text:
Wikia Basics - Create an account
Sign up for an account • Edit your user profile • Preferences • Facebook Connect
Logged-in Wikia users are able to set some personal preferences which tailor the way they read, write and edit on Wikia to their particular style. These preferences are applicable across all Wikia communities, so the same preferences are applied on every community you visit. To access your preferences, move your mouse over your avatar at the top right of a page. A drop down menu will appear, and from here select "My preferences"
What are the different tabs for?Edit
This help page will walk you through the current preference options Wikia users have and explaining the effects of changing these options. We will address these in order from tab-to-tab.
Tabs are organized to clump together related preferences so that it is easier to find the relevant field to change. By default, there are five tabs on the Special:Preferences page.
Please realize that none of the changes you make to your preferences will be stored unless you press the "Save" button. You can also reset your preferences to the default settings you had when you registered for Wikia. This can not be undone, so choose wisely if you wish to reset.
My infoEdit
Basic informationEdit
- My username: Simply prints your username. You can not change your username through the preferences screen. If you wish to change your username, please see this page on username changes.
- My user groups: Lists the user groups you belong to.
- My gender: Not a required preference. By default, users are unspecified, though of course one can choose male or female.
- The purpose of this preference is for international languages in which proper nouns need a gender form.
- To learn how to set your gender for your User Profile, please visit Help:Profiles. Your profile gender is not set by this preference.
- My password: If you would like to change your password, please click the link in this field, which will lead you to Special:ChangePassword
- Existing Signature: Renders your signature, including all wikitext.
- Custom Signature: Where your signature can be changed.
- Wikitext you use will be converted when the signature is used unless you uncheck the "I want to use wikitext in my signature" box.
- Language: A dropdown menu where you can choose the language you wish to view the community in.
- Note: This applies to the interface on all communities, so while the content of a DE community still will be in German, if you have "EN - english" as your user language, you will see most of the messages and tools on the community in the English language.
- Date: Allows for users to change how they wish to see timestamps.
- Time zone: Allows for users to override the server time (which is in UTC) with the time zone of their own choice. Users can either select their geographical region to determine time zone from a dropdown list or can enter the offset manually using the next input box.
- This will not affect the timestamp in user signatures.
- Layout: Allows for users to see Wikia with the default Wikia skin or the Monobook skin.
- Landing Page: Users can either choose between going to the Main Page of a community or to WikiActivity when going directly to the community's URL from the address bar.
- Ads: Registered users on Wikia can hide most of the ads that our anonymous users see. Consider it a perk of membership. Some admins, though, like seeing all the ads on a community so they can understand the user experience for their anonymous readers.
The email tab allows users to change their email address on record with Wikia and then control the type and volume of emails they receive from Wikia.
- My email address: The field in which you see what current address you have on file. If you type in a new email address and save the page, you will be prompted to confirm your new email address.
- Email Confirmation: If you need to confirm your email address, you will see text informing you that your email is not confirmed and then offer a link to resend your confirmation email if it has been misplaced. If you are already email confirmed, you will be told you are confirmed and given a timestamp of when it was done.
Followed pagesEdit
To learn what followed pages are, please consult this help page on followed pages.
These preferences will determine if your account will automatically watch the page when you make one of four major changes to a page:
- When you edit a page
- When you create a page
- When you move a page
- When you delete a page
Email me when...Edit
Now that you have managed your subscriptions to a page through the followed page feature, you can also choose how much communication you receive about those pages. These are fairly self explanatory. Some quick notes:
- "Minorly changed" - means a user making a change to the page has checked the "minor edit" box.
- For communities with Message Walls or Forums enabled, you will see another section entitled "Message Wall and Forum" and a dropdown field to choose how you should be mailed.
- Wikias with the old user talk page system rely upon the previous box of "my user talk page is changed" for those followed pages emails.
Advanced optionsEdit
- Mark all pages from my Weekly Digest as visited - This option is only available if you're an admin of the community you're currently on. If you do not visit pages outlined in a Weekly Digest, you may be presented with the same list on your next Weekly Digest.
Disable all emailsEdit
Email communication is a critical tool that allows you to stay attuned on what is going on your favorite communities as well as on Wikia as a whole.
We presented you on this Email tab with plenty of options to suit the types and volume of emails you receive from Wikia. If you have concerns about email, we would suggest you review your general email preferences first.
That said, Wikia does respect your right to not be emailed from our network if you do not wish to be contacted by us. As such, if you check this box, you will not receive any emails from Wikia until the checkbox is unchecked.
Editing experienceEdit
- Preferred editor - see Help:Editing
- Widen the Source mode edit box to fill the entire screen - collapses the modules normally displayed to the right of the editor such as the button to add a photo or gallery and also moves the edit summary to the top of the editing bar.
- Mark all edits as "minor" by default
- Prompt me if I leave a blank edit summary
- Do not show syntax highlighting in Source mode
- Do not show link suggest in Source Mode - If you start typing an internal link ([[) followed by a few characters, a pop-up will appear that will let you autocomplete the page name you are trying to link to. Checking this box will disable that feature.
Advanced editing optionsEdit
This option allows you to choose which font style you'll see when you edit.
Starting an editEdit
- Show section edit links - Enables/disabled a clickable "edit" button for appearing next to any H2 or H3 headline.
- Edit sections by right clicking on section titles - Again applies to H2 or H3 headlines.
- Edit pages on double click - A JavaScript shortcut that allows you to simply double click on the body of a page to open the editing window.
- Disable Category module - Only applies if visual mode editing is disabled.
- Use a blank page as default for creating a new page
- Disable "Create a new article" flow - Not recommended.
Under the hoodEdit
This tab combines a number of highly technical, specialized preferences. If you are new to wikis and editing, you may want to leave this tab alone until you understand how most of these tools perform normally.
Recent Changes, Histories, and LogsEdit
- Days to show in recent changes: - Default: 7 days
- Number of Edits to Show by Default: - Default: 50 edits
- Hide minor edits in recent changes - suppresses all edits on the feed marked with "m".
Followed pages feedEdit
This will control followed pages behavior.
Advanced display optionsEdit
- Hide page content below diffs - By default, when viewing a history diff, the newer of the two versions will have its full page text and rendering displayed as well.
- Hide diff after performing a rollback - Removes an auto-redirect for users with the rollback right.
- Enable Go-Search - If your search term matches an existing page, it'll take you straight there.
- Search all namespaces by default
- Show hidden categories
- Hide my followed pages list from everybody - Makes your Special:Following list private.
- Enable View source on threads
Facebook ConnectEdit
The Facebook Connect extension has its own tab on your preferences. It is a tool that can help log in you to Wikia with the click of a button as well as more easily share information about your wikia to your Facebook friends and feed. Please click here to learn more about this tool.
If you have not yet connected your Wikia account to Facebook, one of the ways you can do so is going to this tab in Preferences and follow the link to start the connection process.
This is also the tab where you can choose to disconnect your account from Facebook Connect.
Non-default tabs & sectionsEdit
In addition to the default tabs & sections listed above, some extensions add extra preferences to your Preferences page. Please view the help pages linked to learn about these special preferences.
- Gadgets - The Gadgets extension will add a completely new tab to your Special:Preferences page.
- Patrolled editing - Adds a few options to your "Under the Hood" tab.
See alsoEdit
- Help:Managing Your Account - What else you can do with your account
- Help:Create an account - Further reasons to create accounts on Wikia.
Further help and feedbackEdit
- Browse and search other help pages at Help:Contents
- Check Help:Wikia Community Central for sources of further help and support
- Check Help:Contacting Wikia for how to report any errors or unclear steps in this article
- Learn how to use Wikia in {{#NewWindowLink:http://community.wikia.com/index.php?title=Wikia_University%7CWikia University}}: short how-to videos for all levels of experience
More help pages
Help Contents • Wikia Basics • Contributing • Designing Your Wikia
Managing Your Community • Managing Your Account • Getting Technical
es:Ayuda:Preferencias fr:Aide:Préférences ja:ヘルプ:個人設定 ko:도움말:사용자 환경 설정 nl:Help:Voorkeuren pl:Pomoc:Preferencje pt:Ajuda:Preferências ru:Справка:Настройки uk:Довідка:Налаштування zh:Help:參數設置


text:
Marlins striving to establish both sides of plate
MIAMI -- Sometimes when you are trying to establish the inside part of the plate, pitches get away. On those occasions, hitters tend get upset, like what transpired in the fourth inning of Miami's 11-2 win over the Nationals on Tuesday night.
The umpires issued warnings after Tom Koehler's fastball sailed up and in on Ian Desmond. It wasn't the first pitch to get away from Koehler, who buzzed Jayson Werth.
Koehler certainly wasn't trying to hit anyone, but he definitely was looking to establish pitching inside. For good reason, the Marlins had lost eight straight games, and over that stretch the pitching struggled.
Opponents were getting way too comfortable, and Koehler sought to establish his game plan.
"We've been beaten too many times late in the game over these last couple series, and that's because we haven't established in," Koehler said.
Koehler sent a message, and when Desmond took exception, catcher Jarrod Saltalamacchia came to his pitcher's defense.
"We were trying to pitch our game," Saltalamacchia said. "We've got to mix our pitches. Last few nights we were missing over the middle of the plate. [Tuesday], we had to really concentrate on getting on both sides of the plate.
Saltalamacchia notes the Marlins aren't looking to become some buzz teams inside club. They need to make pitches to all sides of the plate.
"I'm not going to say we need to establish in," the catcher said. "I think we need to make our pitches. We've made some bad pitches over the last few games where we left pitches over the middle of the plate. We were trying to go away, leaving it over the middle. Try to go in, leaving it over the middle. I'm not going to say we're going to go in on everybody, but we've got to hit both sides of the plate, and we've got to continue to pitch."

