[Food additives and genetically modified food--a risk for allergic patients?].
Adverse reactions to food and food additives must be classified according to pathogenic criteria. It is necessary to strictly differentiate between an allergy, triggered by a substance-specific immunological mechanism, and an intolerance, in which no specific immune reaction can be established. In contrast to views expressed in the media, by laymen and patients, adverse reactions to additives are less frequent than is believed. Due to frequently "alternative" methods of examination, an allergy to food additives is often wrongly blamed as the cause of a wide variety of symptoms and illness. Diagnosing an allergy or intolerance to additives normally involves carrying out double-blind, placebo-controlled oral provocation tests with food additives. Allergic reactions to food additives occur particularly against additives which are organic in origin. In principle, it is possible that during the manufacture of genetically modified plants and food, proteins are transferred which potentially create allergies. However, legislation exists both in the USA (Federal Drug Administration, FDA) and in Switzerland (Ordinance on the approval process for GM food, GM food additives and GM accessory agents for processing) which require a careful analysis before a genetically modified product is launched, particularly where foreign genes are introduced. Products containing genetically modified organisms (GMO) as additives must be declared. In addition, the source of the foreign protein must be identified. The "Round-up ready" (RR) soya flour introduced in Switzerland is no different from natural soya flour in terms of its allergenic potential. Genetically modified food can be a blessing for allergic individuals if gene technology were to succeed in removing the allergen (e.g. such possibilities exist for rice). The same caution shown towards genetically modified food might also be advisable for foreign food in our diet. Luckily, the immune system of the digestive tract in healthy people tolerates foreign antigens. Food allergies in adults occur mainly among those allergic to pollen.


[Experimental study for the anti-fatigue effect of ginseng general ginsenosides P.E. in vivo].
To investigate the effective dose and the possible mechanism of the anti-fatigue effect of ginsenosides and then deduce to the recommended intake of human body. Healthy adult male SD rats intook ginsenosides solution every day through stomach at doses of 50, 150, 250 mg/kg per day, other rats were divided into untreated group and model control group, given equal amount of distilled water for 30 days. Conducted exhaustive swimming experiment for rats except untreated group on the last day of gavage after 30 minutes. Determine the time of the exhaustive swimming, organ coefficient, liver glycogen, muscle glycogen, malonaldehyde (MDA) of liver cells, superoxide dismutase (SOD) of liver cells, Na+ -K+ adenosine triphosphatase (Na+ -K+-ATPase) and Ca2+ -Mg2+ adenosine triphosphatase (Ca2+ -Mg2+-ATPase) of erythrocyte, blood lactate, fumaric reductase. The body weight of rats middle, high dose group were higher than that in model control group after exhaustive swimming (P < 0.05). The time of the exhaustive swimming of high dose group were longer than model control group (P < 0.05). Some organ coefficient of model control group have statistically significant compared with other groups (such as brain, kidney, adrenal, thymus coefficient) (P < 0.05). The activity of SOD of liver cells of untreated group, middle dose group and high dose group were higher than model control group and low dose group (P < 0.05), but the level of MDA of liver cells of them were lower than model control group and low dose group (P < 0.01), the activity of Na+ -K+-ATPase of erythrocyte of untreated group and high dose group were higher than model control group (P < 0.05), but the activity of Ca2+ -Mg2+-ATPase of erythrocyte of each group had no statistical significance (P > 0.05). The level of liver glycogen, muscle glycogen and fumaric reductase of model control group were lower than untreated group and high dose group (P < 0.05). At the experimental conditions, ginsenosides solution of 250 mg/kg can improve the tolerance of rats in fatigue campaign and have the function of anti-fatigue.


[The impact of size and diversity on group process and outputs].
The impact of size and diversity on group process and outputs. This study has analyzed the impact of size and diversity on group processes (group cohesion, effective management of conflicts, and group norms) and outputs (work satisfaction and group effectiveness) in a sample (N= 407) of Mexican workers. It considered two types of diversity: functional and social diversity. Hierarchical regression analysis revealed main effects for size and functional diversity. Size is negatively associated with group norms, effective management of conflicts and job satisfaction. Functional diversity is positively related to group norms and cohesion. However, social diversity was not related to group process and outcomes. The analysis revealed a moderating effect of functional diversity on the relationship between size and job satisfaction. The practical implications of these results are analyzed.


Structural and Environmental Influences Increase the Risk of Sexually Transmitted Infection in a Sample of Female Sex Workers.
Female sex workers (FSWs) have elevated rates of sexually transmitted infections (STIs) including HIV, yet few studies in the United States have characterized the STI burden in this population. Data were derived from the EMERALD study, a structural community-based intervention with FSWs in Baltimore, MD. Participants (n = 385) were recruited through targeted sampling on a mobile van. Prevalent positive chlamydia or gonorrhea infections were determined by biological samples. Multivariable logistic regressions modeled correlates of confirmed positive STI (gonorrhea or chlamydia). Confirmed STI positive prevalence was 28%, 15% chlamydia and 18% gonorrhea. Approximately two-thirds of the sample (64%) was younger than 40 years, one-third (36%) were Black, and 10% entered sex work in the past year. The sample was characterized by high levels of structural vulnerabilities (e.g., housing instability and food insecurity) and illicit substance use. Female sex workers were more likely to have a positive STI if they had financial dependent(s) (P = 0.04), experienced food insecurity at least weekly (P = 0.01), entered sex work in the past year (P = 0.002), and had 6 or more clients in the past week (P = 0.01). Female sex workers were less likely to have a positive STI test result if they were 40 years or older compared with FSW 18 to 29 years old (P = 0.02), and marginally (P = 0.08) less likely with high (vs. low) social cohesion. More than a quarter of FSWs had confirmed chlamydia or gonorrhea. In addition to STI risks at the individual level, STIs are driven by structural vulnerabilities. Results point to a number of salient factors to be targeted in STI prevention among FSWs.


A microfluidic impedance flow cytometer for identification of differentiation state of stem cells.
This paper presents a microfluidic electrical impedance flow cytometer (FC) for identifying the differentiation state of single stem cells. This device is comprised of a novel dual micropore design, which not only enhances the processing throughput, but also allows the associated electrodes to be used as a reference for one another. A signal processing algorithm, based on the support vector machine (SVM) theory, and a data classification method were developed to automate the identification of sample types and cell differentiation state based on measured impedance values. The device itself was fabricated using a combination of standard and soft lithography techniques to generate a PDMS-gold electrode construct. Experimental testing with non-biological particles and mouse embryonic carcinoma cells (P19, undifferentiated and differentiated) was carried out using a range of excitation frequencies. The effects of the frequency and the interrogation parameters on sample identification performance were investigated. It was found that the real and imaginary part of the detected impedance signal were adequate for distinguishing the undifferentiated P19 cells from non-biological polystyrene beads at all tested frequencies. A higher frequency and an opacity index were required to resolve the undifferentiated and differentiated P19 cells by capturing capacitive changes in electrophysiological properties arising from differentiation. The experimental results demonstrated salient accuracy of the device and algorithm, and established its feasibility for non-invasive, label-free identification of the differentiation state of the stem cells.


Complement-fixing islet cell antibodies in type-1 diabetes can trigger the assembly of the terminal complement complex on human islet cells and are potentially cytotoxic.
Forty-one sera of patients with IDDM (insulin-dependent diabetes mellitus) containing complement-fixing islet cell antibodies were analyzed for their ability to activate TCC (terminal complement complex). Eighteen sera were found to promote deposition of TCC on human islets of pancreatic cryostat sections with a nonhomogeneous pattern of distribution corresponding to that of insulin. Activation of TCC by IDDM serum and binding of this complex to islet cells was confirmed using purified islets. Flow cytometric analysis of islet cell treated with a TCC+ IDDM serum showed IgG binding to the cell surface. The same serum had a cytotoxic effect on islet cells in the presence of human C. These results obtained with a homologous system of C activation by IDDM sera suggest that TCC may contribute, at least in part, to the pancreatic beta cell damage.


Antibody and T cell responses of patients with adenocarcinoma immunized with mannan-MUC1 fusion protein.
Mucin 1 (MUC1) is a large complex glycoprotein that is highly expressed in breast cancer, and as such could be a target for immunotherapy. In mice, human MUC1 is highly immunogenic, particularly when conjugated to mannan, where a high frequency of CD8(+) MHC-restricted cytotoxic T lymphocytes is induced, accompanied by tumor protection. On this basis, a clinical trial was performed in which 25 patients with advanced metastatic carcinoma of breast, colon, stomach, or rectum received mannan-MUC1 in increasing doses. After 4 to 8 injections, large amounts of IgG1 anti-MUC1 antibodies were produced in 13 out of 25 patients (with antibody titers by ELISA of 1/320-1/20,480). Most of the antibodies reacted to the epitopes STAPPAHG and PAPGSTAP. In addition, T cell proliferation was found in 4 out of 15 patients, and CTL responses were seen in 2 out of 10 patients. Mannan-MUC1 can immunize patients, particularly for antibody formation, and to a lesser extent, cellular responses. It remains to be seen whether such responses have antitumor activity.


The Relationship Between Business Environment and Single Champion Enterprise Entrepreneurship.
What kind of business environment can produce high single champion enterprise entrepreneurship is a new issue for discussion in research on entrepreneurship. Based on institutional configuration theory and the fcQCA method, the present paper analyses the relationship between the business environment and single champion enterprise entrepreneurship from the perspective of configuration. This paper studies the role of the business environment in 80 case cities all over the country in promoting high single champion enterprise entrepreneurship and discusses three business environment configurations concerning high single champion enterprise entrepreneurship and two configurations concerning non-high single champion enterprise entrepreneurship. Three typical business environment element configurations can promote high single champion enterprise entrepreneurship, namely, the market innovation type dominated by multiple resources, the financial service-driven type assisted by resources, and the market-driven type led by financial services, which reflects the significance of financial services and the market environment.


Osteoblastoma response to chemotherapy.
An 8-year-old boy had an osteoblastoma of the body of C2. After attempted excision, the tumor recurred rapidly and massively. The tumor shrank progressively after treatment with high-dose methotrexate, doxorubicin, and cisplatin. The patient has stopped treatment and been stable for 33 months. Surgical excision remains the treatment of choice for osteoblastoma. Chemotherapy may be useful in selected patients with a recurrent, aggressive tumor or in patients with surgically inaccessible disease.


