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An unidentified male in his late teens has been seriously injured in a hit-and-run bicycle accident in Anaheim, according to a news report.
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From pregnancy to motherhood, every mom has questions or could use some support. Join the conversation to learn from or help other moms just like you.
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I just wanted to know if I was the only teen and If my mom was the only young grandma.
young mom no at all ther is many young teen moms out there i found out i was pregnant at 17 and had my little girl at 18 and now she is 7 months and things are very hard and complicated but being an mom is the greatest thing ever.
Well I'm a teen mom. I found out I was pregnant on my 19th birthday & I just had her a week ago, I'll be 20 in December. :)
congrats on your baby girl, she is very adorable i remember whenmine was thissmall ... i turn 19 next month
I am so glad that you three found each other! How cool is that? :) What is the best thing that you have found about being a mom? What is hard? How do you cope with those hard days? Awesome job of reaching out Sincerityisourserentiy.
Nope. I found out I was pregnant when I was 17 and had my son at 18. I graduated high school pregnant and had my son 2 months later. He's now 5 weeks old and I couldn't be happier!
Awesome! Thanks for sharing eh3049! :)
I found out I was pregnant at 18 and I had my daughter when I was 19. I am now 20 and my daughter is 1.
And your momma is not the only young grandma.....I have a friend who was a grandma at 35. Now that's young and won't be me!!! I think that's the problem these days grandma and grandpas are not the same as they used to be sitting in a rocking chair, knitting, making home cooked meals and messing around in their gardens. This new generation of grandparents are still into clubs and partying, etc.
Our younger grandma's must then have more energy for babysitting, right?! :) -Jess
I'm 16 my daughter is 2 months
Hi, I too was a teen mom many years ago (lol) and I had my first child when I was 17yrs old. I can say being a parent at any age is not an easy job. I've had my struggles and you will too but in the end it will be all worth it. It's about what you make out of your situation and how you can be the best parent you can be. I like to say I was not the stereotypical teen parent that you hear all the negative information about. I'm 31yrs old expecting my fourth child and proudly the parent of 3 respectful straight A&B students.
Don't think of the negativity you and your parents may receive from others. That and your beauty baby to be will be your driving force to help you succeed.
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12th Annual Angel Tree Christmas Party to benefit 200 local children whose parents are incarcerated. They will receive food and clothes at Community Outreach Ministry Celebration. Gifts will be presented to the children letting them know they are not alone or forgotten, but are loved and valued by their community.
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12th Annual Angel Tree Christmas Party
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I had my iphone with Bb 4.6, 1.1.4 Baseband 4.02.13_G, I tried to use the Winpwn tool to get the custom logos, so I also used the Bootneuter, after Winpwn was complete, I could'nt get any signal, and if I did it was for just a couple minutes.
Note: If I turn the aeroplane mode on, and turn it off again, I get signal but for a few minutes, then just appears the carrier logo, but no bars, or just says no service. As same if I reset the Network settings.
I tried everything, I restored it to the 1.1.4 , then I used Iliberty, Ziphone still didn't work, I tried with 1.1.3, same didn't work.
Please if someone could help me out!!!


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Avions Caudron C.460
1929 Hamilton Metalplane
Jack Kearby's S.E.5a Replica
Vintage Aero Flying Museum's WWI replicas
"As always at AirVenture, history will be on full display through vintage aircraft," said Adam Smith, EAA vice president of membership. "These re-creations are products of a vision, hard work encompassing thousands of hours, and a passion for keeping the spirit of these pioneers alive for aviation enthusiasts to enjoy for generations to come."
Building of the replica 1909 Bleriot XI monoplane, the same model Louis Bleriot flew over the English Channel more than 100 years ago, was completed recently after three years of work by EAA staff and volunteers. The engine is an original three-cylinder radial Anzani "Fan-Type" (W-3), originally built in 1910 and re-built by EAA volunteers. During the past two conventions, the Anzani was fired up during daily engine runs in the EAA Workshops area while volunteers worked on the airframe in a nearby tent.
Another replica with French ties, an Avions Caudron C.460 racer, will appear for the first time after its completion in 2009. Mark Lightsey and his colleagues at Aerocraftsman, Inc., along with recent graduates of the Wathen Aviation High School in Riverside, California, built the aircraft, which was funded by EAA President's Council member Tom Wathen. The sleek replica is the same size and design to the original, which flew for the first time in 1934. The only change is the engine, which is a Czech 260 hp LOM M337CE in place of the original 6-cylinder Renault Bengali.
The one-of-a-kind Hamilton H-47 Metalplane, recently purchased at auction by Seattle businessman Howard Wright III for $671,000, will appear at AirVenture as the only surviving airworthy version of 29 built. The 1929 aircraft underwent a three-year restoration that finished in 1975 and features a Pratt & Whitney Hornet engine. It won the Reserve Grand Champion Antique award during that year's EAA Fly-In, as well as other prestigious awards.
"It's been 35 years since the Hamilton Metalplane was displayed in the Antique parking area during the EAA Convention, and we're pleased that Mr. Wright has decided to share the airplane with AirVenture attendees before the airplane is moved to the northwestern United States," said H.G. Frautschy, EAA Vintage Aircraft Association executive director. "The Hamilton will be parked in the vicinity of the VAA's Red Barn headquarters, just south of AeroShell Square."
Attendees will also have the opportunity to see five World War I replicas, including four courtesy of Vintage Aero Flying Museum of Hudson, Colo. – a Fokker Dr.1 triplane, Fokker D.VII, Fokker D.VIII, and a 7/8-scale Royal Aircraft Factory S.E.5a. Additionally, a sampling of artifacts, including uniforms, decorations, and logbooks, from both sides of the war also will be displayed.
They will join the S.E.5a replica already in Oshkosh. Jack Kearby, who spent almost 12,000 hours over six years creating this replica, flew the famous World War I fighter from Tulsa, Okla., into EAA's Pioneer Airport on May 27. The replica features some original parts, including a Vickers machine gun, landing gear wheel rims, windshield, and a WWI-era Hisso engine.


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Keep your pet off the hard ground with an elevated, waterproof pet cot that offers cooling comfort. The easy-to-assemble K&H Pet Cot features a durable, rust-resistant metal frame and waterproof 600-denier border fabric for incredible durability - indoors or out. Its elevated design and breathable mesh sleeping area help encourage good airflow and heat escape to keep your pet cool - even on the warmest days. This pet cot can be disassembled and taken anywhere - great for travel, camping, dog shows and more. Nonskid rubber feet keep this bed sturdily in place. For indoor or outdoor use. Cover is machine washable. Medium Cot measures 32" x 25" x 7" high; Large Cot measures 30" x 42" x 7" high.
Please click on "More Information" for care instructions.


