 Like all ClC channels, ClC-5 needs to dimerize to create the pore through which the ions pass.ClC-5 can form both homo- and hetero-dimers due to its marked sequence homology with ClC-3 and ClC-4.The canonical 746-amino acid ClC-5 protein has 18 membrane spanning α-helices (named A to R), an intracellular N- terminal domain and a cytoplasmic C-terminus containing two cystathionine beta-synthase (CBS) domains which are known to be involved in the regulation of ClC-5 activity.