Interleukin 28B (IL28B) which is also called as Interferon lambda3 (IFNλ3) has been analyzed for its immunomodulatory activity in mice. Bovine IL28B protein was administered to mice intramuscularly and then the spleenocytes were harvested from mice on 7th day post injection. The collected spleenocytes were analyzed for the activation of B and T lymphocytes using Fluorescent Activated Cell Sorting (FACS) analysis. Helper T cell activation using CD3-FITC, CD4-PE/Cy7 and CD69-PE; cytotoxic T cell activation using CD3-FITC, CD8-PE/Cy7 and CD69-PE and B cell activation using CD19-PE and CD22-FITC was studied. The results show that the IL28B protein activates both B and T lymphocytes. These immunomodulatory activities will have an added value in addition to its antiviral activity to elicit better immune response.
Keywords: Interleukin 28B (IL28B), mice, Spleenocytes, Lymphocytes, Fluorescent Activated Cell Sorting (FACS).