text:
Dear customer, kindly fill in your name, contact number and email, so we can inform you when the new stock of this product arrive.
Email your friend about this product
The Going Merry, famed worldwide as the first ship of Monkey D. Luffy's Straw Hat Pirates, is now immortalized by Bandai as a fantastic plastic kit! Bandai includes nylone rope for the anchor, a display stand, a big cannon on the deck, cargo such as barrels and crates, and more! This nonscale kit measures 28cm (just over 11 inches) long upon completion. The kit is molded in a variety of colors, including dark brown, tan, white, cream, and gold.
This version depicts the Going Merry in flight mode! (NOTE: The model doesn't really fly!) Includes 8 unpainted figures.
- Accessories: Figures × 8 (Luffy, Zoro, Nami, Usopp, Sanji, Chopper, Robin,
South Bird), Tal, crates, rope ladders, guns, orange trees × 3, display base
We currently charge shipping fees on 'Per Order' basis. Depending on your location and shipping mode the price will vary, please refer to chart below for details.
All our orders are packed and shipped within 24 hours upon payment confirmation. We use POSLAJU / Air Parcel / TA-Q-BIN / ABX for most of our orders . They are able to ship overnight to most cities and towns in the Peninsular Malaysia, however you should allow 1-3 business days in case any unexpected delays. East Malaysia orders may often take 4 to 5 extra days, so please allow 5 to 7 business days for delivery.
We will provide the POSLAJU / Air Parcel / TA-Q-BIN / ABX tracking number by email once we sent out the goods so that you can check and track the shipment status online.
Note: Please white-list our email address email@example.com in your email account, so when we email the shipping status to you, it won't go into the Junk mail folder.
PACKAGING: We use hard cover boxes for all our delivery to avoid damage on the item, kindly refer to the below picture for our packaging
1.) What is a tracking code /shipping code?
1.) How do I build the gundam?
2.) How many grade of gundam are there?


text:
45640, OH Rent to Own Homes
|3 Listings Found
Transfer with No Active Leases (ATO) Hamilton Township, Jackson County Oak Hill Schools Part of Parcel
$59,900 - Rent to Own
Jackson, OH 45640Details »
Absolutely stunning 159 acre property now available in Jackson County. Words cannot describe this immaculate country estate with it's...
$639,900 - Rent to Own
Jackson, OH 45640Details »
This 0 sq. feet Property has 0 Beds(s) and 0 bathrooms(s). The Loan estimate Balance/Default amount is $43,000.00. This is an Property...
$43,000 - Foreclosure
Jackson, OH 45640Details »
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subset: commoncrawl


text:
Green Peppers
© All Rights Reserved
These green pickled peppers called my attention while we were visiting the medieval market of Suria. I took many pictures there so you will hear the name of this town near Barcelona in many of my posts. I also like the color of those olives back there. I am not fond of peppers myself but they really make a good subject for a picture. I have managed to post today through one of my friends. Have a nice weekend!


text:
Robust earnings help Europe stocks resume rally
* FTSEurofirst 300 up 0.8 pct, Euro STOXX 50 up 0.9 pct
* Sanofi share drop represents $10 bln market value wipeout
* So far, two-third of companies meet/beat forecasts
By Blaise Robinson
PARIS, Oct 28 (Reuters) - European shares rose in early trade on Tuesday, reversing the previous session's dip as better-than-expected results from a number of blue-chips including pharma group Novartis and bank UBS helped lift sentiment.
Bucking the trend, shares in pharma group Sanofi sank 7.1 percent after posting weaker-than-expected quarterly sales, blaming pricing pressures on its lucrative diabetes business.
"This is disappointing for one of the company's most important growth engines," Berenberg analysts wrote in a note.
The sharp drop in the stock - its biggest one-day slide in five years - represented a wipeout of 7.8 billion euros ($9.9 billion) in market value of France's biggest listed company and one of Europe's largest blue-chips.
At 0915 GMT, the FTSEurofirst 300 index of top European shares was up 0.8 percent at 1,315.00 points, reversing a 0.6 percent fall on Monday. Continuación...


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text: Introducing the Hunan Winged Bean Pods, seeds, blossoms and tubers — you can eat every part of the hunan winged bean. Winged Bean Pods are long, flat and covered with frilly "wings" along four edges. ROB CARDILLO The tender shoots and white or pale blue flowers can be eaten raw, and add a lot of pizzazz to summer salads. WILLIAM D. ADAMS Winged beans are commercially harvested when they reach 4 to 6 inches in length, but many gardeners prefer them at 2 to 3 inches while they're still tender. BAKER CREEK HEIRLOOM SEED COMPANY When the pods turn black and dry out, you'll have seeds ready to save for next year's crop. HELEN TAK Winged beans are prized for the many ways you can enjoy not only the seeds and pods, but also the shoots, blossoms and tubers. MA. MYLA B. CREENCIA The vines, which can reach up to 10 feet in length, produce lots of heavy pods; be sure to support them with a sturdy trellis. FLORIDATA Read Article


text:
- Description
American Eagle CT-3070 Coffee TableIncludes
1 Coffee TableDimensions
Coffee Table, 51"L x 27.5"W x 14"H
More from this collection: EK-L403
- Additional Information
Additional Information
SKU 15710372 MPN CT-3070 GTIN No Brand American Eagle Collection EK-L403 Assemble Length 51 Assemble Width 27.5 Assemble Height 14 Assembly Required Yes Material MDF Finish Black Sizes No Styles Modern Shipping Time 2-3 Weeks Free Shipping No
- Reviews
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text: Living in Southern California I can't help in your search. I was trained by our local sheriff's department,and on my own attended Front Sight for a 4 day handgun course a couple of times. I suggest you get together with a friend and take a course at Front Sight. They have good 2 day classes, and this time of year the weather is bearable. I have seen them train people that have NEVER handled a firearm, and leave the class with tons of confidence. Sorry I couldn't be of more help.Good luck in your search. Oh,and Front Sight is not far from Las Vegas.


text:
Ketchup bottle
There are two worlds of bottom snacking, the world of BDSM and thw world of ketchup bottles. The company, Dressing for Pleasure, a specialist in fetishwear and all things S&M releated, married these two worlds by putting stickers of enticing rear ends on the bottoms of ketchup bottles. Thus inviting bar patrons to explore a completely different appetite while addressing their hunger.
Direct advertisment created by JWT, United States for Dressing for Pleasure, within the category: Recreation, Leisure.
Chief Creative Officer: Ty Montague
Executive Creative Director: Kash Sree
Creative Director: Raphael Milczarek
Copywriter: Adam Noel
Art Director: Jackie Anzaldi
Photographer: Ali Weheda
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text:
- #36881 -- $699,000 -- 3.17 Acres
- 5 Bedrooms - 3 Full Bath - 1 Half Bath - 3353 Square Feet
- Location: Bearsville NY
- 5 Bedrooms and 3 1/2 Baths
- Huge mid-century Country Kitchen
- 2 Master Bedroom En-Suites
- Large & Private Screened Porch
- Oversized Entertainment Deck
- 125' on Vredenburgh Creek
- Walk to the Bearsville Complex
- Call or Text Joan Roberts 845-853-9095
- Call or Text: (845) 853-9095 for more INFO
- OR.... EMAIL: firstname.lastname@example.com for more INFO
Woodstock Contemporary Cottage
Celebrity Home in Woodstock
#36881 -- $699,000
45 West Ohayo Mtn Rd, Bearsville NY
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LEE MARVIN AND HIS WIFE, PAMELA were childhood sweethearts who found their way back to each other, and loved to be in their hometown of Woodstock or their adopted town of Tucson, AZ when they could escape from the Hollywood lifestyle. THEIR FAVORITE PROPERTY WAS THIS EXPANDED CAPE IN WOODSTOCK. Because they had spent so many years renting and loving this home, Pamela bought it years ago. It is now on the market. It has been lovingly re-created with all of the character and joie de vivre that was so important to them both. The 5 BEDROOM, 3 1/2 BATHROOM HOME on one of Bearsville's most sought after, no outlet roads. EVERY ROOM HERE TELLS THE STORY OF A BOND SO STRONG,IT COULD NOT BE BROKEN!
The 3.17 Acre property has about 2.63 around the house and a smaller .54 acres directly across West Ohayo Mtn Rd. That property across the road goes all the way to the Vredenburgh Creek, where you can have your own private shangrila. The house has 5 bedrooms with two Master Bedroom en-suites - one on the first floor with huge closets, a large Master Bath and a lovely sitting room. The other Master is located on the second floor with large closets, built in chests, an attached dressing room or office and a full bathroom. There are three more bedrooms on the second floor along with another full bath. The main floor features also include a warm and cozy Den with a fireplace overlooking the very large deck as well as a large formal Living Room and Formal Dining Room. And then there are the country Kitchen and Breakfast Rooms. Large, well lit featuring a Double Oven, a large Jenn Aire Cook-top with a huge and decorative exhaust fan. Also a dishwasher, a compactor and many cabinets and storage areas. This is a GREAT party house! The main floor also features another half bath, several pantry areas, an amazing and huge screened in porch along with a very large storage room, 2 car garage and a full unfinished walk-out basement. The set up could lend itself well for a full time year round home, a thriving B&B, a second home or weekend home. So many possibilities... A gentle walk to the Bear Cafe complex, the Sawkill Creek and the Bearsville post office and market. A short walk to catch the Trailways Bus on 212. Perfect location!
45 West Ohayo Mtn Rd, Bearsville NY, 12409
Joan Roberts
email: : firstname.lastname@example.com
call or text (845) 853-9095


