Selain unsur-unsur kimia di atas, jintan hitam juga mengandung minyak esensial, 15 asam amino (alanin, arginin, isoleusin, lisin, triptofan, tirosin, treonin, asaparagin, sistin, glisin, asam glutamat, metionin, dan prolin), zat besi, natrium, kalium, tiamin, riboflavin, piridoxin, niasin, copper, dan zinc.