 The lowest quantity of DNA that could be amplified from individual adults of "Opisthorchis viverrini" was estimated to 0.6 pg.Effective prevention could be readily achieved by persuading people to consume cooked fish (via education programs), but the ancient cultural custom to consume raw, undercooked or freshly pickled fish persists in endemic areas.One community health program, known as the "Lawa" model, has achieved success in the Lawa Lakes region south of Khon Kaen.