 After the insertion of Okazaki fragments, the RNA primers are removed (the mechanism of removal differs between prokaryotes and eukaryotes) and replaced with new deoxyribonucleotides that fill the gaps where the RNA was present.DNA ligase then joins the fragmented strands together, completing the synthesis of the lagging strand.In prokaryotes, DNA polymerase I synthesizes the Okazaki fragment until it reaches the previous RNA primer.