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Journal of Cell Biology & Cell Metabolism Category: Clinical Type: Research Article
Effects of the Hypoxia-Mimetic Agents DFO and CoCl2 on HeLa-Fucci Cells
- Baptiste Bedessem1*, Marie-Paule Montmasson1, Malika Hamel1, Françoise Giroud1, Angélique Stéphanou1
- 1 CNRS, Laboratory TIMC-IMAG/DyCTIM, Grenoble, France
*Corresponding Author:Baptiste Bedessem
CNRS, Laboratory TIMC-IMAG/DyCTIM, Grenoble, France
Tel:+33 0456520045,
Received Date: Feb 13, 2015 Accepted Date: Mar 12, 2015 Published Date: Mar 26, 2015
The Fucci system is a promising model to study the cell cycle in vivo and in vitro. Because it enables to follow the progression of the cycle using fluorescent microscopy, it can be used to precisely visualize the effects of external strains on proliferation. However, it was reported that the lack of oxygen strongly affects the fluorophores used in the HeLa-Fucci cell line. As a consequence, it limits its use in hypoxic conditions. To study the effects of hypoxia on cell proliferation by using this cell line, one could use HiF-1α inducers, such as CoCl2 and DFO, which are often used as hypoxia-mimetic agents. As these iron-chelators also have anti-cancer properties, their biological effects were studied in various experimental conditions. A great variability of actions were reported. In this study, we investigate for the first time their effects on HeLa-Fucci cells proliferation dynamics. We find that CoCl2 has a biphasic effect on the cell cycle, by promoting or inhibiting the progression into the G1-phase. In presence of DFO, we identified an arrest in the G2-phase, which follows a switch-like behavior. These observations bring original results since iron-chelators are mainly known to induce an arrest at the G1/S transition. They also provide specific information about the possibility to use Fucci cells to study the influence of hypoxia on the cell cycle. Finally, by considering the known genetic characteristics of HeLa cells we propose a hypothesis to explain the particular response of Fucci cells to DFO and CoCl2. The discussion proposes a plausible action of DFO and CoCl2 on the MAPK pathway.
The Fucci (Fluorescent Ubiquitination-Based Cell Cycle Indicator) cell is a recently created line of HeLa cells (cervix carcinoma) [1]. This system uses a couple of oscillating cell cycle proteins, cdt1 and geminin, respectively labeled with monomeric Kusabira Orange 2 and monomeric Azami Green. As a consequence, the G1, S, and G2/M phases are labeled with different colors. This cell line is useful to quantify the duration of each phase of the cycle, and to determine the percentage of cycling and non-cycling cells in a non destructive way. Notably, it can be used to visualize in vivo the progression of the cycle [2,3]. In vitro, it is a good tool to observe the effects on cell dynamics of various environmental strains, such as hypoxia. The study of the cellular and molecular effects of hypoxia is one of the important challenge of modern biology. Indeed, it is well established that tumor cells undergo chronic hypoxia, due to the limit of oxygen diffusion in the cancer tissue [4,5]. This chronic hypoxia correlates with an increased resistance to chemotherapy [6,7]. Notably, hypoxia activates anti-apoptotic pathways [8] and can induce proliferation of cancer cells. Indeed, even though hypoxia is known to induce cell cycle arrest in G1 phase [9], it was shown that chronic hypoxia can also stimulate proliferation in various cell lines [10-13]. In a general way, it seems that a balance exists between death and survival/proliferating pathways, depending on the duration and severity of the hypoxic event [14]. The complexity of the cellular response to hypoxia is controlled by the HiF-1α factor, which accumulates when oxygen is lacking [15]. This factor activates signaling pathways which regulate proliferation and death. It was often observed that HiF-1α accumulates in cancer cells [16,17]. This accumulation can be due to the physiological chronic hypoxia of cancer cells or genetically determined, for instance by the mutations affecting the Von Hippel-Lindau (VHL) gene [18].
However, the study of the effects of hypoxia using HeLa-Fucci cell line is complex since the lack of oxygen was shown to modify the fluorescence of Kusabira Orange and Azami Green, as shown by Kaida et al., [19,20]. This artefact makes difficult the use of HeLa-Fucci cells in real hypoxic conditions. As a consequence, the study of the effect of the hypoxia pathway, notably the stabilization of HiF-1α, on cell cycle dynamics using this cell line could be done through the use of hypoxia-mimetic chemicals. Indeed, various molecules can be used to mimic hypoxia by inducing HiF-1α accumulation, such as Cobalt Chloride (CoCl2), Dimethyloxalylglycine (DMOG), Desferrioxamine (DFO). These molecules are also studied for their anti-cancer effect, as iron-chelators [21,22]. In a general way, they induce HiF-1α accumulation, but they also have numerous HiF-1α-independent effects [23]. Notably, they are known to induce apoptosis [8] and cell cycle arrest through complex interactions with regulating proteins [22]. CoCl2 and DFO are certainly the most commonly used hypoxia-mimetic molecules [24]. As a consequence, their biological effects were relatively well tested over the last years. They both induce apoptosis when their concentration is high enough [8,25,26]. However, CoCl2 seems to promote cell survival during the first 6-8 hours of treatment [27,28]. Cell proliferation in presence of these agents was assessed using different cell lines. Dai et al., (2012) [25] have shown that in presence of CoCl2, PC-2 cells growth was stimulated during 72h. Then, a dose-dependent inhibition of growth was observed, with an increase of the cell death rate. In a general way, CoCl2 and DFO are known to inhibit cell proliferation [24]. However, their precise influence on cell cycle is variable. In presence of CoCl2, some studies report an arrest in the G1 phase [24,29], or in the G2 phase [30]. In the case of DFO, it is not clear if DFO blocks the cell cycle in G1, S, or G2 phase. Many studies report an arrest on G1 phase or in S phase, depending on dose and cell type [24,31-33]. A recent paper by Siriwardana et al., [34] could precisely differentiate a mid-G1 phase arrest and a S phase arrest. This result suggests that these two types of cell cycle blockage act more or less in presence of DFO, depending on cell line. In the other hand, some authors also noticed an arrest during the G2 phase [22,35]. Clearly, the effects of DFO and CoCl2 on cell cycle dynamics closely depend on the dose used, the time of exposure, and the cell line studied.
As a consequence, in order to use these molecules as HiF-1α inducers or anti-cancer agents on HeLa-Fucci cells, a preliminary study of their biological effects on this cell line is required. Notably, it is crucial to characterize their effect on the cell cycle, using the fluorescent properties of the Fucci cells. It would also bring additional knowledge on the biological response to these anti-cancer molecules. This work thus proposes, for the first time, to investigate the effects of iron-chelators DFO and CoCl2 on HeLa-Fucci cells proliferation dynamics. We constructed our study as follows. We first verified that a lack of oxygen generates fluorescence extinction, even in moderate hypoxia (3% O2). To evaluate the toxicity of DFO and CoCl2, we studied their influence on cell death and cell growth of HeLa-Fucci cells. This first step is necessary to rigorously interpret the fluorescent data. It also provides interesting comparative results of the cellular effects of the two molecules. Then, we studied their actions on the cell cycle dynamics of HeLa-Fucci cells. We show that this cell line exhibits an original response to iron-chelators DFO and CoCl2, quite different from observations obtained with other cell lines, which have mainly shown an arrest at the G1/S transition [22]. In our experimental conditions, DFO generates a G2 phase arrest of the cell cycle when its concentration passes over a threshold. CoCl2 acts on cell proliferation following a complex biphasic effect. Depending on dose and time of exposure, it promotes or inhibits the entry into the cycle. By using some known elements of the genetic characteristics of HeLa cells, we propose a molecular mechanism to explain our observations. It gives a hypothesis to test the action of DFO and CoCl2 on the MAPK pathway.
Culture conditions
For the experiments in hypoxia we used a SANYO MCO-5M cell culture incubator which allows one to impose various conditions of oxygen pressure on the cells.
Cell death measurements
Fluorescent imaging
Cell cycle phases definition
Figure 1: Fluorescent images of HeLa-Fucci cells.
B. Example of a microscopic field with Fucci-cells in different phases of the cycle.
Image processing and statistics
Fluorescence extinction of HeLa-Fucci cells in hypoxic conditions