subset: commoncrawl


text:
To promote its "adidas is all in" brand campaign, the sporty label has released a video starring Nicki Minaj, Big Sean, Sky Ferreira, Jeremy Scott, Korean pop group 2NE1, and NBA star Derrick Rose. The A-list crew is featured jamming out to Minaj's song, "Masquerade," all over the world in NYC, Tokyo, Paris, and Rio de Janeiro. The ad hopes to inspire customers to represent their own mantras and create videos, which can be submitted into a contest to win autographed gear or MTV VMA tickets, via the brand's Facebook page.
Watch the video and see images from the campaign, below.


text:
Work Begins at School
by James E. CastoPhoto Gallery
Too many of the nation's young people graduate from high school unequipped with the skills they will need to perform in today's modern, highly competitive job market. Often they flounder, ending up in unsatisfying, low-paying, dead-end jobs. At the same time, many employers report they are hard-pressed to find enough new workers who are adequately qualified for today's jobs.
Tackling both these problems—helping both students and employers—is what School-to-Work programs are all about. It's a new approach to learning that's taking hold in more and more of the nation's schools.
"It's based," says Jeff Krauklis, assistant superintendent of Clay County Schools and director of the Clay County School-to-Work Partnership in West Virginia, "on the concept that education works best, and is most useful, when students apply what they learn to real-life, real-work situations."
For Angela Fitzwater, a senior at Clay County High School, that means spending part of each school day at Clay's Primary Care Systems, where she escorts patients, files records, and, with patients' permission, sometimes gets to observe medical procedures such as electrocardiograms. It's a made-to-order assignment for Fitzwater, who says she plans to be a nurse. "This shows you what you've got to do and what you need to do to get there," she says.
Her classmate, Phillip Duffield, also feels he's getting a valuable jump start on his future. Duffield hopes to become a teacher, and so he's now working in the afternoons in a Head Start classroom near the high school campus. He helps the children with art projects and computer programs, and has even developed and taught a lesson plan on reading strategy. Head Start teacher Linda Harrison says Duffield is doing a great job. "I think he will be a wonderful teacher," she says.
Exploring Careers
Job assignments such as these are the logical conclusion of a School-to-Work program that begins in the elementary grades, where students explore careers; continues in grades 6 through 8 with field trips, job shadowing, and computer simulations; and culminates in grades 9 through 12 with students participating in work-based learning experiences.
Devising and operating a successful School-to-Work program in any school system is a challenge, but it's especially so in a county such as Clay, one of the poorest in West Virginia. The county has a low college-going rate. The school system itself is the largest employer in the county, followed by timbering and mining companies. The county has few jobs for adults, much less work-experience opportunities for young people.
Clay County Schools' workforce readiness program was established in 1998 with the help of a grant from the Appalachian Regional Commission (ARC), which enabled the full-time employment of a School-to-Work coordinator. The work done on the program helped the Clay County School-to-Work Partnership win an Urban/Rural Opportunities Grant for Empowerment Zones and Enterprise Communities through the U.S. Departments of Labor and Education in October 1998. The $1.3 million Urban/Rural grant is spread over four years.
"We never could have won the Urban/Rural [grant] without the ARC funding that put everything in motion," says Krauklis. This funding, he says, has enabled the school system to continue its work-site program, which this year has nearly two dozen students placed in a variety of local work settings, including the courthouse, a bank, a supermarket, the health department, and the sheriff's office.
Clay County Schools School-to-Work Coordinator Anita Stephenson explains that students placed at a work site "perform a variety of tasks, such as answering the telephone, assisting the public with questions and providing help with purchases, entering information into a computer, filing papers, running errands, and answering mail. Critical elements of successful work-site placement are trust in the students and their willingness to accept responsibility."
However, the limited number of jobs available for students in the county has prompted the school system to initiate a number of school-based work enterprises, which enable students to get job experience without leaving the campus.
Each year, for example, students enrolled in Clay County High's building trades construction program build a modular house and sell it through nonprofit Clay Mountain Housing to a low-income family in the area. The house is then trucked away, and the money from its sale goes back into the program to buy materials for the next house.
"This is the fourth one that we've built," says carpentry teacher Brian Holcomb of the handsome three-bedroom, one-bath frame house taking shape on the school campus.
Two years ago, when the community received a $150,000 Empowerment Zone/Enterprise Community grant to construct a community fitness center, the lowest bid from a private contractor was $480,000. Leaders instead gave the job to students in the high school's construction program, who built the center at a total cost of $120,000—and gained valuable job experience in the process. Now that the center is open, it's partly staffed by students interested in learning about physical-therapy and fitness-training careers.
Students also built a structure that houses the dressing rooms at the school's football field.
Hands-On Experience
Other Clay County High School students work in the school's Agricultural Science Department. The school built a for-profit greenhouse on campus (initially funded by a state School-to-Work grant and expanded through the Urban/Rural grant) and runs an aqua-culture/aquaponics center in it. Students do all the tasks involved in maintaining the systems and caring for the fish and plants growing there.
There's also a new student-operated school store, christened the Panther Pit, that stocks a variety of merchandise, including sweatshirts, hats, and other items emblazoned with the school's mascot, a snarling panther. Students who operate the store get valuable hands-on experience with retailing. Senior Melissa Koch, who works at the store during the lunch period each day, uses a handheld scanner, just like the ones used by clerks at department and discount stores, to ring up prices. The store sells items at a modest markup, then plows the profit back into the operation, making it self-sufficient.
And there's a student-run catering service, as well, that lets students learn a wide range of skills needed in the restaurant and food-service industry. Under the instruction of family and consumer sciences teacher Mistie McKown, students take classes in the basics of food preparation, table settings, room and seating arrangements, and other related topics, and provide catering services for events at the school and at off-campus sites. The program is based on the National Restaurant Association Educational Foundation's ProStart school-to-career program curriculum. Students who successfully complete the coursework and log 400 hours of food-service preparation can take a National Restaurant Association test and graduate with a certificate that will help them land a job in the food-service industry.
In an unusual public/private venture, students work with a retired art teacher, Jerry Stover, to help publish volumes of local history. The first such compilation was undertaken in the nation's bicentennial year of 1976 as a school project. Subsequent volumes followed, with students writing family histories and other articles, then preparing the text and photographs for publication. When health problems forced Stover to take early retirement, he moved the operation off campus, setting it up as an independent publishing enterprise that serves as a work-site placement for Clay County High students who are interesting in writing or publishing careers.
Stover notes that when Dusty Bird, who spent two years working on the history book project, graduated and went looking for a job, "he took one of his books with him. Showing it, he got a job on the spot." Bird is now working at a Summersville motel as he saves for college, using his writing and publishing skills to help prepare the motel's promotional materials.
Another important part of the Clay County School-to-Work program involves computer simulations. The simulations, says Stephenson, give students "an opportunity to gain workplace processing skills such as teamwork and communication, as well as experience the broad concepts of a particular industry."
The Riverview Hotel program, for example, is a computer-based simulation that introduces students to the world of hotel management by giving them an opportunity to "work" in a hotel located in River City, a medium-sized city facing a typical array of social and economic issues. Students, working in teams of three or four, jointly assume the role of hotel manager. In a series of episodes, the students interact with members of the hotel staff and guests and are faced with making the kind of decisions that are frequently part of a busy workday.
Other simulations place students at work in a bank, a hospital, or a paper company.
Stephenson says students enjoy the computer simulations. Cathy Lambey and Stephanie Bird, both freshmen, confirm that fact and say they'd like to download the hotel simulation so they can take it home to use. (Copyright restrictions prevent that.)
In a related development, the Clay County School-to-Work Partnership and the West Virginia State College Community and Technical College have teamed up to offer college-level classes at Clay County High, thus affording high school students and adult learners alike an opportunity to jump start their college careers and get some credits under their belts. Day, night, and summer classes are offered.
The School-to-Work trend is not without its critics, who argue that it promotes vocational and technical training at the expense of a liberal arts education and that it forces youngsters to focus on a career choice too early. School-to-Work proponents counter that encouraging students to think about what they might like to do for a living, exposing them to practical experiences in those fields, and then letting them know what skills they will need to do those jobs makes school far more relevant and encourages students to study harder. Moreover, proponents say, School-to-Work helps students better understand the connection between learning in school and living productive lives.
James E. Casto is associate editor of The Herald-Dispatch in Huntington, West Virginia.


text:
Featured Products
Trainer's Toolchest is eager to build training solutions for you. We're a leading provider of human resource and diversity training materials to businesses, government agencies, healthcare providers, educational institutions and non-profit organizations.
It's easy to do business with us. We listen to your needs. Our knowledgeable staff has over 35 years of consultative sales experience in the training industry and can save you time in selecting materials that best suit your training objectives.


