Furthermore, we demonstrate that TGFα-induction of the receptor occurs in both rat and mouse models as well as in varying ages of tissue (embryonic, newborn, and 4- to 5-month old). This is consistent with previous findings, in which EGF was able to induce ET(A)R expression in both young and old rats.9 In addition to EGF and TGFα, a number of other growth factors and cytokines are known to modulate ET(A)R receptor density in chondrocytes.9 Furthermore, the literature suggests that the ET(A)R receptor plays a role in both aging and degenerative joint disease.9, 10, 12, 13 Thus, TGFα induction of ET(A)R might be one way through which TGFα mediates its deleterious effects.
To test this hypothesis, we used the dual endothelin receptor A/B inhibitor Bosentan in combination with TGFα treatment. We found that Bosentan treatment was not sufficient to block TGFα-effects on anabolic gene expression in monolayer culture. Bosentan did, however, have a minor effect on Mmp13 and Ednra levels. Bosentan was also unable to reverse TGFα-induced changes to the chondrocyte actin cytoskeleton and cell shape. This leads us to believe that other downstream pathways might be responsible for anabolic gene expression changes and cytoskeletal rearrangement in TGFα-treated chondrocytes. Previous studies done in our laboratory show that TGFα activates many intracellular signaling pathways.21 For example, RhoA/ROCK mediates TGFα-induced morphologic changes in chondrocytes, in agreement with our previous studies demonstrating important roles of this pathway in chondrogenesis.29, 30 The MEK/ERK pathway mediates the downregulation of anabolic gene expression by TGFα,21 again in line with the known roles of this pathway in chondrocytes (reviewed in Refs.31, 32). Both Rho/ROCK and MEK/ERK pathways also regulate type II collagen cleavage and aggrecan breakdown in articular cartilage.21
Bosentan had a stronger effect in the three-dimensional organ culture system where endothelin receptor A/B inhibition was able to suppress TGFα induction of the catabolic factor MMP-13 and subsequent type II collagen break down. There are some possible explanations for the differences observed in our two experimental systems. Firstly, age may play a role: primary cells used in our studies were isolated from neonatal rats while organ culture explants were isolated from adult rats. The baseline density of ET(A)R receptors is known to be dependent on the age of articular chondrocytes and more ET-1 is known to be produced in older chondrocytes.9, 10 In addition, endothelin receptor signaling might be more important in the authentic three-dimensional context of cartilage than in monolayer culture. Recently, Kaufman et al.17 showed that ET(A)R inhibition prevented OA progression in a surgical model of the disease, in agreement with our data shown here.
In our studies, we observed no effect on any parameters with Bosentan treatment alone. While articular chondrocytes produce ET-1, it is possible that its levels are not high enough in the in vitro environment to sufficiently stimulate ET(A)R receptors. ET-1 mRNA levels however, did not change in the TGFα treatment groups (data not shown), suggesting that it is the receptor density itself more so than the concentration of ligand that is responsible for enhanced signaling.
In summary, our data show that ET(A)R expression in articular cartilage is increased in response to both surgical induction of OA and TGFα treatment. We also demonstrate that this pathway is only partially responsible for TGFα-induction of an OA-like phenotype, but that ET(A)R inhibition suppresses catabolic activities in articular cartilage. Further studies should be done to examine the potential benefit of upstream targets such at the EGF receptor itself as well as combinations of downstream targets in OA therapy.
We would like to thank Dr. Jason Rockel (Toronto, ON) for teaching and assisting with the primary chondrocyte isolation protocol. We would also like to thank Vasek Pitelka (London, ON) for his assistance with the osteochondral explant isolation. We are grateful to Actelion Pharmaceuticals Ltd. for the donation of Bosentan as well as to Dr. Robin Poole (Montreal, QC) for donating the type II collagen cleavage antibodies. This work was funded by an operating grant from the Canadian Institutes of Health Research (CIHR, MOP86574). S.E.U. was supported by a CIHR Doctoral Award, a Graduate Scholarship from the Canadian Arthritis Network and an Ontario Graduate Scholarship in Science and Technology. F.B. is the recipient of a Canada Research Chair.
- 12006. Osteoarthritis: pathobiology-targets and ways for therapeutic intervention. Adv Drug Deliv Rev 58: 128–149., , , et al.
- 22006. Osteoarthritis. Adv Drug Deliv Rev 58: 150–167.,
- 32007. Global analyses of gene expression in early experimental osteoarthritis. Arthritis Rheum 56: 1854–1868., , , et al.
- 42007. Transforming growth factor alpha suppression of articular chondrocyte phenotype and Sox9 expression in a rat model of osteoarthritis. Arthritis Rheum 56: 3693–3705., , , et al.
- 51995. Transforming growth factor alpha. Cell Biol Int 19: 373–388., ,
- 62005. Targeted disruption of Mig-6 in the mouse genome leads to early onset degenerative joint disease. Proc Natl Acad Sci USA 102: 11740–11745., , , et al.
- 71999. Epidermal growth factor receptors: critical mediators of multiple receptor pathways. Curr Opin Cell Biol 11: 184–189., , , et al.
- 82000. Endothelin-1 in monolayer cultures of articular chondrocytes from young and old rats: regulation by growth factors and cytokines. Mech Ageing Dev 114: 37–48., , , et al.
- 92001. Endothelin-1 receptors on cultured rat articular chondrocytes: regulation by age, growth factors, and cytokines, and effect on cAMP production. Mech Ageing Dev 122: 519–531., , , et al.
- 102007. Articular chondrocyte aging and endothelin-1. Cytokine 37: 6–13., , , et al.
- 112002. Mechanism of inhibition of endothelin-l stimulated proteoglycan and collagen synthesis in rat articular chondrocytes. Cytokine 17: 254–261., , , et al.
- 122003. Endothelin 1 promotes osteoarthritic cartilage degradation via matrix metalloprotease 1 and matrix metalloprotease 13 induction. Arthritis Rheum 48: 2855–2864., , , et al.
- 132005. Endothelin-1 in osteoarthritic chondrocytes triggers nitric oxide production and upregulates collagenase production. Arthritis Res Ther 7: R324–R332., , , et al.
- 142000. Selective inhibition of inducible nitric oxide synthase reduces progression of experimental osteoarthritis in vivo: possible link with the reduction in chondrocyte apoptosis and caspase 3 level. Arthritis Rheum 43: 1290–1299., , , et al.
- 151995. The expression and regulation of nitric oxide synthase in human osteoarthritis-affected chondrocytes: evidence for up-regulated neuronal nitric oxide synthase. J Exp Med 182: 2097–2102., , , et al.
- 162006. Nitrite and nitrotyrosine concentrations in articular cartilage, subchondral bone, and trabecular bone of normal juvenile, normal adult, and osteoarthritic adult equine metacarpophalangeal joints. J Rheumatol 33: 1662–1667., , , et al.
- 172011. Nociceptive tolerance is improved by bradykinin receptor B1 antagonism and joint morphology is protected by both endothelin type A and bradykinin receptor B1 antagonism in a surgical model of osteoarthritis. Arthritis Res Ther 13: R76., , , et al.
- 182007. Forced mobilization accelerates pathogenesis: characterization of a preclinical surgical model of osteoarthritis. Arthritis Res Ther 9: R13., , , et al.
- 192006. Characterization of articular cartilage and subchondral bone changes in the rat anterior cruciate ligament transection and meniscectomized models of osteoarthritis. Bone 38: 234–243., , , et al.
- 201997. Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage. J Clin Invest 99: 1534–1545., , , et al.
- 212010. Rho/ROCK and MEK/ERK activation by transforming growth factor-alpha induces articular cartilage degradation. Lab Invest 90: 20–30., , , et al.
- 222003. TNF alpha suppresses link protein and type II collagen expression in chondrocytes: Role of MEKl/2 and NF-kappa B signaling pathways. J Cell Physiol 197: 356–369.,
- 232010. Bosentan. Expert Opin Pharmacother 11: 1023–1034.,
- 242007. C-type natriuretic peptide regulates endochondral bone growth through p38 MAP kinase-dependent and -independent pathways. BMC Dev Biol 7: 18., , , et al.
- 252008. The PI3K pathway regulates endochondral bone growth through control of hypertrophic chondrocyte differentiation. BMC Dev Biol 8: 40., , , et al.
- 262007. Genetic ablation of Racl in cartilage results in chondrodysplasia. Dev Biol 306: 612–623., , , et al.
- 27Regulation of the expression ofinducible nitric oxide synthase. Nitric Oxide 23: 75–93., , , et al.
- 282007. Regulation of chondrocyte differentiation by the actin cytoskeleton and adhesive interactions. J Cell Physiol 213: 1–8., ,
- 292004. RhoA/ROCKsignaling suppresses hypertrophic chondrocyte differentiation. J Biol Chem 279: 13205–13214., , , et al.
- 302006. RhoA/ROCK signaling regulates chondrogenesis in a context-dependent manner. J Biol Chem 281: 13134–13140.,
- 312003. MAP kinases in chondrocyte differentiation. Dev Biol 263: 165–175., ,
- 322010. Biology and pathology of Rho GTPase, PI-3 kinase-Akt, and MAP kinase signaling pathways in chondrocytes. J Cell Biochem 110: 573–580.,


text:
Why? Because you can! And pickle, and jam, or otherwise celebrate the resurgence of the domestic arts our forebears held so dear. Put on your best apron and step into our kitchen, won't you?
Ever yearn for berry jellies during the middle of winter? Look no further, using organic fruit juice plus a few other staple ingredients you can make a berry'licious jelly anytime of the year!
Sweet, vinegar fennel pickle. Try this pickle in lieu of traditional relish on any sandwich or burger, mix it into salads, or use it as a cocktail garnish.
Adult friendly cinnamon whiskey jelly made with Bulleit bourbon, cinnamon sticks, cider, sugar, pectin, lemon, and love. A sweet cinnamon jam with hints of whiskey notes...
This high alcohol Imperial IPA gives this mustard a pungent ale aftertaste. Hoppy and grainy this condiment would be great served atop a pretzel or bratwurst.
These pickled pears are more sweet than pickle'y. A great sweet treat that can be enjoyed alone, with a pastry type dessert, or over ice cream.
A quick and simple french jardiniere using an assortment of cauliflower, peppers, and carrots. Great side dish or appetizer.
A savory pear chutney myriad of pears, shallots, ginger, raisins, almonds, etc. Well suited as a snack dip, with cheese, or atop a roasted pork butt, other meat, or vegetarian main dish.
Hot kickin' beets offer a spicy alternative to the traditional sweet pickled beets. A water-bath canned pickled beet recipe made with whole peppercorns, garlic, thyme, hot peppers, cider vinegar, salt, and of course beets!
Easy-to-make homemade sriracha salt
A traditional cinnamon-infused rum, perfect for gifting or drinking yourself!

subset: commoncrawl


text:
Current projects
The Creator (2017)
Role: Alan Turing
Official Site. Photos. IMDb
Enemy of Man (2017)
Role: Banquo
The Snowman (2017)
Role: TBA
Release: Oct. 13 2017
Gernika (2016)
Role: Henry Howell
Release: 26 April 2016
Agent Carter (TV Series 2016, S.2)
Role: Edwin Jarvis
Release: 19.01.2016
Survivor (2015)
Role: Paul Anderson
Release: May 2015
Jupiter Ascending (2015)
Role: Maximilian Jones
Release: 06.02.2015
Agent Carter (TV Series 2015, S.1)
Role: Edwin Jarvis
Release: 06.01.2015
Broadchurch (TV Series 2015, S.2)
Role: Lee Ashworth
Release: 05.01.2015
In development
Section 47 (feature film)
Role: attached
Raising Finance
Official Site. Photos. IMDb
JamesD'Arcy.net is a non-profit fan website and is not affiliated with James D'Arcy or his representatives.
Please read disclaimer & terms of use here.
Latest updates
- 1
- 2
- 3
Interview with James D'Arcy by Jake Freels
28-07-2016 Hits:59
Jake Freels, was able to interview James who was on the set via e-mail through his agent. The young man didn't meet with James so we have no photo. The interview was posted on his Future Filmakers Move Reviews Blogspot and his mom, Jessica brought it to our attention through her twitter @greenflagfly. Many thanks!!! Wednesday, July 27, 2016 AN INTERVIEW WITH ACTOR JAMES D'ARCY I was fortunate to interview actor James D'Arcy of...Read more
Latest advert: BMW Vision #Next 100
02-07-2016 Hits:161
BMW celebrated their centenary with the unveiling of their futuristic vision car for the next 100 years, focusing on innovative technologies to meet the driver's every need. This car is self-driving! Published on June 6, 2016 on BMW UKRead more
New set of Guernica stills
08-06-2016 Hits:269
Sony Pictures Germany just released a gorgeous set of stills for Guernica. Most of them are of James! Enjoy!!!Read more
First Set Photos of James D'Arcy with Kenneth Branagh
05-06-2016 Hits:384
On Friday, 03 June 2016, the 10th day of shooting Christopher Nolan's Dunkirk, we finally have our first glimpse of James! He appeared with Kenneth Branagh on a rainy and wind-swept Malo East Pier where most of the filming took place since the beginning of the week...on the pier with explosions and on ships. Nolan and his cinematographer, Hoyte van Hoytema (Interstellar / Tinker, Tailor, Soldier, Spy) filmed their scene on the...Read more
Gernika at Huesca Film Festival and will be in cinemas on July 22
05-06-2016 Hits:192
On 21 June, 2016 (Tuesday), Gernika will be presented at the 44th Huesca International Film Festival ahead of its commercial release. The new theatre release date is now 22 July, 2016 (IMDB) It was previously slated for 09 September. Details of the schedule for Gernika at Huesca (English translation) below:Read more
Voiceover for Queens Park Ranger FC - New Crest Launch
25-05-2016 Hits:219
Three weeks ago, James had time to do voiceovers for QPR Football Club's official launch of their new crest. After a 6 month consultation with Rangers supporters through social media, a new symbol was chosen and unveiled just before the 2016/17 season start at Loftus Road on 06 May 2016. And the end-result was that the crowd was well-pleased. Two exciting videos voiced by James accompanied the official reveal. They tell of the...Read more
James D'Arcy Joins Christopher Nolan's war film, Dunkirk
24-05-2016 Hits:400
We can now guess why James had this super-short haircut last week, posted by Tess Ward on twitter (May 17th). After Guernica, James seems to have attached himself to another gripping and powerful war story. Batman Begins director, Christopher Nolan began production on his World War II epic action thriller "Dunkirk", on location in the historical coastal town on Monday, 23 May 2016. From the Warner Bros, press release, we have a...Read more
New "Guernica" Trailer and DVD/Digital Release Date
24-05-2016 Hits:231
The Playlist released a new exclusive trailer for "Guernica," And today we have the exclusive trailer debut for the movie that details an important chapter in WWII history. Starring James D'Arcy, Jack Davenport, María Valverde, Burn Gorman, and Ingrid García-Jonsson, the film brings brings audiences to Spain in 1937, where the citizens of the village of Guernica live with the day-to-day realities of the Civil War. An American journalist trying to report on what's...Read more
BBC Radio Trail for The Hollow Crown
02-05-2016 Hits:362
This week, Phoebe Fox and James D'Arcy are on the BBC Network Radio Trail for The Hollow Crown on Radio 3 & 4. On HQ Voice on SoundCloudRead more
Gorgeous "Gernika" Poster for Malaga
21-04-2016 Hits:357
Koldo Serra's new movie poster for Gernika which is competing for the Golden Biznaga at the Malaga Film Festival was unveiled today and it is stunning! Gernika opens next Tuesday: To Purchase Tickets 26 April 2016 Teatro Cervantes: Press Pass at 12.00 and 22.00 27 April 2016 Cine Albeniz: 19.00 and 23.15 @gernikathemovie informs us that the premiere will be attended by Koldo Serra, the producers, as well as Maria Valverde, Ingrid Garcia-Jonsson...Read more
Focusing on BMW 7 series - 4 new adverts
21-04-2016 Hits:311
Last week, 4 of James' voiceovers were added to BMW UK youtube. Busy man! 11 April 2016: BMW 7 SeriesRead more
"Chicken and Egg" - a short film Directed by James D'Arcy
20-04-2016 Hits:558
Over the weekend in London and wrapping on Monday, it seems James was very busy directing a short film. (17-18 April, 2016) Back in March 2016, we first noticed James' name in a young actor's CV for "Chicken and Egg" as a Director. We thought no more of it since James was still filming The Snowman in Norway. After Snowman wrapped at the end of March, James was seen dashing out of...Read more
Gernika Teaser Trailer
13-04-2016 Hits:288
The Gernika Teaser Trailer is finally here! Gernika - Teaser Trailer Abril 2016 VOSE from Pecado Films on Vimeo and on eitbkultura Gernika, The Movie. Con James D'Arcy (Cloud Atlas), María Valverde (Exodous), Jack Davenport (Piratas del Caribe). Sección Oficial Festival de Malaga, proyección el 26 de abril de 2016, Aniversario del Bombardeo. 04/13/2016 The following is the exclusive on El Mundo El Mundo exclusively presents the first images of Gernika , the film Koldo Serra...Read more
James D'Arcy Presents the revolutionary BMW XDrive Baby Boots
07-04-2016 Hits:381
On April 1, BMW UK launched their new xDrive baby boots, which react to a toddler's unbalanced movements in a fraction of a second to provide more secure toddling around corners and total stability in all conditions. Great idea, no? When you visit the BMW showroom, they have a range of lifestyle accessories such as shirts, jackets, travel luggage, and key chains. But then, we know what an incredibly creative prankster James...Read more
"Gernika" Competes at Malaga Film Fest, "The Snowman" Wraps & Other News
30-03-2016 Hits:389
"Gernika" is in the Official Selection of the 19th Malaga Film Festival which takes place from 22 April to 01 May 2016. The Malaga Festival press announced today that "Gernika" by Koldo Serra and the black comedy, "Quatretondeta" by Pol Rodriguez have been selected for the official competition for the Golden Biznaga, along with 12 other titles which were announced earlier. "Gernika" debut screening is on Tuesday, 26 April 2016 at the...Read more
Excerpts of Interviews about James D'Arcy
06-03-2016 Hits:522
With the airing of the Finale this week, Hayley Atwell and the showrunners made their round of interviews to wrap up Season 2 of Marvel's Agent Carter. Here are excerpts from their interviews offering revelations of what a national treasure James D'Arcy is! (well, we already knew that..) On finding out that there was going to be a musical number It was hilarious! First of all, James [D'Arcy] jumped up like a schoolgirl...Read more
BBC Radio: "Yours Sincerely" and BMW TV ad
03-03-2016 Hits:376
This coming Sunday, 06 March 2016, James will do a reading on BBC RADIO 3. Words and Music: Yours Sincerely Rosalie Craig and James D'Arcy read extracts on the theme of letters. BBC Radio 3: Sun 6 Mar 2016 at 17.30 Duration: 1 hour, 15 minutes Letters can be the most intimate form of expression, defiantly public, or a window onto somebody's life at a particular time. They can be funny or tragic, or in...Read more
Agent Carter's Dreamy Musical Number Tonight!
23-02-2016 Hits:385
"Daniel, what in the bloody blue blazes is happening," Agent Peggy Carter asks. In tonight's episode of Marvel's Agent Carter, Sousa (Enver Gjokaj) drops his crutches and starts dancing and singing out Peggy's inner thoughts during dream, bringing along friends and foes, Angie (fan-favorite Lyndsey Fonseca), Dottie, Whitney, Wilkes, Thompson, Rose, Ana, and of course, Jarvis! Peggy's dream sequence was choreographed by Dancing with the Stars, Louis van Amstel and includes DWTS...Read more
Smoke and Mirrors - "Agent Carter" Ep.4 promos
31-01-2016 Hits:309
"Agent Carter" Episode 4 Smoke and Mirrors will air next Tuesday Febr 2 on ABC. Synopsis: Agent Carter and the SSR learn there's more than just a pretty face behind Hollywood star Whitney Frost, Peggy's most dangerous foe yet. Here are the trailer and a funny sneak peek of episode 4. Stephen Patterson from Movie Pilot wrote this excellent review after the British premiere of Agent Carter S2: I was skeptical, after completing...Read more
Agent Carter "Better Angels" Promos and Re-broadcasts
24-01-2016 Hits:306
Photo credit: Kelsey McNeal / Marvel / Disney ABC TV Group. More pics in our gallery. Ep 3 "Better Angels" of Marvel's Agent Carter airs on ABC, this TUESDAY, JANUARY 26 (9:00-10:00 p.m. EST) This episode was written by Jose Molina and directed by David Platt. Peggy uncovers secrets and lies about the bizarre murder case. Her search for the truth about Zero Matter will put her on a collision...Read more
James D'Arcy confirmed in "The Snowman" filming from January to March in Norway
22-01-2016 Hits:1004
James has been confirmed to have a role in "The Snowman" with Michael Fassbender and Charlotte Gainsbourg. James was seen at the Steen Mall & Strom in the Oslo city center trying out clothes for the film. Filming began on Monday, 18 January 2016 at Frogner Park and in Oslo and will continue until the end of March. Today, the film's press officer confirmed with NRK that James is one of the actors of...Read more
Agent Carter S.2 premiere tonight on ABC
19-01-2016 Hits:322
Tonight, January 19, 9 PM EST on ABC we will see the first two episodes of Agent Carter Season 2: The Lady in the Lake and A View in the Dark. ABC released several clips from the new episodes: Hypable.com has a funny behind the scenes footage that previews an interview with James and Hayley.Read more
Premiere Photos for Agent Carter Season 2
09-01-2016 Hits:465
Marvel released the the Premiere Photos for the first 2 episodes of Agent Carter season 2. It shows wonderful pictures of Jarvis going to the races, chasing Flamingos, lifting weights, fencing and wrestling... and generally, just looking incredibly #jarvalicous!!! Jose Molina tweeted "Y'all should've seen the outtakes of this. Poor James... #AgentCarter" We wonder if James caught the flamingos or if they chased him... Jarvis is working out!!! Will he use...Read more
Sky Safari: Australia
07-01-2016 Hits:470
This one seems to have sneaked under everyone's radar. The docuwiki says James hosted a nature documentary that was aired by National Geographic Wild in 2015. "Join an aerial odyssey across Australia's iconic landmarks of Uluru, the Great Barrier Reef, and Lake Eyre as we reveal how these unique environments affect the creatures who call them home." Sky Safari: Australia 1x1 hour (NHNZ co-production with Nat Geo Wild) An aerial odyssey across a...Read more
James D'Arcy Talks Agent Carter Season 2 & Jarvis Character Portrait
06-01-2016 Hits:531
ABC Press released a bunch of interviews with the cast and crew of Agent Carter today. James D'Arcy discusses what Season Two means for Jarvis... Hayley Atwell (Agent Peggy Carter) Chad Michael Murray, Enver Gjokaj, newcomers Wynn Everett & Reggie Austin, Head of Marvel Television Jeph Loeb, and the Agent Carter showrunners Michelle Fazekas, Tara Butters and Chris Dingess also talk season two. Their interviews can be viewed on the MCU Exchange on...Read more
Agent Carter Extended Synopsis and New Year Promo
02-01-2016 Hits:435
Another action-packed promo aired on New Year's Eve as we counted down to midnight. "This new year, be bold, be bad and dress to kill" was the theme. With only a couple weeks to go till the Agent Carter Season 2 premiere, ABC released an extended synopsis so we know that Peggy and Jarvis will have quite a lot on their plates on their next adventure in Hollywood. "Marvel's Agent Carter" returns for...Read more
First Gernika Stills
31-12-2015 Hits:593
We are pleased to report that the the post-production work on Gernika will be completed in January 2016 and the film will be ready to premiere in March 2016. The ADR was completed and the recording of soundtrack by renowned Spanish composer, Fernando Velazquez was done in Budapest. From the Pecado Films website, 12 stills have been posted! They look wonderful! And from the Gernika IMDB, 3 more new ones.. ...Read more
Agent Carter Season 2 Wrap, Dancing with the Stars, ShaBoom and BMW 7 Silhouette
28-12-2015 Hits:453
The filming of Season 2 of Agent Carter officially wrapped on 19 December 2015. Agents of Shield had a wrap party, but it seems Agent Carter cast didn't have one. Geoffrey Colo posted the fanmail address for both Agent Carter and Agents of Shield: (same address) Entertainment Weekly reported that Angie Martinelli would make an appearance in a dream sequence which would cross over into an epic song and dance number. This should make Lyndsy Fonseca...Read more
Agent Carter first preview, new BMW X1 TV advert and filming in the desert
08-12-2015 Hits:577
In this new preview, Jarvis cries to Peggy the hilarious reasons why he finds California life unbearable and how his life now consist of "oiling floorboards, cleaning pools and transporting exotic livestock" and begs to work for her again. A new UK TV Advert on embracing the unknown with the new BMW X1. And a great photo from producer Louis D'Esposito "Hanging out with the boys" from the set of Agent...Read more
Season 2 Agent Carter US and UK premieres delayed 2 weeks
03-12-2015 Hits:390
Because of President Obama's State of the Union address to both Houses of Congress on 12 January, the launch dates for season 2 of Marvel's Agent Carter has been delayed. Most likely, the networks did not want to air a new season with an interruption. To recap, they were originally slated to start on Jan. 5th (US) and Jan. 17th (UK). The new launch dates are now: (with a 2-hour debut, after...Read more
"The Next Big Thing" Agent Carter promo #4 and a new BMW 7 advert
30-11-2015 Hits:436
Our first glimpse of Mrs. Jarvis (Lotte Verbeek) and James in a wrestling singlet in the Agent Carter promo and a new BMW 7 Series Advert highlighting the innovation of comfort.Read more
James D'Arcy takes his seat to support his drama school
29-11-2015 Hits:843
James joined LAMDA alumni, Jim Broadbent, Benedict Cumberbatch and Dominic Cooper in backing a fundraising campaign to complete their drama school's £28 million redevelopment campaign. In the Act Now! Take Your Seat campaign, donors can name a seat at LAMDA's new 200-seat Simon Sainsbury Theatre for a £500, £1,000 or £2,000 donation. The money raised would go towards the remaining £3 million needed for building the new theatre, an education wing and...Read more
"Peggy Carter is Back" Agent Carter Season 2 Promo #3
26-11-2015 Hits:393
A third promo "Peggy Carter is Back" but she seems to be having a few surprises with Howard's car.Read more
Awards for Children on the Frontline
26-11-2015 Hits:480
Last year, James narrated a documentary for ITN Productions. It was 48.29 long and was released on Channel 4 on Jan 22 2014. The documentary also aired on Canal, ARD, ZDF, CNN and Al Jazeera. Children on the Frontline was very risky to make and took great courage from its filmmakers, Marcel Mettelsiefen and Anthony Wonke. It showed a portrait of the Syrian conflict from the eyes of 5 young children...Read more
Tractor Ted Down at the River
24-11-2015 Hits:413
During early October, the latest DVD, Tractor Ted Down at the River was launched with premiere screenings in 20 Cinemas across UK, from Southampton to Edinburgh (19 Picturehouse Cinemas and Eclipse Cinema in Donegal). Tractor Ted Down at the River was screened as part of Picturehouse's Toddler Time. In this 17th DVD, UK's favourite little green tractor takes, voiced by James D'Arcy, takes us on an adventure to show us many...Read more
Latest BMW UK TV Advert and 7 Series "Innovation" Ads
24-11-2015 Hits:781
Catching up with James D'Arcy is a bit like catching the wind.. Here are some more of his most recent voiceovers on BMW 7 series Innovation ads that were published on BMW UK youtube. He must have done some of these ADRs from Los Angeles where he is currently busy filming Agent Carter Season 2. The BMW 7 Series features 24 new innovations such as gesture control. 3D camera control, bespoke...Read more
"New Adventure" Agent Carter S2 TV Spot #2
23-11-2015 Hits:446
A second TV Promo was aired during the American Music Awards tonight. In case you missed it, here it is again. It's Jarvalicious!!!Read more
First TV Spot for Agent Carter Season 2
18-11-2015 Hits:420
ABC aired the first promo for Marvel's Agent Carter 2 season during last night's episode of Agents of Shield. It looks like Peggy and Jarvis are making the trip to Hollywood for more lights, action, humour, romance and adventure! A new suave scientist is on the promo, Jason Wilkes (Reggie Austin) hinting of a new romance for Miss Carter. New characters include Vernon Masters (Kurtwood Smith), a veteran of the War...Read more
Gernika's final cut! More pranks... For Halloween?
30-10-2015 Hits:471
Gernika is finished! From Jose Manuel Jimenez Martinez Facebook: It's a wrap! After many months of work we have completed the montage of "Guernica" by Koldo Serra. Very happy with the experience and very happy with the result. Do not miss it !! Coming soon to cinemas ... It seems James has a lot of time on the set :) Hayley tweeted today: Walked into my trailer and found this ungodly site. Thanks...Read more
Isn't he amazing?
30-10-2015 Hits:558
The photo above is a screen capture from the new "darcing" video (see the previous post), but this short video deserves the post of it;s own. James reciting a poem of Gerard Manley Hopkins (1844-1889), The Leaden Echo, on set of "Agent Carter". Amazing! #jarvellous pic.twitter.com/z1AqNiMAfb — Hayley Atwell (@HayleyAtwell) October 15, 2015 THE LEADEN ECHO How to kéep—is there ány any, is there none such, nowhere known some, bow or brooch or braid...Read more
Filming "Agent Carter" 2
30-10-2015 Hits:459
New faces, new pranks on "Agent Carter" set in Los Angeles. From Marvel.com: At New York Comic Con, Marvel Television announced several new members to the cast of Marvel's Agent Carter including Wynn Everett, Lotte Verbeek, Currie Graham and Reggie Austin! Wynn Everett ("The Newsroom," "Charlie Wilson's War") will play Whitney Frost, a Hollywood actress and brilliant inventor. In the comics, Whitney Frost first appeared in TALES OF SUSPENSE #98 in 1968...Read more
Team Carter won Dubsmash Wars for Charity
30-10-2015 Hits:427
Congratulations Team Carter for winning the Dubsmash Wars! Hayley Atley and James D'Arcy raised $79 259 for Stomp Out Bullying. Team Shield came close raising $ 75 070 for St. Jude Children's Research Hospital. Here are James and Hayley in Step 2 and Step 3 of the contest: Team Shield was winning until the very last day when Hayley posted this short video on Twitter: "We dare you to post yourself doing this...Read more
Catching up with James D'Arcy
08-09-2015 Hits:955
After attending the San Diego Comic-Con in July, James flew back to London briefly. Not one to sit still for long, James did a reading of a new play for Emily Dobbs Productions, with Freddie Fox, Nicholas Farrell and Jessie Buckley. The play is in development stage. James also did 3 new BMW adverts for BMW UK and a Tractor Ted clip. That's what he does in his spare moments between...Read more
James loved his Birthday Jar!!
03-09-2015 Hits:533
When Jennifer gave the birthday jar to Hayley Atwell at the LA Comic Book and Scifi Con, Hayley said James would go mental. Here's James at the Agent Carter Season 2 cast read through with his birthday jar. Hayley tweeted "He loves his Birthday Jar!" Hooray!!! Many, many thanks to Hayley!!! James celebrated his birthday by treating himself to a Sparkys... a non-fat sugarless frozen yogurt would be our guess for...Read more
James' Birthday is coming!
06-08-2015 Hits:678
We want to celebrate James' Birthday by giving him a Wish Jar. Read the info on the poster below: Show your love and send a wish!Read more
Dubsmash Deathmatch "Round 3"
28-07-2015 Hits:629
We thought it was all over when #TeamAgentCarter Hayley and James recruited Captain America, Chris Evans in the last dubsmash, "Another One Bites the Dust" which was followed by a "dirty dancing" celebration. According to clara-oswin-oswald on tumblr who was at London Comic-Con, hayley talked about how competitive she and james d'arcy got and how they were devastated when the shield cast did the video with clark gregg dressed as peggy...Read more
Hayley Atwell and James D'Arcy loving Agent Carter 'Brit fest'
26-07-2015 Hits:611
Published on the Irish Examiner today and all over UK News, along with a new video interview by Press Association... Hayley Atwell and James D'Arcy admit they are loving leading a British invasion of US TV in Agent Carter. The two young Brits play Peggy Carter and butler Jarvis in the Marvel TV spin-off series, along with Mamma Mia star Dominic Cooper as millionaire Howard Stark. James said: "It's a Brit fest! Hayley's character...Read more
Podcast and highlights of the SDCC2015 Press Room
25-07-2015 Hits:591
From AgentCarter.net by Director E there is announcement that they're putting together a podcast from San Diego Comic Con with interviews with Hayley Atwell and James D'Arcy and the producers of Agent Carter. Stay tuned as it will be released this weekend and added to this update. Meanwhile these are the highlights from the SDCC press room regarding Season 2: ·Agent Carter will be reassigned to Los Angeles. Jeph Loeb explains the...Read more
James' Best Things in Life on Red
23-07-2015 Hits:835
Just out on Redonline, James talks about his Best Things in Life. Red Magazine / Talking Point / by Frances Wasem / posted on July 22, 2015 What's your idea of a great night out? Dinner with family and friends is hard to beat. Your favourite thing to listen to? The new Blur album. *Magic Whip* Your most beloved book? Any Human Heart by William Boyd. Best television show? Anything starring either of my girlfriends...Read more
Dubsmash War Update: The mic has been dropped and thrown out of the window with the help of Captain America! And Hydra lost...
15-07-2015 Hits:1092
Over the weekend at the San Diego Comic-Con, we had the cast of Marvel's two shows, Agent Carter and Agents of Shield, engaged in a fierce battle of the greatest dubsmash battle in the history of social media. Florence, has compiled all the dubsmashing clips into the Agent Carter vs. Agents of Shield Dubsmash War playlist here. A quick browse should quickly bring you up to date on this hilarious...Read more
Video interviews with James and Hayley from San Diego Comic Con
14-07-2015 Hits:667
James and Hayley gave several video interviews during the San Diego Comic Con., some of them funny, some sweet, all of them interesting :) Tonya Papanikolas interviewed them for Spoiler TV. She wrote: But before you watch the video, here's what we learned from the executive producing team of Tara Butters, Michele Fazekas and Chris Dingess. First of all, the show is moving to Los Angeles for season two as Peggy is called...Read more
Nerd HQ 2015: A conversation with Marvel
13-07-2015 Hits:695
This photo above was made by Jennifer Carey from The Geek Girl Project, who attended the panel. Thank you! The Conversations for a Cause panel series from San Diego Comic-Con featured discussions with genre film and TV legends like Joss Whedon, Guillermo del Toro, Scott Bakula, and many more. It began on Thursday in The New Children's Museum in San Diego, but -for us - the most interersting panel took place...Read more
IGN interview and more photos from San Diego Comic Con
12-07-2015 Hits:627
IGN's Roth Cornet interviewed James on IGN Live from SDCC yesterday. We are going to see Jarvis's wife in season 2! James and Hayley attended SiriusXM's Entertainment Weekly Radio Channel Broadcasts too. Many new fan photos appeared - you can see them on our Pinterest and Facebook pages. The new stage of the dubsmash war began with Chloe Bennet's video. Here is James' reaction: This is what defeat looks like... #JamesD'Arcy @HayleyAtwell #AgentsofSHIELD #SDCC...Read more
James at the Marvel San Diego Comic Con booth signing
11-07-2015 Hits:726
Some lucky fans got to talk to James, like this girl from Philippines: Thank you James D'Arcy & @HayleyAtwell for being so kind -girl who took a 14-hr flight fr the Philippines to see you. pic.twitter.com/GqxkXHBcpC — Mima Mendoza (@ironmima) July 11, 2015 James, Hayley and Jeph Loeb were on Marvel SDCC live Marvel CC events you can watch live here. Update: the dubsmash war continues :) The killer blow. Thank you @ChloeBennet4 BOOM @clarkgregg https://t.co/SilK383hFf...Read more
Hayley and James at the San Diego Comic Con Day 2 - Dubsmash War
11-07-2015 Hits:504
It was a great day for Hayley and James and for Agent Carter team. We had much fun too :) Agents of S.H.I.E.L.D's actors Clark Gregg and Chloe Bennet called james and Hayley out for a dubsmash war. Here is the result: pic.twitter.com/PbAIBv58Cv — Hayley Atwell (@HayleyAtwell) July 11, 2015 You're on @ChloeBennet4 @clarkgregg pic.twitter.com/Dm41ZFwzSu — Hayley Atwell (@HayleyAtwell) July 11, 2015 Thank you @taylorswift13 and screaming goat @ChloeBennet4 @clarkgregg pic.twitter.com/fLizLJchKq — Hayley Atwell (@HayleyAtwell) July...Read more
Hayley and James at the San Diego Comic Con Day 2
10-07-2015 Hits:890
James getting ready for the Marvel TV Panel today. (From ABC Publicity on twitter) Day 2 of the San Diego Comic Con, began at 9 am with a sweet Peg and Jarv reunion followed by a SDCC breakfast. James said "Doesn't it feel like it's been a really long time!" In Ballroom 20, a large & excited audience had already assembled & waited for 3 hours. Shortly after 3 pm, the Agent...Read more
Avocado fries and an actor's life
09-07-2015 Hits:884
Red UK magazine, August 2015. "The other Mr. D'Arcy" Viv Groskop unearths Broadchurch baddie James D'Arcy's softer side. (pp. 78-81) I was allowed a screenshot from my digital subscription but you can purchase a single issue of the magazine in digital from Zinio or in print from Red UK. Click on the magnifying glass for a more readable size on our magazine scans gallery.Read more
James in one of the panels of "Conversations for a Cause"
03-07-2015 Hits:642
IGN TO STREAM NERD HQ'S CONVERSATIONS FOR A CAUSE FROM COMIC-CON (by Anthony Couto, UK.IGN.com) IGN is teaming up with Zachary Levi and The Nerd Machine's Nerd HQ to bring you a livestream of the Conversations for a Cause panel series from San Diego Comic-Con, featuring discussions with genre film and TV legends like Joss Whedon, Guillermo del Toro, Scott Bakula (Enterprise fans, rejoice!), and many more. The Conversations for a Cause...Read more
Peggy & Jarvis goes to Hollywood, and Jarvis hates it there!
01-07-2015 Hits:823
EW provided us with a first look of a new setting and backstory for Jarvis and Peggy in Season 2 of Marvel's Agent Carter, and a new comic-con poster!! (Image Credit: Kris Anka / Marvel/ABC) Agent Carter is heading to Hollywood! When the second season of the ABC drama kicks off, Peggy Carter (Hayley Atwell) will be westward bound thanks to the SSR. "There's a case that goes beyond what the LAPD...Read more
Entertainment Weekly Report
30-06-2015 Hits:549
(Kelsey McNeal/ABC) Marvel's Agent Carter: James D'Arcy, Enver Gjokaj retuning for season 2 Posted June 30 2015 — 5:30 PM EDT Entertainment Weekly / BY NATALIE ABRAMS • @NATALIEABRAMS When Peggy Carter heads out west, she won't be alone. EW has learned exclusively that James D'Arcy and Enver Gjokaj—who play Edwin Jarvis and Agent Daniel Sousa, respectively—have signed on as series regulars for season 2. While Marvel declined to comment, we hear deals for Chad Michael...Read more
Jarvis Joins Carter at the 2015 San Diego Comic-Con
As Marvel's Agent Carter will be returning for a second season2 on ABC, Peggy Carter (Hayley Atwell) and faithful sidekick, Edwin Jarvis (James D'Arcy) are heading off to the 2015…
That's a wrap for Gernika!
Photo from @GernikaTheMovie on twitter Filming for Gernika ended today, Thursday June 25, 2015, making it 8 weeks from when it began on May 3rd. From Monday to Wednesday of this week…
Week 7/8 for "Gernika" - Lekeitio, Gernika-Lumo, Gordexola
A little more than a week is left to complete the filming of "Gernika." On Monday and Tuesday (June 15 -16), Koldo Serra took his production crew of 60, a…
Instruments of Darkness
Vincent Regan released "Instruments of Darkness" to Kickstarter backers of Enemy of Man on 9th May 2015. It is 8.48 minutes long with the actors using the language of Shakespeare…
Beer, Biscuits and Bombs
Quite a lot has happened on the set of Gernika this week and all to confirm that James is indeed a bonafide certifiable sweetie!! On Friday night (June 5), La Salve hosted a…
Messages from fans
Hunter: Hello, James! I thought you'd be fun to draw so I drew you. I hope you like it. Can't wait to see Broadchurch S3! Eurydice_P: Just tell him he's a wonderful actor…
For you, fans! Gernika photos
Aritz Atela posted this photo on Twitter: Me and James D'Arcy. This one is for you, fans! Thank you very much Aritz! This photo from Gernika set is from Aritz' site: James doesn't…
James meets the President of the Basque Government
June 3, 2015 Source: Irekia Lehendakari Iñigo Urkullu has approached this afternoon Munoa Palace in the town of Barakaldo Bizkaia, where he is shooting the film "Gernika" directed by Koldo Serra. Gernika is a…
Stills from the set of Gernika
Beautiful stills from Gernika's Facebook page (photos by David Herranz): and from the photographer Aritz Atela's site Check out our gallery for more from the set pictures and our Gernika YouTube channel .
Gernika: Recreating "The Shining" at Arab Hall
Yesterday's filming of Gernika took place at the splendid baroque Arab Hall at the Bilbao City Council. The scene was that of a Basque High Society dance party. After the…
Filming Gernika - new photos
We've got several new pictures of James filming Gernica in Bilbao. Carlos Clavijo posted this one: Check out the video on our Instagram that shows that scene: Rolling in the centre…
Tribute to each generation of the BMW 3 Series
To celebrate the 40th anniversary of the beloved BMW 3 Series, FCB Inferno produced two online films that could be seen across social channels and on BMW UK Youtube, published…
Photos from the set of Gernica
This photo of James as Henry Howell we have to thank Hayley Atwell for - or maybe James himself, because he sent it to Hayley :) Very nice, isn't it? The…
Agent Carter season 2 news
Kelsey MacNeal on ew.com: After snagging a second season renewal, Agent Carter is leaving New York City behind. In the wake of saving the world once again and letting go of her…
Tractor Ted Meets Baby Animals
New Tractor Ted DVD is out: James is voicing Tractor Ted, of course! Tractor Ted takes on a tour of the farm with Fudge the dog who is full of excitement…
James confirmed to star in "Gernika" with Maria Valverde and Jack Davenport
News from Fotogramas.es 02/05/2015 EXCLUSIVE: JAMES D'ARCY AND JACK DAVENPORT ACCOMPANY MARÍA VALVERDE 'GERNIKA' Confirmed the international cast of the film. 'Guernica', which tells a love story between journalists in times of war…
2 new BMW adverts
Released last week, two new BMW adverts voiced by James. How wonderfully rich, smooth and silky is his voice! ❤❤❤ High Beam Assist on the new BMW 1 Series. Published on 15…
Sort of good news, sort of bad news for Enemy of Man
James D'Arcy filming trailer at Hedingham Castle in March 2013 It's hard to believe that more than two years have past and we're still waiting for the filming of Enemy of…
Broadchurch 2 Bonus Scenes
We have some great behind the scenes clips from Broadchurch Season 2 recently added to our jamesdarcynet youtube channel; many thanks to Alexander Bendo who have diligently saved. cut and compiled…
New BMW Adverts - BMW 1 Series
Found on BMW UK youtube channel, Published on March 23, 2015, a new advert for BMW, James voicing the narration. BMW 1 Series with Predictive Gear Shift Technology The new BMW…
Survivor: First Trailer
James D'Arcy as Agent Paul Anderson examining bomb fragments at a terrorist bombing site in London The first trailer of Survivor is out!!! In the fast-paced action trailer, Milla Jovovich is…
Random Pics
In lieu of news, our dear Florence Reidy found some random pics of James we might not have seen before. From instagram, many months ago: From Susan McGrath's facebook Sweet James, still taking…
Five EPK Interviews with James D'Arcy from Agent Carter
From Disney ABC Press, we have 5 epk interviews with James released on Feb. 26, 2015 but we have only recently discovered. 10. James D'Arcy, "Edwin Jarvis", on his character 11. James…
"Dot the I" - UK premiere on London Live
"Dot the I" is the pick of the day in the London Evening Standard. Its U.K. premiere airs at 10 pm tonight on London Live.
Jane Featherstone talks about casting James as Lee Ashworth in Broadchurch 2
Executive producer Jane Featherstone spoke to Mandi Bierly at Yahoo TV about the casting of James D'Arcy as Lee Ashworth, Hardy's main suspect in the unsolved Sandbrook case. This just…
Agent Carter wins 3 Saturn Awards Nominations!!!
The Academy of Science Fiction, Fantasy and Horror Films announced the nominations for this year's 41st Annual Saturn Awards and Agent Carter received 3 nominations!! Streaming live from Hollywood at Stream…
BBC America: Introducing 'Broadchurch' Season Two's New Cast Members
By Brigid Brown Posted on March 4th, 2015 - BBC America/Anglophenia The wait is over. David Tennant and Olivia Colman make their return to Broadchurch in the season two premiere on BBC AMERICA tonight (March 4)…
Broadchurch Season 2 airs on BBC America this Wednesday night and related news
Season 2 of Broadchurch will finally have its U.S. premiere on BBC America, this Wednesday on March 4 at 10 pm ET. James appears quite a bit on their teaser trailer…
MTV's 11 Hottest New Guys of the 2015 TV Season - James D'Arcy
To celebrate the age-old pleasure of watching super hot dudes on television, MTV chose the 11 hottest breakout dudes of the 2015 TV Season. We're assuming MTV hasn't seen James…
Hayley Atwell & James D'Arcy Speed Read for First Book
Do you recall a photo of James and Hayley Atwell reading to children as they were waiting to speak onstage at the 2015 Winter TCA press tour at the Langham Hotel…
- 1
- 2
- 3


