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When you're a thinker, like me, you are also a forgetter. You have so much thinking going on in your brain you can't remember the thoughts of moments before. You are always starting "afresh" with a clear purpose so lying around the house are plenty of journals, notebooks and files with clearly stated goals and how to achieve them, to do lists, to don't lists but then you turn the pages and there's nothing else written. Maybe a little doodling – those unmanifested thoughts – possibly some Day 1 dieting and exercise information all written with good intentions. Being a thinker means you can think about your hated weight all day long while still thinking about the worries of your children, what they'll grow up to be, what the hell is for dinner, whether to go left or right at the intersection, if your friend is really doing okay and what you'd write a novel about if you had time.
Being a thinker like me means you don't live in your heart. And when you do "go there" it's for other people's journeys, their pains, hardly ever for yourself and when you do it's because you have "thought" your way there. Being a thinker like me means you can cry deeply when your friends are in any type of emotional pain, rarely for our own. You talk about it but you don't feel it. Sure you can still cry after an argument. You can hyperventilate to a friend about something that fucked you off. You can sob when you watch a sad movie whether it's a true one or not but still your own story is emotionless. It's "just a story" that you put on repeat.
When you are a thinker you forget to breath because breathing would cause you to relax. Breathing brings an opportunity from the neck down to open up. Opening up scares you. Opening up means you have to feel real stuff and feeling real stuff is scary. What if feeling real stuff means you lose it and never regain consciousness, hell worse still you become completely conscious and everything you've known life to be changes. What if those changes aren't what you thought they would be.
Being a thinker means this post is just thoughts. It's talking about feelings but not feeling those feelings. Being a thinker means feeling those feelings is rare.
One Love


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Cote D'Ivoire 6-13 FEBRUARY 2009
Following some logistical problems with visa application Maciej Smietanski and I arrived to a VIP welcome at the Felix Houphouet-Boigny airport on a humid afternoon with grey tropical rainclouds circling the Ebrie lagoon. To an Anglophone the capital city Abidjan is a big surpise. It has a population of 3.5 million and it is the second largest French speaking city in the world. It is also a cultural hub for a multiethnic population and has a large French community. Although the recent civil war has impacted seriously on economic activity, the business district stands out from all sectors of the city with its gleaming skyscrapers, boutiques and outdoor cafes.
We were provided with a car and chauffeur and hotel accommodation paid for and organised by the protocol of the First Lady. Hotel Ivoire was positioned on the edge of the Ebrie lagoon overlooking several converging peninsulas and islands connected by bridges. Here we enjoyed African style comfort in readiness for the hard work ahead.
The Unite Chirurgicale Yopougon-Attie (UCYA) Hospital was situated approximately 30 minutes drive from the hotel and we travelled there to meet Dr Max Thalmus Director of the Hospital. After a tour of the clinic we attended to preoperative assessment of our first 13 challenging inguino-scrotal hernias. The conditions were basic but softened by the presence of air-conditioning. Cleanliness and surgical protocol were perfect and we worked with a cohesive team of Nurses and anaesthetists who provided many spinal anaesthetists for the patients with larger hernias. Our French was a little rough but our surgical vocabulary increased remarkably during the week. Our schedule included three infant hernias, two incisional hernias and one orchidectomy, for a total of 52 patients with 57 hernias. There were no immediate postoperative complications.
We enjoyed immense hospitality including two late night dinners by the lagoon watching fisherman cast their nets into the murky waters ready for the next days table. Desire Trazo, an old friend from Takoradi and an Ivorian citizen dined us out at a Lebanese restaurant and Jean-Didier Guigrey, the instigator of this mission who is the CEO of EOG Healthcare Limited entertained us at his relatives house in the old Colonial Capital of Bingerville with a traditional repaste. We visited some of the architectural wonders of this exotic city including the dramatic St Paul s Cathedral designed by Aldo Spiritom and the Business district.
The highlight however was a meeting with the First Lady coordinated by Jean-Didier and Dr Eugene Djedje, technical advisor to the First Lady. At this meeting the First Lady confirmed that she would give continuing support to all Operation Hernia missions in Abidjan.
It is hoped that in 2009 two to three further teams will attend. I am asking for French-speaking volunteers.
Andrew Kingsnorth
February 2009

subset: commoncrawl


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The Gwinnett County Jail – the largest of the Sheriff's Department's four divisions –
is a direct supervision pretrial detention facility. The jail was constructed in 1991 as a result of overcrowding in the 1980s and replaces the previous linear jail,
which had been used since 1974. The maximum capacity of the jail in 1991 was 576 inmates, but in 1997 the facility had to double-bunk non-specialty beds. By 2002,
the gymnasium was renovated adding an additional 182 beds – bringing the maximum capacity to 1,274.Near the end of 2006, the Gwinnett County Sheriff's Department
completed and opened the new "Wimberly" Tower. The Tower was named after Deputy Ken Wimberly who was tragically killed in the line of duty. This new construction is part of
the expansion plan for the Gwinnett County Jail. Overcrowding at the original direct supervision facility forced the county to add more bed space to the existing site.
The first was the completion of the "Wimberly" Tower and the second will be an additional tower at some future date. The first inmates were moved into the first floor of
this four-level building on October 23, 2006. On February 27 of 2007, the remaining inmates who had been housed out to other agencies due to overcrowding were brought back
to Gwinnett and housed in the new tower.The original facility can hold up to 1,274 inmates, and the new tower has added 1,440 to the site, which included 102
specialty beds that are designed to hold maximum-security inmates, medical inmates and disciplinary inmates. The total capacity for the jail is now 2,744 beds. In 2008,
an additional 21 specialty beds were added to increase the total capacity to 2,765. The facility at present has a total population of more than 2,600 inmates.


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After spending a huge amount of money on buying your iPhone X, don't forget to protect it with a screen protector. Most of the people does this mistake of not protecting their mobile screen with protector and later on regret. Screen Protector is important to keep your phone screen protected against scratches, or from being broken. Therefore, it is always a good idea to immediately apply a screen protector on your iPhone X screen as soon as you buy it. Here are some of the best iPhone X screen protectors that you may consider for your iPhone.
- UAG Glass Tint
This 0.2 mm thick glass protector allows clear vision from the front while harder to see from sides. In this way, this protector makes it difficult for people around you to look into your phone. However, you can still use it effectively while giving full protection to your screen.
- Armorsuit Matte Screen Protector
This is another best screen protector for your iPhone X that is matte in nature. The biggest benefit of using this protector on your iPhone is that it cancels out the unsightly glare. As a result, you will better able to see your mobile screen, even in the day light.
- Full Coverage SPIGEN Protector
This screen protector is made up of 9H tempered glass that offers greater protection against drop and scratch. Furthermore, it comes with colored edges that add more aesthetics to your iPhone screen along with keeping it secure against damages.
- Cellularize Impact Protection
For iPhone X, this is another best screen protector because of multiple features. For example, it is grease absorbent for your fingerprints and allows you to continue using your phone without any issue. It also ensures protection, clarity, and responsiveness. You can find it here on their website, or on Amazon.
- Anker Glass guard protector
It comes with a slightly curved edge in order to provide enhanced protection to your iPhone's front screen. Furthermore, it ensures deep protection and comes with a proper installation guide. In this way, anyone can buy and easily install it paying anyone to do so.
- Supershields film-screen protector
This is a best screen protector for iPhone X if you work in a dusty environment. You will get annoyed when you have to clear the dust from your iPhone screen every time you use it for clarity. With this screen protector, the dust particles wouldn't stay on it while provide protection against any damages to your phone.
- Case-Mate protector
For iPhone X, this screen protector is also effective as it comes with 9H tempered glass. It also cancels out the shine or glare on your iPhone to enhance readability or screen visuals. Furthermore, it goes in long run due to its strong anti-scratch properties.
- Tech21 Evo Glass protector
It is another 9H tempered glass screen protector with 0.4mm thickness and high quality. The best thing about this protector is that Evo Glass gives lifetime guarantee for this protector. It will give full protection to your iPhone against any type of accidental drops as well as scratches.


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To get the car on rent is the best choice when you have no car but need it badly. For example, you want to go on a trip but your car is run out of order then it is the best idea to get the car on rent instead of waiting for public transport. There are a lot of car rental companies that let you car per hour, day, week or month. Even on android you can book the cars for trips, wedding, business and other purposes especially when you are in a foreign country or airport and looking for the convenient vehicle then car rental apps for android can become handy for you. These apps help you to quickly arrange any type of car and easily reach to your destination. Car rental apps will take less rent from you; provide you the complete car details, easy rental procedure, fuel services and much more. These apps also give you the complete guidance from where to take and return the car.
Below Newzoogle will discuss the best car rental apps for android. Let's check them.
Zoomcar Self Drive Car Rental
Zoomcar gives you more than 19 different vehicles and lets you to select your favorite one. Within minutes, the car will be on your door or nearby parking station. The price rates are affordable, free fuel, flexible packages, easy payment options and special offers are also included in this car rental app. If you have any problem during the drive then zoomcar will always there to help you.
Rentalcars.com Car hire App
This is a worldwide famous car rental app that informs you about the best car hire companies. It shows you the map to quickly go to the nearest car rental places and pick up the best car for your journey. You can easily cancel or book the cars on low rates. Rentalcars.com also supports 50 currencies, 32 languages and 6000 locations around the world.
Enterprise Rent-A-Car
Enterprise Rent-A-Car provides you reliable services to easily get the cars on rent whenever and wherever you want. You can quickly search the best car rental points that are located near to your area. You can get the car rental details, road side assistance and enterprise account access to easily manage the reservations.
Hertz RentACar
Hertz is an amazing car rental app for android that lets you to quickly reserve your favorite vehicle. You can easily locate the Hertz car station and book the cars in low rates. Once you signed into the Hertz's website, you will get all the car rental facilities, roadside assistance, pay now and later options.
Sixt Rent a Car
Sixt car rental app shows you more than 180,000 vehicles that are available in affordable rents across multiple countries. You can filter your search and get the car up to your favorite color, model and brand etc. With Sixt, you can easily get the directions where to take and drop the rented car. This car rental app also displays the rent rate according to your local currency.
Turo – Rent Better Cars
If you want to get the beautiful and good conditioned cars on rent then "Turo" is best for you. It enables you to meet the real car owners and make the live dealings with them. You will get 30% discount per rent and this is the main feature which make the Turo ever popular. You can also try this app to rent out your cars and make it the source to earn money.
Do you like any of the above car rental apps for android? Which car rental app provides the best services in low rates? Newzoogle is waiting for your feedback through comments and emails.


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One of the most common retail tools for collecting customer feedback is a "net promoter score." The concept of net promoter is simple. Customers are asked whether they would recommend the store (or service) to friends and family on a scale of 0-10 . A score of 9-10 is a promoter. A score of 7-8 is neutral. A 6 score or below is a detractor. Subtract the detractor percentage from the promoter percentage and you have a "net" number: the Net Promoter Score.
Promoters are customers who are enthusiastic about your store or brand and will keep buying from you. These are loyal customers who drive growth over time through their positive "word of mouth" marketing to their network. Neutral customers are currently satisfied but constantly at risk to switch stores or brands. Detractors are unhappy customers who can damage sales and give your store or brand a bad reputation throughout their network.
A net promoter score is useful for doing more than capturing your customer's feedback at one point in time. It is especially useful when comparing scores over time or across locations. Savvy retailers look at their net promoter scores during peak hours and non-peak hours. They compare those scores to understand the possible degradation in customer service during busy hours. They compare scores by floor manager to understand which ones direct and lead staff to provide excellent customer service and which do not. Most large retailers, restaurant chains and service providers use net promoter scores.
Customers are incented to provide a net promoter score by either going to a website or a toll-free phone number for a "less than one minute" survey. In return for participating in the scoring, customers could win a gift card or a discount on a future purchase. Customers are typically notified of the survey through register receipt messaging or from store associates directly. There are dozens of Net Promoter providers that can be found online. Many integrate seamlessly with the most popular POS platforms.


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Whether you plan to be out for a few days or a few hours, having critical supplies in a compact weather-resistant case is an essential part of preparation. Please see the "What's Included" tab for details on the kit contents—and we fully expect you to say to yourself (or out loud), "Wow, this thing really does have all the stuff!"
It's worth mentioning that this tin also features a handy LED lantern light.
Dual mode (static and SOS) LED 'flood' beam lantern light, illuminates a large area (batteries included).
Kit contents:
• Compass (oil filled)
• 4 hour pure Canadian beeswax candle
• Razor blade
• 6 Aquatabs® water purification tablets. Purify up to 3 gallons (each tab purifies up to 2 liters, depending on water quality)
• Wire saw (high tensile, 60lb working strength with handle straps)
• Aluminum beadless emergency whistle
• Waterproof matches
• Tinder Quik® fire starters
• Fishing Gear
• Signalling Mirror
• Marine grade rope (250lb breaking strength)
• Reflective trail markers
• P38 military GI-type can opener
• First aid supplies
• VSSL priorities of survival and instructions
One year limited warranty.


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DO-it-yourself ideas
Learn to create fun stuff at home, assorted by craft categories and more.Upload Your Own Creations
By sajan | Bangalore, Karnataka
Take the quilling strips and roll it tightly around the quilling tool and paste it using Fabric Glue as shown in the picture. Attach two set of quilling strips
2(for stud)
2(for lower step)
4(for middle step)
6(for top step).
Use a quilling mould and shape it as shown in the picture.
Apply Fevicryl No Stitch Fabric Glue inside the jhumkas and allow them to dry.Paint the outer side of the Jhumkas using Acrylic Colour Rose Pink to get an even tone .
Using Jewellery making tools, create the pair of earrings to finish the three step jhumkas.
Quilling Strips - 3 mm, Slotted Quilling Tool, Quilling Mould, Fevicryl No Stitch Fabric Glue, Fevicryl Pearl Garnet 318, Heritage Liquid Glass Varnish, Jewellery fittings, beads, stones


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Shock as bookseller dies suddenly
There is widespread shock and sadness locally this week at the sudden death of Portlaois-based bookshop owner John McNamee.
Mr McNamee, aged 59, who passed away on Sunday, January 4, was a familiar face to thousands of customers in his shop on Lyster Sq. He was always on hand to help with everything from schoolbooks to finding a rare edition.
He was also an active member of Laois Chamber of Commerce and Downtown Portlaoise, promoting the 'shop local' ethos. Portlaoise businessman Pat Miller spoke glowingly of his late friend.
"He was my good friend for 30 years. He was at the forefront of everything, dynamic in business with a huge interest in Laois GAA. We sympathise with his wife Betty and family and will do what we can to support them. He may be gone but we will certainly not forget him," he said.
From Lurgan, Armagh, Mr McNamee came to Portlaoise thirty years ago and set up Laois Education Supplies, later opening an Eason branch.
Among those he held booksignings for were the late Seamus Heaney and Albert Reynolds. He also championed Laois writers such as Jack Carter.
Mr McNamee was President of the European Booksellers Federation from 2005 to 2014. The EIBF said he "won the admiration and respect of everyone with whom he worked".
Reposing at his home today, Tuesday, from 3pm. Requiem Mass at SS Peter & Paul's Church 12noon on Wednesday, followed by burial.


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Introduction for Week 4
Welcome to the final week of the Advanced Machine Learning course. No video introduction this week, I am afraid - though it saves me looking at myself, which I am happy about!
This week we will start off by looking at feature engineering: How you can work with your original raw features to generate features that are more powerful when used in statistical modeling algorithms. In particular we will focus on principal component analysis (PCA).
After that, we will look at missing data and missing data imputation algorithms. These algorithms allow us to estimate values of missing data so that we can produce a 'completed' data set that can be used by statistical modeling algorithms. We will see that the expectation maximization (EM) and Metropolis in Gibbs MCMC algorithms can be used for this.
Finally, we will briefly look at some more complicated types of machine learning: Reinforcement learning and semi-supervised learning. We will examine an example algorithm in each case, and discuss what such approaches can do.
So well done for getting this far, and we hope you enjoy this last week!
© Dr Michael Ashcroft


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This cleansing milk's soothing formula respects the skin's natural moisture barrier and leaves it clean, silky and hydrated.
Morning and night, apply with fingertips to face and neck. Wipe off with a cotton pad or rinse with lukewarm water. Follow with Alcohol-Free Soothing Toner.
For all skin types.
I've used this cleanser for years, and it truly leaves my skin looking so much younger. The skin literally radiates after I use it. Really disappointed it's no longer at Rexalls and Shoppers Drug Mart in Canada. Will it return!? Please don't let it be discontinued!
I have been using this product for years and love it. I cannot find it any more at shoppers drug mart, London drugs. Is this product discontinued? I hope not!!!!
I use this to melt off my sunscreen . It works . Then I cleanse w my gel cleanser .
This cleanser takes off all my makeup - including my eye makeup - and leaves my skin feeling soft and hydrated. My skin is very sensitive, so this is a perfect choice.
For my sensitive skin, this has been the only cleanser that has helped calmed it down. When I use other cleansers, I experience occasional breakouts and this is the only cleanser that prevents them from happening. This has also removed these tiny bumps on my skin and my skin has never been so smooth! Please don't discontinue this product ever!
I used to use Marcelle yrs ago but they discontinued the product I used so I moved on to a different brand. I have again skin issues with breaking out into a rash which seems like roscea, I have tryed many cleansers that is suppose to be gentle but still irritate my skin. I then decided to go back to my old trusted brand Marcelle and bought this cleasning milk and i'm in love with this product! Thanks Marcelle!
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Operationalize your serialization requirements from product receipt through shipping and meet the massive scale required by serialization through TraceLink's purpose built, cloud-based solution. Quickly onboard and integrate your partners — ranging from pharmaceutical manufacturers, contract manufactures, distributors, hospitals and pharmacies — and meet their unique data and format requirements using TraceLink's Integrate Once, Interoperate with Everyone™ approach.
- Trade partner integration. Easily configurable connections with trade partners eliminate point to point integration risk.
- Data and operational scalability. On-demand and proactive scalability to ensure transaction processing and serial number lookups do not delay business processes.
- Operationalized processes. Pre-built business logic and configurations developed specifically for the unique processes of 3PL distribution.
- Manage serialization at the edge. Purpose-built mobile solution to drive serialization enabled receiving, pick, pack, and ship processes.
Serialized Operations Manager
Serialized Operations Manager (SOM) provides an EPCIS repository to manage serialization and related event data. Integrating with your WMS and ERP systems, Serialized Operations Manager ensures that the serialized inventory remains in sync with the physical inventory and that the right serialization information is captured from and transmitted to partners. All of this is accomplished at operational speeds so that business efficiencies are not impacted.
Smart Inventory Tracker
Smart Inventory Tracker is a mobile application—natively connected to the cloud—that combines serialization and fulfillment operations in the warehouse to meet both business and regulatory compliance needs. The application manages your warehouse data where the product is handled, enabling rapid response. Smart Inventory Tracker verifies and updates the status of the serialized product in your TraceLink serialization repository, Warehouse Management System (WMS), and Enterprise Resource Planning (ERP), with compliance reporting triggered based on configurable workflows.
Edge Track
Edge Track provides serialization capabilities at the edge. Rather than modifying an already heavily customized WMS or ERP, Edge Track integrates to your operational systems and provides the serialization software for handheld scanners and mobile devices. Edge Track ensures that your WMS, ERP and the Serialized Operations Manager are all operating in sync.


