 This causes the ON bipolar cell to lose its inhibition and become active (depolarized), while the OFF bipolar cell loses its excitation (becomes hyperpolarized) and becomes silent.Rod bipolar cells do not synapse directly on to ganglion cells.Instead, rod bipolar cells synapse on to a Retina amacrine cell, which in turn excite cone ON bipolar cells (via gap junctions) and inhibit cone OFF bipolar cells (via glycine-mediated inhibitory synapses) thereby overtaking the cone pathway in order to send signals to ganglion cells at scotopic (low) ambient light conditions.