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About Us
We're a Full Service Florist offering exceptional value "field fresh quality for less".
Whatever the Occasion Ready-to-go or custom floral designs to suit any occasion, including personal special occasions, corporate needs, funerals, and weddings. Our talented floral designers create stunning wedding packages, with free consultations and on-site quotes to meet your unique dreams.
More Choice - Gorgeous Fresh Floral Designs in our Spacious Walk-in Coolers Choose from our vast display of European hand-tied bouquets and themed arrangements for all occasions, with a great selection of exotic tropical's. They're conveniently ready-to-go to minimize your wait time.
Superior Quality - Fresher, Longer Lasting Flowers in Your Home. Our streamlined supply chain reduces handling and time in transit. Continuous refrigeration during transit, and our walk-in coolers, help preserve this freshness and quality.
More Affordable Prices for Exceptional Value. Savings through wholesale volume discounts and fewer "middlemen" allow us to keep our prices lower than other florists.
More Convenient, Meeting Your Lifestyle Needs We're open 7 days a week, with free parking directly in front. On-line 24/7.
Worldwide Delivery and Same Day Local Delivery We provide same day delivery in our Local Delivery Areas in London, Mississauga, Oakville, Burlington, St. Thomas, Strathroy, Stratford, Kitchener, Brantford, and surrounding areas. We can meet your floral delivery needs worldwide through the Teleflora and FTD network of retail florists.
100% Unconditional Satisfaction Guarantee. Your assurance of superior product quality and customer service.
No Contact Delivery Policy
To protect everyone's health and safety we have instituted a no contact delivery policy. The store will call the recipient prior to delivery to ensure someone is available to accept. The driver will ring the doorbell or knock on the door, leave the flowers on the porch and then step back to a safe distance to ensure that the recipient receives their delivery.
Ontario's Fastest Growing Florist Chain
Over the past 20 years, Forest of Flowers has become London's most popular florist. In 2012, London Free Press readers voted us London's best Florist, and we have held the title since. We are committed to becoming your favourite florist in all of our current locations.
Franchise Opportunities for a Fresh New Start
We offer franchise opportunities across Ontario. Start building your future today – join the growing Forest of Flowers success story.


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19th Annual Fall Scientific Meeting
November 21 – 24, 2013
InterContinental New Orleans
New Orleans, Louisiana
General Meeting Information
Registration/Information Desk Hours:
Thursday, November 21, 2013: 8:00 a.m. – 5:00 p.m.
Friday, November 22, 2013: 6:30 a.m. – 5:00 p.m.
Saturday, November 23, 2013: 7:00 a.m. – 5:00 p.m.
Sunday, November 24, 2013: 7:30 a.m. – 10:00 a.m.
Exhibit Hall Hours:
Thursday, November 21, 2013: 12:30 p.m. – 7:00 p.m.
Friday, November 22, 2013: 7:00 a.m. – 4:00 p.m.
Saturday, November 23, 2013: 10:00 a.m. – 3:00 p.m.
Evening Functions
One ticket to each evening function is included in attendee and spouse registration fees.
Welcome Reception
Date: Thursday, November 21, 2013
Time: 5:00 p.m. – 7:00 p.m.
Location: La Salle Ballroom, Exhibit Hall
Attire: Business casual
Cost: One ticket is included in attendee and spouse registration fees; additional tickets are $70.00 USD
Welcome to the SMSNA 2013 Fall Scientific Meeting! Catch up with friends and colleagues, and meet with exhibitors following the first day of the scientific program.
New Orleans Themed Night
Date: Friday, November 22, 2013
Time: 7:00 p.m. – 10:00 p.m.
Location: Special Event Venue *Meet at the InterContinental ground level, at the "Blue Dogs" no later than 7:00 p.m.
Attire: Business casual
Cost: One ticket is included in attendee and spouse registration fees; additional tickets are $70.00 USD
The laid back and relaxed culture of New Orleans makes it a perfect location for a very special Themed Night in the "Big Easy". Join SMSNA this evening and let the good times roll!
Annual Banquet
Date: Saturday, November 23, 2013
Time: 7:00 p.m. – 10:00 p.m.
Location: La Salle Ballroom
Attire: Business
Cost: One ticket is included in attendee and spouse registration fees; additional tickets are $70.00 USD
Join SMSNA for a fantastic evening full fabulous cuisine, New Orleans fun and live music featuring Deacon John. For many decades Deacon John has symbolized the ultimate in dance music. Deacon, with his band Deacon John & the Ivories, was the first rock 'n' roll musician to play with the New Orleans Symphony in 1970. A t the: invitation of President Bush, he played at the Congressional Ball at the White House in 2005 and in 2007, he was chosen to sing for the historic inauguration of Louisiana Governor Bobby Jindal. Don't miss this special event!
Hotel Information
InterContinental New Orleans
444 St. Charles Avenue
New Orleans, LA 70130
Main: (504) 525-5566
Fax: ( 504) 526-7310
Website: http://www.intercontinental.com/neworleans
Room Rate
Superior Room: $189.00 (plus applicable taxes)
Deluxe Room: $219.00 (plus applicable taxes)
Club Level Room: $249 (plus applicable taxes)
SMSNA has negotiated a discounted rate of $189.00 plus tax (currently 13%) for a superior room at the InterContinental New Orleans. Additional charges for people over 17 years of age are $30.00 per person per night with a maximum of four people per room, including minors. Deluxe rooms with a balcony overlooking the interior courtyard are available at a rate of $219.00 plus tax and club level rooms which feature a larger room on a key access only floor with concierge lounge privileges are available at a rate of $249.00 plus tax.
Hotel Deadline
October 25, 2013
The deadline to receive the SMSNA group rate is October 25, 2013. SMSNA encourages you to make your reservation early, as the hotel and discount block may sellout before this date. After this date, reservations will be accepted based on availability and higher rates may apply.
Attendees are responsible for making their reservations by calling the hotel at (888) IC HOTELS or visiting the website at https://resweb.passkey.com/go/sexualmedicinesociety. Please reference the SMSNA to receive the discounted rate.
Cancellation Policy
Reservations cancelled after 72 hours prior to arrival, will be charged one night room and tax penalty.
Travel & Transportation
Airport Information
Louis Armstrong New Orleans International Airport is approximately 15 miles from the InterContinental New Orleans or 22 minutes by car.
Airport Shuttle
Shuttle service is available from the airport to the hotels in the CBD for $20.00 (per person, one-way) or $38.00 (per person, round-trip). Three bags per person. Call (866) 596-2699 or (504) 522-3500 for more details or to make a reservation. Advance reservations are required 48 hours prior to travel for all ADA accessible transfers. Please call in advance of your travel date for the specially-equipped shuttle to be reserved. For group reservations of 50 or more people please dial (866) 596-2699. Ticket booths are located on the lower level in the baggage claim area. www.airportshuttleneworleans.com.
Taxi Cab Services
Several taxi companies operate at the Louis Armstrong New Orleans International Airport.
American United Cab: (504) 522-9771
Checker Taxi: (504) 684-4440
Flash Cab: (504) 299-0386
Taxi pickup is on the lower level, outside the baggage claim area. Taxi rates for a one-way transfer to the hotel cost approximately $36.00. Taxis are required to offer a credit card payment option.
Rental Car Information
Avis Rent-A-Car is the official rental car company for the SMSNA 19 th Annual Fall Scientific Meeting. For reservations, please call (800) 331-1600, and use the code " J901055" to receive the discounted rates.
The InterContinental New Orleans offers valet parking for $36.00 per day. Please note that rates are subject to change.
About New Orleans, LA
A great way to get excited about their upcoming trip and enjoy interviews, rebroadcasts, pictures and recommendations on all the great things to do in New Orleans!
A local "online concierge" to answer any questions about the city…all at no charge to anyone!
Online request for dining reservations & interactive map with dozens of popular restaurants!
A printer friendly map giving the user the option to print the entire list of restaurants along with the map or only print the specific areas of town they desire! http://www.neworleansrestaurants.com/map/print.php
Discount coupons and special offers to print before they leave home!
Additional Registration Information
Registration Form
The online registration form is available here.
Registration Fee Includes
- Entrance to scientific sessions
- Breakfasts
- One ticket to the Welcome Reception
- One ticket to the New Orleans Parade to Bourbon Vieux
- One ticket to the Annual Banquet
- Program Materials
Spouse/Guest Registration Fee Includes
- One ticket to the Welcome Reception
- One ticket to the New Orleans Parade to Bourbon Vieux
- One ticket to the Annual Banquet
Early Registration Deadline
Meeting registration forms must be received by the SMSNA Executive Office by 10/25/13 to receive the discounted early registration rate. All forms received after this deadline will be assessed the regular registration fee.
Registration Cancellation & Refund Policy
Registration refund requests must be submitted in writing to the SMSNA Executive Office by 10/21/13. All refund requests will be subject to a $100 processing fee. No refunds will be made after 10/21/13.
Special Needs
For accommodations on the basis of disability or special dietary needs, please call +1 (952) 683 1917.
Please feel free to contact the SMSNA Executive Office by:
PO Box 1233
Lakeville, MN 55044
Phone: +1 (952) 683 1917
Email: firstname.lastname@example.com

