LC-MSMS Assay for the Measurement of Cannabidiol Profiling in CBD Oil from Japanese Market and Application for Convertible Tetrahydrocannabinol in Acetic Acid Condition.
Cannabidiol (CBD), a major non-psychoactive cannabinoid, has a lot of attention due to its potential relaxing properties and led the trend in commercial CBD aromaoral hemp seed oil from the Japanese market. In this study, a routine assay for evaluating CBD oil samples was performed using LC coupled with tandem mass spectrometry (LC-MSMS) and was used to apply the convertible tetrahydrocannabinol (THC) in acetic acid conditions. Based on the electrospray positive ion mode, the detection of cannabidiolic acid (CBDA mz 359 > 219), cannabigerolic acid (CBGA mz 361 > 343), cannabigerol (CBG mz 317 > 193), CBD (mz 315 > 193), THC (mz 315 > 193) and cannabinol (CBN mz 311 > 223) was performed by satisfying separation with high density of C