In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3′- ends of the internucleosomal DNA strand breaks that are a hallmark of apoptosis. The CF® dye TUNEL Assay Apoptosis Detection Kit employs dUTP conjugated to the exceptionally bright and photostable fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry.
The kit is available with the following CF® dyes: CF®488A, CF®594, and CF®640R.
2. Gold et al. (1994). Lab Invest. 71(2):219-25.
3. PLoS ONE 8(1), e53657 (2013).