(A1) Identification of oral health care needs in children and adults, management of oral diseases.
The workshop considered five questions reviewing the identification of international oral health care needs of children and adults, and the management of oral diseases in resource-poor countries: (1) What is the role of the dental profession in the management of the HIV-infected individual? (2) Identifying health care needs-What are the epidemiology and disparities of HIV-associated oral lesions in children from different continents? (3) How effective is HIV treatment in controlling oral diseases? (4) Could we develop basic inexpensive oral and dental care protocols for economically deprived HIV-infected patients? and (5) What is the best method of arranging resources to meet the oral health care needs of people with HIV disease? The consensus of the workshop participants was that there is a need to re-target research efforts to non-established market economy countries and prioritize research in these regions to children with HIV disease. It will be important to assess commonalities and variations in oral health needs across geographical and cultural boundaries, and research efforts should be centralized in resource-poor countries to support multi-center longitudinal standardized studies. It is essential that oral health research be integrated into other health care research programs, to make these research priorities and public health initiatives feasible.


Variation in CR imaging plate readers.
This study investigated computed radiography (CR) image processing to determine whether variation exists within and among CR imaging plate readers. Photostimulable imaging plates were exposed using a phantom test tool and processed in 4 CR readers located in diverse settings in an urban academic medical center. Research results indicate daily variation of S-numbers within individual CR readers did not exceed tolerance limits, although over the 3-week study period, evidence of S-number variation within individual CR readers was mixed. In addition, S-number variability among multiple CR readers was found to be statistically significant. Although the cause of the variability remains unknown, evidence of variability among multiple CR readers indicates a need for systematic quality control.


Revisiting evidence for widespread seismicity in the upper mantle under Los Angeles.
We revisit the finding of widespread deep seismicity in the upper mantle imaged with a dense, temporary nodal seismic array in Long Beach, California using back-projection to detect candidate events and trace randomization to develop a reliable imaging threshold for candidate detections. We find that nearly all detections of small events at depths greater than 20 kilometers in the upper mantle fall below the reliability threshold. We find a modest number of small, shallower events in the crust that appear to align with the active Newport-Inglewood Fault. These events occur primarily at 15- to 20-kilometer depth near the base of the seismogenic zone. Localized seismicity under fault zones suggests that the deep extensions of active faults are localized and deforming, with stress concentration leading to a concentration of small events, near the seismic-aseismic transition.


Minocycline Pretreatment Prevents Blood-Brain Barrier Disruption in Septic Rats.
The aim of the study was to explore the mechanism by which minocycline protects the blood-brain barrier (BBB) in septic rats. A sepsis rat model was generated in healthy, male Sprague-Dawley rats by cecal ligation and puncture (CLP). The rats were randomly divided into four groups and treated as follows: sham-operated plus normal saline (Sham + S group), CLP plus normal saline (CLP + S group), CLP plus minocycline pretreatment (CLP + M1 group), and CLP plus minocycline treatment (CLP + M2 group). Rats in the CLP + M1 group received 45 mg/kg minocycline by intraperitoneal injection every 12 h for 72 h. Rats in the Sham + S and CLP + S groups were injected with the same volume of normal saline every 12 h for 72 h. Rats in the CLP + M2 group were intraperitoneally injected with 45 mg/kg minocycline immediately after CLP and once every 12 h for 72 h. All rats were sacrificed at 72 h after operation. Tumor necrosis factor α and interleukin 6 levels, the expression of ionized calcium-binding adaptor molecule-1 (Iba-1), and the permeability of the BBB were measured. The expression of matrix metalloproteinases-9 (MMP-9) and the tight junction proteins zonula occludens-1 (ZO-1) and occludin was detected by Western blot. In addition, Evans blue (EB) staining, immunohistochemistry, and ELISA analysis were carried out. Minocycline pretreatment significantly inhibited microglial activation, decreased the sepsis-induced expression of MMP-9, increased the expression of ZO-1 and occludin, and improved the permeability of the BBB. Minocycline treatment failed to inhibit microglial activation, decrease the sepsis-induced expression of MMP-9, increase the expression of ZO-1 or occluding, or improve the permeability of the BBB. Minocycline pretreatment can effectively improve the altered permeability of the BBB caused by sepsis. The mechanism may be related to the inhibition of microglial activation and MMP-9 expression and increased expression of ZO-1 and occludin.


Template-free, controllable and scalable synthesis of poly(5-aminoindole) nanoparticles for printable electrochemical immunosensor with ultra-high sensitivity.
Conductive polymer polyindole derivatives have good conductivities and abundant functional groups, which would offer great potential for versatile applications including biosensors, bioelectronics and energy devices. However, the polyindole derivatives are mainly synthesized by the electropolymerization method on conductive electrode surfaces, which limits large-scale synthesis and practical applications. Herein, we explore a strategy of template-free, controllable and scalable synthesis of poly-5-aminoindole (PIn-5-NH2) nanoparticles (NPs) and demonstrate the application of PIn-5-NH2 NPs in printable multiplexed electrochemical biosensors with ultra-high sensitivity. The synthesis of PIn-5-NH2 NPs is based on a self-templated method since the In-5-NH2 monomer with amphiphilic structures can form micelles by self-assembly in an aqueous solution. The diameter of PIn-5-NH2 NPs could be controlled by adjusting the synthesis conditions, such as monomer concentration, oxidant/monomer ratio and reaction time. The PIn-5-NH2 NPs possess distinct features, including good conductivity, large surface area, and abundant -NH2 functional groups for covalent binding of the antibody, and therefore offer substantial possibilities for developing an all-printable process to fabricate multiplexed electrochemical immunosensors. The printed multiplexed electrochemical immunosensors on the basis of the aqueous suspension of PIn-5-NH2 NPs linked with antibodies can simultaneously detect multiple cancer markers, and exhibit high sensitivity and good selectivity. Our facile and scalable synthesis strategy would offer great opportunities for versatile applications of PIn-5-NH2 NPs.


[Temperature effect on the noninvasive measurement of human blood glucose by NIR].
During the noninvasive measurements of body blood glucose, the result will be effected by many factors, such as the measuring conditions including temperature, contact pressure and so on, and in addition the change of body's state also will induce some error. However, among so many factors the temperature is very important and should be discussed. To find the quantitative value of the result bias caused by temperature in the wavelength range from 1 100 to 1 700 nm, the aqueous glucose with the concentration ranging from 10 mg x dL(-1) to 200 mg x dL(-1) and 10 mg x dL(-1) interval was detected at temperature of 15, 20, 25, 30, 35 and 40 degrees C. Then six different models at different temperature were founded and predicted one another. The maximum RMSEP result of models is 11. 227 9 mg x dL(-1) and the minimum is 3. 298 8 mg x dL(-1). The correlation is about 0.98. The authors have also found that 1 degrees C change of temperature will induce deltac = 2.662 (mg x dL(-1) x degrees C(-1)) change of the prediction result, so these show that the detect error will be minimal when the temperature of measuring is the same as that of modeling. Moreover, the authors put forward two approaches to decreasing or compensating the error induced by the temperature.


Pulsatile neck mass: A rare cause.
A 20-year-old female presented with a pulsatile neck mass. On evaluation, she was found to have right cervical aortic arch, which is a rare anomaly. We highlight the conventional and Computed tomography angiography features of this vascular anomaly.


Geographic variation in resource dominance-discovery in Brazilian ant communities.
A predictive framework for the ecology of species invasions requires that we learn what limits successful invaders in their native range. The red imported fire ant (Solenopsis invicta) is invasive in the United States, Puerto Rico, Australia, New Zealand, and China. Solenopsis invicta appears to be a superior competitor in its introduced range, where it can cause the local extirpation of native species, but little is known about its competitive ability in its native range in South America. Here we examine the competitive ability of S. invicta for food resources in three widely separated Brazilian ant communities. Each of these communities contains 20-40 ant species, 8-10 of which were common and frequently interacted with S. invicta. S. invicta at all three sites was attacked by several species-specific phorid parasitoids, and at one site, two other species were attacked by their own specialized parasitoids. We examined interactions in these local communities for evidence that trade-offs among ant species between resource dominance and resource discovery, and between resource dominance and parasitoid vulnerability facilitate local coexistence. The trade-off between resource dominance and resource discovery was strong and significant only at Santa Genebra, where parasitoids had no effect on the outcome of confrontations at resources. At Bonito, parasitoids significantly reduced the ability of S. invicta, which was the top-ranked behavioral dominant, from defending and usurping food resources from subordinate species. In the Pantanal, S. invicta ranked behind three other ant species in a linear hierarchy of behavioral dominance, and lost the majority of its interactions with a fourth more subordinate species, Paratrechina fulva, another invasive species. Parasitoids of S. invicta were uncommon in the Pantanal, and did not affect its low position in the hierarchy relative to the other two sites. Parasitoids, however, did affect the ability of Linepithema angulatum, the top-ranked behavioral dominant in this community, from defending and usurping resources from behavioral subordinates. These results indicate that both interspecific competition and trait-mediated indirect effects of phorid parasitoids affect the ecological success of the red imported fire ant in its native range, but that the relative importance of these factors varies geographically.


[Long-term results of surgical treatment for achalasia of the esophagus].
25 patients were operated with Heller's procedure for achalasia of the esophagus. A study of their clinical, radiological, manopetric and pH-metric evolution was carried out over an average period of 75 months. While the long-term results were satisfactory in 80% of these patients, the poor results were essentially caused by the occurrence of gastroesophageal reflux. An objective study performed by manometry and pH-metry allowed evidencing true asymptomatic acid reflux. It is necessary to devise an anti-reflux valve if the myotomy reaches as far as the cardia.


Patch testing in children.
92 children (45 girls, 47 boys), mean age 9.3 years (3-14.75), were referred to the Contact Dermatitis Investigation Unit, Belvidere Hospital, Glasgow, for patch testing during the period 1979-93 for the investigation of allergic contact dermatitis (ACD). The diagnoses at the time of referral were atopic dermatitis (45), non-atopic with localized dermatitis (26), juvenile plantar dermatosis (15), orofacial granulomatosis (2), vaccination reaction (2) and atypical psoriasis (2). In total, there were 55 positive reactions in 30 children. The commonest allergens were metals (18), fragrances (11) and rubber compounds (6). The patient groups with the highest yield of positive patch tests were those patients with atopic dermatitis who had a good history of a precipitating contact factor (4/5), and non-atopic patients with dermatitis of hand and/or feet (7/14). Our findings suggest that allergic contact dermatitis is more common in children than generally appreciated and that patch testing is a practicable and clinically worthwhile procedure in children.