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300 S Doheny Dr, Los Angeles, CA | Directions 9004834.072570 -118.389727
Daily 24 hours
Neighborhoods: Central LA, Beverly Center
A Mexican spa treatment called the Punta Mita at the Four Seasons BH is designed to improve circulation, aid digestion, and relieve fatigue. Perfect for Cinco de Mayo!
A classic Beverly Hills establishment, the Four Seasons oozes luxury and charm!
This is the Pretty Woman hotel. Even if a stay isn't in the budget, come here for star sightings, a peek at the breathtaking floral displays and a stroll up intersecting Rodeo Drive.
Needing a massage after an expensive massage! – I usually get massages at Burke Williams at least once a month and decided to try the manipura experience at the four seasons hoping to become a regular. It started with the scrub which was fine but when it was time for the massage I was very disappointed. I was already so tense and stressed and was hoping for a great massage. There was hardly any massaging done. More like poking. My back would be poked for 10 seconds on one place than another place. I actually had to say something during the massage bc I was so upset. I even made a comment to the receptionist when asked how the experience was and all he said was i will be sure to let her know - we love to hear the feedback. I guess they don't want a regular. Plus it's not cheap! $260 (not including tip). Needless to say, unless I get one hell of a discount for coming and trying again-I will not be returning!
the best place in town to have a drink – I never stayed in this hotel but the food is amazing
Service Is Everything – The treatment was good but if you care about customer service, you'll most likely fare better at the Motel 6.
FOUND: Massage therapist extraordinare – As a woman, I was a bit nervous to be massaged by a man. However, I was referred to David May at the four Seasons Hotel and it was the most relaxing massage I've ever experienced. He was so polite, so professional, and has magical hands. I was completely rejuvenated and revived. I have been back several times and each time is an amazing experience. I am a hairdresser and on my feet all day so I really need a massage therapist who can meet all my physical healing needs and David May is just the one to get your body just like new.
I am hooked on this treatment and will be a forever client of Davids. I highly recommend David to anyone who loves to be treated like royalty and needs TLC.
F & F........FABULOUS FACIAL! – The receptionist assisted me with answering my questions when I called she said my facialist was great and gets booked fast, I thought we will see. Of course everyone knows the Four Seasons is the place to be in the city for cocktails and celebrity sites but I had no idea how comfy, cozy and personable the spa was. Waited in the lounge for my "dermal roller facial" a new treatment exclusively to the spa. Therapist would pass each other and say hello to each other and to guest and ask if we wanted water, tea or fruit. My facialist came around the corner with a smile like sunshine and introduced herself hello, I'm Jamilla. WoW! her skin was gorgeous I asked will my skin look like yours when I'm done? We both giggled :-). We went it a room called Gardenia she told me to make myself comfy and I did. Examining my skin with her warm&confident hands she asked me questions about my skin&daily maintenance which I was very impressed. Jamilla explained the derma roller facial was from leaf&rusher in beverly hills and showed me the incredible tool that was gold and had little points on the roller, it is made by balinese artisans & simulates balinese massage. the roller stimulates the skin, improves the penetration of the product as well as improves my complexion diminishing fine line and wrinkles my type of facial! Jamilla proceeded to cleanse and nourish my skin as I slipped in & out of consciousness her touch was so nurturing & healing while the mask was on she massaged my feet I had to ask her are u a massage therapist? she said yes bingo! A esthetician & massage therapist I am her client for life! she worked on my arms, neck & scalp I was totally mesmerized. Placing a serum on my skin that felt like a veil of silk, my skin was so light as she rolled the roller on my skin it felt a bit pricklely,explaining to me what direction and pressure to use. I had to give her a hug it felt as though I had a transformation. Definitely 5 star I highly recommend Jamilla she is amazing.
Intimate atmosphere, personal touch and top notch therapists! – I've been to several five star spas throughout the Los Angeles area and the Four Seasons Spa is my favorite by far. For me, one of the most important aspects of my spa experience is being able to immediately speak to a spa receptionist, and not be put on hold with annoying zen music for 20 minutes before an uninformed agent gets on the phone who knows nothing about the therapists at the very spa they work at. This is not the case at the Four Seasons. The reception staff is professional, courteous and well versed in their menu. I dealt with Maki, and she was able to make several recommendations to enhance my experience. She recommended a facial with Dallas and a massage with David. Dallas knew immediately what my skin needed and tailored my facial to get the results that I wanted. Her extractions were as painless as possible and I was very happy with how my skin looked when I left the spa. After my facial, I had a deep tissue massage with David. I really cannot put into words how amazing I felt after my massage! A lot of the time when I go to spas I feel like I am getting a standard routine massage, but David personalized my massage and as a result, relieved every sore spot on my body. I cannot recommend him enough! A couple of drawbacks to the spa are that the locker rooms are small, you cannot use the pool facilities and the valet took a bit too long, but I'm definitely willing to sacrifice those amenities to be assured of amazing therapists and fantastic treatments. The Four Seasons Spa is a great find in L.A.
Great customer service – I was extremely impressed with my stay at the Four Seasons. The hotel is super upper class and my wife and I had an amazing time. The concierge, Heather Holmes, gets an A+ in my book for how she took care of us. My wife knows that she deserves the best and I love to treat her to a little high end shopping every now and then. We went to Dior, Louis Vitton, and I even drove her out to the beach to get a new 18K Gold navel ring at Tommy T's Body Piercing. She loved it! As far as the services offered at the Four Seasons, I always think you get what you pay for. I used the internet access and I really don't mind the $10 fee. There are certain things I expect with my room and they were all great, but if I need anything else I don't mind paying the extra small amount of money. The whole price was well worth our stay. Very classy!
very effective treatments not a regular fufu hotel spa – the messages are amazing along with the body treatments, incredible wraps! the service is outstanding but overall a manicurest named GINIA gives the all time incredible pedicures never have i had a better one and ive been around the world, always coming back to her. i have somewhat problematic skin and searched many spa's for an esthetician that could help me with my skin and never did was i as impressed with the results of my facials untill DALLAS was reffered to me by a friend. she cleared up my face and has helped me find an incredible regime to prevent my skin from ever breaking ut, she knows skin better than any esthetician ive been treated by. if your looking for a fabulouse pedicure or have been having problems with your skin the four seasons spa is where you need to go, unlike any other hotel spa that treatments are effective and the results outstanding.
Overall good customer service – I had a convention and most of us stayed at the Four Seasons. I booked a massage and a pedicure/manicure service. The massage was the worst massage I have ever had, and before then I would say that is an oxymoron....How can you receive a bad massage? Well....let me tell you, you can! It was the most uneven, massage I have ever received, and I could not wait for it to be over. Luckily they redeemed themselves with my manicurist Ena. She was wonderful, and more than made up for the first lousy hour of my visit. Other than that, the customer service was very gratious, and I would go back, but I would definitely not have the same person again. Funny thing is, I ran into another young lady today, that said she felt the same way about a massage she had received at this establishment as well. So, I knew that it wasn't just me. An establishment that is so well known for catering to their customers needs, should make sure that every individual is qualified to give massages at this grand location.
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Looks like a Watch, works like the ultimate survival tool! You don't want a sparkly "showpiece" hanging on your wrist when push comes to shove. Luckily, this rugged Tech4o® works as well as it looks. A stealthy black aesthetic is complemented by a high-visibility reversed display and no-glare mineral glass.
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text: Dancing With the Stars Season 16 Cast Meet the New Cast of Dancing With the Stars by Maggie Pehanick 3/12/13 5 Reactions 11 Shares Print 5 3 3 0 0 Prepare your Dancing With the Stars ballots, because the cast of season 16 is here. As usual, the floor will be filled with reality stars doing the merengue, waltzing country crooners, and football players perfecting the salsa. The new season premieres March 18, but until then, check out the show's 12 new contestants — including new addition The Bachelor Sean Lowe! — and weigh in on who you're already rooting for! Source: Getty Wynonna Judd What's Your Reaction? 0 0 0 0 0 0 How you know her: The country singer has won multiple Grammys during her decades-long career. Her DWTS stereotype: The country star coming to reclaim the spotlight. Her partner: Tony Dovolani Source: Getty 1 / 12 D.L. Hughley What's Your Reaction? 0 0 0 0 0 0 How you know him: The comedian starred on his own TV show, The Hughleys. His DWTS stereotype: The funny guy who wants to prove he's got moves. His partner: Cheryl Burke Source: Getty 2 / 12 Ingo Rademacher What's Your Reaction? 0 0 0 0 0 0 How you know him: The TV actor plays Jax on General Hospital. His DWTS stereotype: The soap star who brings sex appeal to the dance floor. His partner: Kym Johnson Source: Getty 3 / 12 Andy Dick What's Your Reaction? 0 0 0 0 0 0 How you know him: The comedian has starred in many movies and television shows, most notably NewsRadio. His DWTS stereotype: The wacky comic who brings the candid moments. His partner: Sharna Burgess Source: Getty 4 / 12 Kellie Pickler What's Your Reaction? 0 0 0 0 0 0 How you know her: The country singer rose to fame after appearing on the fifth season of American Idol. Her DWTS stereotype: The beautiful singer who is no stranger to strutting in sequins. Her partner: Derek Hough Source: Getty 6 / 12 Dorothy Hamill What's Your Reaction? 0 0 0 0 0 0 How you know her: The 56-year-old Olympic figure skater won the gold medal in 1976. Her DWTS stereotype: The aging athlete who can keep up with the kids. Her partner: Tristan MacManus Source: Getty 6 / 12 Victor Ortiz What's Your Reaction? 0 0 0 0 0 0 How you know him: He's a former welterweight boxing champion. His DWTS stereotype: The athlete with something to prove. His partner: Lindsay Arnold Source: Getty 7 / 12 Zendaya Coleman What's Your Reaction? 0 0 0 0 0 0 How you know her: She's the star of Shake It Up! on the Disney Channel and is currently recording an album. Her DWTS stereotype: The princess. Her partner: Val Chmerkovskiy Source: Getty 8 / 12 Jacoby Jones What's Your Reaction? 0 0 0 0 0 0 How you know him: He plays for the 2013 Super Bowl champs, the Baltimore Ravens. His DWTS stereotype: The football player with swagger. His partner: Karina Smirnoff Source: Getty 9 / 12 Aly Raisman What's Your Reaction? 0 0 0 0 0 0 How you know her: She was the captain of the gold-medal-winning 2012 Olympic women's gymnastics team. Her DWTS stereotype: America's sweetheart. Her partner: Mark Ballas Source: Getty 11 / 12 Lisa Vanderpump What's Your Reaction? 0 0 0 0 0 0 How you know her: The restaurant owner is one of the stars of The Real Housewives of Beverly Hills. Her DWTS stereotype: The reality star with attitude. Her partner: Gleb Savchenko Source: Getty 11 / 12 Sean Lowe What's Your Reaction? 0 0 0 0 0 0 How you know him: He just got engaged after a long season romancing ladies on The Bachelor. His DWTS stereotype: The reality heartthrob. His partner: Peta Murgatroyd Source: Getty 12 / 12 5 3 3 0 0 Back to Homepage Comments Read More Lisa VanderpumpAndy DickKellie PicklerDancing With The StarsTV Dancing With the Stars Speed Read: Did Courtney Love Really Solve the MH370 Mystery? 3/18 Our Super Scientific Method Predicts Who Will Win DWTS 3/13 We Already Know Who Will Win DWTS This Year 3/06 Meet the New Cast of Dancing With the Stars! 3/04 All the Training That Helped Meryl Davis Reach Gold 2/19 Corbin Bleu: DWTS Is Like "Having a Second Girlfriend" 11/18/13 Kellie Pickler Taylor, Carrie, and Hayden Shine on CMA Awards Red Carpet 11/02/12 Who Do You Think Was Worst Dressed at the CMA Awards? 11/11/10 Taylor, Carrie, Sheryl, and Hayden Meet John Mayer's Ab Situation at the CMT Awards! 6/10/10 When Celebrity Powder Puffing Goes Wrong 4/01/10 Who Was Your Favorite New Celebrity Pet in 2009? 12/01/09 Taylor, Brad and Kid All Hit the CMT Stage in Style 6/17/09 Andy Dick If You Had To... 7/17/08 Andy Dick Wants Jessica Simpson 5/21/07 Andy Dick Was TomKat's Wedding Planner? 4/20/07 Andy Dick Is... Jacques Cousteau 4/12/07 VH1's Best Weed Ever! 4/11/07 Meet The Presidential Speechalist 1/24/07 Lisa Vanderpump Photos From RHOBH's Lisa Vanderpump's Daughter's Wedding 8/31/11 Paris and Nicky Celebrate Their Aunt Kyle's Real Birthday! 1/12/11 Get a Personalized Celebrity & Entertainment Newsletter! Help us customize your newsletter by selecting your interests (optional): TV Celebrity News A Listers Young Hollywood What's Your Reaction? 0 0 0 0 0 0 0Reactions Link Love Meet the cast of Jem and the Holograms — The Hollywood Reporter Katy Perry's "Birthday" video is too much to handle — HuffPost Entertainment 10 things you need to know about The Quiet Ones — Moviefone 5 iconic pop culture moments that weren't in the original — Cracked Is the Bad Teacher TV show any good? — BuddyTV Yes, a new Sisterhood of the Traveling Pants movie is really happening — Gossip Girl The comedians who made it onto Time's 100 Most Influential People list — Splitsider Cameron Diaz's 10 best movies of all time — Rotten Tomatoes Robert Downey Jr. + Mark Ruffalo = #ScienceBros — POPSUGAR Tech