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The Finer Things
|Reckless Vermilion Onyx (3)
DescriptionThe Pandaria Treasure Society is interested in purchasing finely crafted goods from skilled jewelcrafters such as yourself. The items will be catalogued, and the most exceptional cuts stored in a secret vault, while the rest are sold to refresh our funds.
Our goal is to eventually create a unique collection for a special event.
RewardsYou will receive:
|Zen Jewelcrafter's Token
GainsUpon completion of this quest you will gain:
- 1 skill point in Pandaria Jewelcrafting
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The Wowhead Client is a little application we use to keep our database up to date, and to provide you with some nifty extra functionality on the website!
It serves 2 main purposes:
- It maintains a WoW addon called the Wowhead Looter, which collects data as you play the game!
- It uploads the collected data to Wowhead in order to keep the database up-to-date!
You can also use it to keep track of your completed quests, recipes, mounts, companion pets, and titles!
So, what are you waiting for? Download the client and get started.


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'This is Home' recognizes Riverside and Avondale | News
JACKSONVILLE, Fla. -- Riverside and Avondale are routinely recognized as among the great neighborhoods in Jacksonville and Florida.
It was even named one of the top ten neighborhoods in the country for 2010 by the American Planning Association.
Join us Friday at 6 a.m. on Good Morning Jacksonville to see why so many people love to call Riverside and Avondale home.


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Southwestern China and Sediments in Gulf of Martaban, Myanmar – October 23rd, 201116.7N 96.9E
Clouds hang over the tops of the mountainous terrain of southwest China, near the border with Myanmar (left). Many of these mountain ridges are in the province of Yunnan, a series of high mountain chains spreads across the province, especially in the north and west.
Visible to the south is the sediment-laden Gulf of Martaban, an arm of the Andaman Sea in the southern part of Myanmar. The Salween Sittaung and Yangon rivers empty into it. A characteristic feature of the Gulf of Martaban is that it has a tide-dominated coastline. Tides ranges between 4–7 m with the highest tidal range at the Elephant Point in the western Gulf of Martaban.


text: Posted in Bolivia 3 Comments Copacabana for Easter by Renee on April 21, 2016 Copacabana, Bolivia, is a small seaside tourist town- except it's on a lake. The horseshoe bay is a mix of sand and pebble with a flotilla of swan-shaped peddle boats awaiting passengers and dozens of boats moored in the bay. Most of the boats are 12 to 20 seater tourist transports, used for tours to the nearby islands. We travelled from La Paz to Copacabana by bus; a complicated process. Firstly, the ride through El Alto and the hour beyond was full of construction work and rough dirt road detours. Good news though, that means they're improving the road so in 6 months or so, it'll be a great highway! The view of El Alto from the bus Then there is the Tiquina straight. Although this is the main route to Copacabana and to the Peruvian border, there is no bridge across the straight. All passengers are ordered off the bus, need to buy a ticket and cross the lake in a series of small boats. Meanwhile, the bus drives onto a rickety wooden ferry, and with help of only a 40hp outboard motor, it makes its way to the other bank. The first issue is the driver only explained this in Spanish and the bus was 3/4 English speaking tourist. Some know-it-all (me) translated for the other passengers. The second issue was we just happened to be travelling on el Día del Mar. Michael and I were on the third boat to leave the dock for the town on the other side; dozens followed soon after. Our bus was the fourth to make the crossing. The driver honked the horn, while everyone tried to remember which colour bus was theirs. If you're not on board within 5 minutes, the bus leaves along with any bags you have in the cargo, no head count. From there, it was only a curvy 45 minute drive to our Easter retreat of Copacabana. The steep uphill walk from the main street to our hotel (pre-booked for once), made me regret booking the place while I was panting up the 45° cobblestone road. But once we got La Cupola, wow! Our room, overlooking the bay There was a manicured garden with hammocks and alpacas feeding on the grass. Alpaca gets a bit too friendly with Renée The buildings had Moorish domed roofs. Our room had a balcony overlooking the bay, a view you could see while lying in bed. And we discovered, through trial and error, the hotel restaurant had the best food in the town. But I swear, the room was not expensive. You wanna go mate? In Copacabana we really took it easy. The town is tourist driven, but the touts aren't pushy. We went for a stroll along the shore front, looked at the dozens of peddle boats and few kayaks sitting idle on the beach. Inspecting the goods The row of restaurants facing the bay were empty and unappealing, each with a tin shed for the kitchens and a tent filled with plastic stackable chairs to the front. Behind these were actual buildings, hotels and cafés, pumping a cocophony of musical styles to the rather empty street. The main street But… that was Thursday. Boats moored, waiting for the visitors to arrive The town exploded by Good Friday. Every single hotel was full. The beach was suddenly filled to capacity with tents, three deep, as far as the eye could see. People who came late even set up tents in the town squares, on cement. The town was filled to capacity by Friday afternoon Copacabana is the place to be for people from La Paz looking for a Easter getaway. More than that, it is the place for a religious pilgrimage. We sat in one of the many identical cafés on the main street, eating some bland, poorly cooked, barely edible meal, chosen from an identical menu to all of the neighbouring businesses. From there we watched the pilgrims arrive, looking exhausted, shuffling slightly, and with hiking sticks and backpacks. Yep, these folks had walked from La Paz. The hill, on which our hotel was near the base, is the final point of the pilgrimage. We walked it for something to do. There are crucefixes and statues of the Virgin right to the top of the hill. Despite bins everywhere, the pilgrims chose not to use them I watched the climbers stop and pray at each one, then continue up the steep steps to the peak. One thing that struck me was, if it's such a holy place, why is no one taking care of it. People dropped rubbish wherever, and rocks, steps, even the venerated statues were covered in "Pablo waz 'ere" style graffiti. And rather than being a quiet place to pray, women at the peak sold beer and icecream, the wrappers of which soon littered the holy mountain. Beautiful view of the bay though On the night of Good Friday, we went to church. Michael was afraid that as Atheists we'd burn, but nothing. People. Everywhere. We had heard there would be an Easter procession but weren't sure where it would go. So we went to the source. Quite a beautiful church, and the architecture is totally different to all the others we saw in Peru It was the most solemn religious procession I've ever seen, save for the miked-up guy with a guitar singing Christian pop from the back seat of a taxi. Procession ran through the square to the lake Then boom! We woke up Monday morning to empty beaches, no tents, and a spattering of international tourists. And other than an essential cruise to Isla del Sol, the Island of the Sun, that was our five days in Copacabana. Sunset at 3800m Share this:FacebookTwitterTumblrPinterestReddit


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In this article, I will lay out what I believe to be a solid set of five first steps for SEO optimizing your new WordPress site. Of course, there is always more SEO to be done, but these five steps are crucial for success. In my experience building dozens of WordPress sites, they aren't too time consuming either!
1. Pick a theme that won't hold you back
There are a lot of themes out there that, while "pretty," are total garbage from an SEO perspective. They don't allow for sufficient content that Google can index, and may have bizarre linking structures. Here's a great example of a theme NOT to chose if you want to succeed in SEO:
What's wrong with this theme from an SEO perspective? Well, simply put, there's no parseable text whatsoever on the page. So unless you have a boat load of links, search engines won't view it as an authority on… pretty much anything. Don't get me wrong, themes like these are great for personal or portfolio-based websites, but if the goal is maximizing your exposure in organic search, it's best to steer clear. You'd be much better suited with a theme that has plenty of space for text-based content and hyperlinks, like this one:
2. Form a content plan by polling your audience and create individual pages
This step is crucial. I've seen many businesses flounder in SEO by writing exclusively about what they know, rather than what their audience wants to read. Chances are you are friends with some folks that fit into your key demographic – just ask them what they are interested in right now. Then write up a page (or post) of at least 300-500 words for each of these. Try to avoid combining multiple topics onto a single page, because it often makes it more difficult to rank well in Google when splitting your effort between two or more topics.
For example, suppose a friend of mine wanted to create a website about house music, with the goal of eventually selling related merchandise once he had built up an audience. He polled his audience and found that many of them were confused about all the different subgenres of house music. So, he saw this as an opportunity to create individual pages about the different subgenres in order to capitalize on people looking these terms up. So he ended up with pages like:
What is "deep" house music?
What is "progressive" house music?
What is "tech" house music?
Having individual pages for these was a much stronger approach for SEO than having one page addressing "What are deep, progressive, and tech house?"
3. Install the Yoast SEO plugin
There are a lot of SEO plugins out there, some better than others. But the one I've come to trust has been the Yoast SEO Plugin. It's free, not prone to bugs, and effective. You can find and install it just by visiting the plugins section of WordPress, clicking "Add New" and searching for Yoast SEO. Then just click "Install Now" under Yoast.
What will this do you for? Well, to start, it will clean up your URLs and generate you a high-quality XML sitemap (you might need to check the box at the top of the XML Sitemap page to enable that). Overall, it works really well out-of-the-box, but I recommend browsing through each of it's options, as the exact setting to choose will depend highly on your website. If you don't understand it, just install it, enable XML sitemap creation, and forget it.
4. Make sure your homepage and key landing pages have enough text
So I've already touched on this twice, but it's worth talking about specifically. Simply put, your chances of ranking well are much lower if you have only a small amount of text on a page. Generally, we recommend having at least 500 words of text (including a given keyword at least 2-3 times) on a page that you want to make visible in the Google or Bing search results.
This is absolutely crucial on your homepage, but can benefit other pages too – including your category pages. This might take some fiddling with the code of your theme, and so if you're not up for that, don't worry about it. But if you have the programming chops, I highly recommend including at least 300 words of text on each of your category pages.
5. Write optimized title tags and meta descriptions for your key landing pages
Finally, using the Yoast SEO box that appears beneath the text editor in the page editing interface of WordPress, you can specify custom title tags and meta descriptions for your posts and pages.
For any page that you'd like to see appearing in Google or Bing, take the time to write a unique title tag and meta description. Here are some guidelines:
Title tags:
- Keep 'em under 70 characters.
- Include at least one keyword, preferably at the first start of the title tag.
- If you need to separate keywords, do so with a "pipe" symbol: | . For instance, a good title tag could be: Deep House Music | How to Make Deep House | HouseGuru2013.com. This includes two separate keyphrases and also the website name.
- Try to make them unique for as many pages as possible.
Meta Descriptions:
- Keep 'em between 70 and 155 characters.
- Include at least one keyword – doesn't matter where it appears in the meta descriptions.
- Include a call to action, like "click here to learn more about.." or "find out how you can maximize …".
- Try to make them unique for as many pages as possible.


