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Press release • Jul 06, 2018 19:35 PST
The Reality
Virtual Reality (VR) and Augmented Reality (AR) have been buzzwords for quite some time. The idea that you can strap on a VR headset, however clumsy it may appear, and totally immerse yourself in a virtual world makes great headlines and appeals to tech fans and gamers everywhere.
Augmented Reality is quite a different proposition. While VR has tended to dominate the news, AR has been quietly breaking new ground in the enterprise sector.
Where VR lets users escape into a totally different reality, AR is proving highly effective in our own world, as it overlays digital imagery onto the reality we are experiencing. When used with wearable technology such as smart glasses, this blending of digital information with the physical world allows hands-free operation in such environments as healthcare, logistics, maintenance, and construction, where the ability to devote both hands to the specific task is critical. Operators can benefit from remote work assistance and projected instruction manuals, saving time, increasing productivity and enhancing safety.
Adoption of Augmented Reality
The market opportunity for Augmented Reality is clearly not just for gamers or consumers. It directly targets the enterprise market of technical and skilled workers, from engineers to architects to healthcare professionals.
The potential for the technology is perhaps best underlined by the fact that major tech industry players are committed to developing AR marketing. Apple, Microsoft, Google, and Facebook all are providing deep toolsets for developers to create apps for this approach. All have quietly adopted the implicit assumption that a persistent, wearable artificial reality is the next big thing.[1]
Indeed, as AR adoption gains momentum with an increasing number of industrial applications being launched, some businesses are expected to soon place smart glasses at the core of their IoT systems, as they look to enhance worker productivity and streamline their backend operations.[2]
According to Amy Kwa, Regional Manager, Visual Products, "Deploying AR will enable more efficient processes by enhancing the reality of the user, so they'll be able for example to maintain an engine or a complex electrical board in an intuitive and easy way. They'll be able to see inside the device and act on the information there and then."
Epson launched their first Moverio smart glasses model in Southeast Asia in 2012 and have continued to expand their product range. Their latest models, the BT-350 and BT-2200, offer video and access to new AR experiences for a variety of commercial and industrial market applications respectively.
Augmented Reality through Smart Glasses
These include healthcare, where Moverio smart glasses are helping surgeons and clinicians concentrate fully on their patients by freeing them from manually handling data, allowing them to focus on their often complex tasks. Dentists, too, find their work is greatly aided by Moverio, using the smart glasses to provide a precise heads-up overlay of their patient's teeth. This enables better hand-eye coordination and a more precise treatment time.
As smart glasses evolve to become a truly seamless experience that users interact with as a daily routine, one of the most important issues for developers of AR wearables to address is form factor. Future generations of glasses are expected to offer wifi, stereo 3D graphics and enhanced processing of images and audio.
The user's choice of smart glasses will depend on their purpose. Key considerations include their ability to deliver digital information crisply and legibly, but also on their weight and comfort.
For use in applications such as healthcare or at museums, galleries and tourist destinations where they can provide background data on whatever the visitor is viewing, the components must be packaged into a lightweight and compact format. Operational management is also a consideration for such environments, where a multiple-device management dock or admin software could be ideal.
But when operators are using the glasses in a heavy industrial job site that may also be hot and cramped, it is crucial that they are not a distraction. The glasses need to be in a headset format secured with a firm headband, or even adjustable to fit over a safety helmet, for workplaces where these are mandatory.
Smart glasses, of course, do not have prescription lenses, so it is important that they can fit comfortably over the wearer's normal glasses in every environment.
It is likely that adoption of AR technology will reach a tipping point this year. Developers will be launching innovative new apps to grow the commercial and consumer markets, and working to overcome issues with smart glasses such as predictive head motion tracking to reduce 'motion to photon' latency, as well as the constraints on power and thermal factors necessary to keep the glasses cool.
As smart glasses become more common, will we perhaps see them being worn by style leaders as sleek fashion accessories? Whether smart glasses appear on the catwalks of Paris and Milan or not, the potential for AR combined with wearables is huge. Following the personal computer, the Internet and the smartphone, AR and smart glasses are likely to be the world's next transformative technology.
[1] https://www.wired.com/story/future-of-augmented-reality-2018/
[2] http://www.eurekamagazine.co.uk/design-engineering-features/technology/augmented-reality-is-increasingly-finding-implementation-among-workforces/167020/#.WlTMxT9k1kY
About Epson
Epson is a global technology leader dedicated to connecting people, things and information with its original efficient, compact and precision technologies. With a lineup that ranges from inkjet printers and digital printing systems to 3LCD projectors, watches and industrial robots, the company is focused on driving innovations and exceeding customer expectations in inkjet, visual communications, wearables and robotics.
Led by the Japan-based Seiko Epson Corporation, the Epson Group comprises more than 76,000 employees in 87 companies around the world, and is proud of its contributions to the communities in which it operates and its ongoing efforts to reduce environmental impacts.
About Epson Singapore
Since 1982, Epson has developed a strong presence across major markets in Southeast Asia and South Asia. Led by the regional headquarters Epson Singapore, Epson's business in Southeast Asia spans an extensive network of 11 countries with a comprehensive infrastructure of close to 500 service outlets, 7 Epson solution centres and 7 manufacturing facilities.
About Epson Philippines
Epson's corporate presence in the Philippines commenced in 1998. It grew rapidly into a billion-peso company in a span of a few years from its establishment due to its client-oriented approach in its business management style by its dedicated and innovative staff members and officers. Epson Philippines has an extensive network of 77 Authorized Service Centers nationwide with more than 200 dealers and more than 800 shops across the Country. For more details, please visit our website: www.epson.com.ph or our Facebook page: http://www.facebook.com/EpsonPhilippines
For product inquiries and technical support, please contact Epson Customer Care at: Metro Manila: (02) 441-9030; Toll Free: 1-800-1069-37766 (PLDT), 1-800-3-0037766 (DIGITEL) or visit the Epson Customer Care website: http://customercare.epson.com.ph


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Showing 29–41 of 41 results
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Showing 29–41 of 41 results

subset: commoncrawl


text:
Join in this opportunity now.
We need event staff to help out at the Amaechi Basketball Centre before and after Manchester Magic home games, which take place on Saturdays at 6.30pm. Before the game, the court needs to be swept and cleaned, the bleachers need to be pulled out, benches need to be put in place, tables for scorers and statisticians need to be erected and court side banners need to be put up. After the game, the bleachers need to be cleared of rubbish and put away, the court needs to be swept, benches, tables and banners need to be put away and rubbish bins need to be emptied.
Placement offered by Greater Manchester Community Basketball
Contact – Phil Gordos
Phone – 07872412926
Perks and volunteer incentives
Free tea or coffee. Free volunteer t-shirt. Free entry to the game.
Please do not reply to this email.