B. The red and C. the green fluorescence signals after reoxygenation are plotted as functions of time. The values are calculated by considering the average of the 10% most fluorescent pixels of each field, and normalized to the maximal value, as done by Kaida et al., [18]. The statistics are calculated using student's test, with respect to the fluorescence measured 20min after reoxygenation.
Estimation of DFO and CoCl2 toxicity
DFO promotes cell death and inhibits cell growth
B. Growth profile of HeLa-Fucci cells treated with 100/500 μM of DFO. Statistics were calculated using Student's test, with respect to the control group.
Dose and time dependency of CoCl2 effect on cell death and growth
Figure 4: Effect of CoCl2 on cell growth and mortality.
B. Growth profile of HeLa-Fucci cells treated with 100/200 μM CoCl2. Statistics were calculated using Student's test, with respect to the control group.
DFO promotes cell cycle arrest in G2 phase of HeLa-Fucci cells
B. Representative field of cells on a cover glass cultured during 24h with 500 μM DFO.
CoCl2 has a biphasic effect on HeLa-Fucci cells proliferation dynamics
In this work, we tested for the first time, on HeLa-Fucci cells, the effects on cell death, cell growth and cell cycle of two widely used hypoxia-mimetic molecules (DFO and CoCl2). Our principal result concerns the characterization of the cell cycle dynamics using fluorescent microscopy. We found that CoCl2 has a biphasic effect on this cell line. It first promotes the progression into the G0/G1 phase, and inhibits it in a second time. DFO was found to induce cell cycle arrest in the G2 phase, following a switch-like behavior. When its concentration reaches a threshold (10 μM), more than 80% of the cells stop in this phase with a kinetics depending on DFO concentration. This observation leads to various types of conclusions. First, it provides interesting information about the use of hypoxia-mimetic agents on Fucci cells. We notably found that the effects of the molecules used are different from the known cellular response of hypoxia, which tends to promote cell cycle arrest in the G1 phase [9,37,38]. We note here that the use of HeLa-Fucci cells allowed us to distinguish the G0 from a G1init phase, which would not have been possible with other techniques such as flow cytometry. We could thus precisely quantify the effects of CoCl2 on the entry into the cycle. From our work, it appears that DFO and CoCl2 certainly have a large range of biological effects on HeLa-Fucci cells which hides the consequences of HiF-1α induction. This conclusion shows strong obstacles to the use of HeLa-Fucci cells to study the effect of hypoxia on the cell cycle. However, since we have shown that the fluorescence recovers after about 2-3 hours in normoxia, this cell line can be a good tool to study the influence of reoxygenation on the cell cycle dynamics. The tools we developed in this study could be used towards this interesting perspective.
Second, the cellular response to iron-depletion has been intensely studied over these last years [22]. In this framework, the results we obtained with the HeLa-Fucci cell line are surprising. Whereas these two molecules were often shown to promote cell cycle arrest in the G1 or S phases, they act differently in the HeLa-Fucci cell line. The biphasic effect of CoCl2 on cell growth was reported in the study by Dai et al., [25], but the authors did not explain this observation by precisely studying the cell cycle dynamics. As the Fucci cells allow us to follow the G0/G1 progression (non-colored to red cells), we could observe the biphasic effect of CoCl2 on the entry into the cycle. Moreover, the G2 phase arrest in presence of DFO was rarely observed [22]. How can we explain the particular features of the response of HeLa-Fucci to iron-chelators DFO and CoCl2? In their review, Yu et al., [22] note that one of the principal actor of the G1/S arrest in presence of these molecules is the p53 protein, which inhibits the cyclins D and E. Yet, HeLa cells are known to have low p53 activity [39-41]. This could explain the absence of a G1 phase arrest. This idea is confirmed by the fact that the cell lines exhibiting a G1 phase arrest in presence of DFO or CoCl2 seem to present a wild-type p53 activity: hMSC [24], UCC-2 [29], T-Lymphocytes [32]. Interestingly, the study by Brodie et al., [31] quantified the proliferation of two cell lines in presence of DFO. The SKN-SH cell line (neuroblastoma) is very sensitive to the G1 phase arrest. It has a non-mutated p53 activity, as suggested by the results of Kim et al., [42]. On the contrary, the T98G cell line (glioblastoma) is not sensitive to the G1 phase arrest, and it presents a low p53 activity [43]. The low p53 activity of HeLa cells is also coherent with the high resistance to apoptosis we observed in this study. If p53 cannot explain the cell response to DFO and CoCl2, which pathway could be involved?
The MAPK signaling cascade is strongly activated by iron-chelators [22]. More specifically, it was shown that DFO and CoCl2 activate this pathway [30,44-46]. Besides, it is functional in the HeLa cell line [47,48]. The enzymes of the MAPK family, notably p38, ERK and MEK have numerous effects on the cell cycle. First, they are known to inhibit the G2/M transition, and to promote the G2 phase arrest of the cell cycle [49]. Second, it was shown that they have an effect on the G0/G1 progression. On the one hand, ERK1/2 can inhibit the progression into the G0/G1 phase by promoting the synthesis of Mirk/Dyrk1B [50]. On the second hand, it was recently shown that in HeLa cells, ERK1/2 promotes proliferation by activating the progression into the G1 phase [51]. Thus, ERK can have two opposite effects on cell proliferation. It provides a promising candidate (or mechanism?) to explain the biphasic response to CoCl2 we observed with HeLa-Fucci cells. Interestingly, a biphasic effect related to MAPK activation by Cd and Hg was observed by Hao et al., [52]. As a consequence, our hypothesis that the response of HeLa-Fucci cells to CoCl2 treatment is due to the activation of the MAPK pathway may be relevant. Following this view, in presence of CoCl2, proteins activating cell proliferation are primarily synthesized. The work of Bai et al., [51] suggests that the p-c-fos protein could be involved in this step. Then, Mirk/Dyrk1B gene is expressed, and the progression into the G0/G1 phase is slowed down. As our experiments show that the biphasic time-response to CoCl2 is coupled to a biphasic dose-response, we can suggest that the differential activation of the Mirk and p-c-fos pathways could also depend on the concentration of the chemical. However, in this framework, it remains difficult to understand why DFO and CoCl2 have different actions on the cell cycle dynamics. It may depend on the type of enzymes activated by each of these agents. DFO would activate the MAPK-mediated G2 phase arrest and CoCl2 the biphasic action of MAPK on the G0/G1 progression. The complexity of the MAPK pathway and the huge number of its targets do not allow us to propose a more precise hypothesis. In all cases, the idea of a predominance of the MAPK pathway, because of the inactivity of p53, is an interesting hypothesis. It could be tested with molecular biology protocols. There would be many approaches to test. That is why we chose to limit our work to a first investigation at the cellular scale. For instance, it would be interesting to observe the effects of chemical inhibitions of the MAPK signaling cascade, as made in previous studies [49,51]. If our hypothesis is verified, HeLa-Fucci cells would be a good tool to precisely study the poorly known impact of iron-chelators agents on the MAPK pathway.
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Citation:Bedessem B, Marie-Paule M, Hamel M, Giroud F, Stéphanou A (2015) Effects of the Hypoxia-Mimetic Agents DFO and CoCl2 on HeLa-Fucci Cells. J Cell Biol Cell Metab 2: 004.
Copyright: © 2015 Baptiste Bedessem, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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OCA2 (tyrosinase-positive albinism) is the most common form of generalized albinism characterized at birth by the presence of a certain amount of pigment in the skin and in the eyes of patients who continue to produce it and accumulate it throughout life. The skin is white, does not show general pigmentation and does not tan with exposure to the sun.
As for OCA1, there is a possibility for the development of pigmented lesions of the skin (freckles and lentigo), often on sun-exposed areas, but usually does not result in a tan.
The hair can be very weakly pigmented, taking on a yellow or light blonde, or with more pigments, strong blonde, golden blonde or even red.
The iris is blue-grey, sometimes slightly pigmented, the degree of transparency, visible with transillumination, depends on the amount of pigment present.
OCA2 is the product of a mutation of the human P gene, which was mapped on chromosome 15q11-12 that codes for the protein P responsible of the tyrosine's protein across the membrane of the melanosome. Tyrosine activity is normal.
Go back to oculocutaneous albinism
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Permissions beyond the scope of this license may be available at firstname.lastname@example.com.


