Looking over them, Leicester is astonished to discover among their number Matilde, the wife he has wedded secretly, now in male attire together with her brother Enrico. When the Queen and her courtiers retire, Leicester remains alone with Matilde and scolds her for her rashness; in fact she is the daughter of Mary, Queen of Scots, and therefore banished from England like all the descendants of the beheaded Scottish Queen. Matilde explains that she has rushed to her husband’s side because of insistent rumours that Elizabeth is in love with him; Enrico, joining them, adds that he was unable to restrain her, so he came with her to protect her. Leicester decides that he must not be seen in the company of his wife and brother-in-law, to avoid arousing suspicion; it will be better to ask the advice of Norfolc, whom he believes to be a true friend. Matilde, who is rather upset, trusts that the future will bring peace of mind.
Leicester decide che è meglio non mostrarsi accanto alla consorte e al cognato, per non ingenerare sospetti; chiederà consiglio piuttosto a colui che ritiene un vero amico, Norfolc. Matilde, sconfortata, si augura di trovare presto un momento di serenità.