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RNA extraction for microarray
Posted 13 May 2009 - 12:08 AM
I am wondering if RNA extraction by trizol is compatible with the microarray analysis (trizol extraction method with no further purification)?
Thanks in advance
have a nice day
Posted 13 May 2009 - 01:00 AM
column isolation of rna via columns (RNeasy or something similar) usually gets rid of DNA contaminations as well to a very high extend if you do not overload columns.
However, I know from colleagues who just did a trizol extraction without further purification afterwards.
Posted 13 May 2009 - 08:00 AM
ya actuelly I am wondering if I can do just like these people as I don't have much of RNA and using the kit I will lose a part of RNA
thanks again
Posted 14 May 2009 - 04:24 AM
How much RNA do you have?
Posted 01 July 2009 - 05:31 AM
using only Trizol extraction means there are still phenol / salts present in your sample.
This means that or the labeling of your RNA will not be optimal OR/AND the stringency of your hybridization conditions will drop because the phenol is saturated with salt.
So in my opinon you should perform a precipitation but better would be a silica column purification after the phenol/chloroform extraction.
Posted 05 August 2009 - 05:19 AM
Really need your advise. Thanks.
Posted 05 November 2009 - 02:18 PM
what type of microarray do you use?
Posted 08 November 2009 - 03:38 AM
One thing you should think about when using columns for purification ...you might lose all transcripts that are smaller than for example 200 bp, because they won't bound to the matrix of the column ...so this information will be lost! Therefore we prefere using classical methods.
Hope this will help you somehow!
Posted 08 November 2009 - 09:08 PM
Posted 17 November 2009 - 06:20 AM
We do first Trizol extraction, than get rid of the DNA by DNase I digestion followed by Phenol:Chloroform:Isoamylalcohol extraction ...than the DNA should be pure enough to use it for genertion of cDNA by RT. This works great for bacterial RNA because we have loads of them and don't have to worry that we lose some amount of RNA during the two extraction steps.
One thing you should think about when using columns for purification ...you might lose all transcripts that are smaller than for example 200 bp, because they won't bound to the matrix of the column ...so this information will be lost! Therefore we prefere using classical methods.
Hope this will help you somehow!
And is trizol extraction also good when you will do the DNA-ase treatment of DNA, using filter units, like millipore? and for the application of qPCR?
with kind regards,
Posted 17 November 2009 - 07:11 AM
I suppose it was DNase treatment for RNA..but I didnt get which method of DNase treatment u were refering to here! You can do a trizol extraction, then do a DNAse digestion on column or use the Ambion Turbo DNase to avoid a column cleanup step where you lose the small RNA fraction
Posted 17 November 2009 - 08:07 AM
Posted 17 November 2009 - 08:08 PM
I found this a very good/ clean protocol. I used to use this to extract human cell line RNA for microarray.
Posted 18 November 2009 - 07:05 AM
I had been using normal Trizol extracted RNA without a DNase cleanup for microarrays for a long time without any problem. I would choose Trizol over a column because its quite inexpensive compared to column, quite robust and has a very vast range of starting amounts of cells/tissue which can be used (100s to >10^7 per ml of Trizol) and the best part, it works for all the kinds of stuff, be it cell lines, human tissues, plants or yeast!!!
Edited by gogreen, 18 November 2009 - 07:09 AM.
Posted 18 November 2009 - 05:27 PM
@ sanjiun - The protocol uses Trizol and Qiagen columns for the extraction!! If you belong to a lab which is not "quite well funded", this might be expensive for a large number of samples.
I use that cause my samples are not too many.
Since microarray itself is already an expensive experiment. LOL
So my supervisor wanna make sure everything worked well and get good RNA for microarray.


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Mayor's Office
Mayor Chris Beutler and City Council Member Adam Hornung today announced that they are asking the City Council to approve a resolution directing the City Audit Advisory Board to examine StarTran's service delivery model and efficiency. The goal, they said, is to look at saving tax dollars while providing a stronger public transportation system.
The resolution calls for an operational review to focus on the following areas:
"Every dollar we save as a result of increased efficiency is a dollar that can be spent on public safety, parks and libraries, the services that make Lincoln a great community," Mayor Beutler said. "The proposed StarTran resolution can help us discover those cost cutting possibilities. I am also intrigued by the potential the resolution offers for a greener community. Many public transportation innovations revolve around energy savings. Star Tran consumes over 340,000 gallons of fuel each year. A more efficient Star Tran could mean a more sustainable community."
"An examination of StarTran would give us the information we need to determine if we can implement new innovations that have been successful elsewhere," Hornung said. "I am confident that an Audit board led study of the issue can provide the direction and guidance for a public transportation system as good as the people who use it and as frugal as the taxpayers who support it."
The City Council approved the Mayor's proposal to create a City Audit Advisory Board in 2007. The Board is empowered to contract for internal, financial and operational audits and performance reviews of City government. The six-member Board includes three Mayoral appointees and three appointed by the Council.
The City Council recommends projects, and the Audit Advisory Board determines the scope of the review. The Board previously examined City fund balances in the spring of 2009. The City has about $251,000 from previous budget years in an account to provide for these activities.
The resolution is on the City Council agenda for its meeting which begins at 5:30 p.m. Monday, January 31.for final report. If the resolution is approved, the audit board will further define the scope of the study and issue an RFP for a contractor to complete the performance review.
City Audit Advisory Board:

subset: commoncrawl


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There are two people in our family who are just so funny that you love to be around them and often the comment is heard, "if we could get Sharon and Carol together one day, we would die from laughter."
Sharon lives in Indiana and Carol in Wisconsin, so it has not happened yet, but Sharon has begun blogging. She was shamed into it really by a couple of bloggers, okay, me and her friend. She tried her hand, Just who are you people? « Braindebris's Weblog, and was (as we suspected) hysterical. She is one of 'those' people, who if she wrote regularly enough would be publishing a book. She is that funny, just check out the The Immortal Rabbit « Braindebris's Weblog if you want to split a gut.
She took a long break from blogging, much to our disgust, and is back now on a weekly blog schedule. You would think she has a life or something! She also mentioned it takes a good hour to put a blog post together. Who knew!
So, this morning, I checked how her weekend was and she brought up the often mysterious workings of the blogosphere and the people who take the time to read your blog but rarely write comments.
My readership is not huge, it has dropped since I have gotten less regular in posting. However, in the beginning, when my readership would hit 1,000 for the month, I never had commenters, other than one. There were 999 people out there lurking. XUP always comments, all the way from Canada. Aren't you ashamed fellow Americans? I have to get comments from Canada. Pollyanna Rainbow Sunshine and the Needles of Doom often commented, but I have been really remiss commenting on their blog lately. Don't you love that name though!
I have also been blogging a bit less lately myself, as I work on my eternal update to my website. I feel like one of those people on the commercial, staring wide eyes, pupils dilated from staring at the screen, mind numb … anyway, you get the not pretty picture. I only have one section left to go on the website and am soooo look forward to it being done.
This morning, here is a copy my monthly stats. Notice the slow decline.
So, I checked Search terms.
"no blood for oil Viggo" – as in Viggo Mortensen, no surprise there. Half of my readership is searching for "Viggo" – Talk about stalkers!
"Worms …" – EUWWW! especially when in the house.
"actor with low ears" – Okay, is this person casting a movie? I have written about actors, and I have written a post due to the lovely commercial based on the old song "do your ears hang low. " But, I have NEVER written about an actor with low ears. I am not even sure why you would want to find an actor with low ears.
In an effort to raise my stats, I realize that I have three sure winners:
- Viggo Mortensen
- photography
- Movie reviews
Okay, Viggo Mortensen is hot and I do understand the fascination. He keeps his private life mysterious and he's hot and he likes dark chocolate and he's hot. What more could you want? The only thing is, I do not actually know Mr. Mortensen (I love his name by the way— Viggo Mortensen—-it's lyrical. This is coming from someone named VanVleck. Not so lyrical.
So, I shall now vow to make more of an effort to review movies about Viggo Mortensen and you, my kind readers (numbers 13 to 30 and occasionally 1,000) shall occasionally drop me a line to let me know what you like, or do not like, on my blog.
Oh, and I will try not to be a stalker myself.


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Recipe from Karina of TX
2 cups of boneless pork chops
1/2 cup of stewed tomatoes
1/2 can of tomato sauce
2 Mexican squash or 1 Cabbage
1/2 cup of white onion
1 tsp garlic powder
1 tsp cumin powder
1 tsp black pepper
salt (to taste)
Cut the pork chops into 1/2 to 1-inch pieces. Then brown the pork chops. Add in a little water to keep the meat moist. Then add in the white onion. When the pork chops are fully cooked, add in the stewed tomatos, the tomato sauce, and the seasonings. Bring the mixture to a boil then cover it and allow it to simmer for 15 minutes. Cut a squash into thin round slices or shred a cabbage. Then add the shreds or slices to the pork chops and allow the mixture to simmer for another 15 to 20 minutes. Be sure to keep the mixture covered while simmering so that the veggies and pork will soften as they cook. If needed add 1/2 cup of water so that veggies will be at least half way submerged in the sauce to absorb flavor. Serve with white or Spanish rice. Enjoy!
*DISCLAIMER*: The information contained in or provided through this site section is intended for general consumer understanding and education only and is not intended to be and is not a substitute for professional advice. Use of this site section and any information contained on or provided through this site section is at your own risk and any information contained on or provided through this site section is provided on an "as is" basis without any representations or warranties.


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Reality Check: Super PAC ad attacking Obama uses selective editing
Restore Our Future is running TV ad
MADISON, Wis. — Most of the television ads for the presidential race have been from committees weighing in on behalf of candidates rather than the candidates themselves.
In one of the opening salvos of the presidential campaign in Wisconsin, a political action committee supporting Republican Mitt Romney uses selective editing to distort President Barack Obama's comments.
The group running the ad, Restore Our Future, is a super PAC. It can raise and spend unlimited amounts of money and is run by former aides to Romney.
The investment and banking industry are the group's biggest donors.
"If you had President Obama's record, what would you do? Would you joke?" the ad's narrator says. The ad then shows a clip of Obama saying, "Shovel ready was not as … shovel ready as we expected."
WISC-TV found this needs clarification. This was a joke made during a June 2011 meeting of the president's jobs council. The comment was after an official from the railroad industry was explaining some problems with the permitting process for projects that may have been done by stimulus work.16162958
The questioner said: "We see firsthand what happens when you try to permit a project in this country, and it can delay things from months to years and, in many cases, it can cause the project to be abandoned. I'm sure that when you implemented the recovery act, your staff briefed you on many of the challenges and the impact on putting Americans back to work."
Obama replied, "And that's exactly what we see in American business as well. Shovel ready wasn't as shovel ready as we expected."
The ad's narrator then asks whether a person with Obama's record would "deny reality?" The ad then shows a clip of Obama saying, "We tried our plan and it worked."
WISC-TV found this to be misleading. Obama was not referring to an economic plan he'd tried during his term. Instead, he was promoting a plan he wanted to use, saying it had worked during the Clinton administration.
Here is the full text of what Obama said in Oakland:
"I'm also going to ask anybody making over $250,000 a year to go back to the tax rates they were paying under Bill Clinton, back when our economy created 23 million new jobs. The biggest budget surplus in history and everybody did well. Just like we've tried their (the Republicans') plan, we tried our plan — and it worked. That's the difference," Obama said.
And then there's the last Obama quote in the ad, a quote that has gotten a lot of play. The ad shows a clip of Obama saying, "The private sector is doing fine."
WISC-TV found this needs clarification. Obama said this after saying how many jobs he believed had been created and making a comparison.
"We've created 4.3 million jobs over the last 27 months, over 800,000 just this year alone. The private sector is doing fine. Where we're seeing weaknesses in our economy have to do with state and local government," Obama said.
The ad ends by attacking the president for "shameful and dishonest attacks," but a WISC-TV analysis found that this ad isn't honest either.
Leading up to the election, WISC-TV will be putting the claims candidates are making and the ads backing them through a "Reality Check." Send your ideas for a Reality Check to firstname.lastname@example.com.


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Winter Weather in the Middle of Spring
I don't know about you but when I think about the month of May, I'm thinking about shorts, t-shift, and walks in the park with the birds singing. Contrary to that, tomorrow morning I'll be hurrying around looking for the sweaters I have already packed away for the season. I won't be walking - I'll be staying inside.... and the birds won't be singing - they'll be shivering.
Winter weather may be making an unwelcome comeback on Friday morning. It looks like just before midnight tonight parts of NE Oklahoma and NW Arkansas will begin to watch rain showers change over to a wintry mix and eventually snow.
That chance of the snow or rain snow mix will be with us throughout much of your early Friday forecast. In fact, there may even be parts of the River Valley that need to be concerned about Friday morning show as well.
This is a very fluid situation so you'll want to stay with us for the latest developments as the rain and colder temperatures settle in.
Laura Huckabee / meteorologist


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25 December 2012 | Opinion | By BioSpectrum Bureau
Ms Sarah Rickwood, director, thought leadership, IMS Health, UK
The changing outlook for biosimilars comes at a time when the global pharmaceutical market is feeling the combined impact of two key events: a period of unprecedented patent expirations on many of the world's largest pharmaceutical brands, and a financial crisis that has required healthcare systems to make significant and sustained cost reductions. For payers in the advanced markets, limited economic growth and pressures on healthcare make the patent cliff a true generic dividend, enabling much needed savings to be realized.
However, as potential savings from generics start to decline in these countries over the next five-to-ten years, while the imperative to reduce expenditure continues to grow, payers face an urgent need to find new ways of rationalizing resources. Biologics - among the most expensive pharmacotherapies available and now approaching their own swathe of patent expirations - potentially represent the most lucrative source of savings on drug expenditure for Western nations after 2015.
Biosimilars may be the key that helps them to realize this opportunity. Biosimilars also bring clear potential for payers in the emerging pharmaceutical or "pharmerging" markets, such as Brazil, India and China. Here, the need to broaden healthcare coverage to large populations increasingly must be balanced against limited budgets and growing demand for innovative drugs. Biosimilars offer one way of widening access and enabling better value to be obtained from the money spent on healthcare. In some cases (such as South Korea, India and Brazil) they are seen as a key macroeconomic driver of growth, attracting foreign capital by creating manufacturing and R&D centers of excellence.
The imperative to find cost-effective alternatives to biologics reflects the growing demand for these specialty drugs. Since their origins in the 1980s, biologics have prospered into a $138 billion market (2010), fuelled by such key launches as recombinant insulins, human growth hormone, alteplase, erythropoietins, granulocyte colony stimulating factors and then monoclonal antibodies, among others. Currently accounting for 16 percent of global pharmaceutical expenditure and significantly outpacing total branded sales, biologics will continue to out-perform the global market as more innovative products deliver new treatment options for a growing range of indications.
A number of top-selling biologic brands, including Herceptin, Enbrel, Humalog, MabThera, Remicade and Aranesp, are due to lose product patent protection over the next five years, opening up a wealth of new possibilities for biosimilars players. Key therapy areas such as cancer, diabetes and rheumatoid arthritis will spearhead this new wave of biosimilars, with attention focused on the real prizes of anti-TNF MAbs, MAbs for oncology, and insulins.


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Harbron: A colorful chemistry for undergraduate researchers
Note: This article originally appeared in the W&M Alumni Magazine. —Ed.
Elizabeth Harbron has an automatic advantage over her colleagues in the chemistry department when it comes to recruiting students into their research labs: she works with chemicals that change color, light up and glow.
"The inherent appeal of light and color can't be understated. So much of organic chemistry is taking white powder A and reacting it with clear reagent B and producing white powder C," says Harbron, associate professor of chemistry. "But everything in my lab either is very colorful or changes color in response to light or some other stimulus. There's a 'cool factor' to it."
Sometimes the Harbron lab incorporates lasers in its work, which gives a nice boost to the light and color and doesn't hurt the cool factor one bit. Harbron and her student colleagues are aiming to put the hue and glow to work by figuring out the basic chemistry that could lead to a number of practical devices, from sensors to laptop screens readable in full sunlight.
Harbron is just one of the more colorful examples of William and Mary faculty who blend teaching and research. In February, she was awarded the College's Thomas Jefferson Teaching Award, an annual honor that is especially significant when you consider that she teaches Organic Chemistry I and II — watershed courses that have enrollments approaching (and occasionally topping) 200 students. The previous month, she became one of just six U.S. chemists to be named a Henry Dreyfus Teacher-Scholar. The award recognizes chemistry faculty who not only are accomplished researchers themselves, but who also incorporate undergraduate students into their research. Among her other honors, Harbron is a 2008 recipient of the Alumni Fellowship Award.
Peer Mentoring
Harbron has settled on eight as the optimal number of students in a lab group. It's important to have a group that works well together and over the years, her labs have become predominantly female. Usually there is one guy in the lab each year, and Harbron says that it's "like having a brother in a roomful of sisters."
"Initially I was striving for 50/50 gender representation in my group, just because I thought that gave us the best balance of characteristics," she says. "Becoming predominantly female happened a little bit by accident just in terms of the students I happened to get one year. It has sort of started to sustain itself and I've started to think about that a lot more critically."
She points out that we still have fewer women going into science than men and says the gender disparity in America's labs is exacerbated by the "leaky pipeline" phenomenon, which sees women dropping out of science at various points along the way at a higher rate.
"I've realized that the model of peer mentoring I had already started in my lab worked particularly well with women," Harbron says. "So, I think my approach addresses some of the concerns that may be more heavily felt in a female population."
One such concern, she says, is confidence — not self-confidence, but scientific confidence. "By that, I mean the confidence to use your hands in the lab, to ask questions about the science, to teach the science to other people, all those sorts of things," she says. "When someone who formerly has been a straight-A student moves from the classroom to the lab, there's usually a point in which they think, 'I don't understand what to do now' or 'I don't understand how to work this equipment.' I think girls are so afraid of being wrong — or of making mistakes — that they especially have trouble with that transition."
Peer mentoring in the Harbron lab is made possible by including students from every level. Harbron works with all the students several times a week, but she encourages her upperclass undergraduates and her master's students to mentor the newbies. The arrangement sharpens the experienced lab members, as Harbron points out: "You really figure out quickly what you don't know when you're trying to explain it to someone." The freshmen and sophomores get a chance to put what they might be afraid are dumb questions to a fellow student.
"Sometimes the professor can be a little scary, a little intimidating," Harbron notes. "I do want to be there in the lab, showing them, guiding them, helping them interpret their results. But, if I stand there too much, I think that it also takes away from their developing independence."
Passing the Torch
The Harbron lab style of peer mentoring also allows for continuity of individual research projects. Take, for instance, what Harbron calls the Pink Projects — a pair of investigations into the potential of chemical- and light-activated dyes as sensors. One of the pink projects, involving a dye known as rhodamine hydroxy-benzaldehyde, was begun by Harbron working with Becca Allred '10. During her junior year, Courtney Roberts '11 was one of the chemistry students in a class called Introduction to Chemical Research, in which all the chem faculty give brief talks on their research.
"I'm really interested in organic chemistry," Roberts says. "The organic part interested me. Then Professor Harbron did a little demonstration with the fluorescence and that grabbed my attention."
Roberts joined Allred's project, taking the lead after Allred graduated. As her own graduation date approached, she recruited her rugby teammate, Grace Purnell '14, to the lab.
"Because I know Courtney and I like working with her, Professor Harbron thought it would be a good idea for us to start collaborating on her projects so that when she graduates, I can become the person in charge of doing that research," Purnell says?.
Purnell says that she works about three hours a week in the lab, about half the time that Roberts works. The scope of the rhodamine hydroxybenzaldehyde project (like most lab inquiries) extends beyond the time any single undergraduate can devote to it, but the passing of the torch from Allred to Roberts to Purnell allows the Harbron lab to keep the research project moving.
Harbron says that peer mentoring and the collaborative culture evident throughout many of William and Mary's science labs will serve the College's science graduates well after they leave Williamsburg.
"Science is much more team-based now than it was in the past and I think that's only going to continue," she says. "I think it's pretty rare for someone to be doing all of their work in isolation. It's very common now in research universities and graduate programs to have a lot of collaborations between different labs. That means that students need to be able to work with other people and explain what they're doing with these other people and figure out the interface."
And for William and Mary students like Courtney Roberts — currently scouting out graduate programs across the country — that means they're already one step ahead of the game.