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Denver >Food & Dining > Tamayo
1400 Larimer, Denver, CO | Directions 8020239.747307 -104.999374
Mon-Fri 11:30am-2pm, 5pm-10pm
Sat 5pm-10:30pm
Sun 5pm-10:30pm
Neighborhoods: LoDo, Northwest Denver, Union Station
Provided by CityGrid
Mon-Fri | 5-7PM join us at the Tequila Bar and Lounge for a complimentary Margarita Tamayo. Also enjoy $3 Tacos, Tortas, Sopes and $5 Margaritas and Latin Cocktails. Salud.
fancy and good but expensive
Recently, I was part of a large party that dined at Tamayo. I think that whatever the bill was for a party of over 25, was not worth whatever we paid. Service was exceptional and very attentive but the food was not that good. In fact, I cannot remember what I ordered except that it had green sauce. For a place such as this to be located on Larimer, one can expect much more from down the block.
We enjoy our time there whenever we feel like eating some Mexican food!
Customer service can be friendlier, and food is okay, but nothing to die for. overall, it is an OKay place.
Terrible – We arrived on time for our 8:15 reservation. Our table was not ready, so we headed to the bar area, where we easily found 4 seats at the bar. There were at least two bartenders, and I ordered our round. However, the first bartender basically forgot that he took the order. Ten minutes later, I ordered from a second bartender. Again, he was either distracted or perhaps not trained well enough, becasue he came back nearly 10 minutes later to take the order yet again. Considering we were ordering 2 beers and 2 vodka sodas, this should not have been an overly complicated process. We finally got our drinks around 8:45. We were finally seated for our 8:15 reservation around 9pm. The menu did look fabulous, so I was excited for our entrees to arrive. I ordered the pork tenderloin. When it arrived, the pork tenderloin was in slices, with the centers clearly RAW! I cook pork tenderloin often, and I don't mind the center to be pink in order to preserve the tenderness and juices of the meat. However, these pieces were literally as rare as a beef tenderloin ordered medium rare to rare. Luckily, there were two end pieces that were cooked well enough, because by the time we received our entrees, it was 9:45, and sending this back to the kitchen seemed pointless since I didn't want to sit there for another hour. In addition, to the pork not being cooked to an appropriate temperature, it was also very tough and not tender. My dinner mates also had very so-so reviews of their own entrees.
This was a disappointment. Service from the bar to the table was absolutely horrible, and the food was awful. I would rather make something at home. I would not recommend this to anyone, and while I realize that we were there on an "off" night, the experience was so unpleasant that I would not be willing to give it a second chance.
Great Food, Great Service – Food was delicious. The service is good and quick.
Excellent Food, Excellent atmoshphere!! – Great service, great food!! Our drinks never even were half empty. A little expensive, but worth every penny!!
Expensive and poor service – I'm not sure why we put ourselves through the experience with all the other options on Larimer. Would have done better at Teds for a fraction of the price. The staff is poorly trained and rude. As we entered there was a small mob of staff at the front of the restaurant, ignoring us...the CUSTOMERS. I don't know why I have this wild expection that we should be noticed when we walk in the door. I felt invisable...and there were so few other customers in the restaurant, I just don't understand why a restaurant would not train staff to fall all over customers in this type of economy. I always expect good service, but especially these days! You can tell when a restaurant puts service first. The wait person is attentive and is focused on you not gossiping with his fellow worker. If I wanted that treatment I would go to Safeway and let the checker ignor me. I wanted another glass of wine, but by the time we could get someones attention, I was too frusterated to bother and wanted to go. We went 20 yards away to Teds and finished the evening.
Food? Give me a break. The king has no cloths. There is nothing special other than the fact that you are paying too much Mexicanish food. I dine out 3x per week and will not return.
My prediction....they close in the next 6-8 months.
Excellent Mexican Food for a not to shabby price – Eating here was a real treat. My wife chose to bring me here and we had a delightful experience. People say cheap mexican food is horrible mexican food, but this place will turn that thinking around. Will be back soon!
Bad customer service, bland food, and $$$ – I've walked by Tamayo so many times on my way to UCD campus for school and saw their cool little salsa dip that so many people ordered. I decided to try it for my birthday on a Saturday. Big mistake. Reservations for the big party was unsure because Cirque de Soleil was in town so they couldn't guarantee us the reservation. My friend even had to give a credit card number to them to secure our spot.
The service started out good. The guac, salsa, and chips they brought out were delicious. I got there later than some of my friends and was informed that the server just asked my friends if they wanted chips and salsa. We didn't know the guac costs $9.50 for a small bit of guac!! If we had known that we would have just asked for chips and salsa with NO guac! We had a big party so they ended up giving up 5 of these.
The seating was nice, we got the rooftop patio. One of my friends is preggo and will not eat any undercooked food so she had to send her beef tacos back to the kitchen to get cooked. The meat still came back pink. I was there when she informed the waitress that she had lost her appetite and did not want the tacos anymore. The waitress seemed indifferent and did ask if she wanted anything else and when my friend said "no" the server just asked "are you done?" and whisked away her plate. She had even explained to the waitress that she is preggo and cannot eat undercookd food. Another friend had a custom meal which consisted of a steak and mashed potatoes which cost $32 dollars!
I ordered the lobster crepes because everything else seemed overpriced and didn't sound appetizing. The presentation was great but the taste was absolutely bland! I ate it all because I didn't want to waste it. It cost $15 for 2 small crepes and I didn't even taste the lobster. I also ordered the calamari which was not bad, it had flavor but it was probably fried and sauteed with the sauce it came it so it is "wet".
My friend wanted to make it special for me so she bought a cake for use. It costs $5.00 to cut one piece of cake!! It would have cost us almost $90 for that! But the guy who cut said he would waive the fee for us which was very nice. I was even supposed to get a "free" dessert but we were never asked if we still wanted it.
The bill came out to be $500 dollars including tip because we had about 17 people. The waitress forgot to take off the entree my friend could not eat and had to re-fix the bill, luckily we checked the entire bill to make sure they didn't cheat us. Just because we are a big party doesn't mean we should get bad service. We felt like the waitress could care less because she was getting her 20% anyway. I barely ate anything and I'm pretty sure that everyone else left hungry as well which is bad news for a restaurant. How did we spend $500 and leave hungry?!?!! I don't know if I would give this place a second chance. Tamayo would really need some work in customer service and food for their prices.
Fantastic meal and Service – My wife and I dined here for Valentines Day and it was by far one of the best meals we have ever had. They had a special menu for the holiday, and everything was delicious. The service was impeccable and the food was wonderful. I did find the dessert to be a little small, but when I mentioned it to our server, he brought out another piece for us to share. This was our first time at Tamayo, but it will surely not be our last.
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DEATH: Ida Lucille Wyss, 84
Ida Lucille Wyss, 84, playing piano and organ at area churches, died Oct. 19 at Salem Home in Hillsboro.
Services will be at 11 a.m. Tuesday, Oct. 23, at Ebenezer Methodist Church, three miles south, three miles west, on U.S. Highway 77, rural Burns.
Visitation is from 6 p.m. to 8 p.m. Monday, Oct. 22, at Zeiner Funeral Home, Florence.
Memorials may be made to Rivercross Hospice, Wichita, and sent in care of Zeiner Funeral Home, 104 W. Fifth St., Florence, KS 66851.
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I have hosted more than a couple people for this area. As of next Tuesday, I will be VERY free to hang out, or show some people around if you want or dont know anyone here, bored, etc. So hit me up and I would be happy to get back to you. Just thought I would put it out there.
Safe travels,
Safe travels,
All right. What about meeting Saturday morning at dottie's true blue cafe on 6th street for breakfast. Let's throw all ideas on one pile and find the best thing to do tomorrow. I'll be there at ten. Bring whoever you want. I would appreciate a quick message on this board if you want to come. Oh, plane is boarding... See you tomorrow. S
Hi all, sorry I havent been able to respond earlier. I would like to get together and even host, however, im going to be pretty tied up until next week now. I am going to be pretty free after Tuesday, however, because of all the time im going to have, I decided to take a last minute trip to Japan. I will be getting back next tuesday so anytime after that I will be free to hang out, host, etc. Sorry about that. Definitely anyone who wants to hang after next tuesday or those arriving in March hit me up.