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The whole conceit is about the "Opt-Out" generation of upper-middle class women who quit high-powered jobs to be SAHM's when the economy was booming, and about how if they're rich and well-connected enough they can get jobs now, but for not-as-upper middle class ladies it's much harder. And there's a lot of good/interesting stuff there: choice, empowerment, work-life balance, etc. But two things in particular stuck out to me.
One, when these women were making the choice to "Opt Out", a poll found that 40% of Americans believed that a mother working was "harmful to her children;" now 75% of Americans agree that working moms and SAHM's can develop equally warm and secure relationships with their children. So it was interesting to me to see how contingent on economics the acceptability of women working outside the home is: the 40% was an 8 point increase from 1994, and has dropped to presumably around 25% now that everyone needs jobs and they're scarce. Apparently in a "perfect" world (that is, not financially fragile), moms should be at home. But if they must work, they get a pass.
And two, a woman who was feeling devalued and frustrated at home took a job when her kids hit middle school, and this comment from her (now-ex) husband was presented remarkably uncritically: "I look back on it as the beginning of the end of our marriage," Eisel said when we talked by phone last month. "Once she started to work, she started to place more value in herself, and because she put more value in herself, she put herself in front of a lot of things — family, and ultimately, her marriage." I couldn't believe what I was reading. If you see your wife "placing value in herself" as a negative, then her job wasn't the beginning of the end of your marriage; your attitude was. Also, he worked the entire time they were married and apparently he doesn't see that as putting himself "in front of" the family and marriage — it's just the way things work!
Anyway, lots of food for thought, but I'm worried it's going to be boiled down/contorted into more Mommy Wars BS.


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Budget $20-100 USD
build and redesign restaurant project.
Skills: Website Design
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Project ID: #1898
as we talked and as i did ------> Abi
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Map of the local innovation industry.
Meet some of the best and newest startups based in Brisbane.
QR codes systems for COVID-19.
Simple tools for bars, restaurants, offices, and other small proximity businesses.
Connect with Sale and Lease Opportunities
As Asia-Pacific's leading search portal, CorporateRealEstate.com specializes in the Corporate Tenant, Corporate Owner-Occupier and Corporate Investor markets. Its mission is to become a global online resource that enables corporate tenants and investors to access ...


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Several years ago during introductions in a beginning rider course a student mentioned that he was hoping to learn how to lay a bike down to avoid a crash. My response was "Laying down the bike IS crashing!!"
When a rider states they deliberately laid down a bike to avoid crashing into something it may
be an indication that the rider's physical and mental skills need improvement. The root cause is often improper swerving or [maximum] braking technique – two very important crash avoidance skills.
If they are new techniques to you, they may be skills best learned in a rider education course. I'll take a brief look at these two skills, and then bring a guest contribution on total stopping distance from Larry Buck; a friend and rider coach from Boca Raton, FL.
Swerving is probably the most effective maneuver for a quick response to avoid a hazard. The net effect is to put the motorcycle in a position to go around the hazard while remaining on the general path of travel. A swerve is two short aggressive counter-steer turns, the first to avoid the hazard and the second to get back on the path of travel. During a swerve the rider's body position should be independent from the motorcycle and upright. No brakes should be used during the swerving process as most available traction is used by the swerve process.
Maximum braking is typically defined as full application of both brakes without locking either wheel. The front brake provides the bulk of the braking power. Maximum braking is accomplished by using a firm, progressive squeeze of the front brake lever. This allows proportional weight shift to the front tire to maintain adequate traction while braking. Rear brake usage may be lessened as weigh shifts off the rear wheel.
Let's take a look at how the mental skills of good situational awareness and visual perception ties into good overall braking. The concept of total braking distance is not new. The remainder of this article comes from my guest contributor Larry Buck…
"Total stopping distance is comprised of three components – Perception Distance, Reaction Distance, and Braking Distance.
"Perception distance is how far you travel before you become aware of a hazard. The higher your speed, the more distance you travel while you perceive situations.
"Reaction distance is the distance traveled from the time you become aware of a hazard until you apply the brakes – higher speeds equal more distance traveled.
"Braking distance is the distance you travel after applying the brakes; higher speed equals more distance required to stop the bike. As an example, a typical total stopping distance from 60 MPH is 300 feet or more. Stated another way – a football field.
"You may have read that a motorcycle can stop in 150 feet from 60 MPH. How does that number relate to YOUR braking skills? The answer for most of us is very little. Remember that distance was done by a highly skilled rider, one who does quick stops every day.
"Do you do or practice quick stops every day? When the time comes when you need to stop – have to stop NOW, could you do it? Chances are probably not. Why? Because most riders do not practice doing quick stops, so when we really need to, things may not work out well.
"How often do you take some time to self-assess, and hone your braking skills? We all strive for the perfect line through a curve, a smooth shift, or a tight U-turn, but probably do not put the same effort into practicing stops in shorter distances.
"Knowing the above information, which of the 3 components of total stopping distance (perception, reaction, braking) do you think is most important? We can pretty much eliminate Reaction Distance because everyone reacts in approximately the same amount of time: one second. So that leaves the other two components; perception distance and braking distance. Of those two, which one do you think is more important?
"Let's assume that professional's 150 foot stopping distance equals a 10/10. The sooner you perceive a hazard, you may not need a 10/10's stop, maybe only a 9/10's or 7/10's stop. One of the ways to focus attention while riding is to play a "What If" scenario like an endless loop tape through your mind. By doing something similar, you will be perceiving situations sooner. This will provide you with space, and a strategy of how you will deal with the situation if required. Perception skills (your eyes and mind) will enable you to respond smoothly, instead of needing to instantly react."


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The cost of hiring the hall for private events is £20.00 per hour. It is particularly suitable for children's parties, wedding receptions, exhibitions and displays, large meetings, dance, exercise and other activities. It is a well-maintained, light, open and flexible space, approximately 13 x 8 metres, with a wooden floor. At one end of the hall there is a raised and curtained stage with a baby grand piano. There is a modestly-sized kitchen with a generous supply of crockery and cutlery, two fridges, an oven with an induction hob, and a microwave oven. The kitchen has a service area which can be opened up to the main hall. Tables and chairs can be provided: there are 9 large tables seating 6 comfortably, and 8 small tables seating 4 people, plus 84 chairs. The hall can hold 120 people. There are separate male, female, and disabled cloakroom and toilet facilities, including a folding baby-changing table, which lead off from the entrance lobby. At the rear of the hall there is a small, carpeted room which is also available to hire. The hall is surrounded by a large grassed area and there is space available for parking.
- We regret that we are unable to take any further bookings for 2020.


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The Cincinnati Workers' Rights Project serves terminated workers who made less than $20 per hour or $40,000 per year in their previous employment.
We offer our service to terminated employees through:
If you are a low-wage worker who has been terminated and you have questions about your rights and responsibilities, please call the Cincinnati Workers' Rights Project at (513) 592-2318.
Our regular office hours are Tuesday, Thursday, and Friday morning.
In addition to educating and counseling individual employees, the Cincinnati Workers' Rights Project is dedicated to advocating for systemic changes which will improve the working conditions and pay for all workers in the Cincinnati area.
The Cincinnati Workers' Rights Project is an IRS 501(c)(3) organization (application pending).
Courtney Jaconette
Courtney is the primary contact for the Cincinnati Workers' Rights Project. In the role of Executive Director, Courtney gathers information from employees and provides that information to an attorney who will be able to help. The volunteer attorney provides the employee with legal advice and helps the employee better understand his/her rights.
Workplace Fairness is a non-profit organization working to preserve and promote employee rights. workplacefairness.org provides valuable information for employees interested in better understanding their rights.
The Cincinnati Workers' Rights Project's Mission Statement
To offer quality legal advice and education and limited representation from an experienced employment attorney to low wage workers who have been terminated, as well as referrals to volunteer attorneys who will evaluate cases for litigation in appropriate circumstances, and to advocate for legislation and social change which will improve the lives of all working Cincinnatians.
The Cincinnati Workers' Rights Project's Values Statements
The economy must serve people, not the other way around. To that end:
The Cincinnati Workers' Rights Project values just and living wages for all workers in exchange for their labor.
The Cincinnati Workers' Rights Project will advocate for systemic changes, which will promote these values.
If you would like to speak with us regarding your employment rights and responsibilities, or a current employment-related situation, please submit the form below.
The Project needs volunteers and tax deductible donations to achieve its mission. Please consider helping with a gift of your time or financial contribution.
Contact us by phone or email to discuss volunteering opportunities.
(513) 592-2318
To donate, call us at (513) 592-2318 Or mail your donation to: Cincinnati Workers' Rights Project 600 Vine Street, Suite 900 Cincinnati, Ohio 45202