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(Boston) An international group of researchers has discovered seven new regions of the human genomecalled locithat are associated with increased risk of age-related macular degeneration (AMD), a leading cause of blindness. The AMD Gene Consortium, a network of international investigators representing 18 research groups, also confirmed 12 loci identified in previous studies. The study, which is published online in Nature Genetics and represents the most comprehensive genome-wide analysis of genetic variations associated with AMD, was supported by the National Eye Institute (NEI), a part of the National Institutes of Health.
Lindsay A. Farrer, PhD, chief of the biomedical genetics section and professor at Boston University Schools of Medicine (BUSM) and Public Health (BUSPH), is co-lead author of the study.
"This compelling analysis by the AMD Gene Consortium demonstrates the enormous value of effective collaboration," said NEI Director Paul A. Sieving, MD, PhD. "Combining data from multiple studies, this international effort provides insight into the molecular basis of AMD, which will help researchers search for causes of the disease and will inform future development of new diagnostic and treatment strategies."
Since the 2005 discovery that certain variations in the gene for complement factor Ha component of the immune systemare associated with major risk for AMD, research groups around the world have conducted genome-wide association studies to identify other loci that affect AMD risk. These studies were made possible by tools developed through the Human Genome Project, which mapped human genes, and related projects, such the International HapMap Project, which identified common patterns of genetic variation within the human genome.
The AMD Gene Consortium combined data from 18 research groups to increase the power of prior analyses. The current analysis identified seven new loci near genes. As with the previously discovered 12 loci, these seven loci are scattered throughout the genome on many different chromosomes.
"A large number of samples was needed to detect additional genetic variants that have small but significant influences on a person's disease risk," said Hemin Chin, PhD, NEI associate director for ophthalmic genetics, who assembled the consortium and helped coordinate the study. "By cataloging genetic variations associated with AMD, scientists are better equipped to target corresponding biological pathways and study how they might interact and change with age or other factors, such as smoking."
The consortium's analysis included data from more than 17,100 people with the most advanced and severe forms of AMD, which were compared to data from more than 60,000 people without AMD. The 19 loci that were found to be associated with AMD implicate a variety of biological functions, including regulation of the immune system, maintenance of cellular structure, growth and permeability of blood vessels, lipid metabolism and atherosclerosis.
As with other common diseases, such as type 2 diabetes, an individual person's risk for getting AMD is likely determined not by one but many genes. Further comprehensive DNA analysis of the areas around the 19 loci identified by the AMD Gene Consortium could turn up undiscovered rare genetic variants with a disproportionately large effect on AMD risk. Discovery of such genes could greatly advance scientists' understanding of AMD pathogenesis and their quest for more effective treatments.
AMD affects the macula, a region of the retina responsible for central vision. The retina is the layer of light-sensitive tissue in the back of the eye that houses rod and cone photoreceptor cells. Compared with the rest of the retina, the macula is especially dense with cone photoreceptors and is what humans rely on for tasks that require sharp vision, such as reading, driving and recognizing faces. As AMD progresses, such tasks become more difficult and eventually impossible. Some kinds of AMD are treatable if detected early, but no cure exists. An estimated 2 million Americans have AMD.
Scientists have shown that age, diet, and smoking influence a person's risk of developing AMD. Genetics also plays a strong role. AMD often runs in families and is more common among certain ethnicities, such as Asians and people of European descent. AMD typically presents later in life, but identifying genetic variants associated with the disease, all of which are present at birth, could help future studies determine how to stop the disease from progressing and even from occurring.
"Genetic research allows us to piece together disease pathways that may have their starting point much earlier in life," said Farrer. "These newly identified genes, individually and collectively, provide novel clues and targets to evaluate for their potential therapeutic benefits."
|Contact: Jenny Eriksen
Boston University Medical Center


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This site discusses phimosis in its specific forms of phimotic ring, frenulum breve, adhesions or skinbridges. During erection these conditions inhibit the relationship between foreskin and glans. This functionally
restricts the erection, and thus has an effect on the sexuality. With
our culture's attitudes on health care, it would be appropriate to monitor
boys before puberty and encourage early prevention.
2012 : note from author: My previous idea of monitoring boys before puberty is impractical, unecessary and now only of historical interest. please see Postscript.


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Sugar Rush: Doughnuts at Abbe's Donuts in Port Charlotte, FL
As much as I love a complicated, multi-component, modernist dessert, it's really tough to beat a fresh, old-school doughnut, and that's what you'll find at Abbe's Donuts. The North Port location of the family-run pair of shops has been serving up sugary deep-fried dough and friendly conversation for over three decades, while the second location, in the Port Charlotte Square shopping center, recently celebrated its 1st anniversary.
Inside, they've got all of the standards: jelly-filled powdered doughnuts, glazed cake varieties, and raised doughnuts with maple and chocolate icing. But once I locked eyes on the apple fritter ($1.25), ordering one was a foregone conclusion. It dwarfed everything else in the case, and practically glistened with glaze.
The fritter (which was still warm) contained big chunks of apple, and, most importantly, had the ideal texture: soft on the outside, with a crunchy crust. You could pull off sugar coated chunks of dough, in search of the soft, baked apple bits, or just attack it in big shark-sized bites and hope to land on an apple nugget.
Another worthy option is the peanut butter cream (90 cents), a yeast doughnut that is fashioned like a whoopie pie, with a sweet peanut butter spread between the two doughnut halves and some chocolate glaze on top.
It's a bit like eating a super-sweet peanut butter sandwich, so much so that I was craving a little bit of grape jelly to squeeze on top.
The shop also whips up a special "doughnut of the day". In the past, flavors have included pumpkin eggnog, carrot cake, cinnamon french toast, and cranberry orange. With so many doughnuts to choose from, I'd go in prepared to try at least three different flavors—and don't be surprised if you can't get out of the store without an even dozen.
Abbe's Donuts
2150 Tamiami Trail, Port Charlotte, FL 33948 (map) 941-979-5086; abbesdonutshop.com
All products linked here have been independently selected by our editors. We may earn a commission on purchases, as described in our affiliate policy.


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The Folsom Lake Symphony
The Folsom Lake Symphony was established in 2004 to bring beautiful symphonic music to the Folsom Lake region. Through the commitment of our Board of Directors, Maestro Peter Jaffe, our dedicated musicians and scores of hard-working volunteers, we are proud to be the premier orchestra for Folsom and all of the surrounding communities.
Our mission includes two important goals: present high-quality symphonic performances for our audiences and educate young people to enjoy and appreciate classical music. Maestro Jaffe continues to lead exciting symphonic performances while bringing some new musical experiences to our patrons.


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A collection of scholarly works about individual liberty and free markets. A project of Liberty Fund, Inc.
1711 - 1776
Historical Period:
The 18th Century
David Hume (1711-1776) was a moral philosopher and historian and a leading member of the Scottish Enlightenment. In philosophy he was a skeptic. In his multi-volume History of England he showed how the rule of law and the creation of an independent judiciary created the foundation for liberty in England. Hume also wrote on economics, was a personal friend of Adam Smith,and was a proponent of free trade. His works highlighted the neutrality of money and the errors of the mercantilists (whose flawed theories in favor of increased exports in order to build up a stock of gold remain the foundations of many public policies even today).


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Avakin Life Hack Generator is a great new tool for Avakin game. . Today we really want to present this tool and all it's features. Our team has conducted a study among the players of the famous Avakin game. The results of this study show that many players are unhappy because they can not tighten enough […]
Posts Tagged 'avakin life hack generator no survey'
Avakin Life Hack Generator
Posted by James Carter on May 23rd, 2019


text:
Dale Ray Phillips
Assistant Professor
"Phillips's prose flashes powerful unpredictability with every delicious little shock." – Publishers Weekly
"Take care of the sentence, and the sentence will take care of you." – Dale Ray Phillips
M.F.A., Creative Writing
University of Arkansas-Fayetteville
M.A., English
Hollins College
B.A., English
UNC Chapel Hill
B.A., Anthropology
UNC Chapel Hill
My People's Waltz. W.W. Norton and Company, 1999. Paperback edition by HarperCollins Perennial, 2000.
Highlighted Publications
My People's Waltz. W.W. Norton and Company, 1999. Paperback edition by HarperCollins Perennial, 2000.
Selected Publications
"Having at It." The Oxford American Best of the South, 2012.
"Surviving the Uncles and Learning How to Write—Arkansas Style." The Oxford American Education Issue, 2011.
"Hillbilly Fishing." The Oxford American Best of the South, 2010.
"My Father's Ark." New Madrid, Winter 2007.
"Haw River Cosmology." Zoetrope: All-Story 4:2, Summer 2000.
"What It Cost Travelers." The Greensboro Review, February 1999.
"The Woods At The Back Of Our Houses." Harpers, July 1998.
"Why I'm Talking." The Atlantic, March 1996.
"Corporal Love." Story, Spring 1996. Reprinted in New Stories from the South 1997: The Year's Best, Algonquin Books.
"Everything Quiet Like Church." Story, Spring 1994. Reprinted in New Stories from the South 1995: The Year's Best, Algonquin Books.
"What Men Love For." The Atlantic, April 1988. Reprinted in Best American Short Stories of 1989, Houghton Mifflin.
Courses Taught
American Literature
Creative Writing: Fiction and Poetry
Developmental English
English as a Second Language
Graduate Fiction Workshop
Introduction to Literature
Twenty-plus residencies in the Arkansas Schools World Literature
Areas of Specialization
Creative Writing
Rhetoric and Composition
Awards and Distinctions
Watkins Endowed Chair of Creative Writing, Murray State University, 2007-10
Judge, The Oxford American Southern Literature Poll, 2009
Pulitzer Prize nominee for My People's Waltz, 2000
Discover Great New Writers Award for My People's Waltz, 1999
The New York Times Editor's Choice Pick for My People's Waltz, 1999
Stories in New Stories From The South: The Year's Best, 1995 and 1997
North Carolina Distinguished Scholar Appointment, 1991
Story in Best American Short Stories, 1989
Esquire Pyramid of Literature, Summer Fiction Issue, July 1989
Three stories listed in Distinguished Stories section of Best American series
Honorable Mention, O. Henry series
Lily Peters Fellowship in Fiction, University of Arkansas, 1986-87
James Andrew Purdy Award for Fiction, Hollins College, 1985-85
Randall Jarrell Fellowship, University of North Carolina at Greensboro, 1978-79
7C-13 Faculty Hall
Murray State University
Murray KY 42071-3341


