 the bait protein and possibly the prey protein.Binding makes the reflection angle change;

formula_7

As SPR biosensors facilitate measurements at different temperatures, thermodynamic analysis can be performed
to obtain a better understanding of the studied interaction.By performing measurements at different temperatures,
typically between 4 and 40 °C, it is possible to relate association and dissociation rate constants with activation
energy and thereby obtain thermodynamic parameters including binding enthalpy, binding entropy,
Gibbs free energy and heat capacity.