text:
Aortic stenosis means that your child has a heart valve that is too narrow or is blocked. The aortic valve is 1 of 4 heart valves that keep blood flowing through the heart. The valves make sure blood flows in only one direction. The aortic valve keeps blood flowing from the left ventricle to the aorta. Your child may be born with aortic stenosis (congenital). Or it may develop later (acquired). It occurs more often in boys than in girls.
A normal aortic valve has 3 flaps (leaflets) that act as a one-way door. In aortic stenosis, the valve doesn't work as it should or has an abnormal number of leaflets that don't work correctly. That makes it harder for the leaflets to open and let blood flow from the left ventricle to the aorta.
Aortic stenosis may be mild, moderate, or severe. It depends on how much of the blood is blocked. The condition may get worse over time. It may also occur with other heart problems or conditions.
Moderate to severe aortic stenosis may affect the heart and blood vessels in these ways:
A child can be born with aortic stenosis. This means the aortic valve didn't form as it should before birth. Sometimes this problem is caused by a genetic problem. But most of the time, the cause for this isn't known. In older children, aortic stenosis may occur after an untreated strep infection.
The symptoms of aortic stenosis vary, depending on how old your child is. They also vary by how severe the blockage is. For example, a child with mild aortic stenosis may have few symptoms. Or he or she may not have any symptoms. Symptoms may not show up until adulthood. Or an infant may have trouble feeding and may not gain weight. In severe (critical) aortic stenosis, infants are very ill.
More severe aortic stenosis may cause:
The symptoms of aortic stenosis can be like other health conditions. Make sure your child sees his or her healthcare provider for a diagnosis.
Your child's healthcare provider may have heard a heart murmur when listening to your child's chest with a stethoscope. A heart murmur is an abnormal sound as blood moves through the heart. A heart murmur may mean that your child has a heart defect. Your child's symptoms are also part of figuring out the diagnosis.
Your child may need to see a pediatric cardiologist to confirm the diagnosis. This is a doctor with special training to treat heart defects and other heart problems in children. Your child may also have tests, such as:
Treatment will depend on your child's symptoms, age, and general health. It will also depend on how severe the condition is. If your child doesn't have symptoms or if symptoms are mild, his or her healthcare provider may just watch symptoms closely. This means your child may often have office visits and tests.
The pediatric cardiologist and a cardiothoracic surgeon will figure out whether your child needs an aortic valve procedure. The procedures include:
Before the procedures you might expect the following:
Complications of moderate to severe aortic stenosis include:
Talk with your child's healthcare provider about his or her risk for these problems.
Congenital aortic stenosis can't be prevented. But all newborns are screened for congenital heart disease with pulse oximetry. This is a simple, painless test to check the amount of oxygen in the blood. It's done by placing a small probe on the infant's arm and leg. If the oxygen level is low, it may mean there is a heart defect. More testing and treatment will be done if a problem is found.
Most children who have had an aortic valve repair or replacement live active, healthy lives. Your child's activity levels, appetite, and growth usually return to normal. Your child should get regular follow-up care with a cardiologist throughout his or her life. Your child may also need:
Contact your child's healthcare provider if you notice:
If you child has had a procedure, make sure to follow all instructions from the surgeon. And make sure to keep all follow-up appointments with your child's cardiologist and surgeon.
Tips to help you get the most from a visit to your child's healthcare provider:
Article Last Updated: August 04, 2020
© 2000-2020 StayWell, 780 Township Line Road, Yardley, PA 19067. All rights reserved. This information is not intended as a substitute for professional medical care. Always follow your healthcare professional's instructions.


text:
Video Devotional for "Stop Spreading the News"
Dr. Ray PritchardDr. Ray Pritchard is the president of Keep Believing Ministries, an Internet-based ministry serving Christians in 225 countries. He is the author of 29 books, including Stealth Attack, Fire and Rain, Credo, The ABCs of Christmas, The Healing Power of Forgiveness, An Anchor for the Soul and Why Did This Happen to Me? Ray and Marlene, his wife of 39 years, have three sons - Josh, Mark and Nick, two daughters-in-law- Leah and Vanessa, and four grandchildren - Knox, Eli, Penny and Violet. His hobbies include biking, surfing the Internet, and anything related to the Civil War.
- 2018 Mar 19
Here's the video devotional for "Stop Spreading the News." Let's keep the main thing the main thing. #MiracleRoad
Click here to sign up for the free email sermons.
Read More Dr. Ray Pritchard"Stop Spreading the News" Monday, March 19, 2018


text:
Mt. Cuba Center open for tours in August
By ACL
Mt. Cuba Center in Hockessin, Del., is welcoming visitors in August for the first time.
For the first time, Mt. Cuba Center in Delaware is open in August. An array of native wildflowers and grasses await visitors. Black-eyed Susans, Joe-Pye, and small wood sunflowers attract butterflies, bees, and the unusual hummingbird moth. Warm-season bloomers also flourish in an area designed to show home gardeners a display of easy-care native perennials -- turtle heads, blue stars, asters and more.
During Open Days, visitors can discover prehistoric plants that live in modern-day gardens. Docent-led excursions are included with admission. Open Days continue every Friday and Saturday through Nov. 9, 2013. Hours are 10 a.m. to 4 p.m. Admission is $6 for adults, $4 for ages 6 to 16, free for children 5 and younger. No reservations are required. For more information, call 302-239-4244 or visitwww.mtcubacenter.org.
Mt. Cuba Center is at 3120 Barley Mill Rd., in Hockessin, Del. It is committed to preserving the rich flora of the Appalachian Piedmont Region through horticulture, research, education and conservation. Classes in native plants and naturalistic gardening, conservation and local habitats, and nature-inspired art are offered throughout the year.


text:
"Invisible Ink is a powerful tool for anyone who wants to become a better screenwriter. With elegance and precision, Brian McDonald uses his deep understanding of story and character to pass on essential truths about dramatic writing. Ignore him at your peril." —Jim Taylor (Academy Award?- winning screenwriter of Sideways and Election)
Tuesday, December 28, 2010
The Strength of the Reluctant Hero
7 comments:
- Clint said...
This is coincidentally timely, considering one of the thematic issues with which I've been struggling in my WWII script is the nature of heroism. No one really intends to be a hero; it just works out that way. And some don't seem afraid when acting heroically; they're merely reacting to situations the only way they know how. I'll have to add this into the mix as I continue to let this stuff percolate.
- Kaki Flynn said...
I've read both Invisible Ink and The Golden Theme, books recommended by Ted Mathot (roseandisabel.blogspot.com).
Interesting blog entry:
Turn around. Hold up your cellphone, and take a picture of your bookshelf and post it here.
- Darrell said...
Thank you for posting this.
Thanks, Brian. I'm in the throes of Vogler's The Writer's Journey and the concept of the reluctant hero was throwing me for a bit of a loop. Partly because it seemed to me that the only reason a hero would enter into Campbell's Special World is just because he wants to, simple as that.
You've clarified the idea with this-the hero wants and doesn't want to enter, but the power that makes him enter inspite of his fear is what makes it so compelling.
- Brian McD said...
Thanks guys,
I'm happy to hear that this post resonates. Thanks for letting me know.
I'm still amazed that anyone reads this thing at all.
As for Kaki's request that I take a picture of my bookshelves and post it, I'm not sure it would be that enlightening. But I will think about it.
- Ashley Armstrong said...
I really enjoy reading your posts. I read about half of Vogler's The Writer's Journey for my Writing for Animation class, and plan to finish it.
This really causes me to think about my story that I'm working on and what it is that the characters are afraid of. I have thought of what those frights are, but this made me realize that I need to make those fears even more emphasized in the beginning of the story.
It also causes me to think about what many modern films that I see lack. And even though some of the characters have fears, like Bella from Twilight (I really don't like this franchise) fears Edward separating from her, but this fear doesn't let her grow stronger. Personally I think it makes her regress. So I think it's interesting to realize that a character needs to have a fear that allows him or her to grow stronger.
- Brian McD said...
Thanks Roxyryoko,
Glad you like the posts and I'm always happy to hear that they may help people.
Just a thought, it may help you to stop thinking of these principles of drama and storytelling and patterns that we see in our own life. I have done that for several years now.
When you read about the Campbell/Vogler hero's s journey imagine when you have gone through those steps in real life. We all go through theme from time to time.
For instance imagine moving to a new school as a child. You probably refused this "Call to Adventure". You were gonna miss your friends.
When you did enter the new school you "crossed a threshold". In this new world you must learn the rules of this place, which might be hard at first. You could call this "The road of trials". This is a series of challenges and ordeals.
Even if they don't seem like it at first the hero's journey steps have real life counterparts and you have been through them and will again.
There is always a new experience that you either refuse or at the very least have some bit of trepidation about and when this happens the other steps naturally follow.
There is a reason that Joseph Campbell saw this pattern repeated in cultures all over the world is because it is rooted in the human experience. If you keep this in mind the steps will make more sense.
It will help your own storytelling because you won't treat the steps as paint-by-numbers, but rather as an organic part of the human experience.


text:
|Official Directory
2017 Directory of Services ( 2017-06-09 16:20:05)
The descriptions of services as stated in this directory were obtained from the respective agencies. Although extreme care was taken in the preparation of this Directory, continual changes have made it impossible to eliminate all errors. Agencies listed in the Directory of Community Services -- or desiring to be listed in future editions of the Directory -- are asked to bring any changes, corrections or new program information to the attention of:
County Directory
We express our appreciation to the listed agencies and organizations whose cooperation enabled this publication. We believe it to be a reliable and comprehensive guide to community services and we trust it will prove helpful to you and your staff.
|Last Updated on Friday, 09 June 2017 15:43


text:
The Queen of Daytime TV decrees: soap operas' reign has past.
In the wake of ABC canceling "All My Children" and "One Life to Live," Oprah Winfrey took to YouTube Thursday to explain why viewers shouldn't expect "the soaps" to land on her new network OWN.
Winfrey said fans had "bombarded" her with requests to take action, and she understood "what it feels like to have a show that you love go off the air."
U.S. & World
"I appreciate that you all think that I could save the soaps," Winfrey said in the two-minute video, MSNBC reported.
But she said it was the "bone marrow truth" that the audience for soaps had dried up over the years. Programs went from having "double-digit ratings" when she first began her TV career to lately "not even getting a full, single digit number," Winfrey said.
She said there just weren't enough people at home in the daytime anymore to tune in to the genre.
"Believe me, if there was a dime to be made from them on broadcast television it would still be happening," Winfrey said. "All good things come to an end."
Check out Winfrey's full vid below.