text:
Advanced Beginner Salsa Workshop - Copa Theme
Salsa Dance Practicar
Monday, July 14, 2014 from 7:00 PM to 8:30 PM (PDT)
Who's Going
San Francisco, California
London, United Kingdom
Florence, Italy
Event Details
When & Where
La Belle Fit Studio & Spa
219 Haddonfield Berlin Road
Cherry Hill,
Monday, July 14, 2014 from 7:00 PM to 8:30 PM (PDT)
Add to my calendar
Salsa Dance Practicar
I am Luther Lockard and I own/operate Salsa Dance Practicar, a meetup group for anyone in the Delaware Valley who is interested in supplementing their existing formal dance training and lessons by practicing and training with others seeking to improve their Salsa and Latin dance skills. The purpose and rational for the group is to provide a forum and means to bring together people to practice and rehearse movements and routines learned in formal training and lessons which are not appropriate to try in social dance settings. This also provides a "bridge" for learning between and outside of formal lessons and provides an opportunity for more flexibility for experimentation outside of the social dance setting. The format of the events will "Not" include formal lessons and instructions as the purpose is to bring together people to share and practice together in their own pace and comfort. However, we have a YouTube Channel that we will use as a reference and resource for practice: http://www.youtube.com/channel/UCnCntbzkg8_....
In addition to the practice sessions, , we will have special workshops and promotions on a regular basis at various venues.
Follow us on our YouTube Channel for the latest updates on instucttional tips:
As a value added bonus, seminars and workshops will be held on Posture, Balance, and Flexibility, all of which are necssary to condition and prepare the body for efficient dancing.
When possible, a Posture, Blance, or flexibility tip and/or exercise wil be included in the practice sessions.


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text:
Karissa Anne Model
Colliers EyePhotographer Male Barre, Vermont, US
My Website: Collier PhotosMy MM URL: http://www.modelmayhem.com/dmcolliers
Mayhem # 1326330
About MeMy Name is Mark. I am a freelance photographer based in Vermont. While I am pretty versatile and can shoot anything, I tend to specialize in sports, illustrations and editorial images. I am more than willing to work with new models and trade my time for shots/cd. The more fun the shoot, the cooler the idea, the more likely I am to be interested. My work leans pretty heavily in the editorial direction, but fine art is my passion, and I just don't get to do enough. I have a studio and equipment (including lighting) to shoot on location.
My work has been published all over the world and in: The Times Argus, The Burlington Free Press, The Washington Post, The Bangor Daily News, The Dallas Morning News, The L.A. Times, The The Syracuse Post-Standard, The Albany Times Union, Labor Notes, CNN, CNN.com, The Rutland Hearld, India Today, The New York Times, The New York Times, Magazine, WCAX, The Washington World, Stone in America, The Hartford Courant, The New Haven Register, The Saint John's Telegraph-Journal, The Boston Globe, Ski Racing Magazine and many, many others.
Have you worked with Colliers Eye? Add Credits for Colliers Eye! >>
Karissa Anne Model
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text:
Alfa's big bet on the Stelvio
Alfa Romeo is betting on the Stelvio and its fun-to-drive characteristics to boost the appeal of what is expected to become the brand's best-seller. The Stelvio is the first SUV from the Fiat Chrysler brand.
Read More »
■ Alfa Romeo Stelvio
Opel moves Insignia upscale
Opel hopes a wide selection of advanced safety equipment in its new Insignia will move the model upmarket and lure buyers from premium brands. ...
Softer Land Rover Discovery keeps its go-anywhere skills
Land Rover is determined to demonstrate that even though the fifth generation of the Discovery has been made softer and more luxurious than its predecessor, it remains tr ...


text:
22 photos
$3,490,000 11.2 acres 6 Beds | 8 Baths | 3 ½ Baths | 10287 sqft 575 South St, Pittsfield, MA 01201 - Berkshire County
11.2 acres Pittsfield, MA
The Morewood is a masterpiece of historical restoration, every room draws you in to a place where guests are treated like family. It represents the design influence of the early work of Sir Edwin Lutyens, with architecture adapted from the Elizabethan period, and nestled into its incredible English gardens. A 10,000 sq.ft. Tutor style brick main house with 6 bedrooms, 8 bathrooms.


text:
I first visited James Bach's blog over two years ago (New Team System Stuff – 2004-08-28), but I must've misplaced his RSS feed during a reader change, or system refresh. Anyway, a post on the Tyner Blain blog (Rinse, Lather, Repeat – 10 Reasons to Repeat Tests) helped me re-discover James' excellent blog.
I'm currently seeking additional blogs that focus on testing as a discipline. I already have The Braidy Tester and Sara Ford's WebLog. If you'd like to recommend some, please comment this post. Thanks.


text:
If an item you ordered from TextbookRush does not meet your expectations due to an error on our part, simply fill out a return request and then return it by mail within 30 days of ordering it for a full refund of item cost.
Learn more about our returns policy
Description: After the Cold War, Africa earned the dubious distinction of being the world's most bloody continent. But how can we explain this proliferation of armed conflicts? What caused them and what were their main characteristics? And what did the world's governments do to stop them? In addressing these and other questions, Paul Williams offers the first comparative assessment of more than two hundred armed conflicts which took place in Africa between 1990 and 2009 - from the continental catastrophe in the Democratic Republic of Congo to the environmental disaster in the Niger Delta and mass atrocities in the Sudan. Taking a broad comparative approach to examine the political contexts in which these wars occurred, he explores the key ingredients that provoked them and the major international responses undertaken to deliver lasting peace. Part I, Contexts provides an overview of the most important attempts to measure the number and scale of Africa's armed conflicts and provides a conceptual and political sketch of the terrain of struggle upon which these wars were waged. Part II, Ingredients analyses the role of five widely debated features of Africa's wars: the dynamics of neopatrimonial systems of governance; the construction and manipulation of ethnic identities; questions of sovereignty and self-determination; as well as the impact of natural resources and religion. Part III, Responses, discusses four major international reactions to Africa's wars: attempts to build a new institutional architecture to help promote peace and security on the continent; this architecture's two main policy instruments, peacemaking initiatives and peacekeeping operations; and efforts to develop the continent. War and Conflict in Africa will be essential reading for all students of international peace and security studies as well as Africa's international relations.
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List price: $17.99Copyright year: 2011Publisher: Polity PressPublication date: 9/2/2011Binding: PaperbackPages: 320Size: 6.75" wide x 9.75" long x 1.00" tall Weight: 1.276Language: English
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I lived in Battersea from about 1939 to 1949 when we moved to Hornsey in North London. We lived with my grandparents who owned the Southern Dining Rooms, a transport café opposite the marshalling yards, just at the end of the Dogs Home bridge. My grandmother's cooking was renowned up and down the country by the lorry drivers who regularly came to the café. It is no longer there. We used to go to the nearby fish shop and buy a penny worth of crackling. I have many happy memories of playing in Battersea Park, my mother would see us across the very busy road and send us off with jam sandwiches and lemonade for the day. We always made a stop by the telephone box on the way to press Button B and were often rewarded with (I think) tuppence when someone had forgotten to retrieve their money. Being close to the marshalling yards and Battersea Power Station we were very vulnerable and I remember during the war we all had to go down to our cellar whilst an air raid was on, except my grandfather who refused to go down. Does anyone remember 'Blind Bill'? He used to stand opposite our side entrance in Savona Street and was loved by all the children. We always knew when there was going to be an air raid as Bill could sense the vibrations and would suddenly be off. I had a friend called Jean Lomax who lived in the flats in Savona Street and we used to have great fun playing on the bomb sites, picking the flowers that were still growing in the gardens of the bombed houses.
A memory shared byon Jun 7th, 2014.
Tue Aug 12th 2014, at 12:49 pm
Sat Mar 21st 2015, at 8:12 am
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text:
6:00 P.M. 1133 66Th ST COUNCIL CHAMBERS
(Full detail and supporting documents for each item can be found at www.windsorheights.org If you do not have web / internet access, and would like the supporting documents and information, please call City Hall by 3:00 p.m. the day of the meeting and we will provide you a hard copy at the meeting)
Notice to the Public: In consideration of all, if you have a cell phone, please turn it off or put it on silent ring. The use of obscene and vulgar language, hate speech, racial slurs, slanderous comments, and any other disruptive behavior during the Council meeting will not be tolerated and the offender may be barred by the presiding officer from further comment before the Council during the meeting and/or removed from the meeting.
1.Call to Order/Pledge of Allegiance
2.Roll Call
3.Approval of the Agenda Council Packet Memo
4.Comprehensive Plan Review with Planning and Zoning Commission
5.Approval of the Consent Agenda: Any item on the Consent Agenda may be removed for separate consideration.
A. Approval Minutes of the Regular Council Meeting held on November 7, 2016
B. Approve Payment of Cash Disbursements
C. Approve October's Treasurer Report
D. Approve Urban Renewal Report for FY 15/16
E.Cancellation on Monday, December 19, 2016 Regular Council Meeting
F. Approve Resolution No. 16-1172 Stipulation of Bargaining Unit as Amended
6.Public Comment: This is time set aside for comments from the public on topics of City business other than those listed on the agenda. No action may be taken. Please come to the podium, state your name and address for the record and keep your comments to no more than 5 minutes.Registered Comment –
7.Old Business:
A. Discussion and Appropriate Follow-up on Windsor Heights Living Contract Renewal
8.New Business:
A.Discussion and Appropriate Follow up on Economic Development Program Application from William Turf Farms, Inc. for 6611 University Avenue
1. Resolution No.16-1169, A Resolution Authorizing Internal Advance for Funding of Urban Renewal Project
B.Resolution No.16-1170, A Resolution Approving the Walnut Creek Watershed Master Plan
C. Discussion and Appropriate Follow-up on MSA Contract Renewal
D. Resolution No. 16-1171, A Resolution Approving Tax Increment Financing Indebtedness Certification and Directing the Certification to be Filed No Later than December 1, 2016
9.Reports: City Administrator/Mayor/Council/Staff/Board, Committee, Commission updates Communciation coordinator/clerk Police Department Report
10.Adjourn to 6:00 pm on Monday, December 5, 2016, for a council meeting at Council Chambers


