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Non-hazardous substitute for ethidium bromide?
Posted 15 August 2012 - 02:14 PM
I am interested in hearing what others are using.
Our lab is required to treat used EtBr (in gels and running buffer) as hazardous waste. This is expensive ($100 per pickup, 25 L pail of used gels), time consuming, paperwork, hastle, etc. To remove the EtBr from the used running buffer we stir overnight with "tea bags" from Amresco. Afterward we pour the running buffer down the sink. After 50 uses we discard the used tea bags with our used gel waste.
I've tested EZ Vision (Amresco), Sybrsafe (Invitrogen) and GelRed (Biotium).
I'm not plugging any product here. I only care about what works without any bias as to where I buy it from. We only have the camera filter for EtBr. EZ Vision and Sybrsafe require a the Sybrgreen camera filter ($900 - yikes!). However, GelRed works well with the EtBr camera filter.
Although Sybersafe and EZ-Vision appear to work well, the long exposure required (EtBr camera filter) causes too much background fluorescence. I would like to test the EZ-Vision with a Sybersafe camera filter because the safety data on this product appears to be very thorough. I think EZ-Vision can actually be discarded as a truly non-hazardous substance in regular garbage (double bag the used gels and pour used running buffer down sink with running tap water).
For time being I opted to pursue GelRed further because it uses EtBr filter.
At first I tried using GelRed in the gel but the PCR bands were too strong with smeary frown. I suspect the problem of heavy smeary frown may be due to overloading the gel. However, the smeary frown was still a problem, even after I tested serial dilutions of the DNA and different concentrations of GelRed in the gel.
In this whole experiment I tested a variety of PCRs between 100 bp and 1,000 bp.
I decided to try instead using GelRed in the loading buffer. I used gels and running buffer without any dye. This appears to work much better.
I tried the following amounts of GelRed in loading buffer:
1ul/100ul, 1ul/200ul, 1ul/500ul, 1ul/1ml, 1ul/2ml, 1ul/5ml
I added 5ul of GelRed loading buffers to 25 ul PCRs and loaded 10 ul into gel.
The gel and running buffer (1x TAE) do not contain any dye.
1/100 and 1/200 GelRed in loading buffer gives too strong staining for most of our PCRs, which requires us to titrate how much PCR to load on gel. It also gives some background fluorescence.
1/500, 1/1,000 and 1/2,000 GelRed in loading buffer appear to give optimal staining for most of our PCRs, which does not require us to titrate how much PCR to load on gel.
Although 1/5,000 GelRed in loading buffer works, it may give too weak staining for some of our PCRs.
See photo.
Several years ago we stopped using agarose gels and instead use agarose-composite polysaccharide gels. These gels are stronger, more flexible, more clear (less background fluorescence) and have wider separation range than agarose. At first we used Visigel from Stratagene, but they discontinued the product in 2007. They kindly gave me the recipe. If anyone is interested I will post my recipes. It consists of 0.7% agarose and 1% clarified high molecular polysaccharide from Locust Bean Gum (aka, carobe bean). As far as I'm aware the only folk that make cHMW-LGB is Diversified Biotech, (Agarmate, AGM-200).
If anyone is interested I will post my recipes.
Posted 15 August 2012 - 03:01 PM
Posted 15 August 2012 - 04:44 PM
- ascacioc likes this
Posted 15 August 2012 - 05:47 PM
I add gelred to my agarose at a dilution of about 1/40 000, although they recommend 1/10 000.
Posted 15 August 2012 - 10:57 PM
Posted 16 August 2012 - 02:49 AM
Posted 16 August 2012 - 08:51 AM
Anyway the other chemicals usually involved (formaldehyde, acids) and the silver itself (silver nitrate) not everyone might like. And the dying protocol is more laborious.
Here are some other alternatives (somewhere posted this pdf earlier in an other thread, too): DNA14.pdf 73.04KB 649 downloads
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that did belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 16 August 2012 - 10:47 AM
RE: "phage434", I will post my recipe for composite agarose-cHMWLGB gels in my next reply.
RE: "bob1", I agree. All DNA binding agent must be handled safely. Same applies for anything that stains cells (precautionary principle). Wear gloves and change them regularly. I am agnostic regarding any product - as long as it works. I like the literature for EZ-Vision DNA stain (Amresco). It appears to be the closest thing to being non-hazardous. The US gov't toxicology program seems to support this.
RE: "leelee", "Curtis", "ascacioc" and "hobglobin", I work in a government diagnostic lab that uses PCR to routinely test for infectious diseases of livestock animals. So post run staining is not an option that will work for us because we have to produce results in a timely manner. That's why I'm looking for a non-hazardous alternatives to EtBr. One which works at least as well as EtBr and will be more efficient for our lab staff.
How terrible is RedSafe? Also, really how safe is it? That's a problem I have with chemical companies. So many new chemicals are developed and produced every day, yet apparently with minimal safety standards. Oh well.
Back to GelRed. So far I've tried several concentrations of [GelRed] in the gels, as follows:
- 1/10,000 (as recommended by manufacturer)
- 1/20,000
- 1/40,000
- 1/80,000
All resulted in smeary frown bands (remind me and I can post example photos). The problem is not in our gels itself because the 100 bp ladder looks fine with all [GelRed], albeit fainter at lower [GelRed]. However, GelRed does work very well when used only in the loading buffer and not in the gel.
Posted 16 August 2012 - 11:42 AM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that did belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 18 August 2012 - 06:43 AM
Modified from Perlman et al. 1987. Improved Resolution of DNA Fragments in Polysaccharide-Supplemented Agarose Gels. Analytical Biochemistry. 163: 247-254.
Agarmate-Agarose slurry: Updated May 25, 2012
Supplies for 250 mL of slurry:
- clean, dry 500 mL wide mouth plastic screw cap bottle (ex/ empty ultrapure diH2O bottle)
- 250 mL Absolute 100% ethanol
- 30 grams Agarmate, Diversified Biotech cat# AGM-200 (200 gm, High Molecular Weight Clarified Locust Bean Gum [HMW CLBG])
- 25 grams Agarose, Bio-Rad cat# 161-3101 (125 gm, Molecular Biology Agarose)
1. Add the 250 mL ethanol, then 30 grams Agarmate and 25 grams Agarose into the 500 mL bottle.
2. Close the lid, then shake the bottle for 10 sec. Label the date and contents. Store at room temp for 1 year.
Pouring a gel:
- clean, dry 1 L Erlenmeyer flask; Tuttle flask cover (Fisher, VWR, etc)
- clean 15 cm x 25 cm gel tray, top edges are marked for even spacing of combs and marked with red dots at "top end" and red dots at "bottom end"
- gel casting stand; 7 plastic 20 well gel combs (glued to homemade 1 cm x 1 cm x 20 cm acrylic plastic supports)
- 20 mL Agarmate-Agarose slurry
- 350 mL 1x Tris-Acetate EDTA (TAE) buffer, pH 8.3 (without ethidium bromide [EtBr])
- 25 µL of 0.2 µg/µL EtBr (optional, can pour gels without any dye and use instead DNA dye in loading buffer)
- gel box containing just enough 1x TAE (with EtBr or without if using dye in loading buffer) to barely submerge the raised central platform
- o-ring with support stand
- microwave oven set to high; silicone oven mitts; safety goggles
- if using loading buffer containing DNA staining dye, we recommend using GelRed (Biotium) at 1 in 2,000 in 6x loading buffer
- 6x loading buffer (1x TAE, 50% w/v sucrose, 0.1% bromophenol blue, 0.1% xylene cyanole), store in 1.5 mL tubes at - 20*C
1. Centre the gel tray in the casting stand, then lock the assembly.
2. Level the gel tray assembly in a fume hood. Stand the o-ring at the back left corner of the gel tray.
3. Vigorously shake the bottle of Agarmate-Agarose slurry. (Until thoroughly mixed/resuspended.)
4. In a polypropylene 25 mL cylinder immediately measure 20 mL of the slurry, then pour it into the Erlenmeyer flask.
5. Add 330 mL of 1x TAE to the flask, swirl for 10 sec. Rinse the cylinder with the remaining 20 mL TAE and pour this into the flask.
6. Place the Tuttle flask cover on the flask and microwave 3 min.
7. Carefully remove the flask from the microwave, lightly weigh down the Tuttle cover, swirl gently until no more steam.
8. Swirl the flask vigorously for 10 sec.
9. Microwave for 30 sec.
10. Repeat steps 7 -9 two more times, except microwave 15 sec or until Tuttle cover can be heard jingling.
11. Repeat steps 7 and 8. Immediately transfer the flask into the fume hood while the molten gel is still hot.
12. Remove the Tuttle cover. Only if using EtBr in the gel: immediately pipet 25 µL of EtBr onto the centre of the molten gel. Otherwise do not add any dye.
13. (This step only if using EtBr in the gel. Replace the Tuttle cover. Swirl the flask vigorously for 20 sec to evenly distribute the EtBr in the molten gel.)
14. Pour the molten gel into the gel tray. Put the flask in the o-ring to drain the remaining molten gel.
15. Immediately insert seven gel combs. Center them, evenly spaced and in parallel.
16. Let the gel solidify for 1 hour. (If > 1 hour the gel will need longer to rehydrate before removing the combs.)
17. Unlock the gel assembly. Remove the gel tray with the combs still intact.
18. Place gel tray with combs in the gel box (red|red, black|black). Adjust volume of 1x TAE (with EtBr) to barely submerge the gel.
19. Let sit in the TAE for 1 minute to allow the wells to rehydrate. (Add 1 minute for every extra hour after pouring.)
20. Carefully tap/tip each comb. (This allows TAE to enter the wells and lubricate the combs.)
21. Gently remove each comb. Tug|wiggle alternating left|right up no more than 2 mm at a time.
22. Replace the gel box lid. Cover the gel box under a shopping basket (to block out light and prevent photo bleaching).
23. Store the gel box with gels and 1x TAE in the walk-in cold room. (This prevents growth of yeast in the TAE).
24. Wash the entire flask with hot tap water and Sparkleen detergent (Fisher). Rinse several times with hot tap water.
25. Rinse and rub the combs under running warm tap water. (Occasionally clean with warm tap water and Sparkleen).
Store gels and 1x TAE at 4*C when not in use. Example, on a cart in walkin fridge. If using EtBr in gel and in running buffer, store in dark.
Example, we cover gel box on cart with one of those heavy plastic shopping baskets which can be purchased at large supermarkets.
Posted 11 September 2012 - 06:30 AM
I needed to ask something about this product so if anyone has any experience in using this then please let me know.
Moved and merged so as to keep recent discussion in one place. Bob
- Mad Researcher likes this
Mad Researcher
Posted 11 September 2012 - 08:56 PM
Posted 12 September 2012 - 02:51 AM
i use the VWR 6X loading dye.
But when i run the gel i lose the loading dye. Is it caused due to the gel red?
I did it with SYBR and it worked fine there.
Mad Researcher
Posted 12 September 2012 - 06:27 AM
- leelee and Mad Researcher like this
talent does what it can
genius does what it must
i used to do what i got paid to do
Posted 12 September 2012 - 06:30 AM
As to why loading dye is lost when using GelRed in TBE?
DO you mean that the dyes in the loading buffer disappear during electrophoresis?
What dyes are in your DNA loading buffer? xylene cyanole and bromophenol blue?
Have you checked the pH of your running buffer before and after use?
It can change after use. And of course if it is not slightly alkaline (pH 8) to begin with,
the dyes can change to lighter colors.
See my earlier posts above. We now no longer use ethidium bromide.
Also, we pour our gels without any dye in them and without any dye in running buffer (see recipe in my earlier post above).
We also no longer use GelRed as it sometimes creates migration artifacts for some of our PCR assays and sometimes causes
smearing. GelRed is also classified as hazardous waste, so is less convenient overall. GelRed is VERY sensitive, giving very
bright fluorescence (UV 302 nm transilluminator). So, it looked promising at first, but now we use EZ-Vision instead.
We extensively tested using GelRed diluted 1 in 2,000 in our 6x DNA loading buffer, adding 5 uL to our 25 uL PCR.
Then loading 10 uL into gel. But the used gels now end up with GelRed in them so have to be discarded the
same as EtBr haz waste. Which is something we want to avoid (we are a diagnostic lab, short staff and pressed for time).
Also, GelRed causes some amplicons to migrate at different rate than expected. Regardless if used in gel or in DNA loading buffer.
Again, not good for a diagnostic lab. So we stopped using GelRed.
Finally we discovered that EZ-Vision (Amresco) performs same as EtBr. That is, no migration artifacts, no affect on migration rate
for any of our PCR assays and same band intensity and detection limit as EtBr. One difference to note is that EZ-Vision needs to use
UV transilluminator set to 365 nm (whereas EtBr uses UV at 302 nm).
We use EZ-Vision concentrate (supplied as 5 mL dropper bottle) diluted 1 in 100 in our 6x DNA loading buffer.
We add 5 uL of DNA loading buffer to our 25 uL PCR, mix, load 10 uL per well. Bands migrate precisely as expected (we ran many
different PCR assays, those whose migration were affected by GelRed migrated as expected when using EZ-Vision in DNA loading buffer.
We consider EZ-Vision as non-hazardous (because Amresco supplies plenty of documentation and studies
backing up their claim). So we can discard used running buffer down sink with running tap water and we can dispose used gels
(double bagged for smell, due to TAE which airborne yeast will ferment) in our regular waste.
For our understaffed diagnostic lab that counts every penny we spend, this is a big deal. Our staff time is valuable.
The hazardous waste disposal costs us $100 per 25 L pail of used gels and EtBr "tea bags".
I will post a few photos and more detailed recipe.
Also tagged with one or more of these keywords: non-hazardous, DNA stain, PCR
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
PCR/Thermocycler Use Case Questions
Started by teilhardo, 30 Jan 2019 pcr, thermocycler, thermocycling
Student Forums (for study, homework and non technical questions) →
-Molecular Biology- →
PCR/Thermocycler Use Case Questions
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Started by Saadlee, 01 Aug 2018 PCR, cloning
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Started by Delimar Rodriguez, 27 Apr 2018 molecular, cloning, digest, pcr and 1 more...