text: Why GRTS? Gain a comprehensive, Christ-centered education from an accredited seminary. At the end of the day, after all the papers and exams, our focus at Grand Rapids Theological Seminary is to develop leaders who will guide others into life-changing relationships with Jesus Christ. Every class at GRTS centers on this principle, reminding students of their responsibility to feed the sheep of God's Kingdom. We're accredited by the Association of Theological Schools (ATS) and Higher Learning Commission (HLC). Enjoy flexible classes that allow you to still be an employee, a parent, a spouse, and a friend. Many of our students balance family and jobs and ministries while going through our program. Our block scheduling means that each class is only one day per week, leaving you time to still maintain other responsibilities without overwhelming yourself. We offer classes in the morning, afternoon, and evening—plus you can take up to eight of your courses online. Click here for an updated list of our Course Schedules. Discover the program that best suits your calling. GRTS offers a wide variety of programs, from licensed counseling to Ph.D. prep to ministry training. You can become knowledgeable in intercultural ministries or prepare for pastoral ministry with our Master of Divinity degree. Click here for a complete list of all our degrees and programs! Receive financial aid to help make your education affordable. More than ¾ of GRTS students receive financial aid. We offer grants and scholarships based on your financial need and the amount of credits you take. Develop a solid foundation of biblical and theological knowledge. Bible and Theology courses are an integral part of GRTS, allowing you to fully integrate Scripture. We are fully committed to preparing you to become a biblically and culturally informed leader in your workplace and community. Our interdenominational setting allows for honest discussion from many different backgrounds. Join a community and build relationships with your professors and peers. Our program averages a class size of 20 students, meaning discussion is active and inclusive as you wrestle with various theological and ministerial issues. You will have the chance to connect with the professors on a regular basis, helping you network for future job opportunities and ministry partners. Pursue ministry in a city that is booming with opportunities. As a city, Grand Rapids is well resourced with churches and social services agencies, including our own alumni. Whether you want to serve in a mega church or plant a house church, lead a homeless ministry or start your own non-profit…You will find it here in Grand Rapids! Prepare for a life after graduation with internships, ministry residencies, and thesis classes. Every program includes practical classes that will help prepare you for life after GRTS. If you are a counseling student, you will have 700 hours of practicums and internships. Ministry students will have four different residency classes. And students going on to further education will have the opportunity to create and defend a thesis for Ph.D. prep. Attend school in what Forbes magazine calls "The Best City to Raise a Family in the U.S." Grand Rapids has a rapidly growing downtown with an emphasis on arts and culture. We are just 40 minutes from Lake Michigan and near outdoor activities like camping, skiing, etc.


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Diptyque Olene Eau de Toilette -- 1.7 oz.
Made for both women and men, the Diptyque Eaux de Toilette use the finest raw materials to relate a childhood memory and poetically reinvent and enhance nature or a journey, real or dreamed of. Olene Eau de Toilette conjures up Venice, with its narrow streets, palaces of a thousand splendors, fragrant hidden gardens and alfresco dining at nightfall under the shelter of a pergola. It's a place where time simply ceases and is best explored on foot on unforgettable strolls, where each step echoes an aromatic accord. Venice is a city to inhale deeply: here, a thick cluster of wisteria; there, tender honeysuckle; further on, sensual jasmine and elegant and mysterious narcissus. Olene conjures the mysterious depths of twilight dotted with the subtle, starry glow of dainty white flowers.
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text: House Committee Chair Cites Resistance Concerns in Animal Drug House Rules Committee Chair writes to the FDA to suggest the rejection of an animal drug due to concerns about causing human resistance to similar drugs. Published on: Jan 11, 2007 Tweet Post to Your Wall. Email Story RSS Permalink Print The Food and Drug Administration should follow it's scientific advisory committee's recommendation and reject the animal drug cefquinome, U.S. Rep. Louise Slaughter, D-N.Y., said in a letter to the FDA. Slaughter, the Chair of the House Rules Committee, says the use of the drug, used to treat life-threatening infections in animals, could cause resistance to similar drugs in humans. "Given the recent outbreaks of E. coli and other food borne illnesses across the nation, it is hardly the time to ignore the advice of scientists, and potentially impair our ability to treat deadly infections," Slaughter says. The FDA's Veterinary and Medical Advisory Committee recommended on September 25 against FDA approval of cefquinome in cattle over concerns that it could make similar drugs less effective in humans. The drug's manufacturer, Intervet, has suggested it intends to continue to seek approval for the drug and has not withdrawn its applications to the FDA.