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Father's Day
Father's Day is almost here! Help dad cultivate closeness with God.
Father's Day is almost here! Help dad cultivate closeness with God.
In Seven Men, New York Times best-selling author Eric Metaxas presents seven exquisitely crafted short portraits of widely known but not well understood Christian men, each of whom uniquely showcases a commitment to live by certain virtues in the tr... Learn More
Tony Evans
Tony Evans
Alan P. Medinger
Mark Batterson
Shaunti Feldhahn
" Shaunti and Jeff have unearthed a treasure chest of insights eye-opening and life-changing. "
Andy Stanley, senior pastor, North Point Community Church
"Finally, you "can" understand her
If you're like most men, you've burned up lots ... Learn More
Stephen W. Sorenson
If matching wits with an elusive elk, chasing an antelope, or hunting pheasants excites you or someone you love, "I d Rather Be Hunting" is perfect Longtime hunter Stephen Sorenson shares memorable hunting stories and occasional misadventures to en... Learn More
Gregory Jantz
Steve Chapman
Steve Chapman's popular "A Look at Life from a Deer Stand" (more than 280,000 copies sold) now includes new stories and illustrations as well as a stunning new cover.
From the incredible rush of bagging the big one to standing in awe of God's ma... Learn More
Patrick M. Morley
The Man in the Mirror has established itself as a cornerstone in men's literature since its 1989 release. Winner of the prestigious Gold Medallion Award and appearing on the bestseller list eighteen times, it has helped thousands of men understand t... Learn More
In order to serve you better and ensure that you will get Canadian tax receipts for donations and the best shipping rates for purchases, please visit our Canadian store.Yes, take me to the Canadian site Continue to the U.S. Site
Thank you for your support!


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K&L Email Alerts
Sign up to receive custom alerts, new arrivals and the latest happenings from K&L Wines
Updating Your Billing, Shipping, And/Or Newsletter Address Information
Updating your address information is simple here at klwines.com. Go to Your Account and click on "Address Book" (you may have to log in with your Email address and Password). From there you can edit an existing address by clicking on it. You can also enter a new address from this area of our web site.


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The fields of geology and mining aren't typically associated with the term "online" but the world today allows mining professionals to access a wealth of knowledge at our fingertips (assuming your mobile coverage works!). As more and more professionals rely on the internet for information, we're providing you the top 10 resources for mining and exploration geologists.
1.Google Earth (google.com)
What better way to learn about the Earth than by viewing high-resolution satellite imagery of the entire planet? Download Google Earth Pro and start exploring. It's easier than ever to transfer GIS data into Google Earth along with downloading public geological data. Keep an eye out for our Mineral Exploration using Google Earth™ guidebook coming soon!
2.MinDat (mindat.org)
Mindat.org provides an excellent resource for information on minerals, rocks, gems and even some mine sites. Use their Advanced Search feature to search by location, mineral properties, chemistry, or the mineral name. Whenever you're at a loss when someone asks "what's in this rock?", MinDat is a great resource.
3.U. S. Geological Survey (www.usgs.gov)
It's hard to beat the USGS for free geological information. The USGS has been a premier global resource for all-things-geology for years, with emphasis on North American data. They have more than just maps though, you can easily get lost for hours in world class information on Mineral Resources, deposit models, and more.
4.Geology.com (www.geology.com)
A good source for all things geology-related. Geology.com doesn't cover just one discipline within geology but does a nice job of providing information on a wide range of subjects. Check out their dictionary of geological terms as well. It can be a handy reference when reading articles to look up some obscure terms.
5.Infomine (infomine.com)
Infomine is a Vancouver-based, mining-focused website providing a vast amount of information, news, and a good job board.
6.RSC Mining & Mineral Exploration (rscmme.com)
RSC has created a great online tool that allows you to review exploration and Mineral Resource data globally with helpful searchable filters. Not only can you view properties and mine sites on a map, you can also download the associated public reports such as NI43-101.
7.Professional Mining Organizations
Yes, the same professional organizations that require membership fees also provide a good deal of free information and references on their web sites. These include:
- Australian Institute for Mining & Metallurgy (www.ausimm.org)
- Canadian Institute of Mining (http://web.cim.org)
- Society of Mining, Metallurgy, & Exploration (www.smenet.org)
- Southern African Institute of Mining and Metallurgy (www.saimm.co.za)
This is the one online resource listed here that's not free unless you're already a member of a professional organization such as the ones listed in #7. Once logged in, OneMine.org allows you to read and download thousands of technical publications from hundreds of journals and organizations at no additional cost.
9.Wikipedia (wikipedia.com)
This is the same Wikipedia where you can waste hours reading random historical references or your 11-year-old plagiarizes their homework assignment. Being the largest encyclopedia in human history has its advantages though and it contains a lot of general geology, chemical, and mineralogical information.
10.Mining Geology HQ (mininggeologyhq.com)
We couldn't make a list of top online resources without including ourselves! Perhaps that's a bit self-centered but check out the Resources drop-down menu for helpful references on a variety of mining geology subjects including overviews on deposit types, geostatistical training, and helpful publications to download.
Geology, Exploration & Mining: 101 Exceptional Online Resources
Geology Online: 105 Sites That Rock


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Craig Biggio
Sponsored By
Johnson, Martinez, Smoltz, Biggio Enter HOFThree pitchers who became dominant after trades and a rock-solid catcher-turned-second baseman have a new moniker — Hall of Famer.
Astros Draft Sons Of Biggio & ClemensThe Houston Astros are bringing another Biggio on board. Another Rocket, too.
Biggio, Johnson, Martinez, Smoltz Elected To Hall Of FameRandy Johnson, Pedro Martinez, John Smoltz and Craig Biggio were elected to baseball's Hall of Fame on Tuesday, the first time since 1955 writers selected four players in one year.
No Players Elected To Baseball Hall Of FameFor just the eighth time in history, no eligible candidates received the required 75 percent of votes to gain entry into the Baseball Hall of Fame.


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Visit our Services Directory - The services directory is a one-stop-shop to search for services such as how to apply for a driver's license or medical assistance.
In partnership with the Minnesota Department of Human Services, the Olmsted County Financial Assistance Office works to help people with low incomes transition to economic stability. Economic support includes cash assistance programs, food support, child care assistance, child support services, refugee services, employment services, and health care programs.
If you already have a user ID and password, you can log-on to ApplyMN directly.
Apply Supplemental Nutrition Assistance Program (SNAP)
at https://applymn.dhs.mn.gov/online-app-web/spring/public/process-login?execution=e1s1
The SNAP Program (formerly called Food Stamp Program or Food Support) is a county-run, federal program that helps low-income households get supplemental food needed to attain sound nutrition and well-balanced meals. For more information about SNAP.
Apply General Assistance (GA) at https://applymn.dhs.mn.gov/online-app-web/spring/public/process-login?execution=e1s1
This is a state-funded supplement cash assistance program for people who receive Social Security Income (SSI) benefits. For more information on MSA.
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Pottery jar
Pottery jar from the Inca people of Peru in South America. The different cultures of Peru had a highly developed pottery technology. The Spanish colonised Peru and many of the local people were taken as slaves to work in mines.
Copyright: Copyright BCC Museum
Object ID:Ea6709
Beaker from Peru
Silver beaker made by the Chimú people, of Peru. The Spanish who colonised Peru were attracted by its natural resources, such as silver.
Copyright: Copyright BCC Museum
Object ID:E 1577
View of the Avon by Samual Jackson
Painting View of the Avon, by Samual Jackson. A rowing boat is being used to unload a ship on the River Avon near Bristol. The ship has probably come from the Caribbean. This painting shows turtles being unloaded.
Creator: Samual Jackson
Copyright: Copyright BCC Museum
Object ID:K4
Page 1 of 1 pages


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Rachel beats Miss V8 rivals
THE chorus of cheers can be heard from breakfast tables all over the Sunshine Coast as Coolum's Rachel Borosak was named the region's Miss V8 Supermodel winner.
The 18-year-old business student and part-time model won the Century Batteries search for the Coast's entrant from 17 other competitors at the Sands Tavern last week.
While not a die-hard fan of the road race series, Rachel knows more about cars than most women her age.
"It's really an opportunity for me to combine my interests in marketing with that of my modelling," Rachel said.
"I was really surprised that I actually won because there were a lot of great girls."
Rachel may look like a girl who has grown up near the beach, but she is only relatively new to life by the ocean.
"My family moved here about four years ago from Brisbane, but I feel like I've lived here all my life," said the girl who was also a Zoo Magazine competition finalist this year.
"I think it is good publicity to get my name out there and to get some confidence and some experience."
The national final will be held at the Gold Coast in a few weeks and will involve a week long series of interviews, public promotion activities and modelling assignments.
"I think I've got a pretty good chance, my strength is the interviews but I'm happy to just enjoy the experience."