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Report: Expanding gambling could make Florida 'less attractive' tourist destination
If Florida decides to expand casino gambling, the move could harm the state and Orlando's tourist brand, according to a new gaming report commissioned by the Florida Legislature.
The broad-ranging report, released late Monday by the Spectrum Gaming Group, found that Florida has one of the nation's most competitive gambling markets with a parimutuel industry that "resembles a circular firing squad" and a regulatory environment that is a "mess." It concluded that any expansion of gambling — such as destination resorts — will result in more expansion because "the industry rarely shrinks."
"The brand equity of Orlando has benefits for the entire state" and "expanded gambling may fundamentally change the state of Florida as a place to live and visit," the report said. "Rather than benefitting the state, expanded gambling (especially casinos) could make Florida a less-attractive tourist destination."
The 320-page report by the New Jersey-based gambling consulting firm will serve as a foundation for the legislature's attempt to rewrite the state's gambling laws next session. Lawmakers will also decide whether or not to allow the expansion of casinos, such as those sought by Genting and Las Vegas Sands in Miami, and whether to renew the gaming compact with the Seminole Tribe.
Legislators paid Spectrum $388,000 to assess Florida's gambling market and this is the first of two parts. The second report, on the economic impact of gaming on communities, is due in October.
But the head of the Senate Gaming Committee emphasized that the goal of the report is not to make recommendations but to provide lawmakers with a road map of the terrain. Lawmakers plan a series of meetings around the state, beginning in the fall, to discuss the issues.
"The report does not, and will not make policy recommendations,'' said Sen. Garrett Richter, R-Naples, in a statement. He said it will be the responsibility of the committee "to review gambling statutes, to address the ambiguities, inconsistencies, and exceptions in current law, and to craft an action plan."
Crafting a state gambling plan has been an elusive goal for decades. As the state's pari-mutuel industry has rapidly declined in popularity, the industry has pushed to offset its losses by installing card rooms and slot machines — a situation that the report notes has helped prop up and subsidize the declining greyhound, jai alai and thoroughbred racing industries. During that time, technology has advances to make it possible for games that mimic slot machines to be operated on video terminals and for casino games, such as roulette and craps. to be operated on slot machine platforms.
The result has been a patchwork of regulations and major gaps in the law. If Gov. Rick Scott and the legislature remain silent on revising the statutes, "gaming will evolve in Florida whether or not the Florida Legislature develops a plan and puts that plan into action,'' the report concluded. "Absent any plan, however, that evolution would be haphazard and would be far less likely to address or advance any public-policy goals."
Among the report's findings:
• Casinos provide competition but not necessarily cannibalization of existing businesses. "The evidence suggests that casinos are likely to have a negative impact on lotteries, and some other businesses may see decreased revenue as a result of a casino's opening. But this is no different from what happens when any other business opens. Certainly some of the revenues for a new casino would come at the expense of other, existing gambling firms in Florida."
• Gambling expansion is headed to the Panhandle. The Poarch Band of Creek Indians, based in Atmore, AL, has land in Escambia County and now operates the Gretna racetrack which is operating controversial flag drop races. The tribe "has options to own, or agreements to control 10 pari-mutuel permits along the Interstate 10 corridor between Pensacola and Jacksonville.
• State tax revenue from parimutuel gambling has plummeted 99 percent for jai alai and greyhound racing since their hey day of 1985 and dropped 39 percent for horse racing.
• Florida regulators fail to collect accurate data. The report found that the Division of Parimutuel Wagering made no effort to independently verify information supplied by the tracks and the Isle of Capri Pompani harness track "grossly overstated" its purse numbers from 2009-2012 and Gulfstream Racetrack understate simulcast revenue by $605 million.
• Florida's largest greyhound tracks are ready to give up their dog racing. With losses of $35 million from greyhound racing in the last year, only three tracks made a profit and that was because of revenues from cardrooms. Michael Glenn, general manager of the said his company would shut down the dog track if it could. "There just are not enough folks out there to come to the track and wager on these races,'' he told Spectrum. "There is not any interest."
• Expansion begets more expansion. "The industry rarely shrinks, and quite often, expands as a result of expansion."
• Adding table games to the menu at racinos would increase revenues for slot machines, not reduce it. "The addition of house-banked table games such as blackjack to a slots-only casino can serve to increase slot revenue. This seems counter-intuitive (the natural assumption is that new table games would simply cannibalize existing slot play) but experience in markets that have added tables to casinos that previously offered only slot machines shows otherwise."
• Destination casinos that offer mix of resort and convention space are not panaceas "nor do we ignore the downside or the challenges they present to local communities. By definition, they increase traffic and create other demands on public services. The more successful they are in generating revenue, the more such demands increase."
• Olando should stay away from casinos. "In our view, Orlando's ability to grow its conventions and meetings business in the face of this national trend underscores an important asset in this market: Orlando's strength in attracting business travelers is growing without gaming, and that absence is to some degree fueling that growth. Orlando has carved out a significant, profitable niche in that national market, and gaming would clearly be antithetical to that image and its ability to dominate that important segment."


