role of mercuric chloride in plant tissue culture
surface sterilisation of explants
title: Meristem text: Meristem A meristem is the tissue in most plants containing undifferentiated cells (meristematic cells), found in zones of the plant where growth can take place. Meristematic cells give rise to various organs of a plant and are responsible for growth. Differentiated plant cells generally cannot divide or produce cells of a different type. Meristematic cells are incompletely or not at all differentiated, and are capable of continued cellular division. Therefore, cell division in the meristem is required to provide new cells for expansion and differentiation of tissues and initiation of new organs, providing the basic structure of the plant body. title: Plant tissue culture text: from preexisting meristems (shoot culture or nodal culture), organogenesis and non-zygotic embryogenesis. The propagation of shoots or nodal segments is usually performed in four stages for mass production of plantlets through in vitro vegetative multiplication but organogenesis is a common method of micropropagation that involves tissue regeneration of adventitious organs or axillary buds directly or indirectly from the explants. Non-zygotic embryogenesis is a noteworthy developmental pathway that is highly comparable to that of zygotic embryos and it is an important pathway for producing somaclonal variants, developing artificial seeds, and synthesizing metabolites. Due to the single cell origin of non-zygotic embryos, title: Plant tissue culture text: are the meristematic ends of the plants like the stem tip, axillary bud tip and root tip. These tissues have high rates of cell division and either concentrate or produce required growth regulating substances including auxins and cytokinins. Shoot regeneration efficiency in tissue culture is usually a quantitative trait that often varies between plant species and within a plant species among subspecies, varieties, cultivars, or ecotypes. Therefore, tissue culture regeneration can become complicated especially when many regeneration procedures have to be developed for different genotypes within the same species. The three common pathways of plant tissue culture regeneration are propagation title: Meristem text: a reservoir of stem cells to replenish whatever is lost or damaged. Root apical meristem and tissue patterns become established in the embryo in the case of the primary root, and in the new lateral root primordium in the case of secondary roots. In angiosperms, intercalary meristems occur only in monocot (in particular, grass) stems at the base of nodes and leaf blades. Horsetails also exhibit intercalary growth. Intercalary meristems are capable of cell division, and they allow for rapid growth and regrowth of many monocots. Intercalary meristems at the nodes of bamboo allow for rapid stem elongation, while those title: Tissue culture text: Tissue culture Tissue culture is the growth of tissues or cells separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle