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Katy Perry Killer Queen 30ml EDP Gift Set
From £1.50 per week
- Price
- £30.00
- In Stock
5 reviews
- Delivery
- Next day delivery available
Katy Perry's new Killer Queen fragrance comes in a stunning gift set that's sure to impress.
This strikingly gorgeous Floriental scent comes in a stunning bottle inspired by a queen's sceptre, and the gift set also includes 75ml body lotion and 75ml shower gel.
With its jewel-shaped bottle and crown stopper complete with cat, musical clef and dagger motifs, the Killer Queen bottle is perfect for any dressing table!
Top notes of bergamot, wild berry and dark plum evolve into a heart of red velvet flower, rainbow plumeria and jasmine, rounded out by base notes of patchouli, cashmeran and liquid praline.
Katy says: "I was really inspired by the idea of a strong, confident woman in power and that is exactly what I hoped to put into this fragrance, something that brings out the power inside of you."
Useful info:
- 30ml perfume, 75ml body lotion, 75ml shower gel
- Perfume comes in stunning red crystal bottle
- Beautiful presentation box
- Katy Perry Killer Queen 30ml EDP Gift Set
If you're looking for a sophisticated everyday scent for him, look no further than Ted Baker M 30ml EDT and 2 Body Washes Gift Set.
Refreshing and clean, Ted Baker M for him EDT is a sophisticated aftershave with oriential tones.
Ted Baker M opens with sparkling notes of Japanese yuzu, roseberry and black peppercorn before evolving into heart notes of coriander, elemi oil and guaiacwood resin, rounded out by a smooth base of vetiver, tonka bean and grey musk.
The gift set includes 2 fragranced body washes so that he can start each day as he means to go on.
Useful info:
- 30ml EDT, 2 body washes
- Great gift idea
- Comes in a stylish presentation box
- Ted Baker M for him EDT and 2 body washes gift set
Lady Gaga Fame 50ml Gift Set
From £2.00 per week
- Price
- £40.00
- In Stock
4 reviews
- Delivery
- Next day delivery available
The Lady Gaga Fame 50ml Gift Set is the perfect way to unleash your inner little monster.
The first of its kind, the black-to-clear perfume is an innovation in fluid technology, transforming from black to transparent when it touches you skin. The concept reflects Lady Gaga's ideas about the benefits and darkness that come with fame.
Not many perfumes come with their own promo video – but then Lady Gaga's Fame EDP is no ordinary scent. Gladiator director Ridley Scott produced a remarkable video that has given Lady Gaga's new perfume a massive global publicity push.
The 50ml perfume has a floral flagrance full of dark passion and intrigue. Inspired by the deadly nightshade belladonna, it was developed using a 'push me-pull me' concept that allows allows notes of incense, apricot, tiger orchid, saffron, honey and jasmine to emerge as you wear it.
This set also includes 2 luxurious back fluid shower gels that complement the passionate and intriguing scent of the perfume to really satisfy your inner monster.
Useful info:
- First black-to-clear eau de parfum
- Includes 2 shower gels
- Perfect present idea
- Comes in a stylish presentation box
- Cocoon bottle with cap inspired by Monster Claws
- Lady Gaga Fame 50ml Gift Set
Ted Baker W 30ml EDT Ladies Perfume with 2 Body Lotions Gift Set
Refreshing and clean, Ted Baker W For Her EDT is a versatile scent suitable for all occasions.
Ted Baker W opens with top notes of lush fig accord, white peony and African violet, evolving into heart notes of pink orchid, cassis and raspberry, rounded out by smooth base notes of cedar and vanilla.
This gift set is presented in a pink box and contains the EDT along with 2 scented body lotions for top to toe luxury.
Useful info:
- 30ml EDT and 2 body lotions
- Great gift idea
- Comes in a stylish presentation box
- Ted Baker W EDT ladies perfume with 2 body lotions gift set
Bench Mens Remix 100ml Gift Set
From £1.40 per week
- Price
- £28.00
- In Stock
2 reviews
- Delivery
- Next day delivery available
For a modern man with a city lifestyle Bench Urban Original 100ml EDT and Shower Gel Gift Set is the ideal present.
Emphasising modern masculinity, the aromatic fragrance of Bench Urban Original is a complex scent that combines citrus and fruit aromas with spicy botanicals.
Top notes of aniseed, bergamot, lemon, melon, apple and violet give way to a warming heart of grapefruit, jasmine, lavender and rose, with a base of patchouli for a contemporary men's fragrance.
The gift set includes a shower gel for top to toe fragrance.
Useful info:
- 100ml EDT, shower gel
- Great gift idea
- Bench Urban Original EDT and shower gel gift set
Issey Miyake L'Eau D'Issey 50ml EDT Gift Set
From £0.94 per week
- Price
- £70.00
- In Stock
1 review
- Delivery
This Issey Miyaké L'Eau D'Issey EDT Gift Set features a fresh and fun fragrance, along with a gorgeous shower cream and a luxurious body lotion.
The iconic fashion brand is known for its fresh and innovative designs and this gorgeous scent is a wonderful reflexion of this.
This aquatic floral scent boasts transparent notes of lotus, freesia, cyclamen and juicy melon. The middle notes of peony, lily, and carnation add fresh tones while the end notes of cedar, sandal, musk and amber create a warm and woody finish.
The delightful shower cream has a complementary scent that will ensure you feel refreshed and revived all day long, while the wonderful body lotion nourishes skin to leave you feeling fabulous.
Useful info:
- 50ml L'Eau D'Issey EDT
- Includes body lotion
- Includes shower cream
- Issey Miyaké L'Eau D'Issey EDT Gift Set
Jean Paul Gaultier Kokorico Mens 50ml Gift Set
From £0.72 per week
- Price
- £54.00
- In Stock
2 reviews
- Delivery
This Jean-Paul Gaultier Kokorico Mens Gift Set is a powerful fragrance for the confident, contemporary man.
Jean-Paul Gaultier is known for his bold, cutting edge style and this striking set certainly embodies this. The 50ml eau du toilette has sensual top notes of fig leaves and raw cocoa that work alongside the timeless patchouli and cedar to create a long-lasting and masculine scent.
The unique bottle has an authoritative look to complement the commanding and manly fragrance, with a face to the front that becomes a torso in profile.
This set comes with an invigorating scented shower gel to energise and enthuse. The striking presentation tin also makes it the perfect present for a man about town.
Useful info:
- 50ml eau du toilette
- Includes 75ml shower gel
- Presentation tin
- Jean-Paul Gaultier Kokorico Mens Gift Set
Contains 3 x 30ml EDT sprays, So Eternal, So Kiss Me & So Original.
Bench Ladies Gift Set
From £1.25 per week
- Price
- £25.00
- In Stock
1 review
- Delivery
- Next day delivery available
A great gift for a woman wanting a clean daytime fragrance, Bench Urban Original EDT is a fresh, sweet fragrance and she can feel good from head to toe with the matching shower gel.
Bench looked to music culture for inspiration when designing Urban Original and have come up with this edgy, earthy fragrance – perfect for the woman whose top weekend is spent at Glastonbury or V Festival.
Top notes of apple, mandarin and bergamot bend with a heart of orange blossom, magnolia, jasmine and lily alongside a base of musk and sandalwood.
The boxed gift set contains a 50ml EDT and a shower gel so that she can get that fresh scent straight from the shower.
Useful info:
- Contains 50ml EDT and shower gel
- Boxed set
- Great gift
- Clean, fresh fragrance
- Bench Urban Original EDT and shower gel gift set
The Gaultier Ladies Gallery. Contains 4 mini Jean Paul Gaultier fragrances. 2 Classique 3.5ml EDP, and 2 Classique 3.5ml EDT. Each bottle with it's own design. Perfect to pop in your bag.
For the adventurous, choose Calvin Klein Escape for Men 100ml EDT and 200ml After Shave Balm Gift Set.
For a man who pushes the boundaries of everyday life, Calvin Klein Escape is the only real option.
Escape has a distinctly Mediterranean feel with top notes of melon, grapefruit, mango and bergamot giving way to a meaty heart of rosemary, cypress, sage and birch. A warm, spicy base of sandalwood, amber, patchouli and oakmoss lingers on the skin.
The gift set is stylishly presented and includes a fragranced after-shave balm to soothe the skin.
Useful info:
- 100ml EDT, 200ml after shave balm
- Great gift idea
- Stylishly presented
- Calvin Klein Escape for Him EDT and after shave balm gift set
Lipsy Original 50ml Envelope Boxed Gift Set
From £1.60 per week
- Price
- £32.00
- In Stock
3 reviews
- Delivery
- Next day delivery available
Contains 50ml EDP and body lotion in a Lipsy Box.


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The L-Dens 427 series of density sensors combines an accuracy of 1 x 10-4 g/cm³ in a compact design. Continuous measurement of density, concentration and API gravity optimizes production processes and ensures a consistently high quality of product. The small and compact design allows for easy integration into measuring stations, for example for mass flow rate measurement.
Flowrox Launches New Industrial Instrument For Imaging Paraffin Wax And Asphaltene Depositions In Oil And Gas Pipelines
Flowrox, a global leader in heavy-duty industrial valve, pumps and instrumentation manufacturing and service, is releasing to the Oil & Gas market the Flowrox Deposition Watch—a new instrument designed to enhance the monitoring of pipelines and related flow-process equipment affected by paraffin wax and asphaltene depositions.
Export Application Filed With U.S. Department Of Energy For Alaska LNG Project
In another important step forward for the Alaska LNG project, an application to export liquefied natural gas (LNG) was submitted to the U.S. Department of Energy.
Petroleum Demand Up In June And Second Quarter
Total U.S. petroleum deliveries (a measure of demand) rose 1.5 percent from June 2013 to average just above 19.0 million barrels per day last month. For the second quarter, petroleum demand increased 1.5 percent compared to the same period last year.
API Issues 'Good Neighbor' Standards For Oil And Natural Gas Developers
API has published a first-of-its-kind industry standard for community engagement in areas of the country where horizontal drilling and hydraulic fracturing have opened new energy development opportunities.
TIW Corporation Announces The Completion And Delivery Of Two Large Diameter Ball Type Safety Valves To Marine Well Containment Company
TIW Corporation has announced the completion and delivery of two large diameter Ball Type Safety Valves to Marine Well Containment Company (MWCC) for use with its expanded containment system.
Maximising Value from Mature FieldsJuly 28 - 30, 2014
Oil and GasAsset Integrity and Inspection Management SummitJuly 28 - 30, 2014
Houston, TX
Shale & Tight Gas FundamentalsJuly 30 - 31, 2014
Informa Australia, George Street, Brisbane, 4000
The Cybersecurity for Oil, Gas and PetrochemicalsAugust 11 - 14, 2014
Kuala Lumpur
REU Houston 2014August 11 - 14, 2014
Houston, TX

subset: commoncrawl


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WI DOT Road Construction on HWY 13
The Wisconsin DOT plans to replace three culverts on WIS 13 between Cornucopia and Red Cliff. Culverts will be replaced at these locations: Saxine Creek, Saxine Tributary, and Sand River Tributary. The construction may impact your trip to Apostle Islands. More »
Current Closures
There are current closures of areas within the Apostle Islands National Lakeshore. Click for more information and see if these closures will affect your trip. More »
Otter Island
A 1.9 mile trail leads from the dock to the north end of the island.
Did You Know?
Long Island is home to the only known nesting sites in Wisconsin for the endangered Piping Plover. In 2012, five nests were successful with a total of eleven surviving chicks!


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2014 Degree Candidates
Spring 2014 candidates for Willamette University's Master of Arts in Teaching (MAT) degree are excellent prospects for teaching positions. These candidates will qualify for the MAT degree and the Oregon Initial I Teaching License after successfully completing the program in June. In this guide you will find highly qualified teaching candidates for authorization levels in Early Childhood, Elementary, Middle and High School. Please see the left navigation bar for a link to the previous year's graduates.
Finding a Job
Learn more about how to find a job. Resources for searching for jobs in Oregon, across the country and overseas are available to current candidates and graduates.
The Oregon Writing Project
The Oregon Writing Project at Willamette supports excellence in the teaching of writing at all educational levels and subjects through collaboration, education, and community involvement. Through a professional development model, OWP at Willamette aims to build the leadership, programs, and research needed for teachers to help their students become successful writers and learners.
Oregon Technology in Education Network (OTEN)
Willamette University is one of six partners in the OTEN Consortium that received a $1.6 million Teacher Quality Enhancement-Partnership grant from the US Department of Education. It is designed to increase student achievement in K-12 classrooms in high need schools and to assist teachers who use technology in the classroom.


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Matches found in Motorcycle News
09 April 2014 09:37
With the arrival of Triumph's new 'LT' bagger version of its Thunderbird cruiser, never before have there been so many, truly credible alternatives to the all-dominant Harley-Davidson Road King. Moto Guzzi unveiled its hugely-impressive, all-new California 1400T in 2012, while the biggest threat of all to Harley, Indian, returned in the middle of last year with its own impressive offerings. ...
02 March 2012 14:34
The Triumph Explorer 1200 represents Triumph's first foray in to the ultra-competitive big adventure bike class, competing against bikes like the BMW R1200GS, KTM 990 Adventure and new Honda VFR1200X Crosstourer. Video: Triumph Explorer 1200 first ride MCN editor Marc Potter rode the Explorer 1200 recently at the world launch in Spain and was impressed: "I have to say I am ...
Marc Potter,
16 February 2012 12:11
Next week MCN is riding the new Triumph Explorer 1200 at its world launch in Spain, and we want to put your questions to the Triumph team who built the bike. The new 139bhp three-cylinder adventure bike uses an all-new engine, chassis and shaft-drive system which Triumph hopes will put it ahead of the best-selling BMW R1200GS and BMW R1200GS ...
Phil West,
26 December 2011 15:00
Triumph's imminent new 'R' version of its best-selling super naked is much more than a cosmetic update, MCN can now reveal – in fact it's been conceived to be "the ultimate Speed Triple"! So, where previous 'R' Triumphs, like the Street Triple and 675 Daytona, benefit mostly from uprated suspension and brakes, the new Speed Triple R goes significantly further ...
Guy Procter,
08 November 2011 13:09
Triumph's new Tiger Explorer will give its R1200GS rival a 135bhp mauling the firm has revealed. That makes the Explorer's all-new 1200cc+ motor 35% more powerful than its German rival's, at least for now, and on a par with the expected new Versys 1000. It also means it's the set to be the most powerful shaft-driven adventure tourer for the ...
Phil West,
24 October 2011 00:05
This special-edition version of the Bonneville, dubbed the 'Steve McQueen Edition' has just been unveiled by Triumph and will go on sale in 2012 – but only while stocks last. The bike has been designed to pay tribute to the late Hollywood star's love of Triumph motorcycles and will be limited to just 1100 examples. It's based on the current ...
Phil West,
21 October 2011 17:20
We've been browsing MCN bikes for sale every day to bring you some of our top picks from over 19,000 motorcycles for sale. Read more modern classics news and features Here's just a few of the fantastic modern classic machines available this week: '86 Honda CBX750, £795 Straight four came after Honda's switch to V4s - and won a TT.We say: Oddball ...
03 October 2011 14:31
Straight bars, 17in wheels, upside down forks, fox-eye headlights and an 800cc inline triple - could this be a secret, pre-production Triumph Street Triple 800? We wish. In fact this isn't a big-bore version of the 675cc Street Triple - one of the finest roadsters you can buy - but it could be the next best thing: a Triumph Tiger ...
15 September 2011 11:07
This supermoto version of Triumph's Tiger 800 is the latest special to be built by Jack Lilley Triumph of Ashford, Middlesex. It started life as a standard, new Tiger 800 to which Lilley has added a host of custom features. Wheels are 800 XC hubs laced to 17-inch Excel rims, it has a Speed Triple 1050 belly pan and front ...
Guy Procter,
28 March 2011 13:23
Triumph has bumped the spec of its 675cc Street Triples gently upward while keeping hold of the prices. The new bikes get a de-chromed look, chunkier aluminium bars (previously just on the Street Triple R model) and of course 'those' headlights. The standard bike will cost £6649 on the road with the R set at £7349. The makeover also marks ...
Save money by comparing quotes. It's quick and easy
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I have a couple of p mount carts, assume pmount is what would be used.
Also, what kind of inherent age related probs might i run into.
Free Shipping on All Orders 1-866-764-1801Vist our Online Store
I have a couple of p mount carts, assume pmount is what would be used.
Also, what kind of inherent age related probs might i run into.
humpty dumpty was pushed
I sold mine a few years ago. Hardly anyone works on them. They need a special guide to line the cart. Up. Parts are close to extinct. If you have someone local that knows how to get them up and running or has the tool or guage to get a cartridge to line up and can keep them going, cool.
thanks ern, i think i will steer clear
humpty dumpty was pushed
THought you were getting out of vinyl? Did the Rega sell? This table looks pretty cool but I know nothing about it and if ernie is right then upkeep may be impossible. Appears to be the same seller as the other stuff you PM'ed me about- nice collection for sale at good prices. Don't see a road trip a possible right now, but maybe in a couple weeks.
Going to go against the grain here and say grab it if you can. That table is considered by many to be the best linear tracking table ever made. I have seen them sell for ten times the asking price of this one (in perfect shape of course), so of this one works as it should I consider this one a steal.
HT Setup... Pioneer Elite SC-37, Polk Audio SDA-SRS 1.2TL's , Oppo BDP 93
Two Channel... Carver Statement 450~1 Vacuum Tube Monoblocks, Dodd Mid-line Tube Linestage with Psvane 12Ax7 tubes, Pioneer Pdd 9Mk II SACD Player, Yamaha PX-3 Turntable with Sumiko BPS EvoIII, Polk Audio SDA-SRS 1.2TLs.
"The problems we face today exist because the people who work for a living are outnumbered by those who vote for a living." Brad Shurett
The cool factor alone is worth a C note.
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Availability: Hôtel Siru
Comfort RoomMax Occupancy: 3 guests (up to 1 infant)
Standard Room (Privilege)Max Occupancy: 2 guests (up to 1 child, 1 infant)
Standard Single RoomMax Occupancy: 2 guests (up to 1 infant)
Hotel in Brussels (Brussels North - Rogier Square) near Belgian Comic Strip Center
This hotel is located in Brussels (Brussels North - Rogier Square), close to Rogier Square, Belgian Comic Strip Center, and Cathedral of St. Michael. Also nearby are La Grand Place and Manneken Pis Statue.
Hotel Features
Hôtel Siru provides a bar/lounge, complimentary wireless Internet access, a computer station, and a 24-hour front desk.
Room Amenities
Flat-screen televisions come with cable channels. Guestrooms also feature complimentary wireless Internet access, desks, and hair dryers.Information missing or incorrect? Tell us!
Hôtel Siru Amenities
- General
Hôtel Siru features a fitness center, an arcade/game room, and multilingual staff. A computer station is located on site and high-speed wireless Internet access is complimentary. A bar/lounge is on site where guests can unwind with a drink. For a surcharge, the property provides an area shuttle, a shopping center shuttle, and a train station pick-up service. A total renovation of this property was completed in 2011.
Hôtel Siru is a smoke-free property.
- Arcade/game room
- Bar/lounge
- Fitness facilities
- Number of buildings/towers - 1
- Computer station
- Elevator/lift
- 24-hour front desk
- Total number of rooms - 101
- Number of floors - 7
- Breakfast available (surcharge)
- Shopping center shuttle (surcharge)
- Free Wi-Fi
- Multilingual staff
- Area shuttle (surcharge)
- Train station pickup (surcharge)
- Smoke-free property
- Internet
Available in all rooms: Free Wi-Fi
Available in some public areas: Free Wi-Fi
- Room Amenities
- Connecting/adjoining rooms available
- Cribs/infant beds (complimentary)
- Private bathroom
- Phone
- Hair dryer
- Desk
- Iron/ironing board (on request)
- Cable TV service
- Flat-panel TV
- Free Wi-Fi
- Dining
Buffet breakfasts are available for a surcharge and are served each morning between 7 AM and 10:30 AM.
Hôtel Siru Policies & Fees
- Check-in
Check-in time starts at 3 PM
- Check-out
Check-out time is noon
- Payment types
Accepted at this hotel: American Express, MasterCard, Visa
- General
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
- No onsite parking is available. Special cancellation policies or charges may apply for group reservations (more than 8 rooms for the same hotel / stay dates).
No rollaway/extra beds available
- Fees
- Unless otherwise indicated, the charges below are not included in the overall room price. If not included in the overall room price, they will be charged by the property at check-in or check-out. Charges can vary, for example, based on length of stay or the room you book. This property told us they will charge you for the following:
We have included all charges provided to us by the property. However, charges can vary, for example, based on length of stay or the room you book.The following fees and deposits are charged by the property at time of service, check-in, or check-out.
- A tax is imposed by the city: EUR 5.25 per accommodation, per night
- Fitness facilitiesThe above list may not be comprehensive. Fees and deposits may not include tax and are subject to change.
- Breakfast fee: EUR 15 per person (approximately)
- Facilities fee: EUR 10 per person, per day and includes use of:
- Additional Details
This property offers transfers from the airport (surcharges may apply). Guests must contact the property with arrival details 24 hours prior to arrival, using the contact information on the booking confirmation. The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation. No pets and no service animals are allowed at this property.


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The Real Poop
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Career as Depicted in Popular or Unpopular Culture
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Bell Curve
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Botanists are always tracking plants, running experiments, writing scientific papers, and there's always the possibility, no, probability, that something may not work out. The rare fragrant plant that's supposed to bloom didn't. Too bad the poor botanist hiked 10 miles to bag the lichen.
Drat. That's a two-Excedrin day.
A botanist team has been working for two years to isolate a plant protein that might be the answer to world hunger. But, dang, compounds in flasks keep blowing up. Results are still inconclusive.
And did we mention the storm and stress of office politics?


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Golling Buick GMC, Lake orion, MI
We had my wife's torrent serviced there repeatedly. The heated seats did not work consistently for 2 years. While trying to fix the seats they removed the bezel around the shifter. Apparently, they broke the clips off of the back. They super glued it back on. It probably would not have been a big deal except that the glue was pouring out of the edges and very noticeable. So, we took it back so they could fix that and the heated seats (for the 5th time!) They still could not figure out what the issue was. There work was like a crappy oil change shop had decided to try to fix my car, everything looked sloppy and horrible. Finally, they hired a new assistant service manager. This guy got our seats fixed, replaced the counsel AND replaced the leather seat skin because they had damaged the seams by removing it and replacing it 8 times (not kidding about 8 times!). For about 2 years I could take the vehicle there and the service department was excellent! Everything fixed the first time. Everything clean and professional looking when the job was completed. Then, around March of 2012 we took the Torrent in for an issue that we had reported previously. The service manager was fired and a new manager hired. The awesome assistant manager was gone also. I assume that he left because they did not make him service manager. (he was running the place anyway). From there the service department went back to it's old ways. I called the dealer when her car had a hard start issue. I was 70 miles away and had 40 miles left in my bumper to bumper warranty. I told them that when I got there I would be over warranty by 30 miles. I could tow it in if they would like. They told me not to worry about 30 miles, it would be covered. When I got there The first thing that the service writer tried to do was sell me a battery for a car 3 years old! Then when I said to test it they said it would be $45. So, I declined. Then they tried to sell me an induction cleaning service. Then they told me that I was 30 miles over warranty so only power train was covered. (Um, I just called and spoke with you, you told me not to worry about the 30 miles) This was all before they even took the car back to check out my issue. They ended up putting a fuel pump in it. I went to pick it up and they apparently forgot to connect a plug. CEL light, no fuel gauge reading. This was right after starting it. They must have not test drove it after the install. It took them another full day to fix the issue. Then I find out that Golling GMC charged GM for a bad connector to cover their cost of dropping the fuel tank again. I went to start it when we picked it up and the same original issue occurred. I went back inside and spoke with the adviser. He stated that they could not duplicate the issue. Then why did they put a pump in?? I was told that any further diagnosis would be at a rate of $93 an hour. My neighbor took their Acadia in for a wheel bearing (under warranty), They changed they wheel bearing and when they neighbor picked it up the CEL was on. He went in and said that it was not on when he dropped it off. The advisor told him that it is probably unrelated issue and he would have to bring it back the next day to get it checked out. Why would a dealer that has a car in with a full bumper to bumper warranty let a vehicle leave with a CEL on? This is the worst run service department I have ever been to. Their sales department is terrible also. My neighbor who works for GM went in to buy a new Sierra (he already bought 2 from them and a Acadia) from Golling GMC He told the sales guy he wanted a sierra. The guy pointed at a Denali and said "there are the denalis) my neighbor told him that he did not want a denali, just a regular sierra. The sales guy pointed to the back of the lot, said "they are back there" and walked away! Needless to say he purchased at another dealer. Their parts department is pretty good. The guy that runs it is knowledgeable and very helpful. I have purchased from them a few times. I did have one issue. I needed some power steering line o-rings. They wanted $7 a piece, but insisted I but in packs of 10. I called a chevy dealer right down the street, they were the same price, but would sell them individually. I would NOT recommend going to Golling GMC. If you are in the area and stuck I would be willing to personally tow you somewhere else rather than subject you and your vehicle to their service department. You would be better of driving 20 miles to another GMC dealer.
Last edited by Pikey; 11-27-2012 at 09:11 AM.
1995 Silverado 4x4
6" BDS Suspension Lift-3" Body Lift-Add A Leaf in rear -Trailmaster SSV Shocks-Duel Steering Stabilizer Kit -AirAid Cold air intake-
4.56 Gears with Detroit Auburn Locker-Pro-Comp Traction Bars with duel shocks-Aluminum Skid Plate Kit-38.5" x 16.5" Mickey Thompson Baja Claws-Constant Dropping fuel gauge
2005 Yukon XL Jet Power Programmer, Bilstein Shocks, Bilstein rear springs, Helwig Anti-sway bars, EGR Window Visors, EGR Hood Shield, Denali Headlights, Headlight harness upgrade, GE NightHawk Bulbs, White Night Rear lighting system, Russell Braided SS brake lines, PowerStop Brake pads, PowerStop cross drilled and Slotted Rotors, http://www.gmtruckclub.com/forum/sho...5-GMC-Yukon-XL
2002 Silverado ext cab 2wd (Sold)
2003 Yukon XL (Totaled)
I'm sorry you had this experience. If you would like to file a dealership complaint, feel free to PM me with your information.
Evan, GM Customer Service
I filed a compliant when the heated seat/ center console issue happened. I also filed a complaint after the fuel pump issue. I have yet to hear anything from GM customer service about the complaint. This and the lack of interest in my complaint by GM customer service is what drove my family to sell the Torrent and purchase a Dodge
Again, I am sorry that you are dissatisfied with your situation. If there is anything I can do please feel free to contact me.
Evan, Chevrolet Customer Service
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After the December shootings at Sandy Hook Elementary School in Newtown, Conn., it's natural for parents to have questions about their children's safety at school.
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Thread: Americans spend 55 percent more on health care than Canadians. That's because Americans get more and better health care and don't have to wait for it
Americans spend 55 percent more on health care than Canadians. That's because Americans get more and better health care and don't have to wait for it
A single-payer system is probably the least effective way to achieve universal health insurance coverage.
Many people assume that Canadians enjoy universal healthcare coverage while receiving the same quality and quantity of medical goods and services as Americans. But the alleged superiority of single-payer health care is not consistent with the evidence. The reality is that, on average, Americans spend more of their income on healthcare than Canadians do but get faster access to more and better medical resources.
Healthcare appears to cost less in Canada than in the United States partly because Canadian government health insurance does not cover many advanced medical treatments and technologies that are commonly available to Americans. If Canadians had access to the same quality and quantity of healthcare resources that Americans enjoy, Canadas government health insurance monopoly would cost much more than it currently does.
Our recent study comparing healthcare in the United States and Canada shows that the public-private U.S. system outperforms the Canadian system on almost all the key indicators of available healthcare resources. The United States even performs nearly as well as Canada in terms of providing effective health insurance coverage for its population.
Americans spend 55 percent more than Canadians do on healthcare as a percentage of their national economy. But consider what Americans get for the money they spend: compared to Canada, the United States has 327 percent more MRI units and 183 percent more CT scanners per million population. The United States also produces 100 percent more inpatient surgical procedures per million population, and it has 14 percent more physicians and 19 percent more nurses per million population. U.S. hospitals are newer and better equipped than Canadian hospitals, and Americans have access to more new medicines than Canadians.
Other important facts about single-payer health insurance in Canada that are seldom reported by the pundits or politicians include the following:
In 1993, Canadian patients waited an average of 9.3 weeks between the time they saw their family physician and the time they actually received the treatment they needed. By 2007, the average wait time had almost doubled to 18.3 weeks. The median wait time in Canada is nearly double the wait time that physicians consider clinically reasonable.
The Canadian single-payer system does not cover prescription drugs on a universal basis. Only about one-third of the Canadian population is eligible for various government-financed drug programs. The remainder of the population has private sector drug insurance coverage or pays cash for outpatient drugs, just like in the United States.
Public drug plans in Canada often refuse to cover new drugs. On average, only 44 percent of all new drugs that were approved as safe and effective by the Canadian government in 2004 were actually covered by government drug insurance programs in October 2007. Even for the small percentage of new drugs that are covered by public drug programs, patients have to wait nearly one year, on average, after government approval for public insurance to start covering these new drugs.
Consumers in Canada and the United States spend roughly the same proportion of their per-capita GDP on prescription drugs (1.5 percent in Canada compared to 1.7 percent in the United States). As a percentage of per-capita, after-tax income, the cost burden of prescription drug spending is slightly higher in Canada (2.5 percent in Canada compared to 2.3 percent in the United States).
Between the fiscal years 1997-98 and 2006-07, government spending on healthcare grew in all 10 Canadian provinces at an average annual rate of 7.3 percent, while total available provincial revenue grew at an average annual rate of 5.9 percent and provincial GDP grew at an average annual rate of 5.6 percent. This level of government healthcare spending is unsustainable over the long haul.
Supporters of the Canadian healthcare system like to point out that the American system fails to provide universal health insurance coverage. This is true. But its also true that Canada doesnt do much better when it comes to actually delivering access to necessary medical care. Government data show that an estimated 1.7 million Canadiansin a country of around 33-34 millionwere unable to access a regular family physician in 2007. Without access to a family doctor, a person cant obtain regular primary care or referrals for elective specialty medical services.
Moreover, that 1.7 million estimate does not include the many Canadians who have access to a regular family doctor but are on waiting lists for specialist treatment. Access to a waiting list is not the same thing as access to healthcare. When Canadians cant get access to healthcare, they are no better off than uninsured Americans.
For that matter, how many Americans are really uninsured? We often hear that 47 million Americans lack health insurance. Yet research in the United States has demonstrated that the actual number of effectively uninsured Americans is less than half that number, and that being uninsured is usually only a temporary condition. Indeed, the estimated percentage of the population that was effectively uninsured for non-emergency, necessary medical services in 2007 was not that different in the United States and Canada: 7.9 percent in the United States compared to at least 6 percent in Canada.
Canadian patients who want to escape the delays in the public system are also barred from paying privately for healthcare services. In practical terms, Canadian patients are unable to buy quicker access or better care than the government health program provides. In this sense, Canadian patients on waiting lists are worse off than uninsured Americans, the latter of whom are at least legally allowed to use their own money or credit to buy healthcare.
Ironically, while Canadian-style healthcare appears to be gaining support in the United States, it is losing support and legitimacy in Canada. In a 2005 case challenging Quebecs government-run health insurance program, the Supreme Court of Canada declared the single-payer system a violation of a persons right to preserve his or her own health. Similar cases are now underway in two other Canadian provinces.
Ultimately, a single-payer system is probably the least effective way to achieve universal health insurance coverage. Canada has proved this in spades. The U.S. healthcare model may be flawed, but the Canadian model is far worse.
I agree with this theory. In Canada, we're seeing more and more private surgical clinics, equipped with the most advanced in medical technologies and techniques. The only caveat? You have to pay for it. However, these new clinics show no signs of diminishing as people who are sick of waiting for years on end for a simple corrective surgery can now go to one of these clinics and receive prompt and professional health care on par with, and exceeding, the public health available to all citizens.
It seems the only way to receive prompt health care is to be a criminal. Once you're in jail, the sick receive instant health care. Heaven forbid we violate their rights."The unforgivable crime is soft hitting. Do not hit at all if it can be avoided; but never hit softly."
"The pacifist is as surely a traitor to his country and to humanity as is the most brutal wrongdoer. "
Anyone can give up, it's the easiest thing in the world to do. But to hold it together when everyone else would understand if you fell apart, that's true strength.
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Powerful winds knocked out power for thousands in the Portland area on Saturday and forecasters say more stormy weather is in store.
The National Weather Service says winds were clocked as high as 70 mph on the coast and 40 mph in the Willamette Valley Saturday afternoon. There were isolated thunderstorms and lighting.
Forecasters say colder air moving in will dump 12 to 18 inches of snow in the Cascades overnight, with as much as two feet possible at higher elevations by the end of the weekend. However, strong winds forced Timberline Lodge to close ski lifts.
A warm front Sunday is expected to bring heavy rain to the Valley on Sunday, followed by dryer weather across Northwest Oregon and Southwest Washington by Monday night.
High winds knocked out power Saturday to tens of thousands of electricity customers in Western Washington, and crews worked to get them back on line for residents hoping to watch the Seattle Seahawks playoff game against the New Orleans Saints.
Puget Sound Energy reported more than 500 outages affecting nearly 40,000 customers Saturday morning, and said its crews were "in Beast Mode" working to restore it a reference to the bruising style of running back Marshawn Lynch.
CenturyLink Field, where the game was being played, was not affected by the outages. But some 33,000 customers in the area remained without power at game time, many of them on the Kitsap Peninsula west of Seattle.
By halftime the figure was back up to 40,000. On its Twitter feed, Puget Sound Energy urged businesses showing the game on TV to let customers know where they could watch it.
Seattle City Light said that at one point early Saturday, it had 28,000 customers without electricity, but it managed to restore 20,000 of them within a few hours. Nevertheless, more heavy rain, winds and hail swept through early Saturday afternoon, cutting power to thousands more.
The utilities advised people without power for the 1:30 p.m. PST game to find someplace else to watch it, such as the big screen at the Seattle Central Library.
Wind gusts in the region reached more than 50 mph, the National Weather Service reported. Crystal Mountain ski resort near Mount Rainier shut down its upper chair lifts.