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You can also read manga online try the Summer Wars manga!
You're reading Summer Wars 5 online. You can try some of the related manga below or try some other manga from our manga list.
Users who read Summer Wars also read Peach!, Emma Bangaihen, Umi No Misaki, Onani Master Kurosawa, Orange Yane no Chiisana Ie and Love Comedy Style.


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Woman succumbs to crash injuriesFebruary 19,2013Woman succumbs to crash injuries
GUILFORD An elderly Dummerston woman died last week from injuries she received three days earlier in a car crash on Weatherhead Hollow Road.
Vermont State Police said Jean Anderson, 94, died at Dartmouth-Hitchcock Medical Center Feb. 10, three days after the Feb. 7 accident.
Police said Mary Evelyn Lunge, 62, of Hinsdale, N.H., said she fell asleep and hit a tree during the midday accident. Lunge received a concussion in the accident, as well as cracked ribs. Anderson was airlifted to the Lebanon, N.H., hospital for more serious injuries.MORE IN This Just InStaff Report Full StoryStaff Report Full StoryThe Associated Press Full Story
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- TOMORROW'S HEADLINES TODAY: Update on Center Alley project, review of opiate talk evening at the library and aldermen want kids to wear helmets while riding bikes around town.
- TODAY'S WEATHER MINUTE: Flood watch in effect for Rutland County, developing El Nino shaping up to be extreme this year, Don DeLillo on the season's warming trend and Veena catches digital sniffles, all in today's minute.
- TOMORROW'S HEADLINES TODAY: Dramatic river rescue in Southern Vermont, Castleton moving ahead on sale of old town office building, Passover celebrated in Woodstock.


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Margaret Bean Receives UO Libraries' Solari Incentive Award
Margaret Bean Receives UO Libraries' Solari Incentive Award
Submitted by ronr on Wed, 2013-06-19 11:44
Margaret Bean, head of the Science Library, has received a 2013 Solari Incentive Award for her proposal to establish a Science Boot Camp for librarians in the region.
The Solari Incentive Awards support project proposals that align with library's strategic directions and organizational interests and fulfill individual professional development goals.
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Mayor Vincent C. Gray has told Wall Street ratings agencies that he will not seek to dismantle the District's independent financial agency, a recognition that a trial balloon floated this year may have swiftly popped.
In a February meeting with Washington Post reporters and editors, Gray (D) suggested that the city's powerful chief financial officer ought to be directly accountable to the mayor. Currently, the chief financial officer is appointed by the mayor and confirmed by the D.C. Council to a five-year term and cannot be fired except for good cause.


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Hulda Charlotte Zabriskie
Circumstantial evidence from census and genealogical records proves the Zabriskie family traveled to Utah in 1851. Further research is needed to determine the name of the company they traveled with.
Her birth date is confirmed by the inscription on her gravestone.
Company Unknown (1851)Approximate age at departure: 7
- 1850 Iowa Census
- Source Location
- Family History Library, Salt Lake City, Utah
Find A Grave
- Source Location
- Internet Web Site
New FamilySearch/Family Tree
Utah Death Certificate Index, 1904-1961. Utah State Archives and Records Service.
- Source Location
- Internet Web Site


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Last year, the BCC launched the True Life: I'm an Intern video series to give students insight into what it's like to intern and how to be successful. If you missed that series, be sure to check it out on the BCC YouTube channel.
This spring we're launching season two of the True Life series! In part one, Aaron Konetzke discusses his experience at Procter & Gamble and his tips for making the most of an internship.
Find out more about Aaron and watch below to learn more about his internship:
Next week: Chase Ellman will talk about his internships at both Verve Talent & Literary Agency and Sony Pictures Entertainment in part two of True Life: I'm an Intern. Stay tuned!


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Bariatric surgery is the clinical name for weight-loss surgery and is prescribed as a treatment for obesity.
By surgically reducing the amount of food the stomach can hold, the body absorbs fewer calories. Following bariatric surgery, patients typically lose 50 to 70 percent of their excess weight (in a one- to four-year period). In 2009, approximately 350,000 people with morbid obesity had bariatric surgery in the United States.
The goal of weight-loss surgery is to improve health, enhance quality of life, and increase lifespan. People who choose bariatric surgery make a life-long commitment to a new way of life. If you believe bariatric surgery may be right for you, the compassionate team at the Bariatric Center of the Rockies will work with you to understand both the benefits and risks. Careful consideration should be taken before deciding to move forward with bariatric surgery.At the Bariatric Center of the Rockies, a leading bariatric center in Colorado, we offer three types of bariatric surgical procedures: gastric bypass surgery, LAP-BAND® surgery, and vertical sleeve gastrectomy. In fact, we are one of few programs in the state of Colorado to offer vertical sleeve gastrectomy. Each bariatric surgical procedure is typically performed laparoscopically, which means patients can go home sooner and they heal much faster. All three bariatric surgeries tend to be very successful but you will want to understand each procedure before moving forward as each type of surgery has certain advantages. If you believe bariatric surgery may help improve your health, enhance your quality of life, and increase your lifespan, please complete this secure information request form or call Michelle Carpenter, our patient navigator at the Bariatric Center of the Rockies. If you would like to learn more about why you should choose the Bariatric Center of the Rockies for weight-loss surgery in Colorado, please follow this link.
Please note: To qualify for bariatric surgery, specific criteria set forth by the National Institutes of Health must be met.
University of Colorado Health employees dedicate themselves to providing patients and other customers with world-class care and service. Outside organizations recognize that, calling University of Colorado Health's hospitals some of the best in Colorado and even the best in the nation. Some of those accolades are listed below:
University of Colorado HealthFort Collins, email@example.com


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Blossom Gray Patent-Fall 2012
Product DescriptionBlossom, inspired by the delicacy, layers and color of Mexican paper flowers, will have your little one ready for spring's first bud. This finely-crafted shoe in navy patent leather boasts a hand-cut fuchsia suede flower with green glitter center and green glitter cutout circles in the detailing. A classic shoe with floral flair!
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ROMNEY - West Virginia Gov. Earl Ray Tomblin recently announced that Romney, in Hampshire County, was awarded a $1 million Small Cities Block Grant to help upgrade its sanitary sewer system as part of the U.S. Environmental Protection Agency's Chesapeake Bay restoration program.
The total cost of the project is about $16.2 million and will benefit about 1,027 households, according to a news release from the governor's office.
Under its Chesapeake Bay "pollution diet," the EPA has mandated drastic reductions in the amount of nutrients - nitrogen and phosphorus - that can get into the bay via its tributaries. Nutrients are produced by waste. Sewer treatment plants remove nutrients from the wastewater before it is discharged.
Romney as well as all of the greater, eight-county Eastern Panhandle is in the Potomac River watershed, and the Potomac is one of the bay's major tributaries.
In addition to Romney's sewer system improvements, there are plans to upgrade about seven other sewer systems throughout Panhandle.
The total estimated cost to make improvements to all the systems in the Panhandle is close to $200 million, which includes improvements to sewer plants in Martinsburg, Berkeley County, Charles Town, Shepherdstown and Morgan County.
The Romney grant was one of 17 SCBG awards totaling more than $12.5 million for various infrastructure projects around the state, the news release states. The SCBG funds will leverage more than $66 million in other local, state and federal funds for the projects, which will serve about 12,200 residents or more than 5,480 households, according to the release.
SCBG funds come from the U.S. Department of Housing and Urban Development and are administered by the Community Development Division of the West Virginia Development Office.
- From staff reports