Disease progression in cardiac morphology and function in heart failure: ATTR cardiac amyloidosis versus hypertensive left ventricular hypertrophy.
Transthyretin cardiac amyloidosis (ATTR-CA) is today more frequently recognized but the rate of progression of cardiac dysfunction is not well established. The aim of this study is to investigate the nature of cardiac structure and function changes, over time, in a retrospective cohort of ATTR-CA patients. Fifty-one patients with ATTR-CA (mean age 78 ± 7 years, 30 females) were compared with 20 patients with heart failure but no amyloidosis (HFnCA) (mean age 76 ± 7 years, 5 females), all with septal thickness > = 14 mm. All patients underwent DPD scintigraphy and an echocardiogram (Echo 2) which was compared with a previous echocardiographic examination (Echo 1), performed at least 3 years before. Over the follow-up period, the interventricular septal thickness (IVST) and relative wall thickness (RWT) in ATTR-CA increased from 16 (4) to 18 (5) mm and from 0.51 (0.17) to 0.62 (0.21) respectively, p < 0.001 for both, by a mean increase of 0.4 mm/year and 0.03 mm/year, (p = 0.009 and p = 0.002 compared with HFnCA), respectively. RWT > 0.45 (AUC = 0.77) and RELAPS > 2.0 (AUC 0.86) both predicted positive DPD diagnosis for ATTR-CA. In ATTR-CA patients, the overtime-increase in RWT and IVST was worse than that seen in patients with heart failure but no cardiac amyloidosis. Also, RWT and relative apical sparing predicted diagnosis of ATTR-CA, thus could strengthen the use of follow-up echocardiographic findings as red flag for the diagnosis of ATTR-CA.


Phenotypic and genetic spectrum of Danish patients with ABCA4-related retinopathy.
Pathogenic variations in the ABCA4 gene were originally recognized as genetic background for the autosomal recessive disorders Stargardt disease and fundus flavimaculatus, but have expanded to embrace a diversity of retinal diseases, giving rise to the new diagnostic term, ABCA4-related retinopathy. Diagnostic genotyping of ABCA4 is complicated by the large size of the gene and the existence of approximately 600 known pathogenic variations, along with numerous rare polymorphisms. A commercial diagnostic array-based assay has been developed targeting known mutations, however a conclusive genetic diagnosis must rely on a comprehensive genetic screening as the mutation spectrum of ABCA4-related retinopathies continues to expand. Among 161 patients with a Stargardt-related phenotype previously assessed with the commercial ABCA4 mutation microarray, we analyzed the ABCA4 gene with High-resolution melting (HRM) in patients in whom the array analysis identified either a heterozygous mutation (n = 50) or no mutation (n = 30). The HRM method detected each of the already known mutations and polymorphisms. We identified the second ABCA4 mutation in 31 of 50 heterozygous patients (62%). Several novel mutations were identified of which four were identified multiple times. The recurrent novel mutations were subsequently assessed among the 30 patients with possible ABCA4-related diseases, previously found to be negative for known ABCA4 mutations by array analysis. In total, 30 different mutations were identified of which 21 have not been described before. Scandinavian patients with ABCA4-related retinopathy appear to have a distinct mutation spectrum, which can be identified in patients of diverse clinical phenotypes.


An epidemiologic study of sleep problems among adolescents in North Taiwan.
To investigate the prevalence of sleep problems and their association with daytime sleepiness among Taiwanese adolescents by use of a validated questionnaire. This is a cross-sectional, community based study with self-reported sleep questionnaires. Completed questionnaires from 1939 adolescent subjects from schools in Lin-Kou district (Taipei, Taiwan) (96.7% responded); 1906 valid questionnaires (62.3% girls) were analyzed. The randomly selected classes included elementary grade 6 (age range: 12-13 years), junior high school (age range: 14-16 years) and senior high school students (age range: 17-18 years). The mean sleep duration on weekdays was 7.35±1.23 h and on weekends 9.38±1.62 h. Weeknight sleep decreased significantly with increasing school grade (6.87±1.14 h for high school seniors). There was a trend towards increased daytime sleepiness for students in higher school grade levels. Daytime sleepiness directly correlated with shorter total sleep time (TST) on weekdays, longer TST on weekends, snoring, insomnia and nightmares. Coffee intake, smoking, periodic leg movement/restless legs syndrome, body mass index (BMI), mouth breathing and breathing problems were indirect factors that induced daytime sleepiness. Pearson correlation showed no significant correlation between the TST during the weekday and BMI (-0.047, p=0.079) or body weight (BW) (-0.048, p=0.072). But it showed significant negative correlation (-0.103, p=0.0001) for increasing total sleep time on the weekend and decreasing BMI. Daytime sleepiness correlated with the shorter TST on weekdays, longer TST on weekends, snoring, insomnia and nightmares. There is no significant correlation between the weekday TST and BMI or BW. Meals and food intake of children are still traditional and have not changed as much in Taiwan as in some other western countries, and compared to a similar survey performed 12 years ago in Taiwan among junior high school students, sleep duration was not significantly different but reduced due to school demands.


Higher circulating irisin levels in patients with polycystic ovary syndrome:a meta-analysis.
Insulin resistance (IR) has been reported to be highly associated with the pathogenesis of polycystic ovary syndrome (PCOS). Although irisin, a newly identified myokine that may be closely associated with IR, has been implicated in the development of PCOS, the results are still ambiguous. We performed this meta-analysis to compare the circulating irisin levels between PCOS and healthy women and assess the association of irisin with IR. Published works were retrieved from PubMed and Embase databases using combinations of 'irisin' and ('polycystic ovary syndrome' or 'PCOS'). Eight studies involving 1918 PCOS patients and 528 controls were included in the meta-analysis. Publication bias was observed using a funnel plot and Egger's regression asymmetry test. The pooled data indicated that the levels of irisin were at least 45.78 ng/ml [95% confidence interval (CI)] (12.45, 79.12, p = .007) higher in patients with PCOS than that in the healthy controls. Additionally, we did not observe a significant correlation between circulating irisin levels and IR in study populations, although the results may not be reliable for small sample sizes. The current meta-analysis suggested that irisin might contribute to the development of PCOS independent of IR.


Acculturation and perinatal outcomes in Mexican immigrant childbearing women: an integrative review.
Despite several sociocultural factors associated with increased risk for low birth weight and infant mortality, the rate for infants born to first-generation and less acculturated Mexican immigrant women is the same as that of non-Hispanic whites, and half that of African Americans with similar risks. It appears that sociocultural rather than genetic variables are the primary factors associated with this phenomenon. Higher levels of acculturation to North American values and lifestyle in Mexican American childbearing women have been correlated with poor perinatal outcomes, including low birth weight. Acculturation is emerging as an important variable that should be considered when providing health care to Mexican immigrant childbearing women and their families.


Partner violence and HIV testing uptake among Ethiopian women.
Guided by the Anderson Behavioral Model, the researchers in this study examined the association between emotional violence and HIV testing uptake among women, using the 2016 Ethiopian Demographic and Health Survey. Only 3,637 women between the ages of 15 and 49 years were included. In the bivariate analysis, emotional violence, reported by 42.6% of respondents, was associated with prior HIV testing. In the multiple logistic regression model, emotional violence was not associated with HIV testing uptake among women (p > 0.05). Our findings suggest that efforts to improve women's socio-economic status and decision-making autonomy may be more relevant to increasing HIV testing uptake.


Changes in Lumbosacral Anatomy and Vertebral Numbering in Patients with Thoracolumbar and/or Lumbosacral Transitional Vertebrae.
The presence of a thoracolumbar transitional vertebra (TLTV) and/or lumbosacral transitional vertebra (LSTV) may cause wrong-site surgery and problems while measuring spinopelvic parameters, including pelvic incidence and lumbar lordosis. The Castellvi classification of LSTV addresses coronal images but not sagittal or axial images. Therefore, it is unclear how LSTV differs from the normal lumbosacral anatomy. We aimed to investigate the lumbosacral anatomy and vertebral numbering in patients with TLTV and/or LSTV. We performed computed tomography (CT) to identify TLTV, to number presacral vertebrae accurately, and to analyze morphological differences in each LSTV type. The medical records of 880 patients who underwent spinopelvic fixation between July 2014 and March 2020 were evaluated for TLTV and LSTV. Castellvi LSTVs (above the promontory on the arcuate line of the ilium) and our newly proposed LSTV ("S6 LSTV," with 6 sacral vertebrae and 5 foramina below the promontory) were analyzed. The anatomical location of the lowest thoracic vertebra was defined, and TLTV with dysplastic ribs was identified. Each LSTV type was examined for its morphological features on sagittal and axial CT images. LSTV was observed in 111 (12.6%) of 880 patients. Castellvi type-III LSTV was the most common (42 [37.8%] of 111), followed by S6 LSTV (37 [33.3%] of 111). TLTV was associated with LSTV (87 [78.4%] of 111) and was commonly identified at T13 (59 [67.8%] of 87). On sagittal CT images, the lumbosacral transitional anatomy of Castellvi LSTVs resembled that of normal L5-S1, and the lumbosacral transitional anatomy of S6 LSTV resembled that of normal S1-S2. When comparing the S1 upper segments on axial CT images, most Castellvi LSTVs exhibited S2-like appearances and most S6 LSTVs exhibited L5-like appearances. Although LSTV possesses L5 and S1 features, Castellvi LSTVs have more L5 elements than S1 elements. The converse is true for S6 LSTV. At least for the Castellvi type-IIIb LSTV, the vertebra below the Castellvi type-IIIb LSTV should be recognized as S1, but clinically it is better to recognize it as S2. Overlooking TLTV may cause problems in vertebral numbering due to coexisting LSTV. Three-dimensional CT images are suitable for detecting transitional vertebrae. This study reveals their morphological features on axial CT images and their lumbosacral anatomy on sagittal CT images.


Protecting a quantum memory for a photonic polarization qubit in a cold atomic ensemble by dynamical decoupling.
We report an experimental demonstration of storage of photonic polarization qubit (PPQ) protected by dynamical decoupling (DD). PPQ's states are stored as a superposition of two spin waves by electromagnetically-induced-transparency (EIT). Carr-Purcell-Meiboom-Gill (CPMG) DD sequences are applied to the spin-wave superposition to suppress its decoherence. Thus, the quantum process fidelity remains better than 0.8 for up to 800 μs storage time, which is 3.4-times longer than the corresponding storage time of ~180 μs without the CPMG sequences. This work is a key step towards the storage of single-photon polarization qubit protected by the CPMG sequences.