text:
Tuesdays & Thursdays 1pm ET » Read More
Investors should forget about European stocks and look to emerging markets instead, says Peter Boockvar of The Lindsey Group.
India could be a key catalyst for oil prices, according to oil expert Helima Croft of RBC Capital Markets.
Dennis Gartman of The Gartman Letter discusses why he's buying into the cotton market right now.
They say that good things come to those who wait — and that couldn't be truer for the bulls on Wall Street, according to one technical analyst.
Barclays tells CNBC why the firm is bearish on oil for the next six to eight months, amid a large stockpile that could mean trouble.
Trader Todd Colvin explains why zero interest rates and a top-heavy market will continue to drive gold higher.
Ed Yardeni discusses concern for slowing global growth, but still remains positive on U.S. markets.
Peter Boockvar, chief market analyst at The Lindsey Group, voices his concerns as investors continue to flock to the bond market.
One of the stock market's biggest bulls spots a rare trend which could send stocks skyrocketing through 2017.
Ralph Acampora says the Brexit created a historic head fake within U.S. markets. He explains why he's extremely bullish on equities.
Former U.S. Rep. Ron Paul says the markets are "running out of steam," and a huge move lower is coming.
Tom Kloza of Oil Price Information Service sees a steady summer for crude, but a possible downturn in the fall.
Here's NorthmanTrader.com founder Sven Henrich's call for the S&P 500.
JPMorgan Private Bank's Stephen Parker says the "sell in May and go away" theory is a flawed concept this summer.
Zev Spiro, CEO of Orips Research, makes his case for why gold will reach $1,450.
If Great Britain leaves the EU, Wells Fargo says U.S. investors should buy stocks and not get spooked by volatility.
Peter Boockvar says investors should focus on a Brexit, not the Fed, as the key catalyst for global financial worries.
There's an 80 percent chance the stock market will go up in the next month because of a rare technical trend, according to FBN Securities.
Gold, crude, stocks and bonds are rallying in lockstep, and that has created a very tricky trading environment, according to one market watcher.
The market is miscalculating when the Fed will hike interest rates, according to Matarin Capital hedge fund manager Nili Gilbert.
CME Group brings buyers and sellers together through its CME Globex electronic trading platform and trading facilities in New York and Chicago.
Take your trading to the next level with a platform that lets you trade stocks, options, futures and forex all in one place with no platform or data with no trade minimums. Open an account with TD Ameritrade and get up to $600 cash.


