 In addition, GlyDH selectively oxidizes the C2 hydroxyl group to form a ketone rather than a terminal hydroxyl group to form an aldehyde.While the precise mechanism of this specific enzyme has not yet been characterized, kinetic studies support that GlyDH catalysis of the chemical reaction

is comparable to those of other alcohol dehydrogenases.Therefore, the following mechanism offers a reasonable representation of glycerol oxidation by NAD+.