Target identification of a novel unsymmetrical 1,3,4-oxadiazole derivative with antiproliferative properties.
1,3,4-Oxadiazole derivatives are widely used in research on antineoplastic drugs. Recently, we discovered a novel unsymmetrical 1,3,4-oxadiazole compound with antiproliferative properties called 2j. To further investigate its possible targets and molecular mechanisms, RNA-seq was performed and the differentially expressed genes (DEGs) were obtained after treatment. Data were analyzed using functional (Gene Ontology term) and pathway (Kyoto Encyclopedia of Genes and Genomes) enrichment of the DEGs. The hub genes were determined by the analysis of protein-protein interaction networks. The connectivity map (CMap) information provided insight into the model action of antitumor small molecule drugs. Hub genes have been identified through function gene networks using STRING analysis. The small molecular targets obtained by CMap comparison showed that 2j is a tubulin inhibitor and it acts mainly affecting tumor cells through the cell cycle, FoxO signaling pathway, apoptotic, and p53 signaling pathways. The possible targets of 2j could be TUBA1A and TUBA4A. Molecular docking results indicated that 2j interacts at the colchicine-binding site on tubulin.


Efficient algorithms for covariate analysis with dynamic data using nonlinear mixed-effects model.
Nonlinear mixed-effects modeling is one of the most popular tools for analyzing repeated measurement data, particularly for applications in the biomedical fields. Multiple integration and nonlinear optimization are the two major challenges for likelihood-based methods in nonlinear mixed-effects modeling. To solve these problems, approaches based on empirical Bayesian estimates have been proposed by breaking the problem into a nonlinear mixed-effects model with no covariates and a linear regression model without random effect. This approach is time-efficient as it involves no covariates in the nonlinear optimization. However, covariate effects based on empirical Bayesian estimates are underestimated and the bias depends on the extent of shrinkage. Marginal correction method has been proposed to correct the bias caused by shrinkage to some extent. However, the marginal approach appears to be suboptimal when testing covariate effects on multiple model parameters, a situation that is often encountered in real-world data analysis. In addition, the marginal approach cannot correct the inaccuracy in the associated p-values. In this paper, we proposed a simultaneous correction method (nSCEBE), which can handle the situation where covariate analysis is performed on multiple model parameters. Simulation studies and real data analysis showed that nSCEBE is accurate and efficient for both effect-size estimation and p-value calculation compared with the existing methods. Importantly, nSCEBE can be >2000 times faster than the standard mixed-effects models, potentially allowing utilization for high-dimension covariate analysis for longitudinal or repeated measured outcomes.


Neurocognitive correlates of response to treatment in formal thought disorder in patients with first-episode schizophrenia.
To examine the independent contribution of executive versus semantic function to improvement in formal thought disorder after initial stabilization in a first-episode sample. Neurocognitive deficits have been suggested to predict treatment response in patients with first-episode schizophrenia. However, studies targeting putative neurocognitive mechanisms to explore improvement in positive psychotic symptoms and especially formal thought disorder are lacking. Formal thought disorder symptoms in 81 first-episode patients with schizophrenia or schizoaffective disorder either showed significant improvement (responders > 60% change) or not (nonresponders < 60%) 6 months after initial stabilization of symptoms. These two groups were compared on neuropsychologic (n = 16), clinical (n = 15), and volumetric measures of the frontal and temporal lobes (n = 5) in univariate analyses. The variables that significantly differed between these two groups were used in a forward binary logistic regression analysis. As compared with nonresponders, responders were younger at time of testing, had higher verbal intelligence and reading achievement scores, higher scores on the arithmetic subtest of the Wechsler Adult Intelligence Scale-Revised, and lower number of perseverative responses on the Wisconsin Card Sort Test. Responders also had larger frontal lobe volumes than nonresponders. Only two measures (perseverative responses on the Wisconsin Card Sort Test and age at testing) entered the regression equation. Measures of semantic competency and volumetric measures of the temporal lobes were not associated with formal thought disorder improvement. Neurocognitive deficits are associated with treatment response in formal thought disorder in first-episode patients with schizophrenia. The improvement in formal thought disorder is more strongly linked to executive than semantic function in this sample, pointing to the salience of frontal systems in treatment response in positive psychotic symptoms.


Preschool teachers as agents of oral health promotion: an intervention study in Sri Lanka.
According to National Oral Health Survey reports and research, Early Childhood Caries has been identified as a serious public health problem in Sri Lanka. More than 65% of preschool-aged children have dental decay and only 2% of them have had treatment. With proper interventions and commitment from public health personnel and responsible community leaders this should be a largely preventable disease. An intervention study was conducted among preschool teachers in the District of Colombo, Sri Lanka, to assess their influence on oral health promotion in the school environment. All the available 52 preschools and all 72 teachers registered under a local government authority were involved in the study. Preschools were divided into intervention group and control group based on geographically defined areas. The intervention included training preschool teachers using a manual covering health education, health promotion, incorporation of oral-health-friendly activities into the preschool curriculum, and hands-on experience of oral examination. Pre- and post- assessments were conducted with a 6 month interval. After 6 months, the median oral health knowledge score of the intervention group improved from 55 to 72 (p = 0.005) and the mean score for oral health related practices from 32 to 35 (p = 0.032). The variables: oral-health-friendly preschool environment (p = 0.02), availability of brushing facilities (p = 0.005) and availability of information, education and communication materials related to oral health (p = 0.004) were significantly different between the two groups after 6 months. Oral health promotion activities can be effectively instilled in a pre-school environment by the education of teachers.


Risk factors associated with unplanned caesarean section in women with placenta previa: a cohort study.
Unplanned/emergency caesarean section (CS) can lead to an increased risk of increased risk of adverse maternal and perinatal outcomes. This prospective observational study was conducted in a tertiary centre in South India to determine the factors associated with increased risk of unplanned/emergency CS among women with placenta previa (PP). Primary outcome was the unplanned CS defined as emergency CS performed, prior to the scheduled date of delivery, for profuse vaginal bleeding or onset of labour pains. Obstetric morbidity and maternal-foetal outcomes were also compared between major and minor degree of PP. Major degree PP (OR 3.56; 95% CI: 1.73-7.32), first episode of bleeding at less than 29 weeks of gestation (OR 6.25; 95% CI: 2.14-18.24), and the haemoglobin level at admission (OR: 0.72; 95% CI: 0.57-0.91) were found to be associated with higher odd for undergoing unplanned CS. Identifying these women at high risk of unplanned CS, especially in limited resource setting, helps for a timely and early referral to tertiary centres with expertise to manage complications along with facilities for blood transfusion and interventional radiology can help to optimise maternal and perinatal outcomes.Impact StatementWhat is already known on this subject? With increasing numbers of caesarean sections (CSs) and assisted reproductive techniques, the rate of PP is constantly on the rise. Unplanned CS is associated with increased risk of adverse maternal and perinatal complications.What do the results of this study add? Nearly, 40% among those who underwent CS were unplanned. Major degree placenta previa (PP) (OR 3.56; 95% CI: 1.73-7.32), first episode of bleeding at less than 29 weeks of gestation (OR 6.25; 95% CI: 2.14-18.24), and the haemoglobin level at admission (OR: 0.72; 95% CI: 0.57-0.91) were found to be associated with higher odd for undergoing unplanned CS.What are the implications of these findings for clinical practice and/or further research? Identifying women with PP at high risk of unplanned CS, especially in limited resource setting, helps for a timely and early referral to tertiary centres with expertise to manage complications, facilities for blood transfusion and interventional radiology, which optimise maternal and perinatal outcomes.


A six-month double-blind trial to compare the efficacy and safety of meloxicam 7.5 mg daily and naproxen 750 mg daily in patients with rheumatoid arthritis.
Meloxicam is a new non-steroidal anti-inflammatory drug (NSAID) which preferentially inhibits cyclooxygenase-2 over cyclooxygenase-1. A double-blind parallel-group trial compared meloxicam 7.5 mg once daily (n = 199) with naproxen 750 mg (n = 180) in rheumatoid arthritis. There was no significant difference between the groups regarding the primary efficacy variables (global efficacy assessment by patient and investigator, number of painful/tender and swollen joints) and eight of the ten secondary efficacy endpoints. Only the swollen joint severity index and the number of discontinuations due to lack of efficacy favoured naproxen 750 mg significantly over meloxicam 7.5 mg. Meloxicam was better tolerated in the gastrointestinal (GI) tract, with fewer GI adverse events in the meloxicam-treated group (30.3%) than in the naproxen-treated group (44.7%), where two patients developed ulcers. No ulcers were seen in meloxicam patients. Significantly more patients discontinued due to GI adverse events in the naproxen group. Additionally, there was a significant decrease in haemoglobin and a significant increase in serum creatinine and urea in the naproxen group compared with the meloxicam group. In conclusion, meloxicam 7.5 mg once daily is a promising treatment in rheumatoid arthritis, with efficacy comparable to naproxen 750 mg. Meloxicam has the advantage of a significantly lower incidence of GI and renal side effects.


Total synthesis of pteridic acid A.
A convergent approach to the total synthesis of pteridic acid A, having potent plant growth regulator activity, is described. Key steps include the desymmetrization of bicyclic olefin with Brown's chiral hydroboration, acid-mediated spiroketalization, and zirconium-catalyzed ethylmagnesation protocol to install the ethyl stereocenter at C14.


Angiotensin-converting enzyme gene polymorphism in hypertensive rural population of Haryana, India.
Essential hypertension is a complex genetic disorder influenced by diverse environmental factors. Of the various physiological pathways affecting the homeostasis of blood pressure, the renin-angiotensin system (RAS) is known to play a critical role. Angiotensin-I converting enzyme (ACE) is a significant component of RAS and an insertion/deletion (I/D) polymorphism in its gene has been implicated in predisposition to hypertension. The present study is aimed to determine the association, if any, of ACE I/D polymorphism with essential hypertension in a rural population of Haryana, India. The blood samples were collected from the patients visiting M. M. Institute of Medical Sciences, Mullana, Haryana. DNA from the patients (106) and control (110) specimens were isolated, amplified by PCR and analyzed employing agarose gel electrophoresis. There was no significant difference in the distribution of DD, II and I/D genotypes of ACE polymorphism in essential hypertensive patients (28.8, 25.5, and 46.2%) and their ethnically matched normal control (24.5, 30, and 45.5), respectively. The two groups also presented with very similar allelic frequencies and were also found to be in Hardy-Weinberg equilibrium. The present study demonstrates that ACE I/D polymorphism is not a risk factor for essential hypertension in the hitherto unstudied rural population of Haryana.