text:
Daily Trojan, Vol. 22, No. 102, March 09, 1931
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Irooeo staff (, M.0 ir, ,.keJ '0 se* J# y tod«y •" th* *"nu SOUTHERN A L 1 F O R N I A DAILY 1' TROJAN /OL- Los Angeles. California, Monday, March 9, 1931. IRMESS ARRANGED FOR AID OF JOBLESS jhestra is I0SENFOR IllSIC SHOW Hy Tompkins Will ct B 0 b Brown's danders. I " lh» ipcond successive year, f Tompkins will direct Bob orcbeitra when "Shlp-tbe annual extrava-1 presented in Bovard audl-17 and lg. Jlni *HI be remembered I wcoesiful wielding of the rt last year s musical com-»bu bid quite a little bit »no« in musical work, I been conductor of the Jimi bund while he was a [i» list Institution. While I itndled under Professor i wbo wm for years man-1 Souis'i band. ifltion, Tompkins was for with th* Great 1 Circus, and bore the dls-I K lhat time of being the t tend leader ever to tra-a major entertainment le hai also had oonaider-riewe In vaudeville thea 1 been on the rko circuit, I hit professional engage-1 with the ''Sunny" jjom- *nd hi* orchestra, 1 rapoasl ble for much of 1 « laat j«rt extrava, »»»Just oompleted a win-»ment at th* Beverly Tor the past three hjv» Played at Lake Other engagement* infer the a. C. ••digs,'' the Pathe studios, and »<lio station*. Parker, who wrote tlie < Shipwrecked," haa been I to direct the singing for "ctioa- Mia* Parker la "•d to do thla work, for ifegulariy ov«r KFi and I rvUo station*, in ad-ft* havin, worked at the WS. for the of "8hj[) "v« been held every dBn»* the la.t week, j, CMt *'|ll be announced Ml Wfet ICE PLANS JAL BANQUET I tor the annual eommerce It are being made under the w of Wilmer Morby, pre*i-I tbe senior class of the Col-■ Commerce, and of the com-I chosen to make arrange-tor the affair, which will be mi n. *« Of the Daily Trojan will Wbuted to the guests on the *• the banquet for the pur-t acquainting the down town 'ith the work of the Col-Commerce, and of provid-Macta for the student* of •w in th# university, following committee* are to ^reparations immediately and t* Wilmer Morby, wt! Karl Waegele, chairman McClung, Alton Oar ^tfi Hyrum White, chair-'<* Mlcciche. *1 lauren Dahl, chairman; ®lth, Robert Dubbell. llJi Doris Sweet, chairman', 1 Todd, Katherine Staub, We, *ti#8t Tom Mills, chairman; ^rc, Harold Barr. ■•r«* Aira; Hugh Mile*, j^enta-, Leslie Pay, chair-**** McClung. """tiow. Walter Hewett, Booth, Otto J«rry Spann. I ■>a,; Marion Kvans. chair-1 **°rat, Merrill I'oore. Don Millican. Tagged Students Face Suspension; Must Meet Dean Traffic violator* who have been tagged by the Knights and have failed to appear at the last two meetings of the court must appear at the meeting to be held in Dean Baron's office, 203 Student l'nion, on Monday afternoon, according to Bud Medbury, committee chairman. Failure to do so will result in Immediate suspension from classes. The traffic committee has noticed that the number of violators has been steadily decreasing since the application of rigid enforcement. The Knights warn students that ail violators of the city traffic ordinance will be tagged and required to appear at the traffic court. 'LONE EAGLE' IN INTERVIEW TO WAMPUS Roar ins Blizzard DENTS ELECT Humanist Logic ASSOCIATED STUDENTS BACK SSE MORGAN RALLS I "S&IZL SHRINE AUDITORIUM BENEFIT W. A. A. TO HOLD AWARD MEETING; SPREAD PLANNED Planning to nominate officers for thi* semester and to award jerseys, the Women's Athletic associ-atlon will give a spread on Mar. 17 ln the gymnasium dancing studios. At the same time the honorary hockey team will be announced and awards for handball, hockey, and swimming given. Tickets for the spread, at 25 cent* each, may be secured from Norma Chapman, senior; Mary Jane Lamer, junior; Vesta Wiley, sophomore; and Mary Rogers, freshman. Tickets will also be sent to all houses. In keeping with the season, the program, arranged by Maxine Johnson and Mary Alice Colt, and the decorations, under the charge of Margerie McPherson, Jean Lit-tle, and Charlotte Small, will follow the Saint Patrick ide*. "Petitions for candidates must be submitted,'' said Janet Hampton, W. A. A. president, "and may be secured from the registrar's office. Those eligible for president must have a grade point average of 1.3. All other offices demand that a candidate have an average of I." Jersey* are given on the basis of recognised credit for participation ln five team sport* and two individual sports. Those receiving the Jerseys have not been announced Impromptu Program Greets New Women Impromptu skits, debates, and other stunts were Included on the program Thursdsy night when women of the Residnce hall initiated ten new co-eds. Glessie Strange, activity chairman, was in charge of the ceremony. Those who were welcomed to the house were Dorothy Leland, lone Law, LaVancha Naftel, Oene Doran, Wanda Waltser, Audrey Gill, Eileen Hamilton, Margaret Giles, Charlotte Moulton, and Phyllis Adkinson. Members of Alpha Eta Rho Accorded Lindbergh Story. Devoting itself to aviation, the new Wampus makes It* appearance late tbls week. An article written from an exclusive interview granted Dick Mugle snd Art Neelley, two members of the Alpha Eta Rho aviation fraternity, by Colonel Charles A. Lindbergh, will appear in the aviation number. With thi* I* included a picture of the famous flying colonel taken just before his epoch-making flight to Paris. "Pieces on Earth," a surprise story dealing with the business depression, has been written by Mary Beth Fyle and Illustrated by Ad-I rlenne Rosso. The feature story is one written by Bryant Hale, who was editor of the Wampus two years ago. It is entitled "The Hope Faith" and tells of the charity benefit that is shortly to be sponsored by the students of the S. C. campus. Lowell Redelings is the author of i "Flight," a love story w oven around | the winning of the prize cup in an air derby. While "Slim Wins the I Derby," is a humorous story by Leo i Duchowney, accompanied by a drawing of Leon Sankoff. A sophisticated airplane story, "Hollywood's Crack-Up," by Charlotte Brown, will appear with illustrations by Betty Talbert. Beatrice Barefax again holds forth, this time devoting her attention to members of the 'Alpha Theta Rho fraternity and other air-minded students on the campus. Carying out almost exclusively the aviation idea, there will be the customary book reviews and more than the usual number of Jokes. CHICAGO. Mar. S.—(UP)—A wild, roaring blizzard, heaviest of the winter virtually turned the middle west Into one ruge snow drift tonight. Cities were isolated, railrosd j trains were derailed and maroon I ed. Roads were blocked. Thousands of automobiles were burled. Tele ; graph lines were weighted dowu and rendered useless. Waves in the great lakes were mountainous, j 8»ven deaths were attrlbu:ed directly to the blinding snow In Chicago. Two were killed In Mllwau kee. AS PRESIDENT Charles Rapp Defeated in Election for of College. President Balkans Hit By Severe Earthquake Dozen Towns Rocked by Shocks; Death Toll Believed 43. BELGRADE, Jugoslavia, Mar. 8 — (I'P)—Severe earthquakes shook large areas of the Balkans today, after earlier temblors Saturday t had caused great damage. A dozen (owns were rocked by work, transferring to the l iilvet Morgan Ralls was elected stu dent body president over Charles Rapp In an election held Friday in the College of Dentistry. The vote cast was the greatest ever ! known in lhat Institution, because of the keen Interest and competition betwcen the various departments and rival factions, with sludents from the Clinic nnd Science-Technlc building participating. The results for the other offices were: Wendall llall, firsi vice-president, defeated Jerome Smith; Barney Faubln, second vice-president, defeated Stan Norton; Herbert Spencer, secretary-treasurer. defeated Walter Zuekerman. Ralls, a Junior In dentistry, id president ol Psi Omega, profes sional d»ntal fraternity, recently elected to membership In Alpha Tau Epsilon, honorary denial fra ternity, member of Alpha Tau Omega, national mocIuI fraternity, at Occidental College, at which in stltutlon lie took his pre-dental | ■'Humanist Logic and Theory of j Knowledge," will he discussed by | Porf. F. C. S. Srhlllcr at the Philo- I sophy Forum tomorrow afternoon j at 4:30 in the Mudd Memorial 1 buiding. The address will be the second by Ihe speaker on Ihe general subject of "Humanism," the name which he gave to Ihe p:ag | mat ic philosophy because of Its em- t phasis upon man as the standard by which the teal Is measured. Professor Schiller Is a permanent PROGRAM, DANCE ON APR. 4 member of the S. C. philosophy staff. He spends the first semester al Oxford university and the second ai Southern California, uhcre his courses this term are in the philosophy of I'lalo and Arls-totle, and In the humanist theory of knowledge. The lecture totum row is open to students and the public. CHARLES MESSICK LECTURES AT S. C. Charles P. Messick of Trenton, N. J., chief examiner of the civil service commission of the state of New Jersey, is to lecture at this university daily during a one-week Institute of Public Administration to be held her* June 8-13, according to the announcement of Emery E. Olsen, director of the School of Public Administration. Dealing with the duties, powers, and responsibilities of those in public office, tbe Institute is to be attended by stale, county, and municipal officers and personnel, including mayors, city managers, commissioners, and citizens actively Interested in public affairs. Session* on the campus will be devoted to public administration, budgets and accounts, streets and highways, public education, recreation, parks, juvenile welfare, and public safety. today's shocks. Peasants fled to the count ryside through torrential rain. The shocks h'*gan at 2 am. and continued until 2 p.m. No loss than 24 severe shocks were registered in the vicinity of Gjevgjell in 8 hours. Heavy underground rumblings accompanied the quake. The death toll was reliably reported to have reached 43. Of these, 19 were said lo have died In the village of Piravo, In Jugoslavia. Piravo was reported worst affected. One dispatch said that the town had been destroyed. New earth shocks were felt In the Jugoslavian districts of Strumitza. Gjevgjeli and Dojrau. Three quakes w hlch yesterday wrecked a wide but sparcely populated area along the frontiers of Jugoslavia, Greece and Bulgaria were followed by recurrent tem blors in Jugoslavia aud southwestern Bulgaria today. Reports from Sofia, capital of Bulgaria, aald that the new shocks were felt at 4 a.m., lasting 30 seconds. Eleven successive shocks were re ported al Gorna and Djoumaja. The population was panic stricken. In Jugoslavia, 1,000 houses were reported demolished al Striraitza and several hundred others were reported razed by the quakes In neighboring villages. Communications were disrupted. One single Held telephone line was operating late today, opening communications lo Slrumitza whence meager report* of the catastrophe came. j sit yof Southern t'alifornln In 1H27. I Presentation of the new officers to the student t od} will be iioiue I lime nexl week at. meetings to be Lunch Dates Of Houses Are Listed Fraternities Again Will be Hosts to Each Other This Week. German University Instructor Criticizes Colleges of America That thc fame of the University of Southern California as an educational institution is becoming practically worldwide is evidenced by an article written by Dr. \V. Seedorf, professor of mathematics and sciences at the University of Goettingen, Germany, and published in the winter number of Visiting Protessor of Italian Culture held In the Clinic and in the Sci ence-Technic building, according lo Flank Conley, retiring president. Inasmuch as Ihe College of Dentistry has its own constitution, elections are held separately from those of the rest of Ihe University The officers newly electd will hold office until next February. T SETS DEADLINE FOR 'BOYS' NIGHT Registration for Boys' night will be accepted only until noon today, those In charge of lhe event scheduled for Wednesday night at u:3u in the V. M. C. A. assert. Plans have been made for lead ers on the campus to meet with boys of "prep" school age at lhe council dinner this week, with ihe guests featured as entertainers. From All-Nations' foundation a boys' harmonica baud will olfer a series of numbers which ia has presented for audiences In theaters of Callfoi nia. A boys' quartet, sing ing without accompaniment will present a program of Spanish American popular songs which have been produced under the direction of the Spanish-American instltuto at Garneda. Dinner will be served at 23 cents a plate. Social fraternity exchange lunch eons scheduled by the interfrntcnt Ity relations committee of the Intel fraternity council for llils week are ns follows: Beta Kappa — Theta Pal. Delta Chi — Tsu Epsilon Phi. Delta Sigma Phi — Tau Delta ! Phi. Gamma Epsilon —Sigma Phi Ep j silon, j Kappa Alpha — Sigma Nu. Kappa Sigma — Zeta Beta Tau. Sigma Chi—Phi Beta Delta. Phi Kappa Psi — Sigma Alpha i Epsilon. Phi Kappa Tau—Sigma Tau. Phi Nu Delta—Phi Sigma Kappa. Pi Kappa Alpha — Alpha Nu Delta. Delta Phi Delta — Alpha Epsilon Pi. I This week's exchange will be the auditorium and a dance will be held at the same time In the Shrine ballroom, B. P. Schulberg. general manager of I'aramounl; Abraham Lehr, general manager of t'nited Artists; Conrad Nagel, t'nlveraal star; Fred Nlhln, M.