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Songs literally keep Dave Stewart up at night.
"It drives me mad," said the former Eurythmics guitarist-songwriter-producer, who made beautiful music with singer Annie Lennox starting in the '80s.
"I know Michael Jackson had the same problem of melodies waking him up all the time and a lot of people do – songwriters. And often I've found I've written on the bed sheet instead of on the notebook. And my wife is like, 'What the hell? Not again! Ink!'"
Nocturnal difficulties aside, it should come as no surprise then that Stewart is one of the creative forces behind the new show 'Songland', debuting Tuesday on NBC and Citytv.
"The show is probably the opposite to most of those (music competition TV) shows," said Stewart, 66, who's also worked with Mick Jagger, Paul McCartney, Ringo Starr, U2's Bono and The Edge, Tom Petty, and Stevie Nicks.
"I thought (back in 2012), 'Listen, I am going to have to create a show that allows songwriters to come on and sing their own songs.' A lot of (the process), the general public has not really understood yet, and this is the first time we see the curtain drawn back and you actually see creativity happening in real time."
We caught up with Stewart in Toronto recently at the Ritz Carlton's Deq Lounge.
Q. What are the components of Songland?
A. The (three) panelists – (OneRepublic's) Ryan Tedder, urban-pop songwriter Ester Dean and country music's Shane McAnally) – (are) brilliant at producing and songwriting too. And then the fourth person is our guest artist – Aloe Blacc or John Legend or the Jonas Brothers or Kelsea Ballerini – the list goes on. And the (artists) are going to choose (three out of four songwriters and ultimately) one of the songs and record it and it's going to come out that exact same night that the show airs.
Q. Are you ever going to be on the show?
A. I'm in the middle of writing three musicals. One is called The Time Traveler's Wife and Lauren Gunderson has rewritten it to a play and now I'm writing the songs with Joss Stone. And I'm writing one that is very autobiographical and the director (Lorne Campbell), who I'm writing the book working with, he actually just rewrote and directed Sting's musical, The Last Ship, that was just (in Toronto). It's kind of based on the fact that I come from this town, like the Billy Elliot character, I wanted to be a footballer, and I had my left knee sort of broken in half when I was about 13. My brother brought in an old Spanish guitar off my grandmother's wall that had three strings and a lot of dust on it and I was not listening to music at all. I've written six or seven of the songs already. And I've been writing with an amazing lady from Detroit something called Here Comes The Rain, but it's not really something to do with the Eurythmics or anything. It's to do with a tiny pocket of New York that is having problems with the police.
Q. How is Annie?
A. She's not somebody that wants to go on tour or anything like that. I mean, you get to a certain point in your life and you've got to choose wisely what you do. But she's very active. She's always been like this. Anything that didn't seem right or fair and particularly in the women's movement. But as far as the Eurythmics touring as the Eurythmics, she will never tour as Eurythmics again. It's too demanding. I'm more of a nutcase, well not really a nutcase, but it's not as demanding for me as it is for the lead singer. So I'll probably be playing and touring until I'm 70-odd or whatever, and writing songs and musicals.
Q. How is Mick Jagger doing after his heart surgery?
A. He's evidently a lot better. Mick and I use text a lot. And Mick's a man of few words like, 'Doing alright.' But he's one of these people that's so lean and fit. I've spent so many long hours, days and weeks with him writing songs. There was one time, we were in his place in Mustique, and he has a room that's a cross between a gym but it had a nice echo-y sound so we were recording in there, but while I was busy working out something, he'd just do a 120 press-ups. And then we were doing something else he'd do 300 sit-ups. And then running six miles (9.6 km).
Q. He sounds superhuman.
A. He was laughing, actually, when they did "Oldchella' (a.k.a. Desert Trip on the Coachella grounds in 2016). He said, 'Bloody hell, Dave, the stage was like even longer than a Stones stage.' And he says, looking at the other people on the bill, whether it Bob Dylan (or Neil Young, etc.), 'I don't think they're going to be using all of that stage. I wonder why they built that stage that big?'


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Download free Doctor Who PC games
November 9, 2010
I am a fan of Doctor Who. It is sci fi, so no real surprise there.
The BBC is giving away PC games featuring the latest Doctor and his assistant Amy Pond.
The games are pretty good fun and can be downloaded for free here.


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Assurance report on the sustainability information in the BASF Report 2011¹ ²
To the Management of BASF SE
Introduction and scope
We have been engaged to perform a limited assurance engagement on sustainability performance information included in the BASF Report 2011" for the business year 2011 of BASF SE, Ludwigshafen. The "BASF Report 2011" contains the Management's Analysis, which is, together with the consolidated financial statements for the year ended December 31, 2011 subject to the statutory group audit.
Sustainability performance information included in the scope of our assurance is within the following sections of the "BASF Report 2011": About this report, To our shareholder (Letter from the Chairman of the Board of Executive Directors, BASF on the capital market), The BASF Group (Strategy and values, Our goals, Sustainability management, Value added statement), The business year at BASF, Employees and society (Employees, Occupational safety and health protection, Social commitment) Environment and safety (Environmental, safety and security management, Climate protection, Energy and raw materials, Water, Product stewardship), Corporate Governance of the BASF Group (Compliance, Management and Supervisory Boards, compensation report), and Overviews (Requirements, topics and data, GRI and Global Compact Index).
Management's responsibility
Management is responsible for the appropriateness of the preparation and presentation of qualitative claims and the appropriateness of the determination and presentation of quantitative indicators on sustainability performance included in the "BASF Report 2011" in accordance with the Sustainability Reporting Guidelines (G3.1) of the Global Reporting Initiative as well as internal guidelines, as described in the sections "About this report" (page 4) and "Requirements, topics and data" (page 224).
This responsibility includes designing, implementing and maintaining systems and processes relevant for the preparation of the sustainability performance information, as well as the selection of topics and the application of appropriate methods for the preparation of the report.
Auditor's responsibility and assurance standards
Our responsibility is to carry out an assurance engagement on the sustainability performance information in the "BASF Report 2011" and to express a conclusion with limited assurance based on the assurance procedures performed.
We conducted our engagement in accordance with the International Standard for Assurance Engagements (ISAE) 3000:"Assurance Engagements other than Audits or Reviews of Historical Financial Information", issued by the International Auditing and Assurance Standards Board. Amongst others, this standard requires that the members of our assurance team have appropriate knowledge, skills and professional qualifications to understand and evaluate the sustainability performance information, and that we comply with the requirements of IFAC of the Code of Ethics for Professional Accountants to ensure our independence.
According to ISAE we have to plan and perform the assurance engagement such that we are able to express our conclusion as to whether any matters have come to our attention that cause us to believe that the sustainability performance information in the "BASF Report 2011" is not, in all material respects, fairly stated.
In a limited assurance engagement, the scope evidence gathering procedures is less comprehensive than in a reasonable assurance engagement, and therefore less assurance is obtained than in a reasonable assurance engagement.
This assurance engagement is limited primarily to inquiries of employees, especially those responsible for the preparation and presentation of qualitative claims and the determination and the presentation quantitative indicators on sustainability performance information in the "BASF Report 2011, analytical and other evidence gathering procedures, as appropriate.
In particular we carried out the following assurance procedures:
- Interviews of employees on Group level in order to gain an understanding of the process for determining material sustainability topics of BASF SE.
- A media analysis and internet search for references to sustainability performance information of BASF SE in the reporting period.
- Inspection of the documentation of the systems, processes and internal controls for information management as well as for processing data on Group and Regional level.
- Enquiries of employees responsible for the analysis and reporting of the sustainability performance indicators.
- Assessment of the data as reported and of data validation processes on Group and Regional level and analytical procedures regarding data trends.
- Site visits in Lampertheim and Schwarzheide (both Germany), Shanghai Caojing and Shanghai Pudong (both China) and Tarragona (Spain) to assess local data collection and reporting processes and the reliability of the reported data.
- Enquiries of employees on Group level responsible for the preparation of the sections Sustainability management, Strategies and values, Our goals, Employees and society, Environment and safety, Compliance and Overviews.
- Inspections of internal and external documents and sources, in particular related to the topics Stakeholder engagement, Our corporate carbon footprint, Opportunities with climate protection products, Management of new technologies, The challenge of demographic change, Supply chain management, Occupational safety focus areas and Transportation safety, to determine whether qualitative claims are based on sufficient evidence and presented in an accurate and balanced manner.
- An evaluation of the overall presentation of the sustainability performance within the scope of our engagement.
Based on the procedures performed nothing has come to our attention that causes us to believe that the sustainability performance information in the "BASF Report 2011" is not, in all material respects, fairly stated.
Frankfurt am Main, February 22, 2012
[Original German version signed by:]
1 Translation of the independent assurance report, authoritative in German language.
2 Our engagement applies to the German Version of the BASF Report 2011.