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Billy Bargetzi, Instructor
Billy attended The University of Southern Mississippi, where he studied with Dr. Marta Hofacre, and was in just about every musical ensemble on campus with a trombone in it- including the award-winning USM trombone group The Hub Bones. Billy then studied Jazz Trombone with John Moak, Scott Whitfield, Paul Weikle, and Dr. Marc Dickman at The University of North Florida, and played lead and solo trombone in the award-winning UNF Jazz Ensemble I, along with several smaller groups. While at UNF, Billy also performed extensively at Disneyland, Walt Disney World and Universal Studios. Billy was also a clinician for the International Association of Jazz Educators (IAJE) in 1995.
As a teacher, Billy works with local schools and organizations doing masterclasses, and has a large studio of students- several of whom have placed very highly in the Alabama All-State Band and Orchestra system. Billy joined the UAH Faculty in 2012.
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You don't have to just grin and bear it! Go inside the bewitching opening night of the big, Broadway musical Matilda, starring Bertie Carvel, Lauren Ward, all those r-e-v-o-l-t-i-n-g children and more.
Ben Thompson takes us backstage at Holler If Ya Hear Me, where he tries on some wigs, almost has his dog stolen and talks to his co-stars...about himself.July 17, 2014
On this week's episode of The Broadway.com Show, Christopher Walken gets a deliciously evil new role, the Pump Boys and Dinettes crew reveals their diner do's and don'ts and the Great White Way goes to the dogs!July 16, 2014
In this week's peek at backstage life at If/Then, James Snyder meets a Twitter fan, plays a little guitar and participates in snack time shenanigans with LaChanze. Check it out!July 10, 2014
Watch video of Broadway's longest-running phenomenon!September 25, 2008
On a special episode of Looks Not Books, Queen Lesli answers your questions and gets answers from the cast of Matilda. Plus, a special commercial break from Miss Trunchbull. Don't miss it!May 27, 2014
If/Then's Jenn Colella shares what she loves about the new musical and explains why New York City is the center of the entire world.July 15, 2014
Sara Gettelfinger of Atomic discusses what it's like telling the story of the birth of the atomic bomb in this exciting new off-Broadway rock musical.July 16, 2014
Tamika Lawrence of If/Then gives us a taste of her classy rock show The Slay: A Rock Experience with Tamika Lawrence playing at 54 Below on July 21.July 15, 2014
It's the final episode of Les Miz star Ramin Karimloo's vlog. Don't be sad! This episode has tons of backstage shenanigans, interviews and puppets. We'll miss you, Ramin!June 10, 2014
Spotlight On: Mamma Mia!November 20, 2008
See a video sneak peek of Broadway's long-running sexy smash!September 25, 2008


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But the darkness she fled has caught up with her.
The high priests of Artemis once controlled the city, but a group of sorcerers are gaining power. And a strange group who call themselves followers of The Way further threaten the equilibrium. As Daria investigates Lucas's exploits into the darker side of the city, her life is endangered, and she takes refuge in the strange group of believers. She's drawn to Paul and his friends, even as she wrestles with their teachings.
When authorities imprison Lucas for a brutal crime, Daria wonders if even Paul's God can save him. Then she uncovers a shocking secret that could change everything-Lucas's fate, her position in his household, and the outcome of the tension between pagans and Christians. But only if she survives long enough to divulge what she knows.


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2012 Burgans Albarino
"Bright gold. Smoky peach and pear aromas are brightened by dusty minerals and lemon pith. Deep, expansive tangerine, nectarine and poached pear flavors are nicely lifted by white pepper and bitter quinine. Distinctly rich for the region but quite lively as well. Finishes with firm grip and lingering spiciness, leaving sweet citrus and pit fruit notes behind. According to the importer, yields were off by 40% in 2012, which likely accounts for the wine's uncommon weight and power."
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OK, there's no such thing as a political Darwin Award, but some ideas in politics are so stupid they deserve it.
Following up on my last post, I noticed this commentary by Lowman Henry of the Lincoln Institute outlining a Pollyanna view on Pennsylvania transportation. While I can sympathize with some of the author's points and his general worldview, I regretfully find this piece to be particularly unhelpful and frustrating, as it relies on misunderstandings, errors and myths to make the case for a mythical, unrealistic "third way" approach. Though I was initially looking forward to fisking this piece, my colleague Peter Samuel beat me to it--his take is well worth a read.
Instead, I'll focus on one particular meme found in Henry's piece that we're going to hear a lot more about in the weeks and months ahead--the false belief that PA could get a better deal by rebidding the Turnpike. Henry writes (Peter's comments are embedded in parenthesis in boldface):
Leasing the turnpike under a private/public partnership is, in and of itself, a good idea. Similar lease agreements in other states, and in many other nations, have proven highly beneficial as private operators, shorn of the shackles of over-unionization and other constraints of government, have been much more cost efficient. (AGREE)
But this proposed turnpike lease is a bad for two reasons: first, the revenue Pennsylvania would derive from the plan is far less than initially projected and far less than what the state could get if it were to rebid the contract.
(QUESTIONABLE: Who can know that you'd get more on a rebid? If anything financial conditions have deteriorated in the past couple of months - Lehman, Merrill etc. A better argument could probably be made that you'd get less on a rebid.)
Second, just like with the I-80 scheme, funds derived from the proposed turnpike lease would be diverted to other roads and bridges and not spent on maintenance and upgrading of the turnpike itself. Given the overwhelming capital needs of the turnpike system, this diversion of funds would be a huge financial blunder.
The $12.8 billion high bid by the Spanish consortium Abertis Infraestructuras to lease and operate the turnpike for 75 years falls far short of the lease's initial projected value which ranged between $15 billion and $20 billion depending upon who was making the estimation.
(MEANINGLESS: Henry is making too much of these those initial projections. They were little more than informed guesses by one analyst. Others said at the time he was much too high.)
The state received three bids after a process which initially yielded no takers. The bidders, apparently sensing a fire sale, came in low, with Abertis being the best of the bunch.
(WRONG: $12.8b was several billion higher than most people expected.)
Intentionally or not, Henry is playing in to the hands of Turnpike concession opponents who want to kick the can down the road and kill the lease with inaction. It seems they've latched on to this "we didn't get a good enough offer the first time, so let's rebid it" idea as their preferred strategy. Here's Senate Majority Leader Pileggi's staffer in the The Patriot-News yesterday, offering an early example of what will likely figure to be an oft-repeated talking point:
"The wiser course is to wait and address it next session," said Erik Arneson, the policy director for Senate Majority Leader Dominic Pileggi, R-Delaware County. "If that means we need to rebid [the lease deal], chances are pretty good we'll have more bidders next year."
This may score political points, but it's an irresponsible approach and demonstrates a woeful misunderstanding of the way the infrastructure market works. First, this would obviously kill the one viable option left on the table today--the Abertis/Citi Turnpike lease proposal--and leave an inefficient, patronage-ridden agency at the helm of a major state asset. If nothing changes in the status quo, politicians will get a free pass, the PA Turnpike Commission (PTC) will get more power, and taxpayers will be the real losers.
And the idea that the private sector would be eager to come back and bid more in a second round after watching Abertis & Citi get burned in the first is just plain ludicrous and disingenuous. Just look at Texas. Last year the state played banana republic, bending to political pressure to give the lucrative $3 billion Highway 121 toll project to a local public toll agency at the last minute, after having already run a competitive procurement process and nearly finalizing a contract with a private road investor-operator team for the same project. This followed immediately on the heels of a legislative moratorium on most privately-financed toll road projects. The message from state legislators and officials was, "Texas is Closed for Business."
And what's happened since then? While some concessionaires plan to stick it out until the Texas legislature can get its head on straight, others have already left the state, abandoning plans to do business (read: INVEST) there. They've deemed the political risk too high. It's entirely logical for a concessionaire to wonder "why should we invest millions into developing concession proposals when the state legislature seems bent on killing them?"
So let's put our thinking caps on: if you had just watched a PA Turnpike lease bid get pilloried and smothered by a bunch of legislators, would you be eager to jump right back in with an even higher bid the next year? If your answer is "probably not," you're just like most investors and operators who would avoid such masochism. I'm with Peter here--chances are you'd have fewer bidders and lower bids. Even those concessionaires willing to take a second bite at the apple would be nuts not to factor political risk into their bid. The math is simple: less competition + political risk = fewer & lower bids.
This is also relevant because the Turnpike is not the only public-private partnership (PPP) on the table. The PA legislature seems poised to pass new PPP-enabling legislation to facilitate future privately-financed road projects, and even the Turnpike-concession-hostile PTC is seriously looking to use PPPs for new construction projects on the Southern Beltway and the Mon Fayette Expressway. It's bad enough to shoot yourself in the foot when you have a $12.8B dollar project on the table. It's even worse if you jeopardize the prospects for future PPPs at the same time.
In the end, I'd like to think that our elected officials are competent enough to understand this simple logic--actions have consequences. The rules of gravity are not suspended because it's the Pennsylvania Turnpike. Private infrastructure financing will flow to those places that welcome it and facilitate it.
This isn't exactly rocket science. Hence, I have to think that those advocating such a naive and foolish idea as rebidding the Turnpike are either (1) woefully misinformed, or (2) cynical opportunists that will stoop to any depth to keep the patronage train at the PTC rolling along. Hold your nose Commonwealth taxpayers, because this rebidding idea stinks.


