Aaron Andalman – Design decisions when doing awake behaving electrophysiology.
Tom Davidson – Integrating optogenetics and electrophysiology in behaving animals.
Tom’s slides are here, and he has a handy-dandy laser alignment approach described here.
Simal Ozen Irmak – Multichannel recording systems.
The basic info for acquiring Ariel’s software, and the instructions to follow along with the tutorial can be found here!
for their assistance in making it happen!
Can anyone explain the reason why standard in vivo electrophysiology experiments use filters between 600-6000 Hz? This has been bugging me for a while now.
That bandpass is good for isolation of spikes, given their short timeframe. If one is interested in LFPs, you can drop your high pass, but typically those signals have bigger excursions than the spikes. Thus you’d either miss your spikes having lower gain amplification to catch the LFP, or you’d saturate the LFP trying to capture the spikes. For recording both spikes and LFP simultaneously, some people split the signal early, and then amplify a low bandpass at a lower gain, and a higher bandpass (e.g. 0.6 – 6kHz) to get spikes.
Thanks a lot for taking time to answer so thoroughly. I think I am starting to understand this a little better.