ASK1, GST-Fusion, Human, Recombinant, S. frugiperda: Malzeme Güvenlik Bilgi Formu (MSDS) veya SDS, Analiz Sertifikası (COA) ve Kalite Uygunluk Sertifikası (COQ), dosyalar, broşürler ve diğer dokümanlar.
Recombinant, human ASK1 (accession no. NM_005923), consisting of amino acids 649-946, fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6, is a serine/threonine kinase that is activated in response to TNFα. It plays a central role in stress-induced apoptosis; overexpression of ASK1 induces apoptotic cell death. The active kinase is useful in the study of ASK1 activity regulation and inhibitor screening.
The specific activity of ASK1 was measured as outlined above.
References Nishitoh, H. et al.1998. Molec. Cell 2, 389.
Ichijo, H. et al. 1997. Science 275, 90.
Unit of Definition Kinase activity is measured as the molar amount of phosphate incorporated into MBP at 30°C using a final concentration of 50 µM ATP.
Formulation In 150 mM NaCl, 50 mM Tris-HCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol, pH 7.5.
Nishitoh, H. et al.1998. Molec. Cell 2, 389.
Description Recombinant, human ASK1 (accession no. NM_005923), consisting of amino acids 649-946, fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6, is a serine/threonine kinase that is activated in response to TNFα. It plays a central role in stress-induced apoptosis; overexpression of ASK1 induces apoptotic cell death. The active kinase is useful in the study of ASK1 activity regulation and inhibitor screening.
Unit definition Kinase activity is measured as the molar amount of phosphate incorporated into MBP at 30°C using a final concentration of 50 µM ATP.