Asthall barrow is located along the strip of the A40 connecting the towns of Witney and Burford; it is immediately north of the A40 where it connects to Burford road, and just south of the byroad leading off Burford to Asthall and Swinbrook. The barrow is prominently located, and gives its name to surrounding structures, such as Barrow Plaintation, Barrow Farmhouse, and Asthall Barrow Roundabout. It overlooks the Thames Valley from Lechlade to Wytham Hill. To the north it looks out at Leafield barrow; to the south appears Faringdon Hill and beyond it the Berkshire Downs at White Horse Hill, while to the southeast Sinodun Hill near Dorchester appears, with the Chilterns in the distance.