# Validation of conductance catheter system for quantification of murine pressure-volume loops

Bo Yang, Douglas F Larson, Julie Beischel, Ryan Kelley, Jiaqi Shi, Ronald R Watson

Research output: Contribution to journalArticle

42 Citations (Scopus)

### Abstract

The purpose of this study was to define the validation methods and outcomes of a conductance catheter system specifically for in vivo murine cardiac hemodynamic analysis. To express the relationship between conductance and blood volumes, we used an in vitro model to derive a volume-conductance line. The volume-conductance line was used to compute raw volume from the modified conductance signals. The parallel volume was calibrated with hypertonic (15%) saline injected from extrajugular vein. The ventricular volume was computed by raw volume minus parallel volume. The accuracy of conductance volumetric measurements was validated with a static in situ infusion of calibrated volumes of whole blood injected into arrested left ventricles. In vivo dynamic measurements were performed with 24 C57B1/6 mice, 6 months old; for comparison of established values. The in situ model showed that after calibration, the experimental coefficient, α, was equal to 1 and the measured volume by conductance catheter was equal to the true volume of the left ventricle (y = 0.982× + 0.513, p < .0001). For the in vivo models, the end-diastolic volumes and the stroke volumes and cardiac output determined with the conductance catheter system were 17.3 ± 1.0 μL, 10.6 ± 0.9 μL, and 6.0 ± 0.5 mL/min, respectively. We validated the relationship between measured volume by conductance catheter and the true volume and demonstrated the accuracy of the volume-conductance line for conversion of conductance to volume.

Original language English (US) 341-355 15 Journal of Investigative Surgery 14 6 https://doi.org/10.1080/089419301753435710 Published - 2001

Catheters
Pressure
Blood Volume
Heart Ventricles
Cardiac Output
Stroke Volume
Calibration
Veins
Hemodynamics

### Keywords

• Conductance
• ESPVR
• Hemodynamics
• Mouse
• PRSW
• Ventricular function
• Ventricular mechanics

• Surgery

### Cite this

Validation of conductance catheter system for quantification of murine pressure-volume loops. / Yang, Bo; Larson, Douglas F; Beischel, Julie; Kelley, Ryan; Shi, Jiaqi; Watson, Ronald R.

In: Journal of Investigative Surgery, Vol. 14, No. 6, 2001, p. 341-355.

Research output: Contribution to journalArticle

title = "Validation of conductance catheter system for quantification of murine pressure-volume loops",
abstract = "The purpose of this study was to define the validation methods and outcomes of a conductance catheter system specifically for in vivo murine cardiac hemodynamic analysis. To express the relationship between conductance and blood volumes, we used an in vitro model to derive a volume-conductance line. The volume-conductance line was used to compute raw volume from the modified conductance signals. The parallel volume was calibrated with hypertonic (15{\%}) saline injected from extrajugular vein. The ventricular volume was computed by raw volume minus parallel volume. The accuracy of conductance volumetric measurements was validated with a static in situ infusion of calibrated volumes of whole blood injected into arrested left ventricles. In vivo dynamic measurements were performed with 24 C57B1/6 mice, 6 months old; for comparison of established values. The in situ model showed that after calibration, the experimental coefficient, α, was equal to 1 and the measured volume by conductance catheter was equal to the true volume of the left ventricle (y = 0.982× + 0.513, p < .0001). For the in vivo models, the end-diastolic volumes and the stroke volumes and cardiac output determined with the conductance catheter system were 17.3 ± 1.0 μL, 10.6 ± 0.9 μL, and 6.0 ± 0.5 mL/min, respectively. We validated the relationship between measured volume by conductance catheter and the true volume and demonstrated the accuracy of the volume-conductance line for conversion of conductance to volume.",
keywords = "Conductance, ESPVR, Hemodynamics, Mouse, PRSW, Ventricular function, Ventricular mechanics",
author = "Bo Yang and Larson, {Douglas F} and Julie Beischel and Ryan Kelley and Jiaqi Shi and Watson, {Ronald R}",
year = "2001",
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TY - JOUR

T1 - Validation of conductance catheter system for quantification of murine pressure-volume loops

AU - Yang, Bo

AU - Larson, Douglas F

AU - Beischel, Julie

AU - Kelley, Ryan

AU - Shi, Jiaqi

AU - Watson, Ronald R

PY - 2001

Y1 - 2001

N2 - The purpose of this study was to define the validation methods and outcomes of a conductance catheter system specifically for in vivo murine cardiac hemodynamic analysis. To express the relationship between conductance and blood volumes, we used an in vitro model to derive a volume-conductance line. The volume-conductance line was used to compute raw volume from the modified conductance signals. The parallel volume was calibrated with hypertonic (15%) saline injected from extrajugular vein. The ventricular volume was computed by raw volume minus parallel volume. The accuracy of conductance volumetric measurements was validated with a static in situ infusion of calibrated volumes of whole blood injected into arrested left ventricles. In vivo dynamic measurements were performed with 24 C57B1/6 mice, 6 months old; for comparison of established values. The in situ model showed that after calibration, the experimental coefficient, α, was equal to 1 and the measured volume by conductance catheter was equal to the true volume of the left ventricle (y = 0.982× + 0.513, p < .0001). For the in vivo models, the end-diastolic volumes and the stroke volumes and cardiac output determined with the conductance catheter system were 17.3 ± 1.0 μL, 10.6 ± 0.9 μL, and 6.0 ± 0.5 mL/min, respectively. We validated the relationship between measured volume by conductance catheter and the true volume and demonstrated the accuracy of the volume-conductance line for conversion of conductance to volume.

AB - The purpose of this study was to define the validation methods and outcomes of a conductance catheter system specifically for in vivo murine cardiac hemodynamic analysis. To express the relationship between conductance and blood volumes, we used an in vitro model to derive a volume-conductance line. The volume-conductance line was used to compute raw volume from the modified conductance signals. The parallel volume was calibrated with hypertonic (15%) saline injected from extrajugular vein. The ventricular volume was computed by raw volume minus parallel volume. The accuracy of conductance volumetric measurements was validated with a static in situ infusion of calibrated volumes of whole blood injected into arrested left ventricles. In vivo dynamic measurements were performed with 24 C57B1/6 mice, 6 months old; for comparison of established values. The in situ model showed that after calibration, the experimental coefficient, α, was equal to 1 and the measured volume by conductance catheter was equal to the true volume of the left ventricle (y = 0.982× + 0.513, p < .0001). For the in vivo models, the end-diastolic volumes and the stroke volumes and cardiac output determined with the conductance catheter system were 17.3 ± 1.0 μL, 10.6 ± 0.9 μL, and 6.0 ± 0.5 mL/min, respectively. We validated the relationship between measured volume by conductance catheter and the true volume and demonstrated the accuracy of the volume-conductance line for conversion of conductance to volume.

KW - Conductance

KW - ESPVR

KW - Hemodynamics

KW - Mouse

KW - PRSW

KW - Ventricular function

KW - Ventricular mechanics

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DO - 10.1080/089419301753435710

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VL - 14

SP - 341

EP - 355

JO - Journal of Investigative Surgery

JF - Journal of Investigative Surgery

SN - 0894-1939

IS - 6

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