 The electrokinetic phenomenon of electrophoresis was observed for the first time in 1807 by Russian professors Peter Ivanovich Strakhov and Ferdinand Frederic Reuss at Moscow University, who noticed that the application of a constant electric field caused clay particles dispersed in water to migrate.It is ultimately caused by the presence of a charged interface between the particle surface and the surrounding fluid.It is the basis for analytical techniques used in chemistry for separating molecules by size, charge, or binding affinity.