text:
Location: Agricultural Systems Research2010 Annual Report
1a. Objectives (from AD-416)
1. Develop strategies for irrigated production systems using increased crop diversity, reduced tillage, and emerging technologies to improve agricultural chemical, water and nutrient use efficiencies. 2. Develop biological based disease control strategies for NGP production systems. 3. Develop dryland production strategies with increased crop diversity and advanced technologies to improve agricultural chemical, water, and nutrient use efficiencies to increase competitiveness and enhance natural resource quality.
1b. Approach (from AD-416)
This project involves eight scientists (8 SYs) and many collaborators that represent a broad range of disciplines focused on the long-term, 10- to 12-year goal of developing sustainable strategies for both irrigated and dryland crop production systems for the MonDak region of eastern Montana and western North Dakota. The ASRU is organized to address basic and applied research issues using an interdisciplinary team approach where multiple hypotheses are tested in four large "Unit Projects." The "Unit Project" objectives are designed to encompass cropping system development, concurrent development and application of "metrics" or indicators of system performance, and the assessment process by which systems are evaluated and judged. There is considerable overlap, but responsibilities can be generally divided into two broad, overlapping categories with four scientists (Allen, Evans, Lartey and Stevens) primarily examining the systems aspects, and four scientists (Caesar-TonThat, Jabro, Lenssen and Sainju) focusing their research on developing and evaluating various metrics of cropping system performance. These Unit projects focus on biologically diverse cropping systems. Biological control research (Obj. 2) of two plant diseases spans both dryland (Obj. 3) and irrigated (Obj. 1) systems. Three of the Unit projects (one irrigated and two dryland) are currently underway and one new irrigated project will be initiated in 2009. Each Unit project will be replaced by a new Unit project as they are completed.
3. Progress Report
Remote sensing techniques were used for the second year in relation to post-harvest plant residue assessment with collaborators in northeastern Montana; Nesson Valley, ND (Northern Great Plains); Akron, CO (Central Great Plains); Pendleton, OR and Pullman, WA (Pacific Northwest) on malting barley, spring wheat, peas, durum and fallow. A follow-up field data collection was also conducted in spring before planting season in the MonDak region. The cellulose absorption index (CAI) value was shown to readily distinguish fields practicing conservation tillage from those that are not. Soil, plant, and greenhouse gas samples were collected and analyzed and microbial enzymatic activities were completed for the two GRACEnet sites. Work progressed toward determining best management practices for N fertilization for strip till sugarbeet and understanding C cycling in MonDak region cropping systems. A study evaluating the effects of strip tillage systems and high-efficiency irrigation methods on 2-year sugarbeet and malt barley rotations was completed along with an accompanying study to evaluate the effects of strip tillage system on N availability and uptake. Field work was completed for two studies designed to determine optimum fertilizer management for sugarbeet grown on sandy textured soils. A study was continued evaluating polymer-coated slow-release urea as means to improve sugarbeet root yield and reduce N use on sandy soils. Data collection commenced on a new, long term irrigated cropping systems project that includes sugarbeet, legumes, barley and/or bioenergy crops. A four year field study was started to evaluate the effects of freezing and thawing cycles on changing soil physical properties of severely compacted clay soils over time. Collaborative evaluations at dryland and irrigated research sites using the RZWQ2 model were continued. Progress was made in many areas on five different dryland cropping rotation experiments. Selected treatments were replanted during the establishment phase of two perennial biomass trials that includes grasses with varying levels of N fertility compared to binary grass-legume mixtures. The 3rd year was completed of a 2-year spring wheat rotation with oilseeds (camelina, crambe and B. juncea) that replace summer fallow. Determination of the aggregate distributions plus isolation and storage of dominant bacteria from soil aggregates or from rhizosphere samples were completed for several irrigated and dryland studies. Yield and yield components were measured in a camelina investigation varying seeding depths and seeding rates. ASRU scientists developed a primer set for real-time PCR detection of P. teres (Net Blotch disease of barley and wheat). Antibodies for ELISA based detection of both P. teres and Laetisaria arvalis have been developed and tested. Investigations were continued on various L. arvalis treatments as an alternative to fungicide seed treatments for barley. In collaboration with the BLM, a new investigation was started to evaluate the effects of Agaricus spp of basidiomycete fungi (responsible for fairy rings) on communities of native grasses and soil aggregation in rangelands of Eastern Montana.
4. Accomplishments
1. Affordable, reliable remote sensing technique verified for use in ag computer models. Increasingly sophisticated computer models allow for assessments of the impacts of soil, water and energy conservation and new production methods on the sustainability of various cropping systems. But these assessments are only as good as the tools used to measure the many variables involved. Crop residue cover, a key indicator of soil carbon storage and soil conservation benefits, is one such variable that has previously been highly labor intensive and expensive to accurately calculate. To address that issue, ARS scientists at Sidney, MT in collaboration with researchers at the ARS Hydrology and Remote Sensing Laboratory, Beltsville, MD, examined use of the remotely-sensed cellulose absorption index (CAI) to evaluate crop residue cover and the subsequent sustainability of various cropping systems in semi-arid regions. Measurements were made in 2009 and 2010 in the Northern Great Plains (Sidney, MT and Akron, CO) and Pacific Northwest (Pendleton, OR and Pullman, WA) regions using a ground-based multispectral camera, digital photography and transect lines on research plots and grower fields to assess post harvest residue measurements of different dryland cropping systems. Scientists have determined that CAI can be used to provide reliable estimates of crop residue cover in these semi-arid dryland regions, which can, in turn, be utilized in computer models to help determine the sustainability of different cropping systems in these regions. In addition, in collaboration with scientists at the Livestock and Range Research Laboratory in Miles City, MT. the technique has also been shown to successfully assess residue cover and subsequent "fuel loads" for computer models looking at wildfire potential of rangelands. The technique also holds potential for many other computer model applications looking at carbon sequestration and to aid compliance with government farm conservation programs.
2. Legumes and annual forage crop rotations enhance desirable soil properties for sustainable dryland yields in the semi-arid Northern Great Plains. Maintaining adequate levels of soil water and soil nitrogen (N) are critical for sustainable dryland crop yields in semi-arid regions. Also, nitrogen fertilization is a significant expense for producers. The introduction of alternative crop rotations incorporating nitrogen-rich legumes holds promise for improved sustainability and profitability in the region. However, the time, expense and expertise needed to evaluate new cropping rotations and their impacts prevent interested producers from exploring or adopting these options on their own. Consequently, Sidney, MT ARS scientists initiated a four-year study of two dryland rotations comparing continuous alfalfa and no-till durum-annual forage sequences and assessing their impacts on soil nitrogen and water levels. They found that inclusion of perennial and annual legume forages in rotation with cereal crops can help sustain soil nitrogen and water levels by supplying nitrogen and reducing soil water uptake. The study showed that alfalfa increased soil carbon and nitrogen storage and nitrogen mineralization at the surface layer due to increased root biomass, and reduced water and nitrate-N content in surface and subsurface layers. Nitrogen mineralization and availability were greater in durum-Austrian winter pea/barley hay than in the other durum-annual forage sequences studied. Overall, the research showed that growers can reduce production costs (i.e. N fertilization amounts) while maintaining crop yields through incorporation of legume forages such as Austrian winter pea hay in rotation with cereal crops. The research also shows that production of dryland alfalfa can reduce N leaching and increase soil carbon and available soil N. Soil carbon sequestration also provides climate change benefits and may also benefit producers economically under carbon trading programs.
3. Annual forages identified as profitable alternatives to summer fallow in semi-arid dryland farming operations. Economic forces are driving the replacement of wheat-summer fallow cropping systems by diversified, continuous (every year) cropping systems in dryland crop production areas of the semi-arid Northern Great Plains, but results are quite variable and growers lack direction. ARS scientists at Sidney, MT have determined that annual spring-seeded forage crops use less water than cereal grains, including durum, and may be a suitable replacement option to summer fallow. A five year experiment was completed that compared yield, quality, and water and nitrogen use of durum-fallow rotations with two-year rotations of continuous durum and three annual forages: forage barley, forage barley interseeded with field pea, and foxtail millet. Averaged over years, preplant soil water and residual nitrogen content were greater for durum following fallow than for durum following annual forages, resulting in reduced total fertilizer N requirement and greater yield, water use, grain N accumulation and nitrogen recovery index (NRI) following fallow. In addition, replacing summer fallow with annual forages reduced durum grain yield by 10.8 bushel acre-1. But while those factors appear to favor the wheat-fallow system, forage yields of nearly 2.5 ton acre-1 produced a higher annualized return over the five-year study period for all three wheat-forage rotations, greatly reduced herbicide use and substantially lowered total fertilization costs, despite reduced wheat yields. Under the study, the annualized net returns in the three annual forage-durum systems were $51 acre-1, $31 and $14 acre-1 greater than for fallow-durum, respectively.
4. Minimal tillage practices shown to substantially reduce sugarbeet production costs. Sugarbeet growers in the Northern Great Plains are looking for ways to reduce the cost of energy, labor and time invested in farming while increasing profits. Tillage practices are a major expense, but different approaches may influence physical, chemical, and biological properties of the soil environment in different ways and, thereby, crop yield and quality. A 4-yr field study was conducted by ARS scientists at Sidney, MT comparing three tillage practices: no tillage; strip tillage (10-cm deep) and deep tillage with chisel plow of (30-cm deep) on a sandy loam soil planted to sugarbeet at the North Dakota State University irrigated research farm near Williston, ND. Deep tillage represents the conventional method used in the region, and it typically requires larger equipment, more time and more fuel to complete as opposed to no tillage or strip tillage methods. The data showed that strip tillage had no significant effect on root yield, sucrose content, or sucrose yield, and, unlike no tillage, sugarbeet population emergence was not significantly impacted. In the latter instance, the data showed that sugarbeet emergence was significantly lower in no tillage than in either strip tillage or deep tillage for all dates throughout the growing season. This research demonstrated that farmers could substantially reduce tillage costs using minimal tillage practices (i.e. strip tillage) with little significant effects on sugarbeet yield or quality. As a result of this study and related studies at the Montana USDA lab, sugar beet producers in the Northern Great Plains and in other regions have begun incorporating strip tillage in their operations in ever increasing numbers because of the economic benefits demonstrated.
5. Identification of production practices that improve spring wheat and field pea yield and nitrogen use in semi-arid environments. Primary constraints to dryland crop production in the Northern Great Plains are available soil water and nitrogen (N) fertility; however, these factors may be either positively or adversely influenced by crop rotation, tillage, and management practices. In an effort to better understand and predict these influences, ARS researchers at Sidney, MT initiated a unique long-term, multi-disciplinary study examining several diverse, multi-year crop rotations using varied tillage and conventional and ecological management practices. In their preliminary results, ARS researchers found that diversification beyond a two-year rotation did not improve spring wheat yield, although field pea appears to yield consistently higher when planted every third or fourth year compared to every other year. Researchers also found that conventional tillage systems by themselves did not impact crop yield compared to no till, and that ecological management practices increased yields of pea, but decreased yields of spring wheat, although the latter is largely attributable to the later planting date used under the ecological system, as opposed to other ecological practices. To date, results from this long-term study suggest that an increased level of rotation diversification with wheat and pea (annual legume) rotations and ecological management can improve total yield, in part through increased N use efficiency. This further indicates that reduced costs associated with no tillage and decreased economic and environmental risk associated with no-till practices can improve N use efficiency and increase economic returns to producers. Ultimately, researchers hope to provide the region's producers with several different cropping strategies for increasing net returns while maximizes environmental benefits as a result of this study.
6. Malt barley-field pea rotation lower greenhouse gas emissions and reduced nitrogen fertilization. Maintaining sustainable crop yields and lower carbon dioxide emissions with respect to nitrogen (N) fertilizer rates are major concerns in irrigated and dryland cropping systems in the Northern Great Plains. Increased N fertilization can boost yields, but also plays a major role in various greenhouse gas emissions, which makes finding the proper balance important for producers and the environment. Two large studies by ARS scientists at Sidney, MT have demonstrated that a no-tilled malt barley-field pea rotation may be an economically viable alternative for producers hoping to strike that balance. Results from a three year dryland and a five year irrigated-dryland study comparing the effects of tillage, cropping sequence, and N fertilization in northeastern Montana and northwestern North Dakota have shown that no-till malt barley-pea rotations reduced N fertilization rates by 20 kg N ha-1 and increased annualized malt barley yield compared with conventionally tilled malt barley-fallow cropping systems. Other findings show tillage increased carbon dioxide (CO2) flux compared with no-tillage in irrigated cropping systems, but had no effect on crop yield and CO2 flux in the corresponding dryland cropping systems. Continuous, every year cropping also increased total crop returns and CO2 fluxes compared with fallow in dryland cropping systems. Similarly, N fertilization increased crop yield, but reduced CO2 flux compared with no N fertilization in both irrigated and dryland systems. These studies show that better selection of cropping systems and tillage can reduce both CO2 emissions and the need for purchased N fertilizers for both dryland and irrigated cropping systems. This study is part of a nationwide ARS effort designed to provide producers with strategies for reducing agriculture's greenhouse gas emissions and provide a scientific base for carbon trading programs.
7. Best nitrogen fertilizer management practices identified for strip till sugarbeet production. The profitability of many farms producing major commodity crops is declining because of escalating costs of energy, fertilizers, and other purchased inputs. Strip tillage of sugarbeet is receiving wide spread attention because of the potential to substantially reduce energy and equipment costs for producers. ARS researchers at Sidney, MT have shown that while N availability and uptake tend to be somewhat lower with strip tillage than with conventional tillage practices, sugarbeet yield can be maintained at comparable levels with the same N application rates used for conventional tillage systems as long as proper placement and application timing practices are followed. This research has demonstrated that guidelines for N fertilization of conventional tillage sugarbeet can successfully be applied to strip till sugarbeet production. This finding provides important information regarding N management for sugarbeet grown with strip tillage systems where fertilizer is banded within the tilled strip. It also saves agronomists the time and expense that would otherwise be required to develop new fertilizer recommendations specifically for strip tillage.
8. Sheep grazing can control weeds and increase nutrient cycling in soils to sustain crop yields. Wheat and livestock producers are looking for ways to reduce herbicide and feed costs, respectively. The largest use of glyphosate herbicides in Montana is for control of volunteer wheat during noncrop, fallow periods. In collaboration with Montana State University, ARS scientists at Sidney, MT conducted a five-year study to evaluate and compare the effects of sheep grazing with chemical fallow and tilled fallow management systems on soil properties, weed community, and spring wheat yields. Results document that grazing slightly reduced soil carbon, and available nitrogen, phosphorus, and potassium contents, along with pH, cation exchange capacity, and electrical conductivity by reducing the amount of crop residue returned to the soil compared with tilled and no-tilled treatments. Grazing, however, increased soil calcium, magnesium, and sodium contents. Grazing had no effect on wheat grain and biomass yields, but reduced the need for herbicides by reducing populations of some problem weed species. The study demonstrated that properly timed sheep grazing can be an effective way to economically control volunteer wheat and other weeds and sustain crop yields by reducing selection pressure for weed resistance to glyphosate, decreasing potential leaching of nitrates, and increasing nutrient cycling in soils.
9. Increased host range of Turnip vein clearing virus determined. Since discovery of Arabidopsis thaliana as a host of Turnip vein clearing virus (TVCV), scientists have used the plant as a model for studying virus transmissions in plants. Recently, new hosts of TVCV have been identified by an ARS scientist at Sidney, MT in collaboration with colleagues at the University South Carolina at Columbia and Sumter. This work confirmed the long held hypothesis that TVCV had a broad host range, and the identification of new hosts enables additional studies on how a single virus interacts with different hosts in the process of causing infection. The discovery is a major step forward in the crucial understanding of several mechanisms in host-pathogen interactions including intercellular movement of viruses, long distance movement of viruses within a host, and virus transmission across hosts. This increased understanding is ultimately expected to aid in developing management practices limiting virus transmission in plants such as methods that could curb seed transmission of plant pathogenic viruses either through breeding or emerging molecular techniques. This increased understanding also has implications well beyond plant systems. Understanding the mechanisms of virus transmissions in plants, not only provides opportunities to (combat/limit) those transmissions in crops, but may also provide additional insights regarding virus transmissions in animals and humans.
10. Long-term soil aggregation in semi-arid dryland systems is aided by a no-till wheat-lentil rotations. Soil aggregate formation and stability are key indicators of soil health, which, in turn, is a key factor in sustaining crop yields and quality, increasing soil water storage and reducing erosion, particularly in semi-arid regions like the Northern Great Plains. Soil aggregation is significantly impacted by common farming practices, including tillage and the types and sequences of crops grown, but until recently those effects have been not been quantified. ARS researchers at Sidney, MT developed a set of biological markers specifically to study the long-term tillage and cropping effects on soil aggregation and aggregate stability in dryland areas of the semi-arid eastern Montana area. In this study, researchers used those markers to compare soil aggregation levels in long-term spring-wheat systems using varied tillage and alternating crops, along with continuous wheat and wheat-fallow. Results have conclusively shown that: 1) aggregate stability improved over 12 years of no-till continuous spring wheat systems compared to wheat-fallow rotation with the values approaching those of samples from nearby perennial grass fields, and 2) long-term, continuous wheat-lentil rotations did not differ in aggregate stability compared to a wheat-fallow rotation. However, leaving lentil residues on the soil surface to control erosion was shown to minimize N mineralization making more N available to the subsequent crop, thereby reducing economic and environmental risks to the region's producers. Consequently, leaving moderate amounts of lentil residue on the soil surface in no-till wheat-lentil rotations was found to be a better alternative for farmers in eastern Montana interested in long term sustainability of their operations than fallow or tilled lentil-wheat systems.
11. Collaborations expand crop diversity in the northwestern U.S. and boost rural economic development. A major agronomic concern in the Northern Great Plains region is the limited diversity of crops grown by producers, especially in dryland areas. That limited diversity increases risk to producers, contributes to disease and pest problems and impedes rural economic growth by limiting options to producers and small businesses. Consequently, ARS scientists at Sidney, MT are participating in two large, regional collaborative research initiatives with the intention of increasing dryland cropping diversity for the region's producers and promoting rural economic development in these areas. Partners include the National Pulse Crop Association, several major universities, producers, seed companies, biofuel refiners, and various major end users of biofuels including airports and transportation providers. The first initiative is evaluating sustainable cropping systems, incorporating various dryland pulse crops produced in the Pacific Northwest and the Northern Great Plains regions. Researchers are also looking at the human health benefits associated with these same pulse crops. The second initiative enhances collaborative efforts with a consortium of universities, industry and ARS researchers evaluating sustainable cropping systems incorporating various oilseed crops produced across the region to ensure continuity of jet fuel supplies for commercial and defense aviation and other uses. In addition to their widespread regional focus, these initiatives also address cropping diversity and economic development issues raised by stakeholders in several local focus group meetings in eastern Montana and western North Dakota, where the vast majority (80%) of the nation's annual pulse and oilseed crops are grown.
5. Significant Activities that Support Special Target Populations
A Native American Intern from South Dakota who is attending the United Tribes Technical College in Bismarck, ND had an 8 week internship with the Agricultural Systems Research Unit in Sidney, MT in 2010. The intern assisted with research on remote sensing of agricultural plant residues as part of a national ARS multi-location project. He initiated a small, personal research project on determination of plant residues (specifically, cheat grass and yellow clover) in rangelands. Overall, the internship was quite positive for both the student and ARS.
Review Publications
Jabro, J.D., Stevens, W.B., Iversen, W.M., Evans, R.G. 2010. Tillage depth effects on soil physical properties, sugarbeet yield and quality. Communications in Soil Science and Plant Analysis. 41: 7:908 -916.