subset: commoncrawl


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Recent Posts
- 153 Hail Mary's and 153 Hilda
- A Desert Place (a Different View)
- Abomination of Desolation: Part one
- An Holy Kiss
- Blue Skies
- Boomerangs and Dogs Without Legs
- Eye of a Needle more posts by Merangue
- Folly of the Synoptic Gospels
- How is it That Ye Do Not Understand?
- I Am the True Vine
- In The Garden of Gethsemane
- Indulgences and the Parable of Usury
- Japan Quakes after Confirmation of the Neocatechumenal Way
- Judge Not, That You Be Not Judged
- Keys of the Kingdom of Heaven
- Mary's Spikenard and Luke's Parable
- Merangue's Blog
- News that matters (Exposing fake and falsehood)
- Now is the Judgement of This World
- Prophecies of the Betrayal of the Son of Man
- Prophecy of Esaias
- Rev. David Wilkerson and Evangelicalism
- Salute No Man
- Seven Mountains of Revelation
- Simon, Simon – Who is Simon's Son?
- Simon, Simon – Who is Simon's Son? Part Two
- Sour Wine
- Tell No Man
- The Beasts of Revelation
- The Bohemian Grove, the Vatican and the NWO
- THE Drawbridge A REALLY GREAT SITE
- The Drawbridge
- The Fig Tree – Blessed are the Barren
- The Fig Tree – Blessed are the Barren
- The Fruits of the Vatican
- The Gospel of Luke – A Parody
- The Gospel of Luke – A Parody
- The Grace of Anathema Maranatha
- The Image of the Beast
- The Moral Authority of the Pope
- The Mother of Jesus Christ: SHALOM!
- The Mount of the Congregation
- The Mount of the Congregation
- The Parable of Faith – Part two
- The Parable of Faith – Part two
- The Seventh and the Eighth Pope of Revelation- Benedict XVI
- The Seventh and the Eighth Pope of Revelation- Benedict XVI
- The Son of Simon Part Three
- The Son of Simon Part Three
- The Stone Which the Builders Rejected-PART 1
- The Stones of Amen
- The Stones of Amen
- The Synoptic "Q Source"
- The Synoptic Gospels and The Dome of the Rock
- The Temptation of Jesus Christ
- The Temptation of Jesus Christ
- The Vatican-The Family Business-The Vatican City
Tag Archives: Former Texas First Lady Nellie Connally
Jackie Kennedy and Nellie Connally PART 2 Nancy De Graffenried
Warren Commission Testimony of Mrs John F. Kennedy Mrs. KENNEDY. ……And then–do you want me to tell you what happened? Mr. RANKIN. Yes; if you would, please. Mrs. KENNEDY. You know, there is always noise in a motorcade … Continue reading
Posted in 1963, Abomination of Desolation, Acts, Assassination of President Kennedy, Books about the assassination of President Kennedy, Books about the Kennedys, C-span, CONNALLY, DALLAS TEXAS, First Lady Nellie Connally, Former Texas First Lady Nellie Connally, GARY MACK, House of Representatives, Jackie Kennedy, Jackie O, Jackie Onassis, JFK, JFK ASSASSINATION, John F. Kennedy assassination, KENNEDY, Kennedy assassination, Life Magazine, Nancy, National Politics, NELLIE CONNALLY, North Carolina, President John F. Kennedy, President Kennedy and the Secret Service, PRESIDENT KENNEDY'S ARRIVAL AT LOVE FIELD IN DALLAS ON 11/22/63, RUFUS YOUNGBLOOD, Secret Service, Texas Politics, The Assassination, The Assassination of President Kennedy, The Beast, The Connallys, The Democrats, The First Lady, The Governor's Mansion, The Great Deception, The Kennedy's, The Kennedys, The Pink Suit, The PRESS, Time LIfe, U.S. Secret Service, United States Secret Service, US Navy, US Politics, US Secret Service, Vince Palamara Tagged 1963, 1997 c-span, ABC, ABC NEWS, Adelle Lot, ADELLE LOTT, assassination of the President, AUSTIN, BARBARA WALTERS, Best Witness: JFK's Limousine, C-span, Cabot, cavalcade, CBS, CBS NEWS, Chauffeur Greer, DAN RATHER, Dealey Plaza, Dealey Plaza Park, Dealey Plaza's National Historic Landmark, Dewitt C. Greer, Dewitt C. Greer State Highway Building, Dewitt Carlock Greer, Dick Greer, Ernie Manouse, EVAN SMITH, EVAN SMITH ON VIMEO, Former Texas First Lady Nellie Connally, Fort Worth, From Love Field: Our Final Hours with President John F. Kennedy, Gary Mack, Governor Connally, Governor John Connally, Greer, he View, Hi quality footage of JFK Assassination, highway commssioner, highway engineer, I have his brains in my hand, I love you, InnerVIEWS, Interstate Highway System in Texas, Ireland, Jack, Jackie, Jackie and Nellie, Jackie Kennedy, Jackie Kennedy's Pink Suit, Jackie Onassis, Jackie triggers a killing mechanism?, JAKE PICKLE, JFK, JFK ASSASSINATION, JFK Assassination John & Nellie Connally On Larry King, JOHN, JOHN B. CONNALLY, John C. Connally, John Connally, John Connally and Nellie Connally, John F. Kennedy, Joy Behar, Jr., Jump Seat, JUNIOR RIED, Kennedy, Kennedy assassination, Kennedy family, Life Magazine, MANOUSE, Meridith Vieira, motorcade, Mr. RANKIN, Mrs. CONNALLY, Mrs. Kennedy, Museum of The Texas School Book Depository, Nancy, Nancy De Graffenried, Nancy DeGraffenried Sayers Abington, Nancy Sayers, Nancy Sayers Abington, NELLIE CONNALLY, Nellie Connally on the View in 2003. Part 1 of 2 parts., NELLIE CONNALLY'S STATEMENT TO THE PRESS ON NOVEMBER 24, North Carolina, Nov. 10, November 22, pink suit, President Kennedy and the Secret Service, Presidential limousine, propellent, Richard Greer, SAD PINK SUIT, SAYERS, SCOTT PHILEN, Scott Philen Sayers, SCOTT SAYERS, Secret Service Agent Greer, Sherri Shepard, State Highway Building, state office building, Texas Department of Transportation, Texas Employment Commission, Texas First Lady, Texas Highway Commission, Texas Highway Department, Texas Historical Commission, Texas House of Representatives, Texas Monthly Talks, Texas School Book Depository, Texas State Cemetery Committee, Texas state highway engineer, The Dewitt C. Greer State Highway Building, the Mansion, THE VIEW, they have killed my husband, Time Life, Travis County, Travis County jails, TRENT LOTT, U.S. Navy, U.s.: JFK Limo's Last Survivor, United States Secret Service, VIMEO, Vince Palamara, WARREN COMMISSION, Warren Commission Testimony of Mrs John F. Kennedy, Warren Commission Testimony of Mrs. John Connally, Was Jackie directly involved in the JFK assassination?, William Carlock Greer, William Greer, William Robert Greer, World War II, Zapruder, Zapruder film Leave a comment
Jackie Kennedy and Nellie Connally
Nov. 10, 1997 c-span "Former Congressman Jake Pickle introduces this one hour discussion from the Lyndon Baines Johnson Library, Austin Texas" "Well, welcome to the um Nellie Connally homecoming. This is her home town and uh thank goodness she has … Continue reading
Posted in Abomination of Desolation, Assassination of President Kennedy, BILLY GRAHAM, Books about the assassination of President Kennedy, Books about the Kennedys, CIA, Jesuits, New World Order, Pillbox Hat, Popes and Presidents, Popes of Prophecy, Popes of Roman Catholicism, Roman Catholocism, SAMARITAN'S PURSE, Secret Service, SS, Synoptic Gospels, The Assassination, The Beast, The Connallys, The Democrats, The First Lady, The Great Deception, The Illuminati, The Kennedys, The Pink Suit, The Signs, The Universal Religion, The Way, Vice President Lyndon B. Johnson Tagged 1963, Acts, Billy Graham, Book of Acts, C-span, C-span | -span, Cap Field, CAPSTONE, Catholic priest, CONNALLY, DALLAS TEXAS, Donald Ewen Cameron, Dwight D. Eisenhower, Evangelicalism, First Lady Nellie Connally, Former Texas First Lady Nellie Connally, Franklin D. Roosevelt, Gary Mack, Gary Mack - On Second Thoughts - Or Should That Be On Third Thoughts, George Gänswein, Great Deception, Henney Motor Company of Freeport, Hess & Eisenhardt co-founder dies, Hess and Eisenhart, Hess and Eisenhart of Cincinnati, Illinois, Jackie Kennedy, James J. Rowley, JAMES J. ROWLEY TRAINING CENTER, Jean Hill, Jesuit, JESUITS, JFK, JFK ASSASSINATION, JOHN B. CONNALLY, John F. Kennedy assassination, Joseph Ratzinger, Kennedy, LBJ, Lincoln Cosmopolitans, Lyndon B. Johnson, Mel Gibson, NANCY GIBBS AND MICHAEL DUFFY, Nellie, NELLIE CONNALLY, North Texas State University, Parkland Hospital, Part 2: The Presidential Bubbletop Limo, President John F. Kennedy, President John F.Kennedy assassination The evidence., President Kennedy and the Secret Service, PRESIDENT KENNEDY'S ARRIVAL AT LOVE FIELD IN DALLAS ON 11/22/63, President Lyndon B. Johnson, Q Source, Rev. Wilkerson, Roman Catholic Church, Roman Catholicism, Rufus Youngblood, Secret Service, SS, Testimony of Gary Mack, TESTIMONY OF JAMES J. ROWLEY, Texas Monthly Talks, The Assassination of President Kennedy, The Kennedys, The Preacher and the Presidents, The PRESS, Twenty Years in the Secret Service, Uncategorized | Blair House, United States Secret Service, Vice President Lyndon B. Johnson Leave a comment


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Double Xp Weekend
Black Ops 2 is hosting a double XP weapon progression weekend starting today
Black Ops 2 players can earn double xp on weapon progression this weekend. The event starts today at 6pm GMT/10am PDT and runs through Monday until 6pm GMT/10am PDT on Monday. This corresponds with Uprising being available for PC and PS3. Go shoot something.
Double Xp Weekend headlines
Black Ops 2 Double XP Weekend is live on all formats
Activision is hosting a Double XP Weekend for Black Ops 2, to celebrate the impending release of the Uprising DLC Pack. It starts today and runs through Monday, April 15 until 10.00am PST. The Double XP weekend is for PC, PS3, Wii U and Xbox 360. The Uprising DLC launched on April 16 for Xbox […]
Black Ops 2 to host double XP weekend
Call of Duty: Black Ops 2 entices you back to its firm, military grip this weekend with the promise of double XP for multiplayer. The fun kicks off at 10:00AM PST on January 25 and runs through the same time on January 28. PC, PlayStation 3, Wii U and Xbox 360 players will all benefit.
Modern Warfare 3 Xbox Live double XP weekend coming
According to MP1st, Activision is running a double XP event for Call of Duty: Modern Warfare 3 this weekend. The news comes via a system message when you fire up the multiplayer, and seems to be an Xbox Live-only affair – just like the recent content drop. Never mind, everyone else – your time will […]
It's a Double XP weekend for Crysis 2 on all platforms
Since it's a holiday in the US, and an impending Bank Holiday in the UK, Crytek is holding a Double XP weekend for Crysis 2 starting today and it ends on Monday 12.00am PST.
Sony announces Double XP Weekend for Killzone 3 multiplayer
Sony is holding a Double XP Weekend for Killzone 3' players in celebration of the return of online to PSN. It is running right now, and will cease on May 23 at 10:00 am UK time. Of note: during the Double XP Weekend, the Score Screen will display the message "Fast Starter Bonus" instead of […]
Black Ops double XP weekend for PS3
What better way to celebrate the return of the PlayStation Network than by enjoying increased Call of Duty: Black Ops XP over the weekend?
First Double XP Weekend for Modern Warfare 2 dated
Infinity Ward's Robert Bowling has tweeted that the first Double XP Weekend for Modern Warfare 2 will happen April 2-5 on Xbox Live.
Get double XP this weekend playing Gears of War 2
This weekend, Gears of War 2 is handing out double XP with public multiplayer matches on Xbox Live.The event kicks off this Friday, May 15 and runs until May 18.So log in and rank up.More through the link.
Get double XP this weekend with CoD: World at War
Call of Duty: World at War players will get double XP this weekend to celebrate the success of Map Pack 1.The double XP applies to all versions of the game and will run through Monday, ending at 1.00pm EST.More information can be found over at the Call of Duty Headquarters.
CoD4 double XP weekend extended until Tuesday
Infinity Ward's announced that the CoD4 double XP weekend running alongside the release of the Game of the Year edition is still running and will last until tomorrow. Heads Up: In light of the map pack taking down Marketplace for a good chunk of time during Friday's launch of the DLC and hence putting a […]
Double Xp Weekend latest
Black Ops 2 is hosting a double XP weapon progression weekend starting today


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To capture the essence of Forty Licks, the 40 tracks that cover the Rolling Stones career, Winemaker Mark Beaman chose to work with Merlot. This varietal captures the comfort of turning to a solid classic that stands the test of time. Much like the band itself, Mendocino County winemaking stays true to its roots with a consistent and confident character not caught up in fads or trends. The 2009 Merlot captures the attitude of Mendocino Countys style. Aromas of black cherry, with hints of mint are followed by rich flavours of plum, black cherry, brown sugar, cinnamon, and cedar. This dry red wine is a fine match for herb-roasted chicken, grilled beef or smoky chili.
Red Wine from TheDrinkShop


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|International orders may be delayed up to 15 days or more due to carrier labor challenges.
The walnut tree blossoms in the spring; its tightly-grained timber is highly valued for its hard, dense resilience, and the way it polishes to a smooth satin finish.
Its Latin name translates to Jupiter's Acorn, or a nut fit for a god. And that's how you'll feel when you open the secure latches of this classically beautiful Greek walnut backgammon set to tackle your next game.
Prepare to scale Olympian heights on a playing surface that gleams with a rich walnut sheen and is elegantly accented with meticulously carved matte gold and ink-black points. Feel the weight of the dice and stones along with the tradition and history of the finest wood backgammon sets carefully created by generations of the Neroulia family of Greece.
Click the specifications tab (above) for a detailed description of this backgammon set, including all materials and sizes. You may click any photo above for a closer view.
|Backgammon Set Dimensions:
|Closed Length:
|18 3/4"
|10 1/4"
|2 5/8"
|Open Length:
|18 3/4"
|20 1/2"
|1 1/4"
|Backgammon Set Materials:
|Case Material:
|Colored Mahogany
|Case Color:
|Field Material:
|Walnut Veneer
|Field Color:
|Points 1 Color:
|Points 2 Color:
|Clasp Style:
|Brass Clasps
|Backgammon Stone Dimensions:
|1 3/8"
|Stones 1 Color:
|Stones 2 Color:
|Backgammon Dice/Cube Dimensions:
|Dice Size:
|Dice Set 1 Color:
|Dice Set 2 Color:
|Cube Color:
|Backgammon Cup Dimensions:
|Cup Height:
|2 1/4"
|Cup Exterior:
|Cup Interior:
|Green Felt
|Cup 1 Outer:
|Cup 1 Inner:
|Cup 2 Outer:
|Cup 2 Inner:
USA & CANADA - shipping via UPS
We offer FREE ground shipping to the contiguous USA and Canada (Hawaii and Alaska are excluded) for many products. Look for the "FREE SHIPPING" designation.
For all other items, and for Express Shipping options, we use an integrated online UPS shipping calculator. The shipping charges are calculated in real time by UPS. 1
Once you have finished shopping, you will be able to select Express Shipping options (Next Day Air, 2nd Day Air, 3-Day Select) on check-out. Only after you know the total charge, will you be asked to enter your payment information. 2
For same day shipping, where indicated, orders must be received before 2pm Eastern Time (New York) in order to ship same day. Orders received after 2pm will be sent out the following business day. Orders received on weekends and on holidays will be processed the next business day.
INTERNATIONAL - shipping via USPS
We offer shipping to over 190 countries with United States Postal Service using Express Mail International. Packages are insured and fully trackable.
International orders can take up to 5 business days to ship. Once your order ships, you will receive a tracking number by email. Customers are responsible for any import charges (tax, duty, etc.)
APO AND MILITARY ADDRESSES - shipping via USPS
Unfortunately, we cannot supply tracking numbers for shipments to APO/FPO addresses. The U.S. Government requires that we send all shipments to APO/FPO addresses using the U.S. Postal Service.
When completing your Shipping Address during checkout, please make note of the following.
* The Country must be "USA"
* The City must be entered as either "APO" or "FPO"
* The State must be selected as either "APO/FPO AA" "APO/FPO AE" or "APO/FPO AP"
* You must enter the zip code for your shipping address
* Always remember to enter your full name, grade, and PSC or unit number.
If you have any questions, please email us at email@example.com, or call us toll free in USA & Canada at 1- 866-260-2058.
1 Shipping charges at all times are based on weight and not on ticket price. There is a minimum 1-pound shipping charge on any item, including small items such as dice or checkers. In these cases, shipping charges can exceed the cost of the item.
2 We offer Express Shipping options on all items except Hillsdale game tables. All Hillsdale game tables ship ground freight.


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That's right, the title means nothing. Move along, there's nothing to see here.
I had EVERY intention of taking photos last night, but hubby and I wound up at the bookstore and I didn't get around to it. We always laugh when I go to the bookstore because it makes me go to the bathroom. Bookstores apparently change my body chemistry and I have to go poo within 15 minutes of arriving. Seriously. Creative supply stores do the same thing to me. Yes, I'm aware you didn't need to know that mental stimuli causes craptacular-itis in me, but I think it's so odd I had to share. Given the frequency of my visits to said stimulating places, I am one lucky unconstipated woman.
Speaking of nastiness, my lovely little nieces are coming over this Saturday to make Halloween yuckies like "boogers on a stick" and "puke stew." It should be fun. We don't have them over often enough.
I'm going to cast on for Klaralund Friday night. Hubby is going to a local football game so I'm footloose and fiber-infested for the entire evening. I also have some felting projects to get going...
Which reminds me, I just signed up for probably the most exciting workshop I'll take in 2005--advanced felt hatmaking with Beth Beede. She is the teacher of the person who taught me and she travels internationally sharing her techniques. I am seriously excited about it. If you are a felter, check out the class. It's being held at the John C. Campbell Folk School in North Carolina. I've been there before and really loved it.
Well, I've flurble-goinked enough for today...as my dad says, "See you in the funny papers."


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Just like ice cream, Ryan Gosling comes in many flavors: There's superhero hunk, talent show MVP, and the most delicious of them all, shirtless. Did we also mention he's good to the very last drop?
We know there's no better way to beat the summer heat than with a ménage à trois that involves your air-conditioning unit and a Netflix queue with Gosling galore, but instead of reaching for the remote, why not break out your old stash of Crayola's and indulge in I LOVE MEL's newest addition to its awesome roster of coloring books? Our friends over at Fashion Etc. revealed that the peeps who brought us the rad Kate Moss Coloring Book are now doling out a Ryan Gosling version — an ode to the dreamy gent and all of his hubba-hubba goodness.
Wish you were his Blue Valentine, or want to relive that Photoshopped abs moment from Crazy, Stupid, Love? Then, snatch up this tome before it's gone for good. Or, take a peek at out fave pages during your Gosling marathon intermission — we won't judge. (Mostly because we've been there, too!)
Photo: Via I LOVE MEL