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One of the hardest things at CopyCamp for most creators will be to get past thinking that the "them" they are fighting against are large transnational corporations. In my personal experience most of the debates are not of the form "us vs them", but really "us vs. us", meaning it is disagreements (sometimes quite strong) between fellow authors/creators that are at the heart of most copyright issues.
I was reminded of this when reading the Session List on the WIKI [3], and the entry submitted by Andre Cornellier under the title of "How to turn all this talk into action".
To quote:
It is great to have ideas about how to solve all the problems of copyright, but if nobody puts them into law what is the use? We all want to get the government to address the problem of the copyright law, but we do not have the money to work with major lobbying firms.
It has taken more then 8 years, after the last copyright change, in 1998, for the government to address artists' concerns. Then an election came and the whole agenda was wasted. A big lobby comes along, the education ministers lobby, and they get priority.
How can we, creators, make the government move on our demands, our needs? And this before the next century is over……How to give our needs clout, and momentum?
This write-up assumes that creators are a unified group who agree, and that we can come together to move on our collective demands.
I've met Andre a number of times in the past. We met at the 2003 "Ministers forum on Copyright", at various Creators' Rights Alliance events, and on opposite sides of the S-9 debates (photography [4]) in the senate.
I hear his name often mentioned in the so-called "educational use of the Internet" debate in support of the Access Copyright proposal. This is often in the context of the confusion that some creators have between the appropriate use of TPMs that are needed to indicate licensing choices on the Internet, and the entirely different abuse of TPMs that have often come under the title of DRM (Digital Restrictions Management).
The three issues that are of most concern to me as a creator are:
- the legalization and legal protection of the abuse of TPMs that take basic rights away from the owners of information technology (DRM, Broadcast Flags, attempts to close the "Analog hole"), as well as circumvent many other laws such as anti-trust/competition, privacy, etc.
- the imposition of business models by the government on creators, removing the rights of the author to explore a full spectrum of methods of production, distribution and funding (Example: extended licensing or exemptions for educational use of the Internet)
- the expansion or obfuscation of the term of copyright (Example: The proposed change to have life of photographer added to the term of photographs, even though the photographer is unknown/unknowable for the vast majority of photographs)
On all three of these issues I have been on the opposite side of the table from Andre. I don't know if I qualify as being part of a "big lobby", given I'm entirely self-financed and self-motivated for all the volunteer lobby work I am currently doing.
Will it be possible for the two of us to sit down and chat about these issues so we can come to an understanding? Will any attempt we make to discuss these issues only end in unhealthy arguments? Are there going to be enough people at CopyCamp who can act as bridges between creators with such strongly opposing opinions?
While I believe there is a need for governments to protect the rights of creators, we as creators need to realize that we don't yet agree on what changes would help or hinder our rights. We first need to come to an agreement as creators before we try to "blame" the government for not "moving forward" on proposals that many creators believe are against the interests of creators.


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Tucked away from the bustles of the city yet close to all urban facilities, WINDSOR CASTLE, the prestigious residential project from QUEENS HABITATS Builders has all the comforts of modern living. Spread over 20 floors, this magnificent tower houses 74 aesthetically designed apartments comprising of 1Bhk,2Bhk, 3Bhk and 4 Bhk units suiting every individual requirements. WINDSOR CASTLE, coming up in the beautiful landscape of Paropady just 100 meters from NH-212 is ideally located near to reputed educational institutions, hospitals and shopping places. Like every other projects of QUEENS, WINDSOR CASTLE also endorses the concept of peaceful life and graceful living. QUEENS HABITATS, one of the most trusted builders in Calicut, have completed several projects including villas and apartments. Upholding the tradition of trust, QUEENS has always excelled in quality, transparency, timely completion, after sales service and all other aspects of home building.
Children's Play Area
Indoor Games
Landscaped Gardens
Power Backup
Swimming Pool
Queens habitats, the renowned builder in Calicut, provides quality apartments and villas to the discerning home seekers. With an unshakable reputation in ethical practices and timely delivery, Queens offers homes in the most premium locations in Kozhikode. Having experience for over a decade in building flats and villas, we have delivered hundreds of homes to our satisfied customers. The quality of our homes is at par with the best in the country and Our Quality Policy vouches on the same. Queens Habitats has eminent personalities on its Director Board vouching for its trust and acceptance. The vision and zeal of these directors are reflected on every project that we undertake.
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Katy Perry: My relationship with Orlando Bloom is only 'part of me'
Katy Perry has opened up about wanting to keep details of her relationship with Orlando Bloom to a minimum so it doesn't become the main topic of her life.
The 'Part of Me' hitmaker has admitted she doesn't mind speaking about her partner, but says it's just "one part" of her and she wouldn't want the romance to overtake other aspects of her life such as her religion and art.
Referring to meeting Pope Francis with the 'Pirates of the Caribbean' actor - whom she rekindled her romance with after a year apart - and her mother Mary Hudson in April, Katy told Vogue Australia: "It's OK to mention him.
"I don't want it to be a headline of the story, because it takes away from the purpose.
"Also, it's extremely misogynistic. Of course, I love my relationship, but that is one part of me, and I don't want any part of what I do to be diminished."
However, the 33-year-old pop star - who went through a very public break up with ex-husband Russell Brand in 2012 - accepts that people are bound to talk about her relationships because of her status.
She added: "There will always be noise."
During their trip to Rome, Italy, the 'Dark Horse' singer referred to Orlando as her "darling" when she revealed she was there to give a talk on meditation at the Vatican's fourth annual Unite to Cure conference.
Taking to her Instagram stories, she said at the time: "Happy Saturday everyone, I'm here in Rome at the Vatican, to speak on meditation. I'm with my momma, my darling [Orlando], and my manager, Steve! It's a great day! I am so excited!"
Orlando, 41, admitted recently that their romance came as a "complete surprise" to him but says they have a "remarkable connection".
He said: "It is a very odd one. She's a remarkable human being, actually. Completely surprising, because I am not her demographic. I am a little older than her, so I wasn't someone overly familiar with her artistic endeavours when we met. I wouldn't have imagined anything happening. And, you know, when you get hit sideways you get hit sideways.
"You don't pick who you fall in love with. And then you come around and there's all sorts of things that happen in between, right? We had a really remarkable connection. And it's difficult. She's on tour, I'm doing a play, everything evolves. I have a lot of respect for her and a lot of deep feeling. So we will see."
Katy Perry is currently performing in Johannesburg, South Africa, as part of her 'Witness' world tour. Her first show on 18 July, followed by performances on 19 and 20 July.


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Learn more about who we are as well as contact information, bell schedules, and more...
Information on HSE's Non-Discrimination and Non-Harassment Policies.
HSE21 is the teaching and learning framework in HSE schools. Learn more about how HSE21 is preparing our students for the future.
HSE utilizes a 1:1 model of technology integration. Learn more about how digital devices in our schools foster collaboration, creativity, communication, and critical thinking.
Meet with your Academic Adviser to prepare for next year's courses...
Our counseling team has two licensed school counselors who will be available to you throughout your time at our school.
Keep updated on daily events by reading our school's announcements...
Keep up with current events with our weekly newsletter.
With school starting soon, visit our site to learn more about tryouts, medical requirements, and schedules...
Learn more and see if you are interested in one of the many activities and clubs available.
With school starting soon, please visit the health services website for immunization requirements.
View your bus information or student drop off instructions before school starts...
Clever Login
Clever is a portal that gives students' access to some sites and databases including Discovery Education which has a lot of media for research and projects.
Citation creation tool.
Hamilton East Public Library's Online Databases
Inspire is a collection of databases valuable to research that is free to anyone in the state of Indiana.
Purdue OWL's MLA Formatting Style Guide
World Book Online
When using this online encyclopedia on school wi-fi, students do not have to login. If using it outside of school, there is a login students can get from a teacher or the library.


