N,N-dimethyltryptamine Prevents Renal Ischemia-Reperfusion Injury in a Rat Model.
Ischemia reperfusion (IR) injury remains one of the most challenging fields of organ transplantation. It is highly associated with the use of expanded criteria donors that might conclude to delayed graft function or early or late graft failure. To investigate the metabolic, microcirculatory parameters, and histologic changes under the effect of N,N-dimethyltryptamine (DMT) in a renal IR model in rats. In 26 anesthetized rats both kidneys were exposed. In the control group (n 6) no other intervention happened. In 20 other animals, the right renal vessels were ligated, and after 60 minutes the right kidney was removed. The left renal vessels were clamped for 60 minutes then released, followed by 120 minutes of reperfusion. In the IR group (n 10), there was no additive treatment, while in IR DMT group (n 10) DMT was administered 15 minutes before ischemia. Blood samples were taken, laser Doppler measurement was performed, and both kidneys were evaluated histologically. Microcirculation (blood flux units BFU) diminished in all groups, but remarkably so in the IR DMT group. This group compensated better after the 30th minute of reperfusion. The control and IR DMT groups had similar BFUs after 120 minutes of reperfusion, but in the IR group BFU was higher. Tubular necrosis developed in the IR and IR DMT groups too it was moderated under DMT effect, and severe without. Histologic injuries were less in IR DMT Group compared to non-treated animals. Histologic changes characteristic to IR injuries were reversible and microcirculation recovered at the end of 120 minutes reperfusion under the administration of DMT. DMT can be used for renoprotection in kidney transplantation.