Investigating the association between OPA1 polymorphisms and glaucoma: comparison between normal tension and high tension primary open angle glaucoma.
OPA1, the gene responsible for autosomal dominant optic atrophy, represents a good candidate gene for glaucoma, as there are similarities in the clinical phenotype and OPA1 is expressed in the optic nerve. Single nucleotide polymorphisms on intervening sequence (IVS) 8 of the OPA1gene (genotype IVS8+4 C/T;+32T/C) were recently found to be strongly associated with normal tension glaucoma (NTG). In order to investigate whether this association exists in patients with high-tension glaucoma (HTG), 90 well-characterized HTG patients were examined for the presence of these OPA1polymorphisms by PCR amplification followed by bi-directional sequencing. Five out of 90 HTG subjects (5.6%; 95% CI 1.8-12.5) were found to carry the OPA1 genotype IVS 8+4 C/T; +32 T/C, compared with 32/163 (19.6%; 95% CI 13.8-26.6) NTG subjects [chi(2)=9.2, P=0.002, OR 4.1 (95% CI 1.6-11.1)], and 7/186 (3.8%; 95% CI 1.5-7.6) control subjects [chi(2)=0.47, P=0.49, OR 1.5 (95% CI 0.5-4.9)]. These results indicate that unlike NTG, the OPA1 genotype IVS8+4 C/T,+32T/C is not significantly associated with high-tension primary open angle glaucoma, and suggest genetic heterogeneity between the conditions.


Loss of Par-1a/MARK3/C-TAK1 kinase leads to reduced adiposity, resistance to hepatic steatosis, and defective gluconeogenesis.
Par-1 is an evolutionarily conserved protein kinase required for polarity in worms, flies, frogs, and mammals. The mammalian Par-1 family consists of four members. Knockout studies of mice implicate Par-1b/MARK2/EMK in regulating fertility, immune homeostasis, learning, and memory as well as adiposity, insulin hypersensitivity, and glucose metabolism. Here, we report phenotypes of mice null for a second family member (Par-1a/MARK3/C-TAK1) that exhibit increased energy expenditure, reduced adiposity with unaltered glucose handling, and normal insulin sensitivity. Knockout mice were protected against high-fat diet-induced obesity and displayed attenuated weight gain, complete resistance to hepatic steatosis, and improved glucose handling with decreased insulin secretion. Overnight starvation led to complete hepatic glycogen depletion, associated hypoketotic hypoglycemia, increased hepatocellular autophagy, and increased glycogen synthase levels in Par-1a(-/-) but not in control or Par-1b(-/-) mice. The intercrossing of Par-1a(-/-) with Par-1b(-/-) mice revealed that at least one of the four alleles is necessary for embryonic survival. The severity of phenotypes followed a rank order, whereby the loss of one Par-1b allele in Par-1a(-/-) mice conveyed milder phenotypes than the loss of one Par-1a allele in Par-1b(-/-) mice. Thus, although Par-1a and Par-1b can compensate for one another during embryogenesis, their individual disruption gives rise to distinct metabolic phenotypes in adult mice.


Verticillarone: a new seco-fusicoccane diterpenoid ketonepoxide from Hypoestes verticillaris.
The aerial parts of Hypoestes verticillaris (L.F.) Sol. (Acanthaceae) has afforded a new seco-fusicoccane diterpenoid ketonepoxide, which was characterized as 1(10) seco-fusicocc-3 (4)-ene-5, 11, 14-trione-8 (9), 1 (7)-diepoxide (1) on the basis of spectral analysis and named as verticillarone, along with earlier reported diterpenoid, i.e. 13-hydroxy-7-oxo-labda-8, 14-diene (2).


Prison health care: work environment and the nursing role.
This article, the first in a new series on prison nursing, gives an overview of the world of prison nursing and the environmental factors that make it so different from other types of nursing. It welcomes the recommendation in the report 'Patient or Prisoner: A New Strategy for Health Care in Prisons' (HM Chief Inspector of Prisons for England and Wales, 1996) that the NHS should be responsible for providing health care for prisoners and the recent publication of the report of the Joint Prison Service/NHS Executive Working Group 'Future Organization of Prison health Care' (Department of Health, 1999), which endorses a formal partnership between the NHS and prison service. The article highlights the benefits that this will have for prison nurses who often feel professionally isolated. The role of the nurse working in the prison healthcare environment is outlined. The work of prison nurses differs from the role of nurses working in other healthcare situations as it is controlled by environmental factors associated with regimes, security and the prison culture. However, changes are taking place and a closer link with local services is envisaged. These changes are an excellent opportunity for nurses and nursing to establish a clear role in prisons and to develop further healthcare standards in the prison service.


Percutaneous Reduction of Displaced Radial Neck Fractures Achieves Better Results Compared With Fractures Treated by Open Reduction.
There is uniform agreement in the literature that radial neck fracture with <30 degrees of angulation and minimal translation in younger children can be managed by casting alone, without the need for closed reduction. For more severe fractures, closed manipulation techniques followed by percutaneous reduction technique (in case closed reduction fails) should be performed before switching to an open reduction technique. Debate still exists regarding the best treatment protocol for severely displaced fractures. Although some surgeons favor open reduction with the argument that repeat manipulations may cause iatrogenic injuries, and stiffness, others believe that open reduction is one of the most contributing factors for poor outcome. On the basis of this paradigm, we present our treatment algorithm based on the data demonstrating that percutaneous reduction of displaced radial neck fractures achieve better results than open reduction.


Traffic Crash Characteristics in Shenzhen, China from 2014 to 2016.
Road traffic crashes cause fatalities and injuries of both drivers/passengers in vehicles and pedestrians outside, thus challenge public health especially in big cities in developing countries like China. Previous efforts mainly focus on a specific crash type or causation to examine the crash characteristics in China while lacking the characteristics of various crash types, factors, and the interplay between them. This study investigated the crash characteristics in Shenzhen, one of the biggest four cities in China, based on the police-reported crashes from 2014 to 2016. The descriptive characteristics were reported in detail with respect to each of the crash attributes. Based on the recorded crash locations, the land-use pattern was obtained as one of the attributes for each crash. Then, the relationship between the attributes in motor-vehicle-involved crashes was examined using the Bayesian network analysis. We revealed the distinct crash characteristics observed between the examined levels of each attribute, as well the interplay between the attributes. This study provides an insight into the crash characteristics in Shenzhen, which would help understand the driving behavior of Chinese drivers, identify the traffic safety problems, guide the research focuses on advanced driver assistance systems (ADASs) and traffic management countermeasures in China.


Coloured overlays and their effects on reading speed: a review.
Coloured overlays can reduce symptoms of visual stress and improve reading speed. These benefits are reliable and are not attributable simply to placebo effects. Five percent of children in mainstream education read at least 25% more quickly with an overlay, provided they have chosen the colour. The suboptimal design of children's text and the high level of classroom lighting may be partly responsible.


Dexmedetomidine: What's New for Pediatrics? A Narrative Review.
Over the past few years, despite the lack of approved pediatric labelling, dexmedetomidine's (DEX) use has become more prevalent in pediatric clinical practice as well as in research trials. Its respiratory-sparing effects and bioavailability by various routes are only some of the valued features of DEX. In recent years the potential organ-protective effects of DEX, with the possibility for preserving neurocognitive function, has put it in the forefront of clinical and bench research. This comprehensive review focused on the pediatric literature but presents relevant, supporting adult and animal studies in order to detail the recent growing body of literature around the pharmacology, end-organ effects, organ-protective effects, alternative routes of administration, synergetic effects, and clinical applications, with considerations for the future.


Aquaporin-4 immuneglobulin g testing in 36 consecutive jamaican patients with inflammatory central nervous system demyelinating disease.
Epidemiological studies of neuromyelitis optica (NMO) in Jamaica are lacking. Here we reviewed the clinical records of 700 patients undergoing neurological evaluation at the Kingston Public Hospital, the largest tertiary institution in Jamaica over a 4 month period. We investigated the diagnostic utility of Aquaporin-4 ImmuneglobulinG (AQP4-IgG) testing in 36 consecutive patients with a diagnosis of an inflammatory demyelinating disorder (IDD) of the central nervous system (CNS). Patients were classified into 3 categories: i) NMO, n=10; ii) multiple sclerosis (MS), n=14 and iii) unclassified IDD (n=12). All sera were tested for AQP-IgG status by cell binding assay (Euroimmun). No MS cases were positive. Ninety per cent of NMO cases were positive. Four of 12 patients with unclassified IDD tested positive for AQP4-IgG. AQP4-IgG seropositivity was associated with a lower socioeconomic status, higher EDSS (P=0.04) and lower pulmonary function than the seronegative cases (P=0.007). Aquaporin-4 autoimmunity may account for a significant proportion of Jamaican CNS IDDs.


[Effect of electromagnetic field produced by mobile phones on the activity of superoxide dismutase (SOD-1) and the level of malonyldialdehyde (MDA)--in vitro study].
The aim of the study was to assess in vitro the effect of electromagnetic field produced by mobile phones on the activity of superoxide dismutase (SOD-1) and the level of malonyldialdehyde (MDA) in human blood platelets. The suspension of blood platelets was exposed to the electromagnetic field with the frequency of 900 MHz for 1, 3, 5, and 7 min. Our studies demonstrated that microwaves produced by mobile phones significantly depleted SOD-1 activity after 1, 5, and 7 min of exposure and increased after 3 min in comparison with the control test. There was a significant increase in the concentration of MDA after 1, 5, and 7 min and decrease after 3 min of exposure as compared with the control test. On the grounds of our results we conclude that oxidative stress after exposure to microwaves may be the reason for many adverse changes in cells and may cause a number of systemic disturbances in the human body.


