 In wild type "Gryllus bimaculatus," "tim" mRNA shows rhythmic expression in both LD and DD (dark-dark cycles) similar to that of "per", peaking during the subjective night.When injected with "tim" double-stranded RNA ("dstim"), "tim" mRNA levels were significantly reduced and its circadian expression rhythm was eliminated.After the "dstim" treatment, however, adult crickets showed a clear locomotor rhythm in constant darkness, with a free-running period significantly shorter than that of control crickets injected with Discosoma sp.