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Exclusive First: McCain and Brace - the Longest POW in Vietnam History - Describe the Secret Communication at the "Hanoi Hilton" Which
Led to Life-Changing Bond
"[Brace's] story should be told to every American, about what love of country,
about love of one's fellow prisoners and faith in God is all about.
I don't know of a more compelling story that carries those lessons."
- Senator John McCain
Locked Up Abroad Premieres Wednesday, April 17, 2013, at 9 PM ET/PT on National Geographic Channel
(WASHINGTON, D.C. - March 20, 2013) As the longest POW in Vietnam history, Ernie Brace likely endured more than any other POW during the war. Held for almost eight years - from May 1965 to March 1973 - he faced capture, starvation, sickness, torture, solitude, desperation and more. Call him resilient and impressive. A survivor and true American hero. So says Sen. John McCain, who became lifelong friends with him during his own five-year internment in Vietnam.
Brace's harrowing firsthand account of captivity kicks off the ninth season of National Geographic Channel's critically acclaimed international hit series Locked Up Abroad on Wednesday, April 17, at 9 p.m. ET/PT (go to natgeotv.com/lockedupabroad and Twitter at https://twitter.com/NGC_PR). Details of his time as a POW include being marched more than 100 miles through harsh jungles, chained in a bamboo cage 24/7, attempting several futile escapes with subsequent beatings and being buried alive up to his neck.
After three-and-a-half years, Brace was transported from the Southeast Asian jungles to the infamous prison in Vietnam dubbed the "Hanoi Hilton," and finally heard the voice of another American in the next cell: Lt. Cmdr. John McCain, a Navy bomber pilot shot down over Hanoi.
McCain had spent nearly a year in solitary confinement and was desperate for human contact. Though they couldn't see each other, McCain and Brace began tapping messages secretly through the wall. If they were caught communicating, it was an instant beating, but they took the risk and soon shared everything imaginable. However, it wasn't until May 1973, just months after they were released and attending a White House reception held by President Nixon, that they would - at long last - meet face-to-face. McCain recounts, "A guy came up to me and I looked at him and he said, 'I'm Ernie Brace.' I went 'Wow.' It was such an emotional moment for me."
In this first exclusive in-depth interview, McCain and Brace describe their incredible stories, which led to a lifelong friendship. "[Ernie's] is an amazing survival story," says McCain, "an amazing story of heroism ... it should be told to every American."
Locked Up Abroad is a cult cable favorite known for taking viewers inside accounts of capture, incarceration and terror far away from home with intimate personal interviews and dramatic reenactments. This season, we'll hear the firsthand accounts not only of those who were locked up but also of those who were directly part of the story, such as the undercover agent compiling the evidence against the suspect or the person making the drug dealing offers.
Upcoming episodes include stories of Californian surfers arrested in Mexico after the shipwreck of their marijuana-filled yacht, who attempt to dig their way out of prison; a reptile smuggler who travels to Belize to avoid U.S. prosecution but is deported back home; and an English ecstasy dealer living the high life until he is caught by American police.
Two Additional Premieres include:
Locked Up Abroad: Snake on a Plane
Wednesday, April 17, at 10 p.m. ET/PT
(To Immediately Follow "Locked Up Abroad: Vietnam POWs: McCain & Brace")
"I elected to take the snake on the plane. Were there some apprehensions, yes! One bite means almost sure death. There's no question I made a lot of bad decisions." - Tom Crutchfield
Tom Crutchfield, an American carpet salesman living in Fort Myers, Fla., called himself the "Mick Jagger of the reptile industry." With worldwide operations, he successfully smuggled crocodiles, snakes, tarantulas and king cobras around the world. But when Special Agent Ernest Mayer with the U.S. Fish and Wildlife Service begins building the case against Tom, the reptile rock 'n' roll time is about to fade. Tom discovers that he is about to be arrested and escapes to Belize. There, he believes he is free, but Belizean authorities arrest and imprison him. His new cellmates are sleeping on the floor, with no electricity, plumbing, toilets or running water in the cells. Yet when Belize officials deport him back to the United States, U.S. federal marshals are waiting for him.
Locked Up Abroad: Raving Arizona
Wednesday, April 24, at 9 p.m. ET/PT
"Dealing ecstasy was there if I wanted it. Hell yeah, let's do it. I'm feeling like a kind of god.
I'm above the law. No one can stop this. But boy, was I wrong. Now I'm dealing with the mafia. Here I was in one of America's deadliest jails. These guys are murdering someone in
front of my eyes. Game over." - Shaun Attwood
Shaun Attwood left his life in England to follow his dream and became a successful stockbroker in America. After moving to Phoenix, Ariz., he soon finds himself using ecstasy and decides to begin dealing the drug. He spends years living the "high life" as an ecstasy kingpin, with glamorous parties, women, expensive cars and a luxurious house. But when a local mafia boss takes offense, the door opens on a dark criminal underworld. Shaun retreats and goes back to being a stockbroker, but it proves to be too little too late when the police knock at the door. He's thrown in one of America's deadliest jails, and just when he thinks it can't get any worse he witnesses skinheads murdering someone in front of him. His act of defiance: starting "Jon's Jail Journal," a blog describing the unconstitutional conditions inside Maricopa County jails. Sneaking out the writing through visitations with his aunt, his site goes on to attract more than 850,000 visitors.
National Geographic Channel
Based at the National Geographic Society headquarters in Washington, D.C., the National Geographic Channels US are a joint venture between National Geographic and Fox Cable Networks. The Channels contribute to the National Geographic Society's commitment to exploration, conservation and education with smart, innovative programming and profits that directly support its mission. Launched in January 2001, National Geographic Channel (NGC) celebrated its fifth anniversary with the debut of NGC HD. In 2010, the wildlife and natural history cable channel Nat Geo WILD was launched, and in 2011, the Spanish-language network Nat Geo Mundo was unveiled. The Channels have carriage with all of the nation's major cable, telco and satellite television providers, with NGC currently available in 84 million U.S. homes. Globally, National Geographic Channel is available in 440 million homes in 171 countries and 38 languages. For more information, visit www.natgeotv.com.


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Hi there![edit source]
Welcome to our Fallout wiki, and thank you for your contributions! There's a lot to do around here, so I hope you'll stay with us and make many more improvements.
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Water shortages from a persistent drought in the Southwest have left Lake Powell dangerously low, threatening operation of the Glen Canyon Dam hydroelectric power plant relied on by 5.8 million customers.
The problem is a looming concern for reservoirs in the Colorado River basin upstream from the Lake Powell. Those reservoir managers face the possibility of having to deliver water downstream to boost levels and avert a shutdown of the plant.
Reservoirs, including Flaming Gorge, McPhee, Animas-La Plata, Navajo, and Blue Mesa could potentially be tapped for additional water under the "call" system if conditions don't improve in the next one to two years, water officials report.
Now is the time to have the discussion of how to deal with the situation unfolding at Lake Powell, said Mike Preston, general manager for the Dolores Water Conservancy District, which operates McPhee Reservoir at Dolores.
"If Powell becomes too low to operate it would trigger a crisis, so we need to decide early on how we would deal with that," Preston said during a meeting on reservoir operations in Dolores last week.
According to a February memorandum from the Colorado Water Conservation Board, both Lake Powell (of the Upper Colorado Basin) and Lake Mead (of the Lower Colorado Basin) could soon become too low to operate their hydropower plants if conditions don't improve. As of May 12, Lake Powell was at 42 percent of full, and has only been totally filled three times in the last 14 years.
A water-level graph for Lake Powell, created by the Bureau of Reclamation, shows what would happen if 2014-2020 is a repeat of drought-impaired hydrology from 2001-2007.
According to the simulation, as early as 2015 Lake Powell could drop to, or below, the minimum power-pool level required to operate the hydroelectric generators. If the pattern materializes, the level would stay below the power pool for years and by 2020 still not have recovered to power-producing levels.
Allowing Lake Powell to fall below the minimum power pool has numerous dire consequences, according to the CWCB memo:
It would result in dramatically higher electric costs for cities, towns and farms throughout much of Colorado, increasing rates two to four times. The Dolores Project relies on power generated from Glen Canyon sold at a discounted rate.
Funding for irrigation projects derived from power plant revenues would dry up.
Reduced capacity to make releases from Glen Canyon Dam threatens compliance with Colorado River Compact obligations. The result could be litigation and curtailment of water use within the Upper Basin states, which include Colorado.
"In light of these real and immediate threats, the governor's Colorado River representative directed a group of Colorado water advisers to engage six Colorado River Basin states in confidential brainstorming and system modeling for the purpose of developing an emergency response plan," the memo states.
Solutions to prevent a shutdown of power plants at Lake Mead and Lake Powell may involve delivering more water downstream, the memo states. That could impact storage yields from upstream reservoirs on the Green, Gunnison, San Juan, Animas and Dolores Rivers, among others.
In a presentation to water stakeholders Wednesday in Cortez, John McClow, the governor's representative on Colorado River matters, said the guidelines for potential shortages are being negotiated among upper basin states.
"States need to negotiate, not litigate," he said, adding the loss of power at Glen Canyon would be "catastrophic event for Colorado electric rates."
The lack of water in Powell could also limit use of bypass tubes that deliver water downstream to Lake Mead, threatening water compact obligations.
"It is a what-if scenario but there is a 20 percent chance this could occur. We need a plan to deal with this potential crisis," McClow said. "The Secretary of Interior is closely monitoring the situation and is so far satisfied affected states are dealing with it."
Implementing demand-management programs to bolster Lake Powell could also involve voluntary lease-fallowing or deficit irrigation.
"The water-management world cannot be in denial about drought, and we have to be mindful and adaptable," Preston said. "There is already talk about making contributions to bring Powell up. It could be sooner rather than later where we are forced to confront demands larger than our basin."
DWCD is planning to conduct an optimization study of local water supplies as a result of the problem at Lake Powell.
"We want to have a contingency plan on the shelf," McClow told a room of farmers and water managers."The good news is that the Green River was at 170 percent of average this winter and the Gunnison was at 130 percent."


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Ashley Tisdale's Blondie Girl Productions is producing "Cloud Nine".
Creative Commons
Blondie Girl Productions in association with Salty Pictures and Hollywood Farms will begin shooting in January, 2013 on a new Disney Channel Original Movie titled "Cloud Nine". The project is a snowboarding themed telefilm. The local Utah Disney casting directors have scheduled an open casting call for featured and general background performers in Salt Lake City. Snowboarding experience is helpful but not required. All types are needed, and there is no previous experience required to be cast. Filming will continue throughout February, 2013.
Details Here:


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@DJAMEEBLOG UPDATE @Y_MILLZ_GODHOS x @LOVING__JAMES #HITMAN PRODUCED BY LEE BOY. (SUBSCRIBE) THIS IS AN ENTREE OF WHAT'S TO COME IN 2015 FROM DJAMEE MODELS TALENT ARTISTS MANAGEMENT - BE PREPARED TO BE DAZLED BY GREATNESS - DR
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Takei, N., Kawamura, M., Ishizuka, Y., Kakiya, N., Inamura, N., Namba, H. and Nawa, H.
Notes: The authors studied how the basal rate of protein synthesis in primary cortical neurons was affected by chronic treatment of with a variety of neurotrophic factors and cytokines. Rat eukaryotic elongation factor 2 (eEF2) was cloned by PCR then subcloned into the pCI Mammalian Expression Vector and electroporated into neurons. After 72 hours, the neurons were harvested and used in various translation assays including ribosomal transit time. (4070)
Expand Full Notes »
pCI Mammalian Expression Vector
Yamada, K., Takahashi, M., Hoshino, Y., Takahashi, H., Ichiyama, K., Tanaka, T. and Okamoto, H.
Notes: The authors created a full-length infectious cDNA clone of a genotype 3 hepatitis E virus (strain JE03-1760F) for use in cell culture. The full-length ORF2 sequence of the JE03-1760F genome was amplified and cloned into the pCI Mammalian Expression Vector. The construct was transfected into PLC/PRF/5 cells for 3 days then analyzed by Western blotting. (4029)
Wilson, P.M., Fazzone, W., LaBonte, M.J., Lenz, H.J. and Ladner, R.D.
Notes: The authors examined the role of p53 in modulating dUTPase promoter activity. Base substitution mutations of Sp1- and E2F-binding sites in the dUTPase promoter were performed using the GeneEditor™ in vitro Site-Directed Mutagenesis System. Each mutant was confirmed by DNA sequencing. To determine growth inhibition, HCT116 human colon cancer cells were seeded in 96-well plates at 3 × 103 cells/well and treated with 5-fluorouracil (5-FU), fluorodeoxyuridine (FUdR), oxaliplatin or in combination. After 72 hours, the CellTiter® 96 AQueous One Solution was dispensed into each well and absorbance measured. RNA was isolated from HCT116 p53+/+ and HCT116 p53-/- cells. cDNA was reverse transcribed from 200ng total RNA followed by multiplex qPCR using the Plexor™ qPCR System to amplify dUTPase, thymidylate synthase and GAPDH, a housekeeping gene. The 1.2 kb region of the dUTPase promoter upstream of the transcriptional start site was amplified by PCR and the fragment cloned into the pGL3-Basic Vector. Truncated promoters were also generated by PCR and cloned into the same vector. Drosophila SL-2 cells and HCT116 cell lines were seeded in a 24-well plate and transfected with dUTPase pGL3 promoter constructs or with pCI-Neo:p53WT, pCI-Neo:p53MUT and the empty pCI-neo Mammalian Expression Vector; all transfections included the pRL-TK Vector at a ratio of 1:10. After six hours, the cells were incubated in either fresh medium or medium containing a cytotoxic agent at the appropriate concentration. Thirty hours later, the cells were lysed, quantitated by Western blotting and 20µl of lysate analyzed with the Dual-Luciferase® Reporter Assay System. Electrophoretic mobility shift analyses (EMSA) were performed using –64 to –91 of the dUTPase-nuclear isoform transcriptional start site in the Gel Shift Assay System. (4031)
CellTiter 96® AQueous One Solution Cell Proliferation Assay
Dual-Luciferase® Reporter 1000 Assay System
Dual-Luciferase® Reporter Assay System
Dual-Luciferase® Reporter Assay System 10-Pack
pRL-TK Vector
Expand Products »
Ha, V.L., Bharti, S., Inoue, H., Vass, W.C., Campa, F., Nie, Z., de Gramont, A., Ward, Y. and Randazzo, P.A.
Notes: The authors wanted to understand the role of ASAP3, an Arf GTPase-activating protein in cancer cells. FLAG- or hemagglutinin-tagged ASAP3 and GAP-deficient mutants of ASAP3 were cloned in the pCI Mammalian Expression Vector. The constructs were transfected into cells and used in immunofluorescence and Western blotting experiments. (3987)
Fields, M.A., Zheng, M., Wall, P., Oberg, S. and Atherton, S.S.
Notes: Since tumor necrosis factor alpha (TNF-α) is known to have a protective effect on herpes simplex virus type 1 (HSV-1) infection in mouse eyes and brains, a recombinant virus constitutively expressing TNF-α was generated to see if it affected virus location and spread in the eyes and brain. Mouse TNF-α DNA was subcloned from one vector using EcoRI and placed into the pCI Mammalian Expression Vector downstream of the CMV immediate/early enhancer/promoter region. Then the CMV enhancer/promoter::TNF-α region was digested with BamHI and BglII to be placed in a third vector between the UL49 and UL50 genes of HSV-1. This construct and two controls were used to create recombinant HSV-1 particles that were then injected into the eyes of BALB/c mice. (3984)
Gubser, C., Goodbody, R., Ecker, A., Brady, G., O'Neill, L.A., Jacobs, N. and Smith, G.L.
Notes: The authors wanted to examine how the Camelpox virus (CMLV) gene 176R (v-slfn) might affect orthopoxvirus virulence since it shares sequence similarity to murine schlafen (m-slfn) proteins that inhibit T cell and fibroblast growth. HeLa cells were transiently transfected with either pCI Mammalian Expression Vector alone or pCI Mammalian Expression Vector expressing v-slfn with or without a HA tag. Cellular localization of the protein was visualized using either α-v-slfn antiserum or α-HA mAb. (3757)
Schäfer, P., Cymerman, I.A., Bujnicki, J.M. and Meiss, G.
Notes: The authors used biochemical and mutational analysis to characterize human lysosomal DNaseIIα. Native DNaseIIα and site-directed mutants were expressed as Flag-His6-DNaseIIα and HA-tagged DNaseIIα using expression constructs created with the pCI Vector. Protein was expressed by transiently transfecting HEK 293-T cells using the TransFast™ Transfection Reagent. Flag-His6-DNaseIIα was purified using the MagneHis™ Ni-Particles, and this purified protein was used in nuclease assays to monitor catalytic activity and in gel filtration experiments and coimmunoprecipitation assays with HA-DNaseIIα to determine whether the active enzyme is monomeric. (3786)
MagneHis™ Ni-Particles
TransFast™ Transfection Reagent
Kaimori, J.Y., Nagasawa, Y., Menezes, L.F., Garcia-Gonzalez, M.A., Deng, J., Imai, E., Onuchic, L.F., Guay-Woodford, L.M. and Germino, G.G.
Notes: To understand the post-translational processing of PKDH1 protein, which has a role in human disease, a single full-length cDNA of human PKHD1 was assembled from three overlapping amplimers generated from a human kidney cDNA library. This PKHD1 cDNA was cloned into the pCI Mammalian Expression Vector, then N-terminal FLAG or EGFP tags and a C-terminal 7XMyc tag were added for detection. However, the expression level was too low, and an expression-enhancing beta-globin intronic sequence was added between the construct promoter and PKHD1 cDNA sequence. The pCI-PKHD1 construct was transfected into HEK 293 cells and the protein expression assessed using affinity tag capture and Western blotting. (3758)
Zhuang, H. and Matsunami, H.
Notes: To explore the role that accessory proteins may play in successfully expressing odorant receptors (ORs) on the cell surface of heterologous cells, the authors explored cotransfecting receptor-transporting proteins RTP1S, Ric8b and Gαolf with an N-terminally tagged OR to generate a functional and ligand-specific expression system. Three tags (Rho, FLAG or HA) were inserted into the NheI and EcoRI sites of the pCI Mammalian Expression Vector. Amplified OR orfs were cloned into the MluI and NotI sites of the tagged pCI Mammalian Expression Vector. The accessory proteins were subcloned into HA-pCI (RTP1S) or pCI (RTP1S, Ric8b, Hsc70t and Gαolf). For cotransfection, the N-terminal tagged OR vectors (0.8µg) and the accessory proteins (individually or in combination; 0.8µg) were transfected into HEK293T or Hana3A cells. The transfected cells were then subjected to immunocytochemistry, live-cell surface staining, permeabilized staining or FACS analysis. The Dual-Glo™ Luciferase Assay System was used to assess OR activation via CRE elements on a firefly luciferase vector. pRL-SV40 Vector was the internal control for cell viability and transfection efficiency. HEK293T or Hana3A cells were plated on 96-well plates, transfected with 1µg of CRE-Luc vector, 1µg of pRL-SV40 Vector, 5µg of OR and 1µg total for all accessory proteins (0.25µg each protein with pCI Mammalian Expression Vector to keep amount of plasmid constant). Twenty-four hours posttransfection, the medium was changed to CD293 chemically defined medium, incubated for 30 minutes at 37°C then replaced with 25µl of odorant solution in CD293 for a second incubation of 4 hours at 4°C. Then the reporter protein expression levels were measured by luminescence. (3687)
Dual-Glo® Luciferase Assay System
pRL-SV40 Vector
Teixeira, F.M., Teixeira, H.C., Ferreira, A.P., Rodrigues, M.F., Azevedo, V., Macedo, G.C. and Oliveira, S.C.
Notes: To test the feasibility of the Mycobacterium bovis Ag85B gene as a DNA vaccine, the gene was amplified by PCR, introducing EcoRI and XbaI restriction endonucleases onto the primers, digested with the restriction enzymes and ligated into the pCI Mammalian Expression Vector. After transformation, the constructs were isolated using the Wizard® Plus Minipreps DNA Purification System and analyzed using endonuclease digestion and DNA sequencing. To confirm production of a maltose binding protein (MBP)-Ag85B fusion protein in E. coli, the cell lysate was analyzed by Western blot, stained with a rabbit anti-MBP serum and secondary antibody Anti-Rabbit IgG (Fc), AP Conjugate (1:10,000 dilution) and developed with BCIP/NBT. BALB/c mice pretreated with 10mM cardiotoxin prior to intramuscular DNA immunization using construct pCI-Ag85B, administered at days 0, 15, 30, and 45. The immunization course was 50µl of DNA at a concentration of 1µg/µl in PBS with each animal receiving a total of 100µg of plasmid DNA. Antibody response was assessed at 15 days with an ELISA assay and cytokine presence tested two weeks after immunization using splenocytes for ELISA assays and ICC. (3552)
Wizard® Plus Minipreps DNA Purification System
Anti-Rabbit IgG (Fc), AP Conjugate
Gat, O., Grosfeld, H., Ariel, N., Inbar, I., Zaide, G., Broder, Y., Zvi, A., Chitlaru, T., Altboum, Z., Stein, D., Cohen, S. and Shafferman, A.
Notes: After bioinformatics analysis of the Bacillus anthracis genome for potential vaccine targets, the 197 ORFs were selected for amplification. Using the genomic DNA of B. anthracis strain Vollum, all PCR products were generated using a 5' primer incorporating the T7 promoter, the Kozak sequence, unique restriction enzyme sites and a start codon and a 3' primer with a stop codon and three more unique restriction sites. The amplicons were then in vitro transcribed and translated using the TNT® T7 Quick for PCR DNA system and radiolabeled with [35S]methionine. The products were analyzed by SDS-PAGE and autoradiography. To assess the immunoreactivity of the translated products, the proteins were immunoprecipitated using anti-B. anthracis antisera. The seropositive candidate amplified ORFs were cloned into the pCI Mammalian Expression Vector using compatible RE sites. These vectors were introduced into ICR mice using a Helios gene gun system (0.5µg plasmid DNA on 1µm gold particles) and the mice immunized in 2 week intervals. (3515)
TnT® T7 Quick for PCR DNA
Schirmbeck, R., Riedl, P., Fissolo, N., Lemonnier, F.A., Bertoletti, A. and Reimann, J.
Notes: To analyze whether CD8+ T cell responses can be generated from gene products translated from another reading frame from the primary ORF encoded by DNA vaccines, various constructs were made using the pCI Mammalian Expression Vector. These inserts included the small hepatitis B surface antigen (HBsAg) and its 3' untranslated sequence up to the hepatitis B virus (HBV) poly(A) sequence, which contains the sequence encoding the C-terminal Pol344–832 fragment; the 832 residue Pol protein, and the large HBsAg in an alternative reading frame and an unrelated in frame and out of frame OVA OVA18–385 fragment. In the case of the OVA fragment, the CMV promoter was removed and substituted with the SV40 promoter from pSI Mammalian Expression Vector. The immune response to both the primary and secondary gene products was determined using transient transfection of LMH cells followed by Western blotting or immunoprecipitation or counting CD8+ T cells from spleen cells. (3553)
Smith, C.C., Yu, Y.X., Kulka, M., and Aurelian, L.
Notes: The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from 5 x 107 human G361 melanoma cells. The isolated DNA was used for Southern blotting. Probes were constructed with the DNA 5'-End Labeling System. The cDNA for the H11 protein was expressed in 293 HEK cells using the pCI Mammalian Expression Vector. (0075)
DNA 5´ End-Labeling System
Wizard® Genomic DNA Purification Kit
Fu, C.A., Shen, M., Huang, B.C.B., Lasaga, J., Payan, D.G., Luo, Y.
Notes: The pCI Mammalian Expression Vector was used for transient expression of the 150kDa TNIK protein in Phoenix-A cells, a 293 derivative. (1126)
Demolombe, S., Baro, I., Pereon, Y., Bliek, J., Mohammad-Panah, R., Pollard, H., Morid, S., Mannens, M., Wilde, A., Barhanin, J., Charpentier, F., Escande, D.
Notes: The pCI Mammalian Expression Vector was used to express human cardiac KvLQT1 isoform 1 and KvLQT1 isoform 2 in COS-7 cells. Transfected cells were used for electrophysiology studies. (1270)
Apergis, G.A., Crawford, N., Ghosh, D., Steppan, C.M., Vorachek, W.R., Wen, P. and Locker, J.
Notes: This paper describes use of the PolyATtract® mRNA Isolation System, pCI Mammalian Expression Vector, and TNT® Coupled Wheat Germ Extract System for RNA isolation, gene expression and gel-shifts assays (EMSA) respectively. (1477)
PolyATtract® mRNA Isolation System I (Refill for Z5200)
PolyATtract® mRNA Isolation System II with Magnetic Stand
PolyATtract® mRNA Isolation System III with Magnetic Stand
PolyATtract® mRNA Isolation System IV (Refill for Z5300)
TnT® SP6 Coupled Wheat Germ Extract System
TnT® T7 Coupled Wheat Germ Extract System
TnT® T7/SP6 Coupled Wheat Germ Extract System
Memon, S.A., Hou, J., Moreno, M.B., Zacharchuk, C.M.
Notes: The pCI Mammalian Expression Vector was used to express a dominant negative version of FADD containing only the death domain. (0678)
De Angelis, D.A., Miesenböck, Zemelman, B.V., Rothman, J.E.
Notes: In this paper, various constructs of GFP-polypeptides were prepared in the pCI Mammalian Expression Vector. The constructs were expressed in HeLa cells. (1319)
Diener, K., Wang, X. S., Chen, C., Meyer, C.F., Keesler, G., Zukowski, M. , Tan, T.H., Yao, Z.
Notes: Full-length 885 amino acid Germinal Center kinase-like kinase and a C-terminal deletion mutant were expressed from the pCI Mammalian Expression Vector in 293 cells. (1233)
Huber, M., Yee, V.C., Burri, N., Vikerfors, E., Lavrijsen, A.P.M., Paller, A.S., Hohl, D.
Notes: The pCI Mammalian Expression Vector was used to express mutants of the 97kDa protein in cultured keratinocytes. The vector was also used to construct a transfection control vector, pCI-β-Gal (1030)
Smith, C. L., Htun, H., Wolford, R. G , Hager, G. L.
Notes: Several proteins were transiently expressed in the 1471.1 cell line using the pCI Mammalian Expression Vector. (0379)
pCI-neo Mammalian Expression Vector
Brimecombe, J.C., Boeckman, F.A. and Aizenman, E.
Notes: Green fluorescent protein was subcloned into the pCI Mammalian Expression Vector. This construct was then used a positive control for transfection of CHO-K1 cells. (1407)
Adkins, H.B., Brojatsch, J., Naughton, J., Rolls, M.M., Pesola, J.M. and Young, J.A.T.
Notes: An immunoadhesin-IgG Fc fusion protein was expressed into 293 cell supernatants using the pCI Mammalian Expression Vector. The expressed protein was used for in vitro binding studies and the Fc fusion was used as a means of isolating the complex with protein A. (2060)
Zhang, Y. , Allison, J. P.
Notes: The pCI Mammalian Expression Vector was used to construct a hemaglutinin-tagged transient expression vector. The vector was used to express AP50 protein in 293T cells The expressed protein was reacted with a GST-fusion of CTLA-4 immobilized on glutathione beads and analyzed by Western blots with an anti-HA antibody. (0100)
Patel, A. J., Lazdunski, M., Honore, E.
Notes: The cDNAs for Kv2.1 and Kv9.3 were subcloned into the pCI Mammalian Expression Vector and microinjected into Xenopus oocytes. The proteins were expressed either separately or together and tested for interaction by immunoprecipitation. The proteins were also transiently expressed in COS-M6 cells and used for electrophysiology studies. (0552)