text:
Type: Posts; User: smerf
Search: Search took 0.01 seconds.
June 3rd, 2015, 05:37 PM
Thread: DS3 -Sebastian or Theodoreby smerf
- Replies
- 12
- Views
- 824
DS3 -Sebastian or Theodore
We are still trying to decide on a name for DS3, due in a few weeks. We like Sebastian (Seb) and Theodore (Theo). Siblings are Felix and Elias (Eli). Do you prefer Sebastian or Theodore? Any other...
May 30th, 2015, 07:58 AM
- Replies
- 3
- Views
- 489
Baby boy no. 3 - Theo to go with Felix and Elias?
Hi there,
We need help naming DS3, due in a few weeks. Siblings are Felix and Elias (always called Eli). Theo is the only name we agree on but as we thought about using it for the first two also, I...
October 25th, 2012, 10:43 PM
Thread: second thoughtsby smerf
- Replies
- 3
- Views
- 711
I have two boys, Felix and Elias, so obviously I...
I have two boys, Felix and Elias, so obviously I love the name Elias! I had second thoughts about names during both pregnancies as well. We didn't really have alternative names we agreed on so went...
October 15th, 2012, 05:36 PM
Thread: Sibling name for brother Felixby smerf
- Replies
- 7
- Views
- 3,867
We have a 23 month old Felix, and his 6 month old...
We have a 23 month old Felix, and his 6 month old brother is Elias (only ever called Eli). I love Elias and felt that it was as classic and handsome as Felix but it is a bit of a mouthful hence Eli....
March 30th, 2012, 11:09 PM
- Replies
- 6
- Views
- 1,061
Thanks guys, your comments make me feel much more...
Thanks guys, your comments make me feel much more confident about our choice! :)
March 30th, 2012, 05:53 AM
- Replies
- 6
- Views
- 1,061
Thoughts on Elias, brother for Felix?
Hi there,
I am due to have our second baby next month and am hoping for some last minute advice. Our first sons name is Felix, with my maiden name as his middle name. My husband and I love the boys...
Results 1 to 6 of 6