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$50,000 Powerball Winner Sold in Edgewood
Sat, 12/28/2019 - 14:43 RCN Newsdesk
One lucky Kentucky Lottery player in Edgewood scored a $50,000 winner in the Christmas Day Powerball drawing Wednesday.
According to Kentucky Lottery officials, the winning ticket was sold at Thorntons store #553 on Madison Avenue.
No winner was publicly identified yet.
The store will receive a bonus for selling a winning ticket.
$50,000 is awarded to a ticket-holder who correctly selected four of five white balls and the Powerball.
Sunday's Powerball drawing jackpot is estimated at $200 million.
-Staff report


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Barely Gettin' By season three: American Carnage
Podcast hosts Dr Emma Shortis and Dr Chloe Ward are back for season three and as they continue examining the inner workings of American democracy, their eyes are trained on the US election.
How can we better prepare suburban Melbourne for climate change?
Climate change presents critical challenges for future development, sustainability and resilience across Melbourne. Here, our experts offer insights on how we can better prepare our outer suburbs.
New map shows Melbourne's parks will struggle in next stage of lockdown
Looking forward to catching up with friends in a park as lockdown eases? Unfortunately, so is everyone else – and we're going to need more space, finds new RMIT research.
Ten ways RMIT research is helping to build a more sustainable future
From turning back the emissions clock to building more durable roads from old tyres, RMIT researchers are tackling today's biggest challenges and developing solutions for a more sustainable world.


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Only results with
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Minutes : Victoria U.F.A. and U.F.W.A. District Political Association
Constitution of the Lethbridge District UF.A. Association
Constitution of the Vegreville U.F.A. Federal Constituency Association
Paul Soldan certificate of appreciation
Two Hills card lock and petroleum agency
Tank Painting
Cardston farm supply store assessment
Two Hills Farm Supply Store assessment and construction
Letter outlining the history of the U.F.A. and explaining the relationship between the U.F.A. and the United Grain Growers
Maple Leaf Petroleum Ltd. mortgages
Farm Store Flyers and Flat Sheets
Farm Supply Flyers, Flat Sheets, Ad Mats, Memoranda and Petroleum brochure
Farm Supply Flyers, Flat Sheets and Memoranda
Farm Store Advertisements and Flyers
Farm Store Flat Sheets and Ad Mats
UFA Heavy Horse Hitch at Remington Carriage Museum, Cardston
Cardston Site Profile
Two Hills Site Profile
Two Hills Farm Supply
Marshall Draganiuk
Peter Keryluk
Lynn Sommerfeldt
Eli Antoniuk
George Stringam
Two Hills Advisory Council Meeting
Driver Sheldon Ouiatt, Cardston Agency
Two Hills Farm Store
Cardston - Nielson
Two Hills - Foerster
Two Hills - Dmytriw
Two Hills opening
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Published: Feb 6, 2020, 7:03 am • Updated: Feb 6, 2020, 7:04 am By Associated Press
By STEVE LeBLANC
BOSTON (AP) — Massachusetts House lawmakers approved a bill Wednesday that would tighten oversight of payments made by marijuana companies to the cities and towns that host them.
The bill, which passed by a 121-33 vote, would give the state's Cannabis Control Commission the power to review, regulate and enforce "host community agreements" following criticism that some local officials have used the contracts to demand more money from companies than is allowed under state law.
Read the rest of this story on BostonHerald.com.
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2SPIM131 Game Engine Architecture
Learning outcomes
By completing the course, the student will have achieved the following learning outcomes:
The student
- has knowledge of the architecture and parts of a 3D game engine
- has knowledge of rendering optimisation
The student
- is able to use tools and methods for programming large, complex systems
- is able to find, evaluate, use and refer to relevant research and development work and other relevant academic material
- is fully conversant with critical thinking, logic, communication and problem solving
General competence
The student
- is able to disseminate key academic material orally and in writing, take part in professional discussions within the subject area and share their knowledge and experiences with others
Course content
Central topics:
- 3D rendering optimisation
- 3D sound
- materials
- scene graphs/scene organisation
- light/shade
- collision detection
- physics simulation
- types of animation
- particle systems
- input
- scripting
Teaching and working methods
The course is organised as a combination of lectures, practical exercises, self-study and supervision.
Coursework requirements
- 2-4 individual assignments
- attendance at lectures in accordance with the teaching plan
- attendance at laboratory teaching in accordance with the teaching plan
- 4-hour individual written examination which counts for 50% of the final grade
- 1 individual assignment which counts for 50% of the final grade
To pass the course, both examinations must receive a passing grade.
Alphabetical grades are used, on a scale from A to F, with E as the lowest passing grade.


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Our guest is Dave Westerveld. We talk a bit about accessibility, challenges in testing LMS software, and a lot of "modern" topics.
Follow him on twitter @offbeattesting, and his blog at https://offbeattesting.com/
Podcast: Play in new window | Download
Subscribe: Apple Podcasts | Android | Email | Google Podcasts | RSS | More


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Search Status
The Search Status component
Working with Search Criteria
Search criteria may be added by using any of the available search methods, filtering the current view.
Individual search criteria may be removed with the clear icon on each term, or all terms may be cleared by clicking the Home button.
Search criteria clear buttons
Home button
Types of Criteria
Each type of search will display slightly different criteria:
Quick Search
Search Filter
NOT Search Filter
Advanced Search
Selected Directory
Folder Transparency and Opacity
When a folder is selected in the Directory Browser, MediaBeacon's default behavior is to display all assets in that folder and all subfolders in the current View component, this is referred to as folder transparency.
In order to filter for those assets located in the root of a folder, the user can click a "directory" search criteria (after first clicking a folder in the Directory Browser). The search criteria is then put in "folder opacity" mode, indicated by an opaque folder icon in the left-hand side of the directory search. In this mode, subfolder's contents are not displayed in the View component.
Advanced Operators
Advanced operators (AND, OR), also known as boolean operators, are used to combine search criteria in advanced types of searching. The option to view criteria with these operators is configured by an administrator.
When displayed, these operators can be clicked to toggle between "AND" and "OR".
For more detail on how to use advanced operators, see the [Search Filter] section.
Editable Search Criteria
In MediaBeacon 2019.2 and above, the values of search criteria may now be directly edited. The user may click on a search criteria's text, and it will enter an edit mode, allowing the user to substitute a string.
- Advanced Search criteria values, before and after
- Search Filter criteria values, before and after


