PA 326 begins at the Maryland border in Southampton Township, where the road connects to Black Valley Road and Gap Road NE south of the state line. From this point, the route heads north on two-lane undivided South Black Valley Road, passing through an agricultural valley with some woods and homes. The road curves more to the northeast, turning east onto Chaneysville Road and passing through a gap in Warrior Ridge. PA 326 reaches the community of Hewitt, at which point the road winds north through farmland and woodland in another narrow valley. The route continues through the valley for several miles, curving east before turning north onto Chaneysville Cove Road near the community of Chaneysville. The road curves northwest and passes through another gap in warrior ridge, at which point it passes through a portion of the Buchanan State Forest. PA 326 turns north onto Black Valley Road and runs through forested areas with some farms. The route bears west onto Main Road, turning west into forests and crossing Tussey Mountain. After a bend to the north, the road heads into Monroe Township and passes through more of the Buchanan State Forest.