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Histone modifications are a group of post-translational modifications on histones which can alter chromatin structure and affect gene expression. Histone ubiquitination is a histone modification found in particular on histone H2A and H2B. Histone ubiquitination can be reversed by ubiquitin-specific proteases (UBP). Here, we describe an in vivo assay for histone deubiquitination activity. After infiltrating UBP12 into Nicotiana benthamiana leaves, H2Aub was visualized by immunocytochemistry. Nicotiana benthamiana leaves, which show high agro infiltration efficiency, were used for transient UBP12 expression for a labor- and time-saving protocol. Reduced H2Aub levels indicated histone deubiquitination activity of UBP12. The clear visualization of nuclei of N. benthamiana leaves makes this method able to easily measure the level of histone modification in vivo by using specific antibodies, providing robust clues of protein function. Thus, this protocol is a powerful complementation to in vitro assays of histone deubiquitination activity.
Keywords: Histone deubiquitination, Immunocytochemistry, H2Aub, in vivo
Histone modifications play important roles in regulating chromatin structure and gene expression. Best studied histone modifications include methylation, acetylation, phosphorylation, ubiquitination and sumoylation. However, enzymes introducing or removing specific histone modifications are not always known. Powerful in vitro assays can establish the catalytic potential of histone modifying enzymes but in vivo methods are desirable to confirm that in vitro specificity reflects in vivo activity. Here, we describe a flexible protocol to test activity of histone modifying enzymes in the plant N. benthamiana. Although we used the protocol to test activity of ubiquitin specific protease (UBP) on ubiquitylated H2A, it can also easily be adopted to other histone modifications for which specific antibodies are available.
Materials and Reagents
Data analysis
Check several fields containing together at least 50 nuclei for statistical analysis. Choose fields with similar background signal because some regions on the slide may have high background from inadequate rinsing. Figure 2 shows a strategy for quantitative analysis of assay results.
Look for nuclei by DAPI signal (Figures 1A and 1B, left panel) and use the CFP signal to select nuclei that were successfully transfected with pUBC-H3.3-CFP (control construct) or pUBC-UBP12-CFP (test construct) (Figures 1A and 1B, center panel). Compare transfection rates (Figure 2A); ideally, transfection rates should be similar for control and test constructs. Count the number of nuclei with fluorescent signals for H2Aub or H4 (Figures 1A and 1B, right panel). Detection efficiency of immunocytochemistry can be expressed as number of H4-positive nuclei as percentage of all transfected nuclei (Figure 2B). Similar values indicate that the detection process (fixing, staining, washing etc.) was comparable for control and test constructs. The effects of control and test constructs on target PTM can be seen in a comparison of the percentages of PTM-positive nuclei after transfection (Figure 2C). Smaller values for the test than for the control construct indicate that the test construct specifically reduced the target PTM. An alternative display is to compare the relative frequencies of PTM-positive nuclei (Figure 2D). For this, the frequency of PTM-positive nuclei after transfection with the control construct is set as 1 and the frequency of PTM-positive nuclei after transfection with the test construct is expressed relative to it. A well-working test construct should affect the target PTM and thus show a relative frequency of PTM-positive nuclei < 1. This display directly shows the effect size of the transfected test construct and is recommended when transfection or detection rates differ; results should be compared across several experiments or when multiple test constructs are included.
Figure 2. Strategy for quantitative analysis of assay results for a histone posttranslational modification (PTM; here: H2Aub). Control and test constructs (here: pUBC-H3.3-CFP and pUBC-UBP12-CFP) are represented by light grey and dark grey bars, respectively. A. Transfection rate, i.e., number of CFP-positive nuclei as percentage of all detected nuclei. Similar bar heights indicate similar transfection rates for control and test constructs. B. Detection efficiency of immunocytochemistry, i.e., number of H4-positive nuclei as percentage of all transfected nuclei. Similar bar heights indicate that the detection process (fixing, staining, washing etc.) was similar for control and test constructs. C. Effect of control and test construct on target PTM, i.e., number of PTM-positive nuclei as percentage of all transfected nuclei. Smaller values for the test than for the control construct indicate that the test construct specifically reduced the target PTM. D. Alternative display of the effect of control and test constructs on target PTM using relative frequencies. The frequency of PTM-positive nuclei transfected with the pUBC-H3.3-CFP control construct was set to 1 and the frequency of PTM-positive nuclei transfected with the pUBC-UBP12-CFP test construct expressed relative to this. A well-working test construct (here: pUBC-UBP12-CFP) should affect the target PTM and thus result in a relative intensity < 1.
Seeds of N. benthamiana and the construct harboring viral RNA silencing suppressor p19 were kindly provided by E. Savenkov (SLU, Uppsala). This protocol was developed from the following published paper: Derkacheva et al. (2016). This work was supported by a grant from the Knut-and-Alice-Wallenberg Foundation. The authors declare no conflicts of interest or competing interests.
Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
You are highly recommended to post your data including images for the troubleshooting.
You are highly recommended to post your data including images for the troubleshooting.


text: You now have more time to participate in a free, secure assessment that can help your organization gain lasting safety benefits! The deadline for data submission to ISMP's national Gap Analysis of Safe IV Push Medication Practices has been extended to April 30, 2019. The assessment is short and easy to complete, and participants will immediately obtain a score. Don't miss out—access the assessment tool at: www.ismp.org/resources/gap-analysis-tool-safe-iv-push-medication-practices.


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These necklaces are perfect for a hunting family with a son! The buck, doe and spike interlock perfectly with each other. Each pendant will come on a very high quality stainless steel chain.
Would you like your design cut on a special year OR state quarter? (State Quarters only come in one year.) Just put your request in your order comments. Make sure and tell us if you want it cut on the heads side or tails(state side).
If you like the design but want it cut in silver, check out the silver quarter upgrade under the coin type option bar.
Want to see a baby girl deer instead?
Also, see all of our Buck and Doe design variations here.
If you like our work, please check out our Facebook page. We regularly post news and new designs there. We also answer questions about our work and all sorts of design possibilities.
Learn more about our shop and process.


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Green Arrow: Cross Roads is a ten-part story published from 1993-94. It was written by Kevin Dooley, Alan Grant and Doug Moench, illustrated by Jim Aparo, Michael Netzer and Eduardo Barreto. Mike Grell had just left the comic a
Green Arrow: Cross Roads is a ten-part story published from 1993-94. It was written by Kevin Dooley, Alan Grant and Doug Moench, illustrated by Jim Aparo, Michael Netzer and Eduardo Barreto. Mike Grell had just left the comic after eighty issues. During that long run the character's connections to the fantastic nature of the DC Universe had been removed, and he had been taken down to a completely grounded level. The plot is a series of loosely connected team-up stories dealing with Oliver Queen's return to the superhero community and lifestyle.
Dinah Lance has broken up with him after she caught Oliver kissing another woman.[1] For the first time since he's been there, there's a superhuman conflict in Seattle... a fight between Nuklon and Shrapnel. Green Arrow intervenes and controls the situation, but in the mess afterward, he decides there's nothing more for him here and he's going to leave Seattle for good.[2]
|This article is incomplete.
The story ends on a stirring metaphor during Zero Hour as reality itself begins to tear apart and existence itself is threatened. Time splits around him into two alternate paths as he's chasing a criminal down the street. In one version he successfully takes the thug down, but in the other he hesitates for a second and is shot repeatedly with the man's gun. Oliver is left standing over his own dead body, seeing that he can't hesitate in what he does... even for a second. Batman arrives to tells him they need him and he immediately answers the call.[3]
- Green Arrow #81
- Green Arrow #82
- Green Arrow #83
- Green Arrow #84
- Green Arrow #85
- Green Arrow #86
- Green Arrow #87
- Green Arrow #88
- Green Arrow #89
- Green Arrow #90
Items: None known.
Vehicles: None known.
Weapons: None known.
- No special notes.
- No trivia.
Recommended Reading
- More Fun Comics #73-107
- Green Arrow (Volume 1)
- Green Arrow (Volume 2)
- Green Arrow (Volume 3)
- Green Arrow (Volume 4)
- Green Arrow and Black Canary
- Green Arrow: The Wonder Year
- Green Arrow: The Longbow Hunters
- Green Arrow: Year One
- Connor Hawke: Dragon's Blood
- Black Canary (Volume 1)
- Black Canary (Volume 2)
- Black Canary (Volume 3)
Links and References
|Green Arrow Storyline
This event or storyline specifically revolves around Green Arrow, or Team Arrow as a whole. This template will automatically categorize articles that include it into the Green Arrow Storylines category.