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About me and my eToro Journey
My name is Joseph Moricca. I grew up in a small village in Northamptonshire, England. I started my first job when I was 13 helping my uncles on construction sites and continued working while learning throughout my academic life. At school, I ran tuck shops out of my locker and used that money to start trading. Initially, I tried to day trade but found the constant price swings and concentration required very mentally draining. I knew I had to develop a strategy that could still earn a market-beating return but also allowed me to work the job I love.
As a result, I have been trading and learning about trading for the last seven years. I opened my eToro account while at University and during my free time developed my strategy. While learning and engaging in trading, I still received a First-Class Honours in Quantity Surveying (Equivalent to a 4.0 GPA)!
My Day Job
As a Quantity Surveyor, my main duties are initial project feasibility, cost management, and trying to create cost savings for clients. As a Quantity Surveyor, I have worked on various construction projects ranging from £500k to £45 million in value. However, while I l love working on various construction projects, I wanted to do something separate from my primary career that still could benefit me.
As a result, I became a Popular Investor on eToro. Part of my growth strategy is to self-trade on higher risk/reward stocks. By doing this, I help diversify my portfolio and can encourage greater personal growth. Initially, I started trading at 16 on various crypto exchanges, but more recently I have switched to a mid-term to long term trading strategy that perfectly suits my lifestyle and has started to earn me a second income.
My Personal Ethos
I believe that at the core of any good investor or financial strategy is a strong ethos. The purpose of my trading on eToro is to accelerate my growth through a higher risk diversification strategy.
I believe that a consistent and sustained effort will yield results. If you do decide to copy me on eToro, I will do my best to share my knowledge. If you have not read it, you should check out my FREE beginners guide to trading on eToro for hints and tips into beginning with eToro!
Alternatively, if you are ready, you can join eToro below!
My Personal Trading
I am a popular investor on eToro and earn a salary based primarily on AUM (assets under management). Essential through the eToro platform, anyone can copy my trading strategy, by investing anywhere between $200-$500,000, for free! The way this works is that I get paid by eToro who charges a small fee for each newly opened trade position (known as a spread).
I also make a secondary revenue stream by operating as and an eToro partner. As an eToro partner, I make a commission for people signing up, depositing and using eToro through one of my links. Copying anyone on eToro does NOT cost you anything and does not affect the price for you and just because you go through my website does not mean you have to invest with me or anyone else!
My Trading History on eToro
Please note my personal trading is separate from The Passive Trading System!
Initially, in 2017, I tried a highly aggressive trading strategy which failed, but as with anyone who starts on a new platform, this was only a small loss.
I then spent almost a year reading and studying about investing, reading around 50 books, 100s of websites and taking various courses to develop my skills. In 2018 I made a -27% loss and I spent the year changing my strategy and developing my approach to trading to reduce my risk and increase profitability.
In 2019, I started the third iteration of my strategy and have not looked back. In 2019 I currently have a return of over 70%! I still tweak my strategy to increase my profitability and to reduce my risk. Luckily for any new investor, I have done the hard work and taken the risk of developing the strategy, so now it is time to tweak the method and to grow my wealth!
I am now confident in my strategy! And if you wish to copy me you are more than welcome to! However, I think my list of eToro top traders may provide better alternatives! Either way I want to invite new investors to join eToro and grow your wealth just as I have. Even if you are not yet convinced by my stats and style I still suggest signing up to eToro and getting a feel of the platform they have a fantastic demo mode so that you can trial the platform without losing any money! Also if your not sure where to get started check out my beginners guide to trading on eToro for hints and tricks!


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After posting an all-time low in February, New Home Sales rebounded strongly last month.
Based on joint research from the Census Bureau and HUD, 300,000 new, single-family homes were sold on a seasonally-adjusted, annualized basis in March. It's an 11 percent improvement from February, and right in-line with the 6-month average.
The supply of available new homes improved, too, in March, falling by close to a full month.
At the current pace of sales, the entire new home housing stock would be sold in 7.3 months. This is the second-best reading in a year, a statistic partially-supported by the relatively small number of new homes on the market.
There are now just 183,000 new homes available for sale across Orland Park and the country. That's the smallest reading since the Census Bureau started to keep New Home Sales records beginning in 1995.
However, it should be noted that the March New Home Sales data is suspect. The reading's margin of error exceeds it actual measurement by almost double. It's possible that sales volume fell in March instead of rising, therefore. The Census Bureau says as much in its footnotes:
The change [in new home sales] is not statistically significant; that is, it is uncertain whether there was an increase or decrease [in March 2011].
We won't know for certain until future data revisions are made.
If you're a home buyer in Beverly , though, and want to stay ahead of the market, you won't want to take chances. If the Census Bureau finds its data to be accurate after revisions are made, new home prices will already have started to rise.
You may get your best home value by buying sooner rather than later.


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kayeight wrote:what's up, I'm 24/F/Colorado and I've been lurking here for a while. I'm a software developer and I like dogs and eating candy.
iamacyborg wrote:As a fellow Suit Supply MtM purchaser, I have no idea how you can pull 3 blog posts worth of content out of it :/


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Submit search
In Stock - Usually ships in 1-3 (M-F) days - Guaranteed Same Day Shipping for Orders with UPS 1, 2 or 3-days shipping method selected (not USPS)
As seen in the mind-boggling Trans-Siberian stage show! This thought-provoking medley of Christmas tunes describes a lone cellist playing Christmas carols in the midst of the Bosnian war. The piece reflects the power, drama, and hope of a single musician in the midst of war, reflecting on the power of music to bring hope. The rock rhythm section contributes to an intense and very hip musical experience. Optional parts for electric guitar or electric violin can be used to add interest to the performance. This arrangement includes parts appropriate for a community or professional orchestra. The bowings are those that would be expected by professional players. Instrumentation: Full orchestra, timpani (B-E), drumset, opt. electric guitar, opt. electric violin, opt. piano, opt. electric bass. (3:10)
View a Sample Page . . . Sample Page 1 . . .


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As part of October Fire Prevention month, VVSD and the Bolingbrook Fire Department team up for a student art contest regarding fire safety and prevention.
The winners of the contest get a ride to school in a fire truck.
Salk Elementary School second grade students in Pam Dzik's and Erica Thomas' class wait for the arrival of Teresa Lira, this year's contest winner from Salk.