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Wreckage and a messy Bloomberg media hit in Rockaway Beach
It was my first time in the Rockaways in years when I went this weekend. The sun was rising and the light was beautiful.
A segment of boardwalk, weirdly intact, was pressed up against a house on Shore Front Parkway.
Another section was siting in the parking lot 200 feet further inland, complete with a guard rail and several benches facing the water, which was gently lapping the concrete pylons that now appeared to be holding up empty pieces of sky.
Street signs for Beach 94th Street and Beach 95th Street were strewn around. On the parkway, a pole leaned like the Tower of Pisa, with an American flag still waving from it. Up the parkway, a concrete staircase stood, connecting to nothing.
I'd gotten to Rockaway Beach at 7 a.m. on Saturday. Some people in heavy jackets were standing by Cross Bay Parkway, near a Verizon charging station. Some were smoking cigarettes. Nobody looked like they had had a shower.
One block south down Rockaway Beach Boulevard, Surfside Bagel, normally busy on a Saturday morning, was a wreck. The sidewalk and street were covered in sand. A guy in front of the store was smoking a cigarette. Behind him, through the window, I saw a pile of small potato-chip bags in the middle of the floor. Everything else was dark.
I walked back to the crowd at Cross Bay Parkway, where a New York 1 reporter from the rotating crew that has been set up since the storm near political director Bob Hardt's house was conducting an interview. Off to the side, a young man with a stubbly beard and a girl with pink and diamond earrings and a tattoo behind her ear were talking. He said something to make her laugh.
More people were joining the crowd, each one with a sad story, the same story. I left and walked north.
There was a mobile library parked on the sidewalk. Across the street was a fire house, doors open, no one visible inside.
Earlier that morning I'd spoken with a photographer in a jacket that had the words "News" and "Fire" in big letters on his back, who told me he was out Monday night photographing the storm. He said he was out "with the gangs." They didn't bother him, he said, but they were ripping stuff off of boats and people's houses. He said he took their pictures and that stopped them from stealing anything else while he was around, but he said they were everywhere. He asked me what time Kirsten Gillibrand and Janet Napolitano were going to be in the area. I told him I wasn't sure and he walked away.
I saw a group of people in dark clothes walking along a sidewalk near where the boardwalk was. As I approached them I recognized a few members of Mayor Michael Bloomberg's security team. The only event on his public schedule was a 4 p.m. press conference in City Hall. He had announced, in a press release the day before, the cancellation of the New York City Marathon, after enduring pressure from the public, his own aides and the organizers of the marathon.
Bloomberg was there, in a brown leather jacket, walking-and-talking to one of the anchors from Channel 2, Maurice Dubois. There was no other press there.
I tried to get close without alarming his security team or the uniformed police officers. I didn't have my press pass and I was wearing dark blue jeans, white sneakers and five layers of shirts and sweaters--clothes suitable for post-flood clean-up work, which I'd gone out there to help at a friend's house.
I took a picture of the mayor with my iPhone and tweeted that he was in the Rockaways.
Then I hurried back to the New York 1 reporter and crew and told them the mayor was about a block away. We went back and Bloomberg was now in front of the fire house, within eyeshot of the crowd by the Verizon charging station, which was getting a little bit larger.
Grace Rauh of NY1 got her microphone in front of Bloomberg as he was talking to a young African-American girl who seemed awestruck to be talking to the mayor. She was smiling and looked slightly dazed.
Bloomberg jokingly said he was "sorry" but she'd have to be heading back to school shortly. She kept smiling.
He put his arm around her and turned to face his photographer, who took a picture with a small, point-and-shoot-camera (the girl's?) and then with the big digital camera hanging from around her neck.
Then the event went off-plan. A man in a sweatshirt who had come over from the charging station yelled out to the mayor, asking about water. Bloomberg said something about it arriving at 1 p.m.
This made the guy angrier. He didn't want to hear that he'd have to wait till the the afternoon, after waiting all night for what he needed.
Then a woman began yelling. And then another man. NY1's camera was still rolling, and I was trying to get my video camera on the people yelling, and on Bloomberg before he disappeared.
The mayor stood there and pulled in an aide to answer some of their questions.
Rauh asked Bloomberg if this was the kind of response he expected. Bloomberg tried explaining that in an emergency, the response never comes fast enough and this outrage is what you'd expect.
He walked a little bit further, moving the growing cluster of security guards, uniformed police officers, press aides, reporters and onlookers with him. Finally, he got into a black S.U.V. and drove away.
Rauh went into the street and began recapping the whole event into the video camera. A few people passed by behind her. I stood watching her on the sidewalk as a woman in sweatpants walked her dog. I asked her, somewhat stupidly, how she was doing.
"I'm fine," said the woman. Clearly she wasn't, but she wasn't going to complain. She said she didn't have a television and just wanted to know what was going on. She found it a little funny that she was getting to watch the television news happening live.
Talking about her dog, a small white fluffy thing, she said, "He was going crazy in the house," where he had been stuck for a few days. Now, outside, the whole area smelled different, and with the sand everywhere, it felt different too.
"He doesn't know where he is," she told me.