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BHC RHODES announced Mr. Randy Gorton, P.E. was selected as a Vice President of the company. Gorton leads the Public Works group which provides civil engineering services to customers in and around the Kansas City metro area. Gorton is charged with enhancing project processes and efficiencies for customers, as well as business development. He is a graduate of the ACEC-KS Emerging Leader program and has received the BHC RHODES' Customer Care award multiple times for his service to customers.
BHC RHODES Promotes Randy Gorton to Vice President
Tags: BHC RHODES, Civil Engineering, Public Works
Leave a Reply


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This tutorial was written by me on August 26th, 2012. Any resemblance to any other tutorial or tag is unintentional and is pure coincidence. Please do not claim my work as your own and do not post to any other website, blog or forum without permission from me. It is written with the assumption that you have a working knowledge of PSP and its components. It was written for PSP 9.
I recommend that you read through this or any tutorial you are attempting before starting it so you will know what to expect and how to get organized.
As with any of my tutorials when a plugin is required, change only the settings I recommend and leave the others on the default settings.
What you will need to accomplish this tutorial is this sexy tube by Barbara Jensen which you can purchase on her site HERE. This stunningly romantic type kit called Portrait of a Rose by Tootypup Scrapz which you can purchase HERE at Designer Scraps. Please support the arts and DO NOT use Barbara's tubes without a license and don't share tubes or this scrap kit. Two brushes I have supplied HERE. Plugins are Alien Skin Eye Candy 5/Impact, Eye Candy 4000, Toadies and Penta. Font is A&S Edoras Chisel.
Let's get started....
First let's get your brushes into PSP. Open both I supplied into PSP then go up to File/Export/Custom Brush. Name and save them.
Now start a 700 x 700 new white image. Copy and paste Vintage Frame Plain 2 into your new image and then resize it 40%. Copy and paste paper of choice beneath this frame layer...I used Paper 1. Resize the paper 50% and then use your Raster Deform tool and drag the edges of the paper to fit into your frame. For coloring my paper I went to Adjust/Hue and Saturation/Colorize and used these settings:
Add a light drop shadow to your frame.
Next use your Preset tool and draw two rectangles beneath the paper layer...one black and one pink. Paste the close up tube and fit her face into the black rectangle. Then in your layer palette select the black rectangle layer and then grab your Magic Wand with a feather of 50%. Go back to the close up tube layer and then up to Selections/Invert and hit delete several times. Your work should appear like this so far....
I used my Raster Deform tool and resized my frame by stretching it up a bit.
At this point copy and paste your tube into your working image on the top of your canvas. Resize it 70% and place it to the left of your frame. For the bottom of your tube on her legs, use your Selection tool with a feather of about 50%, select the very tip of her legs and hit delete.
Now go to Adjust/Automatic Saturation Enhancement and use these settings:
Then go to Effects/Plugins/Alien Skin Eye Candy 5/Impact/Perspective Shadow and use these settings:
(You can tweak your shadows by moving the nodes around in the preview window. To move one node at a time hold down your CTRL key. You can move the whole shadow by holding down your right mouse button and clicking in the middle of the shadow. It's very important to think about how a shadow would be cast realistically and how dark or how light it should be. This plugin is one of my favorites and one I use with nearly every single one of my tags.)
Using my image as a reference begin to copy and paste the rest of the elements into your canvas. For precise sizing I use my Raster Deform tool. It also near eliminates the distortion or pixelation that happens when resizing in percentages at times, depending how large or how small you go. Don't forget to add slight drop shadows to each and for the elements you want to appear more dimensional use the Perspective Shadow plugin.
For what appears to be a mask is indeed the brushes. Use brush 1 and paint a few times down one side, then duplicate and mirror to make your mask...merge the two layers together. Add another layer and use brush 2 and repeat what you just did with brush 1.
At this point hide the background layer and the brushes layers in your layer palette and then Merge/Visible the remaining layers. Unhide the hidden layers.
Then go back up to Effects/Plugins/Toadies/Blur 'em and use these settings:
Next, in your layer palette select the duplicated merged layers and then go to Effects/Plugins/Eye Candy 4000/Corona and use these settings:
Now for you name once you've type it (for crisp edges on your fonts, set your Antialias to Sharp) go to Effects/Plugins/Alien Skin Eye Candy 5/Impact/Chrome and use these settings:
Duplicate your name and on the duplicated layer go up to Adjust/Hue and Saturation/Colorize and use the same settings as you did for your paper. Merge the two name layers together, rotate 90 degrees to the left and then give them a drop shadow by going to Effects/3D Effects/Drop Shadow and use these settings:
Now rotate back to the right 90 degrees and then go to Effects/Plugins/Eye Candy 4000/Corona and use these settings:
Add your copyright. Take care that your copyright is legal and legible. For a neat and professional-looking copyright I use pixel or copy fonts...some of these fonts are also called Ceriph fonts. You can google and find hundreds of these fonts. Most of them work best with your Antialias set to off and at size 6...although, I have found that if I am putting these fonts on a curve setting my antialias to sharp or smooth will make it look better. Resize and save as a .png or .jpeg and you're done.
I'm glad you tried my tutorial and I hope you had fun with it and maybe learned something to hone your PSP skills. Please feel free to contact me with any questions you have about any of my tutorials and please let me know about issues with broken links, etc.


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Do you extremely interested in an anime but your friend around you seldom know?
Are there any famous anime you know but you didn't see them for some reason?
Here is a chance to share!
You can recommend your favorite anime to others, so some people will go and see them, or you will find someone also interested in that.
You can share your interesting reasons fir why you don't see that famous anime.
Whatever you share, your word will be post on this website for everyone to see!
Join our campaign and share your experience.
To know how to join the campaign, read this post for details.


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The Specialist Buyers Guide for the Renewable Energy Industry
Found Under:Air Conditioning
Marstair is a forward-thinking manufacturer, it's product development is focused on energy efficient and environmentally sustainable designs. We offer cutting edge refrigeration and air conditioning products and our onsite engineering team allow us to offer bespoke systems to suit the most challenging of requirements.
Marstair offer a wide range of refrigeration and specialist air conditioning products for your Offshore, Onshore and localised Vessel applications including extreme high and low ambient temperatures. Marstair's ATEX split systems are specially designed for installation in explosive atmospheres (Zone 1 & Zone 2) in marine and harsh environments. These high specification robust systems are based on proven Marstair's equipment designs over the last 45 years.
Typical applications include.
Quartz the sister division has a history of excellence and quality in the HVAC industry, at the cutting edge of chilled water and fan coil cooling. Our expertise ensure Quartz remain one of the main suppliers of chilled water air conditioning in the UK market.
Both Marstair & Quartz are accredited to: BSI ISO 14001:2015 for Environmental Management and BSI ISO 9001:2015 for Quality Management.


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In today's fast paced, I want it now world, millions of people are searching for the proverbial "magic bullet". They want this "magic bullet" to immediately improve their health, business growth, intimacy and general happiness. Many of my clients initially seem quite receptive to any "shortcut" that would enable them to avoid putting in the hard work and discipline needed to achieve the success they seek. I routinely advise my clients that success comes to those who are willing to roll up their sleeves and engage in the SUCCESS PROCESS. Ironically, five of the key elements inherent to the SUCCESS PROCESS just so happen to spell out the word MAGIC. Let's take a closer look…
MOTIVATION. Motivation is an inside job, either you're motivated or your not. There is no "magic bullet" that can motivate someone to do something they're not dedicated to accomplishing. Find your "WHY", design a plan, secure an accountability partner and get started!
ACTION. We must be willing to take massive action. Action doesn't come in a "pill" or secret potion, it is born from BIG DREAMS. The bigger our dreams the more action we will be willing to take. Action builds muscles of courage and boosts confidence.
GOALS. Our attainment of a significant goal is a profound experience. Who we become as a person from the time we set our goal to the moment our goal is realized (the process) is the true "magic" of the goal. "Magic bullets" are not an option, hard work and sacrifice is the determining factor.
INTUITION. Tapping into and trusting our inner voice is THE grounding principle of the success process. All of us know how we truly feel in every interaction or situation we encounter. Our ability to communicate this truth sets us apart from those who are in a continual search for the "magic bullet". When we are in touch with our authentic self we have little use for outside chatter. We take full responsibility for our RESULTS.
COMMITMENT. Without a high level of commitment we will never achieve anything of real significance. We must be committed to our plan of action. Our dedicated commitment to ourselves and our plan will get us through set backs, rejection, failure and our commitment will accelerate our resolve.
There is no such thing as a "magic bullet". The only "magic" lies within the process. The process goes along way in determining who we become. LEARN TO EMBRACE THE PROCESS.
I look forward to your thoughts and feedback.