Genetic immunization maps T cell (auto)immune responses to self antigens homologous to exogenous proteins.
Genetic immunization represents a new tool for investigating physiological and pathological immune responses. Here we used genetic immunization with naked DNA to study the immune relevance of aminoacid sequence homologies by evaluating the outcome of immunization to a viral protein homologous to an HLA molecule. The viral protein was Balf2, a protein of Epstein-Barr virus (EBV) that shares aminoacid sequence homology with the HLA allele DRB1*0801. After genetic immunization of BALB/c mice with a construct encoding Balf2, we analyzed T cell responses of immunized mice. We found that cross-reactive proliferative and cytotoxic responses were raised to the homologous sequence as expressed by HLA-DRB1*0801. Furthermore, preferential secretion of Th1-type pro-inflammatory cytokines occurred. This strategy can allow rapid screening of interactive immune networks involving aminoacid sequence homologies between organisms.


Current status of Geriatric Dentistry Educational Activities in U.S. dental schools.
The current status of Geriatric Dentistry Educational Activities (GDEA) in U.S. dental schools is reported in this survey. Data were collected regarding faculty involvement in geriatric programs, didactic and clinical academic curricula, and other aspects of the ongoing activities in schools. Data were reported by 50 (88 percent) of the nation's 57 dental schools in continuing operation. Forty-two schools reported discrete GDEA at the time of the study. Seven of these schools had a formal division or department of geriatric dentistry. The survey indicated that educational qualification of GDEA coordinators had risen significantly in recent years. The mean number of didactic hours (29) of instruction in geriatric education available to students has also risen. Curriculum time and financial considerations were the primary obstacles to expansion of clinical geriatric activities. With the current economic constraints in dental education, present GDEA levels are unlikely to expand in the foreseeable future, unless governmental or private agencies recognize the importance of developing GDEA and increase their support.


Central infusion of aliskiren prevents sympathetic hyperactivity and hypertension in Dahl salt-sensitive rats on high salt intake.
Central infusion of an angiotensin type 1 (AT(1)) receptor blocker prevents sympathetic hyperactivity and hypertension in Dahl salt-sensitive (S) rats on high salt. In the present study, we examined whether central infusion of a direct renin inhibitor exerts similar effects. Intracerebroventricular infusion of aliskiren at the rate of 0.05 mg/day markedly inhibited the increase in ANG II levels in the cerebrospinal fluid and in blood pressure (BP) caused by intracerebroventricular infusion of rat renin. In Dahl S rats on high salt, intracerebroventricular infusion of aliskiren at 0.05 and 0.25 mg/day for 2 wk similarly decreased resting BP in Dahl S rats on high salt. In other groups of Dahl S rats, high salt intake for 2 wk increased resting BP by ∼25 mmHg, enhanced pressor and sympathoexcitatory responses to air-stress, and desensitized arterial baroreflex function. All of these effects were largely prevented by intracerebroventricular infusion of aliskiren at 0.05 mg/day. Aliskiren had no effects in rats on regular salt. Neither high salt nor aliskiren affected hypothalamic ANG II content. These results indicate that intracerebroventricular infusions of aliskiren and an AT(1) receptor blocker are similarly effective in preventing salt-induced sympathetic hyperactivity and hypertension in Dahl S rats, suggesting that renin in the brain plays an essential role in the salt-induced hypertension. The absence of an obvious increase in hypothalamic ANG II by high salt, or decrease in ANG II by aliskiren, suggests that tissue levels do not reflect renin-dependent ANG II production in sympathoexcitatory angiotensinergic neurons.


[Complementary tests: conventional and Holter electrocardiography].
Arterial hypertension can cause left ventricular hypertrophy and consequent hypertensive cardiopathy, a condition that is associated with the development of heart failure, arrhythmia and ischemia. Electrocardiography is the oldest and simplest method for diagnosing left ventricular hypertrophy: nevertheless, though established electrocardiographic criteria are highly specific, their sensitivity is low, far below that of echocardiography. There are, however, new electrocardiographic criteria with improved sensitivity; moreover, the presence of electrocardiographic signs of hypertrophy in a hypertensive patient makes a diagnosis of hypertrophy highly probable. The Holter monitor has many applications in the assessment of hypertensive patients. It identifies auricular and ventricular arrhythmias in terms of anatomical patterns, and can be used to evaluate drug response and detect the presence of silent ischemia and sudden death. In conclusion, electrocardiography is an easily accessible tool and a highly useful one in the management of hypertensive patients, in spite of its limitations.


Brachial artery stiffness estimation using ARTSENS.
Central and peripheral arteries stiffening prominently affect hemodynamics thus increasing the risk of coronary heart disease, chronic kidney disease and end stage renal disease. There are several commercially available non-invasive measurement technologies for the evaluation of stiffness that are expensive, demand dedicated expertise and fall short for mass screening. Considering this, we have developed ARTSENS®, a highly compact and portable image-free ultrasound device for evaluation of arterial stiffness. The capability of the device to perform accurate measurements of carotid artery stiffness has been validated through extensive in-vivo studies. In this paper we demonstrate the feasibility of using ARTSENS® for measuring brachial artery stiffness. An inter-operator repeatability study was done based on in-vivo experiments on 9 young healthy subjects. The study included measurement of distension, end diastolic lumen diameter, arterial compliance and stiffness index performed both on carotid artery and brachial artery by two operators successively. The degree of agreement between the measurements made by operators has been investigated based on Bland-Altman plots and paired t-test. The measurements were populated within the limits of agreement. No statistically significant difference (p-values from paired t-test for end-diastolic diameter, distension, stiffness index, arterial compliance were 0.36, 0.24, 0.47 and 0.11 respectively) was seen for the brachial artery measurements performed by the two operators. The correlation between the measurement made by the operators was highly significant (r=0.86, p-value=0.003).


Simulation for multiwavelength large-aperture all-silicon metalenses in long-wave infrared.
Long-wave infrared imaging systems are widely used in the field of environmental monitoring and imaging guidance. As the core components, the long-wave infrared lenses suffer the conditions of less available materials, difficult processing, large volume and mass. Metalens composed of sub-wavelength structures is one of the most potential candidates to achieve a lightweight and planar optical imaging systems. Meanwhile, it is essential to obtain large-aperture infrared lenses with high power and high resolution. However, it is difficult to use the finite-difference time-domain method to simulate a large-aperture metalens with the diameter of 201 mm due to the large amount of computational memory and computational time required. Here, to solve the mentioned problem, we firstly propose a simulation method for designing a large-aperture metalens, which combines the finite-difference time-domain algorithm and diffraction integration. The finite-difference time-domain algorithm is used to simulate the meta-atom's transmitted complex amplitude and the one-dimensional simplification of the diffraction integral is to calculate the focused field distributions of the designed metalens. Furthermore, the meta-atom spatial multiplexing is applied to design the all-silicon metalenses with the aperture of 201 mm to realize dual-wavelength (10 and 11μm) achromatic focusing, super anomalous dispersion focusing and super normal dispersion focusing. The designed metalenses are numerically confirmed, which reveal the feasibility of all-silicon sub-wavelength structures to accomplish the multiwavelength dispersion control. The designed all-silicon metalenses have the advantage of lightweight and compact. The proposed method is effective for the development of large-aperture imaging systems in the long-wave infrared.


The anti-diabetic and antioxidant effects of a combination of Commiphora mukul, Commiphora myrrha and Terminalia chebula in diabetic rats.
Effects of Commiphora mukul and Commiphora myrrha ethanolic extracts and Terminalia chebula hydro-ethanolic extract combination were evaluated in streptozotocin (STZ)-induced diabetic rats. Male Wistar rats (n=48) were randomly assigned into: control; diabetic; diabetic+metformin (300 mg/kg); diabetic+dose 1 of herbal combination (438 mg/kg of C. mukul+214 mg/kg of C. myrrha+857 mg/kg of T. chebula); diabetic+dose 2 (642 mg/kg of C. mukul+214 mg/kg of C. myrrha+642 mg/kg of T. chebula); and diabetic+dose 3 (857 mg/kg of C. mukul+438 mg/kg of C. myrrha+1714 mg/kg t of T. chebula). All treatments were given orally by gavage. Diabetes was induced by STZ (60 mg/kg, i.p.). At the end of study (day 28), blood glucose, insulin and lipid profile; as well as hepatic malondialdehyde (MDA) and thiol content, and superoxide dismutase (SOD) and catalase (CAT) activities were determined. In diabetic rats, plasma glucose, triglycerides (TG), total cholesterol (TC), and LDL-C, as well as hepatic MDA levels were elevated but plasma HDL-C and insulin, and hepatic thiol content and SOD and CAT activities were reduced compared to control (p<0.01-p<0.001). In diabetic+dose 3, plasma TC, TG, and LDL-C and hepatic MDA level decreased (p<0.001), while plasma HDL-C and insulin, and hepatic thiol content, and SOD and CAT activities increased compared to diabetic (p<0.01-p<0.001). Treatment with dose 1 and 2 improved such abnormalities in diabetic rats except for insulin level (p<0.05-p<0.001). The herbal combination effects were comparable to those of metformin. Metformin did not significantly change serum insulin and HDL-C levels, and hepatic SOD activity; however, serum levels of TC, TG, and LDL-C, as well as hepatic MDA levels, thiol content and CAT activity were improved compared to diabetic (p<0.05-p<0.001). These results indicate that this herbal combination acts as an anti-diabetic, antioxidant and hypolipidemic agent and it may be suggested as a beneficial remedy for diabetic patients.


Intravenous immunoglobulin immunotherapy for coronavirus disease-19 (COVID-19).
Intravenous immunoglobulin (IVIG), a pooled normal IgG from several thousand healthy donors and one of the commonly used immunotherapeutic molecules for the management of autoimmune and inflammatory diseases, has been explored for the treatment of coronavirus disease-19 (COVID-19). Although placebo-controlled, double-blind randomised clinical trials are lacking, current data from either retrospective, case series or open-label randomised controlled trials provide an indicator that IVIG immunotherapy could benefit severe and critically ill COVID-19 patients. See alsoShao et al.


