目的:探讨S100A16蛋白对体重增加过程的影响,进一步研究S100A16蛋白在肥胖及肥胖相关疾病的发生发展过程中的作用?方法:正常8周龄大鼠随机分为正常饮食组(NF,n = 10)和高脂饮食组(HF,n = 10),建立饮食诱导的肥胖(DIO)大鼠模型,喂食14周时进行腹腔葡萄糖耐量试验(IPGTT)及胰岛素释放试验(IRT),喂食16周处死后称量皮下及内脏脂肪重量,采用HE染色方法 (hematoxylin and eosin staining,HE) 观察肝脏脂肪变性程度,放射免疫分析法检测血糖?血清胰岛素?尿酸等血清生化指标,同时应用Western blot方法检测脂肪组织中 S100A16及糖脂代谢相关转录因子的蛋白表达?结果:DIO组大鼠的体重?内脏脂肪明显高于正常组,血清总胆固醇和尿酸DIO组高于正常组但糖耐量和胰岛素释放低于正常组,Western blot显示DIO组大鼠脂肪和肝脏组织中S100A16?PPAR-γ?C/EBP-α的蛋白表达均高于正常组?结论:高脂饮食可上调S100A16及相关转录因子的表达;S100A16的高表达可促进脂质生成及肥胖发生,并对机体的胰岛素敏感性产生负性影响?
Objective:To investigate the effect of S100A16 on weight gain process and development of obesity and obesity related diseases. Methods:Rats aged 8 weeks were randomly divided into normal diet group(NF,n = 10) and high-fat diet group (HF,n = 10) to establish a diet induced obesity (DIO) rat model. After feeding for 14 weeks,intraperitoneal glucose tolerance test (IPGTT) and insulin release test (IRT) were performed in rats. After feeding for 16 weeks,rats were sacrificed and subcutaneous and visceral fat weights were measured. Then,HE staining method was used to observe the degree of liver steatosis,and radiation immunity analysis was used to detect serum biochemical indicators,such as blood sugar,insulin,serum uric acid. Western blot was performed to detect the expression of S100A16 and glucolipid metabolism related protein expression of transcription factors in adipose tissue. Results:The weight and visceral fat of rats in the DIO group were significantly higher than those in the normal group. The serum total cholesterol and uric acid in the DIO group was higher than normal glucose tolerance,and insulin release was lower than that of the normal group. Western blot showed that in liver and fat tissue of the DIO group,S100A16,PPAR-γ and C/EPB-α expression were significantly higher than those of the normal group. Conclusion:High fat diet can increase the expression of S100A16 and related expression of transcription factors;S100A16 over expression can promote lipid production and obesity,and result in a negative impact on insulin release and insulin sensitivity.