Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human CAT1 (gene: SLC7A1).
This antibody gave a positive signal in the following human whole cell lysates: Jurkat, MCF-7 and HepG2.
Our Abpromise guarantee covers the use of ab37588 in the following tested applications.
WB 1/250. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues. May also function as an ecotropic retroviral leukemia receptor.
Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.
Additional bands at: 160 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab37588 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Additional bands at: 135 kDa, 155 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
IHC-FoFr image of CAT1 staining on Mouse Spinal Cord sections using ab37588 (1:1000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Publishing research using ab37588? Please let us know so that we can cite the reference in this datasheet.
1. Will both of these antibodies react with the mouse Slc7a1 receptor for purposes of immunocytochemistry or immunohistochemistry? If yes, do you have any images you can send for examples of its application?
2. What is the amino acid sequence and species of the immunogen?
3. Can you recommend a more effective antibody to label the mouse receptor, if you carry one?
4. Are you aware of any publications that have used these specific Abs? If yes, could you supply any relevant references?
5. Do you currently have thee eantibodies in stock?
6. Do you sell sample sizes?
7. Do you have a Canadian distributor?
The anti-SLC7A1 antibody ab37588 has been tested for reactivity with PFA-fixed, frozen mouse spinal chord by IHC. An image of the stain and a brief description of the protocol are available on the online datasheet. We are not aware of any other IHC or ICC testing. We know of one publication that used ab37588, and it is listed at the bottom of the online datasheet.
Click here (or use the following: https://www.abcam.com/index.html?datasheet=37588).
The antibody ab123956 has not, to our knowledge, been tested for reactivity with mouse samples or in any application other than western blotting. We are not aware of any publications for this antibody.
The human amino acid range that the immunogen is derived from, aa 428-457, LVLRYQPEQPNLVYQMASTSDELDPADQNE, has 83% identity with the mouse protein.
I am waiting for the originating laboratory to send alignment results for the exact immunogen sequence, contained within the range above.
Antibody ab37588 is in stock and available for delivery 2 days after placing your order directly with Abcam. Antibody ab123956 is not in stock and will be delivered 1-2 weeks after placing your order. Orders can also be placed through our Canadian distributor, Cedarlane, but we recommend placing orders directly through Abcam for fastest delivery.
We do not have samples sizes for the antibodies. We do have a testing discount program for untested applications and species, which are not covered by our guarantee.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information about the testing discount program.