The specificity of T-cell receptor to an antigen is determined by the five variable elements of the a and ß chains. Research scientists at National Jewish Health have developed a method to produce monoclonal antibodies against any of the five variable elements of human T-cell receptor. This method relies on the transfection of human/mouse hybrid T-cell receptor a or ß chains genes into a mouse T-cell hybridoma lacking an endogenous a or b chain gene.
Monoclonal antibodies for therapeutic use.
Method adaptable to any of the five variable elements of the human T-cell receptor.
hVß elements can be expressed on the surface of mouse T-cell hybridomas.
The transfected T-cell hybrids are excellent immunogens in mice for hVß.
Monoclonal antibodies against hVß13.1 and -13.2 can be raised and be used for the measurements of Vß13.1 and -13.2 in humans.
Available for licensing on a non-exclusive basis.