Lysophosphatidic acid induces focal adhesion assembly through Rho/Rho-associated kinase pathway in human ovarian cancer cells.
The level of lysophosphatidic acid (LPA) is elevated in patients with ovarian cancer, and LPA has been reported to have a pivotal role in cancer dissemination. In the current study, the effect of LPA on the motility of ovarian cancer cells was investigated. We analyzed the effects of LPA on the migration activity, the focal adhesion formation, and the tyrosine phosphorylation of focal adhesion proteins in human ovarian cancer cell lines Caov-3 and OVCAR-3. Inhibitors of the small GTPase Rho, one of its downstream effectors (Rho-associated kinase (ROCK)), myosin light chain kinase (MLCK), and myosin light chain (MLC) phosphatase were used to examine the mechanism of LPA-induced cellular effects. LPA enhanced the migration of ovarian cancer cells and facilitated their invasion. Rho and ROCK played essential roles in the migratory process, as evidenced by the inhibition of migration and focal adhesion formation of cancer cells by Clostridium botulinum C3 exoenzyme (C3), an inhibitor of Rho, or Y-27632, an inhibitor of ROCK. LPA also evoked the formation of focal adhesions and tyrosine phosphorylation of focal adhesion kinase and paxillin, all of which were inhibited by C3 or Y-27632. These results suggest that LPA induced the migration of ovarian cancer cells, at least in part, through accelerated formation of focal adhesions mediated by Rho/ROCK-induced actomyosin contractility. This study may provide the basis for new therapies to control the metastasis of ovarian cancer.


The effects of hypertonic saline solution (7.5%) on coagulation and fibrinolysis: an in vitro assessment using thromboelastography.
We studied the effects of hypertonic (7.5%) and normal saline on coagulation and fibrinolysis in an in vitro model using thromboelastography of human whole blood. Reaction times increased and alpha angles decreased with hypertonic saline replacement at 7.5% blood volume compared with similar dilution with normal saline. At 10% blood volume replacement with hypertonic saline, reaction and coagulation times were significantly increased and alpha angles were decreased. Clot lysis at 30 min was also significantly reduced. We conclude that 7.5% hypertonic saline solution has anticoagulant effects if it replaces 7.5% or more of blood volume.


The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions.
Ferritin, a spherically shaped protein complex, is responsible for iron storage in bacteria, plants, animals, and humans. Various ferritin iron core compositions in organisms are associated with specific living requirements, health state, and different biochemical roles of ferritin isomers. Magnetoferritin, a synthetic ferritin derivative, serves as an artificial model system of unusual iron phase structures found in humans. We present the results of a complex structural study of magnetoferritins prepared by controlled in vitro synthesis. Using various complementary methods, it was observed that manipulation of the synthesis technology can improve the physicochemical parameters of the system, which is useful in applications. Thus, a higher synthesis temperature leads to an increase in magnetization due to the formation of the magnetite phase. An increase in the iron loading factor has a more pronounced impact on the protein shell structure in comparison with the pH of the aqueous medium. On the other hand, a higher loading factor at physiological temperature enhances the formation of an amorphous phase instead of magnetite crystallization. It was confirmed that the iron-overloading effect alone (observed during pathological events) cannot contribute to the formation of magnetite.


A Digital Feature Recognition Technology Used in Ballet Training Action Correction.
In order to improve the effect of ballet training, this paper combines digital technology to improve the motion recognition process, analyzes the imaging process, analyzes the system process combined with the human node model, and combines digital feature recognition technology to construct a ballet training motion correction system. Moreover, this paper inputs the ballet training action recognition results into the system and compares the standard actions to judge the rationality of the actions and builds a ballet training action correction system based on digital feature recognition. In addition, this paper designs experiments to conduct ballet training movements in the system effect evaluation. The experimental research verifies that the digital feature recognition technology proposed in this paper can play an important role in ballet movement recognition and has a good action correction effect.


[Effects of electroacupuncture on BNDF/mTORC1 signaling pathway and synaptic plasticity in prefrontal cortex of rats exposed to chronic unpredictable mild stress].
To investigate the effects of electroacupuncture (EA) on the expression of related proteins in the brain-derived neurotrophic factor (BDNF)/mammalian target of rapamycin complex 1 (mTORC1) signaling pathway and synapse-associated proteins and the density of dendrite spines in the prefrontal cortex (PFC) of depression model rats, and to reveal the underlying mechanism by which EA regulates the synaptic plasticity to improve depressive symptoms. Thirty-six healthy male Sprague-Dawley (SD) rats were randomly divided into normal group, model group, EA group, and scopolamine (SCOP) group, with 9 in each group. The depression model was established by exposing rats to chronic unpredictable mild stress (CUMS) combined with isolated feeding. Rats in the EA group were treated with EA (2 Hz/100 Hz, 1-1.2 mA) at "Baihui" (GV20), "Yintang" (EX-HN3), "Hegu" (LI4), and "Taichong" (LR3), 20 min each time, once per day, for 14 d, while those in the SCOP group treated with intraperitoneal injection of 25 μg/kg SCOP, once every 16 h, for 14 d. The sucrose preference and feeding latency of rats in each group were observed in the sucrose preference test (SPT) and novelty-suppressed feeding test. The expression levels of proteins in the BDNF/mTORC1 signaling pathway and synapse-associated proteins PSD95, Synapsin Ⅰ, and GluR1 were assayed by Western blot. Golgi-Cox staining was conducted for exploring the total density of dendritic spines on the apical dendrites of layer Ⅴ pyramidal neurons in PFC as well as the densities of mature, immature, and filopodial-like dendritic spines. Compared with the normal group, the model group exhibited significantly decreased sucrose preference (P<0.001), prolonged feeding latency (P<0.001), down-regulated BDNF, mTORC1, phosphorylated mTORC1 (p-mTORC1), PSD95, Synapsin Ⅰ, and GluR1 expression (P<0.001,P<0.01), and diminished total, mature, and immature spine dendritic densities (P<0.001). Compared with the model group, both EA and SCOP remarkably increased the sucrose preference (P<0.001), shortened the feeding latency (P<0.001), up-regulated the BDNF, mTORC1, p-mTORC1, PSD95, Synapsin Ⅰ, and GluR1 expression in PFC(P<0.05,P<0.01,P<0.001), and elevated the total and immature spine dendritic densities (P<0.001,P<0.01). The density of filopodial-like dendritic spine in the EA group was obviously enhanced (P<0.01), whereas the mature dendritic spine density in the SCOP group rose sharply (P<0.001). However, there were no significant differences between the EA group and SCOP group (P>0.05). EA alleviates the depressive symptoms of CUMS model rats possibly by up-regulating the expression of proteins in the BDNF/mTORC1 signaling pathway and synapse-asso-ciated proteins PSD95, Synapsin Ⅰ, and GluR1, increasing the dendritic spine density, and enhancing the synaptic plasticity in PFC.


Expression of fibronectin-binding protein FbpA modulates adhesion in Streptococcus gordonii.
Fibronectin binding is considered to be an important virulence factor in streptococcal infections. Adhesion of the oral bacterium Streptococcus gordonii to immobilized forms of fibronectin is mediated, in part, by a high molecular mass wall-anchored protein designated CshA. In this study, a second fibronectin-binding protein of S. gordonii is described that has been designated as FbpA (62.7 kDa). This protein, which is encoded by a gene located immediately downstream of the cshA gene, shows 85 and 81% identity to the fibronectin-binding proteins PavA, of Streptococcus pneumoniae, and FBP54, of Streptococcus pyogenes, respectively. Purified recombinant FbpA bound to immobilized human fibronectin in a dose-dependant manner, and isogenic mutants in which the fbpA gene was inactivated were impaired in their binding to fibronectin. This effect was apparent only for cells in the exponential phase of growth, and was associated with reduced surface hydrophobicity and the surface expression of CshA. Cells in the stationary phase of growth were unaffected in their ability to bind to fibronectin. By utilizing gene promoter fusions with cat (encoding chloramphenicol O-acetyltransferase), it was demonstrated that cshA expression was down-regulated during the exponential phase of growth in the fbpA mutant. Expression of fbpA, but not cshA, was sensitive to atmospheric O2 levels, and was found to be up-regulated in the presence of elevated O2 levels. The results suggest that FbpA plays a regulatory role in the modulation of CshA expression and, thus, affects the adhesion of S. gordonii to fibronectin.


Benefits of family and social relationships for Thai parents living with HIV.
Family and social relationships are important structural supports in Thailand that are likely to influence the health and mental health of persons living with HIV (PLH). Structural equation modeling examined these relationships among 409 PLH in two communities in Thailand. Latent variables were constructed for most outcomes and mediators, with adherence to antiretroviral (ARV) therapy, depression, and disclosure represented by single-item indicators. All models controlled for gender, age, and education. Disclosure was significantly and positively associated with ARV adherence, and to both family functioning and social support. Family functioning and social support were significantly related to the PLH's self-perceptions of health and mental health, as well as being significantly correlated with each other. Better family functioning was significantly associated with better quality of life, better perceived health, fewer symptoms of depression, and greater ARV adherence. Social support was significantly associated with better quality of life and fewer depressive symptoms. These results highlight the important role that an organized and structured family life and social support network can play in encouraging better health outcomes among PLH.


Auditory evoked potentials and multiple sclerosis.
Multiple sclerosis (MS) is an inflammatory, demyelinating disease that can affect several areas of the central nervous system. Damage along the auditory pathway can alter its integrity significantly. Therefore, it is important to investigate the auditory pathway, from the brainstem to the cortex, in individuals with MS. The aim of this study was to characterize auditory evoked potentials in adults with MS of the remittent-recurrent type. The study comprised 25 individuals with MS, between 25 and 55 years, and 25 age- and gender-matched healthy controls (research and control groups). Subjects underwent audiological and electrophysiological evaluations. Statistically significant differences were observed between the groups regarding the results of the auditory brainstem response and the latency of the Na and P300 waves. Individuals with MS present abnormalities in auditory evoked potentials indicating dysfunction of different regions of the central auditory nervous system.


When Does Diversity Help Generalization in Classification Ensembles?
Ensembles, as a widely used and effective technique in the machine learning community, succeed within a key element-"diversity." The relationship between diversity and generalization, unfortunately, is not entirely understood and remains an open research issue. To reveal the effect of diversity on the generalization of classification ensembles, we investigate three issues on diversity, that is, the measurement of diversity, the relationship between the proposed diversity and the generalization error, and the utilization of this relationship for ensemble pruning. In the diversity measurement, we measure diversity by error decomposition inspired by regression ensembles, which decompose the error of classification ensembles into accuracy and diversity. Then, we formulate the relationship between the measured diversity and ensemble performance through the theorem of margin and generalization and observe that the generalization error is reduced effectively only when the measured diversity is increased in a few specific ranges, while in other ranges, larger diversity is less beneficial to increasing the generalization of an ensemble. Besides, we propose two pruning methods based on diversity management to utilize this relationship, which could increase diversity appropriately and shrink the size of the ensemble without much-decreasing performance. The empirical results validate the reasonableness of the proposed relationship between diversity and ensemble generalization error and the effectiveness of the proposed pruning methods.