A spatial model of the knee for the preoperative planning of knee surgery.
A model on the spatial mechanical behaviour of the passive knee is presented. The femoral articular surfaces were represented by generalized, sagittally elliptical, toroidal surfaces. The medial and lateral tibial articular surfaces were represented by a dished spherical surface and the lower hemihyperbolic region of a torus respectively. Anatomical articular cartilage, knee ligaments and the posterior capsule were represented by spring-like deformable elements with non-linear load versus deflection characteristics. All the forces that act on the femur relative to the tibia were represented by three orthogonal forces and three associated moments. Spatial, articulation-dependent femorotibial kinematic constraint equations of the passive knee were formulated in an analytically explicit manner, based on the natural coordinates of the articular surfaces. The constraint equations were solved algebraically in closed form. Equations were derived that describe spatial femoro-tibial motion, ligament length, ligament strain, ligament-based elastic potential energy and the quasi-static equilibrium of the passive knee. Software was written, simulations on the motion characteristics and load versus deflection characteristics of the knee were carried out and graphical results were presented. The simulation of planar flexion/extension was almost spontaneous. The time taken to simulate spatial six-degree-of-freedom femoro-tibial motion was less than 2.5 min. The models were found to be capable of representing real-life passive knees to a high degree of satisfaction. It has been demonstrated that the models can provide knee surgeons with additional information on major aspects of the preoperative planning of knee surgery. The models can be used to enhance the preoperative planning of ligament reconstruction, articular surfaces related surgery, osteotomy and patellar tendon transfer surgery.


AntagomiR-19a Induced Better Responsiveness to Bortezomib in Myeloma Cell Lines.
Multiple myeloma (MM) is the clonal proliferation of neoplastic plasma cells in the bone marrow. Although bortezomib (BTZ) is a crucial drug for the treatment of MM, drug resistance is a major problem. OncomiR-19a plays an oncogenic role in many cancers, including MM; however, the function of miR-19a in the pathogenesis of MM and drug resistance has not been completely identified. The present research aims to investigate the inhibition of miR-19a by an antagomir to determine BTZ responsiveness, and determine if miR-19a can be a prognostic biomarker for MM. In this experimental study, viability and apoptosis of myeloma cells were analysed by the colorimetric 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide (MTT) and Annexin V/propidium iodide (PI) flow cytometry assays. Quantitative real-time polymerase chain reaction (qRT-PCR) was implemented to evaluate the expression levels of miR-19a, its targets SOCS3, STAT3, B-cell lymphoma 2 (BCL-2), PTEN and CDKN1A (antiapoptotic and cell cycle related genes) at the mRNA level. miR-19a was downregulated and exacerbated in transfected cells treated with BTZ. The rate of apoptosis in the myeloma cells after BTZ treatment considerably increased, which indicated an increase in the mRNA of SOCS3, PTEN, BCL-2, and CDKN1. A decrease in STAT3 was also observed. OncomiR-19a, as a biomarker, may induce better responsiveness to BTZ in myeloma cell lines through its targets SOCS3, STAT3 and PTEN. In the future, this biomarker may provide new therapeutic targets for MM.


Renoprotection From Diabetic Complications in OVE Transgenic Mice by Endothelial Cell Specific Overexpression of Metallothionein: A TEM Stereological Analysis.
We previously demonstrated that OVE transgenic diabetic mice are susceptible to chronic complications of diabetic nephropathy (DN) including substantial oxidative damage to the renal glomerular filtration barrier (GFB). Importantly, the damage was mitigated significantly by overexpression of the powerful antioxidant, metallothionein (MT) in podocytes. To test our hypothesis that GFB damage in OVE mice is the result of endothelial oxidative insult, a new JTMT transgenic mouse was designed in which MT overexpression was targeted specifically to endothelial cells. At 60 days of age, JTMT mice were crossed with age-matched OVE diabetic mice to produce bi-transgenic OVE-JTMT diabetic progeny that carried the endothelial targeted JTMT transgene. Renal tissues from the OVE-JTMT progeny were examined by unbiased TEM stereometry for possible GFB damage and other alterations from chronic complications of DN. In 150 day-old OVE-JTMT mice, blood glucose and HbA1c were indistinguishable from age-matched OVE mice. However, endothelial-specific MT overexpression in OVE-JTMT mice mitigated several DN complications including significantly increased non-fenestrated glomerular endothelial area, and elimination of glomerular basement membrane thickening. Significant renoprotection was also observed outside of endothelial cells, including reduced podocyte effacement, and increased podocyte and total glomerular cell densities. Moreover, when compared to OVE diabetic animals, OVE-JTMT mice showed significant mitigation of nephromegaly, glomerular hypertrophy, increased mesangial cell numbers and increased total glomerular cell numbers. These results confirm the importance of oxidative stress to glomerular damage in DN, and show the central role of endothelial cell injury to the pathogenesis of chronic complications of diabetes. Anat Rec, 2017. © 2017 Wiley Periodicals, Inc. Anat Rec, 300:560-576, 2017. © 2016 Wiley Periodicals, Inc.


Revisiting Biomarkers of Total-Body and Partial-Body Exposure in a Baboon Model of Irradiation.
In case of a mass casualty radiation event, there is a need to distinguish total-body irradiation (TBI) and partial-body irradiation (PBI) to concentrate overwhelmed medical resources to the individuals that would develop an acute radiation syndrome (ARS) and need hematologic support (i.e., mostly TBI victims). To improve the identification and medical care of TBI versus PBI individuals, reliable biomarkers of exposure could be very useful. To investigate this issue, pairs of baboons (n = 18) were exposed to different situations of TBI and PBI corresponding to an equivalent of either 5 Gy 60Co gamma irradiation (5 Gy TBI; 7.5 Gy left hemibody/2.5 right hemibody TBI; 5.55 Gy 90% PBI; 6.25 Gy 80% PBI; 10 Gy 50% PBI, 15 Gy 30% PBI) or 2.5 Gy (2.5 Gy TBI; 5 Gy 50% PBI). More than fifty parameters were evaluated before and after irradiation at several time points up to 200 days. A partial least square discriminant analysis showed a good distinction of TBI from PBI situations that were equivalent to 5 Gy. Furthermore, all the animals were pooled in two groups, TBI (n = 6) and PBI (n = 12), for comparison using a logistic regression and a non parametric statistical test. Nine plasmatic biochemical markers and most of hematological parameters turned out to discriminate between TBI and PBI animals during the prodromal phase and the manifest illness phase. The most significant biomarkers were aspartate aminotransferase, creatine kinase, lactico dehydrogenase, urea, Flt3-ligand, iron, C-reactive protein, absolute neutrophil count and neutrophil-to-lymphocyte ratio for the early period, and Flt3-ligand, iron, platelet count, hemoglobin, monocyte count, absolute neutrophil count and neutrophil-to-lymphocyte ratio for the ARS phase. These results suggest that heterogeneity could be distinguished within a range of 2.5 to 5 Gy TBI.


Pilot Study on the Homeopathic Treatment of Binge Eating in Males.
Context • Frequent binge eating is often a symptom of an underlying eating disorder, such as bulimia nervosa (BN) or binge eating disorder (BED). The role of homeopathy in the treatment of binge eating remains poorly explored. Objective • The study intended to measure the efficacy of individualized homeopathic treatment for binge eating in adult males. Design • This case study was a 9-wk pilot using an embedded, mixed-methods design. A 3-wk baseline period was followed by a 6-wk treatment period. Setting • The setting was the Homeopathic Health Clinic at the University of Johannesburg in Johannesburg, South Africa. Participants • Through purposive sampling, the research team recruited 15 Caucasian, male participants, aged 18-45 y, who were exhibiting binge eating. Intervention • Individualized homeopathic remedies were prescribed to each participant. Primary Outcome Measures • Participants were assessed by means of (1) a self-assessment calendar (SAC), recording the frequency and intensity of binging; (2) the Binge Eating Scale (BES), a psychometric evaluation of severity; and (3) case analysis evaluating changes with time. Results • Ten participants completed the study. The study found a statistically significant improvement with regard to the BES (P = .003) and the SAC (P = .006), with a large effect size, indicating that a decrease occurred in the severity and frequency of binging behavior during the study period. Conclusions • This small study showed the potential benefits of individualized homeopathic treatment of binge eating in males, decreasing both the frequency and severity of binging episodes. Follow-up studies are recommended to explore this treatment modality as a complementary therapeutic option in eating disorders characterized by binge eating.


Evaluation of effect of silymarin on granulosa cell apoptosis and follicular development in patients undergoing in vitro fertilization.
To investigate the effects of silymarin on follicular development, we enrolled 40 healthy women undergoingin vitro fertilization (IVF) due to male factor infertility in this trial. They underwent ovulation induction and on a random and blind basis, patients were assigned to receive silymarin (70 mg x 3/day) or placebo from the beginning of the induction cycle. The number and quality of oocytes retrieved were evaluated and apoptosis of > or = granolusa cells was studied. There was no significant difference between the groups for mean number of follicles 18 mm (P = 0.131), mean number of oocytes retrieved (P = 0.209) or endometrial thickness (P = 0.673). However, the proportion of total apoptosis in the study group was significantly lower than in the placebo group (P = 0.032). These data suggest that administration of silymarin in IVF patients concomitantly with gonadotropin results in reduction of granolusa cell apoptosis but does not have any effect in promotion of follicular development, oocyte retrieval or endometrial thickness.


A hexameric helicase encircles one DNA strand and excludes the other during DNA unwinding.
The bacteriophage T7 DNA helicase/primase (gene 4 protein) is a ring-like hexamer that encircles ssDNA and requires forked DNA to catalyze DNA unwinding. We report that optimal rates of unwinding of forked DNA require ssDNA tails of 55 nucleotides on the 5'-to-3' strand and 15 nucleotides on the 3'-to-5' strand. Surprisingly, streptavidin bound to a biotinylated 3'-end fully substitutes for the 3'-to-5' ssDNA tail. This suggests that excluding the 3'-to-5' DNA strand from the center of the helicase is an essential aspect of the mechanism of hexameric helicase-catalyzed DNA unwinding. We also report that streptavidin bound to a biotinylated dT within the 5'-to-3' strand of the duplexed region abolishes DNA unwinding; whereas, streptavidin bound to a biotinylated dT within the duplexed region of the other strand has no effect. These results unambiguously demonstrate that the T7 gene 4 protein is a 5'-to-3' helicase and imply that during DNA unwinding the 5'-to-3' strand transverses the center of the ring while the 3'-to-5' strand is excluded from the center of the ring. Implications for collisions between a helicase and other protein-DNA complexes are discussed.