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Laver Cup featuring Roger Federer draws criticism from St. Petersburg Open
by LUIGI GATTO | VIEW 7475
The St. Petersburg Open enjoyed a very successful 2018 edition last month as Dominic Thiem defeated Martin Klizan in a straight-set final. In an exclusive interview with Tennis World USA, the Tournament Executive Director Natalia Kamelzon spoke about the event overall, starting from the fact that Laver Cup is played during the same week as St.
Petersburg. Does it affect the Russian event field? Many top players compete in Chicago, including Novak Djokovic. As per reports, bringing the Serb at the St. Petersburg in 2018 was one of your goals. 'Of course, I do not like that Laver Cup date coincides with quarter-finals, semifinals and also finals of St.
Petersburg Open because it has an impact on our entry list and also the Metz tournament', Kamelzon admitted. 'This year's St. Petersburg Open was our fourth one in a row as Formula TX and we always manage to bring in good players.
So we are not worried by being "closer" to Laver Cup, that is a promotional event, while the event of our is part of the ATP Tour and so you can earn important points on the ATP Rankings. The St. Petersburg Open field speaks for itself - and we are happy with it.
Stan Wawrinka, Dominic Thiem, Roberto Bautista Agut and many others are part of it. Yeah, we negotiated with Novak Djokovic, but it's up to him to choose.' Kamelzon also did not deny that Davis Cup may move to September after the US Open.
If it happens, the Russian event would take place one week later, it means in late September, and the Asian swing would just be played in October. Nick Kyrgios never played the tournament. Could it be an option for the event in the future? 'In 2018 we had the Canadian Denis Shapovalov who has Russian grassroots, playing a unique backhand.
Even the Russian Daniil Medvedev, another Next Gen player, who reached the quarter-finals here and recently won the Tokyo title. Nick will play the Kremlin Cup in Moscow because he got a wildcard, so I imagine he would like to potentially play in St.
Petersburg as well. If he asks us, we will evaluate.' ALSO READ: Rafael Nadal: Worse players than David Ferrer won a Grand Slam title


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Folliculitis is a skin condition where hair follicles become inflamed and infected. It occurs most frequently in places that are shaved such as the beard area and legs. It can also appear on arms, back, and buttocks. Looking very similar to acne, it can sometimes turn into a worse and more serious skin condition such as a staph infection.
Possible long-term side effects of folliculitis are:
- Permanent hair loss from the destruction of hair follicles
- Large, itchy patches of infected skin
- Boils under the skin
The causes of folliculitis may vary, much like the causes of acne (besides hereditary factors, acne can be caused by certain foods, products, and exercising without wiping sweat off.) Some of the most common causes for folliculitis include:
- Virus or fungi
- Staph infection
- Injuries to the skin
- Tight clothing
- Damaged follicles
- Inflammatory skin conditions such as acne
- Friction from shaving
- Heat and sweat from wearing rubber gloves or waders
Symptoms of folliculitis can be similar to acne, which is why they are often treated in a similar manner. The symptoms include the following:
- Tenderness
- Pain
- Inflamed skin and scalp
- Bumps with pus
- Small boils
- Severe acne lesions
- Barber's itch
- Pain associated with blisters
- Crusting of pimple
- Chronic red face
- A large swollen bump or mass
- Itchy, burning skin
Prevention is your best weapon against folliculitis. With that being said, waiting too long to have this skin condition checked out can result in the more permanent side-effects like those mentioned above. Here is how to prevent folliculitis:
- Keep skin clean
- Apply mandelic acid such as this Exfoliating Serum Lite or this Exfoliating Serum 2, both of which kill the bacteria before it gets into the open follicles
- Use antibacterial soap for handwashing
- Go to a Skincare and Acne Clinic for a consultation
- Drink lots of water
- Avoid friction from tight clothing
- Use clean 1 bladed razors for shaving, throw out every other use and use new
Once you know the causes of folliculitis, it can be simple to look out for and get the right remedies. Folliculitis does not have to be a lifelong problem for you. Especially if it is caught early on, your skin can be saved from itchy red bumps and pus-filled blisters. Use the information above to help your skin condition or simply give us a call at 801-800-6602 today.


text: GELLERMAN: Well the World Bank used to finance many big dam projects, but citizen action and environmental concerns have changed that policy. Yet many developing nations still see hydropower as an untapped renewable source of clean energy. International Rivers monitors dam building around the world. Patrick McCully is executive director of the conservation group, and he says there's no shortage of cash to fund big dam projects. MCCULLY: What's happened is that countries like Brazil, India, China, now they have a lot more of their own resources so they don't need international money so much, and one other really major factor in dam building around the world at the moment is that China actually is going around the world and writing checks for big dams without any types of environmental or social policies, and in many ways replacing the role that the World Bank and other Western funders used to play. GELLERMAN: Why would a country like China be interested in financing these kind of projects? MCCULLY: Partly it's promoting their own industries. So when China is funding, for example, a big dam in Sudan that they've been building which is very controversial, and their Chinese construction companies get all the contracts, so that's an obvious reason for the Chinese to want to promote it. But there's also geopolitical reasons. So, again, use Sudan as the example, China is very keen to have good relations with Sudan because they want to get Sudanese oil, so building a dam is a good way of cementing relationships. GELLERMAN: It's ironic that worldwide there's this resurgence of dam building, and yet here in the United States, there are a lot of river restoration projects and the removal of dams, going back to, well, about 1999. MCCULLY: Yeah, for quite a few years now, we've had more dams being removed from the rivers of the U.S. than are being built. Something similar is happening in various parts of Europe. In other parts of the world there are a lot of dams being built; there is a very big global dam lobby. They have a lot of different reasons for promoting big dam projects. Obviously there is a massive demand for electricity around the world, and growing very fast. But we can see some of their projects being promoted at the moment are not very rational, let's say, and the great example of that is this Grand Inga project in the Congo in central Africa, where you have a dam with a price tag of about 80 billion dollars. GELLERMAN: Yeah, I understand that would be the largest dam in the world ? much bigger than the Three Gorges Dam in China. MCCULLY: Yea, by far, more than twice as big as Three Gorges Dam, and with a price tag several times as big, being built in one of the most unstable and conflict-ridden and corrupt countries in the world. The record of these big projects is that they spark off lots of corruption and can lead to conflict of various types. GELLERMAN: What role, if any, do carbon offsets play in the construction of dams ? that is, if they build a dam, they get credit towards polluting carbon dioxide in other places? MCCULLY: Yeah, that's a very good question. There's a massive scam going on at the moment whereby hundreds of dams around the world have received or are applying for carbon credits under the UN's Clean Development Mechanism, which is part of the Kyoto Protocol. The whole idea of these carbon credits is that they're supposed to go to projects which wouldn't happen otherwise, so they're supposed to represent genuine emission reductions, and then polluters in industrialized countries that have ratified the Kyoto Protocol ? they can use those offsets, so they don't have to reduce their own emissions. But these credits are all going to dams that are being built anyway ? most of them are completed by the time they actually get the credits, so there's no way that the credits are in any way making the dams happen. Basically the consumers and taxpayers of Europe and Japan and the global climate are suffering because of this system. GELLERMAN: But Mr. McCully, what's wrong with a dam? I mean, they don't emit greenhouse gases, they do produce electric power ? what's wrong with that? MCCULLY: Well, biggest problem usually is these big dam projects is that they displace huge numbers of people and cause great harm to their livelihoods. When you build these great mega-projects, their power lines go off to the cities and the mines and the industries, and they don't provide benefits to local communities, and they do great harm to riverine ecosystems. But beyond that, the argument that they are by their nature climate friendly is very simplistic. In fact the big reservoirs in the tropics can have very large greenhouse gas emissions, because basically flooded vegetation and soils, and that produces methane, and that's a very powerful greenhouse gas. So the big Amazonian reservoirs, for example, can have much higher global warming impact than fossil fuels, even than coal plants. GELLERMAN: I'm wondering, personally, you know, you're up against some of the biggest corporations and countries in the world. Do you ever feel like that little Dutch boy with the finger in the dike, you know, trying to hold this thing back? MCCULLY: (chuckles) Yeah, sometimes it's ? sometimes it's easy to feel a bit overwhelmed, but at the same time we have a great global network of organizations that we work with. There's also in the rise of renewable energy, falling price of solar, the increasing feasibility of wind power in many places. So the clean energy alternatives are much more viable that they used to be, which is a great thing for us. GELLERMAN: Well, Mr. McCully, thanks very much. I appreciate it. MCCULLY: It's a pleasure. GELLERMAN: Patrick McCully is executive director of the conservation group International Rivers.


text:
- +31 (0) 224-55 1686
- email@example.com
Closed because of internal relocation16-12-2015
This year, FB Hout will not be reachable by phone or e-mail from the 25th of December 2015 until the 4th of January 2016. Our distribution office will undergo an internal relocation.
We are proud to inform you that as of Monday the 4th of January, we will be assisting you from our brand-new office. This new office fully reflects FB Hout. Our products have been applied in all possible manners.
Follow us in LinkedIn, Facebook and Twitter to get more information about our renovation and other news that cannot be missed!
On behalf of FB Hout, we wish you a Merry Christmas and a Happy New Year and we hope to meet you at the beginning of 2016 at the Domotex fair in Hannover! For free tickets you can still reach us at firstname.lastname@example.com.