[Internal migration in Italy: an overview of studies in the early 1990s].
The author examines "the content of the studies mentioned in the bibliography in order to [investigate]...both the most recent general tendencies of short and long distance migrations [in Italy]. Economic variables appear to have lost a good deal of their explanatory capacity on behalf of causal factors linked with the ¿life cycle'. Also mentioned are the main conceptual categories used in order to define the consequences of migrations on the spatial distribution of populations: so-called ¿atomistic' settlement models, based on administrative units, are now in competition with the growing importance of ¿functional' models, based on pull areas." (SUMMARY IN ENG AND FRE)


Promoting Whole Health in the Dental Setting: Steps Toward an Integrated Interprofessional Clinical Learning Environment Involving Pharmacy, Social Work, and Nursing.
Dental settings have not traditionally functioned as access points to the health care system, however they can serve patients who may not otherwise seek routine health care. Millions of Americans annually visit either a dental or primary care provider, but not always both as recommended, even though multiple health co-morbidities can manifest in and impact oral health. Offering multidisciplinary health services in a dental setting has potential to reach unserved populations. Innovative partnerships between schools of dentistry, pharmacy, social work, and nursing were designed to promote integrated service delivery in the emerging workforce and the purposeful inclusion of oral health in integrated care settings. Oral complications of systemic disease and systemic complications of oral disease impose significant burdens on populations and the public health infrastructure in terms of economic cost, disability, and mortality. Exacerbated by the lack of integrated services, intersecting social, economic, and health issues perpetuate disparities and negative health outcomes. Care is often focused on reactive rather than preventive measures therefore addressing only the acute issue instead of the underlying, causative problem(s). We describe steps for integrated, whole-health services and lessons learned for other academic health institutions and interprofessional settings considering integrated clinical models.


Water extract of ashwagandha leaves limits proliferation and migration, and induces differentiation in glioma cells.
Root extracts of Withania somnifera (Ashwagandha) are commonly used as a remedy for a variety of ailments and a general tonic for overall health and longevity in the Indian traditional medicine system, Ayurveda. We undertook a study to investigate the anti-proliferative and differentiation-inducing activities in the water extract of Ashwagandha leaves (ASH-WEX) by examining in glioma cells. Preliminary detection for phytochemicals was performed by thin-layer chromatography. Cytotoxicity was determined using trypan blue and MTT assays. Expression level of an hsp70 family protein (mortalin), glial cell differentiation marker [glial fibrillary acidic protein (GFAP)] and neural cell adhesion molecule (NCAM) were analyzed by immunocytochemistry and immunoblotting. Anti-migratory assay was also done using wound-scratch assay. Expression levels of mortalin, GFAP and NCAM showed changes, subsequent to the treatment with ASH-WEX. The data support the existence of anti-proliferative, differentiation-inducing and anti-migratory/anti-metastasis activities in ASH-WEX that could be used as potentially safe and complimentary therapy for glioma.


Noninvasive monitoring of CO2 during polysomnography: a review of the recent literature.
Sleep-related breathing disorders are complex conditions that require the integration of clinical and sleep laboratory findings to support a diagnosis. Analysis of carbon dioxide (CO2) levels during sleep provides important additional information to the clinician that is not obtained from other polysomnographic indices, and that may have a direct impact on both diagnosis and patient mortality. Although arterial blood gas (ABG) is considered the gold standard for assessing PaCO2 levels, there are numerous drawbacks. Noninvasive methods for PaCO2 estimation include end-tidal and transcutaneous monitoring, which allow for continuous monitoring of trends. Review of the recent literature suggests that transcutaneous methods correlate strongly with PaCO2 levels and can provide an accurate surrogate in replacement of ABGs. End-tidal methods provide breath to breath information that can be used to assess hypoventilation; however, they have more variability, especially in patients with increased dead space and small tidal volumes. To date, however, there are limited studies investigating noninvasive CO2 monitoring during sleep. Given the benefits of CO2 monitoring and the importance of assessing for hypercapnia, noninvasive continuous CO2 monitoring should be considered for all patients undergoing polysomnography.


[Effect of pressure-induced ischemia on oxygen supply in the retinal capillary area. Results of a mathematical model].
This paper attempts to obtain a better understanding of the oxygen supply to tissue cells of the optic nerve head by describing all phases of the release, diffusion and consumption process by means of a mathematical model. As a result the oxygen partial pressure reduction induced by intraocular pressure elevation--as it occurs in glaucoma--seems to be mainly influenced by a pressure-induced lowering of the average blood velocity in the retinal capillaries. A reduction of this velocity to a fourth of its normal value even makes the oxygen partial pressure at the venous capillary wall drop below the critical level necessary for maintaining cell activity. Furthermore our calculations allow us to conclude that an involvement of the retinal capillary network supplied by the central retinal artery should not be neglected in glaucoma.


The "hot cross bun sign" in patients with autoimmune cerebellar ataxia: A case report and literature review.
The "hot cross bun sign" (HCBs) on magnetic resonance imaging (MRI) has been initially considered specific for multiple system atrophy with cerebellar features. However, a number of other conditions have since been described, which may be associated with this imaging sign. We herein describe a patient with anti-Ri and paraneoplastic cerebellar ataxia, and review the association of the HCBs on imaging with various neurological autoimmune conditions. We report a 40-year-old woman with anti-Ri-associated paraneoplastic neurological syndrome and breast carcinoma, in whom brain MRI revealed the HCBs late in the disease course. We also reviewed similar cases reported in the literature. The patient presented with cerebellar ataxia, polyneuropathy, and pyramidal signs. Although brain MRI was initially unremarkable, the HCBs and T2-weighted hyperintensity of the bilateral middle cerebellar peduncles were observed at later follow-up. Anti-Ri was detected in the serum and cerebrospinal fluid. Breast adenocarcinoma was confirmed via an axillary lymph node biopsy. Her symptoms partially resolved after the first corticosteroid pulse. However, subsequent immunotherapy and tumor treatments were ineffective. Four autoimmune cerebellar ataxia cases with the HCBs (two paraneoplastic and two non-paraneoplastic) were identified in the literature. The HCBs can be associated with paraneoplastic and non-paraneoplastic cerebellar ataxia, which may reflect neurodegeneration secondary to autoimmune injury. Thus, the HCBs should not be considered a contraindication for autoimmune cerebellar syndrome.


Human Genetics Society of Australasia Position Statement: Online DNA Testing.
Increasingly, consumers have been able to seek DNA testing online to explore their personal genetic information. This increased access to a range of genomic tests has raised concerns among health professionals tasked with providing guidance and support to patients requiring genetic/genomic testing. Individuals will seek genomic testing for a range of purposes; equally, the medical marketplace offers a range of different test types. The Human Genetics Society of Australasia (HGSA) published their first statement on Direct to Consumer Genetic Testing (2012 PS02). This is a revised statement, which considers developments in the field of online DNA testing, including rapid technological changes, diversity of applications and decreasing costs of testing. It draws from the first empirical nationwide study (Genioz - Genomics: National Insights of Australians) and insights from consumers with experience of this technology. The rapid adoption of these tests and the broad range of potential consequences have informed perspectives within this statement. It is the position of the HGSA that both individuals/consumers and health care professionals/providers should be supported to make informed choices about online DNA testing. This means adequate and ongoing education and resources should be available for individuals/consumers and health care professionals/providers before, during and after testing. Health care professionals/providers should be appropriately trained, have relevant experience and should be able to demonstrate (or provide evidence of) a current certification in their field of practice. This statement was ratified at the 2018 HGSA Council Meeting and was recently reviewed in 2019 for consistency with other HGSA position statements.


[Tinnitus: algorithm of diagnostics and clinical management].
Hearing of sound, or tinnitus, can be a symptom of different diseases. The differential diagnosis should be based on the identification of subgroups with confirmed causes of the disease. Subjective and objective tinnitus groups should be isolated. Objective tinnitus can be vascular or muscular. In making a diagnosis of tinnitus, it is important to know its characteristics, laterality, circumstances of onset, duration, comorbidity with other symptoms: headache, hearing decline, dizziness, depression, etc. Urgent diagnostic and treatment measures are needed after the identification of 'red flags': acute pulsatile tinnitus, in particular after the brain injury, combination of tinnitus with acute hearing loss and depression.


β-Stereoselective phosphorylations applied to the synthesis of ADP- and polyprenyl-β-mannopyranosides.
An efficient and convenient synthetic route to glycosyl 1-β-phosphates has been developed using diallyl chlorophosphate as a phosphorylating agent with 4-N,N-dimethylaminopyridine under mild conditions. Diallyl-glycosyl 1-β-phosphate triesters of D-manno, L-glycero-D-manno-hepto-, D-gluco-, D-galacto-, and L-fuco-pyranose as well as lactose have been obtained by this strategy in good yields and excellent β-selectivities. Furthermore, the diallyl 6-azido-mannosyl 1-β-phosphate 2 was deprotected under mild conditions and converted into potentially clickable analogues of β-mannosyl phosphoisoprenoids I and ADP-heptose II.