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The Charlotte R. Bloomberg Children's Center opened May 1, 2012. Take a look inside at what families and visitors can expect.Virtual Tour
Chief of Pediatric Gastroenterology and NutritionStermer Family Professor of Pediatric Inflammatory Bowel Disease
Brady 320600 N. Wolfe StreetBaltimore, MD 21287-2631
Dr. Oliva-Hemker currently sees patients at the Rubenstein Child Health Building in Baltimore and at the Greater Baltimore Medical Center in Towson.
Medical School: Johns Hopkins University School of Medicine
Residencies: Johns Hopkins Children's Center – Pediatrics
Fellowships: Johns Hopkins Children's Center – Gastroenterology
Dr. Maria Oliva-Hemker received her medical degree from the Johns Hopkins University School of Medicine. She completed her residency in pediatrics and did a fellowship in pediatric gastroenterology at Johns Hopkins and then joined the faculty in 1993. In 2008 she was appointed Chief of the Division of Pediatric Gastroenterology and Nutrition.
In 1998, Dr. Oliva-Hemker began the Pediatric Inflammatory Bowel Disease (IBD) Center at Hopkins, which provides evaluation and medical care to hundreds of children and adolescents with Crohn's disease and ulcerative colitis from all over the United States and beyond. She is the inaugural recipient of the Stermer Family Professorship in Pediatric IBD. Dr. Oliva-Hemker is an active clinical research who has co-authored numerous articles, book chapters and reviews and has served on multiple editorial boards. She is an editor-in-chief of the book "Your Child with Inflammatory Bowel Disease: A Family Guide for Caregiving."
Dr. Oliva-Hemker's clinical practice focuses on inflammatory intestinal disorders such as IBD, autoimmune enteropathy and eosinophilic gastroenteritis. Additional clinical interests include malabsorption syndromes and nutritionally-related disorders such as short bowel syndrome, failure-to-thrive and celiac disease. Dr. Oliva-Hemker has been named among "America's Top Doctors" by Castle Connolly Medical Ltd. for more than 10 years and is among Marquis' "Who's Who in Medicine and Healthcare." She has been named a Baltimore "Top Doc" and has received a U.S. News and World Report "Top Doctor" designation for being in the top 1% of pediatric gastroenterologists in the nation.
Maria Oliva-Hemker, M.D., was named a "top doc" in 2013 in Baltimore Magazine's annual rank list..
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- Cornish Ale
- Sweet nutty malt, zesty orange and spicy hop aromas.
- About this bottle: This bottle contained ale has been uniquely brewed using locally grown Cornish gold malt, traditionally malted using a special kilning process to develop a deep bronze colour and intense flavour. The sweet, lightly nutty malt balances well with zesty orange and spicy aromas from the hops.
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2009 May 14
Chelsea Gets Cosmopolitan
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Mary Katrantzou To Collaborate With Adidas
Mary Katrantzou is entering the athletic wear realm with a new collaboration with Adidas. The London-based designer, who is known for her statement making prints, follows Pharell Williams...
The Outnet Toasts Five Years With An Anniversary Collection Of Exclusives
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Genetic Denim Gets A Boost From Major Investors, Jimmy Iovine and Peter Morton
Genetic Denim is on track to transform from a jeans label into a lifestyle brand, thanks to a successful second round of funding via two big-name investors. Peter Morton, co-founder...
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A reported crowd of 1,700 first cheered when Creighton's name was called in the NCAA tournament selection show. Then it realized the Missouri Valley Conference champions with a 28-5 record somehow slipped to a No. 8 seed in the Midwest Region.
Drawing Alabama was like Jack Lemmon moving in with Walter Matthau. The NCAA's odd couple will create a number of matchup issues both sides with two completely different styles clashing in Greensboro, N.C.
Creighton coach Greg McDermott was surprised his team was assigned such a challenge after winning seven straight games and beating two ranked opponents on the road this year.
"It's really hard to figure," he told reporters in Omaha after the brackets were revealed. "There weren't a lot of differences on paper on what could have been a 5-seed and a possible 8-seed. You really split hairs to try to figure it out."
Either way, his Bluejays will see an Alabama team that looks nothing like the view in the mirror.
Simply put, Creighton is a sharp-shooting team with a porous defense while Alabama hasn't found the touch all season, but defended its way to the tournament.
Led by first-team All-American Doug McDermott, the coaches' son, the Bluejays lead the nation shooting 50.9 percent from the field. Their 3-point percentage (42.5) is only slightly behind Alabama's overall shooting accuracy rate of 45.2 percent. The Tide hasn't even made enough 3s to make the official NCAA statistical rankings, but if it had, the 28.5 shooting percentage would be fourth-worst in the 282 qualifying teams in Division I.
Alabama will counter the third-ranked 3-point offense with the nation's No. 6 perimeter defense that allows just 28.3 percent shooting from behind the arc. Its last loss, however, saw Florida make 10 3-pointers in the SEC quarterfinals when Alabama twice fouled perimeter shooters late in the second half.
Tide coach Anthony Grant said Monday morning he was still gathering information on his opponent, but he's already seen film of the Bluejays' 83-79 win over Illinois State in the conference title game. He saw Creighton make 12 3-pointers in the overtime win while committing just six turnovers. Doug McDermott scored 33 points from all over the floor.
"I'm a little bit familiar with the things they do," Grant said. "As the day goes on, I plan to do a little bit more."
Extended film study will also reveal Creighton's defense lends itself to Alabama's style. Man-to-man coverage is the preferred style for the Bluejays and would be a relief for the Tide offense.
Starting with its first loss of the season to Georgetown in early December, teams threw a zone defense at Alabama. It dared inconsistent 3-point shooters to pull from long range while clogging driving lanes for point guard Trevor Releford.
But Greg McDermott had little to offer in terms of Creighton's experience with that style.
"That isn't exactly our cup of tea," he said. "It's not what we like to do, but it's obvious that the key to being successful against Alabama is making sure you don't turn the ball over and give them easy baskets in that regard and also keep them out of the paint. They're very explosive going to the basket."
Creighton does a good job avoiding turnovers. It hasn't committed more than eight in the past four games and averages 12.3 to rank 68th nationally.
Alabama players JaMychal Green and Andrew Steele said they've seen Creighton play a few times on television. Neither, however, watched with the eye of a future opponent.
"I've watched some of their games and never imagined we'd be facing them but it's a great feeling," Green said. "They are one of the top 25 teams in the nation so it's good to get to play a team that's ranked, get our name out there and compete."


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MATBUS to install technological shelter near MSUMMoorhead, MN (WDAY TV) - No more standing out in the wind and snow while waiting for the buses for students at MSUM.
By: WDAY Staff Reports, WDAY Staff Reports, WDAY
Moorhead, MN (WDAY TV) - No more standing out in the wind and snow while waiting for the buses for students at MSUM.
MATBUS received $100,000 through a special grant to upgrade shelters in Moorhead that were 30 years old.
On 14th street, near MSUM's dormitories, a new $53,000 dollar shelter is going in that will feature a state of the art kiosk, lights and heat.
Lori Van Beek, MATBUS: "Well the kiosk tells you when the bus will arrive in real time. So you are not standing out there ten minutes waiting when you know the bus is still six blocks away. Usually the time is short but you will know when the bus will come. But if you got out there at the wrong time or if you've never ridden before you can see right away when the bus is going to come."
The new shelter should be complete later this month. Two new, but more basic shelters were put up on the 11th street one way near MSUM and another spot near Concordia.
Tags: news, technology, transportation, moorhead, msum, updates


