In his book Studies on zoogeographical divisions for fresh water fishes of China, Li divided the fauna of freshwater fishes in China into five major regions based on characteristics of fish species distributions, geographic environments and geological histories of these regions. According to his method, the demarcation line between Holarctic and Indomalayan realms in China, as far as freshwater fishes are concerned, lies largely along the Himalayas and Nanling Mountains ranges. This contrasts with the prevalent view of demarcation line between Holarctic and Indomalaya regions along the Qinling Mountains, even though the latter does not extend into Eastern part of China. Freshwater Ecoregions of the World (FEOW), a collaborative global biodiversity project partly sponsored by World Wide Fund for Nature (WWF), cites this book as a source of references in delineation of freshwater ecoregions of China.