text:
RIKEN, Nanophotonics Laboratory, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
Osaka University, Immunology Frontier Research Center, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan
Osaka University, Department of Applied Physics, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
We report the first demonstration of deep ultraviolet (DUV) Raman imaging of a cell. Nucleotide distributions in a HeLa cell were observed without any labeling at 257 nm excitation with resonant bands attributable to guanine and adenine. Obtained images represent DNA localization at nucleoli in the nucleus and RNA distribution in the cytoplasm. The presented technique extends the potential of Raman microscopy as a tool to selectively probe nucleic acids in a cell with high sensitivity due to resonance.
Raman scattering spectroscopy is a useful tool for biochemical and analytical study of a molecule of interest. The technique probes a specimen without any molecular labeling. This is because Raman scattering light has an energy shift, which is inherent to the chemical structure of a molecule, relative to the excitation light. Analyzing a spectrum derived from a molecule, we can collect the valuable information on chemical and physical characteristics of the molecule, such as molecular structure and electronic state.
When combined with microscopy, Raman spectroscopy (Raman microscopy) becomes a label-free imaging technique to reveal molecular distributions in a cell.1–5 Raman microscopy applications were reported for sugar,6 lipid,7–9 protein,8–12 metal complex,8,9,11 nucleic acid,5,12,13 and carotenoid13 molecules in cells. With the help of fast imaging techniques, such as slit confocal8,9 and nonlinearscattering14,15 microscopy, Raman spectroscopy achieved dynamic molecular imaging of cells.
Varying the excitation wavelength, Raman microscopy probes different molecular species in a cell. Blue/green excitation light highlights visible pigments such as carotene13 and heme-complex8,9,11 in a cell. This is caused by the resonance Raman effect, where the scattering cross-section increases when the excitation wavelength is close to a molecular electronic transition band. Optimal resonance results in enhancement of up to 106 compared with the nonresonant case. Even without significant resonance, at red to near-infrared wavelengths, abundant molecules in a cell are highlighted, such as lipid7 and protein.5,10,12
Away from the visible spectrum, deep ultraviolet (DUV) resonantly excites Raman scattering of nucleotide bases16,17 and amino acid residues18,19 that have strong absorption bands generated by electronic transition in DUV. Because of resonance, DUV Raman spectroscopy can sensitively probe specific structures, which are not probed by off-resonance wavelengths, and can uniquely measure microstructure and conformation of proteins19 and nucleic acids.20 One study using DUV Raman spectroscopy analyzed the secondary structure of telomeric DNA to study the lifetime of living organisms.21 Another study showed secondary structure changes in myoglobin linked with the Tyr and Trp residues micro-environment.22 The method is also used for cellular level analysis: using resonance, DUV can selectively observe such aromatic compounds in a cell.23–25 Cellular conditions,26 types,27 and metabolic states28 have all been studied using DUV Raman, and these aromatic compounds play important roles in dynamic activities and functions of cells.
In this study, we extended DUV Raman spectroscopy of cells to microscopy imaging, which has not been reported previously. To achieve this, we developed and optimized a conventional stage-scanning DUV laser Raman microscope for cellular imaging with sub-micron resolution while minimizing molecular photodegradation induced by DUV in order to gain sufficient signal before molecules are significantly degraded.
Figure 1(a) shows an obtained Raman spectrum of a HeLa cell together with a single spectrum derived from the substrate. Three prominent bands seen at 1340, 1490, 1580 cm−1 are all assigned to guanine and adenine,29,30 while a band at 1615 cm−1 is attributable to tyrosine and tryptophan.27 We chose the band at 1490 cm−1 for cellular nucleotide imaging. The distribution of the intensity at 1490 cm−1 is shown in Fig. 1(b). In the image, we see two intense circular spots situated close together near the center, indicated by arrows. Comparison with the transmission image shown in Fig. 1(c) confirms that these spots exist inside the nucleus, indicating they correspond to nucleoli containing dense DNA. The comparison also reveals that nucleotides are distributed in the cytoplasm with a high concentration. Such a distribution would be expected due to RNA existing in the cytoplasm.
DUV Raman microscopy of a HeLa cell: (a) A single spectrum of the cell (solidline) and substrate (dashed line)l (b) a image reconstructed with the intensity at 1490 cm−1; including color scale bar; and (c) transmission image corresponding to (b). The spectrum was numerically processed for the noise removal before used for the image reconstruction; briefly, acquired spectra were first processed with singular value decomposition (SVD), then hyperspectral data was reconstructed with loading vectors that contribute to the image contrast. Finally, each spectrum was processed with baseline background subtraction using polynomial function fitting.8
In some cells, we found a clear difference in the intensity distribution of different two bands (1490 and 1340 cm−1). Figure 2(a) shows two spectra obtained in different locations in a HeLa cell, and the distribution of 1490 and 1340 cm−1 is shown in Fig. 2(b) and 2(c), respectively. In the 1340 cm−1 image, the nucleus is clearly highlighted. The highlighted distribution in the 1340 cm−1 image is understood to represent the DNA distribution in the nucleus.26 On the other hand, the 1490 cm−1 image highlights parts of the cytoplasm outside the nucleus. As mentioned above, the images show distribution of both DNA and RNA in a cell, and the difference between the two distributions is clearly seen in their overlapping image [see Fig. 2(d)]. Then it is suggested that the ratio image of the 1490 cm−1 image to the 1340 cm−1 image (1490/1340) can generate a map of RNA distribution, shown in Fig. 2(e). Note that because this is a ratio image, the denominator was processed by thresholding at an appropriate noise floor before generating the ratio image. The image is not very clear, but does show a characteristic distribution of RNA in a cell, which is relatively low in the nucleus and higher in the cytoplasm.31 The results show how DUV Raman microscopy can be used for distinguishing distributions, such as DNA and RNA in a cell without any molecular labeling.
(a) DUV Raman spectrum deriving from different locations in a HeLa cell: (b) and (c) Images reconstructed by intensity at (b) 1490, and (c) 1340 cm−1; (d) ahyperspectral image of two bands. The distribution shown in (b) and (c) are shown in red, and blue, respectively. (e) A ratio image (1490/1340).
The difference in reconstructed images from 1340 and 1490 cm−1 bands may emerge from the difference in the contribution ratio of adenine and guanine to the respective bands. A report experimentally obtaining scattering cross-sections of deoxyadenosine and deoxyguanosine29 supports our idea that scattering cross-sections of adenine and guanine for the 1490 cm−1 band are close, while the band at 1340 cm−1 is dominated by adenine with a slight guanine contribution. This means that the 1340 cm−1 image highlights adenine distribution, while the 1490 cm−1 image shows adenine and guanine net distribution. To our knowledge, there is no proof that either adenine is richer, or guanine is less, in DNA compared to RNA. Further study is needed to conclude the origin of difference between two images; however, the present results and discussions imply that DUV Raman microscopy is capable of distinguishing the distribution of adenine and guanine in a cell.
As mentioned above, molecular degradation initiated by DUV (photodegradation) was a significant challenge,32,33 which has long limited the use of DUV Raman spectroscopy in microscopy. The photon energy is so high that electrons in an absorbing molecule are excited, and the molecule can irreversibly change through energy relaxation such as ionization. In addition, reactive by products generated can destroy nearby biomolecules. Additionally, performance of optical components, such as the lens and detector for DUV, has been largely limited. Thus the photodegradation constrains the acquisition of spectral signal sufficient for molecular identification from a small volume of sample before molecular damage.33 There are some studies reporting DUV Raman microspectroscopy of cells, but to get sufficient S/N, the area for spectral acquisition was relatively large.30,34–36 One report showed 3 × 9 μm2 pixel-based microspectroscopy of paramecium cells,35 while another study on plant samples used 5 μm2 pixels.36 Sub-micron resolution is essential for molecular imaging of a cell.
There were essentially four main challenges overcome to achieve the present technique. The first is to optimize the intensity and exposure duration of excitation. In a previous study, we showed appropriate excitation conditions for Raman microscopy of cells by correlating intensity and exposure with photodegradation during measurement.33 The second is to improve the detection throughput of a microscope (details below). The third is to find the right scanning step of the sample stage. The step was set at 0.5 μm, which is almost twice as large as the Airy disk diameter at the sample plane, so that photodegradation does not accumulate during imaging. The fourth is sample preparation. The fourth is sample preparation and is related to the step size issue mentioned above. In experiments, by determining the onset of photodegradation,33 we can then operate in a range where obtained spectra are not degraded and reconstructed images show clear molecular distributions. Photodegradation limits the effective resolution of the microscope if the photodegradation region exceeds the focal spot. In our experiments (data not shown), the resolution for imaging desiccated cells was as small as 0.35 μm. On the other hand, for in-liquid cells, even cells chemically fixed with paraformaldehyde, the molecular photodegradation region was wider, and therefore the pixel resolution for in-liquid cells can not be smaller than 1.2 μm. Those fourkeys enabled DUV Raman microscopy of a cell with submicron spatial resolution.
Design of the purpose-made microscope is an important part for this research. While similar to experimental setups for visible wavelength Raman imaging,2,3 our system has several unique features. For excitation, the microscope uses the 257 nm cw line from a frequency-doubled Ar+ laser (Coherent, Inc.), suitable for resonant excitation of nucleotide bases.30 Even with desiccated samples, we used a high NA glycerin-immersion objective lens (Carl Zeiss, Inc. NA=1.35) for excitation and scattering light collection from the sample with the smallest possible excitation focal volume. The excitation intensity is adjusted to 0.1 mW/μm2 by an ND filter. A shutter is used for controlling the duration of sample exposure to avoid photodegradation of the sample. Rayleigh scattering is rejected by an edgefilter, and the frequency-processed light is then dispersed by a holographic grating (Princeton Instruments, Inc. 1800 G/mm). We measured the spectra by a back-illuminated CCD (Princeton Instruments, Inc. SPEC-10 2KBUV). Grating dispersion is calibrated with the laser line (0 cm−1), and Raman bands of boron nitride (1370 cm−1) and acetonitrile (2249 cm−1). For imaging, a piezo-electric stage (PI, Inc.) raster-scanned the sample with a 0.5 μm step. Each spectrum is acquired with 0.75 s sample exposure, and all optics are necessarily made of quartz or CaF2.
Due to signal-to-noise considerations, some noise reduction is required. We decomposed hyperspectral images by unwrapping the three-dimensional (3-D) hyperstack into a two-dimensional (2-D) matrix of wavenumber and pixel dimension. We then performed singular value decomposition (SVD) and reconstructed the spectral based on only the top five loading vectors, which contribute strongly to spectral image contrast. For data shown here, use of the top five loading vectors appeared optimum. In some cases a loading vector containing useful information may fall outside the top five, so that manual inspection of loading vectors is still required to ensure that maximum spectral information is retained. Due to the poor signal-to-noise ratio of raw spectra, in some cases, using background subtraction before SVD and reconstruction produced errors. We therefore first applied SVD and reconstruction before considering background removal. The contribution of autofluorescence from tryptophan or other components will be spatially overlapped with the Raman signal, and SVD did not produce complete separation of these spectral distributions. Therefore we did an additional per-pixel polynomial function fit and subtraction to remove remaining background. The resulting images show quite uniform background (dark regions outside the cell) due to the strength of this processing at removing random noise that does not contribute to the spectral information in the data.
It is useful to further discuss spatial variation of spectra to understand how valid this imaging method is for cellular nucleotide imaging. For the image in Fig. 1, a mean spectrum from a large number of pixels in the cell region (n=2225) and then in the substrate area (n=1155) for pre-processed data is given in Fig. 3. Spectra are shown as mean spectra, together with plus/minus standard deviation curves in Fig. 3(a). This shows the spectral variation reproducibility for the measurement. For both the cell and substrate, the standard deviation is low enough that the separation of cell and background is obvious, indicating good reproducibility of the method, even though by nature, pixels in the cell area must vary throughout the whole cell area. Figure 3(b) shows the same measurements, without pre-processing. Clear signals are still observable, allowing the use of raw data, although the usefulness of the processing technique becomes clear. The standard deviation is, again, small and is a similar level to the noise. This is apparent in Fig. 3(c), which shows spectrum from a single pixel. We find that the presented method should be reliable for resonant observation of aromatic component distributions in cells.
Mean spectra of cell and substrate regions for the data set in Fig. 1: The top panel (a) shows pre-processed data (see main text for details); (b) shows raw data. Mean curves are shown together with curves representing plus/minus standard deviation; (c) a representative single pixel spectrum from both the cell area and the substrate region.
In conclusion, we demonstrated the first DUV Raman microscopy imaging of a cell. Using 257 nm excitation, we selectively obtained nucleotide distribution in a HeLa cell, by utilizing the resonant Raman effect. Obtained cell spectral signals from pixels within the cell are well-separated from spectra due to the substrate and are reproducible, making further spectral analysis possible. The presented technique extends the potential of Raman microscopy as a tool to probe nucleic acids in a cell with high sensitivity. Future improvement of the signal detection throughput will further develop the technique as a useful label-free tool to analyze nucleic acid dynamics during biological processes such as apoptosis and mitosis.
At the end, we note the overall picture of the DUV research field. DUV optics and light sources have been specifically developed and revamped in the past decade. Because of this, DUV studies for microscopy31,37 lithography, sterilization, and other fields are growing. Recently, plasmonics, with the features of electric field enhancement and spatial confinement, is also budding in DUV and preliminary applications for enhanced Raman scattering were reported.38–40 DUV research field has a huge potential for future applications.
This study was supported by RIKEN research funding and by JSPS via Grant-in-aid for scientific research S program. The authors thank Dr. Katsumasa Fujita (Osaka university) for valuable comments and discussions.
Yasuaki Kumamoto ; Atsushi Taguchi ; Nicholas Isaac Smith and Satoshi Kawata"Deep ultraviolet resonant Raman imaging of a cell", J. Biomed. Opt. 17(7), 076001 (Jul 03, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.7.076001
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A very spacious one apartment situated in the new part of Cala de Villajoyosa, only 400 meters to the quaint beach of Cala de Finestrat with all its bars, shops and restaurants serving great food for all tastes and pockets, just a five minutes in a car and you will reach various golf courses, commercial shopping centers and the ever popular Benidorm where the entertainment is guaranteed all year round. The apartment offers spacious double bedroom with fitted wardrobes, adjoining is a large bathroom, open plan fully fitted kitchen with utility room, spacious living-dining room of approximately 25 m² leading on to a fully glazed in terrace, there is pre-installation for air conditioned throughout, furnished and ready for immediate occupation. Within the urbanization there are large garden areas, swimming pool, tennis court, function room, sauna and gymnasium, ideal for a couple or to rent out for holiday lets, perfect condition and ready for immediate occupancy.
We can help you to get a mortgage on favourable terms. A residence permit can be obtained with the purchase of this property. Please ask our team for more information.