text:
International Family Therapy Association
Benefit to MembersAs a member of IFTA you will receive a subscription to the Journal of Family Psychotherapy. This is the official journal of IFTA and is sent to you as part of your membership fee and his available online as well.
The online International Connection newsletter provides you with updates about the organization and additional articles written by members worldwide.
A new Members Only Research E-News Forum is sent quarterly highlighting research in marriage and family therapy.
The Congress E-Newsletter is a special World Congress update is the issued quarterly to all people who have attended a world congress.
A special World Congress discounted registration fee is available every year for members attending the annual World Family Therapy Congress. Networking is one of the highlights of an IFTA Congress; many 2013 Congress attendees said, 'the best networking congress ever!"
An IFTA Member Directory is available to members only.
Joining IFTA is easy. Complete the application online or request an application by email. Become part of IFTA today!
Officers and Board of IFTAThe International Family Therapy Association has a distinguished Board of Directors and officers, all leaders in the family therapy field in their respective countries and recognized around the world.
Click here to meet your Board and officers.
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Tandem first jump training
Tandem jumping entered sport parachuting in the mid-1980's. Prior to that time, the only way to make a skydive was to jump alone the very first time. With the advent of tandem, it has become possible to train and jump with a highly experienced instructor on your first skydive. When you make your tandem jump, you will be attached with a specially trained and rated skydiver called a "tandem instructor". Your tandem instructor will wear a parachute system that has double-sized main and reserve parachutes designed to safely support both you and him. Since your tandem instructor is there to handle the skydive, the instruction required before your first tandem jump takes only fifteen minutes of your time, as opposed to the four to five hours necessary for our solo first jump program.
You and your tandem instructor will exit the jump aircraft from 10,000 feet above ground level and will freefall for approximately thirty seconds. During freefall, your position under the tandem instructor will give you the full sensation of freefall skydiving. To memorialize your leap from the heavens, you can hire a freefall photographer to capture you on video and still photos. Once the parachute is opened, you and your tandem instructor will jointly steer the canopy toward the landing area. During the four or five minute descent under the open parachute, you can have a thrilling ride with spirals, dives, and stalls-or a peaceful, gentle descent-according to your preference. After landing, your tandem instructor will give you a certificate acknowledging your accomplishment.
Tandem jumps are the easiest way to safely enjoy the experience of a freefall skydive on your first
jump. After your tandem jump if you wish to continue your training through our
Instructor Assisted Deployment method you may receive a discount on the solo first jump course.
If you are planning on making only one jump in your life or if you want to make a
jump to see if you would like to get into skydiving - tandem training is for you!
Call us at 316-776-1700 to schedule your tandem skydive!
Home, Tandem, IAD, Our Staff, Location, Contact Us, FAQs, How Safe Is Skydiving, Prices, News, Photo Gallery, Skydive Wichita, Skydive Wichita,


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craigasaurus necesitaba un nuevo diseño de página web y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 23 diseños de 9 diseñadores freelance.
Our new website is nearly ready to go but the homepage is refusing to come together in a cohesive look. All the required components (content) are there already, its just a matter of modifying and reworking them to look good.
Our current website is looking a bit tired (http://www.servantsasia.org) and so we have redesigned it in Joomla (http://www.servantsasia.org/sa) and most of it looks fine. BUT the homepage needs a bit of work. Every piece is already there, but they don't really go together design-wise.
Simpler design reworkings will be preferred because we don't want to do a major re-coding or overhaul.
As you can tell, we are going for a non-corporate, grassroots, urban and slightly grunge look (see here for examples of grunge design: http://www.smashingmagazine.com/200…e-design/).
- slightly grunge-look but not too much - keep all the components on the page
- a major reworking- the menus to change much- a corporate or high-tech look- web 2.0 look- stock photos of poor people - we have a policy of getting permission from people whose photos we use (not just the photogrpaher) - stock photos of objects or scenes are fine though


text:
GWAR Reveal Additional Acts for 2013 GWAR-B-Q + Release Brutal Commercial
Outer space metal act GWAR has just puked up more details concerning the fourth annual GWAR-B-Q. The 2013 edition of the GWAR-B-Q festivities has just doubled in size when it comes to bands and the intergalactic group have authorized a special commercial featuring an evil Slave Pit puppet to promote the event.
Along with GWAR, Municipal Waste, Corrosion of Conformity, Pig Destroyer, Cannabis Corpse and Loincloth, the fourth annual GWAR-B-Q will feature Warbeast, Battlecross, Wilson, Mobile Death Camp, Volture, Kung-Fu Dykes and a unique reunion by X-Cops, which featured Dave 'Oderus Urungus' Brockie and former GWAR members.
Tickets for the 2013 GWAR-B-Q will go on sale June 6 and standard tickets, deluxe tickets and premium tickets with be available for purchase. The $100 premium tickets include all-day entry for the event, an official GWAR-B-Q t-shirt, a DVD of the 2012 GWAR-B-Q and an exclusive laminate VIP pass for a 'Meat and Beat' signing with GWAR. Also, don't forget that you'll be able to gorge on GWAR's own GWAR-B-Q sauce and signature beer Impaled Ale throughout the fest!
"Once again we blow the horn of war and set into motion the gears that grind us towards the greatest GWAR-B-Q yet," says GWAR front-thing Oderus Urungus. "We will not only be rockin' like Dokken and slippin' the c-ck in, but we will shoving a wealth of GWAR products down your throat…not only our new GWAR-B-Q sauce, but our very own beer, Impaled Ale! Eat and drink yourself into a diabetic stupor, then puke it all up in the slam pit! It's gonna f—ing rule!"
The 2013 GWAR-B-Q will be held at Hadad's Water Park in Richmond, Va. on Aug. 17. Check out the festival's official commercial below and to buy tickets for the event, click here.
2013 GWAR-B-Q Commercial