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Man charged in connection with Middlesbrough rapes
9:20pm Friday 7th December 2012 in News By Joanna Morris
POLICE have tonight charged a man in connection with two reported rapes in Middlesbrough.
In the first incident, a 19-year-old said she had been raped at an address near to Tollesby Road on Wednesday, August 8.
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JOPLIN, Mo. —
The inclination to blame God for human suffering or to deny his existence altogether has waned in the last century. As a journalist, I am inclined to credit my profession for this change in public attitude. Newspapers and television have made it abundantly clear that most human pain is self-imposed or is inflicted by one human being on another.
Consider that we have endured a century of total war, with worldwide suffering and atrocities on a scale not previously known. God was not to blame; people were.
Nor is God involved in random accidents that leave human victims, nor in the devastation caused when nature acts according to its laws. We may curse water when it floods and drowns, fire when it burns, volcanoes when they erupt and wind when it destroys property.
But intelligence equips humankind to confront natural forces, if not always to control them, then at least to moderate their destructive power.
Jesus himself warned about the fragility of houses built on sand, so we do best to insure ourselves against accidents and natural tragedies.
If many people continue to live in want, it is because the rest of us allow it. If a plane falls from the sky, it is because it was badly designed, maintained or flown.
I am impressed that people who suffer tragedy are disinclined to blame or deny God because of their misfortunes. Instead, we thank God that our losses weren't worse and ask his help in rebuilding our health, our homes and our lives.
Admittedly, physical and mental suffering is something else altogether. Chronic pain is senseless and debilitating. Death is the portal to eternal life; but when many Americans die, they are no longer the persons we knew in life. The health professions could do a better job of pain management, but why does God seemingly stand by when his own creatures decline?
The best answer I know is that God went through this before, when he denied his son's plea that he be spared torture and crucifixion. Human redemption was accomplished through God's own pain and death. That does not mean that all pain is redemptive, but it does suggest that our transformation into creatures worthy of happiness with our creator still involves suffering.
People often ask me what words a Christian can use to console a person in pain. My reply is that Christianity is a hopeful faith, but not an especially comforting one. What we can do is to imitate Christ in his generous acceptance of suffering.
Many people are inclined to treat even minor discomfort as pain, and to complain about any condition short of nirvana. The persistence of drug abuse demonstrates the human insistence on instant and carefree bliss.
In reality, some suffering appears to be an unavoidable part of the human condition. Fortunately, people of faith possess Jesus' own example of putting pain to good use.
David Yount is the author of 14 books on faith, spirituality and confident living. He answers readers at P.O. Box 2758, Woodbridge, VA 22153 and firstname.lastname@example.com.


