 The maximum resolution is improved by spreading the affected resonances over two dimensions, therefore more peaks are resolved, larger molecules can be observed and more NOE's can be observed in a single measurement.More importantly, when the molecular motion is in the intermediate or slow motional regimes when the NOE is either zero or negative, the steady-state NOE experiment fails to give results that can be related to internuclear distances.Nuclear Overhauser Effect Spectroscopy (NOESY) is a 2D NMR spectroscopic method used to identify nuclear spins undergoing cross-relaxation and to measure their cross-relaxation rates.