Subchronic PCP effects on DNA methylation and protein expression of NMDA receptor subunit genes in the prefrontal cortex and hippocampus of female rats.
N-methyl-d-aspartate receptor (NMDAR) dysfunction is implicated in schizophrenia, and NMDAR antagonists, such as phencyclidine (PCP), can induce behaviours that mimic aspects of the disorder. We investigated DNA methylation of Rats were divided into two groups that received vehicle (0.9% saline) or 2 mgkg PCP twice a day for 7 days (n 10 per group). After behavioural testing (novel object recognition), to confirm a cognitive deficit, brains were dissected and NMDAR subunit DNA methylation and protein expression were analysed by pyrosequencing and ELISA. The scPCP administration led to The scPCP treatment resulted in increased DNA methylation at promoter sites of