text:
This week, Apple introduced its next generation of iPhones. While the company traditionally drops the price of the previous iPhone model when it announces a new model, this time around Apple had a different strategy: eliminate the iPhone 5 completely and make a new-looking and budget-friendly iPhone 5C.
Andrew Cunningham was in San Francisco for Apple's announcement and brought us a hands on look at the near future in A tale of two iPhones: Hands-on with the iPhone 5S and iPhone 5C (update: video!). In the comments beneath the story, our readers shared some of their first reactions to the new products.
"That 5C case looks like a Lint Collector Supreme (kind of like a Sorcerer Supreme, but with less neatly kept hair)," wrote Demani. Some, like foetusinc, were bored: "I was hopelessly hoping maybe gold was just a tease and there would be actual colors for the 5S. Red/Green/Blue anodize would look awesome if done right. Honestly, I'd seriously consider buying the 5S guts in a 5C case if it were an option—I'm completely bored with colorlessness."
The fingerprint reader really struck a chord for some. "I'm still struck by the fingerprint reader. It's just so natural a thing to see," cooed Happysin. "It almost feels like Apple decided to go with that button on day one of the first iPhone because one day they knew it would become their fingerprint reader. It's perfect." yolnizzle agreed, adding, "For me, the best features on the new device are the fingerprint sensor and the M7 Chip. Can't wait to see the kind of applications developers churn out!"
But the new phones still disappointed others: "3 years in a row without a bump to the 16/32/64 storage tiers (or a reduction in price). Surely flash prices haven't been anywhere near constant?" asked Daniel Smith. jonah agreed. "We're seeing quite a few new apps and games that take up a comparatively huge amount of space. For example, X-Com takes up 3.5+ GB by itself. 16GB looks a lot smaller when you have about 13GB free to start with and start putting a few AAA games on it."
Just a 5 in 5C's clothing
In our analysis of Apple's launches, Editor at large Jacqui Cheng returned to the Ars CMS to give some perspective of the launch of the iPhone 5C. In her article, The Cheap iPhone™ has been calling from inside the house all along, Jacqui writes about the demographic that Apple is targeting and why it chose to split its line of phones at this point in time.
Commenters on this story were split on hating the colors and design of the 5C and hating the price. Some even managed to do both!
"$550/$650 for the 5C off contract is nowhere near cheap," wrote agifford. "'Costly' is far more appropriate. $249 off contract for a Nexus 4 is a screaming deal in comparison." Onerunjuror was not swayed by the price either: "As someone who was seriously interested in the 5C, that unlocked price is just baffling. It needs to be at least $150 cheaper to be considered "cheap" to someone who is very on the fence about buying one. I probably should have expected a price not too much cheaper since it has the guts from an iPhone 5, but that was kind of a shocker to be honest. I want to replace the parents' 3GS's, but at that price it may have to wait. At least I have until Christmas to save the money should I go ahead and get them each one."
grimmReaper pulled no punches, "the iPhone 5C is not a cheap phone. It is still a costly phone made with cheaper material. Usually Apple releases an 'S' model and reduces the price of the previous model to $99 (with contract). Instead they are offering a plastic iPhone with a $99 contract. I don't see how this will help the customers."
"So 'C' is for Cheap, and 'S' is for 'Spensive?" quipped craigc.
RockDaMan was tentatively impressed, however:
Great writeup.
I'm in the market for a new phone—but no way you can buy one of these sight unseen. I'm going to have to see them in person before deciding on model. I'm into future-proofing when I buy a device I plan to use for two to three years, but I might forgo the technical wizardry of the 5S and get a 32GB white 5C. Decisions, decisions...
My unpopular opinion: the gold 5S will find plenty of takers, not just overseas, but in the USA as well.
In reference to RockDaMan's gold 5S comment, glap1922 responded, "I hope you aren't right, but I fear you are."
Nicky G didn't understand all the 5C haters: "I kind of wish I could get 5S guts in a 5C case! I like the plastic and colors! It's freaking fun, you dorks."
But peterford pointed out something that you'll notice if you look carefully at the image of the iPhone 5C back and case above:
iPhone 5C... lovely.
Interesting colors... lovely.
Snap on contrasting colors ... marvelous!
Holes that make the back of your phone say "non"...um, that's a bit crap isn't it?
The final frontier
Although we may have heard this a number of times before, this week scientists announced that they really, truly...well, probably...believed that Voyager 1 has left the Solar System. Matthew Francis explained in Voyager 1 really has left the Solar System…probably that researchers have become more certain than ever that Voyager 1 is a long, long way from home.
arcite is apparently a space probe that dropped in to leave a quick comment: "I feel a kinship with this ship as its pretty much the same as me. Go forth V'ger into the great space between, may you achieve sentience and one day return in search of your maker."
Bron marveled at the mechanical prowess of Voyager 1's creators. "The Voyager probes have always amazed me. 37 years on and still working (mostly)—and now Voyager 1 has likely left the Solar System. Hats off to NASA and their engineers." Shudder, obviously one of our humanoid (read: inferior) readers, could not compare to Voyager 1: "I'm 36.75 and some of my systems have shut down already. This is quite a feat! The vastness of space is so mind-boggling. Here we have something that is so far away we can't make sense of it, and it's the celestial equivalent of me getting 2 inches outside my front door."
But Airborne Geek took the prize for most enthusiastic: "The Voyagers are still the best thing we've ever done," Geek wrote.


text:
Mike Rusczyk is finally putting his creativity to use. Anyone who knows Mike knows that he is one of the most underrated skateboarders out there, in terms or skateboarding ability and artistic creativity. One of Mikes friends is starting a personalized clothing store that sells designer clothing and focuses on tee shirt design, and Mikes helping to design a line for the store. It will give him a chance to have a store front to sell his shirts that feature, in his words, fucked up stitching shit, stuff thats low key and good. He now has a 4-table screen press in his garage, and a sewing machine, so look out for Mike to be wearing some new fresh shit the way he likes it. Been skating and shooting some photos, and nerding out on the compu-trip-flip tripper...
Corey Duffel now rides for the Consolidated blanket team, how fun!
Im hearing a little rumor about Leo Romero going pro, more on those rumblings when theres more to tell.
Over all, the Foundation team is really concentrating on Thats Life. Justin Strubing and Daniel Shimizu just got back from Spain with a ton of amazing footage. Leo and Matt Allen are shutting down spots all over in Europe at this very moment, shooting video footage and also filming for an article in Document. The rest of the team has been filming all day, every day, putting down some film that will become a future legend. Thats Life is tentatively scheduled for a Spring/March 2004 release, but if you cant wait for the premiere, you can see a teaser of the video at the upcoming ASR show in January.


text:
Author: Nicolas Jayr, Team Manager, BBH London
Could your career do with a one-on-one mentoring session from Sir Nigel Bogle?
Or do you have a speech (or resignation letter!) that needs copywrangling by multiple award winning copy legend David Kolbusz?
Or maybe your profile could benefit from an audit from BBH's top social media strategists?
These are just three of the dozens of unique experiences that will be auctioned or offered as lottery prizes as part of the CITYofGOOD project. Other items available include a wine tasting session with BBH founder (and vineyard owner) Sir John Hegarty, a racing top signed by Usain Bolt and a portfolio review from BBH Executive Creative Director Nick Gill.
All money raised goes to support Brazil NGO Grupo Ruas e Pracas and is part of The International Exchange initiative which brings together communication professionals and NGOs working in developing countries.
You've got until 10 October to decide which fantastic experience you want to bid for and make your offer. Follow @bbhcityofgood for updates and good luck!
Nicolas is heading to Recife (North-East Brazil) in November as part of the TIE initiative to work with NGO Grupo Ruas e Pracas, whose mission is to empower children and adolescents living on the streets through an educational process based on street education.


text:
Journal: Journal of Animal Ecology J Anim Ecol Publisher: (9694) Wiley-Blackwell Publishing, Inc. (Alias: John Wiley & Sons, Inc., John Wiley & Sons Ltd., Wiley, Wiley-Blackwell, Wiley Blackwell, J. Wiley & Sons, John Wiley & Sons, Wiley-Blackwell Publishing Ltd., Wiley and Sons, Wiley-Blackwell Publishing Ltd, Wiley-Blackwell Publishing Asia, Wiley-Blackwell Publishing Ltd.) ISSN (P) 0021-8790 ISSN (E) 1365-2656 ISSN (L) 0021-8790There are no additional documents for this Publisher/Journal.
|Item Type:
|Accepted Manuscript:
|Embargo Accepted Manuscript:
|12 months embargo for scientific, technical and medicine titles2 years embargo for humanities and social science titles
|Published PDF:
|Publisher Statement:
|For accepted manuscripts: This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at https://doi.org/[insert DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. (http://www.wileyauthors.com/self-archiving)
|Refereed Source:
|Ulrichsweb ID:
|07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
|Sherpa Romeo ID:
|Sherpa/RoMEO conditions:
Some journals have separate policies, please check with each journal directly
On author's personal website, institutional repositories, arXiv, AgEcon, PhilPapers, PubMed Central, RePEc or Social Science Research Network
Author's pre-print may not be updated with Publisher's Version/PDF
Author's pre-print must acknowledge acceptance for publication
On a non-profit server
Publisher's version/PDF cannot be used
Publisher source must be acknowledged with citation
Must link to publisher version with set statement (see policy)
If OnlineOpen is available, BBSRC, EPSRC, MRC, NERC and STFC authors, may self-archive after 12 months
If OnlineOpen is not available, BBSRC, EPSRC, MRC, NERC and STFC authors, may self-archive after 6 months
If OnlineOpen is available, AHRC and ESRC authors, may self-archive after 24 months
If OnlineOpen is not available, AHRC and ESRC authors, may self-archive after 12 months
Reviewed 18/03/14
Please see former John Wiley & Sons and Blackwell Publishing policies for articles published prior to February 2007
|Article Processing Charge(s):
|Date Deposited:
|22 Oct 2012 17:21
|Last Modified:
|21 Apr 2020 00:49