text:
GM Names Vehicle Safety Chief in Wake of Recall
General Motors CEO Mary Barra recently talked with reporters in a roundtable meeting to discuss GM's handling of last month's recall of 1.6 million vehicles.
Barra discussed when she knew of the ignition switch problem as well as GM's efforts to prevent future safety problems.
Barra Unaware of Problem
Reuters reports that Barra was unaware of the ignition switch defect while she was head of GM global product development.
Further, she alleges that she wasn't told of the problem until January 31, two weeks after she was named CEO. Barra did, however, say that she was told in late December of product review of the Chevrolet Cobalt.
After learning of the defective ignition switches and recall, Barra says she immediately contacted the GM board.
GM Names Vehicle Safety Chief
GM announced today that Jeff Boyer will be the new vehicle safety chief – a new position created as a result of the recall.
Boyer, a 40-year veteran with GM, has been given "complete authority" to make safety changes or changes to the recall process. Boyer is expected to improve GM's recall process and keep Barra briefed on progress.
In the past, Boyer worked on the interiors of some of the recalled vehicles. He was not responsible for any mechanical or electrical components such as the ignition switch.
No Word to Victims' Families
There have been 12 fatalities linked by GM to the defective ignition switches. So far, GM has not reached out to any of the victims' families.
Barra told reporters that GM was waiting to conclude a probe, which she expects to take a "few months," before reaching out.
Safety advocates have also urged GM to set up a $1 billion trust fund for crash victims. Barra declined to comment directly on whether or not that was an action GM plans to take.
$1.8 MillionExpenses: $20,000.00 | Attorneys Fees: $765,000.00 | Net to Client: $1 Million
$2 MillionExpenses: $78,475.96 | Attorneys Fees: $850,087.96 | Net to Client: $1,071,436.00
$2.3 MillionExpenses: $200,000.00 | Attorneys Fees: $900,000.00 | Net to Client: $1.2 Million

subset: commoncrawl


text:
Telephone | Mailing Address | Email
Do not forget to include your vehicle type, year, make and model. Please do not send more than one Email per subject to the appropriate address. To avoid lost Emails and information, we ask that you preset your SPAM-Blocking Programs to accept Emails from mossmotors.com - failure to do so may result in lost communication.


