INTENDED USE This Chicken APOP ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken APOP in the sample, this Chicken APOP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Chicken APOP concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.