text:
Sun's VirtualBox virtualization software release offers interesting features that allow developers to measure multicore scalability. As these new features were not available in previous versions, it is very important to consider them when evaluating a virtualization solution in order to test parallel performance in different operating systems and hardware environments.
A not very well-known proverb says "After you eat fruit, thank the tree". A user with a multicore microprocessor should show gratitude to a successful multicore scalability.
Talking about information systems, scalability is an application's ability to upgrade its performance, its response time or the number of users to which it can provide services. We can measure scalability from many points of view (among others):
- Processing capacity.
- Features and services (new functions, new cards, new hardware).
- Storage capacity.
- Memory.
One of the great difficulties related to multicore programming is the possibility of testing the application's scalability using different hardware and diverse operating systems. If you develop an application without multiplatform in mind, you may still have to test it on different operating systems. For example, an application designed to run in Windows XP SP 2 and later should also run on Windows Vista; Windows 2003; Windows 7 and Windows 2008.
Virtualization software really simplified the testing process. You can run many different operating systems in a sandbox, using different memory and storage configurations without the need to install a quad-boot system. Previous versions of VirtualBox offered this capability. However, they had a great problem for developers working with multicore programming, concurrency and parallelized algorithms. They allowed creating virtual machines using just one processor, one physical core, one logical core, just a single thread. Hence, you couldn't really test applications designed to exploit multicore microprocessors using VirtualBox. Besides, other popular desktop virtualization software was limited to just two processors, two physical cores, two logical cores, two concurrent threads. If you worked with a quad-core microprocessor, you wouldn't be able to assign more than two logical cores to the virtual machine.
I liked VirtualBox. It was very easy to install, very easy to configure and it offered exciting features. However, I wasn't able to work with multicore virtual machines. It was impossible to test multicore scalability using VirtualBox.
However, Sun recently launched VirtualBox 3.0. One of the most exciting features is its capability to create virtual machines with up to 32 cores, 32 concurrent threads. Really awesome! Nevertheless, you must be careful and you must consider many restrictions when working with virtualization to test multicore scalability.
The results are not going to represent the strict results offered by the equivalent real, physical hardware configuration. However, you will get a rough idea of what's going to happen in a real hardware configuration similar to the one being virtualized.
For example, if you want to test the multicore scalability for a parallelized application in Windows, you can change its process affinity settings. A few months ago, I However, this technique is just restricting the logical cores that the operating system is going to use to schedule threads created in this process. The other concurrent threads running on the operating system, belonging to dozens of concurrent processes, are going to take full advantage of all the available logical cores. Thus, this technique will not offer an accurate result. Sometimes, an application uses other services. These services could also be trying to exploit multicore capabilities. In these cases, it is even more important to use other techniques.
You can change some Windows settings to restrict the number of logical cores in the boot sequence. This technique could offer more realistic results. However, you should need to reboot as many times as the different number of configurations that you want to test. If you want to test the application running with 1; 2; 3; 4; 5; 6; 7 and 8 logical cores, provided that you have an Intel Core i7 or similar microprocessor, you will have to reboot 8 times. Believe me, rebooting 8 times is a very frustrating experience.
Using VirtualBox 3.0, you have two alternatives to simplify testing multicore scalability. If you have enough disk space, you can create 8 virtual machines with 8 different configurations (1; 2; 3; 4; 5; 6; 7 and 8 logical cores). If you do not have enough disk space, you can create one or more virtual machines and reboot them changing the number of cores. Rebooting a virtual machine is really faster than rebooting your computer. Besides, you can go on reading your e-mail, talking by instant messaging or using Twitter.
VirtualBox 3.0 offers the possibility to create more virtual cores than the number of available logical cores in the host computer (the one running VirtualBox). For example, you can create a virtual machine with 4 cores running VirtualBox on a dual-core microprocessor without Hyper-Threading capabilities (just 2 logical cores). This feature is a bit dangerous when testing multicore scalability. If you want the most accurate results, you should not configure a virtual machine with more logical cores than the ones available in the underlying hardware. Why? Because you are testing scalability and you don't want to emulate hardware that does not exist. You just want to restrict the number of available cores in certain configurations.
Virtualization simplifies multicore scalability testing. However, you should use a microprocessor with hardware virtualization capabilities to obtain the best results. Besides, if you want to test your applications with many different memory configurations, you'll be happy using a 64-bits host operating system. VirtualBox 3.0 can run on 64-bits operating systems. Thus, you have access to huge memory for your virtual machines. In the forthcoming months, more desktop, notebooks and laptops will have more than 4 GB. It is very important that the parallelized applications consider the available memory in 64-bits systems. You can also test this situations using VirtualBox or similar virtualization software.
Virtualization software is an excellent alternative to test your applications in different potential hardware and software configurations. You can play with the configurations without worrying about damaging your workstation. The results will differ from real hardware configurations. However, you'll be able to know whether your algorithms are scaling as the number of cores increase.
You can find more information about VirtualBox 3.0 new capabilities in this article by Eric J. Bruno.
You can download VirtualBox 3.0 here.
I borrowed the aforementioned definition of scalability from my book C# 2008 and 2005 Threaded Programming: Beginner's Guide.


text:
Jennifer Aniston silenced rumors of a split from Justin Theroux by turning out to support her fiancé at the launch of his new TV show.
The Hollywood couple became engaged in 2012, but two years later, they have yet to show any signs they are planning to walk down the aisle.
Gossip about a possible split recently surfaced as the stars have not been seen out in public together for several months, but they dismissed the speculation at the premiere of Theroux's new TV project "The Leftovers."
The couple walked the red carpet together at the launch event in New York City on Monday night, while they also put on a public display of affection in front of the cameras, hugging and holding hands.


text:
Create an Account - Increase your productivity, customize your experience, and engage in information you care about.
The Inspections Department is a division within the Community Services Department. Duties that fall under the Inspections Department include the following:
The Building Code Inspection program inspects all new or remodeled buildings to ensure they meet state building code. This includes plan review, permit issuance, inspection of construction phases and issuance of certificate of occupancy.
The Fire Code Inspection program inspects existing buildings periodically to ensure they are maintained in accordance with the safety standards of the state fire code. A percentage of commercial and large multiple residential buildings are inspected yearly.
The Heating and Plumbing Code Inspection program inspects new and remodeled buildings for heating and plumbing compliance.
The Housing Inspection program inspects all existing buildings periodically to ensure they are maintained within the city housing code. Rental properties are inspected a minimum of once every three years and homes being sold are inspected as part of the truth-in-housing program.
Environmental Health inspections are contracted out to a health inspector to make regular inspections of restaurants, food establishments and public pools.


text:
Secondary Suite
More and more people are looking to add a secondary suite to their basement or above a detached garage to create additional revenue. Depending on the zoning and associated regulations, you may be allowed to incorporate a suite on your property. However, most existing properties will require modifications to incorporate the requirements of a secondary suite. Developing a new secondary suite or legalizing an existing suite requires a development permit and a building permit.
Why choose Permit Masters?
We are confident that Permit Masters is the best company to assist you with your design and permit process. Our confidence comes from the successful completion of thousands of projects just like yours. Here is why:
We make it simple
The design, permitting and construction process can be complex and time consuming. Years of dedication and hard work have allowed us to make the design and permitting process simple for our client.
We are a reputable company
Our reputation for providing quality services and sound advice precedes us. One project at a time, we have built our reputation within the industry and with various municipalities. We have even provided successful advice to municipalities looking to improve their own approval process.
We have a proven track record
With over 3,000 home improvements projects, we have built an unmatched knowledge base that can provide you with the support you need to design and plan your project.
We have an experienced and professional team
All our project managers are qualified professionals. Our dedicated team of architectural technologists, designers and urban planners have all helped hundreds of clients achieve their goals.
We have great design expertise
Not surprising that with extensive experience comes great skills. Our project managers can guide you through the design process with confidence and ensure that the design outcome meets all the regulations applicable to your project.
You will have one point of contact
Once your project starts, you will be assigned a project manager with the proper qualifications and experience. This one point of contact will take you through the process one step at a time.
We're flexible and cost-effective
Because we are a small company with a dozen project managers, we're also responsive, flexible and agile. We can adapt and respond to your needs with the speed, efficiency and cost-effectiveness that other companies just can't match.
We have a time conscientious team
Not only can you expect phone calls and emails returned quickly, but your project manager will also keep you updated with each step of the process. We also know that quality drawings and a complete permit submission package will get you to the finish line more effectively.
We have an excellent success rate
In fact, our success rate is above 99%. When reviewing a new project, we identify the potential issues and provide you with alternatives and solutions. We always have a fair approach with our clients; if we don't think something is achievable, we will not drag you into a long costly process.
What is a Building Permit?
A building permit is one that allows the construction of a building or structure to move forward on the condition that it addresses and is in compliance with the building code of a city, town or county. A building permit is necessary when any construction, relocation, alteration, demolition or repair is taking place on a property. In some instance, some buildings and structures are exempt, such as...Read more >
What is a Development Permit?
A development permit provides permission for a specific type of development to take place on a site. The permit can be for any development such as the construction of a new house or the change of use of a commercial space from a retail use to a restaurant. The review process ensures that the proposed development meets the regulation of the local Land Use Bylaw (Zoning). For example, the developmen...Read more >
We're here to help with all your development and construction project permits:
Commercial Permit


text:
In my last post, I considered a question of Catholic identity, stemming from a story about the Diocese of Arlington, and their request that all catechists make a profession of faith.
A second item in the press has also raised the question of Catholic identity. In the New York Times, Ross Douthat, one of the most perceptive observers of modern religious trends, wrote on the question "Can Liberal Christianity Be Saved?". The focus is on the Episcopal Church, but the piece (and his recent book, "Bad Religion") is also a challenge to us as Catholics to consider the identity of our own Church.
"Liberal Christianity" is a notoriously protean entity, but it can be found in every Christian community, including the Church. It has variously been known under the term "modernism", or "revisionism". It is often conflated with political liberalism, but the two are not always or necessarily connected or identical. Theological liberalism's characteristics include:
Those of us raised in the 1960's and 1970's are very familiar with this brand of "liberal Catholicism". We have all been immersed in it, and have seen its failure, which led Cardinal George to call it "an exhausted project… [that] no longer gives life". There are many, many flaws in liberal Christianity, and Cardinal George does an excellent job of dissecting the corpse.
Douthat's piece in the Times focuses our attention particularly on the failure of liberal Christianity — and liberal Catholicism in particular — to properly understand the nature and purpose of the Church. In that view, the Church is merely another sociological phenomenon, no different from any other worldly entity, the purpose of which is limited to worldly matters — to empower people (women, minorities, etc.), redress historical grievances, effect political change, and so on.
This fails to understand the nature of the Church. She is the Body of Christ, His Bride, and is, in a deep existential sense, inseparable from Him. Although made up of flawed and imperfect humans, we can never speak of the Church without speaking of Christ Himself. She is human, indeed, but She is also divine. As both a human and divine entity, the Church respects both the human and divine aspects of every person.
So, the purpose of the Church is also not limited to human affairs. Her ultimate purpose is to bring people into a loving relationship with Jesus Christ — an encounter with a real person — so that people can come to know the Father through the Spirit, and thus attain eternal life. While the earthly activities of the Church are valuable and must be pursued out of obedience to the will of God, they all take a distant second place to that fundamental task of bringing people to God.
Liberal Christianity doesn't think of the Church that way, and never speaks of Her that way. That is why, as Cardinal George pointed out, it "no longer gives life". Indeed, that is why liberal Christianity is diminishing in numbers and influence, because their interests (politics, sexual innovation, environmentalism, etc.) do not appeal to the basic desire of people to know and love God. As evidenced by its obsession with separating sexuality from fertility, liberal Christianity is sterile, and we all know where sterility leads.
The real Church, which passionately loves Her devoted Bridegroom, longs to bring everyone to know Him as well. She is focused on the final goal — life forever in the eternal exchange of love that is God's own life.
Our real Church is rich and fecund, and will always bear fruit.
Tags: Liberal Christianity