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Accepting new patients
Complete General Dentistry
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New Patients Always Welcome
RIGID Sterilization Procedures
Family Vision Care
LAKEVIEW VISION CENTER LLC practices in Lakeview, OR. Optometrists offer services which typically include the examination and treatment of common vision disorders as well as the fitting and prescription of corrective eye glasses.
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Sydney to Bali Cruise
Sail Date: February 17, 2016
Reviewed: 1 year ago
Traveled As: Couple
Room Type: Balcony
Enjoyed the great food, activities, excursions and friendly staff.
Please read the comments below to get my feedback on the detailed aspects of the cruise. I hope by now I have reach the minimum of 150 characters to be able to submit this review without it being rejected.
Category Ratings
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|Cabin / Stateroom
|Embarkation and Disembarkation
|Ship Quality
|Destinations and Excursions
|Mt Tamborine tour needs some upgrades to the wine tasting portion. The winery couldn't accommodate the number of people.
|Onboard Activities
PLEASE put cold water and towels by the golf nets. It gets very hot out there!
Yoga, Zumba & Golf classes were excellent.
Really liked lecturers, especially Ken & Adrian.
If computer classes previewed what they were going to teach, we could see if we already know it.
Could we have some happier movies?
|It would have been nice to have more authentic New Orleans Jazz at the brunch.
|Service and Staff
|Always very friendly and helpful!
|Food and Dining