text:
Images of Singapore
Website: www.sentosa.com.sg
Address: 40 Imbiah Road, Sentosa Island Singapore 099700
Getting there:
Take the Sentosa Express into Sentosa and tranfer to either the Red or Blue Line Bus and alight at the Imbiah Lookout Bus Stop.
Contact: (+65) 6279 3284
Operating Hours:
Daily from 9am - 7pm (Last entry 6.30pm).
Tales of Singapore's history are brought to life in this award-winning attraction with multimedia displays, theatre presentations and life-sized exhibits. Images of Singapore promises a journey to the origins of the nation with legends, facts and folklore weaved into a story from the early days to the present. The various intricately created scenes allow you to place yourself there to feel a part of history. Before you leave, take a piece of history with you to remember these moments with a variety of unique collectibles and books from the Images of Singapore shop.
View Fullscreen Map
Search ComeSingapore for articles, events and directories matching your keywords.


text:
Targeting the right end users with transmission solutions designed specifically for their applications will strongly enhance the prospects for product sales. Custom-made designs will gain popularity as well.If you are interested in more information on this study, please send an e-mail with your contact details to Julia Nikishkina, Corporate Communications, at email@example.com. European Mechanical Power Transmissions Market is part of the Mechanical Power Transmission Growth Partnership Service programme, which also includes research in the following markets: Motor Management and Control Devices, Advanced Motion Controllers Market in Europe and, European Market for Integrated Motors and Drives. All research included in subscriptions provide detailed market opportunities and industry trends that have been evaluated following extensive interviews with market participants. About Frost & Sullivan Frost & Sullivan, the Growth Partnership Company, works in collaboration with clients to leverage visionary innovation that addresses the global challenges and related growth opportunities that will make or break today's market participants. Our "Growth Partnership" supports clients by addressing these opportunities and incorporating two key elements driving visionary innovation: The Integrated Value Proposition and The Partnership Infrastructure.
- The Integrated Value Proposition provides support to our clients throughout all phases of their journey to visionary innovation including: research, analysis, strategy, vision, innovation and implementation.
- The Partnership Infrastructure is entirely unique as it constructs the foundation upon which visionary innovation becomes possible. This includes our 360 degree research, comprehensive industry coverage, career best practices as well as our global footprint of more than 40 offices.


text:
Save This Whale
cute, eco-friendly, unique, and handcrafted!
A unique little accessory for the home. This mason jar stores sunlight, and casts a subtle glow later in the night.Added 7 Years Ago from Amazon.com
Any gal who goes barefoot knows that a gravel path is the pits! This is great. And, if you choose to change your lawn decor... hey just roll it on up! Gives new meaning to when the rubber hits the road! : )Added 10 Years Ago from Rubbersidewalks
Cute, wearable and better than being in a hotdog costume!


text:
Bai Ling cranks up moonstruck life for "High Voltage"
By Alex Dobuzinskis
LOS ANGELES (Reuters) - Chinese-American actress Bai Ling really thinks she is from the moon, and that her grandmother lives there. Really, truly.
The actress, who stars in the action movie "Crank High Voltage" that opened on Friday, has made the admission before, and she insists she is not crazy -- just inspired.
"I'm not really in reality. I'm in my own universe and my mind is a million miles somewhere else," said Bai, who won a Golden Horse award, a Chinese-language film honor, for her supporting role in 2004 movie "Dumplings."
"Why I feel like I come from the moon is because my mother told me I was found somewhere," she said, explaining that at night she often sees the grandmother who raised her in the celestial sphere.
During a stay at a mental hospital in China as a teenager, after a difficult spell in the army, Bai said she told the staff that she was of sound mind.
"I went to the nurse, I said, 'Nurse I'm not crazy. I'm an actress, I want to get out of here, I'm doing a movie,'" she told Reuters in a recent interview.
As improbable as it may have seemed at the time, Bai truly was a budding actress. Soon after her release from the hospital, she was cast in a stage play, then went on to win roles in a number of Chinese movies in the 1980s.
So, as impossible as it may seem that she's from the moon and no matter what some moviegoers may say about her sometimes odd fashion choices, she is, nonetheless, a solid actress. Continued...


text:
I was a student nurse at Lord Mayor Treloars from January 1962 until January 1964. Those two years orthopaedics were affiliated with other hospitals which allowed us to start training before 18 which was the minimum age for General Nursing commencement, when the students moved on the 3 year general training was reduced to two years.
The hospital was a very happy place because many of the patients were long stay, young, cheerful and perfect!
The nurses home is shown in this photograph, it was always warm and friendly. I had several rooms in the home, the attic window just visible beyond the second A was my favourite, the only disadvantage being if you got in from a date late, after 10pm lock up you could not get in a window, then you had to knock up a friend whose room was on the ground floor, she would then pass her laundry box out the window for you to stand on, then you had to lean out and pull the basket back in. Once or twice I was caught but the night sister unlocked the front door and did not report me. Bless her cotton socks! This was a very happy time for me, I loved everything about the hospital, the work, the nurses home, the friends I made, (several of whom I at still in contact with )but most of all the patients, many of them long stay children who were admitted for months.
I notice Nurse Johns has a special mention, I think this will have been Frankie Johns whose first Ward was on 5, with Sister Smith. Ward 4 was the teenage boys ward and believe me the nurses had as much pleasure looking after the boys who were great teasers as the lads had with the nurses. On one occasion a lad named Terry asked for a bottle on a Sunday when the ward was packed solid with visitors, there was only visiting once a week on a Sunday. Being a very new girl, it was my first Sunday visiting duty, I went and got the bottle, pulled the curtains around his bed and passed him the bottle. Terry was on a full plaster bed. A mould of his body had been made in plaster of Paris, this was cut in half. Part of each day would be spent laying on the top half, on his stomach, most of the day in the lower half on his back.
As I passed Terry the bottle he grabbed my wrist and yelled at the top of his voice "Oh! Oh! Nurse Nurse" Well imagine how red my face was when I scuttled out from the closed curtains. I left the curtains and hid in the ward kitchen until visiting was over.
I remember Michael who struggled with legs affected by polio. I remember Andrew who had Downs and lived on the ward all the time I was working on it.
I live near Salisbury now and remember my time in Alton, at Treloars, with great joy.
Bronwen Lovering, formerly Student Nurse Shawyer
A memory shared byon Oct 19th, 2010.
Sun Jul 31st 2016, at 2:36 pm
You must be signed-in to your Frith account to post a comment.
Not sure what to write? It's easy - just think of an important place in your life and ask yourself:
Some of the places you've shared memories of this week:
...and hundreds more! Enjoy browsing more recent contributions now.


text:
The American Man in Europe: Mending Fences, the U.S. Farmer, Iraq and the Rise of China
Traders Magazine, January 2005
Dean LeBaron, techie, quant, futurist, international investor, author. A full introduction of the founder of Batterymarch Financial, would fill pages. He's retired, but still very active. Dean splits his time now between a lake home in Switzerland and one in New Hampshire, where I caught up with him recently only a couple weeks after his return from Europe. Attitudes towards the U.S. there,and in the rest of world were still very much on Dean's mind. His analysis, sobering.
All TradersMagazine.com articles are archived after 7 days. REGISTER NOW for unlimited access to all recently archived articles, as well as thousands of searchable stories. Registered Members also gain access to exclusive industry white paper downloads, web seminars, online technology directory, the iPad App, and conference discounts. Qualified members may also choose to receive our free monthly magazine and any of our e-newsletters covering the latest breaking news, opinions from industry leaders, developing trends and trading strategies.


text:
Diversity in Public Interest Panel
Date:Friday, February 7, 2014 - 12:25pm - 1:30pm
Location:Room I
This Diversity Month program features a panel of minority attorneys discussing their work in public interest and the reasons less minority student pursue work in public interest fields. Organized by Asian Pacific American Law Students Association, Black Law Students Association, Latino/a Law Students Association, Public Interest Law Society, and South Asian Law Students Association. Sponsored by the Office of the Dean of Students.


text:
Belgian actor wins plaudits and first Cannes "crush"
By Mike Collett-White
CANNES, France (Reuters) - It is only day two, but the Cannes film festival has already uncovered a breakout star in Belgian Matthias Schoenaerts, who has earned critical acclaim and plenty of swoons for his portrayal of tough guy Ali in French drama "Rust and Bone".
Schoenaerts is not new to the big screen -- he has appeared in more than 20 films including "Bullhead", nominated for a foreign language film Academy Award earlier this year.
But for thousands of journalists and critics in Cannes for the annual cinema showcase, a press screening on Thursday of the moving love story Rust and Bone ("De Rouille et d'Os") was their first look at the physically imposing 34-year-old.
Within minutes of the movie ending, Schoenaerts was being asked about his prospects in Hollywood.
"It's funny you say so, because last week they called me for 'Rambo 34', and I said I'll do it if I get 35 and 36 as well," he joked at a press conference.
"There's definitely stuff moving in the States, but I've got time, I'm not in a hurry. I'm quite young I guess."
He may hope to replicate the success of French actor Jean Dujardin, who was little known outside France until the Cannes world premiere last year of "The Artist" which set him on a path to global fame and a best actor Oscar.
AVOIDING MELODRAMA Continued...


text:
Hisar, May 28 (UNI) A court here sent two persons, including a woman, into police custody till June 11 for ''sale'' of an Assamese woman as bride for Rs 55,000.
The accused 'groom' Baljeet Singh, and one of the seller Meena have been booked in a case registered under sections 366, 368, 376 and 120-B, for sale of Jonmani Hazarika of village Ghila Moda of Lakhimpura in Assam, Ishwar Singh, incharge of Multani Chowk police post told media persons today.
He claimed that the accused had accepted their crime and the police was further investigating as to how many other girls have been sold to 'bachelors' of Haryana so far.
Efforts were on to nab Meena's husband Omar Ali, who is absconding, he said.
According to the complaint of Jonmani, mother of a five-year-old daughter, she had come here for work and had stayed with Omar Ali and Meena, an acquaintance from her home-land, for about 15 days at local Surya Nagar.
In the name of providing her work as maid, she was actually sold to Baljeet Singh for Rs 50,000 without her knowledge. She is now pregnanat with the child of Baljeet Singh.
Due to the skewed male-female sex ratio in the state, there was an increase in number of eligible bachelors, who in desperation, were opting for purchasing 'wife' from other states through organised groups operating in the state for quite some time, he said.