text:
Tag Archives | DIY videos
Bag of Tricks: How to Make a DIY Bike Pouch
If our Pedal Pretty DIY series left you itching for more bike-based craft tutorials, consider this your lucky day! We've teamed up with Gwen Lutz of Nooworks to give you the skinny on how to make a handy handlebar pouch for your two-wheeler. Read more »
An Easy Peasy, Pretty DIY: Paint Chip Magnets
Bring out your true colors as an artist with this super simple DIY paint chip magnet project! Dedicate a few minutes or an afternoon to this low-commitment craft, and then get ready for a world of color. Read more »
Wear Your Heart on Your Knees
For me, Valentine's Day always brings me back to my grade school days, when we would sit at our desks, making valentines with construction paper and glitter. In the spirit of those days, I decided to do a little …Read more »
How to Twist and Tie Your Favorite Headscarves
If you've ever admired the effortlessly cool elegance of a perfectly tucked turban, or the retro flair of a youthful bow headband, check out our collection of informative DIY videos! Blog writers Erin and Annie set out to create a lighthearted guide which demonstrates just how fun–and easy–it is to achieve some of today's hottest accessory styles. Read more »


text:
Handcuffing Relievers: Vinnie Pestano and Chris Perez
Blame Mike Shanahan.
Thanks to the former Broncos coach, fantasy football players have had to come to grips with the reality that just because you draft a team's presumptive starter, it doesn't mean you'll get the team's production. Fantasy baseball players have had to work around platoon situations, but the idea of a handcuff is relatively new to this branch of the fantasy tree. For position players, traditional platoons are still a more likely issue that owners have to navigate, but especially for relievers, it's time to learn how to use a handcuff and which players are the most likely to need one.
For those unfamiliar with the term, handcuffs are players who may not have the name recognition of another player on their team, but who may share a role and split the available stats. For football, they are typically running backs who will share carries with a more well-known back, but in baseball, they're going to manifest themselves in a slightly different way. Rather than a job-sharing situation, handcuffs are going to be most useful for relievers who aren't secure in their role at the end of games.
Jeff Zimmerman covered Chris Perez's oblique issues on Monday, and while the Indians expect Perez to be back for the start of the season, some oblique strains can linger. Even if Perez is healthy in a month, there's a decent chance he won't be ready for high-leverage appearances until after the real games have started. In the meantime, Vinnie Pestano should grab whatever save chances are available as the team waits for Perez to get back into form. If this were a simple issue of Perez missing a little time, then coming back as the no-doubt closer, relegating the clearly inferior Pestano to a setup role, I'd actually advise against picking up Pestano for the short time he'd be valuable. That is not the current case. While Perez may believe the spot will be waiting for him when he returns, Pestano could yet render him a modern Wally Pipp.
Perez gave owners a solid 36 saves last year, ably doing the job he was drafted for, but providing little in the way of secondary value. Perez's 1.21 WHIP gave him the 10th highest WHIP of any pitcher with 20 or more saves and his ERA of 3.32 was the 7th worst. He's a flyball-heavy pitcher, who walks a fairly high number of hitters — just a hair under 4.0 BB/9 — and doesn't mitigate that with a lot of strikeouts, just 5.88 K/9 last season. Perez isn't bad, at least not Kevin Gregg bad, but he does allow a lot of balls in play and that isn't always the best trait for a high-leverage reliever to have.
Pestano, on the other hand, allows much less contact. He struck out more than a third of the hitters he faced last year and while he shares some of the same walk issues that Perez has, he allows opponents to hit a paltry .184 against him. His WHIP was a strong 1.05 and his ERA a solid 2.32 over the course of 68 appearances last year. In leagues were Holds are a category, Pestano is already must-draft and deeper leagues with specified RP slots may want to look his way as a solid source of strikeouts without too much risk in the WHIP and ERA categories. The question is whether he'll be able to grab a season or even half-season's worth of saves by unseating Perez.
I see two things working in Perez's favor as far as his ability to keep the closer's job. First, while he wasn't a strict closer in college, he has been used almost exclusively at the end of games when he's been at an age-appropriate level in the minors. He sat behind Kerry Wood in the majors in 2010, but when he was in the minors, he was the frequent choice at the end of games. History isn't fate, but when a player gets the "proven closer" label, it does make him harder to unseat. Second, 2011 was a remarkably worse year for Perez than 2010 was, and the Indians may be willing to see if he can regain that form, knowing Pestano will still be there in 2013 if Perez stumbles again in 2012. It's worth noting that 2011 Pestano was still better than 2010 Perez, but Manny Acta and his staff may see value in knowing which version of Perez is closer to reality.
Even knowing that Pestano faces an uphill battle, I still see him being draftable in all but the shallowest leagues, especially as a handcuff for Perez. If Perez's oblique keeps him out for more than the current 4-6 week timetable, owners will be glad they have Pestano to fill in the gap. If Perez gets his job back and then pitches his way out of it with another middling season, Pestano will again be the one who steps into the ninth inning. While holding on to Pestano in a traditional 5X5 set-up isn't ideal since he won't win or save many games, he'll still be an asset in three categories, which makes him a risk worth having on the roster.
He's being drafted in just 6 percent of mixed 5X5 leagues according to Mock Draft Central, which makes some sense, but he is certainly the type of late-round gamble that will almost certainly pay off whether or not he's getting saves in May and beyond. Yahoo! has him getting drafted 22 percent of the time, but with an ADP of about 221. Anyone planning to take Perez should be grabbing Pestano 100 percent of the time, but at that ADP, anyone with an open P or RP slot should be looking hard at Pestano. He's a safer pick than Joba Chamberlain and is going to generate more value — even in 5X5 — than teammate Grady Sizemore, both of whom have ADPs close to Pestano's.
The Bullpen Mafia is fertile ground for good relievers, so good in fact, that the best play on draft day is to break out the handcuffs and grab a pair of them.
Print This Post


text:
Not too long ago, driving an SUV was seen as an environmental offence, but the times are changing.
The Mitsubishi Outlander PHEV (it stands for Plug-in Hybrid Electric Vehicle) is a full-size five-seater SUV with 143mpg fuel economy and 49g/km emissions, plus it will qualify for the Government's £5,000 electric car grant. So is it the 'game-changer' the company claims it is?
We tried one of Mitsubishi's test mules, which hides the new hybrid drivetrain – as well as the PHEV's dash – under the body of an old Outlander.
The car features an electric motor on each axle, powered by a lithium-ion battery pack, to give four-wheel drive. It claims an electric-only range of up to 34 miles, but when necessary, a 2.0-litre four-cylinder petrol engine kicks in to drive the front wheels and charge the batteries.
Plug the Outlander into a regular power socket and you'll have the full EV range after four hours. And a quick-charging set-up provides 80 per cent battery capacity after just 30 minutes. Of course, you can always count on the petrol engine if you don't have access to a socket.
On the road, there's instant torque, so the car never feels too heavy or sluggish. Unlike some other hybrids, the petrol engine refuses to kick in below 62mph unless you floor the throttle, so this is a genuine zero-emissions commuter car. When it does fire up, you hear the engine over-rev as it picks up where the electric motors left off, but otherwise it's refined.
Showroom versions of the Outlander PHEV will package the batteries under the floor, so it's fair to say the lower centre of gravity will mean better handling than our test mule, which had the batteries in the boot. Yet accurate steering and good body control still bode well for the final model. If Mitsubishi can keep the price down, the PHEV looks like winner.
For an alternative review of the latest Mitsubishi Outlander 4x4 visit our sister site carbuyer.co.uk


