Abstract : The aim of this study is to evaluate and mitigate the impact of a high-speed railway (HSR) line on functional connectivity for the European tree frog (Hyla arborea), an amphibian species highly sensitive to habitat fragmentation. The method consists of modeling its ecological network using graph theory before and after the implementation of the infrastructure and of evaluating changes in connectivity. This diachronic analysis helps visualize the potential impact of the HSR line and to identify areas likely to be most affected by the infrastructure.