 RhoA contains also four insertion or deletion sites with an extra helical subdomain; these sites are characteristic of many GTPases in the Rho family.Most importantly, RhoA contains two switch regions, Switch I and Switch II, whose conformational states are modified following the activation or inactivation of the protein.Both of these switches have characteristic folding, correspond to specific regions on the RhoA coil and are uniformly stabilized via hydrogen bonds.