text:
Alameda Point
Business owners who are frustrated about the Point's bursting water mains, potholed roads, overflowing sewers and spotty phone and electric service have urged the City Council to approve a 68-acre waterfront development proposal there known as Site A, which they believe will start to fix the problems with an investment of more than $100 million in new infrastructure. The council is expected to vote on whether to move forward with development of Site A on June 16.
How much longer will your commute be if 1,425 homes are built at Alameda Point? That's the question residents who worry about the traffic development at the Point and elsewhere on the Island will create are asking.
Development both on and off the Island will create a lot of traffic on Alameda's major roads and in the Webster and Posey tubes, the city's planners and studies done for local development proposals say – impacts that some fear will overwhelm the Island's arterials, creating commute hour carmageddon. But quantifying those impacts – effectively, predicting the future – is a more elusive matter than the studies let on.
During the final moments of this past Monday's Planning Board meeting, City Planner Andrew Thomas announced a plan that would cap the number of homes that can be built on 37 acres of the North Housing parcel – Navy land that sits just north of the housing now occupied by members of the Coast Guard.
A few weeks ago, I had an interesting e-mail exchange with a friend regarding the redevelopment of Alameda Point. While the conversation about the Point at City Hall seems to revolve chiefly around whether or not to build homes at the Point and what kind of traffic those homes might generate, my friend offered a more nuanced view of the pros and cons of proposed development on the former Naval Air Station.
Development of Site A (or any portion of Alameda Point) can't proceed unless four of the council's five members agree to move forward. So if two council members have already said they oppose building homes at Alameda Point, what are the chances that the development will move forward?
When the federal government shut Naval Air Station Alameda's doors, in 1997, the prevailing wisdom was that cleanup of the toxics the Navy left behind would be complete in a few years, for less than $100 million. Eighteen years and more than a half billion dollars later, the cleanup team working to clear and contain contamination at the federal Superfund site.
Dating back to the last two centuries, Alameda's history has included farmers who worked the land. At Ploughshares Nursery, that history has come full circle.
The City Council voted Wednesday to approve an exclusive agreement with Alameda Point Partners to negotiate a deal to develop a 68-acre waterfront town center at the former Naval Air Station, despite opposition from residents who think the lame duck council should wait and let the new council decide.
"This is a tough decision. But stalling is not the answer to a tough decision," Vice Mayor Marilyn Ezzy Ashcraft said.
City staffers have selected a finalist in the contest to develop a waterfront town center at Alameda Point.
The Department of Veterans Affairs officially took ownership of 624 acres of Alameda Point on Monday, which it hopes to transform into a new, one-stop medical and benefits center, a national cemetery and a wildlife preserve.
"We are proud that the new One VA facility will call Alameda home," Mayor Marie Gilmore said during a public ceremony at the Alameda Theatre & Cineplex that included a color guard, speeches and a video offering the history of the former Naval Air Station and renderings of the planned facilities.


text:
The parade will go on. The parades will go on. At City Hall today Mayor Michael Nutter accepted $200,000 from the new Greater Philadelphia Traditions Fund and announced another $100,000 will be received. The money will cover non-police parade costs for the Mummers Parade and 9 other parades the past two years and going forward through 2012. Congressman Bob Brady and philanthropist Gerry Lenfest and others were thanked for their creation and contribution to the fund. The fund provides a stable source of dedicated revenue and the arrangement gives the community organizations a better sense of what the costs will be. Freedom in 21st Century Philadelphia is not free, but it now appears to be more affordable.
Filed under: Mummers | 1 Comment »


text:
Email: firstname.lastname@example.com
Email Tel:+220 3726339 or Whatsapp +31647366151
Chilling out in beautiful surroundings right next to a fantastic beach is our speciality.
Our wonderful and unspoiled location and relaxed atmosphere makes Boboi the ideal
place for a holiday. Our accommodation is comfortable and clean, our food is great
and our beer is cold...
Get to know the people, places, history and legends of Kartong for an experience
to last you a lifetime. Our staff are your hosts; each with a pride and passion for
delivering a great experience.
Welcome to our personalised activities and tours. Touring with Boboi means more personal
attention, more time with the locals and more time discovering this unique place.
Thanks to Joe Lang for his great photos


text:
You are currently browsing the tag archive for the 'SheevaPlug' tag.
Tag Archive
New Webcast: Java SE Embedded Development Made Easy
06/06/2011 in Mobile & Embedded | Tags: Apache Tomcat, Embedded Java, Java SE Embedded, Java SE for Embedded, JMX, NetBeans, SheevaPlug, webcast | 4 comments
A couple of days ago I blogged about the release of a new embedded Java product called "Oracle Java ME Embedded Client". The interest has been tremendous, and I plan to follow up with more information and a webinar on this topic.
In the meantime, I would like to point your attention to another embedded Java product: Java SE for Embedded. Unlike the Oracle Java ME Embedded Client, which is a Java ME (CDC)-based runtime, the Java SE for Embedded product is a full-blown Java SE 6 runtime, optimized for embedded use in a number of important ways (footprint, memory, power, platform support, and more).
My colleague, Jim Conners, has created an excellent webcast called "Java SE Embedded Development Made Easy". It consists of two 15-minute video with slides and demos covering all the basics of embedded Java SE development:
- Embedded Microprocessor Trends, covering ARM, Intel, and Freescale
- Getting Started with the SheevaPlug
- The Java SE Embedded Runtime Environment: Downloading and Installing
- Running Apache Tomcat on the SheevaPlug
- Installing and Setting Up NetBeans for Embedded Java Development
- Running the ScoreBoard Application locally and on the SheevaPlug
- Live, Remote Debugging of the ScoreBoard Application
- Monitoring the Remote ScoreBoard Application using JMX and JConsole
Once you're on Jim's blog you should also check out his entry "The Unofficial Java SE Embedded SDK", which talks about using your favorite desktop Java IDE for embedded Java development.
— Terrence


text:
£150m will be removed every year from the Scottish economy as a result of the UK Government's new Housing Benefit law. More than 95,000 households in the social rented sector will be affected by the reform and this will mean an average monthly loss of up to £65 for claimant tenants.
Great concern has been raised among Scottish citizens and the Scottish Housing Minister Keith Brown expressed his discontent about this measure. Speaking ahead of a debate on the UK government's Welfare Reform Act on 21 March 2012, Mr. Brown stated:
"It is the responsibility of the UK Government's Department for Work and Pensions to ensure that the welfare reforms are successfully rolled out and – even though we disagree with the changes – the public sector in Scotland must work with them to ensure no one suffers undue hardship'.
He believes changes to Housing Benefit will have a "huge impact on local communities and individuals, some of the most vulnerable ones in Scotland". In order to tackle the impacts, the Scottish Government and COSLA have established a Housing Benefit Stakeholder Advisory Group to help those affected ones properly understand what the impact of the UK Government's changes to housing benefit will exactly involve.


text:
Wake up. Come out to see how beautiful the day is.
Run under the sunlight and try to get me over the hills.
Put the biggest smile on your face and open your arms running round and round.
Lie on the grass with me. Blue sky is over, so let's stare in wonder.
Don't bother if you see grey clouds and it's starts to pour silver rain over you sometimes.
'Cause after the storm you'll see the rainbow. And maybe butterflies and bees come around you to prove all around us is silver and gold today.