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Sigma-Aldrich S...ntific Seminars
Introduction to Karl Fischer Titration
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Introduction to Karl Fischer Titration
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Andrea Felgner, Hydranal Market Segment Manager
As a leading authority on Karl Fischer Titration, we have extensive experience of product development and customer applications.
Karl Fischer Titration is generally the most accepted method of water content determination and the webinar will provide an introduction to the technique.
May 15 2012
56 mins
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Sigma-Aldrich Scientific Seminars
Practical Utilization of CRISPR in the Laboratory Workflow - Session 2
Apr 22 2014
33 mins
Erika Holroyd, ZFN and CRISPR Technical Scientist
This 40 minute webinar will focus on the practical laboratory techniques needed to create and validate genetically modified cell lines using CRISPR. A Sigma-Aldrich® technology expert will discuss guidelines and techniques utilized when performing genome editing experiments.
Topics will include:
•Transfection of CRISPR reagent into a cell line
•How to validate the activity level of CRISPR
•Clonal isolation
•How to screen clonal populations based on desired modification
Practical Utilization of CRISPR in the Laboratory Workflow - Session 1
Apr 22 2014
30 mins
Erika Holroyd, ZFN and CRISPR Technical Scientist
This 40 minute webinar will focus on the practical laboratory techniques needed to create and validate genetically modified cell lines using CRISPR. A Sigma-Aldrich® technology expert will discuss guidelines and techniques utilized when performing genome editing experiments.
Topics will include:
•Transfection of CRISPR reagent into a cell line
•How to validate the activity level of CRISPR
•Clonal isolation
•How to screen clonal populations based on desired modification
Preventing Culture Shocks: Understanding the Issues of Cell Line Authenticity
Mar 20 2014
54 mins
Jim Cooper, Cell Biology Applications Scientist, ECACC
Despite our dependence on cultured cells as models, issues surrounding cell identity, choice of appropriate cell lines, conditions of their growth and storage, microbial contamination and over-passaging are often overlooked or misunderstood. Mistakes can have devastating impacts on the legitimacy and reproducibility of science but potentially also, on careers, reputations and funding streams.
Topics covered:
Solutions and options for managing cell culture processes
The Impact of misidentification
The international consensus on cell identity and qualification
Genome Editing with CRISPR Systems - Session 2
Jan 14 2014
62 mins
Greg Davis, PhD., R&D Principal Scientist, Gene Regulation Group
CRISPR endonucleases have recently taken the research world by storm with their elegant and simple mode of RNA-guided gene targeting. CRISPR publications have flooded the literature in recent months, verifying that CRISPR endonucleases can operate via protocols developed for ZFN-based genome editing in human cells, rats, flies, worms, and plants (to name only a few).
Genome Editing with CRISPR Systems - Session 1
Jan 14 2014
58 mins
Greg Davis, PhD., R&D Principal Scientist, Gene Regulation Group
CRISPR endonucleases have recently taken the research world by storm with their elegant and simple mode of RNA-guided gene targeting. CRISPR publications have flooded the literature in recent months, verifying that CRISPR endonucleases can operate via protocols developed for ZFN-based genome editing in human cells, rats, flies, worms, and plants (to name only a few).
Proficiency Testing with Microorganism Standards
Dec 2 2013
13 mins
Richard Glindon
A guide to using Vitroids from Sigma Aldrich as a tool for achieving laboratory efficiency
HPLC Method Development and Transfer Utilizing Fused-Core® Particle Technologies
Dec 5 2012
31 mins
David S. Bell, Ph.D.
Superficially porous (Fused-Core, Core-shell) particle technologies have gained acceptance in general high-performance liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) practice over the past several years due to improved efficiency relative to comparably sized fully porous particles. The Fused-Core option has also been shown to be a superior approach toward improving column efficiency as compared to smaller porous particle (sub-2 µm, UHPLC) technologies owing to the lessened backpressure penalties that are paid for the efficiency gains. Ascentis® Express HPLC columns were initially introduced that employed Fused-Core particles with an overall 2.7 µm diameter. These columns provide efficiencies equal to sub-2 µm particles at much lower backpressures as well as superior efficiencies when compared to fully porous 3 µm phases. There are still instances, however, where the backpressure generated by a 2.7 µm particle may preclude their use and there are also situations based on available equipment or regulatory guidance where larger particles are preferred. For these reasons, a new generation of 5 µm Fused-Core columns has been developed.
In this seminar we will provide an introduction to the new line of Ascentis Express 5 µm HPLC columns and explore several scenarios practicing analytical chemists might encounter:
• Desire to develop method on the more efficient 2.7 µm column, but need to be able to transfer to different location or lab
1. Can I easily transfer methods from 2.7 µm to 5 µm?
2. Can I easily transfer methods from 5 µm to 2.7 µm?
• Wish to transfer methods based on fully porous column technologies to Fused-Core
1. Can this be done easily?
2. What gains should I expect? What can be done to optimize the gains?
3. Can I transfer both 3 µm and 5 µm fully porous particle method to Fused-Core?
Evaluations of a New Ionic Liquid Stationary Phase with PEG Like Selectivity
Oct 31 2012
49 mins
Leonard M. Sidisky, Supelco R&D Manager
Geminal dicationic and polyionic ionic liquids have been prepared for use as stationary phases in capillary gas chromatography. These materials are known to provide higher thermal stability for gas chromatography, broader liquid working ranges and broader selectivity ranges than monocationic ionic liquids and polymeric based stationary phases with similar polarity. Recently, a new ionic liquid stationary phase has been developed that provides a selectivity very similar to a polyethylene glycol (PEG) selectivity but with increased thermal stability and lower bleed. We will compare and contrast the similar but unique selectivity of this new phase with traditional PEG phases using a wide variety of different sample types. We will demonstrate the improved thermal stability and lower bleed through a series of different studies and applications.
New! Introducing Ascentis Express 5 um HPLC Columns with Fused-Core Technology
Sep 21 2012
53 mins
R. A Henry
Core-type particles are competing strongly with small porous particles to improve the speed and resolution of HPLC and UHPLC experiments. The pioneering Ascentis Express column with Fused-Core® 2.7µm particles has exploded in popularity because it operates more ruggedly at much lower pressure than current sub-2µm porous particles, yet delivers the same ultra-high performance. This unique performance has been largely attributed to very narrow particle size distribution. Fused-Core® design advantages have also become popular for LC-MS because Ascentis Express columns surpass performance of columns with 3µm porous particles and operate ruggedly at higher velocities and similar pressures.
With new, narrow-distribution 5µm Fused-Core® particles, the same design advantages can now be realized over traditional 5µm and 3µm porous particles that remain very popular for HPLC columns. An Ascentis Express 5µm column brings 3µm performance and extreme ruggedness at 5µm pressures to your laboratory. Like Ascentis Express 2.7µm particles, the 5µm particles show flatter van Deemter plots than same-size porous particles and allow separation speed to be maximized with minimal loss of resolution. Extremely high plates per pressure are observed. The core-type 5µm design should replace porous 5µm columns in routine HPLC applications with traditional instruments, and should also compete with porous 3µm columns in many LC-MS applications. Performance will be compared to 5µm and 3µm particle columns, and examples of method transfer will be shown. Ascentis Express 5µm will be available in the same phase modifications as the original Ascentis Express 2.7µm ultra-high performance column. Highly stable columns are available in various IDs and lengths up to 25cm.
Retention Mechanisms in HILIC Chromatography: Robust Method Development
Jun 28 2012
55 mins
David S. Bell, Ph.D.
Hydrophilic interaction liquid chromatography (HILIC), especially in conjunction with mass spectrometry (MS), has become a powerful tool for the analysis of a wide variety of challenging analytes. Applications of the technique have increased dramatically over the past decade, especially for the analysis of polar analytes where reversed-phase chromatography suffers. HILIC conditions employ a high percentage of acetonitrile which enables facilitated solvent evaporation in LC/MS sources and thus often an increase in analyte response when compared to more aqueous based systems. The increased retention of polar analytes afforded by HILIC provides improved selectivity and decreases the impact of endogenous species, often leading to improved qualitative and quantitative analyses.
Although HILIC has proven useful, it has also been thwarted with complications including difficulties in method development and method robustness.
In this presentation, studies investigating the underlying retention mechanisms dominant in HILIC chromatography are presented and discussed. Along with reversed-partitioning HILIC is well known to exhibit, ion-exchange and the interplay of the dominant mechanisms are unveiled and used to develop a model of overall retention and selectivity. Interactions that operate using different stationary phase chemistries and conditions are presented. The impact of analyte polarity and charge as well as the variations caused by high percentages of organic on these physiochemical parameters are highlighted. Throughout the discussion, examples of use and misuse of HILIC are employed to illustrate these important concepts to build a solid fundamental foundation for efficient and effective use of this powerful technique.
Introduction to Air Monitoring
Jun 19 2012
60 mins
