SpecificityThis assay has high sensitivity and excellent specificity for detection of human CD2AP. No significant cross-reactivity or interference between human CD2AP and analogues was observed.
Intended UseHuman CD2AP ELISA Kit allows for the in vitro quantitative determination of CD2AP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD2AP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD2AP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD2AP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD2AP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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