text:
Mumbai, Aug 8 (UNI) The Walt Disney Company has completed an investment of Rs 1.18 billion in UTV Global Broadcasting Limited (UGBL) for a 15 per cent stake.
UGBL has allocated 3,00,000 equity shares to The Walt Disney Company (Southeast Asia) Ptv Ltd ''Disney'', under the deal struck in February this year.
At the same time UTV Software Communications Ltd (UTV), had been allotted 15,00,000 equity shares of Rs 10 each of UGBL, constituting 75 per cent of the paid up share capital of the company, for an aggregate consideration of Rs 40 billion, making UGBL a subsidiary of UTV.
The remaining 10 per cent continues to be held by Ronnie Screwvala's Promoter Group.
UGBL is the parent company for its two wholly owned subsidiaries that have launched four channels in six months between September last year and March this year.
With the completion of this investment, the largest media and entertainment foreign investments into India, Disney has completed 32.1 per cent acquisition in parent UTV Software Communications Ltd and 15 per cent in the broadcast arm UGBL at a total out lay of Rs 81.83 billion.


text:
My Response
am i less because i only speak english
am i less authentic, less worthy of community, if i only have ~one word for love~
But I feel invisible strings tying me to people halfway around the world. And though I forget how you are related to me, I care about you so much. And I think so often about how I'm not going to get to see my only baby cousins growing up because we're separated by an ocean. I remember being so, so happy when I knew they were going to be born - finally, cousins on my mother's side! I can only hope that my few days with them two years ago leaves some good memory; maybe the oldest will remember me next time we see each other.And though I haven't spoken to my paternal grandmother in ages and ages, I think about my grandparents often and their life and and and. And I insist we go to Tapioca Express after coming back from Universal Studios at night because we weren't able to take my little brother with us so we should at least get him something. And I wish I could go to my cousin, older than me by only one day; I wish I could ask her what went wrong. And I will spend dollars and time and take trains and buses and even airplanes for amazing people in my life. I have missed trains and waited at stations and taken bus adventures. I will meet you halfway. I have held a best friend's hand after she cried during Adoration.
But is that not good enough? Is that not what love is?
Do I really only have one word for love
Is my language thin and fragile and boring and disgusting because it's English? Or do I make it beautiful and heartwrenching and wonderful because in my mouth and out of my fingertips it becomes mine. It becomes the way I feel and share and express my love and pain and desire. Do I really only have one word for love in my English? But isn't love what I talked about in that large paragraph, without once using the ~only word for love~ in my language? (For it is my language because I speak it. Because I can mold it. It is mine.) Are my feelings any less because I cannot express them adequately enough in my first tongue? If so, I guess the constant tears are part of my penance.


text:
Get updated on our latest
styles and other news.
Creating an account is easy and allows you to:
We're here to help
Monday to Friday
9 AM to 8 PM EST
Saturday and Sunday
9 AM to 5 PM EST
Find out about our return policy
Track your order
Frequently asked questions
Melanie Lyne's carefully selected, hand-picked collection of well-known labels offers superior craftsmanship, intricate patterns and designs, as well as fashion-forward styles.
Sign up
to receive
Everyday free shipping
on orders over $150
Free in-store returns
Secure payments.
Shop with confidence
Style rewards program.
Earn points with every purchase
Get updated on our latest
styles and other news.


text:
Glenn from Morganton, NC writes:
I am not familiar with all the new technology, especially as it relates to smart phones. I see offers to download apps on my computer, but have not done so because I think they don't apply to computers, only smart phones, iPads and such. Am I right?
Glenn, an app (short for application) could be for a smartphone, tablet or computer. App really just means program. But to work on your computer, an app must be designed for your operating system.
For example, let's look at the page for WPS Office, a free office suite. You"ll notice that it is available for Android, iOS, Windows & Linux.
If you own an Android tablet or phone, you would choose Android. If you have an iPad or iPhone, you select iOS and for a Windows computer, select the Windows option.
If we look at the page for Instagram, you'll see that there are options for going to the Apple App Store and for Google Play. That means this program can only be used on Apple Devices or Android phones or tablets and is not available for your PC.
Now, if you are using Windows 8 or 8.1, there's an entire app store full off applications designed for your computer. Either search for Store in the search charm or tap the icon that looks like a shopping bag.
Then you can browse the Windows for both free and paid applications for your 8 or 8.1 PC.
You won't find an app that works for your PC in the Google Play Store or the Apple Store. You can find apps in the Windows Store if you have 8 or 8.1.
If you have a Windows 7 PC, you can still find apps that will work with your system. You just need to check to make sure that particular app is compatible with your operating system.
~ Cynthia


text: The hotel has a total of 328 rooms including 10 Rooftop Balcony Suites which overlook the Marina Bay Area. The hotel has 2 separate blocks, mainly the Tower and Podium block, which is linked via a bridge on the 4th floor. The hotel is well protected by 24- hour security guards with modern CCTV system. The hotel is wired with high speed broadband and is Wi-Fi enabled for most open area. Hotel guests can also relax and lounge or surf the internet by the poolside which is located on the 8th floor.|The hotel has a 24 hour reception counter which caters to key collection, check-in/check-out and any other enquires. Car park facilities are available at the hotel.All guest rooms and lifts are equipped with card system access. Some of the in-room facilities include LCD TV, individually controlled air-conditioning, tea & coffee making facilities and high speed internet (chargeable).


text:
Green spent most of his day at the beautiful facility of Hofstra University. His skills were utilized tonight by his high school team -- St. Mary's instead of his AAU team, which was competing at the Rumble in the Bronx tonight.
Green's team was successful once but failed in its latter match. St. Mary's features several division one players, including Green. The high major athlete scored 16 points in his first game tonight against Uniondale. However, St. Mary's finished the evening on a sour note when they were dropped by a deep, young, and very talented Christ The King High School.
Green, who is being recruited by several high major programs, recently took a visit to St. John's University. "I took it on Thursday night," Green said this evening. "It was a great trip.
"I learned a lot about the school and the program. I got to meet the coaches. I got to hear about the new facilities that will be going in there. So it was a great trip."
Green has also expressed a desire to visit Connecticut as well. He also lists North Carolina State, Georgetown, Virginia, Pittsburgh, and Florida. "I am going to make my decision in the fall," Green stated. "I want to take some official visits before I decide. But I will try to take some unofficial visits too."
Green, who will play again at the Team Hofstra camp tomorrow morning, may also join the New York Panthers later in the day to compete at the Rumble in the Bronx at Fordham University.


text:
|The Confederation Train; photo by Jim Brown.
Forty-six years ago, in 1967, a special train crossed Canada--the Confederation Train.
The train, made up of two FP9A locomotives (numbers 1867 and 1967) and eight colourful cars, was a travelling exhibit about Canadian history.
The lead locomotive had the Centennial symbol emblazoned on its nose under the headlight, and its horn sounded the first four notes of O Canada. (Hear it here)
The train made its public debut in Victoria, B.C. on January 9, 1967. It arrived in Atlantic Canada in October, and ended its journey in Montreal in December of that year.
During its tour, it stopped at 60 cities and towns, and was visited hundreds of thousands of Canadians.
|In Swift Current, Sask.; Scott Dunsire collection.
Modeller Fred Barkhouse was so enamoured of the train that he decided to make a model of it--something he describes in the latest issue of Canadian Railway Modeller.
Fred's inspiration for the project came from a Lionel HO scale train set called the Confederation Flyer that was owned by his father. The train set had a locomotive, ten 50-foot boxcars and a caboose; each boxcar was painted for a province with their flag and the date they joined Canada.
|Lionel's Confederation Flyer.
Since Fred made his train before InterMountain brought out its units in Confederation Train colours, he used Highliner F9 shells on Athearn Genesis drives for the project. The cars were made from Rapido Super Continental passenger cars.
(Eleven years later, in 1978, the National Museum of Canada created the Discovery Train, which crossed Canada for two years as a mobile museum. That train showcased the landscape of Canada from the Atlantic to the Pacific.)
|The 1978-80 Discovery Train.
2017 is the 150th birthday for Canada. I wonder if there will even be a passenger train left on the rails in this country to make into a similar travelling exhibit?
Read all about Fred's great model, including how he made it, and see more photos in the latest issue of CRM. See videos of Fred's Confederation Train on YouTube here and here. Read more about the prototype train and see more photos here.


