Glycogen content of the prawn muscles from the three groups showed increasing trend after 30 days of feed supplementation. In control animals recorded maximum of 2.2 mg/g, and in B. coagulans supplemented animals it was 4.1 mg/g and 3.6 mg/g in B. firmus supplemented animals increase in glycogen content in relation with feed supplementation with probiotic bacteria was most prominent in prawns. The increase in alpha amylases content may be attributed to the influence of the probiotic strain which possess exoenzyme activity. Maximum colonies were found in B. firmus supplemented animals gut, 962 cfu after 30 days of feed supplementation. Similar trend wasobserved in intestinal microflora of all three groups after infection in the feed supplemented animals. The one way analysis of variance showed significant variation among feed supplementation and treatment. The one way analysis revealed that B. coagulans supplemented group recorded maximum glycogen followed by B. firmus and control groups. Maximum THB count was observed in B. firmus supplemented animals after infecting the animals with V.parahaemolyticus. Alpha amylase activity was maximum in B. firmus supplemented animals was recorded. Significant results could be observed in one way analysis of variance.
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