-O.-M. director, and Clinton Wunder are working through the Motion Picture Academy of Arts and Sciences on the auditorium program . Tickets for 'his entertainment will he priced from *1 .SO. Bids for the dance will R > on sale this week st $2 per couple. COLORFUL FESTIVAL The affair will revive a colorful and spectacular form of entertainment always given for a worthy cause. A kerrness was originally a religious fesllwil of n benefit na ture, which originated in Flanders «hen the village lolk gave danccs on the green for charity. In Amer-I lea II i ii me to nu an any lorm of entertainment, Indoors or outdoors, .liven with nn altruistic motive. In Los Angeles the Iasi kermess v.ns given about 30 years ago In ihe Mason Opera house. Members I of ihe snme families that sponsor-|ed that affair are actively Interest-ill in the Trojan ki miens to be glv-i en In April. The lobby of the Shrine : id I tori uni will be gay and leatlve In keeping with Ihe spirit nf the Kcnuess. The lunds are tn ' :o lo the Associated City Employees commit tee for the relief of the unemployed, with the recommenda* lion tlial the money be used for Ihe relief of unemployed women. BURCHAM IN CHARGE I Joe Burcham is general chairman of the Kermess; others on the ex rcutlve committee Inclui.e Hugh Vndrews, secretary; Bryant Hale, | press representative; Janet Me-Coy, vice-president of the Associated students, who will have charge of coed ticket sellers; Winifred Definite plans for the Kermess, a professional benefit to be ci'en under \ssociatcd Students auspices in the Shrine auditorium on Apr. 4 for thc relief of unemployment, were announced yesterday. The alfair will be the first collcge unemployment benefit in history. Outstanding motion picture stars will appear on Ihe program In the i sixth of the year. Announcement Biegler, secretary of the Associat-of another Interfralernily smoker I ed Students: Arnold Kddy, general will be made soon, according to Fred Chase, chairman of the inter-fraternity relations committee. Speaks at Meeting STUDENT STORE RECEIVES BOOKS the student paper there. In his article, Dr. Seedorf uses two cuts made from picture* which he took at the graduation exercises during the semi-centennial celebration here last June. Dr. Seedorf while studying in America, was delegated to represent the University of Goettingen in particular, and all Gerinon universities in general at S. C.'s semicentennial gathering. Besides making general comments on the life of uni'erslty and college students in America, Dr. Seedorf referred in this article particularly to the presence of athlet- ic* in colleges here. In Germany, they are no departments of physical education in institutions of higher lear ning and no Intercollegiate competition. The professor wrote in his article, "There, football games of the university often bring In unheard of sums of money, the seats for a single game in the great stadiums are sold for as much as ten dollars apiece." Tbe "Goettinger Hoehschul-Zei-tung" In which the article appeared, was sent to Dr. Edwin Mohme, chairman of the German department. Dr. Bruno Averardi of S. C.'s Italian department ls lo be the main speaker at the Town and Gown club meeting to be held in the preBldeul's suite in the Administration building ou Wednesday afternoon. Dr. Averardi, who is a visiting Instructor from the L'niversity of Florence, will talk on "Modern Italian Art." Also speaking on Ihe program is Mr. Samuel Armstrong of Montecilo, painter of murals. The meeting will also feature a loan art exhibit of rare paintings and sculpture. Exhibitors whose art will be shown to member* and guests of tbe organization are Mmes Katherine Leighton, Walter Harrison Fisher, William A- Lyman, Oliver S. Hershman, George O. Robinson, Oscar Trippett. Sydney A. Temple, Burrell O. Raulstou, Hugh W. Harrison, Weuzell, Ernest L. Ipsen. Mr. aud Mrs. Roger Noble Burnham. Samuel Aim-strong, Dr. James H. Cousings, Robert C. Vose, and members of the Stendahl and the Hatfield gal leries. Several books have been placed for rental by the Book Nook in the Student Store as well as three new Star dollar series books being put ou sale. "Sophisticates," a story of a strange woman, by Gertrude Atherton; "The Daughter of Fu Manchu." a continuation of the mysterious Fu Manchu tales by Sax Rohmer; "Shoe the Wild Mare" by Gene Fowler; "The Wooden Woman" by Alexander Townsend which has received very favorable reviews in England and has Just come out here are among tbe new books. Mystery stories abound for those who are interested. Among the Star dollar series can be found: "My Life" by Isadora Duncan; "Liberty" by Ed ward D. Martin, who wrote "The Meaning of a Liberal Education;" "The Glorious Adventure" by Richard liallburton; and "Henry the VIII" by Hacked Kinsteln's "Cosmic Religion" also ls for sale at tbe Book Nook. Committee Meeting of W. S. G. A. Today Irene Bronnals wishes Ihe following girls to meet in the W. S. <;. A. room on the second floor of Ihe Student l'nion at 12:45 p.m. today: Harriet Brown, Lenore Elmore, Mary McKinley, Billie Itodg-ers, Alice Malm, Barbara Bazzard, Gladys Goodsell, Letta Moils, Lydia Barry, Eileen Landers, Eloise Emrlch, and Myra Bush. manager of the Associated Stu dents; and Walter Sykes of the university coordination office. Headquarters for the Kermess are located downtown at 320 Gar-llcld building and all arrangements for the festival will be completed through these offices. JAPAN PREMIER RECOVERS TOKIO, Mar. 9 - (Wednesday) — (I'P) — Premier Hamaguchl returned lo Ills official duties today, completely recovered from bullet wounds inflicted by a young man who attempted tn kill him In Ihe railroad station here several months ago. Law Review Contains Discussion Of Federal Oil Lands Reservation Law of Discovery in the Courts of California" by Dudley M. Harkclroad, "The Current Federal Oil Policy,'' by Charles <>. Haglund, and "Responsibilities and Liabilities of the Transfer Agent and .Registrar" by Fredericks H. Beh-renils and Sheldon D. Klliott, research assistant to lJrof. Robert Kingsley, an- featured articles .nation Is well supported by prac- in the current issue of the Southern California Law Review, pub lished periodically by the School of Law. Of probable interest In the oil industry in the state of California Is Ihe article of Charles G. Hnglund of the North Dakota bar, concerning the federal policy of reserving certain government oil lands from private ownership. The writer quotes the laws of a number of Latin-America countries to Indicate tlce elsewhere." Commenis on various phases of law by members of the editorial staff of the Law Review, case notes, book reviews, and a restatement of the law of contracts with California annotations complete the contents of the latest number of the legal magazine. The Southern California Law Review is published in October, December, February, April, and June by the faculty and students of the Trojan Law School, that "the change of policy In the; with Prof. Robert Kingsley as edl-I nited States of reserving lhe min lor-ln-chief and George Henderson, erals in the public domain to the I student editor. KERMESS BACKED BY CIVIC LEADERS OF LOS ANGELES Sponsors ol thc Kermess Include 1 civic leaders, motion picture exe uitlves, business men ,and prominent women club workers. They are as follows: | Lawrence Barker, president of liaiker Brothers'; Harry J. Bauer. I president of Ihe Automobile Club of Southern California, director ot ihe California bnnk and of Pacific Mutual, and a trustee of (lie university; Mrs. C. Raymond Iliad ford, director of Ihe Legal clinic; Mrs. Arthur Buermlller, president of the Assistance league. Judge Georgia P. Bullock; Abia ham I.chr, general manager of 1'nlted Artists, and Mrs. Lehr; Orra E. Monnette, vice-chairman of the board. Bank of America: Conrad Nagle, Universal star; Fred Niblo, Metro-Goldwyn-Mayer director, and Mrs. Niblo; Emory R. Olson, co-oidinatlon officer of S. C. and president of Ihe Los Angeles Advertising club. Mrs. Murrell O. Raulston, promt neut club woman; Mrs. B. P Schulberg. wife of th* general mansger of Paramount Pictures; Miss Grace S. Stoermer, vice-president of Ihe Bank of America: Miss Anne Patton; Mrs. Fiancls I*. Graves; Mrs. John C. MscFsr-lund. noted child welfare worker; President and Mis. It. B- vou KleinSmid; Mr*. Thomas G. Winter, chairman of the Roard of lie view, Central Motion Picture bur eau, former president of the Federated Women's Clubs of America. In addition to the above spoil sois, muny others have endorsed Ihe Kermess and are expected to back It. Among these are Mayor John C. Porter, J. A. H. Kerr, president of the Chamber of Com merce, and I>eItoy Ow en, president of the Junior Chamber of Commerce. NEW TROJAN CLUBS FORMED IN STATE Trojau alumni have been espec tally active in organization during the past few months. San Francisco men formed a Trojan club recently in which alumni from all the cities of the Ray district are included. Craig Smith is president. The club has a luncheon meeting at the St. Francis hotel the first Thursday of each month. Ou down the coast, the Ventura county Trojans, both men and women, have organized a joint club, with Charlea Wright a* president Bob Beardsley as vice-president, and Vera Everett as secretary and treaaurer, Huntington Park men organized early in February and are at present carrying on a membership campaign. March li. Alhambra alumnae will meet for the formation ot an Alhambra women's club, U> include Trojana living ln Alhambra. Covina, Azusa. Arcadia, Sierra Madre, San Gabriel, San Marino Rosemead, Puente, Monrovia, Glen dors, and El Monte. Plans for the formation of otlier Trojan clubs are under way witb the Alumni office backing alumni in the different communiiies in their desire tor •vganisad ftojau activity.
Object Description
|Daily Trojan, Vol. 22, No. 102, March 09, 1931
|Format (imt)
|Full text
Irooeo staff (, M.0
ir, ,.keJ '0 se* J# y tod«y •" th* *"nu
Los Angeles. California, Monday, March 9, 1931.
jhestra is
I0SENFOR IllSIC SHOW
Hy Tompkins Will ct B 0 b Brown's danders.
lh» ipcond successive year, f Tompkins will direct Bob orcbeitra when "Shlp-tbe annual extrava-1 presented in Bovard audl-17 and lg.
Jlni *HI be remembered I wcoesiful wielding of the rt last year s musical com-»bu bid quite a little bit »no« in musical work, I been conductor of the Jimi bund while he was a [i» list Institution. While I itndled under Professor i wbo wm for years man-1 Souis'i band.
ifltion, Tompkins was for with th* Great 1 Circus, and bore the dls-I K lhat time of being the t tend leader ever to tra-a major entertainment le hai also had oonaider-riewe In vaudeville thea 1 been on the rko circuit, I hit professional engage-1 with the ''Sunny" jjom-
*nd hi* orchestra, 1 rapoasl ble for much of 1 « laat j«rt extrava,
»»»Just oompleted a win-»ment at th* Beverly Tor the past three hjv» Played at Lake
Other engagement* infer the a. C. ••digs,'' the Pathe studios, and »
|Archival file


