Development of a validated method for rapid quantification of up to sixteen cannabinoids using ultra-high-performance liquid chromatography diode-array detector with optional electrospray ionization time-of-flight mass spectrometry detection.
Due to the recent legalization of medical and recreational Cannabis in many countries in the world, there has been an increasing demand for accurate quantification of a growing number of cannabinoids. To meet this challenge, a method for rapid quantification of up to sixteen cannabinoids using ultra-high-performance liquid chromatography diode-array detector (UHPLC-DAD) has been developed, validated and used in the analysis of hemp concentrates. While published LC-UV methods were usually for twelve or less cannabinoids and might not achieve baseline separation of some critical pairs of cannabinoids, e.g., CBGCBD (cannabigerolcannabidiol) and Δ