TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions.
RSAFD1 MaxPab polyclonal antibody. Western Blot analysis of RSAFD1 expression in human colon.
Western Blot analysis of TYW1 expression in transfected 293T cell line (H00055253-T01) by TYW1 MaxPab polyclonal antibody.Lane 1: RSAFD1 transfected lysate(42.24 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of TYW1 in human lung tissue lysate with TYW1 antibody at 1 ug/mL.
Immunohistochemistry of TYW1 in human lung tissue with TYW1 antibody at 5 ug/mL.