text:
Small hobby breeder in Central New Jersey. Our dogs have all orthopedic and eye clearances. We breed "English type" labradors, and puppies are raised in our home. Orthopedic soundness and sweet, laid back temperaments best describe our labradors. We are active in conformation, obedience and hunting. Please visit our website to view photos of our dogs, their accomplishments, and for contact information. I am a current Board Member of the Mid Jersey Labrador Club, a member of the National Labrador
Club and have judged at several Labrador Retriever Specialty shows.


text:
ICNA Condemns Ban on Minarets in Switzerland
JAMAICA, New York (November 30, 2009) – The Islamic Circle of North America condemns the ban on the construction of minarets in Switzerland. We are exceedingly concerned with the brazen cultivation of fear by the Swiss People's Party and its obvious effect on the Swiss public.
The minaret has long stood as a cultural symbol of Islam, the prohibition of which is indicative of intolerance and suppression of religion. Whereas Western nations have constantly been at the forefront of the struggle for religious and civil rights, the move to ban minarets in Switzerland can only be seen as a regression in these efforts. By upholding this ban, the Swiss government will not only deny its Muslim citizens their basic religious freedoms, but also encourage those parties whose sole agenda is to sow seeds of discord amongst citizens of the world.
Dr. Zahid Bukhari, president of ICNA, said in a statement, "Right wing extremism is on the rise in European media and political arenas. This is affecting public opinion and giving way to an extremely dangerous trend that has begun to threaten harmony in European, including Swiss, societies. Intolerance must be condemned by everyone, including and especially all civic, religious and political leadership in Europe."
Religious extremism and blatant fear mongering cannot be allowed to prevail over reason and justice. Western democracies must continue to rise above these threats and remain diligent in their promotion of tolerance and understanding.
ICNA encourages political and community leaders to take a stand against this ban and to persist in their work towards the freedom of religious practice. We encourage all Europeans and European Muslims to contest this law and act as a collective voice of dissent against this deliberate act of religious discrimination.
The Islamic Circle of North America is a leading American Muslim organization dedicated to the betterment of society through the application of Islamic values. Since 1968, ICNA has worked to build relations between communities by devoting itself to education, outreach, social services and relief efforts.
Islamic Circle of North America
Office: (718) 658-1199


text:
by Rene
Medusa Costume Photo 7 of 10
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This homemade costume for women entered our 2015 Halloween Costume Contest.
A word from Syreeta, the 'Medusa' costume creator:
I have to come right out and admit that I can't take complete credit for this one. The design, styling, and sewing was all me, but the idea was from a little friend… literally. I work... Read more »
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A wedding ring is sometimes the only jewelry that a man wears, so why not make it count. All these rings are modern and minimal, but with a twist. Some of these unique wedding rings even come in unusual materials such as carbon fiber (black), whiskey barrel wood, antler horn, meteorite, and even recycled shotgun metal - an amusing nod to the old school "shotgun wedding." While … [Read more...]
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Hello to all!
So I have a 1977 Batavus moped. Runs on a 48 cc 2 stroke engine. I have some questions regarding the engine and swapping it. First question is, can I put any engine on the moped or only a specific one. Does it only hold 2 stroke engines or can I put a 4 stroke? Another question is, what's the biggest engine I can put on it without adding any modifications. Just bolting on and bolting off. Would it have to be a 48cc or can I put a 2 stroke 80cc and be fine? Would I have to put another carberator or can I leave the original one? How would I go about swapping the engines? Very handy and can do it, I just need that General information and steps to get it done. Thank you!


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Tony Blair's Sister-in-Law Calls for the Liberation of Jerusalem
Tony Blair's Sister-in-Law Calls for the Liberation of Jerusalem
Lauren Booth, sister-in-law of former British Prime Minister Tony Blair, has called for the liberation of "Al-Quds" [Jerusalem].
"We say here today to you, Israel, we see your crimes and we loathe your crimes. And to us your nation does not exist, because it is a criminal injustice against humanity," she declared at an anti-Israel rally in London Trafalgar's Square.
Booth added, "We want to see Lebanon, Jordan and Egypt go to the borders and stop this now. Liberate Al Quds! March to Al Quds!"
The 44-year-old, who illegally sailed to Gaza on a flotilla boat, converted to Islam in 2010. She said a holy experience in Iran was the cause for her faith conversion.
Great Britain's Daily Mail reported that Blair's sister-in-law now wears a hijab, covering her head whenever she leaves home, no longer drinks alcohol, and visits a mosque 'when she can.'
Booth said, "The strange thing is that since I decided to convert I haven't wanted to touch alcohol, and I was someone who craved a glass of wine or two at the end of a day."
According to Booth, she had a religious awakening during her visit to a shrine in the Iranian holy city of Qom.
She felt a "'shot of spiritual morphine, just absolute bliss and joy," she told the Daily Mail, adding, "I always felt that the ummah [Muslim community] is a very loving, peaceful place and I am proud to be a member of it."
A journalist and human rights campaigner, Booth works for Press TV – the English-language Iranian news channel.
The Trafalgar Square rally could be one of the London's last as the mayor of Britain's capital city vowed to constrain unrest as the 2012 Olympics approach.
"The Mayor believes that intolerance of our fellow citizens and hate crimes against specific communities are totally unacceptable, particularly in a city like London and especially in 2012 when the eyes of the world will be on the capital," said a spokesman for Mayor Boris Johnson, the London Jewish Chronicle reported.
According to the spokesman, the Greater London Authority, the city's administrative body, will not authorize any political rallies during the Olympic and Paralympics Games.


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Took some pics of Beau and Takoda today.
Takoda is showing some potential as a duck hunting dog too Any time she hears a gun shoot, she's either hitting the end of her lead or the fence of her pen to go retrieve what was shot. Whether it be a plywood or a duck, she wants to fetch it. So we'll see how this works out....there might even be some coon hunting in her future
Excuse the bad quality, these were taken on my phone:
She's not too shabby looking for a lab, if I do say so myself
He's finally starting to show rib now, which means he's finally back where I want him for the summer
Can you tell they're best friends?
Yeah......she's DEF. not very bright
And he's well....yeah he just HAD to move.....


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We recently finished our kitchen renovation. It had been mostly done for a long time, but we put the frosting on the cake this summer by completing the back splash. We opted to go with a simple white subway tile. I'm enjoying the clean, sleek look it adds to the kitchen.
Here are some before shots. These were taken before we moved in. We thankfully had 1 month to start our renos before we moved into the house. This kitchen was the first thing to change. It just wouldn't have worked very well for us - no dishwasher, single sink, no upper cabinet doors (I like "stuff" out of sight behind doors), very little counter space...
After shots complete with the new backsplash
We reconfigured the kitchen layout by adding this peninsula with a high bar top which adds more cabinet & counter space. It also doesn't crowd the fridge & stove so much.
|Our control centre
|Here's a better shot of my Paris gallery - B&W photos from a calendar &frames fromIkea - easy peasy.
Paint: Camalot by Benjamin Moore
Cabinets, countertops, hardware, light fixtures: Ikea
Flooring: Floor It
Backsplash Tile: Home Depot
If you'd like to see our living room makeover click here.
If you'd like to see our main bathroom makeover click here.
Well, I'm off to start my fall decorating. I'm warming to the idea of Fall arriving & my oldest had his first day of Grade 3 today. My little girl starts Kindergarten at the end of September so I have some extra time to cherish with her. I hope you'll join me next week when I link up to Nest of Posies Fall Festival. September promises to be a month full of beauty & inspiration.
Enjoy your day!


