Qu, Hongyue; Luo, Wei; Lin, Qiang; Lin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
Abstract The population of lined seahorse (Hippocampus erectus) rapidly reduced because of the heavy exploitation in the wild. The single nucleotide polymorphism (SNP), known as one of the popular third-generation molecular markers can be developed easier and faster from high-throughput transcriptome sequencing. In this study, we developed 46 novel SNP markers based on transcriptome of H. erectus. Genetic diversity analysis showed that observed heterozygosity (Ho) and expected heterozygosity (He) were varied from 0.1724 to 0.5250 and from 0.1588 to 0.5000, respectively, and the minor allele frequency value was from 0.0625 to 0.5000. Seven loci were significantly different from Hardy-Weinberg equilibrium (P < 0.05) and significant linkage disequilibrium occurred between HeSNP24 and HeSNP38 (P < 0.01). The present study can provide an effective tool for population study and genetic breeding.
Corresponding Author Lin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
GB/T 7714 Qu, Hongyue,Luo, Wei,Lin, Qiang,et al. Development of SNP markers in lined seahorse (Hippocampus erectus) based on transcriptome sequencing[J]. CONSERVATION GENETICS RESOURCES,2016,8(1):2016-01-04.
APA Qu, Hongyue,Luo, Wei,Lin, Qiang,&Lin, Q .(2016).Development of SNP markers in lined seahorse (Hippocampus erectus) based on transcriptome sequencing.CONSERVATION GENETICS RESOURCES,8(1),2016-01-04.
MLA Qu, Hongyue,et al."Development of SNP markers in lined seahorse (Hippocampus erectus) based on transcriptome sequencing".CONSERVATION GENETICS RESOURCES 8.1(2016):2016-01-04.