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School of Medicine > Microbiology, Molecular Genetics and Immunology > COBRE > COBRE Pilot Projects
School of Medicine > Microbiology, Molecular Genetics and Immunology > COBRE > COBRE Pilot Projects
Revathi Govind, Ph.D., Kansas State University
Role of TcdR, the alternate sigma factor in Clostridium difficile virulence
Clostridium difficile is the leading cause of hospital-acquired diarrhea. Antibiotic use is the primary risk factor for the development of C. difficile-associated disease (CDAD) because it disrupts normal protective gut flora and enables C. difficile to colonize the colon. Toxigenic C. difficile strains produce two toxins, toxin A and toxin B that are considered to be the major virulence factors. The toxins encoding genes, tcdA and tcdB are part of a pathogenicity locus, which also carry the gene encodes for the toxin genes positive regulator tcdR. TcdR is an alternate sigma factor that binds with RNA polymerase core enzyme to make the holoenzyme that initiate transcription at tcdA and tcdB promoters. Alternate sigma factors are known to regulate virulence and virulence associated genes in many pathogenic bacteria. Including toxin genes, TcdR may regulate other virulence-associated genes in C. difficile. We have created and characterized, tcdR mutant in two different C. difficile strains. Mutation in tcdR affected both toxin production and sporulation in C. difficile. Microarray analysis revealed many differentially expressed sporulation-associated genes in tcdR mutant. In this project in our first aim, we propose to test the role of TcdR in C. difficile sporulation. In our second aim, we are proposing to monitor TcdR dependent promoter expression at cellular level, using a novel reporter system. During the current decade there has been a dramatic increase in the incidence and severity of C. difficile infections due to the emergence of hypertoxinogenic C. difficile strains. Our long- term goal is to unravel pathogenic mechanisms of C. difficile, thus new strategies to prevent, treat and manage C. difficile infection can be developed.
Jianming Qiu, Ph.D., University of Kansas Medical Center
Mechanisms of the cell cycle arrest induced during parvovirus B19 infection
Human parvovirus B19 (B19V) infection causes severe hematological disorders that in some cases can be fatal, including hydrops fetalis in pregnant women, transient aplastic crisis in patients with a high rate of red blood cell turnover, and chronic anemia in immunodeficient and immunocompromised patients. Currently, there are no specific antiviral drugs available to treat patients with B19V infection, and an effective vaccine to prevent B19V infection in high-risk individuals has yet to be developed. B19V replication is highly restricted to human erythroid progenitor cells (EPCs) in bone marrow and fetal liver. B19V-casued hematological disorders are largely due to direct killing of the EPCs, in which B19V replicates. B19V infection induces a DNA damage response (DDR) that is mainly mediated by activation of ATR. The B19V large non-structural protein NS1 is essential for B19V DNA replication and induces infected cells arrested at a phase with a 4N DNA content (4N phase). Replication of the B19V linear single-stranded (ss)DNA genome occurs in host cells arrested at the 4N phase, and is facilitated by the activation of ATR. Thus, B19V does not use the host double-stranded DNA replication machinery for replication of its ssDNA; rather, it appears to induce a DDR and subsequently to co-opt the host mechanism of DNA repair for its own replication. We hypothesize that during early infection, the ATR-mediated DDR induces intra-S phase arrest that facilitates viral DNA replication (repair) through inhibiting cellular DNA replication, and that in contrast, during late infection, the G2/M arrest induced by B19V NS1 promotes cell death.
We have established two experimental cell systems that will allow us to dissect the mechanism(s) of the cell cycle arrest during B19V infection: an efficient system of productive B19V infection involving the ex vivo-expansion of EPCs under conditions of hypoxia, which mimics the microenvironment of EPCs; a reverse genetics approach that involves transfection of a replicative form of B19V DNA into megakaryoblastoid UT7/Epo-S1 cells cultured under hypoxia. We will first explore a role of the ATR-Chk1 activation in inducing intra-S phase arrest during B19V infection and understand how the intra-S phase arrest facilitates B19V DNA replication. Second, we will characterize the B19V NS1-indcued cell cycle arrest at G2/M phase and understand the mechanism of how NS1 induces the G2/M arrest. Our studies will delineate the key molecular mechanisms of B19V replication and pathogenesis, which can be applied to develop anti-virus strategies for treating patients with B19V-casued hematological disorders.
Wolfram Zueckert, Ph.D., University of Kansas Medical Center
Mechanism of Borrelia surface lipoprotein secretion
Borrelia spirochetes, the causative agents of arthropod-borne Lyme borreliosis and relapsing fever, are often described as gram-negative bacteria due to their Gram stain properties and diderm, i.e. double-membrane envelopes. Yet, a closer examination reveals significant differences in cell envelope composition and architecture. Of particular importance for transmission and human disease is the unique Borrelia-vector/host interface, which is dominated by surface lipoproteins. Despite the emergence of these peripherally membrane-associated proteins as major virulence factors, targets of the immune response and premier vaccine candidates, the processing and targeting pathways that guide them to their sites of biological activity have been only partially defined. The overall objective of our research is to gain an understanding of spirochetal envelope biogenesis, with a focus on determining the secretion and sorting mechanisms of spirochetal lipoproteins. Our seminal studies using Borrelia burgdorferi as a model spirochete have shown that (i) surface lipoprotein localization determinants commonly localize to N-terminal tether peptides, (ii) translocation through the outer membrane (OM) requires an at least partially unfolded lipopeptide, (iii) accordingly, dimeric lipoproteins assemble into their final quarternary fold after reaching the bacterial surface, and (iv) translocation through the OM can be initiated by an unfolded C terminus. Preliminary studies also suggested that at least one of the predicted inner membrane Lol pathway orthologs is not directly involved in surface lipoprotein localization. We therefore hypothesize that surface localization requires maintenance of a translocation-competent intermediate, likely by interaction with a periplasmic holding chaperone, which may work in concert with a so far unidentified OM lipoprotein translocon. To test these hypotheses, we have formulated the following independent but synergistic specific aims:
1. To identify and define the periplasmic and OM pathway components governing Borrelia surface lipoprotein secretion by reverse and forward genetics approaches, using conditional knockouts of candidate periplasmic chaperones and a powerful combination of established FACS-based lipoprotein localization and novel suppressor screens.
2. To define the Borrelia OM lipoprotein translocation mechanism using in vivo site-specific photocrosslinking and pulse-chase experiments.
These studies will (i) achieve further milestones in our investigation of Borrelia lipoprotein secretion, (ii) shed more light on the evolution of bacterial protein export mechanisms, (iii) significantly increase our understanding of spirochetal virulence, and may (iv) translate into the design of future intervention strategies.
Bhaskar Das, Ph.D., University of Kansas Medical Center
Edward Stephens, Ph.D., University of Kansas Medical Center
Nanoparticle conjugated retinoids as effective therapeutic agents against HIV-infection