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Galleries in Zagreb City
Browse 81 attractions, meet 29 travelers, 27 tour guides and discover 76 photos
Travelers are voting Klovicevi Dvori Gallery, Art Pavilion and Modern Gallery as the best of 17 galleries in Zagreb City. There is 17 galleries in Koprivnica a city just 97 km from Zagreb City. The most popular of these is Klovicevi Dvori Gallery in Koprivnica. You can find 17 galleries in Zagreb and 17 galleries in the country of Croatia. Our members favorite in Zagreb is Art Pavilion and Klovicevi Dvori Gallery is the top pick in Croatia. Want more? Check out our map of galleries in Zagreb City.
Klovicevi Dvori GalleryPlaces To Visit GalleriesJezuitski trg 4, Zagreb, Croatia
Together with Mimara Museum, Gradec and the Lotrscak Tower, Klovicevi Dvori works as part of the Museum Gallery Centre, as exhibition premises of a…3
Art PavilionPlaces To Visit GalleriesTrg kralja Tomislava 22, Zagreb, Croatia
This Pavilion was one of the first pre-fabricated buildings in Europe. Its iron construction, made for the Millennium Exhibition in Budapest…2
Modern GalleryPlaces To Visit GalleriesAndrije Hebranga 1, Zagreb, Croatia
The Modern Gallery in Zagreb is the richest and the most important collection of the Croatian art of the 19th and 20th centuries. Its collection…2
Strossmayer Gallery of Old MastersPlaces To Visit GalleriesTrg N. S. Zrinskog 11, Zagreb, Croatia
The Gallery, together with the neo-Renaissance palace in which it is located, opened in 1884. Bishop Josip Juraj Strossmayer, who donated his…
Student Centre GalleryPlaces To Visit GalleriesSavska 25, Zagreb, Croatia
The Zagreb University Student Centre Gallery was founded in 1962. It is traditionally oriented towards exhibitors of the younger and…
Ulrich - Likum GalleryPlaces To Visit GalleriesIlica 40, Zagreb, Croatia
The Ulrich Gallery is one of the oldest galleries in Croatia. It was founded by the Ulrich family as a workshop for picture framing, and later grew…
AGM - Art Point CentarPlaces To Visit GalleriesGunduliceva 21, Zagreb, Croatia
The Gallery organizes exhibitions, sales of original prints, monographs and books, the sales of art materials and the presentation of individual…
Ulupuh GalleryPlaces To Visit GalleriesTkalciceva 14, Zagreb, Croatia
The ULUPUH Gallery is the gallery of the Association of Artists of the Applied Visual Arts. In its exhibition work, the Gallery covers various…
Amadeo GalleryPlaces To Visit GalleriesBasaricekova 18, Zagreb, Croatia
The Amadeo Gallery is an exhibition and sales gallery with a tradition of more than 20 years. In recent times the gallery has via the…
Atelier Gallery ARHOPlaces To Visit GalleriesTkalciceva 13/II, Zagreb, Croatia
The exhibition gallery of the permanent exhibition of "Mirror of Fancy" by the artist Josipa Camber. The exhibits are wall sculptures on the border…


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London, Aug 14 : A Brit couple ended up having sex in a phone booth after bad weather forced them to take shelter in it.
Despite, the booth being outside a magistrates' court and a police station, with a security guard on duty nearby, they couple romped uninterrupted for 45 minutes.
Passers-by stared, laughed and took snaps in Brighton, East Sussex - with the woman even smoking a cigarette as they romped.
"He'll probably be really proud about this. He doesn't care what people think and this is just the kind of thing he'd see as funny," The Sun quoted a pal of the man, said to be a jobless 23-year-old called Ben, as saying.
An onlooker said: "When the rain stopped they came out and just strolled across to the pub like nothing had happened."
Police said that they had received no complaints and were not trying to trace the couple.


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There could be a number of reasons why your central heating or hot water isn't working. It is possible that you can put right several problems yourself, avoiding taking time out or the expense of calling out a heating engineer. Try these central heating tips first:
1. Double check the gas, electrical and water supplies to the appliance are turned on. Somebody could have accidentally turned it them off in error, It's surprising how easily they can get accidentally switched off.
2. Check the thermostat is turned up and the clock timer is on. Maybe you need to change the battery if it is not hard wired.
3. Some combi and system boilers have a pressure gauge showing system water pressure, this usually needs to be at around 1 bar, check your boiler instruction manual if you are not sure.
4. Do you have a boiler system with a permanent pilot light? Check that it hasn't gone out – this is a very frequent issue with older boilers.
5. Try putting your central heating on maximum for a short while to see if it will kick start the boiler back into action.
6. Have the clocks gone forward or back? Perhaps it is as simple as your clock programmer might just need adjusting to the right time.
7. Has there been a power cut and a break in the energy supply? Your heating clock programmer potentially has been re set back to the original factory settings when the electric came back on. Test the central heating by setting it to come on in 15 minutes time – if that works, simply re-enter your preferred central heating settings.
8. Do you have a customer-operated reset switch (not one that needs you to remove any casing. Removing the boiler casing should only be carried out by registered Gas Safe engineers)? These are usually found on the front of modern boilers: check your user manual to see if it needs re-setting.
9. Try turning the electrical supply to the boiler off and on – the switch is usually near the boiler or in the airing cupboard. This might reset your boiler and resolve the problem.
10. Have your pipes frozen? In periods of prolonged, cold and freezing weather, the condensate pipe of your boiler can freeze.
11. The problem with central heating programmers, timers or room thermostats is that often they are overly complicated, so check your instructions and make sure that they're all set for operation. Some controls send signals remotely and can stop working, so check these too. All it takes is one wrong button pressed and no heat or hot water, so a quick battery check can solve this.
If you've carried out all these checks and your central heating still isn't working, Tempo Saves Energy would be delighted to help you. We'll first carry out a boiler system and central heating diagnosis and give you a quotation to carry out a boiler repair or if required a boiler replacement quote. A member of staff will talk you through your options, tailored to your individual needs.
• All our plumbing and heating engineers are highly trained and Gas-Safe registered
• Our engineers carry identification and wear full uniform at all times
• Prior to visiting, engineers will call to confirm an estimated time of arrival
• We only use the best products such as Vaillaint and Worceter Bosch
• No compromise on workmanship, engineering quality or customer service
• We cover all of North Wales, Cheshire, Doncaster and the wider Yorkshire areas.
I very rarely leave reviews, but on this occasion I felt I just had to.
Quite simply, Tempo ( Doncaster) were brilliant – did exactly as stated on their vans – free boilers. Turned up on time, did a fantastic job fitting my new oil boiler, no hassle, and free. Absolutely brilliant… I'll spread the word Tempo.
Thanks again.
Read case study
To Andy and staff, Many thanks for all your help today, in my emergency." Andy and the team were able to quickly respond to a boiler repair emergency in Prestatyn.Read case study
We are over the moon with the boiler install and the work that has been completed at the property. I was very happy with how professional and tidy they were and can't thank them enough for everything they have done.Read case study
© Tempo Saves Energy Ltd 2017 - All rights reserved | Website by Cloud 10


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Back when records were state of the art, the pre-Jeep Willys CJ3B was king of the jungle trail. Unfortunately, its skills haven't held up as well as its looks. Loving its style but not its capabilities, Icon Motors produced an aesthetically faithful update.
Icon Motors CJ3B
Every light is LED—from the dashboard to the headlamps. At just 64 inches wide, it's narrow and capable enough to tackle trails that Jeeps aren't technically allowed on. Just try to scratch the paint: We drove through a big-ass thorn bush and wiped away the evidence with a rag. Attracts more thumbs-ups than the Colosseum. Only 83 grand.
Only 83 grand?! Just like the old CJs, high-beam switch is next to the clutch. (They moved that for a reason.) Shifter feels sloppy, er, retro. Seat belts were a little janky, prone to locking up even in the rare moments when we had the Icon sitting level.
How We Rate
- 1/10A complete failure in every way
- 2/10Sad, really
- 3/10Serious flaws; proceed with caution
- 4/10Downsides outweigh upsides
- 5/10Recommended with reservations
- 6/10Solid with some issues
- 7/10Very good, but not quite great
- 8/10Excellent, with room to kvetch
- 9/10Nearly flawless
- 10/10Metaphysical perfection
OK, update may be understating it. Severely.
Icon acquired a few old CJs and laser-scanned all of their surfaces, generating a 3-D model of the 70-year-old vehicle. Then, they pressed new body panels out of modern steel (the old Willys was made of wartime metals that aren't as tough as modern stuff) and coated it all in a Teflon-polyurethane powdercoat that we couldn't scratch despite hurling the truck at trees instead of around them.
While the outside looks just like an old Willys, a 1940s mechanic would never recognize the undercarriage. The new, mandrel-bent frame is designed to accommodate a fully independent suspension, instead of the old leaf springs. The Chevy-sourced 4-cylinder engine makes 205 horsepower, but gets 23 mpg.
The 4WD system is adapted from a 2010 model-year Jeep: It's more advanced, and, as an added bonus, owners won't have to scour eBay for antique replacement parts when they get overambitious in the off-road park.
And while a 1950s 3B tops out at around 50 mph on the highway, we throttled the Icon down L.A. freeways upwards of 80. Then, when we hit the Miller Jeep Trail, we switched into 4WD low and went way beyond our comfort zone without seeing the Icon balk. With 24 inches of wheel travel, it can scurry over boulders, romp rutted trails, and negotiate inclines that would make a Hummer run screaming for a pedicure.
When you buy something using the retail links in our product reviews, we earn a small affiliate commission. Read more about how this works.Go Back to Top. Skip To: Start of Article.