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Searches should be conducted by you on a few of the most popular social media websites like facebook, Instagram Twitter YouTube, and Pinterest. It'd be highly unlikely to meet someone in this day and age who does not possess at least one of those social networking accounts.
Locate a reason. I take her hand and spin her around to check her ass out. This is obviously sending a sign to her telling her I find her attractive but it is likewise make her comfortable with us touching that is important.
Typically, when you go on a job interview you speak about yourself, share your best qualities and try to convince the person interviewing you thatyou're the very best man for your job, right? Well, on composing a profile, or a date, it is virtually the exact same thing.
Once again, It is likely that the partnership will break down if you do not share a lot of the core points that are necessary to every of you. If you don't share them, you must at the very least regard the other person's worths, and also understand that individuals often alter what they value. . . yet only when it is their choice.
She feels the very same insecurities and anxieties that we all face and is human just like you. You simply don't notice becauseyou're too wrapped up in your own emotions. Unfortunately there is an epidemic of insecure women out there grappling with everything from problems of self worth, desirability, backpage escorts image, aging, and the stability of the relationships to name a few.
It will be better, since you won't be crying in the bathroom stall in Boston Pizza since you just saw your boy- rating based dating apps nuzzle the ear of the girl from high school who was able to scream, " Kaylee's East Advocate NS real backpage escorts is showing! " Through Phys Ed.
I was forced to stare into the abyss of" single" reality. I had been totally unprepared for this particular predicament. The chance had never entered my stream of consciousness, and I felt like I drowned in a sea of East Advocate free casual sex webisites and bewilderment, without a lifeline to save myself. How was I going to live this psychological catastrophe? I had been to discover that to get a union relationship to thrive there are as I sought to understand the mechanisms of what had happened. By way of instance, the couple should keep living the romantic pictures of falling into love if facing the realities of the life changes caused as time passes.
What I didn't realise at the point is that waiting is the worst thing you can perform. I would begin to overthink and become really negative" The girl isn't interested" , " She's likely talking to other guys" etc. .
Write those ideas that are negative down and work on them. Don't just accept them for what they are. Analyze them and tear them apart piece by piece and replace them with ideas. Anytime you catch it, catch yourself in a notion that is poor and replace it with something greater. This won't be easy and won't happen overnight.
Also, when it comes to compliments, obviously you can give them if East Advocate Nova Scotia casual sex ad good be, but quit overdoing things to constantly make a woman happy with everything you say and do. If you continue placing up women on a pedestal then guess what- - they will always be looking DOWN on you personally, and you may be looking UP in them.
Here is a question that must have been at the forefront of your new backpage escorts Taymouth while you read this cons section: " how do I know whether my matches are whom they say they're? " Becauseyou're chatting with someone over written word, i. e. before you progressively hookers and cocaine meme East Advocate Nova Scotia the conversation into a more personal style of communication, it's mostly impossible to determine if what the individual on the other end of the digital conversation is he or she says they're. For example, when looking at how online dating swindles work, we saw that the internet makes it possible for high school pupil, Adebukola out of Nigeria, to pose as John Smith out of Idaho and utilize this misrepresentation to prey unknowing, thirsty for romance singles( mind you, this is an extreme instance) .
Going Out For Your First Time So much was written about picking up girls, and my information is part of a chorus that was rather large. However I do have casual sex ladies porn East Advocate Nova Scotia bit of information to help out newbies that is not as commonly repeated. We are going to discuss approach anxiety but the idea is to engage somebody in a conversation in a way that feels natural. The more you fear about stating some thing, the more likely you'll mess up.
But remember, you are not taking crap anymore, and that means you are sifting through them to get a guy who is capable of respecting you. And he's in there. As a fantastic guy, who's in a good place in his life, is attracted to a woman who is also in a place that was fantastic.
I am not saying you should never buy girls items. Ifyou're going on a java date if you are in a relationship with a girl for a while or buy her the coffee, you can buy her some jewellery. But you shouldn't spend hundreds of bucks on your first date. Unless you want to entice gold- diggers stop trying to prove yourself using cash.
Yes, this has happened to me and I dare say that it has happened to many other online daters. However, it has not dissuaded me and that I hope you won't allow you to discourage. You keep moving forward and free thai dating apps East Advocate your site.
But this isn't real. If your companion wants to go, they will. A lover is not a non- living things- - you can not' hangon' to them- - they are people with free backpage escorts tips East Advocate NS and also dreams and also suitables of their very own. What you can do is realize that each person seeks their own joy. In some cases it may be with you, at other times it might not. And also if you still look after he or she, the very best you can do is permit them to follow their dreams. Gracefully step apart as well as want them well. The right partner will go along for you one day.
***Sexual communication begins and ends with all the sexual backpage escorts hiring Goose Cove NL at the present love those hookers tee, reflected in your body. The second you try to induce it, or look away and they disconnect and jump back into your head, is precisely the moment in which you lose the connection. That feeling must be communicated first then, and through your entire body through your words. If you do not, when she does not make the move to you you'll be wondering what could have been. Do this slowly, mindful of the other person's bounds, and then continue to let nature take its course as far as you actively enjoy it.
" What? " I countered. " It's the human condition! " So long as we, and as long as we walk this planet, we'll make errors make errors, Christ's blood will pay for us. God asks us to do our best keep our hearts soft to His leading and to follow Him.
In what looked like an instant, it was finished and that I was left to ponder what had happened. All the questions that were normal swarmed throughout my mind. Where had I gone wrong, who was to blame, and was there any chance of salvaging the relationship? I began to peel away the layers of ignorance and delusion and blindsight as I agonized through the past and present. I began to observe the what, where and the when of the corrosion of my connection.
I was in the library also, you know, the period which you truly spend studying is lower than the time spent on your mobile phone, when you study next to you. I shot my smartphone and began texting. I texted her" Hey, what are you wearing today? " She sent me a photo of her dress, and I got crazy there; I told her that we had to meet for a coffee during lunch break, and I specified that we had to meet in the front of the elevator to go to the library. I pressed the button to go from the basement, although we took the elevator when we met. We did it in there.
They stayed with Todd she had within the physical borders of their security agreements. About how she and Todd had agreed to share info with each 21, she did not communicate to Liam. ( I talked about information sharing in the" Inquire Within" segment) The unfortunate, and avoidable, fallout of this was while they chatted online shortly following the date Liam had with Juliet that Liam shared data. Liam supposed they had a" Firehose Method" method of sharing details and advised Todd of information he wasn't prepared to listen to about the date( like sexual interactions) . This wasn't in compliance with the arrangements Todd and Juliet had in place. Liam meant no harm and believed it was okay to escorts backpage that will rob you East Advocate NS because they had been in an open connection. However, this led to Todd being unpleasantly surprised by the information and needing to set very clear boundaries about how, when, and who he wishes to convey with regarding spouse interactions.
In a universe with more than seven billion people, rejection only means next! " Knowledge is not power until it is applied. " - - Dale Carnegie Parting Thoughts Recall, online dating is one of many methods to meet someone new, find a mate, or date. It's much too easy to rely on one method of East Advocate NS top escorts backpage with new folks if you opt to become idle. Happy hours, East Advocate different networking events, or going out dancing, can cause a delay in meeting someone.
Let us start our exploration of the" hot" topic by recognizing that anger is a East Advocate NS lexy bandera fuck buddy( an emotion) , not a behavior, and it is one all of us experience, though every one of us reacts in our own manner. Anger events have social, psychological, and physical components although you may not be aware of them. Your heartbeat and breathing rate up, your muscles tense, your thoughts concentrate on" what( or who) went wrong, " and you will express your angry feelings in words or activities that involve other men and women.
My cousin met a woman on JDate who lived two blocks from his property. What good luck for both of these, since neither you wanted to sail! They have since sold both of their first homes and purchased together a beautiful home in the same neighborhood.


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SpaceX test-fired and Falcon 9 rocket at Cape Canaveral Friday evening, igniting nine Merlin engines for several seconds while hold-down restraints kept the launcher on the ground. (19659003) The instantaneous launch opportunity Wednesday is set for 6:24 pm EDT (2224 GMT), roughly the moment Earth's rotation brings Cape Canaveral under the orbital plane of the International Space Station
A Dragon cargo capsule previously flown on two resupply missions to the station in April 2015 and December 2017 will ride into orbit on top of the Falcon 9 launcher. If the launch and rendezvous go according to plan, the automated supply carrier will arrive at the space station early Friday with several tons of cargo, food and experiments.
It's the first time that SpaceX has flown a Dragon Capsule three times. Hans Koenigsmann, SpaceX's vice president of construction and flight reliability, said earlier this year that the first-generation Dragon Spacecraft – set for retirement next year – is capable of flying to three missions in space.
18th launch to resupply the space station under a $ 3.04 billion freight transport contract with NASA. First signed in 2008, the deal covers 20 resupply missions, and SpaceX also holds separate multibillion-dollar NASA contracts for additional supply deliveries in the early 2020s, and for the development of the Crew Dragon Capsule designed to carry both cargo and astronauts [19659003] SpaceX will return the Falcon 9 launcher to its hangar at pad 40 over the weekend for attachment of the Dragon cargo craft.
The Falcon 9's first stage will return to Cape Canaveral for a racket-assisted touchdown at Landing Zone 1 around eight minutes after liftoff Wednesday evening.
The hold-down firing occurred at 6 pm EDT (2200 GMT) Friday after SpaceX worked through technical issues that delayed the test from earlier in the week. SpaceX originally planned to conduct the static fire test Tuesday, and the Falcon 9 launch team loaded the rocket with propellants before apparently aborting the mock countdown.
The troubles that delayed the static fire test also forced SpaceX to push back the mission's launch from Sunday to Wednesday.
Items drained for delivery to the station inside the Dragon's pressurized cabin include a 3D BioFabrication Facility developed by Techshot, an Indiana-based company that demonstrates printing soft tissue in microgravity, and capability researchers view as a stepping stone
A habitat carrying 40 female mice will also launch within SpaceX's Dragon spacecraft. The capsula will return to Earth with 30 of the mice to Earth after approximately one month in orbit, and specimens from the mice will be distributed to medical and biologists to investigate how spaceflight affected the animals
The other 10 mice will remain on
The Dragon will also deliver a new docking adapter that will be used by SpaceX and Boeing commercial crew capsules to link up with the space station
The International Docking Adapter-3, or IDA- 3, was built by Boeing to replace a unit lost during a SpaceX launch failure in 2015. SpaceX successfully delivered IDA-2 to the station in 2016, and the new docking mechanism was first used in March by SpaceX's Crew Dragon Spacecraft on an unpiloted test
The robotic arm's space station will pull IDA-3 out of the Dragon's cargo capsule's rear payload bay and place it on the space-facing zenith port of the Harmony module's space station, allowing Follow Stephen Clark on Twitter : @ StephenClark1 .