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KYLE BLOUSE Something so 'in' for you to include in your basic outfit wardrobe. Never say no for this one that will surely get you look go extraordinary!
It features pleats at the front blouse for dramatic effect. A convenient blouse which it has wrapped buttons at both sides for wudhu' friendly & back zipper for closure.
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Finance, Office of the Director of / Economic Development Unit[Record group 62-7]
Agency Function
- The purpose of the Unit was to conduct studies and analyze economic data for use in the formulation of city policies and programs for short and long-term commercial and industrial development and to develop and maintain extensive data on specific urban problems to be used for basic economic analysis and forecasts of the state of the local economy. It was also intended to participate in the Program Planning Budgeting System, proposal writing for federal grants, and the initiation and implementation of special policies and programs.
- Agency History
- The Economic Development Unit was established in 1966 from a grant made by the Economic Development Administration of the United States Department of Commerce under Title M of the Economic Development Act of 1965. The head of the Unit was the City Economist who formerly was a member of the staff of the Development Coordinator's Office. After 1970, the City Economist duties were transferred to the Business Services Section of the Department of Commerce.
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System Requirements:
* Processor: 1 gigahertz (GHz) or faster
* RAM: 1 gigabyte (GB) (32-bit) or 2 GB (64-bit)
* Hard disk space: 16 GB (32-bit) or 20 GB (64-bit)
* Graphics card: MicrosoftDirectX 9 graphics device or higher
New Features:
* Swipe, slide, and zoom - Touch a full-powered PC. It's fast and it's fluid. Take natural, direct, hands-on control.
* Apps, front and center - Apps in Windows 8 work together to get things done faster. Get them from the Windows Store.
* Your Windows, everywhere - Windows 8 can connect you to your files, photos, people, and settings, wherever you sign in.
* Wall-to-wall web - Internet Explorer 10 Consumer Preview brings you immersive web browsing on screens big and small.
* The familiar, made better - Still devoted to your mouse and keyboard? Windows 8 makes the tried-and-true feel brand new.
* Download Windows 8
* Windows 8 FAQ
Official Microsoft themes, wallpapers and gadgets.
* Download Windows 7 Themes
* Download Windows 7 Wallpapers
* Download Windows 7Gadgets
By installing the Compatibility Pack along-side of Microsoft Office XP, or Office 2003, you will be able to open, edit, save, and create files using the formats that are used by Office 2010 and the 2007 Office system. Office 2000 users, as well as users of Windows 2000 SP4 and later, can convert Open XML Formats to binary file formats from within Windows Explorer.
* Download Microsoft Office Compatibility Pack
Windows Live Essentials 2011 includes:
1. Messenger (Windows, MSN)
2. Photo Gallery
3. Movie Maker
4. Windows Live Mesh (Keep photos and documents in sync between your computers, and on SkyDrive. Connect remotely to all of the files and programs on your PCs.)
5. Writer (Blog)
6. Family Safety (Parental control)
7. Mail (Hotmail, Live)
8. Messenger Companion (See and comment on links your friends have shared as you visit websites in Internet Explorer.)
9. Bing Bar (Search bar)
10. Outlook Connector Pack (Includes Microsoft Outlook Hotmail Connector and Outlook Social Connector Provider for Messenger.)
11. Microsoft Silverlight (Silverlight browser plug-in)
Windows 7 or Windows Vista with Service Pack 2.
* Download Windows Live Essentials 2011
Windows 7 Service Pack 1 adds support for Advanced Vector Extensions (AVX), a 256-bit instruction set extension for processors, and improves IKEv2 by adding additional identification fields such as E-mail ID to it. In addition, it adds support for Advanced Format 512e as well as additional Identity Federation Services. Windows 7 Service Pack 1 also resolves a bug related to HDMI audio and another related to printing XPS documents.
* Download Windows 7 Service Pack 1
Improvements in SP2:
1. Program compatibility.
2. Hardware support. SP2 provides you with additional support for Bluetooth wireless technology and improved performance for WiFi connections after the computer resumes from sleep mode. Additionally, you can now record data to Blu-ray Disc media.
3. General operating system updates. SP2 includes Windows Search 4.0, which has improved indexing and searching capabilities. SP2 also includes fixes that can improve recording TV using Windows Media Center.
* 32-bit Windows Vista Service Pack 2
* 64-bit Windows Vista Service Pack 2
A total of 1,174 fixes have been included in Windows XP Service Pack 3.
* Download Windows XP Service Pack 3
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Moderators: Three Stars, dagny, pfim, netwolf
shafnutz05 wrote:Niners defense has faded quite a bit in the last couple years
shafnutz05 wrote:They call every hard hit a helmet to helmet in the NFL now. It's absolutely pathetic
skullman80 wrote:and that will do it.
Seahawks came to play. 49ers didn't.
49ers need to take care of business against Arizona next week and they still win the division.
mac5155 wrote:http://deadspin.com/5970988/it-looks-like-joey-porter-will-be-spending-christmas-in-jail-on-felony-charges-for-allegedly-passing-bad-checks?utm_campaign=socialflow_deadspin_twitter&utm_source=deadspin_twitter&utm_medium=socialflow
Peezy will be spending Christmas behind bars.
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text: Jan Fabre is an established artist with a long rap sheet — having shown and made installations everywhere from The Royal Palace in Brussels to The Louvre Museum in Paris. It's impossible to pigeonhole him down into one medium, since he's worked with materials as diverse as bic ballpoint pens and beetle wings. Not to mention, he's also an author and theater director on top of everything else. If you happen to be lucky enough to be in the city of Antwerp from now until September 2012, you can view his sculpture installation entitled PIETAS at Park Spoor Noord. But if you go, don't forget to send us pictures! (via)


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My friend at Reboot the Republic recently directed my attention to the Continental Congress '09, which will be convening next week in Chicago.
Here is a press release with more information:
Citizens Rising in Defense of the Constitution
Citizens fed up with constitutional violations causing devastation to the economy and the Nation, are stepping up to join constitutional activist Robert L. Schulz, Chairman of We The People Foundation for Constitutional Education, to prepare for a modern-day Continental Congress to take place starting November 11, 2009 at the Pheasant Run Conference Center in St. Charles, Illinois. Continental Congress 2009 is not political or partisan and seeks to defend, not amend, the current Constitution.
Billed as the "next appropriate step for the free people of America," planners hope the historic national assembly will inspire the critical mass necessary to restore constitutional governance. 153 citizen-nominated-and-elected Delegates – three from each state and Washington, D.C. -- will convene for two weeks to methodically and factually document constitutional abuses which have taken place over many years, across many administrations, every branch of government and both parties. Delegates will consider specific non-violent, legal civic actions to stop the wrongs.
To help finance the historic event a .999 pure silver CC2009 commemorative round will be given for each donation of $100.00 or more. Planners are also seeking bigger donors they are calling the "patriotic, brave and wealthy" who "understand what Continental Congress can do for America." Furthermore, a money bomb is also in the works.
The decision to convene CC2009 comes after fourteen years of filing Petitions for Redress with the federal government for repeated violations of the Constitution by We The People Foundation for Constitutional Education, a national 501 c3 non-profit with headquarters in New York. Schulz says The Right to petition was put in the First Amendment by the Founding Fathers, "who did not want Americans to ever again experience a situation where their individual rights or civil liberties were threatened." To date, this Right has never been recognized by the government nor defined in any court of law. Schulz says it must now be used to hold elected officials accountable to the rest of the Constitution "which will shift the power from the government back to The People where it belongs."
"Americans are waking up to the urgent need to stand strong for the Founding Principles and our Constitution, as every violation further devastates our economy and our way of life," says Schulz. "We all desire the same outcome: restoration of our Nation. Continental Congress 2009 is the only strategy we see that is not political or partisan, does not rely on the electoral process, and can bring peaceful and legal solutions to our current situation. We hope this effort can be the catalyst that brings the freedom-loving people of America together at this critical time," he concludes.
Readers can learn more about CC2009, by going to www.cc2009.us
And I really recommend you watch this video:


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Not So Very High Tech
Today I spent the day giving a workshop for a small group of my colleagues. We all work at a large Dutch ICT company which implements and supports, among others, large scale scanning and imaging solutions.
One product in particular was the subject of today's workshop. Let's call the product 'World Class Capture' for the moment. Since 'World Class Capture' is indeed one of the leading vendors expectations of my, mostly younger, colleagues were high since this product must be High Tech! Probably expectations were a little bit too high afterwards.
When we entered the realm of the build-in scripting and programming possibilities I noticed a kind off disappointment. No web services? No .NET based assemblies? No beautiful Object Oriented interface? No Next High Tech Thing? No. Nope.
And they were right indeed. The only two build-in programming things in 'World Class Capture' are a pesky COM interface and a bloody ancient basic interpreter.
Since I have been working with this product, also among others, for the past seven years I did get used to the shortcomings and discrepancies. However, viewing things from a small distance again I wondered Why, What and How.
Why does 'World Class Capture' keep this old vehicle in the product? Well, suppose they would replace it with something High Tech! Than the answer is not too difficult. A lot of existing implementations would break after an upgrade.
What can 'World Class Capture' do to evolve to some New Technology? First of all, why should they? They have a large market share after all. So the product is good enough indeed. Maybe one day someone at 'World Class Capture' wakes up.
How should they change the product? Well. Keep it backwards compatible off course. Envisioning a new major version with the 'old' programming tools alongside some New High Tech Thing for the future is not that difficult, however.
For today to my colleagues and me it seems that a large part of today's real world software implementations aren't fancy high tech at all. Millions or billions of paper documents are scanned and processed using the assistance of some bloody ancient basic interpreter called Softbridge Basic Language.
With Kind Regards,
Aad Slingerland
The Netherlands


text:
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text:
by George Zamora
SOCORRO, N.M., November 26, 2003 – Dennis Schumacher, manager of the New Mexico Tech Residential Life Office, recently was honored by his colleagues in a regional professional organization by being awarded this year's Association of Intermountain Housing Offices (AIMHO) Distinguished Service Award.
Schumacher's commendation states that he was selected for the award on the basis of his "significant contributions to the Housing Program on [the New Mexico Tech] campus, demonstrated through outstanding job performance, loyalty, commitment, and consistency in going above and beyond expectations in assisting students, staff, and guests."
"Receiving this award was certainly nice," Schumacher says, "but having been nominated by my co-workers here at Tech was an even better personal tribute for me. The nomination alone meant more to me than whether or not I actually won the award."
In his role as manager of student housing and residential life at New Mexico Tech, Schumacher oversees a staff consisting of full-time office employees as well as Tech students who work part-time as resident assistants in their respective resident halls.
"Dennis' work as manager of Residential Life at New Mexico Tech has gone beyond providing safe and inviting living environments for students," says John R. Juarez, Tech's vice president for student and university relations. "His concern for students' health and well-being is well demonstrated in his proactive work with campus security and his ongoing collaborations with other university offices to ensure that Tech students fully enjoy the campus life experience."
Schumacher has been the manager of the New Mexico Tech Residential Life Office since March 1998.


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|South Pasadena, CA
|High School:
|South Pasadena HS
SENIOR (2010-11): Finished the season with a
career-best nine singles wins, compiling a 9-19 overall record and
6-16 mark in dual match play while competing in the middle of the
Titans' lineup... finished 3-7 at the No. 4 spot and 3-6 at the No.
5 spot while going 0-3 at the No. 3 position... posted a
season-best three-straight wins during the fall season en route to
the Flight C consolation singles title at the Matador
Invitational... also won the Flight C consolation doubles crown
with teammate Malorie dela Cruz that same weekend... posted
back-to-back singles wins twice during the spring... posted an 9-19
overall mark in doubles play with teammates dela Cruz, Katie
Nichols, and Summer Wallin... finished 6-9 at the No. 3 spot with
dela Cruz, and 8-10 overall, winning three-straight at one point in
the middle of April.
JUNIOR (2009-10): Finished the year 6-18
overall in singles play, competing mainly at the No. 6 singles
spot... had a solid fall with a 3-3 mark, including claiming the
Flight C consolation title at the Matador Invitational, winning
three straight matches... finished 4-17 overall in doubles play
with four different partners, competing in all but one dual match
at the No. 3 spot.
SOPHOMORE (2008-09): Finished 6-20 overall in singles play, including a 4-17 dual match record... played mainly in the middle of the lineup, posting an 0-3 mark at No. 2, a 3-10 record at No. 3 singles, and a 1-4 mark at the No. 4 position... teamed with five different partners in doubles play, going 5-18 combined... had her best doubles record with teammate Leslie Bullock, going 2-5 overall... only Titan to post more than one victory in Big West play, defeating Cal Poly's Amy Markhoff (1-0, ret.) and UC Riverside's Angelique Corpuz (6-4, 4-6, 14-12)... won back-to-back matches against Cal State Bakersfield's Kristi Jenkins (6-3, 6-4) and Markhoff... won both of her first-round matchups in the fall at the Fullerton Fall Intercollegiates and the Matador Invite.
FRESHMAN (2007-08): Finished second on the team with eight singles victories, posting an 8-17 overall record, including a 6-13 mark in dual matches... played mainly at the No. 3 spot, posting a 5-6 record while also seeing some time at the No. 4 position (0-4)... won three of her first four collegiate matches... won back-to-back matches three times on the year... won four of five matches at one point in March, posting victories over opponents from Cal State Bakersfield, Texas State, Westmont College, and Akron... posted a 6-19 overall record in doubles play with four different teammates... Spent most of the season at the No. 1 doubles spot with teammate Shelly Injejikian, going 3-11 in the top spot and finishing 3-14 overall.
Played her final three years at South Pasadena HS, earning MVP honors each season... Rio Hondo League champion as a senior... Ranked as high as No. 86 in the USTA 16-and-under rankings and No. 100 in the under-18 rankings... born in Almaty, Kazakhstan, but moved to the United States when she was eight years old.