Cytochrome b gene structure and consequences for resistance to Qo inhibitor fungicides in plant pathogens.
The cytochrome b (cyt b) gene structure was characterized for different agronomically important plant pathogens, such as Puccinia recondita f sp tritici (Erikss) CO Johnston, P graminis f sp tritici Erikss and Hennings, P striiformis f sp tritici Erikss, P coronata f sp avenae P Syd & Syd, P hordei GH Otth, P recondita f sp secalis Roberge, P sorghi Schwein, P horiana Henn, Uromyces appendiculatus (Pers) Unger, Phakopsora pachyrhizi Syd & P Syd, Hemileia vastatrix Berk & Broome, Alternaria solani Sorauer, A alternata (Fr) Keissl and Plasmopara viticola (Berk & Curt) Berlese & de Toni. The sequenced fragment included the two hot spot regions in which mutations conferring resistance to QoI fungicides may occur. The cyt b gene structure of these pathogens was compared with that of other species from public databases, including the strobilurin-producing fungus Mycena galopoda (Pers) P Kumm, Saccharomyces cerevisiae Meyer ex Hansen, Venturia inaequalis (Cooke) Winter and Mycosphaerella fijiensis Morelet. In all rust species, as well as in A solani, resistance to QoI fungicides caused by the mutation G143A has never been reported. A type I intron was observed directly after the codon for glycine at position 143 in these species. This intron was absent in pathogens such as A alternata, Blumeria graminis (DC) Speer, Pyricularia grisea Sacc, Mycosphaerella graminicola (Fuckel) J Schröt, M fijiensis, V inaequalis and P viticola, in which resistance to QoI fungicides has occurred and the glycine is replaced by alanine at position 143 in the resistant genotype. The present authors predict that a nucleotide substitution in codon 143 would prevent splicing of the intron, leading to a deficient cytochrome b, which is lethal. As a consequence, the evolution of resistance to QoI fungicides based on G143A is not likely to evolve in pathogens carrying an intron directly after this codon.


Depletion and Dysfunction of Dendritic Cells: Understanding SARS-CoV-2 Infection.
Uncontrolled severe acute respiratory syndrome-coronavirus (SARS-CoV)-2 infection is closely related to disorders of the innate immune and delayed adaptive immune systems. Dendritic cells (DCs) "bridge" innate immunity and adaptive immunity. DCs have important roles in defending against SARS-CoV-2 infection. In this review, we summarize the latest research concerning the role of DCs in SARS-CoV-2 infection. We focus on the complex interplay between DCs and SARS-CoV-2: pyroptosis-induced activation; activation of the renin-angiotensin-aldosterone system; and activation of dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin. We also discuss the decline in DC number, the impaired antigen-presentation capability, and the reduced production of type-I interferon of DCs in severe SARS-CoV-2 infection. In addition, we discuss the potential mechanisms for pathological activation of DCs to understand the pattern of SARS-CoV-2 infection. Lastly, we provide a brief overview of novel vaccination and immunotherapy strategies based on DC targeting to overcome SARS-CoV-2 infection.


Cure of antimony-unresponsive Indian visceral leishmaniasis with amphotericin B lipid complex.
Twenty-one Indian patients with visceral leishmaniasis who did not respond to or relapsed after 28-60 days of pentavalent antimony therapy were treated with amphotericin B lipid complex (ABLC). Five infusions (3 mg/kg each) given every second day over 9 days (total dose, 15 mg/kg) resulted in a 100% apparent cure response. In 4 other patients who had not responded to antimony, apparent cure was also induced by ABLC given 3 mg/kg a day 5 consecutive days (total dose, 15 mg/kg). Fever and chills developed routinely during the initial 2-h infusions; these reactions were tolerated and diminished with successive infusions. Six months after treatment, all 25 patients were healthy, had parasite-free bone marrow aspirates, and were considered cured. ABLC is effective short-course therapy for kala-azar patients who do not respond to conventional antimony treatment.


Requirement of both tyrosine residues 1330 and 1337 in the C-terminal tail of the RON receptor tyrosine kinase for epithelial cell scattering and migration.
RON is a receptor tyrosine kinase that mediates cell scattering, migration, and tubular formation. This study focused on the function of two tyrosines, Y1330 and Y1337, in the C-terminus of RON in regulating epithelial cell scattering and migration. Substitution of both tyrosine residues with phenylalanine causes complete loss of cell scattering and migration in kidney 293 cells. In contrast, single mutation of either tyrosine residue has no effect. We found that mutation at Y1330 or Y1337 alone does not significantly affect the association of RON with PI-3 kinase, whereas a double mutation abolishes the recruitment of substrates. RON-mediated cell migration was inhibited by PI-3 kinase inhibitor wortmannin. This effect was also achieved by a dominant inhibitory p85 of PI-3 kinase. We conclude that Y1330 and Y1337 are required for RON-mediated cell motility. By associating with PI-3 kinase, the Y1330-Y1337 docking site plays a critical role in transducing motile signals of RON.


Improved Retention Performance in Graphene-Ferroelectric Memory Device Through Mitigation of the Surface Roughness of the Ferroelectric Layer.
A ferroelectric-gated graphene field-effect transistor was fabricated with a bottom-gate structure. A ferroelectric gate dielectric was formed using the spin-coating method. Two samples were made, one with a single coating and the other with a double coating. It was observed that the data retention times of the two samples were significantly different. When comparing the surface roughness, the surface of the thin film produced by the double coating was much flatter. Based on the observation of the surface morphology, an interfacial layer composed of air was deduced as the origin of both the depolarization and short retention time. That is, when fabricating the graphene-ferroelectric memory device, the importance of the interface treatment could be confirmed. Based on these results, it is expected that a much more reliable device can be realized through surface engineering via a graphene-ferroelectric device process.


Altered expression of phosphatase of regenerating liver gene family in non-small cell lung cancer.
Protein tyrosine phophatases (PTPs) are implicated in the tumorigenesis and metastasis of human cancer. The phosphatase of regenerating liver (PRL) gene family, a subgroup of PTPs is also linked to these processes. In many solid cancers, high levels of PRL-3 expression are related with metastasis and poor prognosis. However, the expression patterns of PRL-1 and -2 have not been explored in lung cancer yet. Thus, we investigated the expression levels of PRL-1, -2 and -3 in the tissues of primary lung cancer patients. The protein expression levels of PRL-2, but not PRL-1 and -3 were increased in cancer tissues. However, there was no correlation between mRNA and protein expression levels of the PRLs. Reporter assays showed that PRLs suppressed the activity of the p21 promoter but promoted AP-1 activity. Furthermore, transfection of PRLs showed significantly increased cell proliferation. Therefore, these results suggest that PRL-2 plays an important role in lung cancer and can be a biomarker of lung cancer, substituting for the function of other PRLs.


Linezolid activity against disseminated Listeria monocytogenes meningitis and central nervous system abscesses: focus on early drug myelotoxicity.
A very infrequent report of Listeria monocytogenes meningitis and multiple subtentorial abscesses (cerebellar, bulbar, and pontine in location), occurred in an immunocompetent man, and is described and discussed on the ground of the most recent literature evidences regarding the frequency, presentation, and outcome of this disease. The possibility to resort to combination linezolid therapy in patients who appear to be not responsive to first-line treatment of cerebral abscess of definite origin, and the appearance of anemia as an early untoward event of this novel oxazolidinone antibiotic, are also debated.


Investor attention and cryptocurrency: Evidence from the Bitcoin market.
This paper adds to the growing literature of cryptocurrency and behavioral finance. Specifically, we investigate the relationships between the novel investor attention and financial characteristics of Bitcoin, i.e., return and realized volatility, which are the two most important characteristics of one certain asset. Our empirical results show supports in the behavior finance area and argue that investor attention is the granger cause to changes in Bitcoin market both in return and realized volatility. Moreover, we make in-depth investigations by exploring the linear and non-linear connections of investor attention on Bitcoin. The results indeed demonstrate that investor attention shows sophisticated impacts on return and realized volatility of Bitcoin. Furthermore, we conduct one basic and several long horizons out-of-sample forecasts to explore the predictive ability of investor attention. The results show that compared with the traditional historical average benchmark model in forecasting technologies, investor attention improves prediction accuracy in Bitcoin return. Finally, we build economic portfolios based on investor attention and argue that investor attention can further generate significant economic values. To sum up, investor attention is a non-negligible pricing factor for Bitcoin asset.


Microhardness evaluation of root dentin after using resin sealer solvents.
Background. This study aimed to assess the effects of methyl ethyl ketone (MEK) and ethyl acetate (EA) on dentin microhardness, used as resin sealer solvents. Methods. Eighty halves of single-rooted teeth were randomly divided into four groups to apply MEK, EA, chloroform, or saline solution. Vickers hardness values were measured for three root levels before and after the direct application of solvents for 5 and 15 minutes or a 1-minute application with ultrasonic agitation. The results were analyzed using repeated-measures ANOVA, and adjustments were made for comparisons with Bonferroni tests. Results. The dentin microhardness decreased in all the solvent groups (P < 0.05). The changes in microhardness increased with prolonged exposure times, except for the saline solution group. Chloroform exhibited the most significant decrease in value. Furthermore, ultrasonic agitation elicited a more substantial decline in values. Conclusion. MEK and EA might be preferred over chloroform as a solvent for resin sealers because they offer an attenuated decrease in dentin microhardness and do not have gutta-percha-dissolving properties.


Aortic remodelling during hemogenesis: is the chicken paradigm unique?
Since the era of the ancient Egyptians and Greeks, the avian embryo has been a subject of intense interest to visualize the first steps of development. It has served as a pioneer model to scrutinize the question of hematopoietic development from the beginning of the 20th century. It's large size and easy accessibility have permitted the development of techniques dedicated to following the origins and fates of different cell populations. Here, we shall review how the avian model has brought major contributions to our understanding of the development of the hematopoietic system in the past four decades and how these discoveries have influenced our knowledge of mammalian hematopoietic development. The discovery of an intra-embryonic source of hematopoietic cells and the developmental link between endothelial cells and hematopoietic cells will be presented. We shall then point to the pivotal role of the somite in the construction of the aorta and hematopoietic production and demonstrate how two somitic compartments cooperate to construct the definitive aorta. We shall finish by showing how fate-mapping experiments have allowed the identification of the tissue which gives rise to the sub-aortic mesenchyme. Taken together, this review aims to give an overview of how and to what extent the avian embryo has contributed to our knowledge of developmental hematopoiesis.


Charcot spine treated using a single staged posterolateral costotransversectomy approach in a patient with traumatic spinal cord injury.
Charcot spine is a progressive and destructive process that affects the vertebral bodies, intervertebral discs, and posterior facets. It is the result from repetitive microtrauma in patients who have decreased joint protective mechanisms due to loss of deep pain and proprioceptive sensation, typically because of spinal cord injury. The objective of the study is to report an unusual case of Charcot spine, as a late complication of traumatic spinal cord injury, treated by a circumferential arthrodesis performed with a single staged posterolateral costotransversectomy approach.


