A kind of GNPs/GC modified electrode was prepared by electrodeposited gold nanoparticles on the surface of glassy carbon electrode based on the cyclic voltammetry. Electrochemical properties and surface morphology of the modified electrode were characterized. The electrochemical behaviors of acetaminophen (ACOP) at GNPs/GC electrode were investigated by cyclic voltammetry and electrochemical impedance spectrum (EIS). The influences of different experimental conditions on the electrode performance were discussed. Under optimal conditions, a linear relationship of ACOP concentration with peak current was obtained over the concentration range of 2.0 × 10−7 - 2.0 × 10−5 mol∙L−1 and 1.4 × 10−4 - 3.78 × 10−3 mol∙L−1, respectively. The detection limit was 3.6 × 10−8 mol∙L−1 (S/N = 3:1) and the recovery was 97.7% - 101.0%.
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