Analysis of tetrahydrocannabinol derivative from cannabis-infused chocolate by QuEChERS-thin layer chromatography-desorption electrospray ionization mass spectrometry.
Recently in Canada and some states of the United States, marijuana (cannabis) has become fully legalized and regulated, for both medical and recreational purposes. This fact is going to make cannabis products such as edibles even more popular than ever before. Therefore, it is assumed that there will be a high demand for analytical methods, which are accurate and sensitive enough to be used in different forensic and pharmaceutical cannabis-related applications. Cannabis derivatives have an extreme range and number of constituents with possible interactions with one another. Thus, this characteristic leads to their vast and highly complex chemistry, which requires robust analytical tools to be able to precisely and accurately quantify and qualify them. We developed and validated an analytical method using desorption electrospray ionization (DESI)-mass spectrometry (MS) to accurately detect, characterize, and quantify cannabinoids and also offer an easy, cost-effective, and reliable technique, which can be performed in a short time for infused edibles in complex matrices such as chocolate. We evaluated a quantitative analysis of tetrahydrocannabinol (THC) in cannabis-infused chocolate with thin-layer chromatography (TLC)-DESI-MS and QuEChERS extraction method. Both techniques of TLC and QuEChERS are cost-effective and can be run in short time.