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Tuesday, 25 August 2009
The cross-linking of suitable formulations by base-catalysed polymerisation and polycondensation reactions is well known for use in conventional coating applications and is highly attractive forcertain applications. Transferring the experiences from coatings, Benno Blickenstorfer reports on the design and the application of photolatent bases into adhesives formulations, as this technologyis able to offer considerable economic and ecological advantages.
By Kirsten Wrede


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ceClub: Synthesizing Concurrent Relational Data Structures
- Speaker:
- Roman Manevich (UT Austin)
- Date:
- Wednesday, 8.2.2012, 11:30
- Place:
- EE Meyer Building 861
Efficient concurrent data structures are extremely important for obtaining good performance for most parallel programs. However, ensuring the correctness of concurrent data structure implementations can be very tricky because of concurrency bugs such as race conditions and deadlocks. In systems that use optimistic parallel execution such as boosted transactional memory systems and the Galois system, the implementation of concurrent data structures is even more complex because data structure implementations must also detect conflicts between concurrent activities and support the rollback of conflicting activities.
At present, these types of concurrent data structures are implemented manually by expert programmers who write explicitly parallel code packaged into libraries for use by application programmers. This solution has its limitations; for example, it does not permit the customization or tuning of a data structure implementation for a particular application.
In this talk, we present Autograph, which is the first concurrent data structure compiler that can synthesize concurrent relational data structure implementations for use by application programmers. The input to Autograph is a high-level declarative specification of an abstract data type (ADT); the output is a concurrent implementation of that ADT with conflict detection and rollback baked in. Our synthesizer is parameterized by a set of data structures called "tiles", which are building blocks that the compiler composes to create the overall data structure. Application programmers can use a simple expression language to tune the composition of these tiles, thereby exercising high level, fine-grain control of data structure implementations. We have used Autograph to synthesize concurrent sparse graph data structures for a number of complex parallel graph benchmarks. Our results show that the synthesized concurrent data structures usually perform better than the handwritten ones; for some applications and thread counts, they improve performance by a factor of 2.


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Wylie's Smith Library has a host of activities planned for February -- and they're all free! Come in out of the cold and see what's going on ...
Bandan Koro - Theatre & History - Feb. 6, 6:30 p.m.
Bandan Koro African Drum and Dance Ensemble returns with a new program, presenting theatrical selections of traditionally based West African drumming and dance, along with presentations of traditional folklore. For adults and families; best suited to kids third grade and older. Register at WylieTexas.gov/library.
3D Design with Tinkercad - Feb. 7, 6:30 p.m.
Learn the basics of 3D design using Tinkercad. Participants will create a chess piece suitable for printing on the library's 3D printer. No 3D experience necessary, but must be familiar with using a mouse and keyboard. For adults 18 and older. Class size limited; register at WylieTexas.gov/library. Create a free Tinkercad account at www.tinkercad.com prior to the workshop.
Lego League – Feb. 6 & 8, 3:30 to 4:30 p.m.
Kids in grades 1 - 4 invited to show off creativity and build around a different theme each month. Cooperation and building on theme encouraged but not required. Age limits strictly enforced. No parents or younger/older siblings, please. Space is limited; register at the Children's Desk. February's theme: What Will I Be?
My Very Own Book Club – Feb. 13 & 15, 3:30 to 4:30 p.m.
Kids in grades 1 and 2 encouraged to read the book of the month on their own time and then come to the meeting for discussion and a craft. Sign up at the Children's Desk. Participation is limited; all participants get a free copy of the book. February's title: "Wonder Horse: The Story of the World's Smartest Horse," by Emily Arnold McCully.
Teen Anti-Valentine's Day Party - Feb. 14, 6 to 7 p.m.
Kids in grades 6 - 12, come celebrate all things anti-Valentine's with chocolate, games, Mad-Libs, and other crafts.
3rd Grade Voyagers Club – Feb. 21, 3:30 to 4:30 p.m.
Third-graders invited to this learning club just for you! Each month a new topic is explored. Register at the Children's Desk to receive your challenge. Space is limited. February's topic: Yoga with Ms. Christy.
BeTween Book & Movie Club – Feb. 28, 6 to 7 p.m.
Kids in grades 4, 5 and 6 invited to discuss books that have movie adaptations. Read the book and watch movie on your own, then come to the meeting to watch scenes from the movie, read excerpts from the book, discuss and have a snack. Sign up at the Children's Desk; first 50 kids get a free copy of the book. February's title: "The Little Prince," by Antoine de Saint-Exupery.
Teen Book Club – Feb. 7, 6 to 7 p.m.
Sixth- through 12th-graders invited to share snacks and drinks and discuss the book of the month. Stop by the Teen Room for more information and to register. February's title: "The Unlikely Hero of Room 13B," by Teresa Toten.
Teen/Tween Poetry Café - Feb. 18, 5 to 7 p.m.
Aspiring poets, sign up for library's poetry café, presented by the SPL Teen Library Council. The audience will spend the evening listening to Wylie's young poets, complete with refreshments and Parisian atmosphere. Not a contest; a showcase of young talent. Kids in grades 4 - 12 invited to sign up in Teen Room now through Feb. 9 for a performance slot. Submit chosen poems to email@example.com for approval after registering. Mandatory rehearsal on Feb. 16 between 3 and 5 p.m.
Japanimation Club – Feb. 21, 6 to 7 p.m.
Sixth-through 12th-graders, come geek out about all things anime and manga in the Multi-Purpose Room. No registration required.
Family Lego Build – Feb. 27, 3:30 to 4:45 p.m.
Come show off your creativity by building with the library's Lego and Duplo sets in the Storytime Room. All ages welcome; no registration required. Children age 9 and younger must be accompanied by adult.
Booked on Fridays – Feb. 8, noon
Adults 18 and up invited to bring lunch or snack, enjoy good reads and conversation. Meets the second Friday of the month. Copies of the featured book available at the Help Desk or on Overdrive. February's title: "Barracoon: The Story of the Last 'Black Cargo'," by Zora Neale Hurston.
Hygge at the Library - Feb. 28, 6 p.m.
Hygge (pronounced hoo-ga) is the Danish word used to acknowledge a moment as cozy or special. Unwind and unplug from the everyday rush and practice lots of hygge in library's cozy space with tea and self-directed crafts. Register at WylieTexas.gov/library.


