 Demethylation of CpGs in a gene promoter by TET enzyme activity increases transcription of the gene.The DNA binding sites of 519 transcription factors were evaluated.Of these, 169 transcription factors (33%) did not have CpG dinucleotides in their binding sites, and 33 transcription factors (6%) could bind to a CpG-containing motif but did not display a preference for a binding site with either a methylated or unmethylated CpG.