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Choke is a common affliction related to the horse's eating habits. The first time you see a choking horse, you probably will get quite a scare. The horse may be frightened too. Choke occurs when a bail of food sticks in the esophagus, the tube leading to the stomach. Try as it might, the horse cannot get the feed all the way down. Saliva continues to be produced, and the horse drools excessively. You may see green feed coming out of the nostrils.
The horse may panic, throwing itself about and trying to rid itself of the annoyance. Or it may eventually stretch out its neck, drooling all the while. remember, though, the horse can still breathe. The food is lodged in the esophagus, not in the windpipe. When your horse chokes, your first reaction should be a calm one. Your horse can still breathe since there is no blockage of the airway. Keep your horse calm and prevent him from injuring himself by slowly walking or by standing with him and talking calmly. Sometimes you can see a lump on the side of the horse's neck where the ball of food is stuck in the esophagus. You may try to massage the throat area to help move the bolus of food down.
Choke usually occurs because the horse doesn't chew and lubricate his food properly before swallowing, so the food cannot pass normally. A greedy eater is the usual victim of choke. Also, horses that are fed in groups may eat faster than usual due to competition for feed, and so suffer from choke. Horses trying to eat in a swaying trailer can choke when they accidentally swallow a bolus of food before they are ready. Finally, the horse that is overdue to have its teeth floated is a prime candidate for a choke episode; the feed just can't be chewed normally. Most cases of choke can be prevented. If you feed horses in a group, provide several feeding areas to reduce competition for feed. Avoid feeding grain in a trailer, since grain is more likely to cause choke than is hay (do not provide any feed in the trailer to a horse that gobbles its feed or has a history of choking).
The greedy eater is the horse that is most difficult to protect from choke. Several methods have been tried, and one may work for a particular horse but be useless for another. Feed hay before grain, so the horse is slightly full and less likely to bolt the concentrated feed. Place large rocks in the grain bucket, forcing the horse to pick its way around the rocks to eat the grain. Finally, spread out the feed over a large area to prevent the horse from gulping it down. Yearly dental exams ensure that the horse's teeth are in good shape and can perform their needed function. Horses with teeth in very poor condition can be given specially prepared feed.
Feline Immunodeficiency Virus
Feline Bordetellosis
Feline Distemper
Feline Infectious Anemia
Feline Infectious Peritonitis


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- motherly
- nurturing
- not too girly
- sense of duty
- soft tenderness
- calm personality
- not easy nor hasty
- unquestionable trust
- has own favorite things
- serves a higher purpose
- not needlessly transparent
- not desperate for someone
- does things slowly with care
- warm without being flirtatious
- secure enough to not be clingy
- could look into your eyes forever
- has a great purpose to fulfill in life
- not overly obsessed with anything
- spontaneous without being careless
- down-to-earth and genuinely original
- values a small meaningful social circle
- not overly concerned with other people
- ideally has similar build and activity level
- likes to have fun and equally likes to work
- has enough attitude to excite and laugh with
- has common sense and practices moderation
- makes efforts to look nice without over doing it
- she makes an effort to learn about your interests
- does not always feel like she has to wear makeup
- someone who you could keep loving unequivocally
- fatherly
- masculine
- listens to you
- grows with you
- layered strength
- deeply hears you
- incredibly thoughtful
- never breaks your trust
- sensitive to all your needs
- remains solid in his choices
- confidence in knowing himself
- unwavering in purpose and goals
- attuned to your feelings and desires
- he does not wait around or play games
- he cherishes you and cannot get enough
- he is always clear about what it is he wants
- he deliberately chose you and only you forever
- he is the man he wants to be before he meets you
- when he does something you know he will do it well
- has deep-rooted humility while being relatably imperfect
- has strong principles without being overly proud of himself
- he is in no rush whatsoever and takes him time with everything
- a man that does not need to control but is willing to take charge
- he puts you first everyday and shows you are worth his attention
- has strong beliefs without being judgemental towards other beliefs
- simple at his core who values what he has and doesn't need excess
- most importantly he is genuine and never apologies for being himself
- someone who you could keep loving unequivocally and without reserve
Almost the Answer to Finding Love… (You didn't know you were searching for.)
Firstly… Find love in your life… don't find love and then search for a life.
That being said… not all of us are capable or willing to sit idle and wait for a storybook entrance of love into their life in some romantic swoop… but, hey, you never know! 😉
So, what is the only possible answer to finding love, you ask? Oh, glad you asked… KNOW WHAT YOU ARE NOT LOOKING FOR! It may seem like a ballsy statement that can't possibly be helpful or must not be true for so many people, that will say, they don't know what they are looking for (or not looking for) until they find it or don't find it… But, please bear with me and take a moment to consider how many people never really get clear, like really clear about what they know they don't want.
Change your mind about what you know you don't want, that's totally fine… But, try to get really clear about the new thoughts of what you are thinking you know you don't want.
This is B.S you say, ok… well, that is a valid opinion… but, I do want to point out that the title is about finding love, not getting lucky enough to fall into love with someone that luckily turned out to be a great fit for your life. This is a reminder of not compromising yourself and be confident in finding the love you want for the rest of your life or in your life, period.
Desperation, compromising on your needs and dreams, can too easily turn into tricky and convenience self mind games that eventually somehow make a wrong fit seems like a perfect fit for the love you've been searching for… for so long. SO, write down, scrapbook it, bookmark or by all means save it somehow where you will remember it, so you will have a reference of what you are not looking for… and if you happen to know (or even think you know) what you are looking for, write that down too.
Love in Religion
If you read about every major religion in the world, there are strands of religious beliefs and traditions that all have a similar message of love. Love through compassion, love through forgiveness, love through belief and encouragement, love through patience and honesty, love through happiness and contentment, as well as love through various other virtues and disciplines. If love doesn't unite us all, then I don't know what does.
"Love is the answer…" — John Lennon
Mutual love, mutual life, mutual respect, mutual dependence, mutual learning, mutual admiration, mutual co-existence… Mutualism is about harmony.
10 Reasons You Should Keep a Love Journal
"Keeping a personal journal a daily in-depth analysis and evaluation of your experiences is a high-leverage activity that increases self-awareness and enhances all the endowments and the synergy among them." — Stephen R.Covey
- It's completely doable in just a few minutes a day, even the most busiest and stressed person can spend at least a few minutes a day on something that could greatly improve their most important relationship in their life.
- It allows you to clear your emotions that have been building up all day and putting them out there into words, can also bring a lot of clarity to what emotions you were experiencing and what may be behind those emotions.
- It can work like a free personal therapist, in that you can unload and get feedback from your significant other, when you are ready to receive input and discuss what happened or what went on internally for you.
- It can help give you a sense of direction, since you will be able to track and review your personal love history, which can help you learn about yourself quite a lot.
- It gives you a medium to freely express yourself, which the pressure of being in someone's face about possibly very sensitive issues. Sometimes you want to say something, but that doesn't necessarily mean you are ready to discuss it. This also gives the option for you to pass along a page from your love journal to your partner with the understanding that you aren't quite ready to have a discussion, but wanted them to know.
- It can increase gratitude, both for yourself and for the other people whom you allow to read it… Being human isn't easy and when people can read about your feelings and thoughts at their own pace to fully digest. It also allows them time for self-reflection, which can light-up compassion, gratitude and empathy in their hearts.
- It can improve your writing, as in, building the skills that come along with taking your thoughts and turning them into words. As well as, developing your thoughts, so that they can be effectively communicated through words… wether read or spoken.
- It can power-up your creativity, just like anything you create and pour your heart into, it can open-up facets of your being that open new possibilities to ways you can get creative and contribute to the world for the betterment of humankind.
- It can allow you to gain more self-awareness than every before, especially if you take a little extra time to go back and review your previous love journal entries. Being aware of something is the first step before you can improve upon that something.
- It improves your productivity, yeah, that's right… you are producing something and that can have quite the synergistic effect on your life. You may just find that you get more productive in other parts of your life as well. It always help to have small daily accomplishments to keep your life moving and the energy flowing.
Sustaining Love
Before a person begins to focus on how to sustain love in a relationship, they should be sure that the love is one worth sustaining: a wholesome love, a fearless love, a supportive healthy love, an unrestricted and unrestrained love. Your relationship may not be everything you want it to be all the time, and that's perfectly fine, but ask yourself if the love is worth sustaining. If it is, then be prepared to put in the effort.
Love is simple, but not easy.
Love is all you need and all you need is love… simple right?
Live-and-breath love… love yourself in every breathe, love others and the world around you. Simple.
Live your life through love… pour your heart into everything you do, feel the love from others.
Simply love everyone and everything that you put your attention on… and your life will be filled with love.
So… what's not easy about that… what gets in the way…
Love the Best You
Being the best you may seem impossible or a fairytale, since all the parts of you may not seem like even close to the best parts (to put it mildly.)
The best you will always be you doing your best.
What you do best, may not always allow you to be you at your best.
You at your best is not a destination, it is a winding path towards what's in your heart.
Loving you at your best, is not loving what you do or accomplish, but loving who you are and where your mind, heart and spirit are. Becoming the best version of yourself does not require anything around you to change and only consists of you becoming the best you, you can be.
Motivated by Love
At the most basic level of why we do what we do… we are all motivated by love.
We are social beings at are core and even if we are hardcore homebodies there is still a intrinsic part of ourselves that was nurtured and wants to be nurtured… and in turn wants to nurture or give something.
That being said, many (if not most), operate in a mode of extrinsic motivate, be it fear or reward… which is far less powerful than an intrinsic motivation of love and contribution. The employee that gets enough to cover the bills and have a little fun whom is motivated by reward, looks forward to getting off work to go play. But, the employee that believes in what they do and feel like they are perhaps making the world a better place, could find themselves working long past the standard work day to go above and beyond.
If nothing else we can be remembered by the sum of our contributions to humanity.


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About The Wristband Company
Based at Fir Tree Farm in the Cheshire countryside The Wristband Company is a leading supplier of event and promotional wristbands, with over 6 years market experience.
Our aim is to offer a fast, friendly and reliable service and to be on hand to answer any queries or questions you may have.
We are a young and vibrant team and try and keep the company up-to-date with all the developing technology. No enquiry is too big or too small and we are always on hand to offer a helpful answer so please feel free to contact us.
I graduated from Lancaster University with a 2:1 in Business Studies and, after gaining market experience, I have decided to set-up The Wristband Company. I like to think that the culture of the company is that it will always be a friendly, fun place to work whilst I am determined that we will offer the highest quality service in the market. In my spare time I enjoy playing sport, socialising with friends and I regularly attend gigs and festivals.
My football team is Crewe Alexandra and top 3 gigs are The Courteeners, The Enemy and The Pigeon Detectives. You can keep up-to-date with all of the office activities by following us on twitter and facebook.
Having previously worked for an asset management bank I am now ready to throw all my energy into the world of wristbands. My previous experience has taught me how important it is to take a client focused approach, which I aim to continue for The Wristband Company.
I am an active individual, whether this be playing football for Stretton Young Farmers or going to the gym. My football team is Wigan Athletic, although I might have to swap if they carry on playing like they currently are. Socialising and music both play an important part of my life, which is why I am currently learning the guitar and forming a band.