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AAPA: Senate Bill Cuts Key Port Grant Program
On Thursday, the American Association of Port Authorities (AAPA) voiced concern about the U.S. Senate's approval of a FY2020 appropriations bill that would cut funding for the Maritime Administration's Port Infrastructure Development Program by about $200 million. The program provides grants for port infrastructure projects through a competitive application process.
MARAD's Port Infrastructure Development Program was initiated last year with a first round of funding of $290 million, made possible in part by the support of Congressman Mario Díaz-Balart (R-FL). The program will provide 15 major coastal seaports and a selection of others with financial support for projects that deploy advanced technology, enhance resiliency or facilitate agricultural exports, among other goals. The application process for the first round closed in September.
If the Senate's appropriation mark is upheld in the final FY2020 bill, the program would be cut to $90 million next year. The Senate bill also requires that deep-draft coastal ports and shallow-draft inland waterways ports must share the funds - a change from the conditions for the FY2019 round, which was restricted to coastal seaports with at least 20 feet of water depth. For FY2020, AAPA is calling for funding the program at a minimum level of $300 million for deep-draft coastal ports only.
The House of Representatives approved $225 million for the program in its version of the FY2020 appropriations bill. AAPA says that it will fight to secure a higher funding level for the program in the final conference negotiations.
"It's critical that the U.S. invest in its port infrastructure to address growing trade, which is the lifeblood of our economy," said Chris Connor, AAPA's president and CEO. "MARAD's Port Infrastructure Development Program is the first and only federal grant program dedicated to our nation's ports, and direct investment into port-related infrastructure is a top AAPA priority. Seaports are a crucial part of our nation's transportation infrastructure and serve as vital freight gateways to the global marketplace for our military and for American farmers, manufacturers, retailers and consumers."


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#201 40 Lakeshore Drive, Ne, NE Salmon Arm, Corona Building, V1E4M9
- 2,550 SqFt
Salmon Arm, British Columbia
Great Downtown Core Location For This Second Floor Office Space. 2,550 Square Foot Space Consists Of Spacious Reception Area, Several Offices, Work Stations, Meeting And Storage Rooms. Interactive 360 Degree Virtual Tour Available At Www.jg ...(more)


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Special Prices for Star Wars Checks from Checks Unlimited
April 2, 2016 Uncategorized No Comments
If you're old enough to remember the original Star Wars movies then you're going to love Star Wars checks from Checks Unlimited! We know that not many people use checks these days but if you're one of them, this is a great way to show your love for Star Wars!
There are Star Wars checks from four episodes: A New Hope, The Empire Strikes Back, Return of the Jedi and even The Force Awakens checks. These are not intended to use for business, they are personal checks.
Special offer: If you're a new customer you can pay as little as $6.50 a box plus get a 4th box free. There are also Star Wars check covers, labels and credit card holders.
Checks come with:
- Security Features: including Chemical Protection, Erasure Protection and Microprint Signature Line.
- Duplicate Checks: Save time and keep better records with carbonless copies of every check you write.
- Images enhanced to show detail on the check.
Here's a preview of the Star Wars checks from Checks Unlimited:
Featuring personal checks with your favorite Star Wars characters:
•Princess Leia
•C-3PO and R2-D2
•Darth Vader and Obi-Wan Kenobi
•C-3PO and R2-D2 walking together
•Darth Vader, Emperor Palpatine with stormtroopers
•Han Solo trapped in carbonite
These are a favorite!
Plus, matching checkbook covers.
Let your geekiness show with these Star Wars checks.


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Otaru Villa Moun Teng
Otaru Villa Moun Teng is located in Otaru and takes about 12 minutes by bus from JR Otaru Station, near Otaru Park and Otaru Tenguyama Ski Park. Free WiFi is available in the lobby. Yukatas are available for guests to wear during their stay. Facilities include a coin laundry. The hotel possesses 8 guest rooms.
|Room Type
ROH Room
Shared Bathroom
Up to 5 people
Highly Recommended! Breakfast Plan
Breakfast:included / Dinner:none
Pay at hotel
per night per person
Standard Single Room
Shared Bathroom
Up to 1 people
Must-See Offer! Breakfast Plan
Breakfast:included / Dinner:none
Pay at hotel
per night per person
Hotel Facilities
- non-smoking rooms
- vending machine
- coin laundry (pay to use)
- ski wear rental
- snowboard rental
- ski drying room
Room Facilities
- Internet (WiFi, in some rooms)
- electric kettle
- tea set
- refrigerator
- hair dryer
- body soap
- 2-in-1 shampoo and conditioner
- toothbrush
- towels
- yukata (Japanese-style robe)
Available Services
- computer access available
- express delivery
Closest station:
Phone number:
Fax number:
Please note
If you are late for check-in time, please call the hotel directly.There is no closing time.
Extra bed is not available.
Cancellation policy
Should you cancel your booking, a cancellation fee will be charged to you according to the date (Japan Standard Time) the booking changes and cancellation request are accepted.*Special cancellation policies may apply to certain dates and accommodation plans.Please confirm the cancellation policy on the booking step pages before completing your reservation.
- No-show
- 100%
- On the date of arrival
- 100%
- One day prior to arrival
- 50%
- 3 days prior to arrival
- 30%
Places to eat
- Breakfast
- dining hall
- Dinner
- dining hall
- Bath (Type):family bath
- sea-bathing
- fishing
- cruising
- tennis
- soccer
- skiing
- cross country skiing
- aquarium
- foraging for wild edible plants


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Special Library Programs & Events
View our EVENTS CALENDAR for a complete list of Library Programs
or view our monthly newsletter, Happenings @ Your Library »
Libraries closed temporarily. No fines during this time. Stay safe.
1,000 Books Before Kindergarten
Reading with your baby, toddler or preschooler is one of the best ways to prepare your child for success in school. Starting November 1, parents can sign up their children for the program – it's never too early or too late to start. The 1,000 Books program is open to all children from birth until they start kindergarten.
The logo and name "1,000 Books Before Kindergarten" are used with permission from the 1,000 Books Foundation. https://1000booksbeforekindergarten.org/


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BMW International Open Golf Preview
If you're wading through past renewals of this contest, then you need to take note that this year's venue Golf Club Gut Larchenoff hosted the 2012 and 2014 renewals of this events, and not the years in between.
Meanwhile, the other big consideration is whether you think that the players who played in those monstrously tough conditions in the US Open last week will be too knackered to give it their all here, or will they be relieved to get back to a golf course with a natural degree of difficulty.
That quandary especially applies to Danny Willett and Henrik Stenson, who are the two form horses in the field. Personally, whilst the 10/1 about either of them is fair value, I think they'll struggle to be at their very best this week – and have decided to look for bigger value elsewhere.
Tyrell Hatton 1 point each-way @ 33/1 (Various Bookies) ¼ odds 5 places
The fiery young Englishman is top of my list this week, as he could well be the next English Player to take a big step forward once he wins his first European Tour event. His 22nd here in 2014 was a fair performance on his first look at the track, and recent form figures of 5/7/20 suggest he's in the sort of form that should see him go close.
Fabrizio Zanotti 1 point each-way @ 80/1 (Betfred, Stan James) ¼ odds 5 places
The 2014 Champion looks to be a decent price this week considering he also finished 12th here in 2012. Add in the fact that he's finished in the top-10 in 2 of his last 4 events, and it's not hard to see him being around the top of the leaderboard come weekend.
Nicholas Colsaerts 1 point each-way @ 50/1 (Coral) ¼ odds 5 places
The big-hitting self-confessed playboy seems to be aiming to get his game back together this year, and he's certainly got plenty of game having previously made the Ryder Cup Team. In his last 5 events he's finished 15/3/23/22/3, and he could use his power to great advantage around here.
Kiradech Aphibarnrat 1 point each-way @ 100/1 (Coral) ¼ odds 5 places
The power angle also applies to Aphibarnrat who, whilst blowing extremely hot and cold, can mix it with the best on the European Tour when in the mood. He's pretty cold at the moment, but did hit form by finishing fourth in the event last year, be it on a different track.
Brandon Stone 1 point each-way @ 80/1 (Various Bookies) ¼ odds 5 places
Another player with fond memories of this event is Brandon Stone, who finished tenth at one of the other venues on an invite a few years ago. It's took him a while to make good on the promise of that event – but he's another who hits it miles and could be ready to step-up to win this prestigious event.