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Next Episode of Have Gun, Will Travel is
not planed. TV Show was canceled.
The man known only as "Paladin" lives in San Francisco at the Carleton Hotel, accustomed to the fanciest food and furnishings. He afford it all by riding out as a gunslinger and charging top dollar for his services. His calling card? "Have Gun Will Travel." Paladin will take on any case if a client meets his fee, but whether he helps them or not depends on whether he thinks that they deserve it. A West Point graduate with an unknown past, Paladin is as likely to help a client with his lightning wits and sense of strategy, as he is to use his gun and his hold-out derringer.
Paladin rides into a small Texas town and finds a young couple in need of a room so that the wife can deliver her body. However, the only saloon in town is filled up with drunken cowboys celebrating Christmas Eve and having no interest in helping.
Richard Boone(Paladin)
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Editors' Note: The download button opens the iTunes App Store, where you may continue the download process. You must have iTunes installed with an active iTunes account in order to download and install the software. This download may not be available in some countries.
|Entertainment Software
Publisher's Description+
From App Manufacture: New: Comes with iPad HD support included! Please compare! This is the only phone tracker that offers support for all versions of iPhone, iPod Touch and iPad! Mobile Locator lets you track mobile phones from any provider using the latest GPS location technology - as long as the other mobile phone is very close to you! :-)Be aware: This is the best joke ever! Show the app to friends and tell them you are able to locate their phone. Enter the number and show them how the app locates them. The will be scared as hell. Of course the app cannot locate other phones but only yours. However, as your friend is standing next to you for this joke it is guaranteed to work 100%.
What's new in this version:
iPhone 5 screen supportSpeech output for tracking state
read more +


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Assuming a small enough handgun and room in the pocket, nothing beats the convenience of pocket carry. It's no more trouble than putting a wallet or a cell phone in your pocket.......an absolute no-brainer, and that's the allure for most.
Tucking IWB can be a bit of a pain, but is much better suited to a larger handgun. I pocket carry my Seecamp. I carry IWB, either tucked or untucked my Kahr K9. If I'm not already carrying and just want to run down to the corner store for some milk, I'll drop the Seecamp in my pocket every time.


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A Father Asked Facebook to Make a 'Look Back' Video for His Dead Son . . . and They Said Yes [Video]
You know those one-minute 'Look Back' videos Facebook made everyone earlier this week, to celebrate their 10th anniversary? Well, a father named John Berlin wanted them to create one for his son Jesse, who passed away in January of 2012.
He says HE can't access his son's site, but Facebook COULD. So he posted an emotional plea on YouTube yesterday, personally asking Mark Zuckerberg and Facebook to do it. And they said YES. If you watch his video, you'll see why.
And under the video it now says: "Facebook contacted us and they're going to work on that vid. I want to thank everyone, for your support. I didn't know what to expect from this but it was worth a try. My son was 21 and died of natural causes . . ."
"He is proof that there are no guarantees in life. Hug your children . . . kiss them. Tell them you love them every single day. Again I thank every single one of you, even the dislikes. God Bless."
Kane is joined by co-hosts Riley, Rose, Intern John and producer Erick. The high-energy, fast-paced program features music, conversations on the latest news and hot topics, Hollywood news, contests, call-in interaction with listeners, plus interviews with the biggest celebrities in music and pop culture. The show features signature segments such as "War of the Roses," "Hollywood," "The Tea," "John's Justice," "Ghosted" and "Moron Madness."
Kane Cares! We'd love to hear about your event or organization. Use this link to send us your information!


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Incredible Christian Remix Of 'Let It Be' Gave Me Chills
Description:There is beauty in righteousness by faith. And this stunning remix of 'Let It Be' had my hands in the air with praise. This is just the lift I needed today!
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Check out bb3.ca's January newsletter and even better – Amethyst Earrings Giveaway. They are some of my fave earrings as this lavender amethyst is so delicious. Although you can choose surgical steel or sterling silver ear hooks, the giveaway is for the sterling silver version.
Don't forget to join me on Facebook for more chances to win. Giveaway is open for all newsletter subscribers and facebook followers. I'm taking entries until January 31st, 2017 (midnight EST).
Good luck and thanks for entering!
Leilani Cleveland Deveau is a self-taught jewelry artist with over sixteen years of experience. 4 years ago she added the fiber arts (wool processing/dyeing/carding/spinning, knitting, crochet and felting) to her long list of creative interests. Leilani welcomes questions or comments from other crafters and handmade artists, as well as requests for custom, wholesale or consignment jewelry. Her home studio is located in the Annapolis Valley, Nova Scotia. You can contact her by email: firstname.lastname@example.com


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Local News
Lodging tax revenues for November improve-
Lodging tax revenues for the eleventh month of 2012 were up compared to November of last year county-wide.
North American Odyssey continues down east coast-
Dave and Amy Freeman of Cook County are kayaking the inland waterway off the east coast as they head into the final stages of their North American Odyssey.
Cooler but normal weather this week-
Looks like a chilly News Years with a chance of snow, then a warm-up. WTIP's Jay Andersen spoke with meteorologist Mike Stewart about holiday weather.
Weekend News Roundup for December 29-
Each weekend WTIP news produces a round up of the news stories they've been following this week.
Lonnie Dupre returns to Denali for a third attempt-
Grand Marais adventurer Lonnie Dupre is back in Talkeetna Alaska preparing for this third attempt to conquer North America's highest mountain...alone and in the dead of winter.
Back to school after what may have been a stressful holiday for some-
There's been a lot of disturbing news on TV over the holidays and that can have an effect on school-age young people.
Master Naturalist trainings at Sugarloaf Cove-
Sugarloaf: The North Shore Stewardship Association is pleased to offer Master Naturalist volunteer training at Sugarloaf Cove.
Winter stream trout fishing in BWCAW to open Dec. 29-
A 2012 Legislative change in fishing regulations will allow winter stream trout fishing on all lakes entirely within the Boundary Waters Canoe Area Wilderness to open earlier, according to the Minneso


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Event: Salman Khan, Kareena Kapoor, Atul Agnihotri, Aditya Pancholi unveiled the first trailer of their upcoming film BODYGUARD.
Guest List: Kareena Kapoor, Salman Khan, Aditya Pancholi, Shera, Siddique, Atul Agnihotri, Alvira Agnihotri, Arpita Khan
Venue: PVR, Juhu, Mumbai
Date: 21-Jul-2011
Bollywood actor Shahid Kapoor's brother Ishan Khattar was snapped at PVR, Andheri.
Bollywood celebs Tiger Shroff and Nidhhi Agerwal were snapped promoting their upcoming film MUNNA MICHAEL.
Bollywood actor Salman Khan's friend Iulia Vantur was snapped at the special screening of TUBELIGHT.
Bollywood badshah Shah Rukh Khan was snapped at the special screening of TUBELIGHT. Also spotted was director Imtiaz Ali.
Bollywood actoe Shah Rukh Khan's daughter Suhana Khan watches Salman Khan's TUBELIGHT at the special screening.