Systematic Review and Meta-Analysis of the Association Between Non-Steroidal Anti-Inflammatory Drugs and Operative Bleeding in the Perioperative Period.
It is increasingly recognized that non-opioid analgesia is an important analgesia in the perioperative period. Specifically, NSAIDs (nonsteroidal anti-inflammatory drugs) have been touted as an adjunct, or even replacement, for opioids. However, uptake of NSAIDs has been slow due to concern for side effects, including bleeding. We sought to understand the risk of bleeding caused by NSAIDs in the perioperative period. A physician-librarian team performed a search of electronic databases (MEDLINE, EMBASE), using search terms covering the targeted intervention (use of NSAIDs) and outcomes of interest (surgical complications, bleeding), limited to English language articles of any date. We performed a systematic review and meta-analysis of the data. A total of 2,521 articles were screened, and 229 were selected on the basis of title and abstract for detailed assessment. Including reference searching, 74 manuscripts met inclusion criteria spanning years 1987-2019. These studies included 151,031 patients. Studies included 12 types of NSAIDs, the most common being ketorolac, diclofenac, and ibuprofen, over a wide-range of procedures, from otorhinolaryngology (ENT), breast, abdomen, plastics, and more. More than half were randomized control trials. The meta-analyses for hematoma, return to the operating room for bleeding, and blood transfusions showed no difference in risk in any of 3 categories studied between the NSAID vs non-NSAID groups (p = 0.49, p = 0.79, and p = 0.49, respectively). Quality scoring found a wide range of quality, with scores ranging from lowest quality of 12 to highest quality of 25, out of a total of 27 (average = 16). NSAIDs are unlikely to be the cause of postoperative bleeding complications. This literature covers a large number of patients and remains consistent across types of NSAIDs and operations.


An infant surgical table for laser photocoagulation: ergonomic improvement analysis.
Current methods of treatment for retinopathy of prematurity, using laser photocoagulation, require surgeons to assume awkward standing positions, which can result in occupational injury. A new infant surgical table was designed for improving this surgical procedure. To quantify its benefits, an ergonomic comparison of the standard and modified procedures was carried out, using specialized checklists, Nordic Musculoskeletal Questionnaires, and analysis of videotaped procedures using an Ovako Working Posture Analysing System method. Analysis of the typical laser photocoagulation procedure revealed a high risk for cumulative trauma disorders. The majority of the risk factors were lowered considerably with use of the new table. Improvement was largely due to the new table allowing seated postures during surgery, relieving muscular stress on the back, shoulders and legs. This study demonstrates risk reduction through engineering design of new medical devices, and illustrates how combining different assessment approaches can help evaluate ergonomic impact of medical technologies.


[Seasonal incidence of extrauterine pregnancy].
Ectopic pregnancy (EP) is becoming a more and more frequent problem in many developed countries, but the cause of such increase is not fully understood. Improved diagnosis certainly explains part of it, but also the changing lifestyle and sexual habits might play an important role. During the years 1990-1993, 56 women were admitted into the Divisione di Ostetricia e Ginecologia of the Istituto per l'Infanzia of Trieste because of EP diagnosed on the basis of clinical history and signs, transvaginal echography and serum HCG levels. 33 of them underwent surgical operation for the presence of severe abdominal pain, hemoperitoneum greater than 50 cc, and increases HCG level. The other 23 women were treated conservatively. Taking into account the approximate date of conception both for ectopic and other pregnancies, the monthly incidence of EP was computed and showed a statistically significant peak during the summer period (14.4%) compared to the other periods (6%). The increased frequency of EP among pregnancies started during the warmer months is interesting not only as a descriptive result, but also because it may contribute to a better understanding of the conditions under which the ectopic development of the fertilized ovum may take place.


Whey protein sweetened with Stevia rebaudiana Bertoni (Bert.) increases mitochondrial biogenesis markers in the skeletal muscle of resistance-trained rats.
A combination of resistance training and whey protein supplementation is a common practice among athletes and recreational exercisers to enhance muscle growth and strength. Although their safety as food additives is controversial, artificial sweeteners are present in whey protein supplements. Thus, natural sweeteners extracted from the leaves of Stevia rebaudiana are a potential alternative, due to their safety and health benefits. Here, we investigated the effects of whey protein sweetened with S. rebaudiana on physical performance and mitochondrial biogenesis markers in the skeletal muscle of resistance-trained rats. Forty male Wistar rats were distributed into four groups: sedentary rats, trained rats, trained rats receiving whey protein and trained rats receiving whey protein sweetened with S. rebaudiana leaf extracts. Resistance training was performed by climbing a ladder 5 days per week, during 8-weeks. The training sessions consisted of four climbs carrying a load of 50, 75, 90, and 100% of the maximum load-carrying capacity which we determined before by performing a maximum load-carrying test for each animal. After this period, we collected plasma and tissues samples to evaluate biochemical, histological and molecular (western blot) parameters in these rats. Dietary supplementation with whey protein sweetened with S. rebaudiana significantly enhanced the maximum load-carrying capacity of resistance-trained rats, compared with non-sweetened whey protein supplementation. This enhanced physical performance was accompanied by an increase in the weight of the gastrocnemius and soleus muscle pads. Although the muscle pad of the biceps brachii was not altered, we observed a significant increase in PGC-1α expression, which was followed by a similar pattern in TFAM protein expression, two important mitochondrial biogenesis markers. In addition, a higher level of AMPK phosphorylation was observed in these resistance-trained rats. Finally, supplementation with whey protein sweetened with S. rebaudiana also induced a significant decrease in retroperitoneal adipocyte diameter and an increase in the weight of brown adipose tissue pads in resistance-trained rats. The addition of Stevia rebaudiana leaf extracts to whey protein appears to be a potential strategy for those who want to increase muscular mass and strength and also improve mitochondrial function. This strategy may be useful for both athletes and patients with metabolic disorders, such as obesity and type 2 diabetes.


The Christian Medical Commission and the development of the World Health Organization's primary health care approach.
The primary health care approach was introduced to the World Health Organization (WHO) Executive Board in January 1975. In this article, I describe the changes that occurred within WHO leading up to the executive board meeting that made it possible for such a radical approach to health services to emerge when it did. I also describe the lesser-known developments that were taking place in the Christian Medical Commission at the same time, developments that greatly enhanced the case for primary health care within WHO and its subsequent support by nongovernmental organizations concerned with community health.


The prevalence of depression symptoms after smoking cessation: a systematic review and meta-analysis.
Objectives: Smoking cessation can have positive effects on people's health, especially mental health. This study aimed to address the prevalence of depression in the smoking cessation population. Methods: In the present meta-analysis study, PRISMA protocol was used. Two databases, PubMed and Scopus, were selected. Articles in these two databases in English were targeted and the search was limited to July 2020. First, the results related to the prevalence of depression were calculated. The results were pooled. Results: Forty-nine articles with different designs were eligible for meta-analysis. The prevalence of depression in the smoking cessation population was 18% and the confidence interval was 14-22%. The highest depression prevalence was in Asia and Europe, followed by America. The prevalence of major depression in the smoking cessation population was 15% and the prevalence of depressive symptoms was 17%. The smoking cessation population had a lower odds of depression than current smokers (OR= 0.63 CI = 0.54-0.75; I2 83.9%). There was little evidence for publication bias. Discussion: The status of depression in the smoking cessation population is different from that of nonsmokers and current smokers. Therefore, in terms of health policy and encouraging people to smoking cessation, the issue of its positive effects on mental health should be emphasized.


Non-atopic intrinsic asthma and the 'family tree' of chronic respiratory disease syndromes.
We present a scheme below in which the most common forms of inflammatory diseases of the respiratory tract, notably atopic and non-atopic asthma and COPD, are depicted as separate offshoots from a common 'at-risk' pathway underpinned by genotypes related to aberrations in control of host defence and tissue repair mechanisms. We propose that entrance into this pathway is initially programmed by environmental experience during infancy and early childhood, in particular by severe lower respiratory tract infection, and that further progression towards expression of specific disease phenotype(s) is determined by the nature, timing and frequency of additional environmental insults subsequently encountered. At the one extreme, early sensitization of at-risk subjects to aeroallergens can potentially drive rapid progression towards expression of the atopic asthmatic phenotype under the dual onslaught of inflammatory responses to allergens/pathogens. At the opposite end of the spectrum the drip-feed effects of occasional infections on respiratory function(s) are amplified over a longer time frame by inflammation resulting from exposure to tobacco smoke and/or related chemical pollutants. Non-atopic asthma is envisaged to fit between these two extremes, being driven essentially by the downstream effects of respiratory infections alone in at-risk subjects. An important common factor in all three disease phenotypes is that acute exacerbations are typically driven by infections, the host responses to which display a characteristic T-helper type 2-like footprint, which in our view points to underlying genotype(s) which result in unbalanced host responses to respiratory pathogens.


Qualifying the use of RIS data for patient dose by comparison with DICOM header data.
A system was developed in 2008 to calculate patient doses using Radiology Information System (RIS) data and presents these data as a patient dose audit. One of the issues with this system was the quality of user-entered data. It has been shown that Digital Imaging and Communication in Medicine (DICOM) header data can be used to perform dose audits with a high level of data accuracy. This study aims to show that using RIS data for dose audits is not only a viable alternative to using DICOM header data, but that it has advantages. A new system was developed to pull header data from DICOM images easily and was installed on a workstation within a hospital department. Data were recovered for a common set of examinations using both RIS and DICOM header data. The data were compared on a result-by-result basis to check for consistency of common fields between RIS and DICOM, as well as assessing the value of data fields uncommon to both systems. The study shows that whilst RIS is not as accurate as DICOM, it does provide enough accurate data and that it has other advantages over using a DICOM approach. These results suggest that a 'best of both worlds' may be achievable using Modality Performed Procedure Step (MPPS).