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GoWise USA 8-in-1 Electric Air Fryer 3.7 Qt Review
There's nothing like a big price savings to stop one in their tracks and that's the biggest reason I uncovered in my …
All about countertop GoWise USA hot air fryers.
Read GoWise air fryers reviews, pros and cons.
What is an air fryer?
The hot air fryer uses rapidly circulating hot air to cook food instead of hot oil.
GoWise air fryers make use of rapid air circulation technology to cook food without the need for additional oil.
Food is tender and juicy inside and crispy outside.


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Change Can Be Difficult, Part 2
(My sister is checking out at a popular retail store. The total comes out to $14.01. My sister hands the cashier $15.)
Sister: "Hang on, I have a penny."
(The cashier ignores her and starts counting out change while she pulls out a penny.)
Sister: "Here, just give me a dollar back."
Cashier: "What?"
Sister: "Take this penny, and then just give me a dollar back."
Cashier: "I already calculated the change in the machine. My register won't be balanced."
Sister: "Okay, but if I give you a penny, and you give me a dollar it will still be balanced."
Cashier: "But the register says 99 cents!"
Sister: "I know, so you give me a dollar, and I give you a penny, it evens out."
(The cashier looks visibly distraught and hands my sister a bunch of change.)
Cashier: "I'm really sorry. I don't know what you want me to do!"


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On Sept. 28 a battery and theft by taking complaint was filed at a Commerce Street residence. According to reports, a woman at Morgan Medical Center initially alleged that a man had sexually assaulted her. The woman then told the officer that the man had not sexually assaulted her but had struck her in the face and and stole her Klonopin and Norco prescriptions. The woman said she could not remember anything that had happened in the last 24 hours, however, she said a neighbor initially told her that she had struck the complainant in the face but then said she said that to cover for the man who allegedly struck the woman in the face.
On Sept. 28 Damonte D Powell, 20, Monroe was arrested and charged with open container. According to reports, an officer conducting patrol on Pearl Street allegedly observed at 10 p.m. several individuals "laying drag" and driving recklessly near Morgan Circle. The officer observed a Jeep accelerate recklessly toward Georgia Highway 83. The officer pursued and after the officer engaged his emergency lights, the Jeep tuned into the north entrance of Morgan Circle and stopped. The driver leapt from the vehicle and, reports state, a handgun fell from the male's waistband. The driver fled on foot. Powell, a passenger in the vehicle stepped out and was detained. Reports state that an open 750 ml bottle of cognac was located in the vehicle. Powell told officers he did not know the driver's name but had enlisted him to drive at the Mad Town event because, reports state, Powell felt he was too intoxicated to drive.
On Sept. 27 a theft by taking complaint was filed at Flash Foods, Eatonton Road. According to reports, an employee alleged that a man entered the store and purchased gas and a case of Natural Light beer. The employee alleged that the man left the store and took a bag of ice valued at $2.66 without paying for the ice. Reports state that the male looked directly at the employee as he took the ice. The man's license tag was captured. Officers produced an image of the suspect's driver's license and the employee positively identified the suspect.
On Sept. 26 an information report was filed at Morgan Medical Center, Lions Club Road. According to reports, an employee alleged that a man with a psychiatric hold had walked out of the facility, gotten into a white Chevrolet Tahoe and left. The officer obtained the man's address and county units were able to locate the man at his last known address and return him to the hospital.
On Sept. 24 an information report was filed at the Deerfield Inn, Eatonton Road. According to reports, an employee showed an officer a black Nissan Pathfinder that had been parked at the motel for several months. The officer examined the vehicle and learned that the vehicle had been reported stolen out of Bibb County on July 10.
On Sept. 18 a criminal trespass complaint was filed at a Penor Street residence. According to reports, a woman alleged that her sister's boyfriend had broken her son's IPhone 7. She also alleged that the boyfriend struck her son with a stick. The boyfriend alleged that he was walking by the complainant's house when the son came to the edge of the driveway with pepper spray and a knife and threatened the boyfriend and then pepper sprayed the boyfriend. The boyfriend said he then swiped the son's phone from the parked truck and left. He alleged that the son followed him and pepper sprayed him again and attempted to cut him with a knife. The woman valued the damaged phone at $250.42.


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Being a philanthropist is tough work
Pine and The Pineapple Fund have taken the cryptocurrency and charity world by storm. Pine, about whom we know nothing, has pledged to donate $86 million to charities and non-profit organisations. (see earlier story)
The only information we have on this anonymous philanthropist is that they got on the bitcoin train at the beginning and they now have more money than they need; hence their generosity.
CCN asked would they also donate to start up charities and non-profits as opposed to limiting their largesse to registered charities. However, they replied this would not be possible as it would require The Pineapple Fund to be run on a much larger scale and for them to have greater knowledge in these areas and to spend more time which they sadly don't have.
Pine added: "We originally had plans to create a 'charity accelerator' where we would offer grants and more importantly, mentors, support, and peers to passionate individuals who want to make a difference in this world. In order to do that right, we'd need significant time and more experience in the non-profit space, both of which we sadly don't have. We've been sticking to more established charities with a proven track record so far."
While they have three main criteria for choosing a worthy cause – how impactful their work is (especially on an international scale), what new innovative skills they are bringing to the table, and how efficient and sustainable they are – another important factor is their gut feeling.
When CCN asked what gives them a positive gut feeling Pine replied: "I personally look things like how articulate the charity presents itself, and look at their actual programs to gauge if I feel that is an effective way to achieve what they set out to achieve. I particularly look for more innovative approaches."
The Pineapple Fund has closed its application window as they have received over 10,000 applications and thousands more emails. They are taking their time to process through the emails to pick out the charities that tick the boxes for donations.
Pine said: "That is not a success metric in itself, but we're happy about how we have an incredibly diverse number of causes to evaluate and fund."
Due to the large volume of applications and emails they have received, they have closed the application process and have no plans to reopen it as they feel they will have plenty of charities and non-profits to divide their remaining bitcoin between.
We shall have to wait for the next bitcoin millionaire to open a new charitable fund for those who did not get their entries in on time.


