英文摘要 Arsenic is common compounds in nature, and caused many public health problems in Taiwan. Organisms take in arsenic lead to abnormal physiological function. Low concentrations promote cell proliferation. High concentrations inhibit the metabolism and cause multiple organ disease. Generally, inorganic arsenic is usually more toxic than organic arsenic. Organisms take in inorganic arsenic the detoxification pathway is through the methylation into organic arsenic.
The zebrafish is an animal model in this thesis. Use solvent to extraction and freeze-dried the zebrafish product. Analyse metabolites include determination and quantification by NMR. Observe different arsenic conditions the metabolism research of zebrafish.
Experiment was found the zebrafish is enhanced methylation metabolism by arsenic. TMAO can as indicator metabolite of health status in zebrafish. Confirmed the combination of zebrafish and NMR provide a viable way of metabolic study.
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