Develop of new methods for the real-time monitoring of Pseuomona syringae pv. actinidiae directly in plant.
La ricerca mira a sviluppare metodi che consentano di monitorare in tempo reale e, in vivo, il processo infettivo della . syringae pv actnidia (PSA). A take scopo il batterio patogeno sarà trasformato inserendo nel material genetico il plasmide, pDSK-GFPuv, codificante per la una proteina autofuorescente GFPuv.
Infine, grazie a questi metodi, sarà possibile sviluppare le strategie agronomiche, culturale e fitoiatriche più efficaci nel controllare la malattia.
The research aims to develop a method allowing to monitor in vivo the plant-bacteria interactions during the infection process. These non-destructive methods will allow the direct visualization of the spatio-temporal interactions of P. syringae pv actnidia (PSA) in intact Actinidia chinensis tissues.
To reach this objective, a stable and broad host- range plasmid vector, pDSK-GFPuv, which expresses GFPuv (driven by a constitutive chloroplast promoter, psbA) at high levels will be inserted in competent PSA cells.
The GFPuv-labeled plant pathogenic bacteria not only can be easily visualized at the cellular level under a fluorescence microscope but also are clearly visible, as bacterial colonies, to the naked eye at the whole-plant level under longwavelength UV light. The plant-pathogen interaction will be successively studied be means of fluorescent steromicroscope and by confocal laser scanning microscopy.