[Cardiovascular and adrenal medullo-sympathetic reactions to acute tobacco poisoning].
We studied the effects after cigarette quicksmoking in 15 subjects on plasma catecholamines levels (epinephrine and norepinephrine), on heart rate and arterial pressure. Smoking-associated increments in mean plasma norepinephrine and epinephrine as well as in heart rate and arterial pressure were demonstrated. The lack of correlation between the increase of the adrenergic system activity and this of the hemodynamic parameters can be explained by their own different characteristics.


Flap designs for minimization of recession adjacent to maxillary anterior implant sites: a clinical study.
The purpose of this study is to present new flap designs for the prevention of postoperative gingival recession adjacent to maxillary anterior sites that received dental implants. Nine patients received 10 implants in the maxillary anterior region. Gingival morphotypes and smile lines were evaluated prior to implant placement. Gingival probing depths, clinical attachment levels, and recession were recorded at teeth adjacent to implant sites at the initial exam and 3 months after implant restoration. A minimum of 5 mm of crestal bone width was required for implant placement. The labial flaps for healed ridges and implants placed into sockets were extended to or within 1 to 3 mm beyond the alveolar crest. In two sites, transfer of the implant relationships was made to provide the patients with provisional restorations at the time of second-stage surgery. Four patients had implants placed at the time of tooth removal. In these patients, expanded polytetrafluoroethylene barrier membranes were modified, removing the outer rim of material. The inner portion of the material was placed over the implant and the flaps were sutured, leaving the center part of the material exposed. The purpose of using the barrier in this manner was to protect the clot and subsequent granulation tissue formation during the first 2 weeks of healing. The material was removed 2 weeks after surgery. Six implants were placed into edentulous sites. At second stage surgery, flaps were reflected to the alveolar crest, thereby minimizing the potential for gingival recession. Provisional restorations placed at the time of implant uncovering appeared to support the repositioned gingiva. Changes in probing depth, clinical attachment levels, and recession were not statistically or clinically significant. Results of this pilot project suggest that flap designs minimized recession at teeth next to implant sites.


Delayed matching-to-sample: A tool to assess memory and other cognitive processes in pigeons.
Delayed matching-to-sample is a versatile task that has been used to assess the nature of animal memory. Although once thought to be a relatively passive process, matching research has demonstrated considerable flexibility in how animals actively represent events in memory. But delayed matching can also demonstrate how animals fail to maintain representations in memory when they are cued that they will not be tested (directed forgetting) and how the outcome expected can serve as a choice cue. When pigeons have shown divergent retention functions following training without a delay, it has been taken as evidence of the use of a single-code/default coding strategy but in many cases an alternative account may be involved. Delayed matching has also been used to investigate equivalence learning (how animals represent stimuli when they learn that the same comparison response is correct following the presentation of two different samples) and to test for metamemory (the ability of pigeons to indicate that they understand what they know) by allowing animals to decline to be tested when they are uncertain that they remember a stimulus. How animals assess the passage of time has also been studied using the matching task. And there is evidence that when memory for the sample is impaired by a delay, rather than use the probability of being correct for choice of each of the comparison stimuli, pigeons tend to choose based on the overall sample frequency (base-rate neglect). Finally, matching has been used to identify natural color categories as well as dimensional categories in pigeons. Overall, matching to sample has provided an excellent methodology for assessing an assortment of cognitive processes in animals.


TCDD-dependent downregulation of gamma-catenin in rat liver epithelial cells (WB-F344).
TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) is the most potent tumor promoter ever tested in rodents. Although it is known that most of the effects of TCDD are mediated by binding to the aryl hydrocarbon receptor (AHR), the mechanisms leading to tumor promotion still remain to be elucidated. Loss of contact-inhibition is a characteristic hallmark in tumorigenesis. In WB-F344 cells, TCDD induces a release from contact-inhibition manifested by a 2- to 3-fold increase in DNA-synthesis and the emergence of foci when TCDD (1 nM) is given to confluent cells. We focussed our interest on potential cell membrane proteins mediating contact-inhibition in WB-F344 cells, namely E-cadherin, alpha,- beta,- and gamma-catenin (plakoglobin). Using indirect immunofluorescence, E-cadherin, alpha-, beta- and gamma-catenin were detected at cell adhesion sites in untreated, confluent cells. After TCDD-exposure, gamma-catenin was exclusively localized in the cytoplasm whereas localization of E-cadherin, alpha- and beta-catenin remained unaffected. Cytoplasmic gamma-catenin could be extracted by Triton X-100 treatment, demonstrating that gamma-catenin was no longer bound to the actin cytoskeleton. Western blot analysis showed downregulation of gamma-catenin protein levels. This effect was not blocked by pre-incubation with the selective proteasome inhibitor MG-132, indicating that proteolytical degradation of gamma-catenin by the proteasome system was not increased by TCDD. Because mRNA-levels of gamma-catenin were markedly diminished after TCDD-exposure, we conclude that transcriptional downregulation or destabilization of the mRNA contributes to the decrease in gamma-catenin protein levels in response to TCDD. Because gamma-catenin is considered to be a tumor suppressor, our findings might give more insight into the tumor promoting actions of TCDD.


[Effect of invisalign on anterior and posterior upper airway and maxillary bone changes in the treatment of high-angle skeletal Class Ⅱ malocclusion].
To investigate the effect of invisalign on anterior and posterior upper airway and maxillary bone changes in the treatment of high-angle skeletal Class II malocclusion. Thirty-seven patients with malocclusion undergoing invisible orthodontic treatment in Hospital of Stomatology of Xi'an Jiaotong University from June 2016 to June 2018 were enrolled. Before and 18 months after correction, the correction effect was evaluated. Cone-beam CT (CBCT) was performed to measure the upper airway volume and tongue position. Cephalometric examination was performed to determine the anteroposterious diameter of the upper airway and the position of hyoid bone. The pre-therapy and post-treatment facial profile images of patients and stars were graded by 0-10 numerical rating scale. SPSS 20.0 software package was used to analyze the data. After correction, oropharyngeal volume, glossopharyngeal volume and minimum cross-sectional area of upper airway significantly increased(P<0.05). Sagittal diameter of the upper airway at mandibular plane significantly increased after correction (P<0.05). After correction, ANB angle, GoGn-SN angle, OPP-SN angle, Y-axis angle, UI-SN angle, UI-PP angle, UI-AP angle, and UI-AP distance significantly decreased, SNB angle and UI- LI angle significantly increased(P<0.05). The aesthetics scores of facial profile images significantly increased after correlation(P<0.05), which was significantly lower than that of the stars(P<0.05). Invisalign can effectively correct open upper airway and malocclusion, achieving better treatment results and satisfactory facial aesthetics effect.


First report on the isolation and genotyping of Toxoplasma gondii strains from free-range chickens in the state of Mato Grosso, Midwestern Brazil.
In the present study, 51 strains of Toxoplasma gondii (T. gondii) were isolated from free-range chickens in the state of Mato Grosso, Midwestern Brazil, upon conducting bioassays in mice, and genotyped them using PCR-restriction fragment length polymorphism (PCR-RFLP) and 11 markers, including SAG1, SAG2 (5'3'SAG2 and alt. SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3. Fifty isolates were completely genotyped revealing 17 genotypes of T. gondii as follows: 12 matched using ToxoDB PCR-RFLP with the previously reported genotypes, including #6 type BrI (n = 4), #8 type BrIII (n = 7), #11 type BrII (n = 3), #14 (n = 1), #19 (n = 1), #41 (n = 1), #99 (n = 1), #109 (n = 4), #116 (n = 1), #140 (n = 2), #166 (n = 9), #190 (n = 1); and five genotypes have not been described before [#313 (n = 6), #314 (n = 1), #315 (n = 1), #316 (n = 1), #317 (n = 1)]. Moreover, mixed infections were identified in five isolates (TgCkBrMT8, TgCkBrMT9, TgCkBrMT33, TgCkBrMT38, and TgCkBrMT41). Additionally, genotype #190 was reported for the first time in chickens from Brazil. Our results corroborate with previous studies on T. gondii isolates identified in chickens from Brazil, thereby confirming their diversity, a typicality, and possibility of co-infection due to different T. gondii strains present in the country.


Dilatation of the prostatic urethra with 35 mm balloon.
A series of 54 men, 25 with acute urinary retention and 29 with cytometrically proven bladder outflow obstruction (BOO), underwent dilatation of the prostatic urethra using a 35 mm fixed diameter, low compliance balloon. In 42 patients this was performed under cystoscopic guidance and in 12 patients under fluoroscopic control. Three months following dilatation, 13/27 patients (48%) with BOO who returned for review were rendered unobstructed and 19/27 (70%) were symptomatically improved. By 6 months only 3 remained unobstructed but 15 remained symptomatically improved. Nine months after dilatation 14 patients retained symptomatic improvement but only 2 remained unobstructed. Of the 25 patients treated for acute retention only 6 voided spontaneously, 1 of these relapsing into retention at 2 months and another at 4 months. No patient was rendered unobstructed but 2 patients (who declined prostatectomy) noted an improvement in their obstructive symptoms at both 3 and 6 months. No patient developed retrograde ejaculation following dilatation. Balloon dilatation to 35 mm has no role in acute urinary retention but may have a role in younger men with BOO who wish to avoid prostatectomy and the risk of retrograde ejaculation. In these patients careful follow-up is required.


Role of Meticulous Observation: Successful Pregnancy in a 30-Year-Old Woman with Severe Pulmonary Hypertension.
Pregnancy is strongly discouraged in patients with pulmonary arterial hypertension (PAH). Herein, we report a successful delivery in a woman with PAH using a multidisciplinary approach. A 30-year-old pregnant woman with PAH was referred to us with a chief complaint of dyspnea. She was advised to terminate her pregnancy but she refused to do so despite several recommendations by healthcare professionals. She was scheduled for treatment with iloprost (brand name: Ilomedin) and heparin infusions for 3-4 days at 20-day intervals. She spent her last month of pregnancy in a hospital under close observation and received iloprost infusion. She underwent a successful cesarean section under general anesthesia at week 36 of gestation. Iloprost administration was continued for one week after delivery and was changed to bosentan after that. Meanwhile, heparin infusion was substituted by warfarin. However, treatment with bosentan led to a temporary interruption in breastfeeding. A few days later, she presented with severe dyspnea and pulmonary artery pressure of 110 mmHg. Treatment was restarted with iloprost, followed by stabilization with bosentan. A successful delivery was achieved in this situation by meticulous observation and aggressive treatment targeting PAH, along with long-term hospital stay and multidisciplinary management. Severe PAH is regarded as a contraindication to pregnancy. While physicians strongly recommend termination of pregnancy in such patients, some of them might refuse and insist on delivery of the baby. Similar pregnant cases with potential delivery are recommended to be evaluated for effective management of this condition.


