The Monarch DNA Gel Extraction Kit reliably purifies up to 5 µg of concentrated, high quality DNA from agarose gels. It utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design eliminates buffer retention and carryover of contaminants, enabling elution in volumes as low as 6 µl and with fewer steps. Monarch Buffers have been optimized, and do not require pH monitoring. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving a step. Eluted DNA is ready for use in restriction digestion, DNA sequencing, PCR, ligation, and other enzymatic manipulations.
What is the composition of each buffer provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
What size primers can be effectively removed from a PCR reaction?
Can the Monarch PCR & DNA Cleanup Kit (5 μg) be used to purify RNA?
Do you have any recommendations for purification of ssDNA?
Protocol Time: 10 min of spin and incubation time.