 Experiments with Y27632 show it is a promising candidate as a therapeutic antihypertensive agent.Fasudil has been used to characterize the role of ROCK1 in vascular function in clinical studies and has been approved for use in Japan for treatment of cerebral vasospasm following subarachnoid hemorrhage.The ROCK1 signaling plays an important role in many diseases including diabetes, neurodegenerative diseases such as Parkinson's disease and amyotrophic lateral sclerosis (ALS), and pulmonary hypertension.