Author(s): Suratna Hazra, Ph.D., Grigoriy Tchaga, Ph.D., and Gordon Yan, Ph.D.
Abstract: Carbonic Anhydrase (CA) is a zinc based metalloprotease present in at least 14 different isoforms in mammals. It catalyzes the reversible hydration of carbon dioxide to form bicarbonate and proton, thus playing an important role in pH and CO2 homeostasis. Changes in CA activity are associated with various diseases such as - glaucoma, type II diabetes mellitus, lung, liver diseases etc. As CA is also an important target for cancer therapy, a robust and sensitive high-throughput carbonic anhydrase activity and inhibitor screening assays are very important tools.