Discovery Molecular Imaging Digital Ready PET/CT performance evaluation according to the NEMA NU2-2012 standard.
The aim of this study was to evaluate and benchmark the performance characteristics of the General Electric (GE) Discovery Molecular Imaging (MI) Digital Ready (DR) PET/CT. Performance evaluation against the National Electrical Manufacturers Association (NEMA) 2012 standard was performed on three GE Discovery MI DR PET/CT systems installed across different UK centres. The Discovery MI DR performance was compared with the Siemens Biograph mCT Flow, Phillips Ingenuity TF and GE Discovery 690 fully analogue PET/CT systems. In addition, as the Discovery MI DR is upgradable to the Digital MI with silicon photomultipliers, performance characteristics between analogue and digital were compared with assess potential benefits of a system upgrade. The average NEMA results across three Discovery MI DR scanners were: sensitivity 7.3 cps/kBq, spatial resolution full-width-half-maximum radial 5.5 mm, tangential 4.5 mm and axial 6 mm at 10 cm from the centre of the field-of-view, peak noise equivalent count rate 142 kcps, scatter fraction 37.1%, contrast recovery coefficients from the International Electrotechnical Commission phantom ranged from 52 to 87% for 10-37-mm diameter spheres. All three Discovery MI DR systems tested in this study exceeded the manufacturer's NEMA specification, yet variability between scanners was noted. Discovery MI DR showed similar performance to Discovery 690 and Ingenuity TF, but lower sensitivity and spatial resolution than Biograph mCT Flow. The Discovery MI DR showed lower spatial resolution and contrast recovery than the 20-cm field-of-view Digital MI.


[Neoangiogenesis in aneurysmatic autologous venous transplants].
In two patients with femoro-popliteal bypass using autogenous saphenous vein, aneurysmatic dilatation of the transplants occurred after a follow-up of 4 and 20 years. In the inner thrombus layer of the aneurysms side-branches could be shown by transfemoral angiography. A special microcorrosion cast technique for SEM-analysis was able to make a vascular network visible, which spread within the inner thrombus layer of the aneurysms. Venules were found predominantly. According to clinical and experimental results the potential of the phylogenetic older venous system is greater than the ability of the arterial system.


Laboratory markers predicting severity of acute pancreatitis.
Acute pancreatitis (AP) is an inflammatory disease of highly variable severity, ranging from mild cases with low mortality to severe cases with high mortality. Numerous biomarkers have been studied as potential early predictors of the severity of this disease so that treatment can be optimally tailored to prevent complications. We aim to present and discuss the most relevant biomarkers for early severity assessment in AP that have been studied to date. We review the current literature on biomarkers that have been used to predict the severity in AP. C-reactive protein (CRP) is still considered to be the gold standard, with a cut-off value of 150 mg/ml 48 h after disease onset. Other markers, including procalcitonin (PCT) and interleukin 6 (IL-6) have been implemented in some hospitals, but are not used on a routine basis. Most other markers, including acute phase proteins (LBP, SAA, PTX3), cytokines (Il-8, TNF-a, MIF), activation peptides of pancreatic proteases (TAP, CAPAP, PLAP), antiproteases (AAT, a2M), adhesion molecules (ICAM-1, selectins, E-cadherin) and leukocyte-derived enzymes (PA2, PMN-E) have shown some promising results but have not been routinely implemented. Furthermore, new and interesting biomarkers (Copeptin, TRX-1, Ang-2, E-2) have shown good results, but more research is needed to determine if they could play a role in the future. Various reasons why new markers for disease severity have not been adopted in daily routine include low accuracy, cumbersome laboratory techniques and high cost. Despite these difficulties, research is still very active in finding new markers to predict the severity of AP.


Voltage-Induced Wrinkle Performance in a Hydrogel by Dielectric Elastomer Actuation.
Hydrogel is a type of soft smart material and is responsive to stimuli. In the development of actuation in hydrogel, electrical actuation features a fast and universal strategy which is favored in the engineering system. Due to the difficulty in direct electrical actuation in hydrogel, in this study, an indirect actuation was attained via a dielectric elastomer. An aligned wrinkle pattern was obtained in the hydrogel upon a direct-current voltage, and it is reversible. The morphology and nonlinear mechanics of the electro-wrinkling deformation was characterized and analyzed. The optical property of the wrinkle in hydrogel was investigated, demonstrating a tunable blurring effect in optics. The electro-wrinkling performance offers a potential application with soft and tunable optical property in hydrogel-based actuators.


Scedosporium prolificans infection associated with arthritis and osteomyelitis in a horse.
Scedosporium prolificans was associated with arthritis and degenerative osteomyelitis in a 6-year-old Thoroughbred racehorse. The horse was suspected to have an inflammatory lesion of the interosseous tendon, but treatment had resulted in only a minimal response. Shortly after diagnostic arthrocentesis of the left metacarpophalangeal joint was performed, the joint became severely swollen, and radiography of the area revealed lysis of the distal end of the third metacarpal bone, the proximal sesamoid bones, and the proximal end of the proximal phalanx. The horse did not respond to treatment and was euthanatized. At necropsy, severe erosive arthritis and degenerative osteomyelitis of the left metacarpophalangeal joint were seen. Swab specimens of the ulcerated lesions of the articular cartilage were submitted for microbial culture, and Scedosporium prolificans was isolated. Scedosporium prolificans is a newly recognized opportunistic fungal pathogen of humans and animals. In humans, S prolificans typically causes focal locally invasive infections that primarily involve musculoskeletal tissues; most often, infection is a result of penetrating trauma or surgical incision. In immunocompromised patients, fatal disseminated infection can occur. The fungus is resistant to almost all currently available antimycotic agents.


Polymer types ingested by northern fulmars (Fulmarus glacialis) and southern hemisphere relatives.
Although ingestion of plastic by tubenosed seabirds has been documented regularly, identification of the polymer composition of these plastics has rarely been described. Polymer assessment may assist in identifying sources and may indicate risks from additives occurring in specific types of polymers. Using known test materials, two identification methods Fourier transform infrared spectroscopy and near infrared spectroscopy (FTIR and NIR) were compared. Although both methods were found to be similarly suitable for identification of plastic polymers, a significant difference was observed in identification of natural materials. FTIR frequently misclassified natural materials as being a synthetic polymer. Within our results, an 80% match score threshold functioned best to distinguish between natural items and synthetics. Using NIR, the historical variability of plastics ingested by northern fulmars (Fulmarus glacialis) from the Dutch sector of the North Sea was analysed for three time periods since the 1980s. For the more recent decade, variability between fulmars from different regions in the northeast Atlantic was investigated. Regional variation was further explored by analysing plastics obtained from the stomachs of southern hemisphere relatives of the fulmar (southern fulmar, cape petrel, snow petrel) and Wilson's storm petrel. Results show that proportional abundance of polymer types in these seabirds is closely related to the plastic categories that they ingest (e.g. pellets, foam, fragments). The uptake of different plastic categories and related polymer types most likely reflects spatial and temporal variations in availability rather than ingestion preferences of the birds.


Autologous stem cell transplantation and multiple myeloma cancer stem cells.
It is well established that high-dose therapy (HDT) combined with autologous stem cell transplantation (ASCT) produces superior response rates and progression-free survival compared with conventional chemotherapy in patients with multiple myeloma (MM). Accordingly, MM currently represents the most common indication for ASCT. Despite these clinical improvements, the impact of ASCT on overall survival is unclear because the vast majority of patients eventually experience disease relapse and progression. The continual risk of relapse suggests that malignant cells resistant to HDT possess the clonogenic growth potential to mediate tumor regrowth, and in several diseases cancer stem cells (CSCs) have been identified that are both highly tumorigenic and resistant to standard anticancer approaches. Putative CSCs have been identified in MM, and their characterization may lead to the development of novel maintenance strategies that inhibit the production of new tumor cells, prevent disease relapse, and improve overall survival.


Survey of the vaia storm deposits in the tegnas catchment (Dolomites, Italy): Field data and evidence of sediment-water flow types.
Brenna et al. [1] developed a survey protocol to collect evidence aimed at classifying flood deposits on the basis of the flow type that mobilized and deposited sediment. Such a survey protocol was adopted to characterize the flood deposits in a mountain catchment of the Dolomites (the Tegnas Torrent and its tributaries; drainage area of 51 km2) after a high-magnitude hydrological event that occurred in October 2018 (the so-called "Vaia Storm"). In this article, we present the field data collected at thirty-two survey sites considering the geomorphological and sedimentological characteristics of the analysed sedimentary products and their effects on the vegetation. Data on the characteristics of the flood deposits have enabled recognizing the transport mechanisms that occurred during the Vaia Storm along the stream network [1]. Future applications of the survey protocol adopted in this study could compare and integrate the collected data with those presented in detail in this article.


Bioreactor and scaffold design for the mechanical stimulation of anterior cruciate ligament grafts.
Various studies have shown that physical stimuli modulate cell function and this has motivated the development of a bioreactor to engineer tissues in vitro by exposing them to mechanical loads. Here, we present a bioreactor for the physical stimulation of anterior cruciate ligament (ACL) grafts, whereby complex multi-dimensional strain can be applied to the matrices. Influences from environmental conditions to the behavior of different cells on our custom-made silk scaffold can be investigated since the design of the bioreactor allows controlling these parameters precisely. With the braided design of the presented silk scaffold we achieve maximum loads and stiffness values matching those of the human ACL. Thus, the existent degummed and wet silk scaffolds absorb maximum loads of 2030±109 N with stiffness values of 336±40 N/mm.