text:
The Competitiveness of G20 Nations
Edited by: Li Jianping, Li Minrong, Zhao Xinli, Li Jianjian, Huang Maoxing, Su Hongwen
Innovation is the eternal theme in human and social development; it is the soul for a nation to advance and an inexhaustible driving power for a country to thrive. -- Preface, The Competitiveness of G20 Nations *** Written and edited by some of China's leading economic advisers and government officials, this unique book reveals exactly how China views the competitiveness of the world's leading nations. Thoroughly analyzing detailed research material, the book probes the innovative competitiveness of each member of the G20 and their future prospects. The Competitiveness of G20 Nations argues that innovation is at the core of the growth prospects of each member of the G20 - an approach that reveals some of the theories and reasons behind China's stratospheric economic growth. Readers will benefit from a detailed innovation profile of each G20 nation (including China), highlighting the perceived strengths, weaknesses, and the likely growth patterns. (Series: China International Analysis and Evaluation Reports)
278 pages
Publication Date: 2/1/2013
Format: Cloth


text:
Presidential, #Romney, #debate2012
Last night's presidential debate has swung public opinion in favour of Republican challenger Mitt Romney, who, according to today's IV Drip, "appeared feisty and combative in contrast to a more measured (some would say weedy) Obama."
Read the round-up of reactions from the US newspapers here. And, to see what celebrities have been tweeting about it, click here.
@OliviaWilde tweets: "Mittens, if you want the federal gov't out of our health care decisions, then stay the F out of our VAGINAS. #debate2012."
London's Piccadilly Lights are being emblazoned with lines of poetry to celebrate National Poetry Day, today, now in its 18th year.
Getting into the spirit of things, poet and journalist Musa @Okwonga tweets: "Dad, I outgrew you - I six-foot-two'd you!/Touché, you lose, at six-foot-one.."
Turkish artillery has fired on Syrian targets for a second day, Turkey's state-run media said, in retaliation for shelling from Syria that struck a border village, killing five civilians. @Reuters tweets: "Syria says it respects the sovereignty of neighboring countries, urges "states and governments" to act wisely and rationally".
#Dave #smeghead #RedDwarfX
After a very long wait, Red Dwarf is finally returning to our screens, on Dave tonight at 9pm.
It looks like the cast have been marauding around London to celebrate the return of the series. A return to form, then, as star Robert Llewellyn
revealed in a recent interview with the Sun, "When I started in 1989, [getting the makeup done] was a five-hour job, so I got up incredibly early. As I was going down in the lift at 4.30am, Craig would be coming back from partying."
Red Dwarf fan Kate tweets: "It's #RedDwarfX day, that means DannyJohnJules back on my tv. I'm a happy lady".
Follow Emily JuppReuse content


text:
Analysis of a Cylindrical Shell Vibrating in a Cylindrical Fluid Region
Description: Analytical and experimental methods are presented for evaluating the vibration characteristics of cylindrical shells such as the thermal liner of the Fast Flux Test Facility (FFTF) reactor vessel. The NASTRAN computer program is used to calculate the natural frequencies, mode shapes, and response to a harmonic loading of a thin, circular cylindrical shell situated inside a fluid-filled rigid circular cylinder. Solutions in a vacuum are verified with an exact solution method and the SAP IV computer code. Comparisons between analysis and experiment are made, and the accuracy and utility of the fluid-solid interaction package of NASTRAN is assessed.
Date: August 1976
Creator: Chung, Ho; Mulcahy, T. M.; Turula, P. & Jendrzeczyk, J. A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department


text:
- Score:
- rated 4.40 / 5 stars
- Views:
- 6,769,845 Views
- Share Links:
- Genre:
- Action - Platformer - Puzzle
- portal
- valve
- portalhistory2007
Credits & Info
- Uploaded
- Oct 9, 2007 | 10:45 PM EDT
- Frontpaged October 10, 2007
- Weekly Users' Choice October 17, 2007
- Daily Feature October 11, 2007
If you liked this, check these out!
Plenty more like this here!
Author Comments
Rated 5 / 5 stars
great game and also here is a song that you can continue above me or reply to this commen
This was a triumph
I'm making a note here: HUGE SUCCESS
It's hard to overstate my satisfaction
Aperture Science
We do what we must because we can
For the good of all of us
Except the ones who are dead
But there's no sense crying over every mistake
You just keep on trying till you run out of cake
And the science gets done
And you make a neat gun
For the people who are still alive
I'm not even angry
I'm being so sincere right now
Even though you broke my heart and killed me
this is the website to continue the song
Rated 4.5 / 5 stars
Really nice job for a remake.
Rated 4.5 / 5 stars
I think I broke the game on lvl 9. But this is a spectacular game. This is better than portal.
Rated 5 / 5 stars
This game is enjoyable and challenging :)
Rated 3.5 / 5 stars
Not really a fan of this game but good work anyway.


text:
For U.S. architects, working abroad will now be easier than ever, as the National Council of Architectural Registration Boards (NCARB) has announced a new mutual recognition arrangement with the licensing authorities of Australia and New Zealand. Effective as of January 1, 2017, the agreement allows architects to earn reciprocal licenses that authorize architects to work in the two countries.
New Zealand Architecture
Latest projects in New Zealand
Latest news in New Zealand
New Agreement Allows U.S. Architects to Earn Reciprocal Licenses in Australia and New Zealand
2016 New Zealand Architecture Awards Announced
The New Zealand Institute of Architects Incorporated has announced the winners of the 2016 New Zealand Architecture Awards, which recognizes the best works by New Zealand's architects.
See 2015's Best Public Toilets From Around the World
Now in its second year, DesignCurial has revealed the results of their annual "Top 10 Public Toilets" award, recognizing outstanding lavatory design across the world. The top 10 emerged as exemplars among over 1,000 public toilet designs, evaluated on "design, innovation and the relationship with surroundings". "The aim of the list was to bring international design to the fore, especially unique architecture that may be overlooked due to the structure's mundane stereotype," said Katherine Houston, web editor at DesignCurial. "This list is well geographically spread, with distinctive designs emerging from Japan, Australia and the UK to name a few." See the world's best public toilets for 2015 after the break.
2015 New Zealand Architecture Awards Announced
The New Zealand Institute of Architects has announced the 28 winners of the New Zealand Architecture Awards during an event at the Auckland Museum.
Studio Dror Designs Steel Sculpture for New Zealand Winery
Studio Dror has been commissioned by Brancott Estate in New Zealand to design a steel sculpture inspired by the winery's history. The result, "a flat matrix of CorTen steel" that "stretches open to become a nine-meter-tall volume of aggregated triangulations." Named "Under/standing," the installation was inspired by the winemaking process. It will be built in 2016.
Hundertwasser's Last Unbuilt Work Could Become a Reality in New Zealand
The future of Friedensreich Hundertwasser's last unbuilt work -- a proposed Art Center for Whangarei, New Zealand -- hangs in the balance and will be decided next week by a referendum. The center, backed by the Proper Northland Trust (PNT), was originally designed in 1993 by Hundertwasser to repurpose a waterfront government building, but was never completed.
Winners of the 2015 Auckland Architecture Awards Announced
From 159 submissions, 44 have emerged as winners of the NZIA's 2015 Auckland Architecture Awards. The titles were bestowed upon 20 firms for new projects spanning 10 categories, ranging from a restrained renovation of a historic building in a Victorian neighborhood, to a bold, modern transportation hub. This year's awards were grounded in three main areas of the city, with Britomart dominating in hospitality and retail designs, Hobsonville Point receiving educational and multi-unit housing awards, and Titirangi gaining recognition for its stellar public architecture and housing.
25 of New Zealand's Best Buildings Receive 2015 Canterbury Awards
With a record number of entries, the 2015 Canterbury Awards, organized by the New Zealand Institute of Architects (NZIA), honored 25 Canterbury-based designs spanning 10 categories. The esteemed awards recognize and promote projects that are exceptionally sensitive to both their environment and occupants. Prevalent in this year's awards was the theme of rebuilding, as several projects were realized despite earthquakes impeding their construction, resulting in innovative designs that adapted to the unforeseen setbacks.
Shigeru Ban on Growing Up, Carpentry, and Cardboard Tubes
He may have risen to prominence for his disaster relief architecture and deft use of recyclable materials, but Shigeru Ban describes his idiosyncratic use of material as an "accident." Speaking to The Wall Street Journal, the 2014 Pritzker Prize Laureate recalls turning to cardboard tubes as a matter of necessity. "I had to create a design for an exhibition," Ban told the newspaper, "But I couldn't afford wood. Instead, I used the many paper tubes from rolls of drafting paper that were lying around. The tubes turned out to be quite strong." The most prominent of Ban's cardboard tube structures is Christchurch's Cardboard Cathedral, built in the aftermath of an earthquake that devastated the city in early 2011. Read WSJ's full interview with Ban here.
Red Architecture Wins Top New Zealand Prize for "Innovative Black Barn"
Red Architecture's "innovative black barn" has been awarded the ADNZ's (Architectural Designers New Zealand) 2014 Supreme National Design Award for its "subtle, economical and clever design." Located in the beautiful rural landscape of Whatawhata in the Waikato, the project houses a private residence and garage within two "crisp barn-like forms" clad in vertical run steel and recycled bricks taken from the devastation caused by the Christchurch earthquakes.
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text:
Whistleblower Cases Hit New RecordsKaren Aho
The federal government is on track to receive record payouts this year for contractor-fraud lawsuits, and it's all thanks to whistleblowers—workers more apt to lose their jobs than win big cash settlements.
Following a $2.2 billion payment in November by Johnson & Johnson for its alleged off-label use of Risperdal and other drugs, the Department of Justice should collect more than $5 billion under the federal False Claims Act by the close of fiscal year 2014, said Patrick Burns, co-director of the nonprofit Taxpayers Against Fraud Education Fund.
That may be a drop, compared to what the feds are owed—including some $300 billion in unpaid taxes—but it's a significant jump over the $86 million the government collected in 1987, the year it decided to significantly boost payouts to whistleblowers in an effort to fight fraud. That year, 30 citizens filed cases qui tam, a term stemming from the latin for "he who sues in this matter for the king, as well as for himself." In 2013, a record 753 did, making whistleblowers responsible for 89 percent of DOJ fraud cases filed that year.
These days, health-care fraud makes up the bulk of DOJ's cases, and whistleblowers receive 16 percent of the settlement amount, on average. This has helped the government collect $39 billion since 1987 ($55 billion, if you include criminal fines). Whistleblowers have earned $4.3 billion, including $388 million in 2013.
All this comes at great risk to the whistleblowers. Lawyers say the DOJ fails to join in on roughly three-quarters of qui tam cases, leaving little chance a law firm will continue on its own. Even if a suit is one of the 150 or so per year that reach a favorable settlement, payouts are rarely "life changing," said lawyer Erika Kelton, of Phillips & Cohen, in Washington.
Few whistleblowers land like the Alabama nurse who was paid $15 million in April for reporting alleged fraudulent Medicare billing. Typically, "whistleblowers still have to work," said Kelton; in spite of federal protections, many end up blacklisted in their professions. Among her clients: a senior engineer who ended up mowing lawns and bagging groceries and a senior pharmaceutical sales rep who took to painting houses.