text:
Using Multiple JavaScript Onload Functions
JavaScript is one of the most popular programming languages in use on the World Wide Web. It can create interactive pages through the use of menus, forms and calendars. It can also be used to track a visitor's history on your site, play games and many other things.
When scripts are written they're used to accomplish a given task, e.g., create a rotating picture gallery, or to validate a form. This means that for each different type of usage, a separate script is necessary. Often, a script is called using an
onload function.
Loading the Page
When a Web page is loaded, the code on the page — whether HTML, CSS, PHP, or some other type — is generally processed from the top down. As the browser comes upon a section of code, it's executed and the intended action is (hopefully) performed. Let's say you have the following script in the
head section of a Web page:
When the page is loaded, the browser will stop at the script above and execute it. An alert window will pop up and the page will stop loading until the alert window is closed. Often, this isn't an issue, but if the script needs to access elements on the page that aren't yet loaded (i.e., below the current code), you will encounter problems. To resolve these, here are a few solutions.
Move it to the Bottom
One method is to place the script at the bottom of the Web page, just above the closing
</body> tag. This does, however, present a few problems. First, there is a period of time that those elements which are you seeking to change with the script will be clearly seen by the visitor. Then, once the script is executed, they will "magically" change. With a fast loading page and a script that executes quickly, this might not be a problem, but if the original elements can be seen by the visitor, and are then changes, the visitor may perceive it to be some type of a hack or bug on the page, and leave the site immediately. Obviously, that's not good.
Secondly, either the script, or a link to it, will need to be included in the actual HTML
body code. That's not unobtrusive JavaScript. It's best if you can keep everything separate. You'll find it's easier to work on any aspect of the page, and the page itself will load faster.
onload Function
window object in JavaScript has an event handler called
onload. When this event handler is used, the entire page and all of its related files and components are loaded before the function listed in the
onload event handler is executed, hence the term "on load."
As shown in the script above, it's easy to use, but what if you have more than one function you want to call using an
onload event handler?
You would think you could just stack them like this:
Unfortunately, it's not that simple. After the first
onload event handler is executed, it will be replaced when the second
onload event handler is executed. That, in turn, will be replaced immediately just as soon as the third
onload event handler is executed. There are workarounds for this, though.
Let's Put Them in a Chain
One method that has been used quite a bit is the linking of multiple events. It places several function calls in a chain, using one
onload event handler. The method would look like this:
Still, once again we run into the unobtrusive problem. In addition, you will need to add this to the
<body> tag of every page that needs to use the called functions. That could be quite tedious for a large Web site, especially when changes or additions are needed.
Within Another Function
Another method is the following script:
While this is good, there's one method that's even better.
addLoadEvent Function
This function was written by Simon Willison. It has several advantages. For one, it's really unobtrusive. It can be placed in a file with your other scripts or in a separate file. (If it's in a separate file, just be sure to call it after the other files, so the functions will be available.)
Also, it works even if the
onload event handler has been previously assigned. Simon explains it like this:
"The way this works is relatively simple: if
window.onloadhas not already been assigned a function, the function passed to
addLoadEventis simply assigned to
window.onload. If
window.onloadhas already been set, a brand new function is created which first calls the original
onloadhandler, then calls the new handler afterwards."
Isn't that beautiful? So, if you already have a script that uses the
onload event handler, you don't need to dig it out and change it, unless you want to. It also allows for extra code. Here's an example that calls two functions and adds a third, independent
After calling
func1 and
func2, a function is created that changes the background color of the Web page. Each of these is executed in the order shown. Try it yourself and see how it works!
Sometimes, figuring out which approach to use in coding takes a bit of trial and error. Other times it's advantageous to search the Web and see what others have done. The
addLoadEvent function should work just fine in this situation. We use it over at the JavaScript Source for all of our scripts requiring the
onload event handler.


text:
Art, the creation of artful objects, and the appreciation of beautiful things, has always been extremely important to our family. Bead embroidery is the natural progression from painting to fabric to painting with beads on fabric.
Moggie has a degree in Textiles and Clothing. Embellishing with beads adds the sparkle she loves. Meg has a degree in Behavioral Science. She just loves that the beads behave! Both are mothers, daughters, and teachers. Both love to start projects (do they ever finish any.) Most of all, they both love to stitch beads on anything and everything!
This mother-daughter team teaches bead embroidery classes and sells beading supplies at The Innovative Beads Expo and other shows in the Northeast. They also host the Beautiful Bead Embroidery Retreat in Rehoboth, DE.


text:
Have an account?
Need an account?Create an account
Join us at Hannover Messe, April 23 to 27, 2018
Hall 6.0, Stand G30
Cisco Industrial Ethernet 2000 Series Switches extend enterprise technology to industry.
Major sectors of the economy are undergoing a transformation as robots, vehicles, and industrial control systems connect to the Internet. This trend is enabling unprecedented levels of automation, not just at the factory level, but throughout business processes. Cisco can help customers realize profound productivity benefits by transitioning operational technology to standard Ethernet and IT.
Cisco is uniquely positioned to address these new demands on industrial networks, which require a greater need for improved inter-connectivity across industrial equipment and enterprise networks. For example, the Cisco IE 2000 Series Switches provide consistent network services between industrial networks and enterprise business applications. They also provide integrated security and better manageability, creating a truly intelligent network.
Cisco Industrial Smart Solution helps manufacturers boost operational intelligence.
Learn how the next evolution of the Internet is changing everything.
The industrial sectors are promoting the explosion in the number of machine-to-machine wireless connections. The Cisco Visual Networking Index (VNI) estimates two billion connections by 2016, demonstrating the need to more tightly connect and integrate devices, machines, and vehicles with traditional enterprise networks. How will your enterprise make the transition?
The following products are at the forefront of that evolution:


text:
Goodgame Big Farm
Goodgame Big Farm
Goodgame Big Farm
Uncle George has left you his farm, but unfortunately it's in pretty bad shape. Using business skills and the help of your neighbors, family and friends you can turn the overgrown barnyard into a beautiful and prosperous farm again. Plant the right crops, harvest and process them, care for your animals and make sure that your workers are happy – this is how your farm will grow successfully.
Other strategy games
Multiplayer games
Goodgame Big Farm - Google+
Goodgame Big Farm - Facebook
Free Online Strategy Game
Car Games
Goodgame Big Farm , simulation game
www.goodgamebigfarm.co ©2012-2017
Calcula los usuarios online de tu web o blog
PageRank Google


text:
Hi All,
What about an alternative to the new AppAnalytics released with XE8?
I've developed a "Customer Use Analytics" for my applications using a Google Analytics account and a TWebBrowser inserted in my Application.
It have been working since May/2013, so it is not a plagiarism!
Please let me know if this idea sounds good to the BCB Journal's community.


text:
From Obama's 60 Minutes interview, this seemingly obvious but all-too-rare, refreshingly non-ideological declaration about how best to govern:
We've gotta come up with solutions that are true to our times and true to this moment. And that's gonna be our job. I think the basic principle that government has a role to play in kick starting an economy that has ground to a halt is sound.
I think our basic principle that this is a free market system and that that has worked for us, that it creates innovation and risk taking, I think that's a principle that we've gotta hold to as well. But what I don't wanna do is get bottled up in a lot of ideology and is this conservative or liberal. My interest is finding something that works.
And whether it's coming from FDR or it's coming from Ronald Reagan, if the idea is right for the times then we're gonna apply it. And things that don't work we're gonna get rid of.
Talk about a welcome change from the ideology-first recent past.


text:
GAFFE-TASTIC JAGMEET: Singh bungles up Venezuela file again!
WATCH ABOVE as Editor-in-Chief Adrienne Batra and principal at Navigator Amanda Galbraith discuss the latest political gaffe, this time NDP Leader Jagmeet Singh, who is giving our Prime Minister Justin Trudeau a run in the malfunction department.
What do you think about the ongoing political turmoil in Venezuela?
Tweet and Facebook us!
And don't forget to subscribe to our YouTube Channel.


text:
The Food and Drug Administration (FDA) has added a "black box" warning for fluoroquinolones, a type of antibiotic that includes Cipro (ciprofloxacin) and Levaquin (levafloxacin). The warning refers to an increased risk of tendon problems, including tendinitis and ruptures that have been reported in people taking these drugs.
Cipro, the most widely prescribed fluoroquinolone, is used to treat many bacterial infections. In HIV, it is often used with other drugs to treat MAC (Mycobacterium avium complex) and drug-resistant TB (tuberculosis).
People taking any fluoroquinolone who experiences tendon pain or swelling are recommended to stop taking the drug and seek medical care.
Black Box warnings are the FDA's most important notices on potential dangers of a drug. They are set apart from other safety information to draw the attention of prescribers and patients.
Read more information about MAC.
Read more information about TB.


text:
In a parallel-world 21st-century San Francisco where the Kaiser's Germany won World War One and went on to dominate the world, Paul Gomes and his father Lawrence are secret agents for our timeline, posing as traders from a foreign land. They run a storefront shop called Curious Notions, selling what is in our world routine consumer technology-record players, radios, cassette decks--all of which is better than anything in this world, but only by a bit. Their real job is to obtain raw materials for our timeline. Just as importantly, they must guard the secret of Crosstime Traffic--for of the millions of parallel timelines, this is one of the few advanced enough to use that secret against us. Now, however, the German occupation police are harassing them. They want to know where they're getting their mysterious goods. Under pressure, Paul and Lawrence hint that their supplies comes from San Francisco's Chinese....setting in motion a chain of intrigues that will put the entire enterprise of Crosstime Traffic at deadly risk.Turtledove, Harry is the author of 'Curious Notions Crosstime Traffic', published 2005 under ISBN 9780765346100 and ISBN 0765346109.[read more]
You can find lots of answers to common customer questions in our FAQs
View a detailed breakdown of our shipping prices
Learn about our return policy
Still need help? Feel free to contact us
View college textbooks by subject
and top textbooks for college
With our dedicated customer support team, 30-day no-questions-asked return policy, and our price match guarantee, you can rest easy knowing that we're doing everything we can to save you time, money, and stress.


