Source An E.coli strain, that carries the cloned HpySE 526I gene from Helicobacter pylori SE526.
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Storage conditions 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated with T4 DNA Ligase and recut.