Pristimantis jamescameroni is so far known only from the summit of Aprada-tepui at Bolívar state, Venezuela, where it is found at an elevation range between above sea level. The habitat is mainly covered by open rock vegetation and small islands of tepui forests. The region is particularly characterized by numerous small lakes and some deep canyons. The main habitat area is apparently the underneath of shallow rocks. The holotype was collected during day on the ground while calling in a small shallow rock crevice, hidden by the vegetation. All other specimens were collected under rocks. Other males could be heard calling very sporadically from shallow rock crevices by day. Due to their limited distribution, Kok suggested the frogs be classified as Endangered by the IUCN Red List of Threatened Species.