text:
Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea
- First Online:
- Received:
- Accepted:
- Cite this article as:
- Rosanas-Urgell, A., Mueller, D., Betuela, I. et al. Malar J (2010) 9: 361. doi:10.1186/1475-2875-9-361
- Citations
- 7.9k Downloads
Accurate diagnosis of Plasmodium infections is essential for malaria morbidity and mortality reduction in tropical areas. Despite great advantages of light microscopy (LM) for malaria diagnosis, its limited sensitivity is a critical shortfall for epidemiological studies. Robust molecular diagnostics tools are thus needed.
The present study describes the development of a duplex quantitative real time PCR (qPCR) assay, which specifically detects and quantifies the four human Plasmodium species. Performance of this method was compared to PCR-ligase detection reaction-fluorescent microsphere assay (PCR_LDR_FMA), nested PCR (nPCR) and LM, using field samples collected from 452 children one to five years of age from the Sepik area in Papua New Guinea. Agreement between diagnostic methods was calcualted using kappa statistics.
The agreement of qPCR with other molecular diagnostic methods was substantial for the detection of P. falciparum, but was moderate for the detection of P. vivax, P. malariae and P. ovale. P. falciparum and P. vivax prevalence by qPCR was 40.9% and 65.7% respectively. This compares to 43.8% and 73.2% by nPCR and 47.1% and 67.5% by PCR_LDR_FMA. P. malariae and P. ovale prevalence was 4.7% and 7.3% by qPCR, 3.3% and 3.8% by nPCR, and 7.7% and 4.4% by PCR_LDR_FMA. Prevalence by LM was lower for all four species, being 25.4% for P. falciparum, 54.9% for P. vivax, 2.4% for P. malariae and 0.0% for P. ovale. The quantification by qPCR closely correlated with microscopic quantification for P. falciparum and P. vivax samples (R2 = 0.825 and R2 = 0.505, respectively). The low prevalence of P. malariae and P. ovale did not permit a solid comparative analysis of quantification for these species.
The qPCR assay developed proved optimal for detection of all four Plasmodium species. Densities by LM were well reflected in quantification results by qPCR, whereby congruence was better for P. falciparum than for P. vivax. This likely is a consequence of the generally lower P. vivax densities. Easy performance of the qPCR assay, a less laborious workflow and reduced risk of contamination, together with reduced costs per sample through reduced reaction volume, opens the possibility to implement qPCR in endemic settings as a suitable diagnostic tool for large epidemiological studies.
Rapid and accurate diagnosis of Plasmodium infections is crucial for morbidity and mortality reduction in tropical areas, specially in regions where mixed infections are prevalent such as Papua New Guinea (PNG), where all four parasites infecting humans coexist and mixed species infections are common [1, 2]. For improving accuracy in large epidemiological studies, molecular diagnostic tools permitting high through put analysis for the identification and quantification of malaria parasites would be of great benefit.
Traditionally, light microscopy (LM) examination of blood smears has been considered the gold standard for the diagnosis of malaria [3]. LM has clear advantages over molecular typing, since it incurs only low costs, does neither need complex sample preparation nor advanced technology and permits species identification and quantification [4]. However, the role of LM as a gold standard has been questioned due to false negative results at low parasitemia and frequent errors in species identification in mixed infections [5, 6].
The availability of commercial rapid diagnostic tests (RDT) has greatly facilitated the in situ diagnosis of malaria infections in the field. The advantages of RDT are that they do not need special preparation of the sample and diagnostic results become immediately available [7]. However, their use is limited due to lack of sensitivity for Plasmodium vivax, Plasmodium malariae and Plasmodium ovale[8].
Nested PCR (nPCR), first described by Snounou and co-workers [9], is a widely used method and is considered as a molecular gold standard due to its good performance in detection of mixed species infection. This assay amplifies the multicopy 18 S rRNA genes of the four Plasmodium species infecting humans. Even though this genotyping technique is now performed in many field laboratories in endemic countries, its use for routine clinical diagnosis is limited, because the analysis is time consuming due to the need of multiple reactions per sample, and the risk of contamination through the requirement for nPCR [9]. Moreover, the technique is not quantitative.
In recent years, various real time quantitative PCR (qPCR) assays have been developed for the detection of Plasmodium species, with most assays targeting the 18 S rRNA genes. Two of these methods detect the genus Plasmodium using generic primers, and thus do not distinguish between species [10, 11]. Sybergreen reagent has been used by other groups, to identify the four Plasmodium species infecting humans in a single reaction by melting curve analysis [8, 12]. The use of TaqMan probes contributed an additional level of specificity to qPCR assays [13]. However, using a single pair of primers for Plasmodium genus detection in duplex assays, introduces competition for amplification among species, which likely leads to difficulties in detecting mixed infections [14]. In order to address this issue, various groups had used different strategies [15, 16, 17, 18]. The multiplex PCR-ligase detection reaction-fluorescent microsphere assay (PCR_LDR_FMA) has also been used in molecular epidemiological studies for simultaneously detecting all four Plasmodium species [19].
Despite the variety of molecular tools available for the diagnosis of malaria and their wide use for the diagnosis of imported malaria in travel clinics, implementation of these techniques in endemic areas has remained limited until now. Even though the low sensitivity and limited detection of asymptomatic and mixed infections by LM constitutes a critical shortfall for some epidemiological studies, LM remains to date the most frequently used method for the diagnosis of malaria in endemic areas. The transfer of molecular techniques for diagnosis of malaria to laboratories in endemic settings is essential for overcoming the limitations by LM. Moreover, a molecular technique with quantification capacity contributes to correctly estimate the burden of Plasmodium species often found in concomitant infections and will be a valuable tool to explore competition in mixed infections.
A qPCR assay initially developed for malaria diagnosis in returning travellers at a reference laboratory was implemented and validated at the PNG Institute of Medical Research (IMR). This assay was chosen because it detects with high specificity all four Plasmodium species which jointly occur in our study area in PNG. The performance of this qPCR assay in conditions of a field laboratory and on field samples was compared to light microscopy, nPCR and PCR_LDR_FMA results.
Study site and sample collection
Samples were collected in 10 villages from Ilahita area of East Sepik Province, PNG [2], in the framework of a longitudinal cohort study conducted during 2008. As part of the baseline, venous blood samples were obtained from 452 children one to five years of age, after written informed consent was obtained from parents or legal guardians of each child. This genotyping study was approved by the PNG IMR Institutional Review Board (IMR IRB 0720) and PNG Medical Research Advisory Committee (MRAC 07.34).
Blood smear examination
Thick/thin blood smears were prepared as described previously [20, 21]. Blood smears were stained with a 5% Giemsa solution and examined independently by two microscopists, with a third microscopist reading for those slides with discrepant results. A minimum of 200 microscopic fields were examined at a magnification of 1000× using oil immersion optics before a slide was declared negative for malaria parasites by LM. Plasmodium species were quantified by counting infected parasites over 200 leukocytes. Conversion of parasite counts into parasites/μL was performed assuming a mean leukocyte count of 8000/μL whole blood. Routinely slides were read twice. Discordant results were evaluated by a third slide reading. Final species diagnosis was based on the majority agreement.
DNA template extraction and amplification
DNA was extracted from 200 μL whole blood (venous blood collected in EDTA anti-coagulant) using QIAamp 96 DNA Blood Mini Kit (QIAGEN, Valencia, CA), and eluted in a final volume of 200 μL dH2O according to the supplier's instructions. nPCR was carried out as described [22].
PCR_LDR_FMA was carried out as described elsewhere [19]. Mixes of serially diluted plasmids containing inserts of P. falciparum, P. vivax, P. malariae or P. ovale 18 S rDNA were used as positive controls in addition to P. falciparum or P. vivax positive samples obtained from field isolates. The threshold for positivity for each species was determined using the mean value obtained from negative controls for each species, plus three times the standard deviation.
Primers and probes for qPCR
Sequence 5'-3'
Fluorescent label
P. falciparum
P. falciparum
P. falciparum
P. vivax
P. vivax
P. vivax
P. ovale
P. ovale
P. ovale
qPCR validation
Evaluation of PCR efficiency and reproducibility was performed on standard curves using four positive control plasmids with the respective amplicons inserted. Geometric mean and standard deviation were calculated from triplicates in three independent assays. Standard curve for each Plasmodium species was made from a 10-fold serial dilution of the control plasmids ranging from 106 copies/μL to 10 copies/μL.
Amplification efficiencies for the different primer pairs and probes were calculated with the formula:
Efficiency = 10(-1/Slope) -1. Inter-assay coefficients of variation (CVs) were calculated for each plasmid dilution separately as (SD/mean)*100 using the Ct values from different runs.
Reproducibility of qPCR was further analysed by repeating 10% of all 452 field-samples in the same laboratory, but at different time points. External quality assurance was performed in addition.
The amount of target in an unknown sample was quantified by converting the threshold cycle (Ct) into template copy number by using the four standard curves. Samples yielding Ct values equal or higher than 40 were considered Plasmodium species negative [23].
Statistical analysis
Results from qPCR was compared with those from LM, nPCR and PCR-LDR-FMA. Agreement between diagnostic methods was determined by calculating Kappa Statistics with 95% confidence intervals. Values were interpreted with the Landis and Koch classification [24] as follows: k = 0.41-0.60, moderate agreement, k = 0.61-0.80, good agreement; k = 0.81-1.00, almost perfect agreement beyond chance. Prevalence for each species was calculated by dividing the number of positive samples through by the number of all samples tested and McNemar test was calculated in order to test for significance. P values <0.05 were considered statistically significant. Correlation between LM and qPCR quantification was calculated by pairwise correlation coefficient. All statistical calculations were performed with STATAv.10 statistical software.
Optimization of qPCR assay
The large scale of field work required optimization of molecular testing with the aim to reduce costs. Two strategies were applied: multiplexing the qPCR and reducing the reaction volume. The performance of the two duplex qPCRs were evaluated by analysing dilution series of the two respective control plasmids in various ratios (Additional file 1). These experiments demonstrated very little inhibition when 200 templates and above were present in the reaction. Only the detection of minute template concentrations, as low as 20 templates per reaction, was slightly inhibited by excess amounts of the alternative template, i.e. in a 100 times excess.
qPCR Efficiencies for each Plasmodium species
Efficiency %
R squared
P. falciparum
P. vivax
P. malariae
P. ovale
Reproducibility of our assay was investigated by re-analysing 10% of the 452 samples in the same laboratory. In 8/45 compared samples discordant results were recorded. Four of these discrepancies occurred in cases of low density in the range of 10-100 copies/μL. Ct values were between 37 and 39 in the positive ones of the duplicate tests. Two other discrepancies were observed in mixed species infections, whereby the minority species was detected in only one of the duplicate experiments in the range of 10 to 100 copies/μL.
Comparison of diagnosis of Plasmodium infection by qPCR, nPCR, PCR-LDR and LM
P. falciparum versus P. vivax agreement among different diagnostic techniques
0.750 substantial
0.621 substantial
0.553 moderatee
0.609 substantial
0.611 substantial
P. malariae versus P. ovale agreement among different diagnostic techniques
Quantification of Plasmodium parasites
In preparation of major molecular epidemiological field studies in PNG essential parasite detection techniques were compared under conditions of a laboratory close to the field site and located in a malaria endemic country. The diagnostic requirements were: (i) good performance in the detection of mixed species infections, as all four species concurrently occur in PNG, (ii) recognition of P. malariae and P. ovale variants present in the study area, (iii) high through put capacity and robustness of assay, (iv) quantitative results and (v) reasonable costs. The qPCR assay described here was implemented and validated at the PNG-IMR site in Madang, demonstrating the feasibility of applying state of the art techniques in this context. In the meantime the qPCR assay is routinely implemented for molecular diagnosis in large scale epidemiologic studies at IMR.
As part of test validation in the field, the performance of this qPCR assay for Plasmodium species discrimination was compared to two other PCR-based assays (nPCR and LDR) and to LM. Traditionally, test outcomes for different assays are compared to an established 'gold-standard' in order to calculate sensitivity and specificity estimates and to evaluate the performance of newly developed tests. The classical 'gold standard' for malaria diagnosis has been LM [3], however, with the appearance of new molecular diagnostic tools, LM has become less suitable for this purpose due to its lower sensitivity than molecular methods [6]. Even though the nPCR developed by Snounou et al [9] has been extensively used as 'gold-standard' for molecular diagnosis [25, 26], the concept of using a 'gold-standard' for the evaluation of new assays is being questioned by various authors, which alternatively propose the use of 'non-gold standard' approaches [27, 28].
The agreement between qPCR and the other techniques was substantial for P. falciparum, but only moderate for P. vivax, P. malariae and P. ovale. In particular, the agreement between qPCR and nPCR for P. falciparum detection was almost perfect. The lower agreement between PCR-LDR and nPCR, together with the higher prevalence shown by PCR-LDR (47.1% compared to 40.9% by qPCR and 43.8% by nPCR), may indicate false positive results by LDR. This is supported by our pairwise analysis and the agreement of two independent PCR based assays, namely nPCR and qPCR. However, in absence of a suitable diagnostic 'gold standard', it remains unclear if those 33 samples positive for LDR but negative by the two alternative molecular methods, represent a greater sensitivity of LDR or simply false positives. This issue cannot be easily resolved in a study involving 'unknown' samples from the field, potentially infected by four different Plasmodium species.
P. vivax prevalence was higher by nPCR than by both, qPCR and PCR-LDR (73.2% by nPCR, 65.7% by qPCR and 67.5% by PCR-LDR). This again could reflect false postitives by nPCR or lower sensitivity by both other molecular methods. Our observations in qPCR validation using plasmid template suggested that qPCR of P. vivax is lightly compromised by performing a duplex Pf/Pv reaction. nPCR involves a very high number of cycles (55 cycles by nPCR versus 45 cycles by qPCR and 35 cycles by LDR), and therefore is expected to show maximal sensitivity. Despite measurements taken over 45 cycles in qPCR, we followed the consensus rule for considering a sample positive, i.e. a Ct value < 40 [23]. In our samples this led to the loss of 9 samples with Ct values for P. vivax between 40 and 43.6 cycles, which otherwise would have increased the sensitivity of the assay. Further analysis was performed on samples with discrepant results for P. vivax (P. vivax negative samples by qPCR and positive by nPCR). Most of these samples were mixed infections by nPCR and harboured P. falciparum with more than 10,000 copies/μl. Thus competition for amplification at the beginning of the PCR due to P. falciparum high densities may be precluding P. vivax detection [14]. 14/16 of the remaining samples were also negative by LM. Most likely these very low-grade P. vivax infections were missed. The scarcity of the template in case of a very low parasite density is expected to lead to imperfect detection. Prevalence for P. malariae and P. ovale were low with significant differences between assays, even though the agreement between pairwise compaired methods was moderate. Higher prevalence for P. malariae detection by LDR is likely to occur as a result of false positive results, probably occurring due to high background noise of the P. malariae probe used in the assay. Low detection of P. ovale by nPCR (3.8%) is due to the use of a primer pair with sub-optimal amplification of P. ovale sequences present in the study area. Finally, LM measured the lowest prevalence for all four Plasmodium species.
The major advantage of qPCR over the other compared molecular techniques was the quantification of parasite densities. Parasite densities shown as copies of 18 S rRNA template/μL were quantified by converting the threshold cycle (Ct) into template copy number by using the standard curves. When correlating quantification by qPCR with LM counts in samples where both techniques showed positive results, a high correlation for P. falciparum (R2 = 0.8253) and a lower correlation for P. vivax (R2 = 0.5049) was found. But for P. vivax this correlation of parasite densities by qPCR and LM increased when only single infections were taken into account. Therefore, our results suggest a variation in the detection limit in both methods, due to overlooking P. vivax in case of an overwhelming P. falciparum infection. Difficulties in identifying P. vivax by LM arise when this parasite is found at low densities and in mixed infections. The high P. falciparum densities found in the samples identified as mixed infection by qPCR (> 10000 target copies/μl) further supports this explanation. The correlation for P. malariae and P. ovale could not be analysed due to poor detection of both species by LM.
The qPCR assay was found optimal for both tasks, detection of all four Plasmodium species and quantification. The latter could only be analyzed for P. falciparum and P. vivax. Overall qPCR shows substantial agreement with other molecular techniques for the detecting prevalence of P. falciparum and P. vivax, while moderate agreement was observed for P. malariae and P. ovale. It is clear, that sensitivity of our qPCR assay can be increased by simply performing independent reactions of each Plasmodium species. However, this would substantially increase costs. Limiting factors, such as duplex assays, need to be balanced against costs or work load. The specific research objectives of a particular study should guide the choice of experimental procedures.
Overall, the superior performance of PCR based methodologies over LM has been clearly demonstrated by these results and others. In a recent study conducted in Benin, a high number of children (between 27% and 44%) aged 5 or above, who initially had negative RDT tests (most also with negative blood slides), were later found to be infected with P. falciparum using PCR [29]. These undetected submicroscopic infections have an enormous impact for malaria transmission in endemic areas. In a time where malaria erradication has become the primary goal of malaria agendas, the accurate estimation of the burden of malaria infection is imperative to control transmission.
In conclusion, this qPCR assay was sensitive and specific for the detection of all four Plasmodium species and results agreed well with other molecular techniques tested. Added advantage of the qPCR assay is quantification of parasite densities and a less laborious workflow. Moreover, the assay performed well in field samples and due to its high through put capacity, it is suitable for large scale epidemiological studies. Finally, a quantitative assay is of a greatest value for monitoring of malaria control programmes.
We are particularly thankful to all the children of Ilahita area, their parents and/or guardians who participated in the study. We thank the staff from the Institute of Medical Research in Maprik for their assistance in collecting and processing blood samples, to the staff from the Institute of Medical Research in Madang for malaria microscopy readings and to Nomin-Dora Tenakanai for her help with the Bioplex analysis. This investigation received financial support from the Fundación Privada CELLEX (Catalonia, Spain).
Supplementary material
Copyright information
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


