Estrogen exerts anti-inflammatory effects by inhibiting NF-κB pathway through binding with estrogen receptor β on synovicytes of osteoarthritis.
Objective To investigate the mechanism of estrogens anti-inflammatory effects on synovial cells during the pathogenic process of osteoarthritis. Methods We isolated synovicytes from synovium tissues and identified the cells with flow cytometry. Then we detected the expression level of estrogen receptor β (ERβ) in synovicytes with immunofluorescence staining. The synovicytes were divided into control group, group pretreated with 10 ngmL IL-1β, group pretreated with 10 ngmL IL-1β and 10