Zyagen offers high quality pure intact total RNA of of almost all major tissues of CD1 (ICR) mouse strain that might difficult to obtain because of size, anatomical complexity, or high RNase tissue content.
Total RNA is routinely extracted from single healthy animal using the powerful classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction..
Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice. Sample can also be provided in 1mM sodium citrate, 0.1 mM EDTA, or any other RNA storage buffer.
Custom Services: Zyagen also offers custom services for isolation of total RNA and mRNA of any tissue of any mouse strain or any other species. Contact technical services for more information.