A new desensitizing dentifrice--an 8-week clinical investigation.
An 8-week, double-blind, three-way clinical trial compared the dentinal hypersensitivity-reducing effectiveness of a new dentifrice containing 5.0% potassium nitrate and 0.454% stannous fluoride in a silica base (Colgate Sensitive Maximum Strength Toothpaste, Colgate-Palmolive Co.) with a commercially available desensitizing dentifrice containing 5.0% potassium nitrate and 0.243% sodium fluoride in a silica base (Sensodyne Fresh Mint Toothpaste, Block Drug Company, Inc.) and a nondesensitizing dentifrice containing 0.243% sodium fluoride in a silica base (Colgate Winterfresh Gel, Colgate-Palmolive Co.). One hundred nine subjects were stratified into three balanced groups according to gender, age, mean baseline tactile (Yeaple Probe), and thermal (air blast) scores. The test products were randomly assigned to each group with instructions to brush twice daily. Oral examinations with tactile and thermal assessments were repeated after 4 and 8 weeks. The new dentifrice group demonstrated statistically significant improvements in tactile and thermal sensitivity over the two control groups.


Gaussian beams from variable groove depth grating couplers in planar waveguides.
Analysis, fabrication, and characterization of variable groove depth planar waveguide grating couplers are presented. A formula is derived to describe the grating groove depth variation necessary to produce an outcoupled beam of arbitrary profile. A variable depth grating for producing a Gaussian beam profile is fabricated on a waveguide by ion-beam etching through a scanning slit apparatus. A photoresist grating placed on the waveguide provides a mask to define the grating etched onto the waveguide. The near-field irradiance of the outcoupled beam is measured and shown to approach a Gaussian profile.


The prevalence of inflammatory sacroiliitis assessed on magnetic resonance imaging of inflammatory bowel disease: a retrospective study performed on 186 patients.
Magnetic resonance imaging (MRI) colonography or enterography is increasingly used to assess disease activity and complications in inflammatory bowel disease (IBD). However, no study has evaluated the role of this imaging technique to assess sacroiliitis. The primary objective was to assess the prevalence of inflammatory sacroiliitis on MRI performed for IBD investigation. The secondary aim was to elucidate clinico-biological factors associated with the presence of sacroiliitis. This study was performed on 186 patients suffering from IBD followed in a gastroenterology department between 2004 and 2011: 131 with Crohn's disease and 55 with ulcerative colitis. Clinico-biological and endoscopic data were collected and MR enterography or colonography was performed to assess IBD activity on axial and coronal fat suppressed injected T1-weighted sequences. On MRI, sacroiliitis was scored blindly by two independent readers according to ASAS (Assessment of SpondyloArthritis international Society) criteria. The prevalence of inflammatory sacroiliitis was 16.7% (n = 31). Sacroiliitis was bilateral in 14 cases and unilateral in 17 cases. The sacroiliac joints were considered normal in 144 cases and doubtful in 11 cases. Older age and female gender were significantly associated with the presence of sacroiliitis. Other factors such as type of IBD, disease duration and localisation of IBD, surgery history, biological inflammation, bowel disease activity and treatment were not associated with sacroiliitis. Inflammatory sacroiliitis was evidenced by MRI in 16.7% in patients suffering from IBD. Added to clinico-biological data, MRI analysis should contribute to an earlier diagnosis of axial spondylarthritis in patients with IBD.


Measurement delay associated with the Guardian RT continuous glucose monitoring system.
Using compartment modelling, we assessed the time delay between blood glucose and sensor glucose measured by the Guardian RT continuous glucose monitoring system in young subjects with Type 1 diabetes (T1D). Twelve children and adolescents with T1D treated by continuous subcutaneous insulin infusion (male/female 7/5; age 13.1 +/- 4.2 years; body mass index 21.9 +/- 4.3 kg/m(2); mean +/- sd) were studied over 19 h in a Clinical Research Facility. Guardian RT was calibrated every 6 h and sensor glucose measured every 5 min. Reference blood glucose was measured every 15 min using a YSI 2300 STAT Plus Analyser. A population compartment model of sensor glucose-blood glucose kinetics was adopted to estimate the time delay, the calibration scale and the calibration shift. The population median of the time delay was 15.8 (interquartile range 15.2, 16.5) min, which was corroborated by correlation analysis between blood glucose and 15-min delayed sensor glucose. The delay has a relatively low intersubject variability, with 95% of individuals predicted to have delays between 10.4 and 24.3 min. Population medians (interquartile range) for the scale and shift are 0.800 (0.777, 0.823) (unitless) and 1.66 (1.47, 1.84) mmol/l, respectively. In young subjects with T1D, the total time delay associated with the Guardian RT system was approximately 15 min. This is twice that expected on physiological grounds, suggesting a 5- to 10-min delay because of data processing. Delays above 25 min are rarely to be observed.


Processing of kinetically defined boundaries in the cortical motion area MT of the macaque monkey.
1. Electrophysiological recordings of 68 cells in the middle temporal area MT were made in paralyzed and anesthetized macaque monkeys. 2. Testing with our kinetic boundary stimuli always occurred under optimized conditions. To this end, the preferred direction, speed, stimulus position, and stimulus size of each cell were determined by quantitative tests. 3. The orientation selectivity to stationary luminance contrast edges served as a reference by which a response to kinetic boundaries could be compared. We found cells in area MT to be less selective to the orientation of luminance contrast stimuli than to the direction of motion. We confirmed the presence of neurons with preferred orientation aligned with their preferred direction. 4. The responses to kinetic edges defined by motion vectors moving in opposite directions, kinetic gratings with motion vectors in opposite directions, kinetic edges containing coherent motion and a stationary complementary field or coherent motion and a complementary field containing visual dynamic noise were compared. Kinetic boundaries were generated so that the motion vectors moved either parallel or orthogonal to the orientation of the discontinuity. For a cell to be considered as responding to the orientation of a kinetic boundary, it had to exhibit the same preferred orientation when the local motion vectors changed from parallel to orthogonal to the orientation of the kinetic boundary. 5. All cells in area MT changed their preferred orientation by 90 degrees when the coherent motion vectors changed from moving parallel to moving orthogonal to the boundary. This was the case independent of the types of kinetic boundary tested. We concluded that cells in area MT appear to respond to the motion vector over their classical receptive field (CRF) only and were unable to code the orientation of the kinetic boundary. 6. In those cells exhibiting an antagonistic surround, we examined the ability of the cell to code the position of a kinetic boundary. None of the cells tested signaled the position of a kinetic boundary. The side preference of the stimulus of the cells changed from left to right as the motion vectors in the stimulus reversed. This indicates that the cells were only selective for the motion vectors present over their CRF. 7. We found that the directional sensitivity of cells in area MT remained unaltered by the presence of additional motion vectors within the CRF. This suggests that cells in area MT extract a specific motion vector from a spatial configuration of vectors.(ABSTRACT TRUNCATED AT 400 WORDS)


Analytical model for plasmon modes in graphene-coated nanowire.
An analytical model for plasmon modes in graphene-coated dielectric nanowire is presented. Plasmon modes could be classified by the azimuthal field distribution characterized by a phase factor exp(imφ) in the electromagnetic field expression and eigen equation of dispersion relation for plasmon modes is derived. The characteristic of plasmon modes could be tuned by changing nanowire radius, dielectric permittivity of nanowire and chemical potential of graphene. The proposed model provides a fast insight into the mode behavior of graphene-coated nanowire, which would be useful for applications based on graphene plasmonics in cylindrical waveguide.


[Progress of study on brain protective effect and mechanism of Polygonum multiflorum].
This paper reviews the brain protective effect and mechanism of Polygonum multiflorum (PM), its extracts and active component, tetrahydroxystilbene-glucoside (2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside) published in recent decade. They have major effects as calcium channel antagonists, antioxidant, cholinomimetic drugs and cholinesterase inhibitors, as well as actions in regulating cell apoptosis and prolonging the ageing. The brain protective mechanism of PM is multi-target, multi-link and multi-way. Therefore, PM has great applicative value in prevention and treatment of senile neuropathies, such as Alzheimer's disease, Parkinson's disease and vascular dementia, etc.


A primer on physical therapy.
Physical therapy is an important component of today's health care system. Although physical therapists can be found in many inpatient and outpatient settings, they frequently treat patients with common musculoskeletal problems referred by primary care providers. The physical therapy consultation involves an examination by the physical therapist, diagnosis, prognosis, and initiation of a plan of care that includes specific interventions. Specific interventions include therapeutic exercise, functional training, manual techniques, fitting for assistive devices, application of physical agents such as heat or cold, and electotherapeutic modalities. This article will focus on the use of the physical therapy process for common musculoskeletal problems seen in primary care.


CaO-P2O5 glass hydroxyapatite double-layer plasma-sprayed coating: in vitro bioactivity evaluation.
Double-layer composite coatings composed of a P2O5-based glass/Ca10(PO4)6(OH)2 (HA) mixture top layer and a simple HA underlayer, on Ti-6Al-4V substrates, were prepared using a plasma-spraying technique. The in vitro bioactivity of these coatings was assessed by immersion testing in simulated body fluid. Both scanning electron microscopy (SEM) analysis and the ionic solution changes followed by atomic absorption spectroscopy and the molybdenum blue method demonstrated that these composite coatings induce a faster surface Ca-P layer formation than the simple HA coatings used as a control. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Ca-P layer formed was apatite. The combination of SEM and XPS analyses showed that the apatite layer was a calcium-deficient hydroxyapatite with a Ca/P ranging from 1.3 to 1.4 with CO3(2-) groups contained in the structure.


Imaging properties of polydioxanone and titanium ligating clips following para-aortic lymphadenectomy for testicular cancer.
Stainless steel ligating clips produce serious artefacts on CT scans. Polydioxanone (PDS) and titanium ligating clips were used in 15 patients during para-aortic lymphadenectomy for residual metastatic testicular teratoma following chemotherapy, and follow-up CT images were compared with those following the use of stainless steel ligating clips. PDS ligating clips did not produce any artefacts and were progressively absorbed. Titanium ligating clips produced artefacts similar to but less intense than those of stainless steel. PDS ligating clips should be used when follow-up CT scanning is important.


Outbreak of bovine brucellosis in County Clare, Ireland, in 2005.
This paper describes an investigation of an outbreak of bovine brucellosis in County Clare, Ireland, during 2005. It is likely that infection on the index farm was linked to a previous outbreak of brucellosis in County Clare. During March to May 2005, transmission of brucellosis within the herd was rapid; this was facilitated by a range of factors, including close contact between cattle kept in winter housing, and the mixing of animals, both during grazing and at housing, throughout the year. Containment of the disease, including only limited spread to one contiguous herd, was facilitated by the recent construction of a shed for winter housing.