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An In-Depth Look at our Preschool Curriculum
The Safari Kid curriculum contains four levels for preschool children. Each level contains the skills necessary for kindergarten preparedness. Students will begin at Level 1. After two weeks, an assessment will be performed, and if the child is ready, they will be moved to the Level 2 curriculum, and so on. Every child is encouraged to work to their best ability.
Preschool Level 1
This level introduces students to the alphabet, phonics, basic science concepts, and basic number sense. We use Booklet Practice to supplement these subjects. Story Time fosters emergent language skills. During the day, kids have repeated opportunities to work on gross and fine motor skills, creative arts, and music and movement.
Preschool Level 2
Level 2 is based on our Safari Kid Thematic Approach. Class participation enhances social/emotional awareness and the modeling of appropriate classroom behavior. We support all levels of kindergarten preparedness with our curriculum. Our morning routines are designed to encourage participation and boost confidence with public speaking.
The Safari Kid Booklet System provides opportunities to practice the concepts of number sense, basic mathematical concepts, and spatial knowledge. We introduce vowels, sight words, and early language concepts. Scientific inquiry is supported by our STEAM curriculum, and there's always plenty of time for creative arts, physical play, and fun and free exploration.
Preschool Level 3
Our focus moves towards advanced phonics, reading comprehension, mathematical concepts, and spatial and temporal knowledge. Scientific exploration with hands-on STEAM activities encourages natural curiosity and inquiry. Of course, we integrate play into our learning to enhance learning, imagination, and the sharing of ideas. Music and movement, TEMBO, block play, dramatic play, and more are a part of every day at Safari Kid!
Preschool Level 4
Children in this class are reading at the kindergarten level. Our special morning routines and Booklet System foster critical thinking, and an emphasis is placed on skills for lifelong learning success. Students continue to pursue hands-on scientific inquiry, language development, and increased self-esteem. Free play is always part of the Safari Kid day, both in large and small groups.
Questions? Contact the Safari Kid near your location to learn more!
About Us
Safari Kid is an International Chain of Preschools and After School Programs, founded by Ms. Shy Mudakavi in the heart of Silicon Valley in Newark, California. Over fifteen years, 35,000 children going through the program have shown consistent excellence in elementary and middle schools.


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How to stay healthy over the holidays
By Mara Shapiro
December 12, 2013
It can be difficult to find the motivation to eat healthy and stay active during winter. The eggnog and pies are calling, and mom's cooking does not help either. However, it is important to maintain a healthy body even when all we want to do is pig out on holiday treats and watch Netflix. Erica Ne...


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Facebook launches verified pages and profiles to help users identify authentic accounts
Facebook today announced the launch of verified pages and profiles, which will make it easier for users to find official fan pages and accounts for top brands, celebrities, government officials and other public figures.
Like on Twitter, verified accounts on Facebook will be denoted with a small blue icon with a checkmark in it. It will appear next to the user or page's name on their Timeline, in search results and elsewhere around Facebook.
For now, users and page owners cannot request to have their accounts verified. Instead, Facebook is proactively verifying the pages and profiles with the largest audiences.
Although Twitter has had verified accounts for years, Facebook did not publicly indicate which pages or profiles were official until now. The social network did begin authenticating accounts with a lot of subscribers last year, leading those accounts to appear more often in "People To Subscribe To" recommendations on the site, but there was no visual indication that a profile was official.
The company appears to be preparing for another push to get celebrities on the platform by expanding its strategic partner development team to build relationships with athletes, musicians and other entertainers. Facebook is also likely to be incorporating hashtags into its service in the near future.
Although users cannot request to get their page or profile verified, Facebook says they can report other pages or profiles that impersonate them.


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A Powerhouse of Humanitarian Science and Diplomacy
for Over 75 Years
MGIMO University remains the leading Diplomatic University in Russia and continues to expand its list of international partner Institutions and Organizations.
The Odintsovo Campus has already established cooperation with several Universities since 2016 (a full list can be accessed at this link).
The 2020 Summer Program will be the most wide reaching and challenging Program we've designed so far. It will provide our students with the necessary knowledge to bridge the gap between Russian Foreign, Domestic and Energy Policy - giving them a deeper understanding of the contemporary geopolitical situation in Europe and the world, and providing insight into the inner-workings of Russian policy making. Our staff of acclaimed university professors, prominent politicians, experienced diplomats and renowned experts have developed a directed and cohesive program, which tackles all the most important aspects of Russian Foreign Policy. Students will attend daily lectures and seminars, take part in group projects and presentations, and attend guest-speaker events - all of which will be conducted exclusively in English.
Henning Sasenrath
Excellent speakers giving insight into a vast spectrum of opinions and ideas, extraordinary excursions into key insittutions, finally the feeling of exhaustion in the best possible way: I strongly recommend participting in the summer program!
Lina Gervazieva
One of the most memorable milestones of my education was realized during last summer's MGIMO program, which included a Russian Foreign Policy module. I gained experience in forecasting international processes and understanding the historical trajectory of governments around the world. Now, the other members of the program are my friends, we still communicate online discussing our experiences. I truly recommend this summer program!
Caleb Chia
The benefits to one's personal networks and one's understanding/appreciation of the world and other cultures are off the charts. Further, MGIMO has built into its curriculum for their summer school participants an entire itinerary of various sightseeing activities. The rigor and intensity of the academic curriculum at MGIMO was well paired with the richness of the cultural and recreational experiences on offer.
will be held at the new MGIMO University campus in Odintsovo, in one of the most prestigious suburban Moscow areas - "Rublevka", where the President of Russia and most of the Federal ministers reside. Five minutes from Moscow by the new Northern Bypass Highway and just 20 kilometers from the Kremlin and Red Square, the modern campus is notably in an eco-friendly, healthy country-side environment. At the same time, the campus remains completely accessible thanks to an efficient transportation system, which will bring you to downtown Moscow in no more than half an hour. Summer Program students will reside at the "MGIMO Hotel" in Odintsovo, an officially registered 3-star Hotel attached to the main building.
Currently enrolled University Students, Post-Graduate Students, Professionals in the fields of International Relations, Russian and European Foreign Policy, Russian Energy Policy and International Energy Cooperation. Moreover, the Summer Program is balanced in such a way that it will be equally interesting to individuals without deep background knowledge in the aforementioned topics. Students that are interested in learning more about Russian History and Culture, that want to gain more knowledge about contemporary Russian policy-making, see Moscow and the surrounding region first hand and improve (or begin to form) their Russian language skills, will gain new perspectives and experience at MGIMO, and will leave the program with broadened knowledge across a wide range of topics and contemporary issues.
The Program is suitable for individuals of all ages - our youngest graduate was 18 years old, while our oldest Alumni was 71. As far as geographic range is concerned, we've had the privilege of teaching students from 32 countries. The core requirements for our students are an excellent grasp of the English language, an insatiable appetite for knowledge and eagerness to learn!


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Our handy-dandy shipping calculator on the website is pretty smart! The most accurate way to get an estimate is to create a "mockup" order containing boxes in the same size, materials, and style as the boxes you plan to order (no need to worry about artwork for this!):
- Select the quantity, then click "Place Order."
- On the checkout page, enter your full shipping address and the calculator will work its magic. You'll see all of the options available to your address for this order, along with shipping costs and estimated transit times.
No need to worry, nothing will be charged on your card until you have submitted your order. These estimates are completely free. ;)
For large orders where LTL Freight may be used, contact our Customer Support team and we'll be happy to help with a freight estimation. We'll need to know your shipping address, as well as the dimensions, style, and quantity of your order, as well as how quickly you are looking to receive it.


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You already know it can save you cash with homemade Christmas gifts and Christmas appetizers you make your self, however your Christmas decorations do not have to break the financial institution either. Victoria Smith pens the weblog SF Lady By Bay and describes herself as a blogger, photographer, picture stylist, design junkie and bonafide flea market queen. It's her 'Vignettes' board, which features infinite ideas about the best way to style a vignette in a forgotten nook or overlooked area in your house. You needn't spend big to create a fantastic home – discover some attainable trendy home concepts from this gallery. The Lamps Plus dwelling decor catalog options lamps, light fixtures, ceiling followers, out of doors lights, and even some home decor reminiscent of mirrors and equipment. When the time comes to design your personal kitchen, you'll be spoiled for choice with everything from cabinetry choices and benchtops to flooring plan ideas and art decor. Unconventional trendy furniture pieces add more than character to a room, they elicit conversation. Good dwelling ideas: Planning know-how early helps to keep it as invisible as doable.
Take a look at the overall really feel of the room and chances are you'll get some concepts on some budget decorating you are able to do or even some inspiration to change across the decor you have already got. The Restoration Hardware house decor catalog features high quality furnishings, textiles, hardware, linens, rugs, drapery, wall decor, and accessories. Read extra: Turn those new home ideas into reality and discover gorgeous garden ideas , pool concepts and out of doors dwelling concepts to make your out of doors space shine.
Montreal-primarily based Gabrielle Savoie of Savvy House describes her blog as a web based decor destination influenced by a love for the coastal way of life, mixed with the tradition and luxury of American design." As such, her Pinterest boards characteristic a beautiful mix of unique and curated images exhibiting off among the finest coastal design – from the Hamptons to Palm Seashore.
Even in case you've bought a tiny square footage to work with (in that case – try our small lounge concepts ) or significantly love a particular fashion ( fashionable lounge ideas , anybody?) we have an enormous quantity of beautiful inspiration and knowledgeable advice to guide you through the lounge design course of.
By folding your Reader's Digest journal page over web page, you'll be able to flip it right into a cute, low-cost Christmas magazine craft. Let inspiration find you – keep the house décor concepts coming while going over our vastly diverse house décor assortment. Then check out our pick of the perfect living room wallpaper ideas From alcove shelving to fashionable media units, keep clutter at bay with some intelligent living room storage concepts and provides rugs, cushions and different decorative accessories space to shine.