In vivo efficacy of artesunate-amodiaquine and artemether-lumefantrine for the treatment of uncomplicated falciparum malaria: an open-randomized, non-inferiority clinical trial in South Kivu, Democratic Republic of Congo.
Between 2009 and 2012, malaria cases diagnosed in a Médecins sans Frontières programme have increased fivefold in Baraka, South Kivu, Democratic Republic of the Congo (DRC). The cause of this increase is not known. An in vivo drug efficacy trial was conducted to determine whether increased treatment failure rates may have contributed to the apparent increase in malaria diagnoses. In an open-randomized non-inferiority trial, the efficacy of artesunate-amodiaquine (ASAQ) was compared to artemether-lumefantrine (AL) for the treatment of uncomplicated falciparum malaria in 288 children aged 6-59 months. Included children had directly supervised treatment and were then followed for 42 days with weekly clinical and parasitological evaluations. The blood samples of children found to have recurring parasitaemia within 42 days were checked by PCR to confirm whether or not this was due to reinfection or recrudescence (i.e. treatment failure). Out of 873 children screened, 585 (67 %) were excluded and 288 children were randomized to either ASAQ or AL. At day 42 of follow up, the treatment efficacy of ASAQ was 78 % before and 95 % after PCR correction for re-infections. In the AL-arm, treatment efficacy was 84 % before and 99.0 % after PCR correction. Treatment efficacy after PCR correction was within the margin of non-inferiority as set for this study. Fewer children in the AL arm reported adverse reactions. ASAQ is still effective as a treatment for uncomplicated malaria in Baraka, South Kivu, DRC. In this region, AL may have higher efficacy but additional trials are required to draw this conclusion with confidence. The high re-infection rate in South-Kivu indicates intense malaria transmission. Trial registration NCT02741024.


[Antimicrobial susceptibilities of organisms isolated from patients with urinary tract infections in 2002].
Using the agar dilution method, the antibacterial activity of 18 antibiotics inclusive of 4 carbapenems were investigated against 101 strains of urinary pathogens isolated from patients with urinary tract infections who visited the Department of Urology at Kagoshima University Hospital, between January and December 2002. 4 strains of Staphylococcus aureus, 3 strains of Staphylococcus spp. (exclusive of S. aureus), 14 strains of Enterococcus faecalis, 3 strains of Enterococcus spp. (exclusive of E. faecalis), 41 strains of Escherichia coli, 21 strains of Enterobacteriaceae (exclusive of E. coli), 12 strains of Pseudomonas aeruginosa and 3 strains of glucose-nonfermentative Gram-negative rods (exclusive of P. aeruginosa) were examined. 1. Against clinical isolates of Gram-positive bacteria, vancomycin and teicoplanin were active. Additionally, arbekacin was active against S. aureus clinical isolates and ampicillin was active against E. faecalis clinical isolates. Carbapenems were active against clinical isolates of Gram-positive bacteria, except for multi-drug resistant strains of Gram-positive bacteria, such as methicillin-resistant S. aureus. 2. As for clinical isolates of Gram-negative bacteria, meropenem was most active against Enterobacteriaceae among 13 antibiotics tested. Against P. aeruginosa clinical isolates, MIC90 of meropenem was the lowest among 13 antibiotics tested. In addition, resistant rate of meropenem and biapenem against P. aeruginosa clinical isolates was lower than those of the other carbapenems tested. 3. As main urinary pathogens showed no remarkable increase in resistance to carbapenems, it can be stated that carbapenems retain their position as the drug of first choice for severe infection.


Exosomes as New Players in HIV Pathogenesis - New Data from the IAS 2017.
Exosomes are nanovesicles that can be released into the extracellular medium by different cell types and are considered an important system of intercellular communication. In some instances, on secretion, exosomes break down and release their content into the extracellular space. Alternatively, intact exosomes can interact with other cells and discharge their content directly into the target cell cytoplasm. Exosomes are rich in endosome-associated proteins (i.e., the tetraspanin family) but also carry different molecules in their lumen including proteins, RNAs (i.e., microRNAs), and pathogenderived cargo. Indeed, the different biological functions of exosomes might depend on their cargo components. Several recent publications have identified exosomes as new players in the pathogenesis of HIV infection. This is an emerging field of growing interest that deserved a satellite symposium at the 9th IAS Conference held in Paris in July 2017, sponsored by the International Society for Extracellular Vesicles (EVs). Although the role of exosomes in HIV infection is not yet clarified, accumulating data suggest that exosomes may act at different levels of HIV pathogenesis by modulating immune responses, infectivity, or even by activating the latent viral reservoir. However, there are still methodological limitations that need to be resolved to advance our understanding of the role of exosomes in HIV infection. One particular limitation is to define an accurate and reproducible procedure for their isolation. A new strategy to separate exosomes from HIV-1-infected cells was proposed at this symposium. Martin-Jaular and colleagues (Institut Curie, France) used velocity centrifugation gradients and antibody labeling of specific endosome-associated protein markers (i.e., CD45+ and AChE+) to isolate pure exosomes from complex preparations of EVs. Separately, Arakelyan and colleagues (NIH, USA) used flow cytometry coupled with magnetic nanoparticles to characterize the antigenic composition of EVs and demonstrate that EVs carrying HIV envelope proteins facilitated HIV infection. This is an interesting observation that could lead to the discovery of new antiviral drugs targeting EVs that contain HIV-Env. Exosomes might also have an effect on the viral reservoir. Kashanchi et al. (George Mason University, USA) presented interesting results demonstrating how exosomes from HIV-uninfected cells could reactivate latent HIV-1 in infected cells. They proposed a mechanism in which exosomes might increase RNA polymerase II loading onto the HIV-1 promoter in the infected cells, thereby facilitating transcription and leading to an increase of cellular activation. These presentations represent just a small piece of the growing body of research focused on the role of exosomes in HIV infection. We stand at the beginning of a new source of knowledge of HIV pathogenesis that may provide novel strategies to control HIV infectivity, regulate HIV-reactive immune responses, and act against the HIV reservoir. The impact of exosomes on HIV infection at all these levels should be considered in ongoing and future approaches to achieve HIV remission and cure.


Response biases as a confound to expressed burden among spousal caregivers of suspected dementia patients.
Burden among family caregivers has yet to be adequately assessed relative to social desirability response sets. This study examined the impact of two different response biases relative to expressed burden among spousal caregivers of suspected dementia patients. In addition to individual social desirability, it was hypothesized that a distinct social desirability construct exists as a function of one's perception of self within relationships. As predicted, a significant inverse association was apparent between expressed burden and this systemic response bias (as measured by the Edmonds Marital Conventionality Scale), whereas this association was not apparent with a measure of individual social desirability (the Marlowe-Crowne Social Desirability Scale). This finding suggests that systemic social desirability may exist as a significant response bias.


Preparative linear ion trap mass spectrometer for separation and collection of purified proteins and peptides in arrays using ion soft landing.
A preparative mass spectrometer for microarray fabrication is reported. The instrument includes an atmospheric pressure ionization source, a linear ion trap mass analyzer, an ion collection surface positioning system, and a surface loading chamber with independent vacuum pumping. It was designed for the production of protein arrays using the ion soft-landing technique to collect ions on a surface after separation by mass/charge ratio. Small microarrays have been prepared by isolating and soft landing individual protein or peptide ions after electrospray ionization of mixtures. The composition and purity of the separated materials has been confirmed using independent external mass spectrometric analysis of rinse solutions of the collected spots, either by the new method of electrosonic spray ionization MS or by nanospray ionization MS. The ability to retain bioactivity in the mass-selected and collected biomolecules has been demonstrated in particular cases. The reported instrument has also been characterized as an analytical mass spectrometer.


Bimanual Fundamentals: Validation of a New Curriculum for Virtual Reality Training of Laparoscopic Skills.
Background. To determine face and construct validity for the new Bimanual Fundamentals curriculum for the Simendo® Virtual Reality Laparoscopy Simulator and prove its efficiency as a training and objective assessment tool for surgical resident's advanced psychomotor skills. Methods. 49 participants were recruited: 17 medical students (novices), 15 residents (intermediates), and 17 medical specialists (experts) in the field of gynecology, general surgery, and urology in 3 tertiary medical centers in the Netherlands. All participants performed the 5 exercises of the curriculum for 3 consecutive times on the simulator. Intermediates and experts filled in a questionnaire afterward, regarding the reality of the simulator and training goals of each exercise. Statistical analysis of performance was performed between novices, intermediates, and experts. Parameters such as task time, collisions/displacements, and path length right and left were compared between groups. Additionally, a total performance score was calculated for each participant. Results. Face validity scores regarding realism and training goals were overall positive (median scores of 4 on a 5-point Likert scale). Participants felt that the curriculum was a useful addition to the previous curricula and the used simulator would fit in their residency programs. Construct validity results showed significant differences on the great majority of measured parameters between groups. The simulator is able to differentiate between performers with different levels of laparoscopic experience. Conclusions. Face and construct validity for the new Bimanual Fundamental curriculum for the Simendo virtual reality simulator could be established. The curriculum is suitable to use in resident's training programs to improve and maintain advanced psychomotor skills.


Contractile response of the isolated trabecular meshwork and ciliary muscle to cholinergic and adrenergic agents.
To characterize the contractile properties of isolated trabecular meshwork strips, we measured the effect of various cholinergic and adrenergic substances on the contractility of trabecular meshwork (TM) strips in comparison with the effects on ciliary muscle (CM) strips. Using an electromagnetic force length transducer we performed measurements of isometric tension on isolated bovine TM and CM strips. Isolated strips were contracted by cholinergic agonists, the relative potency being carbachol > pilocarpine > acetylcholine. The half-maximal effective concentration was 2 x 10(-7) mol l(-1) for carbachol and 2 x 10(-6) mol l(-1) for pilocarpine. To characterize muscarinic receptors, we tested selective antagonists for M1 (pirenzepine) and M3 (4-DAMP). Pharmacologically, the functional muscarinic receptors are of the M3 subtype in TM as well as CM. The M1 subtype seems to be less important. The alpha 1-agonist phenylephrine was more effective in inducing contractions in TM than in CM. The alpha 2-agonist brimonidine induced contractions only in TM. In precontracted tissues the beta-agonist isoproterenol induced a relaxation in both tissues. This relaxation could be inhibited by metipranolol. Epinephrine (or dipivefrin) induced small contractions in TM and CM, which became more prominent, especially in TM, when the beta-adrenoreceptors were inhibited by metipranolol. The data indicate the presence of functional muscarinic, alpha-adrenergic, and beta-adrenergic receptors in bovine TM and CM. The contractile properties of TM and CM are differently modulated by the various drugs. Cholinergic and alpha-adrenergic agonists induced contraction, whereas beta-agonists induced relaxation.