text:
Christ Is Our Life (7 of 12) by Johnny Hunt
This content is part of a series.Christ Is Our Life (7 of 12)
Johnny Hunt
Colossians 3:15-17
October 19, 2003
INTRODUCTION: In Col. 3:13-17 Paul outlines four spiritual motivations for godly living. It is so evident that the centrality of this living is Jesus Christ. He is the centerpiece of Christianity.
1. We forgive because Christ forgave us. 13
2. It is the peace of Christ that should rule in our hearts. 15
3. The Word of Christ should dwell in us richly. 16
4. The name of Christ should be our identification and our authority.
(Col. 3:11, "Christ is all, and in all"
The Scriptures remind us that since Christ is our life, we have all the resources needed for godly living. However, we must be spiritually motivated in four areas:
1. To Live for Him
2. To Obey Him
3. To Honor Him
4. To Glorify Him
Because we have experienced the grace of God, we want to live for Him. Because we have enjoyed the peace of God, we want to obey Him. Because we have been enriched by the Word of God and enabled by the name of God, we want to honor and Glorify Him.
This will all happen:
"Peace of God"
Romans 5:1, "Therefore, having been justified by faith, we have peace with God through our Lord Jesus Christ."
EXAMPLE: Billy Graham's Tract, "Peace with God"
The war between the unbeliever and God is over and the treaty was paid at the cross.
Ephesians 2:14, "He Himself is our peace, who has made both one, and has broken down the middle wall of separation."
"He Himself" – emphatically emphasizes that Christ
alone is the believer's source of peace.
"Peace of God"
Isaiah 26:3, "You will keep him in perfect peace, whose mind is stayed on You, because he trusts in You."
Philippians 4:6-7, "Be anxious for nothing, but in everything by prayer and supplication, with thanksgiving, let your requests be made known to God; and the peace ...
There are 9893 characters in the full content. This excerpt only shows a 2000 character sample of the full content.
Price: $4.99 or 1 credit
Sign up for a Free Trial with SermonSearch.com and download this sermon free today!
Featured Worship Backgrounds


text:
Product Catalogue
Forever Sun Lips
3.5 out of 5 stars based on 2 user ratings.
Take care of your lips by applying SPF 30 protection that blocks both UVA and UVB rays. This is a real must-have product for the summer months and offers perfect lip protection during ski season. It's water-resistant for up to eighty minutes and has a refreshing cool-mint flavour.
Weight: 0.15oz
Retail: £4.26, €5.12
Not available
Retail: £4.26, €5.12
Not available
Apply to the lips regularly, particularly during the winter and summer months.
Avobenzone, homosalate, octisalate, octocrylene, petrolatum, glycine soja (soybean) oil, ozokerite, butyloctyl salicylate, flavour, beeswax, ethylhexyl hydroxystearate, euphorbia cerifera (candelilla) wax, simmondsia chinensis (jojoba) seed oil, copernicia cerifera (carnauba) wax, menthol, menthyl lactate, aloe barbadensis leaf extract, allantoin.
No available ingredients for this product.


text:
HDX HDXFWS Fine Wire Stapler (Reconditioned)
- Description
DescriptionThe HDX Pneumatic Fine Wire Stapler fastens Arrow Brand T50 staples in sizes ranging from 1/4 in. to 9/16 in. The die-cast aluminum housing and high quality internal components are durable while still remaining lightweight, and the hardened steel driver blade is built to last. The easy-load magazine holds up to 100 staples at a time. The adjustable exhaust, non-marring tip, and comfortable grip allow for a user-friendly experience.
- Features
FeaturesComfortable grip Die-cast aluminum construction Anti-dust cap Oil and wrenches included Non-marring tip helps protect your work surface from any scratches or marring inflicted by the tool
- Specifications
SpecificationsAir Tool Type - Air Nailer
Assembled Depth (in.) - 2.25 in
Assembled Height (in.)- 7 in
Assembled Width (in.) - 9 in
Cordless/Corded - Cordless
Energy Type - Pneumatic
Loading type - Strip
Maximum Fastener Size - 9/16 in
Maximum fastener size - 0.5625 in
Minimum Fastener Size - 1/4 in
Minimum fastener size - 0.25 in
- Includes
- Manuals
- Videos
Product Video
- Reviews
Customer Reviews 6 item(s)
- Big Sky Tool Reviews
Looks and works like new, Great value.
Quality Value Price
- Big Sky Tool Reviews
It is perfect . Thanks
Quality Value Price
- Big Sky Tool Reviews
This T-50 wire staple gun is great for these most common staples that we used to have to use hammer tacker/stapler or wear out my arm squeezing off staples with a spring loaded Arrow brand staple gun. Now I can work without wearing out my arm. If you consider the cost and work savings, this stapler is the bargain of the year. Great idea!
Quality Value Price
- Big Sky Tool Reviews
It is a great little stapler for the money.
Quality Value Price
- Big Sky Tool Reviews
Very pleased with this stapler so far. Works as expected and has certainly saved my hand from the torture of using a manual staple gun.
Quality Value Price