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Hot Gists
Pakistan Warns Pot-bellied Cops To Diet or Quit
June 18, 2012 – Pakistan Warns Pot-bellied Cops To Diet or Quit
A Pakistan police commander has ordered tens of thousands of pot-bellied officers to diet or quit frontline duties, officials said Monday in what one newspaper dubbed the "battle of the bulge".
Habibur Rehman, police chief in Pakistan's most populous province Punjab, has ordered 175,000 personnel not to allow their waistlines to exceed 38 inches (96 cm), spokeswoman Nabila Ghazanfar told AFP.
"I'm on a diet and if I can do it, why can't you?" she quoted Rehman as telling officials last month in the province.
At least 50 percent of Punjab police are overweight, Ghazanfar said.
Local daily The News said the number overweight officers in the city of Rawalpindi, the headquarters of the army that has been hit by numerous Taliban attacks, stood at more than 77 percent, adding that "policemen appear to be losing the battle of the bulge".
Police said officers had been given until June 30 to shape up and those deemed too fat from July 1 would not be given jobs in the field.
"Police officials are joining gyms, jogging and doing other exercise, including a lot of running to become thin and slim," Ghazanfar said.
Overweight officers are ineffective and "cannot chase bandits, robbers and other criminals properly", she added, blaming the problem on under-staffing.
"We have a shortage of personnel, what can you expect when one official is doing the job of six people? They don't get time for physical fitness," she told AFP.
Another official who did not wish to be named said 100 stations were initially singled out for the exercise order, but Rehman later decided to send warning letters to the entire Punjab police force.
He said the chief believed people only had pot bellies "if they commit a sin or if they are sick".
"It is my guess that the department will assess progress and if necessary the deadline may be extended," a senior official said, requesting anonymity.
The Pakistani diet is rich in meat, oil and ghee.
The country is ranked 165 of 194 on the Forbes list of fattest countries, with 22.2 percent of the population considered overweight despite immense poverty faced by millions in the country.
Local reports have put the figure at 25 percent


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New York Gov. Andrew Cuomo's budget must address $1 billion gap
ALBANY -- When Gov. Andrew Cuomo releases his 2013-14 state budget plan on Tuesday, a new round of cuts and new sources of revenue are likely to be part of the package.
That's because New York faces an estimated $1 billion budget gap in the coming budget year, when overall spending is expected to total around $132 billion. The gap comes on top of a staggering array of budget problems, from the anemic economy to the impact of Hurricane Sandy. Spending on education, Medicaid and public employee pensions and benefits are all headed upward, making it harder to cover government spending at a time when tax revenues are lagging.
"He's got to pull some rabbits from a hat," said E.J. McMahon of the Empire Center, a group that advocates for lower taxes and spending in New York. McMahon said that unless Cuomo's budget projections for the year to come are proven to be wrong, "he has to do an additional bit of cutting and restructuring in order to avoid a tax increase."
On the flip side, Cuomo's new budget could include projections of significant new revenues from the Las Vegas style casinos voters will be asked to approve in November. Also on the table is Marcellus Shale gas hydrofracking, which could pump hundreds of millions of dollars into government treasuries if the state Department of Environmental Conservation permits it to occur. By some estimates, fracking has produced close to $2 billion in new tax revenues in the state of Pennsylvania.
Friday, Comptroller Thomas DiNapoli reported end of year state tax revenues were above what they were at the same time in 2011, but are nevertheless running below expectations.
"Tax collections are still not growing at the rate needed to meet year-end projections, and the boost in personal income taxes in December likely won't continue," DiNapoli said, referring to an end of the year rush by wealthy taxpayers to sell assets and avoid new Obama taxes that took effect Jan. 1. "So far the budget has been balanced by spending less and taking advantage of one-time windfalls. With the budget process about to begin again, revenue projections should be realistic so that the budget is not balanced with revenues that won't be there."
In a memo sent out this week, McMahon described a dire situation facing New York government finances.
"Assuming school aid, Medicaid and employee health insurance are treated as politically off-limits to cuts, and assuming the governor holds the line on both taxes and fees, the only way to close the budget gap would be by making a 3 percent across-the-board reduction in absolutely everything else," he wrote. "If the entire local assistance budget -- including higher education, mental hygiene and transportation -- was also held harmless (from cuts), the gap could be closed by cutting state operations spending by 14 percent."
Under current budget plans, school aid is set to grow by $853 million, Medicaid by $653 million, state worker healthcare and pension costs by $675 million, and debt service by $315 million, the Empire Center reported.
DiNapoli said that Tuesday's presentation should answer some questions about how Cuomo intends to plug the holes in the budget.
He noted that the Cuomo Budget Division's mid-year update issued Nov. 28 "projected tax growth of 2.9 percent for the year. However, even with higher than anticipated PIT collections in December, All Funds tax collections of $46.4 billion rose just 1.2 percent, or $545.4 million, from the same period last year."
"In order to meet current year-end projections, tax collections will have to grow 7.1 percent in the last three months of the fiscal year," DiNapoli added, referring to the period from January-March 2013. "Current projections also do not include estimates of the potential budgetary effects of Hurricane Sandy."
The current fiscal year ends on March 31 and a new budget is supposed to be in place by April 1. State legislators are aiming to pass the budget a week early, since both Passover and Easter week begin during the last week of March. They are scheduled to recess on Thursday, March 21. The Assembly is scheduled to return April 8 and the Senate on April 15.


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The Subaru BRZ was built for driving pleasure, not high-speed statistics. It's still interesting to watch one reach a top speed of 147 MPH. But was this run illegal?
The video's uploader Speedracer38 states that the run was performed on the Autobahn, which everyone knows is unrestricted in many places. Speedracer38's only other video that references the German highway is clearly in the US, and the description states, "we were driving on the autobahn in Europe..... ;)".
One day before Speedracer38 uploaded his video, another YouTube video showed a BRZ hitting roughly the same speeds (235 kph or 146 mph) while showing a bit of the road. That run clearly was shot on the Autobahn, but as for the one made by Speedracer38? We're more than a little skeptical.


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Stephen will soon have some competition at Ultra!
Alexa Vega will make her Tomorrow People debut in the Jan. 22 episode and TVGuide.com has the exclusive first look.
The Spy Kids star will play Hillary Cole, a new recruit who goes head-to-head with Stephen (Robbie Amell) to see who will make it to the next stage of agent training. Unfortunately, the loser gets stripped of their powers, and Hillary, who's basically the paranormal version of Tracy Flick from Election, will stop at nothing to win.
Tomorrow People: Is Ultra really evil?
"She beats the crap out of me," Amell tells TVGuide.com. "She messes Stephen up. She's conniving. She's doing anything she can for herself. She does not care about the people around her."
Tomorrow People airs Wednesdays at 9/8c on The CW.
(Full disclosure: TVGuide.com is owned by CBS, one of The CW's parent companies.)


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NumisWiki For the New Collector
Ancient Greek Coin Collecting 101
|The Bellovaci, among the most powerful and numerous of the Belgian tribes of north-eastern Gaul, were conquered by Julius Caesar in 57 B.C. The name survives today in the French city of Beauvais, called by the Romans Caesaromagus. The Bellovaci territory extended from modern Beauvais to the Oise River, along the coast. When Caesar learned the Bellovaci intended to conquer the territory of their Suessiones neighbors, he decided to oppose them and prove Roman superiority. The Bellovaci were surprised by the arrival of Roman troops but, despite his force of about 30,000 men, Caesar was intimidated by the size of the Bellovaci forces. Neither initiated battle. The Belgic warriors set traps in the woods for Roman foragers. Caesar called for reinforcements and built a bridge across a marsh to position his troops within range of the Bellovaci camp. The Bellovaci retreated and then attempted an ambush. Caesar learned of their plan and had reinforcements ready to attack, but the Bellovaci were defeated and their general Correus killed, even before he arrived. After the battle, the Bellovaci were impressed by Caesar's clemency but some of their leaders fled to Britain.


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Cheryl Burke, Giles Marini, And More Dancing With The Stars Alumni Attend Simon G's Jewelry Summer Soiree
Pictured above is Cheryl Burke and Giles Marini! Both were seen working the Red Carpet last night at Simon G's Jewelry Summer Soiree and the kickoff of Carnevale Nights at The Palazzo Casino in Las Vegas. Whoa, this reunion had it's way of waking me up this morning. 😯 Other amumni of Dancing With The Stars also attended this event such as Lisa Rinna, Audrina Partridge, Pamela Anderson, and Stacy Kiebler. You can see more pics of them all at the Daily Mail and Zimbio Pictures.
ADDING: And thanks to @DwtsGossip for pointing us to this tweet this morning;
#DWTS @cherylburke tells me she would def. do All-Stars show (dancers haven't been announced) but only with #Gilles pic.twitter.com/LxbJPsRd
— Mike Doria (@MikeDoria) June 3, 2012


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Lives are being changed by the day as a result of people using our hemp-infused, plant-based products! PrimeMyBody is in the business of helping people and the opportunity for you to be first, has never been better. Join the thousands of PrimeMyBody affiliates who are building a business and changing people's lives for the better here in the United States, and soon, the rest of the world!
Watch as our Leadership Team discusses PrimeMyBody's mission and history, global expansion plans, and the many ways you can earn an income by sharing our products with others.
We're in constant pursuit of discovering the best version of ourselves and sharing those discoveries with all who are on a similar path. We recognize how much effort and dedication must be applied to achieve success, that's why our team has put systems in place to help you build an ever-growing team. Come join our mission to live wellness & create lifestyle!
A person can become a PrimeMyBody Affiliate by purchasing the Business Center for $39.00 annually, which includes access to your personal business center, replicated website, and online marketing tools. No product purchase is required. Earnings or potential earnings of existing Independent Affiliates are not necessarily representative of the income a PrimeMyBody Affiliate can or will earn through their participation in PrimeMyBody. No one can be guaranteed success as a PrimeMyBody affiliate.


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Emergency Transportable Housing
A supplement to the standards that will address temporary housing provided in disasters and emergencies
- [Links to any documents available for public comment]
- About this Rulemaking
- Frequently Asked Questions
- Background
The Board has issued guidelines for temporary housing provided by the government in emergencies and natural disasters. Emergency transportable housing units, which are designed and manufactured for transport over roadways, have a smaller footprint than other types of housing and pose unique accessibility challenges. Access to such housing was found to be problematic in the aftermath of hurricanes Katrina and Rita in 2005. The new guidelines supplement the Board's ADA and ABA Accessibility Guidelines by adding provisions and exceptions that specifically address emergency transportable housing. The Board developed these guidelines according to recommendations from an advisory panel it organized, the Emergency Transportable Housing Advisory Committee, which included representation from disability groups, industry and code groups, and government agencies. Once these guidelines are adopted into the ABA Standards by the Department of Housing and Urban Development and into the ADA Standards by the Department of Justice, they will be enforceable standards.


