 These mutations most often occur at position R882 in the protein and this mutation may cause loss of function.DNMT3A mutations are associated with poor overall survival, suggesting that they have an important common effect on the potential of AML cells to cause lethal disease.It has also been found that "DNMT3A"-mutated cell lines exhibit transcriptome instability, in that they have much more erroneous RNA splicing as compared to their isogenic wildtype counterparts.