Wolf Run is a tributary of North Branch Mehoopany Creek in Bradford County and Sullivan County, in Pennsylvania, in the United States. It is approximately long and flows through Wilmot Township in Bradford County and Colley Township in Sullivan County. The stream's watershed has an area of , most of which is agricultural land and woodlots. In a 2001 study, seven species of fish were found in Wolf Run, but no gamefish. More recently, wild trout have been observed as well.