Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ERK2/MAPK1 Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C.
L1 rat brain, L2 rat heart lysates probed (bs-0022R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 42kDa.
Mouse placenta lysates probed with ERK1 + ERK2 Polyclonal Antibody, unconjugated (bs-0022R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.