text:
Search Sathorael's books
Random books from Sathorael's library
Gilead's Blood (Warhammer) by Dan Abnett
Descent of Angels (The Horus Heresy) by Mitchell Scanlon
The Devil's Right Hand (Dante Valentine, Book 3) by Lilith Saintcrow
Horus Rising (The Horus Heresy) by Dan Abnett
The Four Forges: The Elven Ways #1 (Elven Ways) by Jenna Rhodes
Fire Warrior (Warhammer 40,000) by Simon Spurrier
Darkblade: Bloodstorm (Warhammer) by Dan Abnett
Members with Sathorael's books
RSS feeds
Reviews of Sathorael's books, not including Sathorael's
Helper badges
Site design selection
The old design is no longer fully supported nor does it get full attention when we roll out new features. We strongly recommend using the new design.
Member: Sathorael
CollectionsYour library (48), Currently reading (1), All collections (48)
TagsCurrently Reading (1) — see all tags
Cloudstag cloud, author cloud, tag mirror
About meI Cook at a Japanese Resturant called Miyako in Greenville, I have too many habbies, just to name some of them in general; 40K, Warhammer, Battlefleet Gothic, Hordes, Tai Chi Quian Yang Style, Karatedo, SCA, Archery, Hiking, Weights, Video/PC/PS3 Games, Reading, Otaku, Languges and Cultures(Mostly- Japanese, Korean, Thai, Mandarin Chinese), and Random Research...
Favorite authorsDan Abnett, R.A.V. Salsitz, Mercedes Lackey, Sandy Mitchell, Ian Watson (Shared favorites)
LocationTaylors, SC
Account typepublic, lifetime
/profile/Sathorael (profile)
Member sinceApr 1, 2008
Currently readingDead Man Rising (Dante Valentine, Book 2) by Lilith Saintcrow
Most recent activity


text:
Obsessed with technology?
Subscribe to the latest tech news as well as exciting promotions from us and our partners!
By subscribing, you indicate that you have read & understood the SPH's Privacy Policy and PDPA Statement.
Driven with SandForce's SF2281 controller and SK Hynix, the Strontium 120GB Hawk SSD delivers read/write speeds of 510/470MBps.
|SSD Capacity
|Flash Memory Type
|SandForce 2nd Gen SSD SF-2281VB1-SDC Processor
|Advertised Sequential Read/Write Speeds
|Advertised Random Read/Write Speeds
|SSD Format
A night of Exclusive Promotions, Free Goodie Bag & Vouchers and more!
Enjoy up to $1,100 trade-in discount with the all-new Galaxy Tab S3!
Vote now for the best user review & stand a chance to win Sony speaker
Dual 20MP Cameras. Beauty Made Clearer.
Close shave without nicks and cuts. Compare full range promo here
5 ways the LG G6 makes you want to take lots of photos.
Take control, even in challenging conditions, with the agile D7500


text:
Ferrari is celebrating the fact that its Facebook page has passed eight million fans a few days ago. Well, it doesn't take much to understand why. The brand is the top player in the automotive sector and is the most famous one in its class. To thank its fans, Ferrari made a special video of the 458 Italia. The figure 8,000,000 was drawn on the steering pad at the Fiorano race track, which was dedicated to all of the fans.
Ferrari also holds the No. 1 position at Google network G+. It has more than half a million fans. This number has increased by ten times in the first quarter of 2012.
At Weibo (one of the most relevant social network in China), the Ferrari brand became the most popular brand in its segment even when it hasn't been long since it entered the site. Ferrari has proven just how popular its road cars, as well as racing cars, are around the world. Ferrari's fans aren't concentrated in traditional markets like the USA, Great Britain, Italy and Spain, but so many of them are in emerging countries like India and Brazil, even as far as Maranello, Tonga, Samoa, and Vanuatu, as well as the Afghanistan and Libya, and Iran.
If you want to say how much you love (or hate) Ferrari, you could air it out on any of its four different Twitter accounts -@insideferrari for the activities of the Scuderia, @ferrariraces for the Ferrari Challenge single-marque championship, @insideFDA for the Ferrari Driver Academy and @ferrarimagazine for the official magazine. The official Ferrari channels prove how sincere the brand is to have direct contact with its fans everywhere.


text:
Dhaka, Mar 11: India captain MS Dhoni urged his young guns not to put themselves under pressure to copy batting legends like Sachin Tendulkar and retired Rahul Dravid.
Dravid, on Friday, announced his retirement from international cricket making way for several youngsters such as Virat Kohli, Suresh Raina and Rohit Sharma to stake a claim for his number three slot.
"Every individual is different and anyone who comes in the side should not even think of replacing the kind of seniors that we have got," Dhoni told reporters on the eve of the four-nation Asia Cup.
"The youngsters will (put themselves) under tremendous pressure if they start to think like that. They are legends of the game, enjoyed the sport and played with the right kind of spirit throughout.
"Anil (Kumble), Rahul, Sachin, (Vangipurappu) Laxman, Sourav (Ganguly), it's tough to replace them so there is no point thinking you have players who will replace them."
Dravid showed pure class during the tour of England but failed to perform during the tour Down Under. Dravid, Tendulkar and VVS Laxman have scored over 45,000 Test runs but failed to score a single century between them.
Virat Kohli managed to be the only centurion during the Australia tour, but in-form Rohit Sharma was sidelined and had to wait for chance to prove himself during the Tests series.
"Every individual is special, he brings special character into the side so definitely somebody will get an opportunity to become a hero for team India," said the captain.
"Maybe in 10 to 12 years' time you will have a cricketer who will be very successful. But to say whether they will replace Rahul or talent like that, it's difficult."
While many are line to fill in the gaps in the coming future, Dhoni paid tribute to one of the greatest Test cricket batsman in the history.
"He was someone who loved challenges. He is someone who will definitely be missed. He is the ideal role model and a good ambassador for the sport."
The Asia Cup will be contested by India, Pakistan, Sri Lanka and hosts Bangladesh.
The first game of the biennial event is between Bangladesh and Pakistan on Sunday.
India will play against Sri Lanka on Tuesday, March 13 in Dhaka.


text:
The Takeaway
Promoting a Culture of Science in the United States
There are many stereotypes associated with the sciences, including the ideas that scientific fields are out-of-reach, too intellectual, or exclusively for men and academia.
Today, Susan Hockfield, outgoing president of M.I.T., discusses these problems and says that the United States must create a culture of the sciences in order to generate interest in the masses. She also urges women to pursue careers in engineering and science and discusses the United States' approach to stem cell research.


text:
December 1st, 2014
Episode 402 of 447 episodes
In this week's magazine, Burkhard Bilger writes about the children who compete in rodeo in his home state of Oklahoma. Bull riding is the most dangerous sport in the world, and it's become even riskier in recent years, as bull breeders have begun selecting for extreme aggression. But in the families Bilger interviewed for his story, little boys as young as three or four years old participate in rodeo events, and begin riding bulls around the age of ten. Bilger and Mark Singer, another staff writer and Oklahoma native, join host Amelia Lester on this week's Out Loud podcast to discuss the kids who compete in rodeo, the parents who let them do it, and the attraction of trying to ride an angry two-thousand-pound animal. As Bilger describes it, when the kids start out, riding sheep and calves, "it's like the best bumper-car ride you've ever been on. And then what you're doing is just gradually turning up the volume. Or another metaphor might be the frog in the water that's getting turned up hotter and hotter until it dies."
You'll need to sign in to write a comment.
Welcome The Vox Markets Podcast with Justin Waite A new episode is released daily, giving you interviews with CEO's, market commentators and the stock of the day as suggested by listeners to the podcast.


text:
Please select a Finish to check availability, or to add this item to your Shopping Cart or Wish List.
K&N Oil Filters are designed to satisfy the needs of racers and engine builders as well as the average motorcycle or ATV owner who wants the best oil filter available. K&N Oil Filters feature synthetic medias that trap 99% of harmful contaminants without compromising oil flow rates.
The K&N 'wrench off' oil filters feature a 17mm nut affixed onto the end that allows for easy installation and removal. The 17mm nut is cross-drilled making it super easy to throw on a safety wire. Its heavy duty canister and base plate protect against punctures while the double-rolled seal and pre-lube gasket ensure a secure seal.
S1 White Lightning 1998
Harley Davidson
Dyna Super Glide CVO/SE FXDSE 2007
Dyna Super Glide CVO/SE FXDSE2 2008
Dyna Wide Glide CVO/SE FXDWG2 2001
Dyna Wide Glide CVO/SE FXDWG3 2002
Electra Glide CVO/SE FLHTCSE 2004
Electra Glide CVO/SE FLHTCSE2 2005
Electra Glide Ultra Limited CVO/SE FLHTKSE 2014
Road Glide CVO/SE FLTRSE3 2009
Road Glide CVO/SE FLTRSEI 2009
Road Glide CVO/SE FLTRSEI2 2001
Road Glide Ultra CVO/SE FLTRUSE 2011
Road King CVO/SE FLHRSE3 2007
Road King CVO/SE FLHRSE4 2008
Road King CVO/SE FLHRSE5 2013
Road King CVO/SE FLHRSEI 2002
Road King CVO/SE FLHRSEI2 2003
Road King Special FLHRXS 2017
Softail Convertible CVO/SE FLSTSE 2010
Softail Convertible CVO/SE FLSTSE2 2011
Softail Convertible CVO/SE FLSTSE3 2012
Softail Deluxe CVO/SE FLSTNSE 2014
Softail Deuce CVO/SE FXSTDSE 2003
Softail Deuce CVO/SE FXSTDSE2 2004
Softail Fat Boy CVO/SE FLSTFSE 2005
Softail Fat Boy CVO/SE FLSTFSE2 2006
Softail Heritage FLST/I 2006
Softail Springer CVO/SE FXSTSSE 2007
Softail Springer CVO/SE FXSTSSE2 2008
Softail Springer CVO/SE FXSTSSE3 2009
Street Glide CVO/SE FLHXSE 2010
Street Glide CVO/SE FLHXSE2 2011
Street Glide CVO/SE FLHXSE3 2012
Ultra Classic Electra Glide CVO/SE FLHTCUSE 2006
Ultra Classic Electra Glide CVO/SE FLHTCUSE2 2007
Ultra Classic Electra Glide CVO/SE FLHTCUSE3 2008
Ultra Classic Electra Glide CVO/SE FLHTCUSE4 2009
Ultra Classic Electra Glide CVO/SE FLHTCUSE5 2010
Ultra Classic Electra Glide CVO/SE FLHTCUSE6 2011
Ultra Classic Electra Glide CVO/SE FLHTCUSE7 2012
Ultra Classic Electra Glide CVO/SE FLHTCUSE8 2013
|Product Style
|RevZilla Item #
|Manufacturer Product #
|In Stock: Ships within 24 hours
|In Stock: Ships within 24 hours
Orders $39.99 and over qualify for our fast free shipping offer. Click Here for FREE Shipping Terms and Conditions.
Please see our International Orders page for orders shipping outside the US.
We show real-time availability on this product page when you select the size/color item you want. Most items will ship the same business day an order is placed,however if an item requires additional processing time a message will be shown indicating such.
See our full Shipping Policy for further details.
If your order arrives and it is not right, we will we'll fix it, NO NONSENSE, we promise.
Doesn't fit or just not happy with it? You can return any new, unused and unaltered item within 30 business days of delivery receipt of your item. We will issue a full refund to your original payment method.
See our full Return Policy for all of the pertinent details.
Have a functionality, fit, or otherwise product-specific question about the K&N Oil Filter KN-171? Ask it here! If you have a customer service or shipping question, please direct it to our Customer Service department.


