Cold Ethanol Extraction of Cannabinoids and Terpenes from Cannabis Using Response Surface Methodology Optimization and Comparative Study.
Efficient cannabis biomass extraction can increase yield while reducing costs and minimizing waste. Cold ethanol extraction was evaluated to maximize yield and concentrations of cannabinoids and terpenes at different temperatures. Central composite rotatable design was used to optimize two independent factors sample-to-solvent ratio (12.9 to 117.1) and extraction time (5.7 min-34.1 min). With response surface methodology, predicted optimal conditions at different extraction temperatures were a cannabis-to-ethanol ratio of 115 and a 10 min extraction time. With these conditions, yields (g 100 g dry matter