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Albuquerque's Hip Hop Poet Laureate Speaks At Tech
SOCORRO, N.M. April 9, 2013 – New Mexico Tech welcomes the poet laureate of Albuquerque to campus for a workshop and poetry reading to commemorate National Poetry Month.
Hakim Bellamy, who recently released Swear, a new collection of poems, will give an tutorial brainstorming session with Tech students at 5:30 p.m. Wednesday, April 10, at the Skeen Library, followed by a poetry reading and book signing at 7 p.m. and reception with snacks. The event is free and open to all.
Hakim Bellamy and his son.
Hakim Bellamy and his son.
This event is sponsored by Academic Affairs and the Department of Communication, Liberal Arts, Social Sciences in celebration of April as National Poetry Month.
Bellamy, 34, is one of the new breed of poets. An African-American from Philadelphia, he developed a love of poetry from a childhood filled with hip-hop and other urban art forms.
"Some people think that hip hop takes our children away from literature," Bellamy said. "But for me, it took me to literature. My friends and I were rhyming and, to become better, I knew I had to have a better grasp of language."
Bellamy started performing as a youngster, but never called himself a poet until moving to Albuquerque in 2005. He quickly made a name for himself. He was a member of Team Albuquerque, the 2005 National Poetry Slam Champs in his first year of poetry slam. A year later as a member of Team UNM, he was the individual champ at the 2006 College Unions Poetry Slam Invitational Champs.
"Usually, poet laureates are at the end of their careers," Bellamy said. "This means a lot to me. It's nice to get recognition early in my career."
Bellamy has been teaching and performing for many years, but wasn't exactly raking in the dough. He had started considering getting an 8-to-5 job. He was sacrificing time with his family and wondering if there was a light at the end of the tunnel. Then, he was selected as Albuquerque's poet laureate.
"This is what I'm going to be," he said. "I'm going to be a poet – for better or worse."
Bellamy writes about a variety of topics, including New Mexico, race, and culture. His favorite topic, however, is his five-year-old son, who he calls his miracle.
He said his workshops are akin to brainstorming sessions. He'll ask participants to come up with topics and he'll encourage budding writers to develop novel approaches to standard topics.
"Love or depression … there's a million poems about those," he said. "How can we talk about it differently? How do we approach the topic? Maybe if I give my dog a voice – that's entertainment. … That's a good vehicle for your poem. If you can get that far, the poem writes itself."
He won the City Grand Slam Championship in Albuquerque (2005) and in Silver City (2008) as well as three consecutive University of New Mexico LOBOSLAM titles.
His poetry has been published in Albuquerque inner-city buses as a winner of the RouteWords Competition (2005). He's been published in the Harwood Anthology (2006), the Earthships Anthology (2007), Sin Fronteras Journal (2008), A Bigger Boat published by UNM Press (2008), Looking Back at Place (2008), Malpais Review (2011) and How to … .Multiple Perspectives on Creating a Garden, a Life, Relationships and Community (2011). In January of 2007, Bellamy was recognized as an honorable mention for the University of New Mexico Paul Bartlett Re Peace Prize for his work as a community organizer and journalist.
Hakim has acted for both stage and screen, and makes more music with his poetry nowadays as a songwriter, emcee and vocalist. Hakim was recently bestowed the populist honor of "Best Poet" by the Local iQ (Smart List) in 2010 and 2011 and by the Alibi (Best of Burque) for 2010, 2011, 2012 and 2013.
Hakim regularly works with, workshops and works for the many youth poets of this city via presentations at schools, non-profits and community organizations across the state of New Mexico. He recently left his position as Strategic Communications Director at the Media Literacy Project to devote himself full-time to his art. Visit Hakim at www.hakimbe.com.
Bellamy is the co-creator, along with Carlos Contreras and Colin "Diles" Hazelbaker, of the multimedia hip hop theater production Urban Verbs: Hip-Hop Conservatory & Theater, which has been staged throughout the country. He facilitates youth writing workshops for schools and community organizations in New Mexico and beyond. Having recently released his first book, Swear, Hakim is currently finishing his master's in communications and journalism at the University of New Mexico. He is founding president of Beyond Poetry LLC.
Bellamy's journey as a poet has come full circle as of late, wearing the hat of musician. He is equal parts musical artist, archivist and archeologist. He digs music. Piano lessons, recitals and many years singing in the church choir prepared him for the funk-infused, experimental-yet-melodic brand of hip hop that he produces today with creative partner Colin "Diles" Hazelbaker. In recent years, he's shared the musical stage with Zion-I, The Wailers, The Last Poets, Amiri Baraka, Cecil Taylor & Blacklisted Individuals.
As an actor, Bellamy has grown his talent on both the screen and the stage. He made a cameo in the independent film Romeo & Juliet Vs. The Living Dead (2009) and has made multiple appearances in local commercials (television and radio). He has done voice work for television and radio, worked for two years as a radio journalist at KUNM and has been cast as an extra in films and TV shows, such as Gamer (2009), Swing Vote (2009) and Crash the Television Series (2010).
On the stage he was cast in a major role in the 2009 Albuquerque production of The Exonerated, produced by NMRepeal.org. In the summer of 2011, he secured a major role as the Friar in the Vortex Theater's Summer Shakespeare Festival production of Romeo & Juliet for a 14-show run. He has since co-written and starred in Urban Verbs: Hip Hop Conservatory & Theater with his creative partner Carlos Contreras, currently booking tour dates for summer of 2013. He also has the lead role in the current Fusion Theater production of "Clybourne Park," which won the Tony, Pulitzer and Olivier awards in 2011.
By Thomas Guengerich/New Mexico Tech


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Erie Thames Powerlines Corporation is the wire services provider for Aylmer. They are responsible for ensuring that electricity reaches your home, and also read and maintain your meter
Moving to Aylmer
Are you moving to Aylmer and need to get electricity started under your name? First check that Erie Thames Powerlines Corporation provides electricity in your area at the regulated rate. Depending on where you live, your local utility may or may not be your regulated rate provider.
To find out whether Erie Thames Powerlines Corporation supplies your electricity, you can reach them by telephone at +1 519-485-1820
Starting Electricity Service in Aylmer
To open an account for your electricity supply, you will likely need to provide the following information:
- Your full name and contact information (phone number, email address)
- The Aylmer supply address (street address, postal code, city)
- Desired service start date
You may also be asked to provide some ID (such as your drivers licence number).
Erie Thames Powerlines Corporation Customer Service will be happy to answer any questions you might have about starting electricity service in Aylmer, including questions about electricity rates or billing.
Making an Appointment With Erie Thames Powerlines Corporation in Aylmer
If you need to have service reconnected to your property, to start up a new service line, or would like to move your meter, you should contact Erie Thames Powerlines Corporation at +1 519-485-1820 to learn more and to set up an appointment (if necessary).
Answers about Prices, Payments, and Bills at Erie Thames Powerlines Corporation Aylmer
If Erie Thames Powerlines Corporation provides your energy supply, you can contact them at +1 519-485-1820 for any questions about billing, payment options, or rates. If you purchase your energy from a company that is not responsible for your distribution (such as an energy marketer), you should contact them directly.
Find out more about your energy supply in Ontario
Nearby communitiesmalahide Distance: 2.2km
Township Of Malahide Distance: 4.8km
Municipality Of Central Elgin Distance: 7.7km
Central Elgin Distance: 11.3km
Sparta Distance: 11.3km


text:
It would have been simpler if MMS had been able to handle everything in the disease world, but there seems to be a fungus that MMS simply doesn't touch. In fact, MMS seems to feed the fungus. This fungus can occur in the the feet, or hands, or most any other place in the body.
It is not athlete's feet, it is much worse. All the athletes foot sprays and powders do not touch it. It can occur on the skin, and it seems to be much worse than any other skin infection. It itches and burns terribly and it appears to be under the skin as well as on the skin. It makes the skin slightly puff up, and it looks bad and it gets worse.
The name of this fungus has not been identified. It can last for years. I don't know if it has ever been fatal, but it seems to be very bad and sometimes it can get into the mouth and gums and cause much suffering. It also happens on the head or scalp where it causes havoc.
This particular fungus reacts to MMS with a stinging burning pain. It will almost always be worse after being treated with MMS. Your feet can be so bad that you cannot walk. This is the only way to identify it that I know of.
Luckily this particular fungus is rather rare. It does not happen in very many people. However, I have included it here as I do not know any other treatment than what I am about to tell of.
So if you have athlete's feet that will not go away, or gum disease that MMS will not cure in less than one week, or skin disease that just can't be treated successfully, then this is what to do, and don't worry, it won't do any harm.
Go to most any health food store and buy a jar of Aztec Clay. In foreign countries they have other clays that will work. All the clays for this purpose are Bentonite and in France it is called Montmorillonite. Mix this clay 50-50 with Vaseline (petroleum jelly). Then smear it on the various areas. If your problem is in the feet, smear it on the feet and cover it with thick socks. If it is on the skin, smear it on the entire area of the infection.
If you do not use the Vaseline it will not work very well. Vaseline makes it contact the skin and tissues and does something that makes it work much better. Only in the mouth would I not use the Vaseline and even there if it didn't work otherwise I would still use it. Just brush your teeth with the Aztec Clay powder just like using any particular tooth powder. Brush them gently, but three or four times a day.
The fungus infection should clear up in about one week. However, I would keep a light coating on for a month or so. This treatment has helped a number of people so far.
One more thing: There is always the possibility of getting a similar fungus infection inside the body. In that case, use the same clay inside the body by taking it by mouth. Start with 1 teaspoon of clay in ½ glass of water or juice and in several days work up to 2 tablespoon full's (rounded) a day.
Similarly, molecular silver solution that has produced very good results. Some people use this two hours after MMS doses to speed or supplement the MMS germicidal benefits. Molecular silver is superior to most colloidal silver because it is manufactured with high volages and is much higher in germicidal action. A search on the Internet will direct you to suppliers that have these products.
See also my web site called "MMSanswers.com" where about 150 diseases are listed together with a complied list of my email responses to questions about cancer and many other diseases.


