Short Communication Evaluation of antimicrobial activities of Harmine, Harmaline, Nicotine and their complexes.
Harmine, Harmaline, Nicotine and its various complexes synthesized have been characterized by physical, spectral and analytical methods and curtained for in-vitro antimicrobial activity against different bacterial and fungal species at two different concentrations i.e.100μ100µl and 200μ100µl dose level respectively. Analysis showed that Nicotine, Zinc-Nico, Cd-Nico, Hg-Nico, Ni-Nico, Cu-Nico, Co-Nico, Harmine, and Harmaline having conc. of 100ug 100ul had antibacterial activity on zero, 5, 4, 10, zero, 5, 7, zero, zero strain of bacteria having an average of zero (SD0.0000), 15.2000 (SD1.30384), 18.2500 (SD3.30404), 20.2000 (SD1.39841), zero (SD0.0000), 14.6000 (SD0.89443), 15.8571 (SD1.34519), zero (SD0.0000), zero (SD0.0000) respectively. Zinc (II) chloride, Cadmium (II) Iodide, Mercury (II) chloride, Nickel (II) chloride, Copper (II) chloride, Cobalt (II) chloride, Mercury (II) chloride, Mercury (II) harmine, Mercury (II) harmaline at 100ug100ul is valid for 7, 8, 9, 2, 7, 8, 9, 10, 8 strains of bacteria with an average of 7.1429 (SD1.06904), 10.0000 (SD5.01427), 14.8889 (SD6.00925), 6.0000 (SD0.0000), 8.5714 (SD4.27618), 8.2500 (SD0.88641), 14.8889 (SD6.00925), 18.6000 (SD2.45855), 18.5000 (SD1.85164) respectively. The above given compounds at the conc. of 200 μ100ul is valid for 10, 9, 10, 8, 8, 10, 10, 10, 10 strains of bacteria with an average of 8.1 (SD1.66333), 11.7778 (SD5.28625), 16.1000 (SD6.36745), 6.5000 (SD0.92582), 9.7500 (SD4.43203), 9.9000 (SD2.76687), 16.1000 (SD6.36745), 22.0000 (SD2.44949), 20.4000 (SD2.75681) respectively. The above given compounds at conc. of 200 μ100ul showed antibacterial action on 3, 8, 8, 10, 3, 9, 8, zero, 3 strains of bacteria with an average of 14(SD0.000), 16.8750 (SD1.35620), 18.2500 (SD3.45378), 22.7000 (SD1.82878), 14.3333 (SD0.57735), 16.7778 (SD1.71594), zero (SD0.000), 12.0000 (SD1.00000) respectively. Hence according to the average value of the zone of inhibition, maximum antibacterial activity at 100-200ug100ul is of Hg-Nico and Mercury salt Mercury (II) harmine having an average of 20.2000 (SD1.39841)-22.7000 (SD1.82878) and 18.6000 (SD2.45855)-22.0000 (SD2.44949). Minimum antibacterial activity at 100-200ug100ul is Nicotine100, Nicotine-Nico100, Harmine 100,Harmaline 100, Harmine 200 having zero average (SD0.000).