it is noted in the figure legend that Dylight 488-conjugated goat anti-mouse IgG were used. I am not sure it is correct secondary antibodies since the primary antibodies are rabbit.
How were cells' permeabilized for flow?
I'm sorry for the confusion here. The secondary should be referred to as an 'anti-rabbit IgG'.
Cells were permeabilized for flow by incubation with ice cold 90% methanol for 30 min on ice.
It seems this protein is intracellular but the flow data does not indicate use of a reagent to permeabilize cells. So I just want to clarify the sub cellular localization and the protocol. thanks.
What should be noted in the figure legends is that these cells were permeabilized with 90% ice cold methanol for 30 minutes.
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