text:
With its specially constructed chassis, high-performance engines and an array of top-quality standard equipment, the BMW X4 offers impressive agility.
You need the Adobe Flash Player in order to use some of the features of the BMW UK website. Please make sure that Flash is installed and activated. If you have not yet installed Flash, you can download it free of charge from the Adobe website.
Dynamic Damper Control
xDrive status


text:
Warning: this blog post will probably offend more people than normal. You should stop reading right now.
No doubt you've heard that Paypal has given Smashwords an ultimatum, saying it must remove all ebook titles that involve bestiality, rape-for-titillation, incest, and underage erotica.
Previously, several other online retailers also removed titles from their stores. Some didn't remove them, but made them harder to find by taking them off bestseller lists, keeping them out of various sales algorithms, etc.
Other bloggers have talked about this issue in-depth. I wanted to weigh in.
First of all, I think anyone who tells me what I can or can't do with my life, my body, or my mind is an asshole (as long as I'm not hurting anyone else) Keep your morals to yourself, or impose them on your defenseless children who will grow up feeling guilty and ashamed for good no reason.
Moral absolutism is silly. There is no black and white. There's always gray. And the gray keeps changing.
Throughout history, laws more or less coincided with the morals and values of the majority of any given population. Sometimes the ruling body of a country overrides what the people want, but this doesn't last forever.
Increasingly, the entities dictating morality are businesses. This is the current case with ebooks. Credit card companies pressure PayPal, who then pressures Smashwords. Ebook retailers don't like certain types of erotica, so they don't sell it.
This isn't censorship. It's businesses exercising their freedom to chose what they want to sell.
If you as an artist or consumer don't like that, too bad.
I don't like the fact that "In God We Trust" is written on my currency, but at least I have a legitimate gripe. The US Constitution grants freedom of religion, which includes freedom from religion. But the majority of my country is religious, so the phrase stays, unfair as it is.
It is not unfair if PayPal or Amazon doesn't want to be associated with "Daddy's BDSM Billy Goat Rape-A-Thon" (which, incidentally, I would probably buy, as long as the goat wasn't underage).
Though it isn't censorship, and it isn't unfair, I certainly do agree that it sucks. I'm not a fan of limiting choice. I dislike those who dictate morality. As a libertarian and a consumer, I should be able to get my smut where I want it, when I want it.
I also have other radical views. Gays should be able to marry. I should be able to buy liquor 24/7. Recreational drugs should be legal. Woman should be able to do whatever the hell they want to with their bodies (so should men.) If I want to die, I should be able to do so with dignity and without pain and get help if needed. I should be able to camp out in front of Wall Street for as long as I want to while protesting the wankers who screwed up my country's economy (and I say this as one of the hated 1%.) I shouldn't get fined for forgetting to wear my seatbelt. Victimless crimes should be abolished.
But these things aren't universally allowed in my country. That annoys me.
However, there are workarounds, and I'm still able to do the things I want to do. That's what I propose in this situation.
Porn is a billion dollar industry, and those smut-peddlers have found ways to make money online without PayPal.
Erotica authors should be able to do the same.
This isn't a set-back for authors. This is an opportunity to corner a huge market. If online retailers can't or won't sell "Spank My Donkey, Mommy: A Barely Legal Tale of Non-Consent" then artists need to stop complaining and figure out a way to sell it themselves.
There is an audience for this stuff. A big audience who is willing to pay. If the regular ebook outlets won't take their money, find a way to get it on your own. Hint: see what the porn sites are doing and copy them.
Authors aren't being censored. They aren't being treated unfairly. (If you want to know what being treated unfairly is like, sign a Big 6 contract.) So let's stop thinking in those terms.
Instead, think of how to exploit this situation for greater personal gain.
Supply and demand. There is a demand. Figure out how to supply it, without Amazon, B&N, Paypal, or Smashwords.
And when you do, contact me, so I have a place for my erotic masterpiece "Raped By Uncle Ostrich".
Here's the pitch:
Sexy Uncle Schlomo always made sixteen-year-old Betty Sue sopping wet with desire. So wet that she had to wear diapers.
But then a magic potion mix-up turns her hot uncle into a giant bird, and Betty's forbidden desire becomes even forbiddener.
When Uncle Ostrich gets high on marijuana and then marries Betty's own father (who then gets mangled in a car accident because he wasn't wearing his seatbelt, forcing Betty to smother Daddy to death in his hospital bed while riding his rock-hard manhood), devastated Uncle Ostrich begins raping everything in sight, and to poor Betty's delight she is next on the list.
This action is so hot it will melt your ereader! (Disclaimer: not responsible for melted ereaders.)
Also features spanking, abortion, toe-sucking, birding, elderly bondage, enema-play, cannibalism, erotic asphyxiation ending in murder, squirrel-play, injecting heroin into testicles, Pope-play, frotteurism, and irrumatio.
(Admit it, you had to Wikipedia those last two.)
So that's my take on the subject. Less complaining, more problem-solving. If some companies don't want to work with you, their loss.
All adult human beings are sluts. Figure out how to get our money.
Now I'm going to hop on Twitter and count the number of people who want to boycott or censor me. My current number followers is 8478. Let's see how many unfollow.
Addendum: The comments section is getting glutted with defining censorship. Allow me to clarify it here. If anyone disagrees with this definition, feel free to add to the comment thread.
Censorship is the suppression of speech or other public communication by a government, media outlet, or other controlling body.
(Since it came up in the comments: A media outlet is a publication or broadcast program that transmits feature stories and news to the public through various distribution channels.)
To suppress is to abolish, stop, put an end to, or withhold from disclosure or publication.
Is there any suppression going on by the government, media outlet, or a monopoly?
No. PayPal is not a monopoly, government, or media outlet.
PayPal is not preventing publication of erotica.
Hence PayPal's actions cannot be defined as censorship.
PayPal isn't infringing upon freedom of speech, freedom of the press, or human rights. They aren't censoring anything. They aren't suppressing anything. They're just refusing to sell certain titles.
Someone needs to point out a case where it is considered censorship when a retailer refuses to sell something.


text:
Annmclaren necesitaba un nuevo diseño de banner publicitario y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 36 diseños de 13 diseñadores freelance.
Ann McLaren http://www.annmclaren.net
I'm 55, from the UK and a multiple award-winning top producer with my home business. I'm looking for an eye-catching, professional banner that's not cheesy or gimmicky.
My target audience is in the UK.
It's a 60/40 split of male/female, aged 35 to 60.
Skilled working class or middle class.
Entrepreneurial streak. They are currently looking at offline business opportunities and franchises and don't realise (YET - this is where you come in!) that there are online business opportunities available which can create a better income for less investment than the ones they're looking at. The purpose of this banner is to alert them to this fact!
I need 1 animated banner and 1 non-animated (same or similar design) each in 2 sizes - 750 x 550 and 800 x 600.
This is for a UK-based pop-up campaign on targeted URLs. What this means is that a visitor has clicked to visit a site like this:
and in front of that site a 750 x 550 or 800 x 600 window will pop-up containing my banner, which will be linked to my website http://www.annmclaren.net
So, because the viewer was not expecting to see my banner, it's vital that it grabs their attention IMMEDIATELY and sells them on the benefits of clicking on it.
The colours of the banner need to match the site they're on (i.e. http://uk.businessesforsale.com) and the site I am taking them to http://www.annmclaren.net.
For a headline be creative but I'm thinking along the lines of "Starting A Business Is Risky. Discover the Income Producer And Beat The Recession".
A file is attached with some bullet points for ideas.
I need my name Ann McLaren or image (or both) on the banner and a prominent call to action button, i.e. Learn More or Click Here.
The perfect banner will make them think "I didn't know about this but it sounds better than what I was looking for".