text:
Write Your Own Review
Men's Ahnu Tamarack BootDon't be afraid of venturing out into the cold when you're wearing the Tamarack. Equipped with a cozy faux shearling lining and a breathable, waterproof membrane, this winter hiker/après ski boot is surprisingly warm without all of the bulk. The rugged Tamarack pulls on easily and provides comfort, stability and traction with its integrated Numentum™ technology and slip-resistant lugs, so your footing is secure in even the most challenging cold-weather conditions.6269


text:
Surfin' Safari-The Beach Boys-1962 MP3
Original song, oldie.
The Beach Boys ~ Surfin' Safari (HQ) MP3
This fantastic song by "The Beach Boys" was released in 1962 and only made it to number 14 on the billboard hot 100, hmm how crazy was that.. The song was ...
The Beach Boys - Surfin' Safari (1962) MP3
The Beach Boys interpretan Surfin' Safari en una de sus primeras grabaciones conocidas.
The Beach Boys - Surfin' Safari (Lyrics on screen) MP3
Song - Surfin' Safari Artist - The Beach Boys Album - Surfin' Safari.
Beach Boys - Surfin` Safari MP3
Buy your copy here on iTunes: https://itunes.apple.com/album/surfin-safari/id703606889 BEACH BOYS - SURFIN SAFARI - LYRICS Let's go surfin' now ...
The Beach Boys-Surfin' Safari Video Clip (HD HQ 1962) MP3
Műfaj:Rock Stílus: Surf, Pop Rock Év: 1962.
Surfin' Safari (Mickey's Fun Songs) MP3
Taken from Beach Party at Walt Disney World. Originally performed by The Beach Boys. Disclaimer: Disney made this sing-along, not me. I own nothing.
Beach Boys - Surfin'Safari (One Man's Challenge 1962) MP3
Beach Boys - Surfin'Safari, One Man's Challenge tv appearance 1962. Probably the first tv appearance of the band. With David Mark instead of Al Jardine.
Surfin' Safari - The Beach Boys (with lyrics) MP3
Surfin' Safari by The Beach Boys with lyrics on screen. Written by Brian Wilson and Mike Love.
Surfin` Safari - The Beach Boys (HQ) MP3
Surfing footage , The Beach Boys 1962 , I own no rights .
Jan & Dean - Surfin' Safari MP3
Jan & Dean - Surfin' Safari.
The Beach Boys - Surfin' Safari Live MP3
Live at Royal Albert Hall, London 30/05/2015 - WATCH THE VIDEO IN HD! Check out our GIG REVIEWS & VIDEOS on www.the-gig-channel.com Follow us on ...
Mission Beach Surfin' Safari MP3
Perfectly fun day surfing Mission Beach! Mom requested Beach Boys, so here it is :) Filmed with GoPro Hero 3 and Ho Stevie! mouth mount: ...
Ramones - Surfin' Safari MP3
track from the Acid Eaters Japanese Version (The Beach Boys cover)
Oszibarack - Surfin' Safari MP3
Written by Agim Dzeljilji & Patrisia Hefczynska Song performed by Oszibarack. Included in " Plim Plum Plam" album. P&C 2008 2.47 Production Published by ...
- R5 TV - Surfin Safari. ITA ; MP3
Scusate se non ho tradotto ogni singola parola di quello che dicevano, ma era davvero difficile visto quanto parlano veloce. Il senso però è quello. Ho saltato ...
Surfin Safari - Alvin and the Chipmunks MP3
Alvin y las ardillas.
Beach Boys - Surfin Safari Guitar Lesson Preview MP3
http://www.TotallyGuitars.com Learn to play Beach Boys - Surfin Safari Guitar lesson - sample guitar lesson. Surfin' Safari- Brian Wilson and Mike Love wrote ...
SURFIN SAFARI for the UKULELE - UKULELE LESSON / TUTORIAL by "UKULELE MIKE" MP3
SURFIN SAFARI for the UKULELE - UKULELE LESSON / TUTORIAL by "UKULELE MIKE"
Melopea Melopienso (Surfin Safari) MP3
31 de mayo de 2.013, Melopienso tributando a Melopea con uno de los temas importantes de la banda cántabra "Surfin Safari". www.insonoro.com.
The Beach Boys - Surfin' Safari (Guitar Cover) MP3
The title track of The Beach Boys 1962 Capitol Records studio debut album. Surfin' Safari was written by Brian Wilson and Mike Love. Hite Morgan, Nick Venet, ...
Beach Boys - Surfin' safari MP3
un clip des beach boys par des Iéseg!
The Beach Boys-Surfin' Safari~Surf City~Surfin' U.S.A.(Live In Japan 1991) MP3
Live at Budokan in Japan November 2, 1991.
How to Play Surfin' Safari MP3
How to play Surfin' Safari by The Beach Boys on guitar.
The Beach Boys - Surfin' Safari MP3
The Beach Boys are an American rock band, formed in 1961 in Hawthorne, California. The group was initially composed of brothers Brian, Dennis and Carl ...
R5 TV - Surfin Safari! MP3
Anarky in the UKelele / The Beach Boys - Surfin Safari MP3
Pabli Ramone en Ukelele Concert Pepu Vicious en Ukelele Tenor Luc Strummer en Ukelele Soprano.
Surfin Safari - The Beach Boys (Bass Cover) MP3
I do not own the rights to this song. It is merely a cover song NOT for monetary gain. If you need a simple bass rift to work out your arms, this is the song. Whew!


text:
Despite cautious optimism from teammates and superiors, it seems the soonest weŌĆÖll see Jason Witten on the field is Week 2.
According to ESPNŌĆÖs Ed Werder, the Cowboys tight end, who suffered a lacerated spleen this preseason, is ŌĆ£extremely unlikelyŌĆØ to play in WednesdayŌĆÖs regular season opener against the Giants. The team considers the injury to be of the 4-6 week variety.
Witten reportedly will undergo another scan on Tuesday to get a clearer indication of his progress.
Dallas will have eleven days to prepare for a late-game tilt in Seattle on September 16. The extra time obviously should work to the 30-year-oldŌĆÖs advantage, assuming heŌĆÖs on pace in his recovery.
Owner Jerry Jones recently expressed optimism regarding WittenŌĆÖs availability, saying itŌĆÖs ŌĆ£not impossibleŌĆØ to think he could suit up against the division rival Giants. Ditto quarterback Tony Romo, who believes heŌĆÖll ŌĆ£probablyŌĆØ have his favorite target in tow.
It doesnŌĆÖt appear to be, however.
(Photo: Jeff Gross/Getty Images)
NFL News
Delivered to your inbox
You'll also receive Yardbarker's daily Top 10, featuring the best sports stories from around the web. Customize your newsletter to get articles on your favorite sports and teams. And the best part? It's free!
|Best of Yardbarker
|NFL News
|MLB News
|NBA News
|NHL News
Today's Best Stuff
For Publishers
Company Info
Follow Yardbarker
|© 2017 Yardbarker, Inc. All rights reserved. Use of this website (including any and all parts and components) constitutes your acceptance of these Terms of Use and Privacy Policy. (What's New?) Ad Choices


text:
Crazy for sewing tote bag 6x10 in the hoop machine embroidery design
Sweet Pea Machine Embroidery Designs
This beautiful sewing tote bag is an in the hoop machine embroidery design.
The design comes in one size for the 6x10 hoop. Requires eight hoopings and some sewing on your sewing machine.. The bag is completely lined and finished beautifully.
Finished size:
6x10 - 48cm wide x 31cm high (19"x 12")
There is a panel with a zipper pocket included and you can use any combination of panels you like. There's even somewhere to clip some useful tools on.
Full photo instruction and directions are included with your purchase.
It is our intention to offer you the best service always and for our designs to be the highest quality in the market.
Designs come in all formats except ART. Bernina's can use EXP. Formats pes, hus, sew, xxx, jef, vp3, vip, mit, dat, dst, exp etc. All formats are included with your purchase.
Instructions are included in english and german.
If you wish to add a zipper closure to the tote bag please see our blog article
or if you want to add pockets to the inside see our blog article


text:
Results could not be displayed.
Browse our collection of gorgeous Christmas tree toppers to complete the holiday look. Choose from a vast selection, which features traditional, unlit angels and stars and vibrant, illuminated novelty toppers. Whether you have a real or artificial tree, we have tree toppers for every style.


text:
Discussion in 'Styling and Customization Questions' started by Wesker, Mar 17, 2014.
Is there a mod or configuration to allow something like this?
That's already able to be done isn't it? Just a little extra styling to remove the last post from it and it should work.
I'm not sure. Do you know exactly where to make the style changes?
Style Properties -> Forum / Node List
Okay now I see why they disabled it. The CSS is off. Where do I make the necessary CSS changes?
The default style works out of the box so you will have to inspect the code for your custom style.
Do you know specifically where I would look to make the changes with the custom template? Where do you make the changes in the default style?
EXTRA.css, but since you are using Flexile (if I remember correctly) it may have to be done somewhere in that style.
As far as I am aware, Flexile hasn't been updated for 1.3
Regardless, you will need to ask for support in the dedicated discussion thread.
Alright Ill play around with it later and see what I can do. Will update Flexile Thread if I'm able to fix it for others with similar issues. Thanks guys!
Didn't have time to work on this until now. The flexile theme is using the "Node List" font size inside Style Properties: Forum / Node List. While I understand each theme is different, is there a specific category that handles the text size and css for "Show sub-forums popup"
I've been able to fix the issue but it also updates all the node titles and just trying to edit the size of the sub forum nodes that appear under the primary node.
You will need to ask in the Flexile thread for support with that.
Separate names with a comma.