rna splicing is the type of enzyme used to produce the cdna.
name the type of enzyme used to produce the cdna?
rna splicing
the protocols for 5' or 3' races differ slightly. 5' race-pcr begins using mrna as a template for a first round of cdna synthesis (or reverse transcription) reaction using an "anti-sense" (reverse) oligonucleotide primer that recognizes a known sequence in the middle of the gene of interest; the primer is called a "gene specific primer" (gsp). the primer binds to the mrna, and the enzyme reverse transcriptase adds base pairs to the 3' end of the primer to generate a specific single-stranded cdna product; this is the reverse complement of the mrna. following cdna synthesis, the enzyme terminal deoxynucleotidyl transferase (tdt) is used to add a string of identical nucleotides, known as a homopolymeric tail, to the 3' end of the cdna. (there are some other ways to add the 3'-terminal sequence for the first strand of the de novo cdna synthesis which are much more efficient than homopolymeric tailing, but the sense of the method remains the same).
rapid amplification of cdna ends
restriction enzyme mediated integration









































restriction enzyme mediated integration (abbreviated as remi) is a technique for integrating dna (linearised plasmid) into the genome sites that have been generated by the same restriction enzyme used for the dna linearisation. the plasmid integration occurs at the corresponding sites in the genome, often by regenerating the recognition sites by same the restriction enzyme used for plasmid linearisation.









































section::::mechanism.
restriction enzyme mediated integration