Parents' perceptions, attitudes and acceptability of treatment of childhood malaria with artemisinin combination therapies in ghana.
There is little information on sociocultural and contextual factors that may influence attitudes of patients to new treatments, such as artemisinin combination therapies (ACT). Semi-structured questionnaires and focus group discussions were used to assess views of parents of children with uncomplicated malaria treated with ACT in a low socio-economic area in Accra, Ghana. The majority of parents reported a favourable experience, in terms of perceived i) rapidity of symptom resolution, compared to their previous experience of other therapies for childhood malaria, or ii) when their experience was compared that of parents of children treated with monotherapy. The parents of children treated with ACT were more willing to pay for the treatment, or adhere to the full treatment course. The explanations given for adherence were consistent with conventional biomedical explanations. Although care-seeking practices for childhood malaria were considered appropriate, perceived or real barriers to accessible health care were also important factors in the decision to seek treatment. Household dynamics and perceived inequities at the care-provider-patient interface were identified as having potential negative impact on care-seeking practices and adherence. Health education messages aimed at improving the response to childhood febrile illness should include other strategic stakeholders, such as decision-makers at the household level. The effectiveness and implementation success of the ACT policy could be enhanced by highlighting and reinforcing messages intrinsic to these regimens. Integrating the views of caretakers during the clinical encounter was validated as an empowerment tool that could aid in the appropriate responses to childhood illness.


[Surveillance of human soil-transmitted nematodiasis in Jurong City from 2016 to 2020].
To investigate the prevalence of soil-transmitted nematode human infections in Jurong City from 2016 to 2020, so as to provide the scientific evidence for formulating the control strategy. During the period from 2016 to 2020, the permanent residents at ages of over 3 years living in Jurong City were selected as the study subjects. Stool samples were collected for the detection of soil-transmitted nematode eggs using the modified Kato-Katz thick smear method (two detections for one stool sample), and the species of hookworm was identified in stool-positive stool samples using the culture method. The prevalence and intensity of soil-transmitted nematode infections were calculated, and the change of the infection prevalence among years was examined using the Cochran-Armitage test for trend. A total of 10 011 people-time populations were detected for soil-transmitted nematode infections in Jurong City from 2016 to 2020, and 56 egg-positives were identified, with mean prevalence of 0.56%. The prevalence of soil-transmitted nematode human infections appeared a tendency towards a decline year by year in Jurong City (χ2trend = 5.15, P < 0.01). The mean prevalence of hookworm, Ascaris lumbricoides and Trichuris trichiura infections was 0.44%, 0.11% and 0.20% in Jurong City from 2016 to 2020, respectively, and individuals with hookworm infections accounted for 78.57% of all cases with soil-transmitted nematode infections. Single parasite (98.21%) and mild infection were pre-dominant in individuals with soil-transmitted nematode infections, and no multiple infections were seen after 2016. The prevalence of human soil-transmitted nematodiasis is low in Jurong City. Based on reinforcement of soil-transmitted nematodiasis surveillance, an increase in the health education investment is required to consolidate the control achievements.


The Effect of Breastfeeding on Intelligence Quotient and Social Intelligence Among Seven- to Nine-Year-Old Girls: A Pilot Study.
Breastfeeding is an optimal infant feeding method that provides adequate nutrients, achieves healthy growth and development, and enhances the health status of both infants and mothers. Breast milk contains a variety of substances that might positively affect cognition and the development of children's psychomotor abilities. This study aimed to examine the variations in intelligence quotient (IQ), social intelligence (SI), and body mass index (BMI) among 7- to 9-year-old girls who were exclusively breastfed, exclusively bottle-fed, or mixed-fed during their first 6 months of life. This study involved 111 healthy girls, aged 7 to 9 years, who were recruited from nine government and private schools in Dammam, Kingdom of Saudi Arabia. Raven's Coloured Progressive Matrices were used to measure the participants' IQs, and the Vineland Social Maturity Assessment was used to measure their SI through individual interviews. Anthropometric measurements were obtained using standard methods. The breastfed group showed a greater number of above-average IQ test scores (35 vs. 23%; P = 0.479) and better SI scores (78 vs. 55%; P = 0.066) compared with the bottle-fed group. The number of girls with normal BMIs was significantly higher in the breastfed group than in the bottle-fed (68 vs. 41%; P = 0.045) or mixed-fed groups. Exclusively breastfed girls had higher IQ and SI results compared with bottle-fed girls. However, unlike the BMI differences, these results were not statistically significant. This study provides fundamental observational data and can be further modified for use on a larger national-scale level.


Creating a safe environment for children in daycare.
Injuries represent the leading cause of death for children older than age one. Likewise, some 22 million children are injured each year, with more than 1 million requiring medical treatment. More than 40% of children currently receive full-time or part-time day care, but little research has examined the status of injuries to children in daycare. This paper provides information on the nature and extent of injuries to children, and contributing factors responsible for those injuries. One case study of research on injuries in Minneapolis, Minn., daycare centers is discussed. Recommendations for action are offered.


[Microalbuminuria. Signification and value].
The term microalbuminuria means a slightly elevated urinary albumin level. It indicates an abnormal passage of plasma albumin into urine through the glomerular filter. Its prognostic significance was first established in diabetics: in insulin-dependent diabetics microalbuminuria predicts a renal disease, while in non-insulin-dependent diabetics it predicts an excessive cardiovascular mortality. Recently, microalbuminuria has been shown to predict cardiovascular morbidity and mortality in the general population, especially in elderly subjects. Pathogenetic mechanisms common to both atherosclerosis and renal failure may account for the predictive value of microalbuminuria in the above-mentioned conditions.


The role of prostaglandins in NSAID induced renal dysfunction.
Renal prostaglandins (PG) play an important role in regulating renal hemodynamics and excretory function in 2 conditions (1) which effective arterial blood volume is reduced and (2) chronic renal failure is reduced. The use of nonsteroidal antiinflammatory drugs (NSAID) in patients with these conditions can produce a significant decline in renal function, including decreased glomerular filtration rate and solute and water excretion, by inhibiting renal cyclooxygenase, and thus, PG production. Results of preliminary investigation with the PGE1 analog misoprostol suggest that exogenous PG analogs may be effective in counteracting the effects of NSAID in such settings. Further clinical studies will be needed to establish the role of PG in this setting.


Truncation effects in superdiffusive front propagation with Lévy flights.
A numerical and analytical study of the role of exponentially truncated Lévy flights in the superdiffusive propagation of fronts in reaction-diffusion systems is presented. The study is based on a variation of the Fisher-Kolmogorov equation where the diffusion operator is replaced by a lambda -truncated fractional derivative of order alpha , where 1lambda is the characteristic truncation length scale. For lambda=0 there is no truncation, and fronts exhibit exponential acceleration and algebraically decaying tails. It is shown that for lambda not equal0 this phenomenology prevails in the intermediate asymptotic regime (chit);{1alpha}x1lambda where chi is the diffusion constant. Outside the intermediate asymptotic regime, i.e., for x>1lambda , the tail of the front exhibits the tempered decay varphi approximately e;{-lambdax}x;{(1+alpha)} , the acceleration is transient, and the front velocity v_{L} approaches the terminal speed v_{*}=(gamma-lambda;{alpha}chi)lambda as t-->infinity , where it is assumed that gamma>lambda;{alpha}chi with gamma denoting the growth rate of the reaction kinetics. However, the convergence of this process is algebraic, v_{L} approximately v_{*}-alpha(lambdat) , which is very slow compared to the exponential convergence observed in the diffusive (Gaussian) case. An overtruncated regime in which the characteristic truncation length scale is shorter than the length scale of the decay of the initial condition, 1nu , is also identified. In this extreme regime, fronts exhibit exponential tails, varphi approximately e;{-nux} , and move at the constant velocity v=(gamma-lambda;{alpha}chi)nu .


Myosin X Interaction with KIF13B, a Crucial Pathway for Netrin-1-Induced Axonal Development.
Myosin X (Myo X) transports cargos to the tips of filopodia for cell adhesion, migration, and neuronal axon guidance. Deleted in Colorectal Cancer (DCC) is one of the Myo X cargos that is essential for Netrin-1-regulated axon pathfinding. The function of Myo X in axon development in vivo and the underlying mechanisms remain elusive. Here, we provide evidence for the role of Myo X in Netrin-1-DCC-regulated axon development in developing mouse neocortex. The knockout (KO) or knockdown (KD) of Myo X in cortical neurons of embryonic mouse brain impairs axon initiation and contralateral branching/targeting. Similar axon deficits are detected in Netrin-1-KO or DCC-KD cortical neurons. Further proteomic analysis of Myo X binding proteins identifies KIF13B (a kinesin family motor protein). The Myo X interaction with KIF13B is induced by Netrin-1. Netrin-1 promotes anterograde transportation of Myo X into axons in a KIF13B-dependent manner. KIF13B-KD cortical neurons exhibit similar axon deficits. Together, these results reveal Myo X-KIF13B as a critical pathway for Netrin-1-promoted axon initiation and branching/targeting.SIGNIFICANCE STATEMENT Netrin-1 increases Myosin X (Myo X) interaction with KIF13B, and thus promotes axonal delivery of Myo X and axon initiation and contralateral branching in developing cerebral neurons, revealing unrecognized functions and mechanisms underlying Netrin-1 regulation of axon development.


Glucocorticoids stimulate rabbit proximal convoluted tubule acidification.
Glucocorticoids have an important role in renal acidification; however, a direct effect of glucocorticoids on proximal convoluted tubule (PCT) acidification has not been directly demonstrated. In the present in vitro microperfusion study PCT from animals receiving dexamethasone (600 micrograms/kg twice daily for 2 d and 2 h before killing) had a significantly higher rate of bicarbonate absorption than did controls (92.0 +/- 13.3 vs 59.9 +/- 3.2 pmol/mm.min, P < 0.01). To examine if glucocorticoids had a direct epithelial action, dexamethasone was added to the bath of PCT perfused in vitro. After 3 h of incubation in paired experiments 10(-6) M and 10(-5) M dexamethasone resulted in an approximately 30% stimulation in the rate of bicarbonate absorption. 10(-7) M dexamethasone and 10(-6) M aldosterone had no effect on bicarbonate absorption. The stimulation of acidification by 10(-5) M dexamethasone was blocked by actinomycin D and cycloheximide. These data are consistent with a direct effect of glucocorticoids on PCT acidification, and this effect is dependent upon protein synthesis.