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With the NBA trade deadline approaching, there are teams that should be very active on the trade market. The Kristaps Porzingis trade has shocked NBA fans, but more are sure to come. These teams are all competing for a playoff spot, but need another push to be taken seriously. All of these teams are being reported to have interest in making a move before the NBA trade deadline. In this article, I break down what players they should be looking for and what affect trades could have on these teams. Charlotte Hornets As the Hornets remain on the edge of the playoffs, they look to add pieces to bolster their chances. The biggest move they're trying to make is a Marc Gasol
Tag: mike conley
Trade Finder: Memphis Grizzlies
The Memphis Grizzlies need a trade. Reports recently came out that the Memphis Grizzlies are open to trading their franchise cornerstones, Marc Gasol and Mike Conley. This may be overdue, as the grit and grind style of Memphis is ineffective in today's NBA. Luckily, the team can build around Jaren Jackson and finally look towards the future. As the grit and grind era comes to an end in Memphis, I looked for what trades can be made to jumpstart the Grizzlies rebuild. Trade #1 Grizzlies Recieve: Reggie Jackson, Jon Leuer, Stanley Johnson, 2019 1st Round Pick (Top 10 Protected), 2019 + 2020 Second Round Pick Pistons Recieve: Mike Conley, Omri Casspi For the Grizzlies, they manage to recoup decent value for their aging star on
Can Mike Conley lead the Grizzlies to the Playoffs?
After missing all of last season, Mike Conley is back on the court. Without him, Memphis struggled, winning just 22 games. Last season contained the lowest win total in the last 10 years for the Grizzlies. Now that he's back, the Grizzlies are back to their competitive ways. Between the readdition of Conley and the drafting of the rookie Jaren Jackson Jr, the Grizzlies have found themselves atop the challenging West. Jackson has been so good that he landed #3 in my rookie power rankings, but that's not what this article is about. The focus is on Conley and how he's turned this team around. How has Mike Conley Made the Grizzlies Good? Mike Conley has easily been the Grizzlies' best player.


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BJP rides the 'lion' for elections
The BJP on Friday tweeted a combination video of clips of two speeches of Narendra Modi, one from 1992 and the other from 2019, with a caption proclaiming that "the attitude of lions doesn't change", underlining the party's effort to turn the upcoming general election into a referendum on the leader.
The BJP is striving to re-ignite a 2014-like wave in favour of Modi by over-stressing a perceived threat to national security if the country returns to the coalition era led by fractious Opposition parties.
The top leadership believes that turning the Lok Sabha polls into a referendum on Modi, riding on the narrative of a "strong leader", is the only way to secure a 2014-like majority on its own.
The first clip of Modi's speech, dated January 24, 1992, is from the "Ekta Yatra" led by then party chief Murli Manohar Joshi to unfurl the Tricolour at Lal Chowk in Srinagar on Republic Day.
Modi, who had been managing the yatra, is seen at a public meeting daring the terrorists, who had threatened the BJP against hoisting the national flag at Lal Chowk.
"On January 26, the day after tomorrow, it will be decided at Lal Chowk who has drunk his mother's milk," Modi says in the short clip, while pointing out that terrorists had put up posters at Lal Chowk threatening to kill anyone who dares to unfurl the national flag.
In the second clip, dated March 4, 2019, in the aftermath of the February 14 terror strike in Kashmir's Pulwama, Modi says at a rally in Bihar: "The fire that is burning in your heart, countrymen, the same fire burns in my heart too. Even if they are under the earth, they will not be spared. I can't wait long. It is my nature to settle scores."
A tweet in Hindi along with the video said: "The attitude of lions doesn't change."
The intention was clear — to project Modi as a daredevil and strong leader when it comes to national security.
The BJP strategy, according to party managers, is to unceasingly try to make Modi the cornerstone of the elections to shift attention from anti-incumbency over the perceived economic failures of the government, the farm distress and joblessness.
"It will be only Modi and Modi in the polls. We will turn this into a presidential-style election," a BJP general secretary said. "We will overwhelm the voters with the idea that Modi is the only alternative before the country," he added.
Following the Pulwama terror attack and particularly after the retaliatory air strikes in Pakistan's Balakot targeting Jaish-e-Mohammed camps, the BJP has been steadfastly trying to keep the issue alive, hoping the anti-Pakistan/terror bogie would generate a Modi wave before the polls.
Fearing that the euphoria among the masses over the air strikes might dissipate by the time the votes are cast, the BJP is leaving no stone unturned to keep the issue alive.
BJP leaders, led by finance minister Arun Jaitley and party chief Amit Shah, are writing blogs, holding media conferences and posting on Twitter. Party MPs and leaders have been asked to make use of every opportunity to paint the Congress pro-Pakistan with a dilly-dallying attitude towards terrorism.
"Apart from talking about the various welfare schemes, the constant focus during the campaign would be on the tough stand taken by the Modi government against Pakistan-sponsored terrorists," a BJP MP said.
The MP felt that the Congress was falling into the BJP's trap by engaging on terror and the Chinese veto on the UN designating Jaish chief Masood Azhar as a global terrorist.
While keeping the Pakistan/terrorism pot boiling, the focus is on repeatedly stressing that Modi is the only "strong and decisive" leader that the country needs. BJP leaders believe that doing so would sway the masses and turn the tide in the party's favour when the votes are polled in April-May.


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Fans are confused by La La Anthony's latest photo because it seems that she has morphed into the talented singer and songwriter Keri Hilson.
The Power actress has ditched the long blond hair for some sexy dreadlocks and used a cute filter to debut the new look.
In the photo, Carmelo Anthony's wife is wearing a camouflage sweater while sitting in a car.
The Two Can Play That Game and You Got Served star used the caption to explain that she was on a road trip.
As stated above, fans are forced to take a double look because La La is twinning with Keri.
One fan had this to tell the mother of one: "I honestly thought this was Keri Hilson lol. Is Lala still with Carmelo, or did they go their separate ways, completely forgot when the NBA season started, but I got time now. Lala can do whatever she's gone always be popping. This is how I wanna do my hair for the summer. But these crazy filters are doing whole plastic surgeries on faces….we're going to get a whole wave of a generation with facial dysmorphia because of these damn filters."
Another commenter shared: "You and Keri Hilson are beginning to look like twins, beautiful pic. She can wear anything she has style. This filter got everybody looking the same. Lol. You were at my nieces' McDonald's in Pennsville Nj you showed her Love too!!! She was soo hype. She looks different, still beautiful tho."
A third supporter stated: "She's beautiful any style fits her. She's gorgeous too, just as pretty as Keri Hilson. Look it here what a surprise where have my La La been let me guess behind the scenes well it's nice to see a face of a familiar generation so I'm glad your well and looking good I hope more of you are out there. How can a guy cheat on this Beautiful Masterpiece GOD made @lala LORD if you are reading this, allow me to blessed with a woman similar to her."
In several recent interviews, La La confessed that she is talking to Carmelo and a reconciliation is possible after she filed for divorce over cheating allegations.
A tipster told Hollywood Life: "La La still loves Carmelo, he's her one true love, and she can't imagine life without him. But, he really hurt her, he broke her trust and her heart, so she's not going to take him back until she's made him truly realize what he almost lost."
What are your thoughts on the new hair?