Engineering silica encapsulated composite of acyltransferase from Mycobacterium smegmatis and MIL-88A A stability-and activity-improved biocatalyst for N-acylation reactions in water.
Here the metal-organic framework material MIL-88A is used to purify and immobilize an acyltransferase from Mycobacterium smegmatis (MsAcT) simultaneously from the broken bacterial liquid. Regarding the possibility that the MsAcTMIL-88A may display weak stability in its application, a silica layer is further introduced around it as a shield to protect the enzyme from degradation. The obtained MsAcTMIL-88Asilica can exhibit high activity recovery, excellent thermal, pH, and storage stabilities compared with those of MsAcTMIL-88A. The MsAcTMIL-88Asilica can also be effectively recycled, and its initial activity of 84.0 % ± 1.2 % can be retained after the 5th cycle for N-acylation reaction in water. More importantly, the MsAcTMIL-88Asilica can display much higher catalytic activity towards the reactions between ethyl or vinyl esters and aniline than those of free MsAcT and MsAcTMIL-88A in aqueous media. This study provides a simple and inexpensive strategy to prepare MsAcTMIL-88Asilica with high activity, stability, and excellent recyclability, and highlights its application potential as a biocatalyst.