 RhoA's role in signal transduction mediation is also attributed to the establishment of tissue polarity in epidermal structures due to its actin polymerization to coordinate vesicular motion; movement within actin filaments forms webs that move in conjunction with vesicular linear motion.As a result, mutations present in the polarity genes indicate that RhoA is critical for tissue polarity and directed intracellular movement.RhoA is required for processes involving cell development, some of which include outgrowth, dorsal closure, bone formation, and myogenesis.