text: Group-Lasso INTERaction-NET. Fits linear pairwise-interaction models that satisfy strong hierarchy: if an interaction coefficient is estimated to be nonzero, then its two associated main effects also have nonzero estimated coefficients. Accommodates categorical variables (factors) with arbitrary numbers of levels, continuous variables, and combinations thereof. Implements the machinery described in the paper "Learning interactions via hierarchical group-lasso regularization" (JCGS 2015, Volume 24, Issue 3). Michael Lim & Trevor Hastie (2015)


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MySmilePlan means you can have the dental treatment you need, when you need it with no interest – EVER.
The plan is available for all general, cosmetic, orthodontic and specialist dental care. The cost is broken down into easy, fortnightly credit card payments over either 9 or 15 months, depending on your plan value.
There are three different payment plans available; one for dental treatment up to the value of $500 with payments spread over 3 months, one for treatment up to the value of $2,000 with payments over 9 months and one for treatment up to the value of $9,000 spread over 15 months. General, cosmetic and specialist dental services are all covered and the easy application process is performed at the practice and takes just 2 minutes.
This plan was created to ensure patients can access the dentistry they need when they need it.
Find our more here.


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BP: Gulf of Mexico oil spill 'shared responsibility'
- Published
A senior BP executive has told a US court that the oil giant was not solely responsible for the 2010 Gulf of Mexico oil spill.
Rig operator Transocean and contractor Halliburton must also bear portions of the blame for the explosion and resulting spill, Lamar McKay said.
The trial, in New Orleans, will determine liability for the spill.
BP could face a huge fine, despite agreeing in 2012 to pay $4.5bn (£2.9bn) to settle criminal charges.
An unfavourable trial verdict could see the firm liable for the biggest civil fine in history, of up to $17.6bn.
It has also paid $7.8bn in a settlement with people and businesses affected.
'Team effort'
In the first testimony from a high-ranking BP executive, Mr McKay stressed that all those involved in the Deepwater Horizon disaster should take some of the blame.
While BP was extracting the oil from underneath the Gulf of Mexico when the Macondo well exploded, the Deepwater Horizon rig itself was owned by Transocean. Cement used to seal the well was provided by Halliburton.
The Macondo explosion killed 11 men and released an estimated four million barrels of oil into the Gulf over 84 days. Since the leak was plugged, the debate over who was responsible has raged on.
"I think that's a shared responsibility, to manage the safety and the risk,'' Mr McKay told the court.
"Sometimes contractors manage that risk. Sometimes we do. Most of the time it's a team effort.''
He said the explosion was a "tragic accident" resulting from a "risk that was identified".
An expert witness testifying on behalf of the plaintiffs - the US Department of Justice and the US states affected by the spill - countered that the disaster was a direct result of poor management and heavy cost-cutting at BP.
"It's a classic failure of management and leadership in BP," said Robert Bea, an engineering professor at the University of California, Berkeley, who previously worked as a safety expert for BP.
He described BP as "too lean" following rounds of cost-cutting that led up to the disaster.
On the first day of the trial, a lawyer for the justice department said the disaster resulted from BP's "culture of corporate recklessness".
"Despite BP's attempts to shift the blame to other parties," Mike Underhill said, "by far the primary fault for this disaster belongs to BP".
One of the lawyers for the plaintiffs, Jim Roy, said BP had put "production over protection, profits over safety".
Mr Roy also attacked the rig's operator, Transocean, saying the company's safety official on the rig had received little training: "His training consisted of a three-day course. Amazingly, he had never been aboard the Deepwater Horizon."
He did not spare contractor Halliburton, either, saying it deserved some of the blame for providing BP with cementing of the Macondo well that was "poorly designed, not properly tested and was unstable".
Fading giant?
The trial will determine the causes of the spill, and assign responsibility to the parties involved, including BP, Halliburton, Transocean, and Cameron, which manufactured the blowout preventer meant to stop oil leaks.
Later, it will determine how much oil actually leaked, which will lead to the calculation of how much the oil companies owe in civil fines.
It is expected to be one of the biggest and costliest trials in decades.
BP chief executive Bob Dudley has said he firmly believes the company was not grossly negligent.
The trial could last for months, but the risks are so great for BP that it may try to reach a settlement, analysts suggest.
Robert Percival, an environmental law professor at the University of Maryland, said: "The risk for both sides is so great - for BP it's their name, reputation and future contracts with the US government. For the US government it's all the resources they're spending on the trial - particularly if BP is not found grossly negligent."
The second part of the trial, set to begin in early autumn, will attempt to determine how much oil was leaked, which would then determine the size of the federal fine.
The Department of Justice intends to demonstrate BP was grossly negligent, which puts the maximum penalty at about $17.6bn.
However, on top of that, the Gulf states of Louisiana, Alabama, Mississippi and Florida are demanding an additional $34bn in damages under the Oil Pollution Act, citing uncertainty over the long-term effects of the spill on their coastline as well as economic losses and property damage.
But BP has said it will "defend vigorously" against the claims, saying the methodologies used to calculate them were "seriously flawed".
Once the world's second-biggest oil company, BP has fallen to fourth place among the "oil majors" after selling off billions of dollars worth of assets to set aside money to cover liabilities related to the disaster.


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|(no description provided)
|Studies of proton pumping mechanism in BA3 type cytochrome oxidase of thermus thermophilus and CBB3 type cytochrome oxidase of vibrio cholerae
|Chang, Hsin-Yang
|Director of Research:
|Gennis, Robert B.
|Doctoral Committee Chair(s):
|Gennis, Robert B.
|Doctoral Committee Member(s):
|Martinis, Susan A.; Huang, Raven H.; Tajkhorshid, Emad
|Department / Program:
|Degree Granting Institution:
|University of Illinois at Urbana-Champaign
|ba3 cytochrome c oxidase
cbb3 cytochrome c oxidase
|Oxygen reductase members of the heme-copper oxidoreductase superfamily are redox-driven proton pumps which couple the free energy liberated from the reduction of O2 to the translocation of protons across the membrane. The A-family requires two proton input channels (D- and K-channels) to transfer protons used for oxygen reduction chemistry and proton pumping, with the D-channel transporting all pumped protons. We use comparative genomics, site-directed mutagenesis, and redox kinetics to demonstrate that the B-family of oxygen reductases utilize only one proton input channel, structurally analogous to the K-channel, for the delivery of protons for both chemistry and pumping. Since all of the oxygen reductase families are evolutionary related and perform the same chemistry for oxygen reduction, we propose that they must also share an evolutionary conserved mechanism for proton pumping. Comparative analysis between all oxygen reductase families identifies the A-propionate of the active-site heme as the most likely proton loading and the kinetic gating site. According to the crystal structure of ba3 oxidase in T. thermophilus, two highly conserved residues, D372 and H376 which are located at the interface of subunit I and II of complex, form ion pairs with the A-propionate of active-site heme a3. A set of mutations of D372 and H376 are made and examined in this work. Interestingly, mutations of D372I and D372A still remain ~50% and 15% electron transfer activity respectively, but abolished proton translocation. Furthermore, the reduced minus oxidized FTIR difference spectra of the D372I also show significant changes in the protonated heme propionate signals. These results suggest that the H-bonding network A-propionate heme a3-D372 might play an important role in proton translocation. Heme c binding proteins have been well identified in Vibrio cholerae. Of these, two predicted c-type cytochromes, diheme c4 (encoded from gene cycA) and monoheme c5 (encodes from gene cycB), have high conservative amino acid residues in all of the Vibrio strains and are significant homologues in other known bacteria. Both of them are predicted to transfer electrons on the respiratory chain in V. cholerae. In the current work, cytochromes c4 and c5 have been cloned and expressed heterologously in E. coli. It is shown that reduced cytochrome c4 is a substrate for the purified cbb3 oxygen reductase and can support steady state oxygen reductase activity of at least 300 e-1/s. In contrast, reduced cytochrome c5 is not a substrate for the cbb3 oxygen reductase. Cyclic voltammetry was used to determine that the midpoint potentials of the two hemes in cytochrome c4 are 240 mV and 340 mV (vs SHE), similar to the electrochemical properties of other c4-type cytochromes. The majority of cytochrome c4's are found in β- and γ- proteobacteria. Genomic analysis shows a strong correlation between the presence of a c4-type cytochrome and a cbb3 oxygen reductase within the β- and γ- proteobacterial clades, supporting our proposal that cytochrome c4 is the likely natural electron donor to the cbb3 oxygen reductases within these organisms. These would include the β- proteobacteria Neisseria meningitidis and Neisseria gonnorhoeae, in which the cbb3 oxygen reductases are the only terminal oxidases in their respiratory chains, and the γ- proteobacterium Pseudomonas stutzeri.
|Issue Date:
|Rights Information:
|Copyright 2010 Hsin-Yang Chang
|Date Available in IDEALS:
|Date Deposited:
|May 2010
This item appears in the following Collection(s)
Graduate Dissertations and Theses at Illinois
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Biochemistry