The Mitochondrial Laboratory focuses on evaluation of respiratory activity of isolated mitochondria or mitochondria in situ, i.e. in tissues or cells after mechanical and/or chemical permeabilization. The method of high-resolution respirometry extended by fluorimetric analysis of selected mitochondrial parameters enables simultaneous measurement of oxygen flux, reactive oxygen species production, mitochondrial membrane potential and ATP production in tissue biopsies, homogenates, intact or permeabilized cells (cultured cell lines, platelets, spermatozoa) and isolated mitochondria.