AIDS is still a pandemic that afflicts nearly 34 million people worldwide. Despite the development of highly active antiretroviral therapy (HAART) it has been not possible to eradicate AIDS because of lack of a vaccine and a lack of effective therapy against latently infected viruses. Furthermore, with the advent of HAART more individuals are living with AIDS, and the prevalence of cognitive impairment resulting from chronic CNS HIV exposure is increasing (Sacktor et al., 2001; Langford et al., 2003). Novel approaches are needed to develop drugs that may reach the latent reservoir of HIV-1 and that may penetrate the blood brain barrier to reduce the burden of chronic CNS HIV-1 replication. The long term goal of this application is to develop novel nanoparticle conjugated libraries to enhance nanoscience and nanotechnology research approaches that have the potential to make valuable contribution to biology and medicine and particularly giving emphasis to develop novel therapeutic agents against HIV-infection. We propose to develop nanoparticle conjugated libraries of drugs to utilize as therapeutic agents for HIV-infection, especially focusing on N-(4-hydroxyphenyl) retinamides (4-HPR) and its derivatives. 4-HPR or fenretinide is a synthetic derivative of retinoic acid or vitamin A (also called retinoid) and is an FDA approved drug under phase II clinical trials for many cancers. 4-HPR has been shown to be highly tolerable with minimal toxicities in humans. Our previous efforts to further modify 4-HPR led to the identification of an active moiety and allowed the synthesis of derivatives and peptidomimetic that retain the activity (Das et al and Das, Kalpana). 4-HPR was previously shown to be effective against HIV-1 replication (Blumenthal PNAS,2004,101(43), 15452-15457). Our preliminary studies have indicated that some of the derivatives of 4-HPR are more active in inhibiting the growth of HIV-1 than the parent 4-HPR. Based on these findings, we propose to first synthesize diversity oriented chemical libraries of 4-HPR. We will attach these functionalized 4-HPR molecules with ironoxide and trimethoxy silane based surface modified nanoparticles. We will evaluate the toxicity and efficacy of 4-HPR derivatives and nanoparticle libraries compounds using an in vitro cell culture system by using survival (MTS) assay. Starting with the lead compounds, we will iterate the process till we get a compound active at nanomolar level. Identification of new 4-HPR derivatives will lead to development of novel drugs against HIV-1. Our goal is to conduct a pilot study that can lead to a combined R01 application for future development of these compounds as therapeutic agents against HIV-1.
Susan Egan, Ph.D., University of Kansas
Inhibitors of AraC family virulence activators in Enterotoxigenic E. coli and Shigella
Many AraC-family transcriptional activators are required for the expression of virulence factors in bacteria that cause human disease. Loss of AraC-family activator function, by either genetic deletion or chemical inhibition, dramatically reduces disease in a large number of different pathogens. Among the pathogens that require AraC-family activators for disease are numerous examples that show rapidly increasing resistance to currently available antibiotics. The long-term goal of our work is to identify inhibitors of AraC-family proteins that have potential to be developed into novel antibacterial agents. The focus of the current proposal is the AraC-family activators Rns from Enterotoxigenic
E. coli [ETEC], and VirF from Shigella; both of which cause enormous worldwide morbidity and mortality and exhibit increasing resistance to antibiotics. Rns and VirF are required for the expression of virulence factors that are necessary for these two important human pathogens to cause disease. Therefore, our central hypothesis is that inhibition of transcription activation by Rns and VirF will prevent the expression of genes that encode critical virulence factors in ETEC and Shigella, and thereby reduce the ability of these pathogens to cause human disease. The objectives of this proposal will be met through two specific aims: Aim 1 will investigate a small molecule inhibitor, SE-1, that blocks the function of both Rns and VirF [as assayed in heterologous and in vitro systems]. We will test a set of chemical analogs of the inhibitor as a first step toward determining the structure activity relationship for the compound and optimizing the inhibitor. Our assays will include assay of the interactions of ETEC and Shigella with host epithelial cells in the presence of the inhibitors and use of the flow cytometry core facility. This chemical optimization could potentially be greatly enhanced through the use of structure-based design principles. Toward this end, Aim 2 will identify the binding site of SE-1 on one or more AraC family proteins, preferably through a high-resolution structure of the protein-inhibitor complex. Given that SE-1 is active against multiple AraC family activators, we propose the following alternative approaches: obtaining co-crystals of SE-1 with either ToxT [the master virulence regulator from Vibrio cholerae; has been successfully crystallized] or the RhaS DNA binding domain, or mutational analysis and inhibitor-binding assays. We expect to identify the binding site of SE-1 and to use this information in the design of more potent inhibitors of AraC family virulence regulators. The ultimate goal of this work is to identify inhibitors of AraC-family virulence regulators with the potential to be developed into antibacterial agents targeting pathogens that are responsible for massive worldwide illness and death.
This grant was made possible by NIH Grant Number P20 RR016443 from the COBRE program of the National Center for Research Resources and NIH Grant Number P30 GM103326 from the COBRE Program of the National Institute of General Medical Sciences.


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August 1, 2014
WASHINGTON (AP) - Treasury Secretary Jacob Lew says that he has now begun using all the extraordinary measures at his disposal to avoid hitting the debt ceiling.
In a letter to congressional leaders late Tuesday, Lew said that he started to use the final three bookkeeping tools available to provide borrowing room for paying the nation's bills. Lew said that there are no other "legal and prudent" options for extending the borrowing authority.
Lew said that his assessment of the date he will run out of maneuvering room had not changed from last week. Lew told Congress on Sept. 25 that the extraordinary measures would be exhausted no later than Oct. 17 and at that time the government would have about $30 billion in cash on hand.
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On January 8, 1993, seven people were murdered in the Brown's Chicken and Pasta restaurant that once stood at the corner of Northwest Highway and Smith Street. The restaurant was razed in 2001, and today, a Chase Bank occupies the corner.
The victims included, according to the Chicago Examiner, Brown's Chicken owners, husband and wife, 49-year old Lynn Ehlenfeldt and Richard Ehlenfeldt, 50, both of Arlington Heights, and Palatine residents and employees Guadalupe Maldonado, 46, Thomas Mennes, 32, and 31-year-old Marcus Nellsen.
Michael Castro,16, and 17-year-old Rico Solis, both of Palatine who attended Palatine High School, also were among those killed.
Juan Luna and James Degorski, former Fremd High School students, were arrested in 2002 for committing the murders. A former girlfriend of Degorski came forward and told authorities both men had confessed to being responsible for the crimes, the Chicago Tribue reported.
The murders occurred just after 9 p.m., the Daily Herald reported, 20 years ago today, on January 8, 1993. The bodies weren't discovered until early on January 9. All of victims were shot to death and left in the restaurant's freezer.
Luna was sentenced to life in prison without parole in May of 2007, the Chicago Tribune reported, and the Huffington Post reported that Degorski also received a life sentence in 2009. Both men were charged with seven counts of murder.
Palatine Police came under an intense amount of scrutinty, according to former Palatine Village Councilman Jack Wagner, who was on the village council at the time of the murders and during the subsequent investigation to find the killers.
"If one good thing came out of it, it was that the Palatine Police were vindicated, they were being pressured to charge the wrong people by certain parts of the media, and they refused to take part in that," Wagner said.
The motive for the murders was determined to be robbery. Luna and Degorski got way with less than $2,000.