Mr Klaus Buckendahl, European Tactical Marketing Manager, Sample Handling
Whether your laboratory uses passive sampling, or active air sampling, this webinar will run through the basics of air monitoring, giving some advice on the best equipment to use for your laboratories application.
Certified Reference Materials & Proficiency Testing in the 17025 laboratory
May 29 2012
47 mins
Peter Jenks, Reference Materials and PT Business Development Specialist
Traceable reference materials. RTC have been producing environmental laboratory Proficiency Testing programs and certified reference materials for more than 20 years. More than 20,000 PT samples a year are sent to over 2,500 worldwide participants analysing soil, sediments and both waste and drinking water. This webinar will focus on how Sigma-Aldrich-RTC can support your laboratory with its PT and CRM needs.
Ionic liquid GC columns for the quantitation of trans fatty acids in fats & oils
May 16 2012
39 mins
Pierluigi Delmonte, Ph.D - U.S. Food and Drug Administration
The fatty acid composition of a fat or oil is most commonly assessed by gas chromatography, following conversion of the fatty acids methyl esters. The current most refined analytical methods for the quantitation of trans fatty acids rely on the separations provided by long cyanopropyl siloxane capillary columns. The introduction of capillary columns coated with ionic liquids, such as Supelco SLB-IL111, provide an alternative separation tool characterized by a higher stationary phase polarity and selectivity toward geometric and positional isomers of unsaturated fatty acids. The use of these novel capillary columns can provide more refined separations of complex lipid samples. As a result, most conjugated linoleic acid isomers (including t7,c9- and c9,t11-18:1 FA) can be quantitated in a single separation using a 100 m SLB-IL111 capillary column and most 18:1 FA positional and geometric isomers can be separated using a 200 m SLB-IL111. Ionic liquid columns provide more detailed FA profiles, especially for unsaturated fatty acid positional/geometric isomers.
Introduction to Karl Fischer Titration
May 15 2012
62 mins
Andrea Felgner, Hydranal Market Segment Manager
As a leading authority on Karl Fischer Titration, we have extensive experience of product development and customer applications.
Karl Fischer Titration is generally the most accepted method of water content determination and the webinar will provide an introduction to the technique.
Miniaturization of Sample Handling in Bioanalysis with SPME
May 9 2012
50 mins
Professor Janusz Pawliszyn - University of Waterloo
Bio-Solid-phase microextraction (BioSPME) is a simple, fast and sensitive non-exhaustive sample preparation technique that allows the integration of sampling and sample preparation steps. The objective of current research was to automate SPME in 96-well plate format for the first time. The proposed system allows sample preparation of >1000 samples/day, simultaneous determination of both free and total concentration and suitability for performing ligand-receptor binding studies. Open-bed configuration of SPME enables direct handling of heterogeneous matrices such as whole blood, thus further simplifying the entire sample preparation process. The automation of SPME in 96-well plate format enables the highest throughput of any SPME technique to date.
In metabolomics studies of biofluids, the efficiency of metabolism quenching and stability of analytes in selected biofluid dictate how accurately the analytical results represent true metabolome composition at the time of sampling. However, complete quenching of metabolism is not easily accomplished and/or changes due to poorly stable compounds can occur, so the processes of sampling and sample preparation can significantly affect metabolome's composition. The use of SPME for direct in vivo sampling of drugs and metabolites in the bloodstream of freely moving animals eliminates the need for blood withdrawal in order to generate pharmacokinetic (PK) or metabolomic profiles in support of pharmaceutical drug discovery studies. This is particularly important for situations with a limited blood volume such as mice because it enables the use of a single animal to construct an entire profile or conduct longitudinal studies. The aim of the current research was to apply SPME for in vivo sampling in mice for the first time. Furthermore, we investigate the use of in vivo SPME as an effective sample preparation method for both targeted pharmacokinetic and untargeted LC-MS metabolomics studies.
Introduction to SPME (Solid Phase MicroExtraction)
Apr 24 2012
69 mins
Klaus Buckendahl
SPME (Solid Phase MicroExtraction) is a solventless extraction technique, used to remove and preconcentrate low levels of volatile and semivolatile organic compounds from soil, water and air.
This webcast will cover the basics of SPME; how to get started, and suggested applications of the technology.
Expanding the Capabilities of GC and MS with Ionic Liquids
Mar 21 2012
41 mins
Prof. Daniel W. Armstrong
Ionic liquids (ILs) and their components have had a substantial impact on the capabilities and performance of gas chromatography, ESI-MS and MALDI-MS. For GC this ranges from the analysis of complex mixtures by GC x GC, water quantitation and food/beverage analysis among other areas. In MS the most sensitive analysis of anions, phospholipids and metal cations is often done by ESI-MS using complex IL cationic reagents. Also the best MALDI matrices for many proteins, peptides, carbohydrates and synthetic polymers are based on ILs. These will be discussed as time permits.
Introducing Ascentis Express ES-Cyano HPLC Columns
Nov 30 2011
37 mins
David S. Bell, Ph.D. - Analytical Research & Development Manager, Supelco
10 minute introduction to the Ascentis Express family with an emphasis on the benefits of the new ES-Cyano phase. The presentation will then feature a 20 minute advanced level presentation on the molecular interactions contributing to alternative retention and selectivity using Cyano stationary phases
Using LC/MS to meet the challenges of a high through-put clinical diagnostic lab
Sep 21 2011
42 mins
Michael JP Wright, Ph.D. Cambridge University Hospital Trust
"With a BSc in Biochemistry and Molecular Biology from the University of Western Australia, I started my postgraduate career in the world of cell signaling and transgenic research at the Laboratories of Cancer Medicine at Royal Perth Hospital. After a number of years I relocated to the UK and started working in clinical diagnostic laboratories where I had a change of direction in my career. Moving into the development of HPLC and LC-MS/MS methods for clinical diagnostic analysis, I plied my trade at Guys Hospital and King's College Hospital's in London for 6 years before taking my current post at Addenbrooke's hospital in Cambridge in late 2009. My work interests include new born screening, biogenic amines, steroids and vitamin analysis in an array of human sample types."
Analysis of Contaminants & Competition: Handling R&D Roadblocks
Jun 15 2011
29 mins
Aaron Cassely, VP-Technology, Chemir Analytical Services
Analytical R&D teams are thought of as a core facility within a company. On top of their regular R&D duties, they are often asked to solve production problems, ranging from identifying a contaminant to investigating a competitor's new product. Learning how to approach these challenges and considering the use of resources beyond your laboratory can save both time and money.
Three case studies will be presented. The case studies involve method development for sub-ppm detection, identifying an off-odor in food, and the detection of a low level pigment. A focus on the specific analytical technology will be presented in the case studies.
Focusing on new / innovative technologies and industry challenges
The Sigma-Aldrich seminar series features scientific presentations from key specialists in analytical chemistry, chemistry and life sciences, on the practical and technical aspects of new developments and innovations, to help advance your research.
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While attending the University of New Haven in the 1980s, David Peterson took to the UNH gridiron as a defensive end. Today, he maintains his love of football but now focuses his talents on pursuing another passion: private enterprise.
Dave has owned and operated a number of successful businesses over the past two decades, ranging from sports entertainment restaurants to printing businesses to credit card platforms.
He currently is President and CEO of 4 Media Online, a credit card technology and consulting business with locations in Florida, Los Angeles, Toronto and Spain. The company insures maximum client profitability and competitive advantage, by virtue of the firm's technological expertise and superior fraud detection. Dave also is a co-founder and partner of PowerBoatNation.com, an online destination for all things power boating.
An Edgewater, N.J., native now living with his wife, Karen, and their two daughters, Nicole and Mackenzie, in Coral Springs, Fla., Dave is fervent about giving back to the community and prides himself on aªliations with organizations which make an impact. He is actively involved with Soldiers' Angels, an organization that provides aid and comfort to the men and women of the U.S. Army, Marines, Navy, Air Force, Coast Guard and their families.
He also is a member of Indian Princess Seminole Nation, an organization dedicated to providing services that enrich the father and daughter bond, and with Soles4Souls, which distributes shoes to people in need.
A member of UNH's Board of Governors, Dave organizes the University's yearly alumni football trip to Miami, which brings together former Charger football players for three days of networking and socializing. The event, which includes a Miami Dolphins football game, generates support for the University of New Haven football program.
Dave credits his education and his involvement with sports at UNH for instilling many qualities in him: the ability to adapt to an ever-changing environment, juggle challenges, set goals and map a plan for success, as well as discipline, teamwork and respect.
"My UNH coaches and professors were positive role models who were fundamental in shaping who I am today," he says. "The UNH experience provided me with a degree and the tools to help achieve my ultimate goal of running a successful business worldwide. I am proud to be part of the UNH team."
Posted Spring 2012