text:
Washington got back to playing baseball and swept Atlanta to keep the Braves from clinching the NL East. Meanwhile, the AL wild-card race remained tight with Texas beating Tampa Bay. A quick look at baseball's playoff picture:
TIGHTENING RACE: Four of the six teams vying for the two AL wild-card berths squared off against a fellow contender Tuesday night. Texas topped Tampa Bay 7-1 to move into a tie with the Rays atop the wild-card chase. Cleveland moved a half-game out while Baltimore is now within two games. Kansas City and New York both lost, but are still in the hunt 3½ games back.
STAYING HOT: The Washington Nationals swept Atlanta in a day-night doubleheader to postpone the Braves' first NL East title since 2005. Washington has won 10 of its last 11 games to close within five games of Cincinnati for the NL's second wild-card berth. The first game was a makeup following shootings at the nearby Washington Navy Yard on Monday. Rookie Tanner Roark (7-0) remained unbeaten by allowing just two hits in seven shutout innings in the nightcap. Manager Davey Johnson even seemed to make up a word — "posure," apparently a cross between poise and composure — to describe his young star.
WELCOME BACK MATT: Matt Kemp went 4 for 4 with three RBIs in his first start since coming off the disabled list Monday to lead Los Angeles to an easy 9-3 rout of Arizona. With the victory, the Dodgers reduced their magic number to clinch the division to two. They can clinch the NL West with a win Wednesday.
LOST PERFECTION: Boston closer Koji Uehara saw his run of 37 consecutive batters retired come to an end as the Orioles rallied against the Red Sox's star closer. Danny Valencia ended that streak with a triple to lead off the ninth and Matt Wieters hit a go-ahead sacrifice fly to give Baltimore the 3-2 win. Uehara fell four outs short of Bobby Jenks' major league record for a reliever of 41 consecutive retired batters set in 2007 and Mark Buehrle's mark for all pitchers of 45 in 2009. The run was the first off Uehara in 30 2-3 innings and ended a streak of 27 scoreless outings since July 9.
SLUMPING PIRATES: Pittsburgh can't wait for San Diego to get out of town. The Padres beat the Pirates for the second straight night, improving to 30-10 in Pittsburgh since the new ballpark opened in 2001. Pittsburgh fell a game behind St. Louis, which routed Colorado 11-4 to take the NL Central lead.
Copyright 2013 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


text:
We've had Johnny and June in "Walk the Line," Katharine Hepburn and Henry Fonda's grouchy old-timers in "On Golden Pond" and real-life marrieds Elizabeth Taylor and Richard Burton bickering their way through "Who's Afraid of Virginia Woolf?"
But they've been the exceptions. Usually, when it comes to Oscar couplings, the academy seems to prefer the sizzle of first love to the monotony of matrimonial nesting. This year, however, for better or worse, it don't mean a thing if it ain't got that ring. From the royals in "The King's Speech" to the moms in "The Kids Are All Right," this year's Oscar pairs have been to the altar and back.
And, if we're to glean anything from this crop of couples, it's that, whether you're battling Scooter Libby or the advances of your sperm-donor kids' sexy biological dad, marriage is a train that doesn't make it back to the station without leaving a little blood on the tracks. Caution: Minor plot spoilers ahead.
The marriage: King George VI ( Colin Firth), a.k.a. Bertie, and Elizabeth ( Helena Bonham Carter), a.k.a. the future Queen Mum.
The message: Bonham Carter says she decided to play Elizabeth as a "marshmallow made with a welding machine." Elizabeth still believes George can beat his crippling stammer long after the future king has thrown in the towel. She reassures him through his self-doubt and, when they finally find an effective speech therapist, she has the self-knowledge to step back and let George develop a close relationship (an early bromance, if you will) with said therapist without becoming jealous.
"We live in a world where people emphasize development of the self," "Speech" director Tom Hooper says. "It's me, me, me. And, actually, this film says that sometimes you can achieve that best version of yourself by opening up to other people and letting them in."
Will they make it? Elizabeth and George remained married until George's death in 1952.
The marriage: Becca ( Nicole Kidman) and Howie ( Aaron Eckhart) had a storybook union — great Westchester house, great sex to the musical stylings of Al Green — until the accidental death of their 4-year-old son.
The message: Most fictional tragedies occur because of some kind of tragic flaw. That's not the case here, which might make this couple's challenge even greater. How do you stay together in the wake of an inexplicable, irreparable loss? Howie wants to hold on to his son's memory; Becca needs to start anew.
"What I love about the writing is that they're both right and both wrong," says director John Cameron Mitchell. "That's why it's not easy. There's no right way or wrong way here. The question is, can they find a way together?"
Will they make it? "My Scottish granny used to say, 'Love me little, but love me long. Because little and often fills the purse,'" Mitchell says. "Howie and Becca had that early passion, but now, through this, they've forged something deeper. When she takes his hand at the end, it still gets me."
The marriage: Groovy earth mama Jules ( Julianne Moore) and perfectionist doc Nic ( Annette Bening) have raised two teenagers, remodeled a cool Venice house and lived to tell the tale. But the sudden arrival of their kids' sperm-donor dad forces them to examine aspects of their marriage they've long ignored.
The message: It doesn't matter whether you're straight or gay or shop at Trader Joe's or Stater Bros., if you put day-to-day living ahead of your partner, you're screwed.
"When you're trying to keep a house together and keep the kids together and just get through life, it's easy to start feeling unseen and unappreciated," says "Kids" director Lisa Cholodenko, who has a 4-year-old with her longtime partner, Wendy Melvoin. "Life is chaotic and, no, you can't analyze everything or you'd be on the couch all day. But every once in a while, yeah, you need to take inventory."
Will they make it? Jules does stray, but it was … you know … the father of her child. She was confused and, yes, turned on. But it's in the past, and Nic seems to have forgiven her.
The marriage: Dean ( Ryan Gosling) and Cindy ( Michelle Williams) enjoy a charming courtship involving old-timers and ukuleles. A few years later, they're married, miserable and having bad, drunken sex in the world's worst motel room. Where did the love go?
The message: Director Derek Cianfrance sees "Blue Valentine" as a cautionary tale about the erosive — and corrosive — power of time.
"When they started out, they were two fish in the ocean," Cianfrance says. "Now, as time has passed, they're fish in a bucket, trying to get to the ocean of opportunity again."
Adds Gosling: "There's some deep problems, but they're not aware of them. Before I did the film, I asked a couple of friends — since divorced — what their biggest problems were with one another. She piped up: 'He never squeezes out the sponge.' She was livid about it, convinced there was some ulterior motive. Obviously, they had big issues, but the sponge was taking all the heat."
Will they make it? Cianfrance says the "movie doesn't end. It stops." Williams hopes it was "just two bad days in the marriage" and that they can start anew. Maybe. But watch the movie. Those are two really bad days.
The marriage: CIA operations officer Valerie Plame ( Naomi Watts) and former U.S. ambassador Joseph C. Wilson IV ( Sean Penn) deal with the fallout of the public disclosure of Plame's profession.
The message: Opposites attract. When Wolf Blitzer interviewed Plame and Wilson shortly before the film opened, he played word-association. First name: Dick Cheney. Plame gave a long, measured response. Wilson simply said, " Traitor." Next name: I. Lewis "Scooter" Libby. Again, Plame answered thoughtfully and at length. And, again, Wilson kept his response succinct: "Traitor."
"They really are the odd couple," director Doug Liman says. "The first time they saw the movie, Joe's bawling his eyes out. And Valerie is sitting there stone-faced. She turns to Joe, and says, 'Knock it off. Get it together.' That's their relationship in a nutshell."
Will they make it? Well, they did survive Cheney.
We've had Johnny and June in "Walk the Line," Katharine Hepburn and Henry Fonda's grouchy old-timers in "On Golden Pond" and real-life marrieds Elizabeth Taylor and Richard Burton bickering their way through "Who's Afraid of Virginia Woolf?"


text:
Search results for: R 1136 Block 19 F B Area
R 1136 Block 19 F B Area
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Webotopia provides a business directory designed to promote R 1136 Block 19 F B Area companies, products and services whilst helping it's users find the R 1136 Block 19 F B Area information they need. Webotopia business directory, with the help of it's dedicated support specialists and reviewers, enables delivery of R 1136 Block 19 F B Area quality content, excellent customer support as well as value added services such as content publishing and press releases for it's users.


text:
Meg Whitman's former housekeeper was an undocumented worker and claims working for the Republican candidate for Governor was a "nightmare."
That all changed, Allred says, when Whitman launched her campaign for Governor, realizing having an illegal alien on board was a liability ... and fired her.
Santillan says Whitman treated her "like a piece of garbage" by letting her go.
Allred says Santillan is filing a claim with the Labor Commission for back wages and mileage.
Yesterday, Whitman said in the debate with Jerry Brown that employers should be held accountable for hiring undocumented workers.
Click here to read Gloria's written statement.
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