Reacts with Avian Influenza A. Not yet tested in other species.
Synthetic peptide corresponding to 16 amino acids in the middle of the Neuraminidase protein. Efforts were made to use relatively conserved regions as the antigen.
Our Abpromise guarantee covers the use of ab21305 in the following tested applications.
ELISA: Use at a concentration of 1 µg/ml, detects 10 ng of free peptide.
Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.
Cell Membrane; Virion membrane. Apical cell membrane; Single-pass type II membrane protein (By similarity).
Publishing research using ab21305? Please let us know so that we can cite the reference in this datasheet.
Thank you for kindly providing the sequence. This has been checked, and has the following percentage alignment with the following two antibodies: ab21304 Avian Influenza A Neuraminidase antibody: 80% ab21305 Avian Influenza A Neuraminidase antibody: 87% We recommend alignment shoudl be over 85% to predict that an antibody will detect. Therefore, there is likely to be some crossreactivity with ab21304, but ab21305 may be a better choice based on the alignment scores. Please check the online datasheet to ensure this has been tested in the application the customer would like to use. Click here (or use the following: https://www.abcam.com/index.html?datasheet=21305). I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.
Dear Sir/Madam My name is Shenshen Cai, currently working at University of Pennsylvania on some antigenic peptide projects. I see some H5N1 antibodies in your catalog. May I know the sequences of the immunogenic peptides you used to raise your antibodies ab21292, ab21305, ab21297, ab21304? thank you very much, I look forward to hearing from you.
Thank you for your enquiry. Unfortunately, the immunogen sequences for ab21292, ab21297, ab21304 and ab21305 are proprietary. However, I can give you a range from which we selected the peptides. ab21305 - residues 150-200 ab21304 – residues 400-450 ab21292 – residues 300-350 ab21297 – residues 100-150 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.
Thank you for your enquiry and your interest in our products. This product should detect the protein. Unfortunately, we have only been able to test the antibody against the immunogen peptide in ELISA.