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Team:METU-BIN Ankara/Project
From 2011.igem.org
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Algorithm

Algorithm

Construction of Relational Database

Construction of Relational Database

Scoring

Scoring

Results

Results

Revision as of 15:37, 21 September 2011
While the interest to the field of synthetic biology is uprising, iGEM and Parts Registry is getting more important for researchers interested working in the field of synthetic biology. As the number of attendees and teams to iGEM competition increases each year, number of BioBricks submitted to the Parts Registry are increasing too. As we all know, Parts Registry is an inimitable library for synthetic biologists. Although it is an important source of information and tool for biologists, the parts are not organized very well. Therefore, while constructing devices, synthetic biologists usually faces following difficulties at the pre-experimental stage:
- Searching through the Parts
- Deciding if the parts needed are already in the database or not
- Deciding which BioBrick will work effectively with which
- Finding the most accurate combination between the parts
- Constructing the most effectively working devices
Figuring out all these answers is time consuming and takes too much effort. Our main goal as 2011 METU-BIN iGEM Software team was to provide a web based tool that helps synthetic biologists at the pre-experimental step to search the Part Registry with input and output keywords and to design their genetic constructs using the biobricks provided in the 2011 distribution of DNA constructs.
M4B: Mining for Biobricks is developed to aid synthetic biologist. Our software proposes a method which can be utilized in pre-experimental step as a supporter data mining tool. Following the user defined input and output parameters, M4B lists all possible composite devices and ranks them according to the novel scoring matrix developed by METU-BIN.
Main goal of our 2011 project is to provide a web based tool that helps synthetic biologists at the pre-experimental step to design their genetic constructs according to their input and output parameters.
- A network of all bioparts in 2011 distribution will be generated, which describes the functional relations between the subatomic bioparts.
- A search algorithm will be developed to reveal all possible device combinations for the user defined input and output within bioparts of 2011 distribution.
- Visualization tools will be applied for graphical representation of the results.
- A web-based user interface will be provided for the developed software.
Extraction of Relations of Bioparts and Construction of Relational Database
Parts Registry contains various types of biobricks and connections in between parts. In our software, we aimed to develop an algorithm which goes over the devices and finds connections between biobricks to present users all possible constructed devices. However, it was very difficult to go over biobricks and find the relations in not very well organized Parts Registry. For that reason, we have decided to form a "Part Connections Database" by examining all the bilateral relations between biobricks which will be used by our program, M4B. By this way, the complex information in Parts Registry will be simplified and our algorithm will work faster and more effectively.
The biology group focused on all of the four 384 well plates of 2011 distribution and extracted information between connections and devices in a standard way. This information is stored into a relational database and enables queries from remote computer programs.
Our software group developed a toolbox to help biology group in storing the part relations into the new database of connections between biobricks. Using this tool, we were able to minimize the possibility of mistakes while entering bilateral relations manually. More importantly, this organized the database entries in a standardized way, according to the requirements of Mining for BioBricks to run the searching algorithm easily.Figure 1: The toolbox which was used while building the "Part Connections Database" by our biology group. Figure 2: A screen shot from our "relational database"
As there are various types of biobricks in the Parts Registry, we set up rules to organize the "relational database" in an appropriate way.
The most important rule is "A constructed device formed by combination of promoter-ribosome binding site-gene-terminator, respectively, is the minimal functional device."
This is the major rule, because without these 4 major biobrick types, a device cannot work. In addition to this, without knowing the description of "basic device" it is impossible for us to present accurate results to users. Although Parts Registry contains various types of biobricks, in our database there are 4 basic biobricks which are promoters, ribosome binding sites, genes, and terminators. This helped us to simplify the complex information in the Parts Registry in our initial attempt of mining.
In addition to 4 basic biobrick types, there are inputs and outputs which are our software's parameters. Inputs are chosen according to promoters. Both activators and inhibitors of promoters were listed in our database. Main source of activators and inhibitors was the information from the Parts Registry. The reason of listing inhibitors was to filter out constructed devices which do not produce the required outputs. When a constructed device is formed by multiple devices, although the input activates first device's promoter, it may inhibit other promoters of following devices. Outputs were chosen according to the genes, the product they synthesize. Although, most of the time, input and output information was extracted from Parts Registry, sometimes external sources were used as well because of the uninformative part descriptions at Parts Registry. External sources such as NCBI, PubMed and wiki of the team who submitted the biobrick to iGEM were used.
Constitutive promoters are entered without any input, but especially their "constitutiveness" is recorded as a property in our relations database.
In our software, after constructing a device by combining biobricks to construct multiple devices, combination between devices is formed according to this rule: "Output of the first gene will be the input of the following promoter(s)"
According to this rule, first promoter will be activated by given Input and the product of first gene will be used as activator of the following promoters. By activation of the promoters, genes will synthesize the output. By this way, the constructed complex is expected to work effectively.
In Parts Registry, there are composite parts, translation units, generators, reporters, inverters, intermediate devices, and signaling devices. As Parts Registry 2011 distribution was being examined in detail, to simplify these parts, their connection information was simplified to promoter-ribosome binding site-gene-terminator relation by our biological knowledge. This rule is explained with details as seen below:
- Reporters
The biobricks which synthesize fluorescent proteins are classified as "reporters" as they give out light to report something. We accepted these parts as "genes" because they are synthesizing proteins.
- Translational Units
Combination of ribosome binding site and gene is classified as "translational units". We accepted this as two biobricks rather than one. Therefore the bilateral relation between ribosome binding site and gene was entered in our database.
- Inverters
Inverters were challenging parts; they are formed from more than 2 biobricks. Rather than accepting this as one biobrick, we accepted this part as a combination of four biobricks. Therefore, we entered bilateral relations between each biobricks as ribosome binding site-gene, gene-terminator, and terminator- promoter. We also entered gene-output and input-promoter connections.
- Composites
- Genereators
- Signalling Devices
- Intermediate Devices
Composite parts, generators, signaling devices, and intermediate devices were also challenging parts like inverters. They don't have a specific rule about how many and which types of biobricks they consist of. However, we accepted them as complex subdevices and entered bilateral relation between each connected biobricks.
In METU-BIN 2011 project, our main priority was to focus on (minimal) Functional devices. Therefore we named DNAs, RNAs, and Tags as "accessories" because they are not vital for a device to work. However, we know that using these accessories during construction helps devices work more effectively by adding some extra features. For that reason, it's in on future plan to form a database for these accessories and present them to our users in their query results.


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BackgroundThread plugin
compile "org.grails.plugins:background-thread:1.6"
grails install-plugin background-thread
DEPRECATEDPlease use the Executor plugin instead
BackgroundThread PluginThis plugin provides your application the ability to throw off background work. It can be used to provide some asynchronous processing in response to key events: the code originally evolved out of parallel web service calls as triggered by a Quartz job. Work is processed by a fixed-size thread pool.Beyond the inherent difficulty of concurrent programming, Grails had a few other difficulties: most significantly, the Hibernate session is bound to the thread, and so threads need to do their own Hibernate session management. This plugin takes care of those issues.It is released under the same terms as Grails itself (the Apache 2 license).
grails install-plugin background-thread
Provided FunctionalityThis plugin provides a service called "backgroundService". As of now, it provides a single method, "execute", which takes two arguments: the name of the work to be executed (an arbitrary label) and a closure which performs the work.An example usage might be this:
If you have a Runnable kicking around that you wanted to run as some background work, you can access the underlying implementation this way:
class FooService { boolean transactional = false def backgroundService def serviceMethod() { (1..<10).each { cnt -> backgroundService.execute("Logging '$cnt' asynchronously", { log.info("${cnt}") }) } }
class BarService { boolean transactional = false def bgThreadManager def serviceMethod() { (1..<10).each { bgThreadManager.queueRunnable(new MyRunnable()) } }
- Almost everything you touch in Groovy is not thread-safe, so you need to pass as little information into your closure as possible.
- You will almost certainly need to crank up the maximum number of connections for your datasource, assuming your background work is talking to your database.
- Groovy will hold onto your entire surrounding context when you have a closure, so if you are memory-sensitive, try to consume as much of it as possible to keep your memory footprint down. This means that it is better to use a consuming iterator (an iterator where you call #remove()) than to use Groovy's built-in functional iterators (e.g. #each{}) More at the plugin author's blog.
Config ExampleThe following snippet can go into your Config.groovy: the values are the default values for those properties.
backgroundThread {
queueSize = 1000 // Maximum number of tasks to queue up
threadCount = 5 // Number of threads processing background tasks.
tasksPerDrain = 100 // See Note
- tasksPerDrain: In concurrent systems, the point of hand-off often becomes a throughput bottleneck. So when one of the background processing threads runs out of work to do, instead of drawing just a single task from the queue, it may opt to drain the queue. This property controls the maximum number of tasks the thread will drain at a time.
Spring BeansThese beans are provided for your use, and can be overridden in the
- bgThreadFactory: A ThreadFactory used to generate new threads.
- bgExceptionHandler: A Thread.UncaughtExceptionHandler that is used to handle otherwise uncaught exceptions in the default bgThreadFactory.
- bgQueue: The java.util.concurrent.BlockingQueue which stores the tasks to be run.
- bgThreadManager: The BackgroundThreadManager that manages the background threads and processes.


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This powerful formula combines the best of science and enzyme technology for degrading fibrinolytic protein compounds produced during inflammation. The carefully chosen herbs have lipooxygenase (LOX) inhibition, COX 2 inhibition, only mild COX 1 inhibition (to avoid blood thinning and stomach sensitivity), as well as free radical protection.* The proteolytic enzymes not only break down inflammatory proteins including kinins and fibrin but also enhance the breakdown and removal of damaged tissue improving lymphatic drainage. Inflammatone is safe to use in high doses yet powerful enough to provide results with as little as two capsules per day. For best results, this product should be taken on an empty stomach, approximately one hour before meals or several hours after.
As a dietary supplement, take two capsules per day between meals, or as directed by your health care practitioner.
Serving Size: 2 capsules
Servings Per Container: 60
Serving Size: 2 capsules
Servings Per Container: 60
Not recommended for use by pregnant or lactating women.