text:
Please contact us if you have any question:
Verena email@example.com
European leader in intelligent climate control tado° raises €10m to fuel global expansion
Munich, July 16, 2014 - The smart thermostat company tado° has closed a new round of financing, raising €10m ($13.6m) from current backers Target Partners, Shortcut Ventures, and other investors.
tado° is the European market leader in intelligent climate control for consumers. The tado° products enable households to save significantly on energy costs while also reaching a higher level of comfort. Heating or AC systems with tado° automatically adjust to users' needs with a geo-aware app for presence detection. The tado° smartphone app automatically recognizes when residents are leaving or approaching home and sets the temperature accordingly. Additionally, weather forecasts, housing characteristics and smart algorithms complete tado°'s control strategy, which can achieve up to 31% in energy savings.
"With this new investment we plan to expand sales of our products to all major European countries and to continue building a global brand. We are very proud of having a great group of investors on board and we appreciate their trust in our company. Target Partners and Shortcut Ventures have made very valuable contributions to help build our company over the last few years," says Christian Deilmann, founder and CEO of tado°.
"Building a fully scalable Internet of Things technology platform and at the same time developing control interfaces for over 5,000 different types of heating systems has been a challenge over the last three years. We are proud of what we have achieved with our team and we are fully committed to creating great products and cutting-edge technology. The new investment will further strengthen our position and allow us to maintain the huge technological head start we have gained in covering Europe's varying heating landscape." says Johannes Schwarz, founder and CTO of tado°. The company employs 40 people, two thirds of whom are software developers.
The first product, tado° Heating, was launched in Germany, Austria and Switzerland in November 2012. One year later the company entered the UK market and this was followed by a worldwide launch of the new tado° Smart AC Control product for air conditioning. Currently tado° products are available through retail partners, utility companies and on www.tado.com.
High-resolution images are available here: https://www.tado.com/dk/press
About tado°
Headquartered in Munich, tado°, the European market leader in intelligent home climate control solutions, was founded in 2011. tado° revolutionises the way energy is consumed at home. Through the use of a geo-aware app, tado° automatically adjusts the temperature based on the residents' locations, enabling households to significantly save on energy costs while reaching a higher level of comfort.
Press Contact
tado GmbH
Paul Hughes
Sapporobogen 6-8
80637 Munich
+49 89 412 09569
About Target Partners
With €230 million under management, Target Partners is one of the leading early-stage venture capital firms in Germany. Target Partners invests in new start-up and early-stage companies, and supports them with venture capital during their build-out and expansion phases. With many years of experience as managers, entrepreneurs and venture capitalists, the team at Target Partners supports entrepreneurs in developing and marketing products and services, building organizations, raising money and taking companies public in Europe and the United States.
For more details: www.targetpartners.de, follow us on Twitter: twitter.com/targetpartners or become a friend on Facebook: facebook.com/targetpartners.
Press contact Target Partners:
Sprachperlen GmbH
Agency for enterprise communication
Helena Pröpster
Ehrengutstraße 5
80469 Munich, Germany
Tel.: +49 89 411 53 199
Fax: +49 89 411 53 200
Email: firstname.lastname@example.com
About Shortcut Ventures
Shortcut Ventures is the venture capital company by founders for founders, which helps start-ups get onto the fast lane without any detours. The investment strategy of Shortcut Ventures focuses on international start-ups in the Internet, mobile, and media sectors. In addition to financial support, the companies benefit from direct access to the extremely valuable founder know-how. Shortcut Ventures is managed by the successful founders and entrepreneurs Dirk Freise, Martin Ostermayer and Thorsten Rehling (such as for blau.de, handy.de). Their portfolio includes the start-ups Mediakraft Networks, SumUp, tado and yuilop. Further information at: www.shortcut.vc.
Press contact Shortcut Ventures:
Felix Altmann
Kattunbleiche 35
22041 Hamburg
Fon: +49 (040) 67 94 46-66
Fax: +49 (040) 67 94 46-11
Email: firstname.lastname@example.com


text:
From OpenWetWareJump to navigationJump to search
Universal Probe Library Assay
Based on the Universal ProbeLibrary Assay Quick Guide from Roche
Workflow Overview
- Planning: Design your primers
- Planning: Design your reactions
- Wet bench: Make Gene Target master mixes
- Wet bench: Make Template (cDNA) master mixes separately
- Wet bench: Add the appropriate combinations of of the master mixes to the 96-well plate
---> Run the reaction in the Light Cycler!
1. Design your primers
- Each gene you analyze requires a set of three oligos: forward primer, reverse primer, and a UPL probe.
- Use Roche's Assay Design Center to design optimal primers, and to identify the right UPL probe for your gene(s) of interest.
- The forward and reverse primers need to be ordered from a DNA synthesis company (e.g., IDT DNA, Promega, etc.), and the UPL oligo comes from Roche.
2. Set up a reaction list and plan the plate layout
- How many reactions should I plan to run? Each experimental cDNA sample is a Template. The gene being detected is often referred to as a Gene Target. You should also include a reference Gene Target such as GAPD (a housekeeping gene that is always active, not expected to change). Each unique template and target combination requires its own reaction. You will also need to set up a no template control to observe the amount of background noise from that reaction.
- Hypothetical example:
- cDNA samples + a no template control = 3
- Gene targets + GAPD reference = 5
- Replicates per reaction = 3
- Wells needed = 3 * 5 * 3 = 45
- A single plate contains 96 wells. To insure accuracy, three technical replicates per reaction (Rxn) are required
- If you need more than 96 wells, you must split the experiment over multiple plates.
- It is absolutely critical that you keep a reaction list and plate layout in your notes. Your plate set-up will probably vary for each run.
3. Reaction set-up: PCR master mixes for each Gene Target
- Label one 1.5 mL tube per gene target
- Make enough PCR master mix for your plate...
- MPK14 is in Reactions 1, 6, and 11 = 3
- Replicates per reaction = 3
- Master mix amount = 3 * 3 + 1 (to allow for pipetting error) = 10
- The same needs to be done for CBX8, TNF, NPPA, and GAPD in separate tubes.
|(Single well)
|Gene Target (x10)
|GAPD (x10)
|2x LC480 Probes Master
|(7.5 μL)
|20 μM Forward primer
|(0.3 μL)
|3.0 GAPD primers*
|20 μM Reverse primer
|(0.3 μL)
|10 μM UPL probe
|(0.3 μL)
|3.0 GAPD UPL probe*
|(0.1 μL)
|Total vol.
|(8.5 μL)
*GAPD primer mix and the GAPD UPL probe are supplied in the Roche Universal ProbeLibrary Human GAPD Assay kit, #05190541001
Resulting 1.5 mL tubes:
- MPK14 - 85.0 μL
- CBX8 - 85.0 μL
- TNF - 85.0 μL
- NPPA - 85.0 μL
- GAPD - 85.0 μL
4. Reaction set-up: master mixes for each Template
- Typically, you will have only 20 μL of stock cDNA on hand.
- Make a 1:10 dilution of cDNA by adding 10 μL of the stock cDNA to 90 μL of PCR H2O.
- Make enough Template master mix for your plate...
- Treated cells cDNA is in Reactions 1, 2, 3, 4 and 5 = 5
- Replicates per reaction = 3
- Master mix amount = 5 * 3 + 1 (to allow for pipetting error) = 16
- The same needs to be done for templates "untreated cells" and "no template" in separate tubes.
|(Single well)
|cDNA Template (x16)
|1:10 cDNA dilution
|(2.0 μL)
|(4.5 μL)
|Total vol.
|(6.5 μL)
*For the no template control, use PCR H2O instead of cDNA.
Resulting 1.5 mL tubes:
- T1 - treated cells cDNA, 84.5 μL
- T2 - untreated cells cDNA, 84.5 μL
- T3 - no template control, 84.5 μL
5. Reaction set-up: loading the 96-well plate
The following illustrations use Plate Layout Variation 2...
|Pipette 19.5 μL of cDNA Template master mixes into the first well of each 3-well group
|Add 25.5 μL of Gene Target master mix to the cDNA. After each addition, mix by gently pipetting up and down 3 - 5 times (without making bubbles).
|Transfer 15 μL of solution from A1 into A2, and A3. Use a fresh pipette tip to do the same for A4-6, and A7-8.
|Repeat this procedure for the rest of the plate
- Seal the plate with clear film.
- The plate is now ready to load into the Light Cycler 480!


text:
VIDEO DOWNLOAD - Faceoff Vol. 11 - Missy Hyatt & Honky Tonk Man
Let me give you two names and you tell me what comes to your mind... Missy Hyatt and The Honky Tonk Man... controversial comes to my mind. When RF VIDEO sets out to conduct a new interview for our Face Off series we make sure we are going to give you a good bang for your buck..no pun intended there Missy.
Well that is exactly what happened when we sat down and had Missy and Honky paired up for the very first time for our newest Face Off volume 11 series. Both are no strangers to the RF VIDEO video cameras and everytime we turn our cameras on they both deliver with home runs. So we thought it would be a blast if we had them together for the very first time on one super DVD!!
What do you get when you put a couple that never holds back when you turn the camera on? A off the wall interview that will have you glued to your TV set for the entire length of the DVD. The interview starts off with Missy Hyatt asking HTM some questions that she wanted to ask for a long time. She did her homework as she had a ton of questions for him about his early days pre WWE. She asked him a lot about working in Memphis and the golden years during the territories. I quickly took over when she was done and asked them both about current events. You want to hear about what really happened with TNA and the HTM? Why is there heat with him and Bubba. What do they both think TNA needs to do to work. Both have some messages to Dixie Carter, Hogan and Eric Bischoff. Here are just some of the hot topics we touched upon:
You guys are both very open in public and online with your thoughts. Missy you've been very open with your sexual conquests and Honky you have talked a lot about rats. To relieve any sexual tension can you tell us if you two have ever hooked up before?
What wrestling marriages do you think were doomed for failure? Which ones are you surprised worked out?
What are your thoughts on Bubba the Love Sponge?
What are your thoughts on TNA letting go of Awesome Kong in favor of Bubba, only for Bubba to keep stalking her until he got fired?
What are your thoughts on TNA bombing on Monday nights?
What are your thoughts on Eric Bischoff?
If you were booking TNA, how would you change it, or is it just a dead end?
What are your thoughts about Virgil being back on WWF TV?
You guys do a lot of conventions. What people make you think "shit that person looks really bad"? and "Wow that person looks really good?" What are the best conventions you've done? The worst?
Honky, you put it out on your site you were going to TNA, only to say you were working everyone. Why did you do that?
Is it tough for both of you, being over 50 years old to make a living off the wrestling business? Do you guys have other source of income?
What are your thoughts on Eddie Graham?
Do you guys have any good indy stories, like stories that only would happen in indy wrestling?
Have you noticed that indy crowds are down? Why do you think that is?
What are your thoughts on Linda running for Senate?
Honky you are a friend of bret hart. What are your thoughts on his comeback to the WWe?
IS there anyone on current WWE TV that you think will be the next big star?
What are your thoughts on the Orlando Jordan gimmick in TNA?
Average Customer Review: Not yet rated
Write an online review and share your thoughts with others.


text:
Cops Gone Wild
Terrific editorial over at the Washington Times.
The bad behavior of these police officers exposes a double standard. As one Nationals fan, who is a lawyer, told us: "There's no way those cops could pass a street sobriety test right now. Just imagine how we'd get treated if they pulled us over having consumed half of what they've drunk tonight — and they're packing heat."
We don't begrudge police officers having a little fun, but they need to abide by the same laws they enforce on the rest of us. When they go out for a few beers, they might want to leave their uniforms and guns at home.
The idea of a National Peace Officers Memorial Week is a fine idea but it is regrettable that the memorial and event is in Washington, D.C. Just reinforces the wrongheaded notion that the federal government must be involved in everything.