text:
Fairhurst - 1st floor - LFA/144 - Morning
Event box
Print the page Add to a Calendar using iCal Share page on Facebook Add to Google Calendar Share page on Twitter
Fairhurst - 1st floor - LFA/144 - Morning
The teaching space on the first floor of Fairhurst. There are 12 desks in this space.
Directions: Along the Fairhurst corrdor to the far stairs. Up to the first floor. LFA/144 is opposite, to the right.
Related LibGuide: Coronavirus and the Library
- Date:
- Tuesday, January 19, 2021 Show more dates
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No one denies that he broke into Stratfor's computers (as well as other sites and even governments). However, many people quite reasonably argue that he was doing so for the purposes of activism, not for personal wealth or benefits, and that fact should have been taken into account in his sentencing. The DOJ, of course, want to use Hammond as yet another example case of how they can throw the book at hacktivists. The Sparrow Project has a good account of what happened in the courtroom:
Jeremy's lead counsel, Sarah Kunstler, who is 9 months pregnant and due to give birth today, delivered a passionate testimonial as to the person that Jeremy is, and the need for people like Jeremy during our changing socio-political landscape. She was followed by co-counsel, Susan Keller, who wept as she recalled her experiences reading the hundreds of letters from supporters to the court detailing the Jeremy Hammond's selflessness and enthusiastic volunteerism. She pointed out that it was this same selflessness that motivated Jeremy's actions in this case. She closed her testimony by underscoring that, "The centerpiece of our argument is a young man with high hopes and unbelievably laudable expectations in this world."
They also include Hammond's statement, in which he clearly states why he did what he did, and repeatedly points out that most of the sites he hacked (including Stratfor and foreign governments) were done under the direction of Sabu (real name: Hector Xavier Monsegur) who had already turned into an FBI informant. In other words, he's suggesting that the FBI was more or less telling him who to hack, and then they get to turn around and throw the book at him.
The acts of civil disobedience and direct action that I am being sentenced for today are in line with the principles of community and equality that have guided my life. I hacked into dozens of high profile corporations and government institutions, understanding very clearly that what I was doing was against the law, and that my actions could land me back in federal prison. But I felt that I had an obligation to use my skills to expose and confront injustice–and to bring the truth to light.
Could I have achieved the same goals through legal means? I have tried everything from voting petitions to peaceful protest and have found that those in power do not want the truth to be exposed. When we speak truth to power we are ignored at best and brutally suppressed at worst. We are confronting a power structure that does not respect its own system of checks and balances, never mind the rights of it's own citizens or the international community.
The full statement is long, but well worth reading. The court forced everyone to redact part of the speech — where he names who else he hacked at the direction of Sabu, including foreign governments. When you think about this, it seems particularly obnoxious. Basically, the FBI had Sabu tell Hammond to hack into the computers of foreign governments and now Hammond gets the book thrown at him because of that. Does anyone think that the feds didn't make use of that access to foreign government computers? It's a pretty neat trick: trick a hacktivist to break into the computers of foreign governments for you and then throw him in jail for ten years.
In an interview Hammond gave to The Guardian prior to the sentencing, Hammond notes that his days of hacking "are done" but remains pretty defiant and supportive of hacktivism in general and against oppressive government action. He notes that one of the reasons he was such a target was he had access to an exploit that it appears the NSA didn't yet have, which allowed him to get into those foreign government servers:
"I felt betrayed, obviously. Though I knew these things happen. What surprised me was that Sabu was involved in so much strategic targeting, in actually identifying targets. He gave me the information on targets."
Part of Sabu's interest in him, he now believes, was that Hammond had access to advanced tools including one known as PLESK that allowed him to break into web systems used by large numbers of foreign governments. "The FBI and NSA are clearly able to do their own hacking of other countries. But when a new vulnerability emerges in internet security, sometimes hackers have access to tools that are ahead of them that can be very valuable," he said.
In that same interview, he notes he never would have hacked Stratfor if it weren't for Sabu, noting he'd never even heard of the organization before that.
Clearly, Hammond broke the law. But it seems very, very wrong that the federal government clearly used him to break into places they wanted to get into (all of which now remains classified), and then threw the book at him and will lock him up for a decade.
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Amalfi Women's Watch - Silver / Black / Black
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Product description
- Case diameter is 35 mm
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text:
*Due to the current lockdown, UK deliveries may take a few days. We thank you all for your understanding, and ongoing support during these uncertain times. Subscribe to our mail out, for latest news and access to any loyalty codes.
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MATERIAL: Suede
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FIT: We recommend half a size smaller.


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Turkey Shoots Down Syrian Helicopter
A Turkish warplane shot down a Syrian helicopter that had violated Turkish airspace Monday, underlining the regional combustibility of Syria's conflict despite frenzied international diplomacy. Dr. Joshua Walker, former State Department Advisor, discusses.
This transcript has been automatically generated and may not be 100% accurate.
... the ... the data series A helicopter that it says that while it it's it's it's Monday ... in the latest reminder that the conflict in the Middle East poses risks of escalation ... so that ... joining us now to talk about this and the issue of ... border on problems in the region ... is I just go walk to the director of global programs as the ... rest of the fam at Co will why ... he's also former senior advisor to the study by thanks adjoining stuff ... thanks for having me ... so ... in this is one of the concerns around them in a clearly now ... the rupture in the in Russia and the US on trying to work to get some solution that the steals of the chemical weapons concerns ... in Syria because the civil war is continuing ... I would put two million refugees spilling across all the various borders ... of course this isn't telling us that the concerns best images on the third quarter and the prospect of ... reactions ... from serious nighters ... the situation look a mean how how much of the allocated have died this ... is the Middle East room ... I mean I was just there last weekend at our office there in a heated in Istanbul and of course that that time everyone was talked alot about the allin pics but the real question the underlying everything was what's going on in Syria ... which is surprising innocence of this conflict is big on offer of ... over two years now but it seems to reach the boiling point I think ever since August we first in the chemical weapons ... the entire region is on an age Turkey in particular has been very anxious they had a clean shot down a year ago ... the most recent ... insane you just listen to happen yes they are the helicopter ... it's really showing that Turkey is beginning to flex its muscles ... in away we haven't seen in awhile I think in particular given that the US and Russia have reached this deal ... on some of the neighbors and Indian serious neighborhood protecting Turkey are very unhappy with the way things are going ... and seem to want to find some type of solution on their own in the deal to go beyond just the way we've seen this in the past just the Russians and Americans in the time ... great powers ... so what about ... on the the the Bora palms of course Iran is were the ones that everyone is watching here ... in a if if Turkey would be brought into the conflict ... does this play out broadly into some of the other ... neighboring countries ... especially around me I think it ... I he actually doesn't mean Syria has always been more than just about Syria itself it was just about Syria would you be an isolated incident ... but it borders very important that countries like Turkey a rock Levanon Jordan all of these countries with the exception of Turkey are ... unstable in some different ways and given the fact that Iran has you Syria for so long as a proxy given Hezbollah has entered into Syria and a Spidey on the side of the side ... it's become a real regional conflict what's interesting about this conflict is ... and those that oppose us I have not been as willing to put the skin in the game when I mean by that is Iran or Russia have gone to the max EADS time to defend the side whereas the West the United States Saudi Arabia Qatar Turkey other allies are willing to support the rebels but not fully step in the way that the others happen so as result this is becoming much more than just about Syria were seeing it play out the UN were seeing it play out in detail we're seeing it play out in almost every international place that we've seen around the world right now ... what about the allies of the of the rebels us of course being the most important ... thing that I mean ... it really Obama is looking at it very merits of the solutions right now I'm ... he has all thrives on in them the best it's been taking ... quite some time ... is a chance that that ... that they can step up that effort ... I think one of the biggest challenges is it's hard to say though rebels you have to put in as Sony and there are so mean different factions are rebels no one exactly knows what this opposition looks like we all know that are United against the side ... oh we don't know what they stand for in particular in the northern part of Syria ... we have a Kurdish faction you that is the list action ... you have moderate factions ... no one knows what happens in the day after if there is this is a D after which Assad leaves who comes into power in all the differ regional actors in the United States and European players all have different preferred options at this point in time and so it's all these outside actors are able to come together as one unified rebel group it's very very difficult to see this going in a more positive direction right now it seems like we're seeing the splintering of Syria ... that the civil war will continue ... and that unless were a will that some of the unified force that is able to intervene ... this this this killing could continue ... with its gonna be some set of Plan B Libya plan C is well it's the ... sitting ... in a draw somewhere I'm ... you know what sort of ... idea other than the limit instructs what can ... the allies that ... idea Display or unplanted de EME ... bikini body with expenses get to this point ... I do think that there's some very smart people in the U S government are allied governments around the world were thinking about these options but as you said are ready they're just not me need enough to this point in time that the American public most ally ... of the public's including Turkey ... are not really keen on doing anything in the Turkish government has been out front in terms of its opposition to this most recent deal with the side and says we need it that way to get rid of the site of the Turkish population the American population ... we don't seem to want to do anything at this point I'm a ... lot of people are sick of military interventions people are sick of seeing ... the foreign troops in the Middle East and a basic but the hands of the said look the Syrian people have to decide this ... question what can we do to tip the scales the weather is limited airstrikes whether shooting down helicopters and really eliminating Assad's ability to move around as though ... his own country what is that we didn't do it this point I mean I think we're all struggling to tread a fine dancer think everyone wants to see the killing in business possible ... but that gesture will thank you very much to time


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rock of ages
Tower Rock, Wittenburg
Jarrett Medlin
The great rock is difficult to
find but hard to miss. To reach the landmark by land, you must travel
along miles of narrow roads in rural Perry County before coming to
a gravel stretch that hugs the Mississippi River. Then, as you round
a bend, it suddenly comes into view — Tower Rock, Grand Tower
Roc or La Roche de la Croix.
The limestone island rises 90 feet above the mighty river and instantly
captures the imagination of visitors, as it has for centuries.
American Indians once believed the rock housed a "manitou," a
demon that devoured visitors and passing boats. Explorer Pere Jacques
Marquette later discovered there was no evil spirit, but rather a dangerous
eddy between the rock and the shore that roars during high water. In
1803, explorers Lewis and Clark noted rivermen who passed the rock
would celebrate in a way similar to sailors crossing the equator, by
raising a drink.
Even today, new legends persist
about the island. Some say the rock is the nation's
smallest national park, a rumor started by "Believe It or Not" author
Robert Ripley. Others believe the island is a Lutheran "Plymouth Rock" for
followers of Martin Stephan, though there's no such record. Finally, there
is the question of ownership. Perry County, the state of Missouri and the federal
government have all asserted claims at one time.
History and legend withstanding,
Tower Rock is an impressive sight. To see it for yourself, take Highway 61
to Highway A and head east. Keep going past Wittenburg and look for
a small sign that reads "Tower Rock."


text:
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text:
All driving game aficionados will be pleased to hear that Gameloft is working on the next installment in the Asphalt series and the graphics look stunning! Asphalt 8: Airborne, which is scheduled to be released this summer, includes new maps, a new physics engine, and new weather effects. Dubbed "Airborne" because of the off ramps that launch your car into the air for some spectacular stunts, the new game will only cost $0.99 when released!
The game will feature eight maps in total. Seven of these are completely new and include the Nevada desert, the city streets of London, the water-soaked streets of Tokyo and frozen tracks from Iceland. The eighth map is a revamped version of the Monaco track from Asphalt 7, something that will please fans of the current installment.
Asphalt 8 will also feature some heavy duty multiplayer options including both synchronous and asynchronous modes. Takedown mode is returning and there are also two new multiplayer game modes called Gate Drift and Infected. In Gate Drift mode you challenge your friends in a "drift" race through multiple gates with more more being earned when you get through a gate. The Infected multiplayer mode is a came of chase. One player starts as the "infected" player and must hit another car to pass on the infection before their health fails. Doesn't sound too hard? Well you only have 20 seconds to pass on the infection before you are knocked out of the game!
When using an asynchronous multiplayer mode, something that has been popular in games like Real Racing 3 and GRID 2, you can compete against your friend even when they are offline. In short this time shifted mode allows you to compete against your friends previous best performances which are simulated while you are racing.
Gameloft has released a teaser trailer and it looks very impressive especially when you consider the price tag!


text:
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text:
InvestigaciónMar. 7, 2013
EE.UU: publican informe "2011 Pesticide Data Program" sobre la presencia de residuos de plaguicidas en alimentos frescos y procesados
El estudio demuestra que los residuos en alimentos están muy por debajo de las tolerancias de la autoridad sanitaria de los Estados Unidos.
(UPDATED COVERAGE, Feb. 28) An annual U.S. Department of Agriculture report on pesticides on fruits and vegetables once again shows that residues are below government tolerances.
Tom Karst
The USDA's Agricultural Marketing Service 2011 Pesticide Data Program report also includes a letter explaining the levels of residue pose no health risk.
"Consistent with guidance from health and nutrition experts — and as affirmed federal nutrition guidance that urges people to make half their plate fruits and vegetables — we encourage everyone to continue to eat more fruits and vegetables in every meal and wash them before you do so," according to the letter.
The new data confirms that pesticide residues in food "do not pose a safety concern for Americans," according to the Environmental Protection Agency, in the letter.
The USDA's report has been consistent in its message that pesticide residues shouldn't be a concern for consumers, said Kathy Means, vice president for government relations and public affairs for the Produce Marketing Association, Newark, Del.
"One of the things that is most important is that government is now offering some context around (the data) and we really appreciate that," she said.
Marilyn Dolan, executive director of the Watsonville, Calif.-based Alliance for Food and Farming, said Feb. 28 she has observed little consumer media coverage of the USDA report compared to the heavy media coverage that accompanies the release of the Environmental Working Group's Dirty Dozen report. The latest Dirty Dozen list was released by EWG in June last year.
"We're just really trying to encourage reporters to read that PDP report before they do the story on the Dirty Dozen list," she said. The group continues its campaign to educate consumers about the safety of both conventional and organic produce, with the website www.safefruitsandveggies.com as the centerpiece.
In the PDP summary, the agency said sampling was carried out by the AMS in California, Colorado, Florida, Maryland, Michigan, Minnesota, Montana, New York, North Carolina, Ohio, Texas, Washington and Wisconsin.
Samples are selected without regard to country of origin, variety, or organic labeling, according to the report. Fresh and processed fruit and vegetables accounted for 82.3% of total samples collected in 2011, with other samples including water (6.6% of samples collected), milk (5.8%), eggs (2.9%) and soybeans (2.4%). Fresh and processed fruits and vegetables tested in 2011 were samples baby food (green beans, pears, sweet potatoes), canned beets, cabbage, cantaloupe, cauliflower, cherry tomatoes, hot peppers, lettuce, mushrooms, onions, orange juice, papayas, plums, snap peas, canned and frozen spinach, sweet bell peppers, tangerines and winter squash.
According to the USDA, 72.7% of the samples were from the U.S., 22.8% were imports, 3.8% were of mixed origin and 0.7% were of unknown origin.
Excluding water, residues exceeding government established tolerances were detected in 32 of the 11,894 samples. Of those 32 samples, 78% were imported items.
Ver estudio completo


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THE SQL Server Blog Spot on the Web
The information in this blog post may be of interest to anyone out there that is using Continuous Integration with msbuild in order to build/deploy a project that includes SSIS packages.
The MSBuild Extension Pack at http://msbuildextensionpack.codeplex.com/ now includes an MSBuild task that will build an SSIS project, the output from which is a .deploymentmanifest file that can be used in concert with the SSIS deployment utility.
Building a SSIS project using the SSIS MSBuild Task essentially has the same effect as right-clicking on a SSIS project and selecting Build with CreateDeploymentUtility=True (i.e. produces a .deploymentmanifest file):
The advantage of using the MSBuild task is of course that you can now do it in an MSBuild script, none of this manual mouse-clicking required thank you very much!
The SSIS MSBuild task was previously available in the SSIS community samples project at http://sqlsrvintegrationsrv.codeplex.com but having it as part of the MSBuild Extension Pack is definitely advantageous as this is a very widely used set of utilities. The source code has been available in the MSBuild Extension pack since changeset 54481 which was checked-in on 26th August 2010 and was included in the August 2010 Release.
Note that in order to deploy your packages after using the SSIS MSBuild task you will need to call the SSIS deployment utility and pass in the outputted .deploymentmanifest file. Information on doing that is decidedly thin on the ground so I'll try and elucidate. The SSIS Deployment Utility is an executable that gets installed with SQL Server Integration Services workstation tools and is called dtsinstall.exe. You can call it on the command-line and pass in the path to a .deploymentmanifest file but as far as I can determine there is no way to do an unattended install (i.e. there is no way to tell dtsinstall.exe where to install the packages to, it simply opens up a GUI and prompts you for this information). This is a horribly antiquated method of deployment and I'm hunting around to see if there's anything I've missed here – if you know any better please let me know.
Hope this helps!
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I don't think you can automate deployment to the server using a deployment manifest and dtsinstall, you could maybe use the DTUtil.exe for that by calling it for each package in the deployment folder?
Yeah that's what I figured. Which, it seems to me, kinda defeats the purpose of the MSBuild task.
I've always just bypassed the manifest and used SMO to upload the packages. In which case all the CI build actually needs to do is copy the DTSX packages from the project source tree to the output directory (there's no actual *building* going on anyway).
So whilst the MSBuild task is useful, in that it replaces the 'copy DTSX to output directory' part of the process above, I'll almost certainally stick to PowerShell and SMO for deployment due to the flexibility / unattended install.
I can't seem to find any msbuild task for SSIS in this extension set you refer to, is there something i'm missing? Is it a sql server database build task that will work with ssis perhaps?
Hi William
If you check MSBuild.ExtenstionPack.tasks file in Solutions\Main\Common, you can find the following.
<UsingTask AssemblyFile="$(ExtensionTasksPath)MSBuild.ExtensionPack.dll" TaskName="MSBuild.ExtensionPack.SqlServer.BuildDeploymentManifest"/>
This is the task name that you should use in the MSBuild script. However, integration with the SSIS community samples project is only done for 2008. Therefor this will only create SSISDeploymentManifest file. Integration for 2012 hasn't done yet. For more information please check out the following link.
I am using a PowerShell script (with SMO) to deploy from the ispac file.
The script creates a folder in ICS if needed, deploys to ICS and sets any project parameters that may be needed based on the environment.
I am doing this because I need to deliver SSIS projects to other sites to be installed by a non-developer.
Jamie, Have you done any more work on this? I followed the instructions in Yong's comment above and built the dll for Visual Studio 10, but now I am trying to work out how to take the next step and get this automated in a TFS Build server as you did for SSDT database projects in another post. Any insight in how to take the dll and use it? What in the build definition tells the server what type of build to use? The post Yong mentions uses the command line and a pre-built build proj file.
Hi William,
Apologies for taking so long to reply.
To be honest its so long since I wrote this (and equally so long since I attempted what is described here) that I'm not too sure. I suspect the compiled DLL will have to be a part of your code base - that way you can reference it from an msbuild script and know for sure that it will exist.
"What in the build definition tells the server what type of build to use"
I'm afraid I'm not sure what you mean by "type of build". Can you elaborate?


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