text:
by Zack Beauchamp Jan 30, 2014
This company hawks marked up stuff that likely doesn't work - and made millions selling it to conservatives. The true story of how they took over the right.
by Ian Millhiser & Zack Beauchamp Jan 24, 2014
If Utah can cut of water or electricity to the NSA, what's to prevent Texas from cutting off power to federal agencies that provide health care to poor people?
by Zack Beauchamp Jan 21, 2014
The Wall Street Journal's Stephen Moore is a depressingly perfect fit for a think tank that acts less like a research group and more like a lobby.
by Zack Beauchamp Jan 17, 2014
Whether you're on President Obama's side in the surveillance debate or not, his speech didn't resolve the core issue Edward Snowden raised.
by Zack Beauchamp Jan 16, 2014
New researches exposes the way that the criminal justice system is insidiously undermining black Americans' clout in the political system.
by Zack Beauchamp Jan 13, 2014
A new report says there's not much evidence that your metadata is having a big impact in helping the NSA catch terrorists.
by Zack Beauchamp Jan 12, 2014
And the IRS. And Obamacare. And...
by Zack Beauchamp Jan 10, 2014
If the Republican Party is going to change, there needs to be a movement full of people who want to change it. Where is it?
by Zack Beauchamp Jan 9, 2014
The science that explains why so many sociopaths end up in politics.
by Zack Beauchamp Jan 8, 2014
What if it's not marijuana that's helping terrorists commit murder, but rather illegal marijuana?


text:
Reports & Results from Sunday's big club games
Keep up to date with all of the latest scores from today's provincial senior club games.
Keep up to date with all of the latest scores from today's provincial senior club games.
Limerick champions Na Piarsaigh have qualified for their third Munster club hurling final in five years after a 2-15 to 0-17 win over Thurles Sarsfields at the Gaelic Grounds.
Thurles Sarsfields showed the grit required to win championships as they retained the Tipperary Senior Hurling Championship final with a one-point win over Nenagh Eire Og at Semple Stadium this afternoon.
Podge Collins admitted he is living out a dream scenario with Cratloe as they advanced to the Munster Club SHC final after beating Thurles Sarsfields 1-18 to 0-14 on Sunday.
Pre-match favourites Thurles Sarsfields were dumped out of the Munster Senior Club Hurling Championship by Cratloe, 1-18 to 0-14.
Thurles Sarsfields are Tipperary senior hurling champions for a 33rd time after a 2-22 to 3-11 victory over last year's winners and mid-divisional rivals Loughmore-Castleiney.
Pat McAuliffe reports on De La Salle's victory over Thurles Sarsfields in the AIB Munster Club SHC final.
Newtownshandrum are through to the AIB Munster Club Hurling semi-finals after winning 0-19 to 1-15 against Thurles Sarsfields at Semple Stadium.
Tullamore claimed their first Offaly title in 45 years, while Thurles Sarsfields ran out easy winners in Tipperary.
Ger 'Redser' O'Grady has been nominated by county champions Thurles Sarsfields to captain the Tipperary senior hurlers for the coming season.
Tipperary will be without Lar Corbett for two months after the Thurles Sarsfields player fractured his ankle.


text:
I'm trying to transform a rather long sequence of values to a different format, using emacs' replace-regex command. There are more than 9 values to extract, so the \1 - \9 back references are not enough.
How can I back reference more than 9 capture groups in emacs? Can named groups be used? If so, how?


text:
Smiley and West's Poverty Tour: A call for unconditional brotherhood
Tavis Smiley and Cornel West brought the last stop of their "Poverty Tour: A Call to Conscience" to Memphis, Tennessee. The location was chosen in recognition of Martin Luther King, Jr.'s support for sanitation workers and as the site of the National Civil Rights Museum.
New federal immigration policy a step forward, supporters say
Last week the Obama administration announced it would suspend deportation proceeding against thousands of undocumented immigrants that pose no serious threat to national security or public safety.
In Connecticut the fight for jobs is on
"Connecticut workers want Congress to act on the jobs crisis with the same urgency that was brought to the manufactured political crisis over the debt ceiling."
Congreswoman introduces emergency jobs bill
Rep. Jan Schakowsky, D-Ill., plans to introduce the Emergency Jobs to Restore the American Dream Act, which will put over 2 million people to work.
Demand for job creation swells across the nation
At rallies, meetings, marches, demonstrations and press conferences from one end of the nation to the other demands for massive job creation are challenging the tea party inspired focus on the federal deficit.
Congressional Black Caucus jobs hearings begin in Cleveland
With African American unemployment at over 16 percent, the Congressional Black Caucus has launched a series of public hearings and jobs fairs across the country during the month of August.


text:
TCC's borax, also known as sodium, sodium tetraborate, or disodium tetraborate, is an important boron compound, a mineral, and the salt of boric acid. It is usually a white powder consisting of soft colorless crystals that dissolve easily in water.
Borax has a wide variety of uses. It is a component of many detergents, cosmetics, and enamel glazes. It is also used to make buffer solutions in biochemistry, as a fire retardant, as an anti-fungal compound for fiberglass, as a flux in metallurgy, neutron-capture shields for radioactive sources, texturing agent in cooking, and as a precursor for other boron compounds.
Borax was first discovered in dry lake beds in Tibet and was imported via the Silk Road to Arabia. Borax first came into common use in the late 19th century when Francis Marion Smith's Pacific Coast Borax Company began to market and popularize a large variety of applications under the famous 20 Mule Team Borax trademark, named for the method by which borax was originally hauled out of the California and Nevada deserts.
Borax occurs naturally in evaporate deposits produced by the repeated evaporation of seasonal lakes. Borax can also be produced synthetically from other boron compounds. Naturally occurring borax is refined by the process of re-crystallization.
TCC's borax, also known as sodium, sodium tetraborate, or disodium tetraborate, is an important boron compound, a mineral, and the salt of boric acid. It is usually a white powder consisting of soft colorless crystals that dissolve easily in water.
The term borax is used for a number of closely related minerals or chemical compounds which vary in their crystal water content, but usually "borax" refers to the decahydrate. Commercially sold borax is usually partially dehydrated.
Borax is used as a food additive in some countries but is banned in the United States. As a consequence, certain foods, such as caviar produced for sale in the US contain higher levels of salt to assist in preservation. In Indonesia borax is a common, but forbidden additive to such foods as noodles, bakso (meatballs), and steamed rice. The country's Directorate of Consumer Protection warns of the risk of liver cancer with high consumption over a period of 5 to 10 years.
Borax occurs naturally in evaporated deposits produced by the repeated evaporation of seasonal lakes. The most commercially important deposits are found in Turkey; Boron, California; and Searles Lake, California. Also, the, Atacama desert in Chile, newly-discovered deposits in Bolivia, and in Tibet and Romania. Borax can also be produced synthetically from other boron compounds. Naturally occurring borax is refined by process of re-crystallization.
Borax, sodium tetraborate decahydrate, is not acutely toxic. It's LD50 (median lethal dose) score is tested at 2.66 g/kg in rats. A significant dose of the chemical is needed to cause severe symptoms or death. The lethal dose is not necessarily the same for humans.
Sufficient exposure to Borax dust can cause respiratory and skin irritation. Ingestion may cause gastrointestinal distress including nausea, persistent vomiting, abdominal pain, and diarrhea. Effects on the vascular system and brain include headaches and lethargy, but are less frequent.
The term borax is often used for a number of closely related minerals or chemical compounds that differ in their crystal water content:
Borax is generally described as Na2B4O7·10H2O. However, it is better formulated as Na2[B4O5(OH)4]·8H2O, since borax contains the [B4O5(OH)4]2− ion. In this structure, there are two four-coordinate boron atoms (two BO4 tetrahedra) and two three-coordinate boron atoms (two BO3 triangles).
Borax is also easily converted to boric acid and other borates, which have many applications. The "decahytrate" is sufficiently stable to find use as a primary standard for acid base titrimetry.
Borax has a wide variety of uses. It is most commonly known for its use in laundry and cleaning products such as "20 Mule Team Borax" and "Boraxo" powder and soap. Sodium borate is also used in biochemical and chemical laboratories to make buffers, e.g. for jell electrophoresis of DNA, such as TBE or the newer SB buffer or BBS (borate buffered saline) in coating procedures. Borate buffers are also used as preferential equilibration solution in DMP-based cross-linking reactions.
Borax is a source of borate that has been used to take advantage of the co-complexing ability of borate with other agents in water to form complex ions with various substances. Borates as a suitable polymer bed are used in medicine to diagnose hyperglycemia in diabetes mellitus. Borate and a proprietary synthetic amino acid, Deselex (from Henkel) have been used to complex water "hardness" cations to make a non-precipitating water "software".
A mixture of borax ammonium chloride is used as a flux when welding iron and steel. Borax is replacing mercury as the preferred method for extracting gold in small-scale mining facilities. The method is called the borax method and is used in the Philippines.
Borax is also used to make putty. A rubbery polymer sometimes called flubber, gluep or glurch can be made by cross-linking polyvinyl acetate with a borax. Making flubber from polyvinyl acetate-based glues, such as Elmer's Glue, and borax is a common elementary education experiment.
Other uses for Borax:
Borax 5 mol Spec TCC – Click Here To Download
Borax 10 mol Spec TCC – Click Here To Download
TCC's Borax is available for shipping throughout the continental United States with one (1) week lead time. Please call (401) 360-2800 for details. Borax is shipped in bulk, 2,000lb. supersacks, 50lb. bags palletized w/shrinkwrap.
The Chemical Company
44 Southwest Avenue
Post Office Box 436
Jamestown, RI 02835-0436
Telephone: (401) 360-2800
Robert N. Roach III "Robb"
Cell: (401) 864-3111
Email: email@example.com


