Objectives: To assess oxidative damage and lipid profile levels in Type 2 diabetes mellitus patients with or without tobacco chewing habit. Methods: Total of 141 newly diagnosed Type 2 diabetes mellitus patients with tobacco chewing habit from past >18 years were included,136 Type 2diabetes mellitus without any tobacco chewing habit, 140 normal healthy subjects without any tobacco chewing habit were included in the study. Blood sample was collected after 8-12 hours fasting from each subject to estimate glucose and cholesterol. The plasma was used for estimation of total antioxidant (TAA) activity, Vitamin C and thiobarbituric acid reacting substances (TBARS) by standard methods. Statistical analysis was done with SPSS version 16.Results: The glucose, cholesterol and TBARS levels were found to be increased significantly (p<0.001) in diabetic patients with tobacco chewing habit, as well as TAA, Vitamin C levels decreased significantly (p<0.001) when compared with healthy individuals. Conclusion: We observed increased oxidative stress and declined levels of antioxidants in newly diagnosed type 2 diabetes patients with tobacco chewing habit. It is known that hyperglycemia induces oxidative stress and further facilitate the progression of diabetic complications.