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Skip to 0 minutes and 10 seconds GFR estimation. Direct measurement of GFR by endogenous creatinine is the most accurate but inconvenient to do. On the other hand, use of radio-labeled agent is undesirable for environmental reason and for safety reason. And therefore we would like to use a serum marker if possible to estimate GFR. And there are several formulas available for this purpose. Including Cockroft-Gault method, MDRD, Jelliffe Method and the Schwartz formula. And we will talk about each and every one of them. First the Cockroft and Gault equation. Now this equation says, creatinine clearance is equal to 140 minus age times body weight divided by 72 times serum creatinine.
Skip to 1 minute and 19 seconds So here again Clcr is the creatinine clearance, Ccr is the serum creatinine, age in year and body weight in kilogram. And the body weight is gross weight in the original formula. And very interestingly, based on this formula. The maximum life expectancy is about 140 years for men or women. Now the Cockroft-Gault Estimate. The formula was published in 1976, and it was derived from 249 normal white males age 18-92. The original formula was based on gross weight. And the correction factor is 0.85 for female subject, assuming that there's a 15% difference in muscle mass between male and the female subject. And some physicians actually believe that this formula provides a more accurate result than the 24-hour creatinine clearance.
Skip to 2 minutes and 31 seconds Because of the potential under collection of urine particularly in an outpatient situation. There are some ground rules for using the Cockroft-Gault equation. First, use gross weight if height is not available. Use ideal body weight if both the weight and height are available. This is to avoid overestimated particularly in obese patient. If the gross weight is less than ideal body weight, then use the gross weight. This could be the situation in malnutrition or muscle wasting in AIDS patient. This provide reasonably accurate estimate for mild to normal kidney function. Ideal body weight calculation. Ideal body weight can be calculated by these formulas for male. And for female. And you can find this equations of formula from the literature source.
Skip to 3 minutes and 42 seconds Here I like to introduce another body weight term. It's called adjusted body weight. Adjusted body weight is for obese patient whose actual weight is greater than 30% of idea body weight. And here is the formula. Adjusted body weight is equal to ideal body weight plus 0.4 times total body weight minus ideal body weight. The difference between total body weight and the ideal body weight essentially is the fat content. Therefore adjusted body weight takes into consideration ideal body weight and the 40% of the fat content. Adjusted body weight is used for dosing adjustment. Ideal body weight is used for both creatinine clearance estimation and the dosing adjustment. Another body weight term.
Skip to 4 minutes and 43 seconds Lean body weight is rarely used for clinical application except as a weight loss measure in the gym or gymnastics
GFR Estimation : Cockroft-Gault Equation
Continuing from the previous step, Prof. Lee illustrates Cockroft-Gault Equation this time.
To begin with, we have to know that direct measurement of GFR by endogenous creatinine is most accurate but inconvenient, and the use of radio-labeled agent is undesirable. Consequently, we would use Ccr to estimate GFR.
There are four formulas available, including Cockroft-Gault, MDRD, Jelliffe Method, and Schwart. Prof. Lee clarifies Cockroft-Gault first since it is the most common one.
While using this formula, it is necessary for us to know that correction factor is 0.85 for female, assumed 15% lower in muscle mass.
Ultimately, we need to know when to use ideal or adjusted body weight, and we must understand how to calculate ideal body weight and adjusted body weight.


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|Short-term effects of physical activity, air pollution and their interaction on the cardiovascular and respiratory system
|Publication Type
|Journal Article
|Year of Publication
|Laeremans M., Dons E., Avila-Palencia I., Carrasco-Turigas G., Orjuela J.P, Anaya E., Cole-Hunter T., de Nazelle A., Nieuwenhuijsen M., Standaert A., Van Poppel M., De Boever P., L. Panis I
|Environ IntEnvironment InternationalEnvironment International
|Date Published
|May 2
|ISBN Number
|Accession Number
|black carbon, Crae, FeNO, Heart rate variability, Lung function, Retina
Physical activity (PA) in urban environments may lead to increased inhalation of air pollutants. As PA and air pollution (AP) have respectively beneficial and detrimental effects on the cardiorespiratory system, the responses to these exposures can interact. Therefore, we assessed the short-term effects of PA, AP and their interaction on a set of subclinical cardiovascular and respiratory outcomes in a panel of healthy adults: heart rate variability (HRV), retinal vessel diameters, lung function and fractional exhaled nitric oxide (FeNO). One hundred twenty two participants measured their PA level and exposure to black carbon (BC), a marker of AP exposure, with wearable sensors during an unscripted week in three different seasons. The study was part of the PASTA project in three European cities (Antwerp: 41 participants, Barcelona: 41 participants, London: 40 participants). At the end of each measurement week, the health outcomes were evaluated. Responses to PA, BC and their interaction were assessed with mixed effect regression models. Separate models were used to account for a 2-h and 24-h time window. During the 2-h time window, HRV and lung function changed statistically significantly in response to PA (METhours) and logarithmic BC (%change). Changes in HRV marked an increased sympathetic tone with both PA (logarithmic LF/HF: +7%; p<0.01) and BC (logarithmic HF: -19%; p<0.05). In addition, PA provoked bronchodilation which was illustrated by a significant increase in lung function (FEV1: +15.63mL; p<0.05). While a BC %increase was associated with a significant lung function decrease (PEF: -0.10mL; p<0.05), the interaction indicated a potential protective effect of PA (p<0.05). We did not observe a response of the retinal vessel diameters. Most subclinical outcomes did not change in the 24-h time window (except for a few minor changes in LF/HF, FeNO and PEF). Our results on the separate and combined effects of short-term PA and AP exposure on subclinical markers of the cardiorespiratory system are relevant for public health. We provide insights on the physiological responses of multiple, complementary markers. This may move further research towards elucidating potential pathways to disease and the long-term clinical impact of the observed physiological changes.
|Short Title
|Environ IntEnviron. Int.
|Alternate Journal
|Environment international
Short-term effects of physical activity, air pollution and their interaction on the cardiovascular and respiratory system


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AT&T and Verizon Pre Plus and Pixi Plus available for free through HP
If you're the contract signing type, HP has the deal for you. Through their newly-launched HP Wireless Central store you can snag a Palm Pixi Plus or Pre Plus on AT&T or Verizon for the upfront sum of free, with free shipping via FedEx. Of course, you are still locking yourself into a two-year contract to save those bucks, but saved bucks are saved bucks, right? And if you spring for the Pre Plus (it's just as free as the Pixi Plus, so why wouldn't you?), HP will also throw in a free Renegade Bluetooth headset (valued at $49.99), an Axiom leather case ($29.99), and a car charger ($19.99).
Strangely, Sprint is still stubborn as usual on their pricing, with the more-than-a-year-old Pre still retailing for $99.99 and the Wi-Fi-less Pixi at $19.99. With all this cash you're saving, we're going to point you towards the PreCentral accessory store where you can pick up some more sweet gear, like a Touchstone charger or a better Bluetooth headset.
Source: HP Wireless Central, thanks to sepherous for the tip!


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Is Tasty plc a falling knife to catch after dropping 30% today?
Shares in casual-dining company Tasty (LSE: TAST) have plunged, after the company issued a relatively downbeat set of results for the 53 week period ended 1 January 2017.
During the period, the group continued to expand, increasing revenue by 28% year-on-year to just under £46m. This growth came off the back of the opening of 13 Wildwood restaurants during the year. Headline operating profit increased to £4.8m. However, the group reported a pre-tax loss for the period of £88,000 after taking exceptional costs of £3.6m. For the last comparable period, the company reported a pre-tax profit of £3.1m.
The £3.6m impairment charge is a result of management's decision to reconfigure some of its restaurants in order to bring performance in line with the rest of the estate. Management is also trying to improve staff productivity and reduce staff turnover by conducting training programs, as well as rebranding in certain areas. All of these changes, which should have a positive long term impact on group performance, are holding back short term trading.
Unexpected costs
As Tasty continues its expansion plan, it goes without saying that the company is going to have to book some exceptional growth charges. However, City analysts hadn't expected these charges to be so significant and were instead expecting charges to be more spread out.
Indeed, analysts had pencilled in earnings per share growth of 34% for the year ending 31 December 2016, on a pre-tax profit of £4.7m. Further growth was expected for 2017 and 2018, with pre-tax profit estimates of £6m and £7.6m pencilled in. From 4.6p for 2015, earnings per share had been expected to hit 10.1p by 2018. Now that Tasty has stumbled, it looks as if these profit forecasts may be revised significantly lower.
Unfortunately, the market had awarded Tasty a high growth multiple of 18.5 times forward earnings based on lofty city estimates for growth. Now that it appears that the company will struggle to meet these targets, investors have reacted by dumping shares in Tasty.
Multiple headwinds
Tasty's outlook may not improve any time soon. Headwinds for UK retail companies are building, with higher minimum wages, inflation and other rising employment costs all increasing the burden on employers. Meanwhile, higher prices from inflation and a more price conscious consumer are two factors hitting the supply-side. Put simply, doesn't look as if Tasty's outlook is going to improve significantly any time soon and this is bad news for the share price.
With headwinds against the business building and Tasty struggling to hit City forecasts for growth, it looks as if this is one falling knife investors should avoid. The shares may have further to fall before they fully reflect the company's new depressed outlook and it will take some time for the company to prove to the rest of the market that can return to its previous growth trajectory.
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Rupert Hargreaves has no position in any shares mentioned. The Motley Fool UK has recommended Tasty. We Fools don't all hold the same opinions, but we all believe that considering a diverse range of insights makes us better investors.