text:
Cheetah is ranked number 23,098 in the overall artist rankings with a total rank score of 2.
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|Rock And Roll Women
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WWII 1st Day Cover NO CANCELS
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4 Posts
Posted 12/12/2016 6:30 pm
Hello to all, newby here. I recently picked up a box of Stamps and related items at a swap meet. There were some 1st day covers with this one among them. I noticed that it had a stamp attached, but not cancelled like the others. The cover features a Submarine, The USS AMBERJACK. I have spent several hours on the internet. I found lots of information about the Sub. (It was lost in 1943 with all on board), but I could find nothing about this cover. Any help would be greatly appreciated.
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Posted 12/12/2016 6:51 pm
Well, a picture is worth a 1000 words, somebody once said . . .
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regards, TR
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Posted 12/12/2016 7:45 pm
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United States
162 Posts
Posted 12/12/2016 7:58 pm
Wow, look at the recipient in the second FDC!
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United States
4 Posts
Posted 12/12/2016 9:22 pm
Thanks for the response folks. I have been trying diligently to post a picture, but my Irfan program seems out of whack. The cover I have is unlike any that has been posted. I have not been able to find another copy and I have been all over the internet. I will keep on until I can get the picture posted. Here is a little more info while I am trying to post the picture. The design and lettering is all in "raised" or embossed silver ink or whatever. A name and address is also stamped in the lower right, but it obviously never went thru the mail.
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Posted 12/12/2016 9:43 pm
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605 Posts
Posted 12/13/2016 02:50 am
Wow, look at the recipient in the second FDC!
Roosevelt was well known as a stamp collector and so he was deluged with such mail from well meaning collectors. I have no doubt that he never saw much less handled 99 percent of what was sent to him. Every President gets warehouses full of "stuff" sent to them from well meaning people and that they never see. Most of it gets sold off or just trashed at some point. Much of Roosevelt's "collection" of stamps and covers including this sort of thing was sold by his family at a series of four big auctions by Harmer, Inc. where there were many huge box lots sold. Another part of it ended up at the Smithsonian. There are also covers that were addressed to him but never mailed as they were cancelled and handed back to the person who prepared the covers. You can do this even today and address a cover to whomever you like but not actually send it to them.
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Edited by Kimo - 12/13/2016 02:59 am
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Posted 12/13/2016 02:54 am
First day cover makers, as well as just plain Naval cover makers who made covers every time a Naval vessel stopped in a port or participated in an exercise, or sometimes just when the captain sneezed, sometimes had left over covers they did not fully process for various reasons. Such covers today have little to no value as most all collectors really do want the cancellation to go with the rest of the cover.
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Posted 12/13/2016 8:48 pm
Thanks to redwoodrandy for the links. I have learned a lot about covers in a short time. Thanks also to KIMO for your explanation. I can see how true believers would want the cancellation data on the covers. My real interest has always been coins. I should have passed on this box of stamp material as I am finding that one can burn up a lot of hours doing research on something he knows nothing about with very little reward.
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Posted 12/15/2016 12:08 pm
Too bad it was not cancelled as the cancellation is really important to these, though even if it were cancelled the value would still be somewhere between $1 and $5 depending on how badly a collector needed this particular one for their collection.
This one has a nicely made cachet in silver colored ink. I do not know the name of the maker of the cachet though. Such cachets were all privately made and are not official - there were a great many makers of them and all tried to make theirs unique and fancy since most the makers of the cachets were either stamp dealers who would get a bunch of their cacheted covers canceled by the guy on the boat or ship whose duty was to handle the incoming and outgoing mail for the men, or the maker of the cachet would sell blank covers with his cachet to collectors for them to send off to get the on board cancellation.
Here is an example of another first day of postal service cachet that seems to have been made by the same cachet making company as yours, only this one is for a different submarine, has slightly different wording and was printed in gold colored ink. This one was properly cancelled making it of interest to collectors. It would be valued in the range of $3 to $4.
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New Member
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4 Posts
Posted 12/15/2016 10:27 pm
Kimo, thanks for the detailed explanation as to how some of these covers came about. In the box,I found another by the same person. This one is for the Keel laid for the USS Currituck-AV7. It too has a stamp in place, but no cancel. There is a name in very small print on the rear bottom right. It reads: S.E. Hutnick M.D. 5933 Lansdowne Ave. West Phila., Pa.
By the way, the he one you picture is most certainly made by the same person or company. The style is a give away as is the R.C.D. 70 at the bottom. I do not know the significance, but it is on both of the examples that I have.
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Posted 12/15/2016 11:17 pm
Dr. S.E. Hutnick M.D. cachet maker
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Posted 12/15/2016 11:21 pm
Naval Cover Museum
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Posted 12/16/2016 01:00 am
There is a group of collectors of U.S. Naval covers called the Universal Ship Cancellation Society and their website is at
Cancellations and commemorative covers from US Naval ships go back to the first world war, though you can find mail from US ships from before then even. I do not know how many different ones there are considering that every US Navy ship or boat had special cancels and starting the in the 1930s there was a real craze for making special cachets and inserting interesting information in the killer bars for all kinds of events and there were many different companies and individuals making cachets for such covers. The result is that there must be many tens of thousands of different ones to collect. As such, most collectors of these tend to specialize either in a kind of ship or boat such as just submarines or just air craft carriers or just battleships, etc., or just covers that were produced in a single country or port whenever a US naval vessel paid a call there, or just a given decade, or just one or two cachet makers' work, or whatever a person fancies as being fun to collect. The possibilities are endless, the covers are mostly very affordable, and they are very plentiful and easy to find. It is a great collectable for anyone with any budget and it is fun to learn about the ships, what they were doing and where in the world were they going.
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The vendor, LovelySkin was awesome. The free samples they offered were terrific, too. However, the new version of what used to be Colorscience Skin Calming Primer is not as good as the original! I don't like that scent has been added. I am also not as happy with the consistency of the new product. I'd say, bring back the original Skin Calming Primer.
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I have very oily skin and this product has really helped. It creates a smooth foundation for my other products. Keeps my shine free for many hours.
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I have been a faithful user of the Skin Brightening Face Primer SPF 20 for many years. I decided to give the Mattifying Primer a try, and am sure glad I did. This works great under my mineral powder make up and helps keep the oil slick at bay.
Previously I was using smashbox primer but wanted one with sunscreen. I like this product but I usually wait about 10 minutes before applying make up
This primer goes on nicely and feels very light on the skin, but it makes the application of liquid foundation a frustrating and difficult task. It almost feels as though you are applying foundation on a glue-like surface, rather than a smooth surface that other primers produce. Using this primer wastes more time on makeup application, and also wastes more foundation in the process. And although it produces a matte finish, it is no more mattifying than other primers on the market. It is so "sticky" that when I blot my face with tissue to soak up oil, minute remnants of tissue get stuck to my face. Perhaps this primer is better-suited for those who wear loose mineral and powder foundations.
A little goes a long ways! This primer made my makeup last all day, kept the greasies at bay, and smelled great. No breakouts, didnt feel pasty like some primers do. Highly recommend!
So far so good...its really does mattify the face. Also makes it incredibly smooth for applying makeup. I dont notice as much oil on my face come the afternoon, so no shiny face anymore. Cant say it helps with blemishes much, unfortunately, I have very hormonal adult acne and little to nothing helps with that. But it is nice and does give my face a slightly softer look and feel.
A bit pricey for a primer, but works like a charm, covers imperfections nicely and leaves a nice canvas to work with for apply makeup.