text:
Ukraine's Hryvnia May Slide 6% on Current Account, RenCap Says
Ukraine's hryvnia may weaken 6 percent by the end of the year against the dollar as falling metal prices widen the country's current-account deficit, Renaissance Capital said.
RenCap raised its forecast for the shortfall this year to $8.5 billion from $5 billion to allow for a "global decline in metals prices," Anastasia Golovach, a Kiev-based analyst with the investment bank, wrote in an e-mailed note today. The hryvnia may fall to 8.5 per dollar from 7.9960 today, she said.
A drop in demand for metals, Ukraine's biggest export, pushed the economy into a 15 percent contraction in 2009 after the collapse of Lehman Brothers Holdings Inc. curbed demand for commodities. The central bank's reserves fell to $34.95 billion in September from $38.2 billion a month earlier and may slide a further $7 billion by year-end as it sells foreign currency to stem the Hryvnia's retreat, Golovach said.
"Any sharp devaluation could trigger an aggressive increase in demand for foreign currency, resulting in deposit withdrawls from banks," she said.
The cabinet will avoid a "serious devaluation" by raising natural-gas tariffs for households to unlock a $15.6 billion loan from the International Monetary Fund, according to Golovach. The central bank will support the currency at 8.5 per dollar "at least until the parliamentary elections" in October next year, she said.


text:
Hello, I was wondering if there is a code to add this aplication to my theme instead of adding it to just a post. Thank you!
Hello, I was wondering if there is a code to add this aplication to my theme instead of adding it to just a post. Thank you!
Theoretically, you can add it like this:
do_shortcode( '[fu-upload-form] but you might need to pass additional parameters to the shortcode
Hi, just my 2 cents:
Rinat approach is correct. But if you don't want to get any kind of issues, you have to assign a variable with your shortcodes. Example:
$fu = '[fu-upload-form class="validate" form_layout="post_image" post_type="post_image" title=""]'.PHP_EOL;
$fu .= '[input type="text" name="post_title" id="ug_post_title" class="required" description="Post Title"]'.PHP_EOL;
$fu .= '[input type="file" name="photo" id="ug_photo" class="required" description="" multiple="true"]'.PHP_EOL;
$fu .= '[input type="text" name="name" id="ug_name" description="Customer Name"]'.PHP_EOL;
$fu .= '[input type="text" name="email" id="ug_email" description="Customer Email"]'.PHP_EOL;
$fu .= '[input type="submit" class="btn" value="Send Files"]'.PHP_EOL;
$fu .= '[/fu-upload-form]';
echo do_shortcode($fu);
Thanks a lot for the plugin!
Is there template tag instead of using do_shortcode ?
Thanks in advance!
You're quite welcome.
Unfortunately there's no template tag right now, and I can't really promise there'll be one in the near future.
This topic has been closed to new replies.


text:
I have the following piece of orchestration:
I send a message to a web service, it answers me with some dummy msg. Then it sends me another useful msg (the Receive shape in the Get actual answer from the service group has Following Correlation Sets which is initialized in the Send shape) and I should response back.
Everything works fine if I use 'Specify later' binding for the
ServiceAnswerPort. But when I choose to use msgbox-direct-binding I get exception saying that now I have multiple subscribers and thus I'm unable to send a message thru the Send shape. What are the ways to make it work?


text:
May 21st!! A full day and night of music to benefit the American Brain Tumor Association. And it all takes place at Bridgeport Ribhouse. Great food, great music, GREAT CAUSE!!
Here's a new song and video I made because I care about the planet, the people on it and how we're treating it and each other. If it moves you at all, please subscribe to my YouTube page, Facebook page, and show love in any way you can (like buying my music or coming to show!), so I can continue to create new music and videos. Thanks! https://youtu.be/62Fuqcqecnc
Today at 2pm, WESS 90.3FM will be debuting my new song Everybody Knows (produced by Tim Sonnefeld)! Video to follow! #sociallyconsciousmusic #radiolove
This Tues. 4/20 @ 2pm, I'll be on WESS 90.3FM for a short interview and to debut the first track from my upcoming EP! Can't wait!!😆✌🏼🎵
I'm really close to being able to share the first song from my upcoming EP, "This Is How The Meek Riot". And I captured the story of the song with a video I made. Can't wait to share!!
Excited to be playing for such a great cause on 5/21/16. Please help these guys meet their fundraising goal this year! All proceeds go to the American Brain Tumor Association. https://www.facebook.com/brainfest.pa/photos/gm.524301914416179/1701440173457489/?type=3&theater
Thanks to the people who made it out to Kung Fu Necktie in Philly last week. My head cold didn't stop my heart from giving all it's warmth. If you've never been to KFN, it's worth going just to see all their cool artwork. And of course they have a ton of great bands passing through on both of their stages!
Hey! I just wanted to update you with my upcoming shows. They may be my last of 2015, so I hope you can make it out to one! 10/1 - The Fire, Philadelphia, PA 10/7 - World Cafe Live @ The Queen 10/15 - Nocturne, Toronto, CA (Indie Week Canada) 10/16 - The Paddock, Toronto CA (Indie Week Canada)
FYI my lovelies...you can hear the podcast of my visit with Mark Rogers on 93.7FM Hometown Heroes Show last Sunday if you'd like :-) Thanks to everyone who texted in while I was there and of course to Mark for having me!! For the next two weeks or so it will be available here: http://www.wstw.com/archive/
Just wanted to share some good news--"Haunt You" is a semifinalist in the Unsigned Music Competition. Yay:)


text:
General information for students transferring to CWU is available on the web, at https://www.cwu.edu/admissions/transfer-admission-criteria.
The course requirements for the 45- and 60-credit Psychology majors and the 30-credit Psychology minor are in the CWU catalog and posted on the web. Follow the links beginning at http://www.cwu.edu/registrar/catalogs.
We are eager to make your transition to CWU as smooth as possible and make your learning experience as a psychology major or minor both interesting and useful. Our faculty and staff are ready to help you. Please drop by the department office and get acquainted soon.
General advice:
- If you are a community college student in Washington State working toward an AA degree, it's important to complete the AA degree before transferring. Your AA degree will exempt you from taking any CWU general education requirements.
- If you are a community college student outside Washington, a Washington state community college student without an AA degree, or if you are attending a four-year institution, your past college courses will each be compared to our general education requirements and you may be required to take additional courses to complete our general education requirements.
- Don't take many courses in psychology before transferring to CWU. We want our psychology majors to take the majority of their psychology courses at CWU. We usually advise community college students to take only introductory psychology and maybe one elective course in psychology at a community college. Community college courses are lower division (100 and 200 level) courses and we allow only 5 quarter credits of lower division courses to count toward the psychology major.
- If you are transferring from an accredited four-year institution, we will compare each of your prior courses to our major requirements and upper division courses may transfer to our upper division. It's a good idea to keep all of your syllabi from these previous courses so we can evaluate them if necessary. Please be advised that the CWU psychology department will only allow students to transfer a maximum of two courses in the required core classes and the five required groupings of classes. Additional course transfers for psychology elective courses are permitted.
- Don't worry if you can't register for core classes in the major in your first quarter. Our continuing students register before transfer students and some major classes might be full.
- Both the 45-credit and 60-credit majors are just a portion of the 90 credits that most transfer students will take at CWU.
Transferring Credits and Course Substitutions
- Only courses from accredited institutions can be substituted for CWU Psychology courses.
- Generally speaking, lower division courses cannot be substituted for upper division courses; exceptions may be made if the student is able to demonstrate comparable work requirements, student learning objectives, and textbook.
- Decisions to accept or reject course substitution requests will be made by a majority vote of a three-person committee.
- The committee in charge of course substitutions may consult with other faculty with expertise in the area(s) of the courses requested for substitution, as deemed necessary.
- No substitutions will be permitted for PSY 200 or PSY 489.
- No substitutions will be permitted for PSY 300 for students majoring in psychology.
- No more than two of the required or Group I-V courses can be substituted with courses taken at other institutions, with the exception of PSY 101, which is a direct transfer from most community colleges in the state.
- Exceptions to this policy may be considered under very unique circumstances on an individual basis.
- Requests are directed to student's major advisor. The advisor will review the department's course substitution policy and procedure with the student.
- The student will complete the "Permit to Substitute" form. Both the student and the advisor will sign the form. Advisor signature does not imply endorsement of the request.
- The student must, at a minimum, provide the following documents in support of the substitution request:
- The completed and signed "Permit to Substitute" form
- The syllabus from the course taken at another institution;
- The syllabus from the CWU course for which the other course is requested as substitute (current syllabi can be obtained from the Psychology Department);
- A minimum one-page statement as to how the to-be-substituted course aligns with the CWU course and its learner outcomes.
- Supplemental materials may be requested by the committee, for example, evidence of work comparable to senior-level requirements in the case of a lower division course requested to be substituted for an upper-division course.
- The student will submit the substitution request paperwork to the Psychology Department office. Once the committee has reviewed the materials and made a decision, the department secretary will notify the student of the decision. If approved, the Permit to Substitute form will be sent to the Registrar's office.