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THE WISTOW MAZEToday
THE WISTOW MAZE - Winner of Leicestershire's "Best Visitor Attraction"
Kettering by the Sea 2014.Today
The popular Kettering by the Sea comes to The Market Place again this year.
New FSB breakfast club at Silverstone Golf ClubToday
Network with local businesses and build your contacts at our FSB Breakfast Club on the first Friday of each month.
Great British SummerToday
Celebrate the Great British Summer at Kids Play
Steak Night at The Stags Head.Today
Steak Night at The Stags Head. Steak for 2 offer.
Car Boot & Farmers' Market26/07/2014
Holcot Car Boot. We are not an early morning car boot, we are on all day Saturday.
Wicksteed Park Car Boot Sales.27/07/2014
The Wicksteed Park Car Boot Sales will take place every Sunday and is dependent on the weather.
Muppets Most Wanted28/07/2014
Lighthouse Movies. Two Frogs! One Pig! Intrigue! Music! Mayhem! Muppets!
The Book Thief28/07/2014
Based on the beloved international best selling book.
Cransley Hospice Coach Trip to Melton Mowbray29/07/2014
Coach trip to Melton Mowbray Market and Gates Garden Centre


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TACOMA, Wash. -- For Thu Ament, Foss High School is his second home.
"We call it the Foss Family," he says. Ament has been a principal at the school for four years.
"I came here with the intent to try to give back."
His roots run deep. He and his brother were also once students.
"Walking through here brings back a lot of memories."
So it was with a heavy heart that he stood in front of the entire student body last week and broke the news.
"I didn't have to say a whole lot to get a whole gym of kids to be quiet because they were silent, and that told me a lot."
Foss may have to close its doors next year. It has the lowest high school enrollment in Tacoma, with just over 1,100 students. The district says closing Foss would save $2 million next year, with the hope that, in three years, it can open again.
Cynthia Lenihan coordinates the International Baccalaureate Program. It's intensive college prep. The district says it will try to keep the program intact by moving it to another school, but Lenihan wonders if that's possible.
"If we have to disburse between four different high schools, I don't know. It would be really be difficult to do that," she says.
The district says another option would be closing five elementary schools with an enrollment under 300. It would also look into draining its $30 million reserve fund down to $10 million.
"When we're faced with a $25 million shortfall over the next three years, yes, we're having to make some tough decisions," says Tacoma district spokesman Dan Voepel.
Principal Ament hopes there are other options. He says there is really no other place like Foss.
"I believe if we have a lot of collective minds, we can be creative to make the cuts where we can not impact kids and the community," he says.
The school district will hold a series of five meetings to discuss options on the table. All meetings will be from 6:00 p.m. to 7:30 p.m.:
Tues., Jan. 25th at First Creek Middle School
Wed, January 26th at Giaudrone Middle School
Tues., February 1st at Jason Lee Middle School
Wed., February 2nd at Truman Middle School
Thurs., February 3rd at Meeker Middle School
Voelpel said the meetings are about the district's budget crisis and how it's affecting the entire school district, not just Foss High School. They are also considering closing four elementary schools as well as laying off about 70 other positions around the district.


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By Corbin Hosler
The Norman Transcript
NORMAN — Entering the season with a young squad, Norman North coach Dennis Etter knew it would take some time for his team to reach the top of its game and move up the standings.
He just thought it would take a little more time than it did.
The Timberwolves shot a team-best 300 to win the Timberwolves Invitational Tuesday at the Jimmie Austin OU Golf Club, finishing three strokes ahead of second-place Edmond North to win the event.
"We've been in the middle of the pack at every other tournament we've played in this year," Etter said. "Don't get me wrong, I knew we could play this well, but it's still a bit of a surprise."
All four of North's scorers shot below 80 to hand the T-Wolves, which start three sophomores and a junior, their first tournament victory of the season. The win came against some of the top teams in the state, including Edmond North, Jenks and Owasso.
"Every one of them just had a really good day," Etter said. "That's a big improvement over how we've shot before."
Thomas Ludwig carded a 73 to lead the T-Wolves after what was originally planned to be a 36-hole tournament was shortened to 18 due to weather conditions.
"I felt pretty good out there today," he said. "The greens rolled pretty well today and I stayed out of the bunkers. This is the first tournament where we've all really played well as a team. How we shot today, that was a pretty big confidence booster for us."
Thomas Johnson shot a 74, and Ryan Trousdale and Connor Mears rounded out the top four with a 79 and a 74, respectively. The T-Wolves won by three strokes and finished four shots ahead of Edmond Santa Fe, six ahead of Tulsa Union and seven ahead of Jenks, all teams expected to contend for a state title.
Norman High, which like North is moving through the year with a young core, shot a 351 with Turner Howe leading the way with a 76.
Coach Bruce Renfroe said that, while there have been some ups and downs this season, there has already been some progress made in the first few weeks of the year.
"With a roster this young, you're building for that future," he said. "We didn't shoot as well as I would have liked today, but every tournament is more experience for these guys to learn from."
For local news and more, subscribe to The Norman Transcript Smart Edition, or our print edition.


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About the TRANSforming Gender SymposiumThe University of Colorado-Boulder's TRANSforming Gender Symposium is celebrating its seventh year! The Symposium hosts national and local transgender and genderqueer activists to address issues surrounding transgender, genderqueer and related identities. Previous presenters and participants have included academics, health professionals, community organizers, performance and visual artists, students, faculty, staff, youth, family members, community members, and other interested folks.
The Symposium is free and open to the public! (We will be asking for a cost-free registration at a later date.) In addition to nationally recognized speakers, we are excited to be including workshops and panels throughout the day.
We are calling for workshop, paper, and caucus proposals from campus, local and national folks interested in presenting at the Symposium. Past symposia have covered some of the following topics as they relate to trans identities: spirituality, health care, transfeminism/transmisogyny, sexuality, poetry, media, racism and classism, mental health, sports and athletics, and much more. Please see below for more information.
Workshop and Paper Guidelines
TopicsWorkshop topics may encompass many aspects of gender identity, gender expression, trans identities, transitioning, and other transgender and intersex related issues. Panels, papers, and workshops will address these issues through a variety of lenses as well as in relation to specific contexts such as social justice, academia or health. We particularly encourage proposals that are relevant to trans identities such as intersex, two-spirit, genderqueer, and people of color.
PapersTo submit a paper proposal, please submit a 250 word abstract via the Paper Submission Form.
CaucusesTo submit a Caucus proposal, please submit a brief description of the caucus: who the facilitator will be, for whom it is intended, and whether it is a closed session.
Target AudienceThis conference is open to all age groups including local high school students and community members. Please try to formulate your workshops so that they are appropriate for all ages. Please inform us of any topics or discussions you feel may be difficult for a certain age group to participate in, and we will work with you to formulate a statement about age-appropriate audiences.
Session LengthWorkshops and panels will be 1 hour and 15 minutes. If you would like a double session please explain why and provide a detailed schedule.
Tentative Schedule (subject to change):The Symposium will have an afternoon session on Friday, March 14, 2014, and three sessions on Saturday, March 15, 2014.
DEADLINE FOR SUBMISSIONS: January 31ST, 2014Please complete the information below and submit to Scarlet Bowen at firstname.lastname@example.com. For more information about the Symposium, including travel and hotel recommendations, contact (303) 492-1377 or email@example.com.


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Animal Kingdom and Shackleford square off in New York. A preview of Saturday's Belmont Stakes, the final leg of racing's Triple Crown.
A preview of Saturday's Grade 1 Preakness Stakes featuring Kentucky Derby winner Animal Kingdom.
Animal Kingdom looks to win the second leg of the Triple Crown at the 2011 Preakness Stake on Saturday. But can he become the first Triple Crown winner in 32 years?
An in-depth preview of the field for this year's Kentucky Derby, including a horse-by-horse look at all the contenders, along with wagering selections.
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