Larger fungi (usually referred to as macrofungi or macromycetes) are of particular interest because of their importance as food resources and as a component of traditional culture in many places in the world. Moreover, many basidiomycetes and ascomycetes with conspicuous sporocarps often play an important role as ectomycorrhizal mycobionts of trees and shrubs of boreal forests in the northern hemisphere and are important elements in many areas of the southern hemisphere. Although Guatemalan macrofungi have not been as yet extensively surveyed, a preliminary checklist encompasses some 350 species of macromycetes (31 ascomycetes and 319 basidiomycetes) occurring in 163 genera and 20 ascomycetous and basidiomycetous orders. Recently, 12 species of Ascomycetes where cited, with the new records, there are now 44 ascomycete species known from Guatemala Most available observations pertain to the highlands, in the departments of Alta Verapaz, Baja Verapaz, Chimaltenango, Guatemala, El Quiché, Huehuetenango, and Quetzaltenango, while the wide lowland Petén region has been scantly explored, despite the fact that it accounts for about one third of Guatemala's area and, together with adjacent areas of Belize and southern Mexico, comprise the largest unbroken tract of tropical forest north of the Brazilian Amazon. At the order level, Agaricales was found to host the larger number of species (almost one third of the entire set), followed by Polyporales and Boletales. The most represented genera are Amanita, Russula, Lactarius, Laccaria, Suillus. Intriguingly, all these genera are ectomycorrhizal with the several Pinus and Quercus species that form extensive pine and mixed forests of the highlands, and/or with the endangered Abies guatemalensis (pinabete), most abundant between 2800-3200 m elevation on the Sierra de los Cuchumatanes in western Guatemala.